Comparison Log 2024-05-26 06:44:23.183406 mwtab Python Library Version: 1.2.5 Source: https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005196/mwtab/... Study ID: ST003167 Analysis ID: AN005196 Status: Inconsistent Sections "PROJECT" contain missmatched items: {('LABORATORY', "Laboratory of Angelo D''Alessandro in collaboration with Mercedes Rincon"), ('LABORATORY', "Laboratory of Angelo D'Alessandro in collaboration with Mercedes Rincon")} Sections "STUDY" contain missmatched items: {('LABORATORY', "Laboratory of Angelo D''Alessandro in collaboration with Mercedes Rincon"), ('LABORATORY', "Laboratory of Angelo D'Alessandro in collaboration with Mercedes Rincon")} Sections "TREATMENT" contain missmatched items: {('TREATMENT_SUMMARY', "The human CD19-BBz shRNA CAR lentiviral constructs were based on a previously described CD19-BBz CAR containing the human CD19-binding scFV FMC63, CD8 hinge domain, 4-1BB costimulatory domain and CD3 chain105. Using CD19-BBz CAR plasmid as a cloning vector, we generated multiple vectors where we incorporated the RNA polymerase III U6 promotor (on the 3' of the CD3 chain domain) followed by an shRNA: 1) a CD19-BBz/shMCJ-1 CAR construct containing the shMCJ-1 5’-GAAGATTTCAACTCCTAGC-3’ sequence106, 2) a CD19-BBz/shMCJ-2 CAR construct containing the shMCJ-2; 5’-AACCTCTAGAACAAGTTATC-3’, and 3) a CD19-BBz/c-shRNA CAR vectors expressing the shRNA encoding scramble sequences. Lentiviral supernatant was produced in the LentiX-293T packaging cell line (Clonetech) as previously described. Lentiviral supernatants were collected after 48 hours post-transfection."), ('TREATMENT_SUMMARY', "The human CD19-BBz shRNA CAR lentiviral constructs were based on a previously described CD19-BBz CAR containing the human CD19-binding scFV FMC63, CD8 hinge domain, 4-1BB costimulatory domain and CD3 chain105. Using CD19-BBz CAR plasmid as a cloning vector, we generated multiple vectors where we incorporated the RNA polymerase III U6 promotor (on the 3'' of the CD3 chain domain) followed by an shRNA: 1) a CD19-BBz/shMCJ-1 CAR construct containing the shMCJ-1 5’-GAAGATTTCAACTCCTAGC-3’ sequence106, 2) a CD19-BBz/shMCJ-2 CAR construct containing the shMCJ-2; 5’-AACCTCTAGAACAAGTTATC-3’, and 3) a CD19-BBz/c-shRNA CAR vectors expressing the shRNA encoding scramble sequences. Lentiviral supernatant was produced in the LentiX-293T packaging cell line (Clonetech) as previously described. Lentiviral supernatants were collected after 48 hours post-transfection.")}