Comparison Log
2025-03-23 07:36:16.439179
mwtab Python Library Version: 1.2.5
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006158/mwtab/...
Study ID:    ST003751
Analysis ID: AN006158
Status:      Inconsistent

Sections "CHROMATOGRAPHY" contain missmatched items: {('CHROMATOGRAPHY_SUMMARY', "The Agilent 1290 Infinity II Multisampler system was employed with a multiwash option, and 1 µL of extracted samples was injected. The multisampler temperature was maintained at 15 °C to ensure the stability of compounds and prevent lipid precipitation. For chromatographic separation, an Agilent InfinityLab Poroshell 120 ECC18 (3.0 × 100 mm, 2.7 µm) (Agilent Technologies) column and a compatible guard column (Agilent InfinityLab Poroshell 120 ECC18, 3.0 × 5 mm, 2.7 µm) were employed and held at 50 °C. The chromatography gradient was initiated at 70% of B at 0–1 min, increased to 86% at 3.5–10 min, and reached 100% B at 11–17 min. The initial conditions were restored by minute 17, followed by a 2-minute re-equilibration, resulting in a total running time of 19 min. The mobile phases for positive and negative ionization modes comprised (A) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 9:1 water/methanol ratio and (B) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 2:3:5 acetonitrile/methanol/isopropanol ratio. The flow rate was maintained at 0.6 mL/min. The multisampler''s multiwash strategy involved a methanol:isopropanol (50:50, v/v) mixture with a 15-second wash time and an aqueous:organic phases (30:70, v/v) mixture to achieve the initial conditions."), ('CHROMATOGRAPHY_SUMMARY', "The Agilent 1290 Infinity II Multisampler system was employed with a multiwash option, and 1 µL of extracted samples was injected. The multisampler temperature was maintained at 15 °C to ensure the stability of compounds and prevent lipid precipitation. For chromatographic separation, an Agilent InfinityLab Poroshell 120 ECC18 (3.0 × 100 mm, 2.7 µm) (Agilent Technologies) column and a compatible guard column (Agilent InfinityLab Poroshell 120 ECC18, 3.0 × 5 mm, 2.7 µm) were employed and held at 50 °C. The chromatography gradient was initiated at 70% of B at 0–1 min, increased to 86% at 3.5–10 min, and reached 100% B at 11–17 min. The initial conditions were restored by minute 17, followed by a 2-minute re-equilibration, resulting in a total running time of 19 min. The mobile phases for positive and negative ionization modes comprised (A) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 9:1 water/methanol ratio and (B) 10 mM ammonium acetate, 0.2 mM ammonium fluoride in a 2:3:5 acetonitrile/methanol/isopropanol ratio. The flow rate was maintained at 0.6 mL/min. The multisampler's multiwash strategy involved a methanol:isopropanol (50:50, v/v) mixture with a 15-second wash time and an aqueous:organic phases (30:70, v/v) mixture to achieve the initial conditions.")}
Sections "SAMPLEPREP" contain missmatched items: {('SAMPLEPREP_SUMMARY', 'PLASMA SAMPLES: Plasma samples underwent deproteinization and lipid extraction using an all-in-one single extraction method employing a solvent mixture composed of MeOH/MTBE/CHCl3 (4:3:3, v/v/v) for the isolation of non-polar lipids(1). The lipid extraction method started with thawing the samples on ice followed by a 2 min vortex homogenization. Subsequently, 40 µL of the plasma sample was mixed with 800 µL of the solvent mixture – also containing the internal standards (IS): 1 ppm C17-sphinganine and 2 ppm d31-palmitic acid –. The resulting mixture was vortex-mixed for 20 min followed by sample centrifugation at 16,100 x g for 10 min at 15 °C. Then, 300 µL of the supernatant were transferred to LC Chromacol (Thermo Fisher Scientific, Madrid, Sain) vials with insert and centrifuged at 16,100 x g for 10 min at 15 °C prior to the analysis. Blank solutions were prepared containing 40 µL of H2O and 800 µL of the solvent mixture and same procedure was followed. For quality control (QC) samples, three independent pools were prepared by pipetting 10 µL of each sample – for QCTOTAL –, 10 µL of Alport samples (cases 1 and 2) – for QCCASES –, and 10 µL of control samples (DM patients and healthy individuals) – for QCCONTROL–. Then 40 µL of each of the pools were transferred to Eppendorf™ tubes and 800 µL of the solvent mixture were added. The same procedure as in sample preparation was followed. Both blank and QC samples were processed in parallel with the rest of plasma samples. URINE SAMPLES: Urine samples were prepared at the "Centro de Metabolómica y Bioanálisis, CEMBIO" (Madrid, Spain), following a monophasic extraction method (MeOH:EtOH (1:1, v/v)), tested and developed in our laboratory to obtain the urine lipidomics fingerprint. Briefly, the samples were thawed on ice and then vortex-mixed for 2 min. On ice, 100 µL of urine sample was mixed with 300 µL of a cold (-20 °C) mixture of MeOH:EtOH (1:1, v/v), and a mixture of nonendogenous internal standards (C17-Sphinganine at 1 ppm and d31-palmitic acid at 2 ppm). Samples were vortex-mixed for 30 minutes. After centrifugation for 10 min at 16.1 rpm at 15 °C, 100 µL of the supernatant was collected and transferred into an HPLC-MS vial with a glass insert. Finally, all the vials were centrifuged at 2000g at 15 °C for 10 min before injecting into the system.'), ('SAMPLEPREP_SUMMARY', 'PLASMA SAMPLES: Plasma samples underwent deproteinization and lipid extraction using an all-in-one single extraction method employing a solvent mixture composed of MeOH/MTBE/CHCl3 (4:3:3, v/v/v) for the isolation of non-polar lipids(1). The lipid extraction method started with thawing the samples on ice followed by a 2 min vortex homogenization. Subsequently, 40 µL of the plasma sample was mixed with 800 µL of the solvent mixture – also containing the internal standards (IS): 1 ppm C17-sphinganine and 2 ppm d31-palmitic acid –. The resulting mixture was vortex-mixed for 20 min followed by sample centrifugation at 16,100 x g for 10 min at 15 °C. Then, 300 µL of the supernatant were transferred to LC Chromacol (Thermo Fisher Scientific, Madrid, Sain) vials with insert and centrifuged at 16,100 x g for 10 min at 15 °C prior to the analysis. Blank solutions were prepared containing 40 µL of H2O and 800 µL of the solvent mixture and same procedure was followed. For quality control (QC) samples, three independent pools were prepared by pipetting 10 µL of each sample – for QCTOTAL –, 10 µL of Alport samples (cases 1 and 2) – for QCCASES –, and 10 µL of control samples (DM patients and healthy individuals) – for QCCONTROL–. Then 40 µL of each of the pools were transferred to Eppendorf™ tubes and 800 µL of the solvent mixture were added. The same procedure as in sample preparation was followed. Both blank and QC samples were processed in parallel with the rest of plasma samples. URINE SAMPLES: Urine samples were prepared at the Centro de Metabolómica y Bioanálisis, CEMBIO (Madrid, Spain), following a monophasic extraction method (MeOH:EtOH (1:1, v/v)), tested and developed in our laboratory to obtain the urine lipidomics fingerprint. Briefly, the samples were thawed on ice and then vortex-mixed for 2 min. On ice, 100 µL of urine sample was mixed with 300 µL of a cold (-20 °C) mixture of MeOH:EtOH (1:1, v/v), and a mixture of nonendogenous internal standards (C17-Sphinganine at 1 ppm and d31-palmitic acid at 2 ppm). Samples were vortex-mixed for 30 minutes. After centrifugation for 10 min at 16.1 rpm at 15 °C, 100 µL of the supernatant was collected and transferred into an HPLC-MS vial with a glass insert. Finally, all the vials were centrifuged at 2000g at 15 °C for 10 min before injecting into the system.')}
Sections "TREATMENT" contain missmatched items: {('TREATMENT_SUMMARY', 'PLASMA SAMPLES: Plasma samples underwent deproteinization and lipid extraction using an all-in-one single extraction method employing a solvent mixture composed of MeOH/MTBE/CHCl3 (4:3:3, v/v/v) for the isolation of non-polar lipids(1). The lipid extraction method started with thawing the samples on ice followed by a 2 min vortex homogenization. Subsequently, 40 µL of the plasma sample was mixed with 800 µL of the solvent mixture – also containing the internal standards (IS): 1 ppm C17-sphinganine and 2 ppm d31-palmitic acid –. The resulting mixture was vortex-mixed for 20 min followed by sample centrifugation at 16,100 x g for 10 min at 15 °C. Then, 300 µL of the supernatant were transferred to LC Chromacol (Thermo Fisher Scientific, Madrid, Sain) vials with insert and centrifuged at 16,100 x g for 10 min at 15 °C prior to the analysis. Blank solutions were prepared containing 40 µL of H2O and 800 µL of the solvent mixture and same procedure was followed. For quality control (QC) samples, three independent pools were prepared by pipetting 10 µL of each sample – for QCTOTAL –, 10 µL of Alport samples (cases 1 and 2) – for QCCASES –, and 10 µL of control samples (DM patients and healthy individuals) – for QCCONTROL–. Then 40 µL of each of the pools were transferred to Eppendorf™ tubes and 800 µL of the solvent mixture were added. The same procedure as in sample preparation was followed. Both blank and QC samples were processed in parallel with the rest of plasma samples. URINE SAMPLES: Urine samples were prepared at the Centro de Metabolómica y Bioanálisis, CEMBIO (Madrid, Spain), following a monophasic extraction method (MeOH:EtOH (1:1, v/v)), tested and developed in our laboratory to obtain the urine lipidomics fingerprint. Briefly, the samples were thawed on ice and then vortex-mixed for 2 min. On ice, 100 µL of urine sample was mixed with 300 µL of a cold (-20 °C) mixture of MeOH:EtOH (1:1, v/v), and a mixture of nonendogenous internal standards (C17-Sphinganine at 1 ppm and d31-palmitic acid at 2 ppm). Samples were vortex-mixed for 30 minutes. After centrifugation for 10 min at 16.1 rpm at 15 °C, 100 µL of the supernatant was collected and transferred into an HPLC-MS vial with a glass insert. Finally, all the vials were centrifuged at 2000g at 15 °C for 10 min before injecting into the system.'), ('TREATMENT_SUMMARY', 'PLASMA SAMPLES: Plasma samples underwent deproteinization and lipid extraction using an all-in-one single extraction method employing a solvent mixture composed of MeOH/MTBE/CHCl3 (4:3:3, v/v/v) for the isolation of non-polar lipids(1). The lipid extraction method started with thawing the samples on ice followed by a 2 min vortex homogenization. Subsequently, 40 µL of the plasma sample was mixed with 800 µL of the solvent mixture – also containing the internal standards (IS): 1 ppm C17-sphinganine and 2 ppm d31-palmitic acid –. The resulting mixture was vortex-mixed for 20 min followed by sample centrifugation at 16,100 x g for 10 min at 15 °C. Then, 300 µL of the supernatant were transferred to LC Chromacol (Thermo Fisher Scientific, Madrid, Sain) vials with insert and centrifuged at 16,100 x g for 10 min at 15 °C prior to the analysis. Blank solutions were prepared containing 40 µL of H2O and 800 µL of the solvent mixture and same procedure was followed. For quality control (QC) samples, three independent pools were prepared by pipetting 10 µL of each sample – for QCTOTAL –, 10 µL of Alport samples (cases 1 and 2) – for QCCASES –, and 10 µL of control samples (DM patients and healthy individuals) – for QCCONTROL–. Then 40 µL of each of the pools were transferred to Eppendorf™ tubes and 800 µL of the solvent mixture were added. The same procedure as in sample preparation was followed. Both blank and QC samples were processed in parallel with the rest of plasma samples. URINE SAMPLES: Urine samples were prepared at the "Centro de Metabolómica y Bioanálisis, CEMBIO" (Madrid, Spain), following a monophasic extraction method (MeOH:EtOH (1:1, v/v)), tested and developed in our laboratory to obtain the urine lipidomics fingerprint. Briefly, the samples were thawed on ice and then vortex-mixed for 2 min. On ice, 100 µL of urine sample was mixed with 300 µL of a cold (-20 °C) mixture of MeOH:EtOH (1:1, v/v), and a mixture of nonendogenous internal standards (C17-Sphinganine at 1 ppm and d31-palmitic acid at 2 ppm). Samples were vortex-mixed for 30 minutes. After centrifugation for 10 min at 16.1 rpm at 15 °C, 100 µL of the supernatant was collected and transferred into an HPLC-MS vial with a glass insert. Finally, all the vials were centrifuged at 2000g at 15 °C for 10 min before injecting into the system.')}