Validation Log 2025-12-14 21:13:00.141330 mwtab Python Library Version: 2.0.0 Source: https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN000317/mwtab/txt Study ID: ST000215 Analysis ID: AN000317 File format: txt Status: Contains Validation Issues Number of Issues: 21 Number of Warnings: 9 Number of Value Errors: 12 Number of Consistency Errors: 2 Number of Format Errors: 5 Issue Log: Error: An empty value or a null value was detected for the subsection, "PHONE", in the "STUDY" section. A legitimate value should be provided for this required subsection. Error: An empty value or a null value was detected for the subsection, "COLLECTION_SUMMARY", in the "COLLECTION" section. A legitimate value should be provided for this required subsection. Error: An empty value or a null value was detected for the subsection, "TREATMENT_SUMMARY", in the "TREATMENT" section. A legitimate value should be provided for this required subsection. Error: An empty value or a null value was detected for the subsection, "SAMPLEPREP_SUMMARY", in the "SAMPLEPREP" section. A legitimate value should be provided for this required subsection. Error: An empty value or a null value was detected for the subsection, "MS_COMMENTS", in the "MS" section. Either a legitimate value should be provided for this subsection, or it should be removed altogether. Error: The required property, "FLOW_GRADIENT", in the "CHROMATOGRAPHY" section is missing. Error: The required property, "FLOW_RATE", in the "CHROMATOGRAPHY" section is missing. Error: The required property, "COLUMN_TEMPERATURE", in the "CHROMATOGRAPHY" section is missing. Error: The required property, "SOLVENT_A", in the "CHROMATOGRAPHY" section is missing. Error: The required property, "SOLVENT_B", in the "CHROMATOGRAPHY" section is missing. Error: The following metabolites in the, MS_METABOLITE_DATA table were not found in the METABOLITES table: "3-Glycerophosphate_1" "3-Glycerophosphate_2" "3-Glycerophosphate_3" "3-phosphoglyceraldehyde_1" "3-phosphoglyceraldehyde_2" "3-phosphoglyceraldehyde_3" "Erythrose 4-phosphate_1" "Erythrose 4-phosphate_2" "Erythrose 4-phosphate_3" "Erythrose 4-phosphate_4" "Fructose 1_6-bisphosphate" "Fructose 1_6-bisphosphate_1" "Fructose 1_6-bisphosphate_2" "Fructose 1_6-bisphosphate_3" "Fructose 1_6-bisphosphate_4" "Fructose 1_6-bisphosphate_5" "Fructose 1_6-bisphosphate_6" "Glucose 6-phosphate or fructose-6-phosphate_1" "Glucose 6-phosphate or fructose-6-phosphate_2" "Glucose 6-phosphate or fructose-6-phosphate_3" "Glucose 6-phosphate or fructose-6-phosphate_4" "Glucose 6-phosphate or fructose-6-phosphate_5" "Glucose 6-phosphate or fructose-6-phosphate_6" "Glutamate_1" "Glutamate_2" "Glutamate_3" "Glutamate_4" "Glutamate_5" "Lactate_1" "Lactate_2" "Lactate_3" "Malate_1" "Malate_2" "Malate_3" "Malate_4" "Malonyl-CoA_1" "Malonyl-CoA_2" "Malonyl-CoA_3" "Nicotinamide Adenine Dinucleotide oxidized_1" "Nicotinamide Adenine Dinucleotide oxidized_2" "Nicotinamide Adenine Dinucleotide oxidized_3" "Nicotinamide Adenine Dinucleotide oxidized_4" "Nicotinamide Adenine Dinucleotide oxidized_5" "Nicotinamide Adenine Dinucleotide reduced_1" "Nicotinamide Adenine Dinucleotide reduced_2" "Nicotinamide Adenine Dinucleotide reduced_3" "Nicotinamide Adenine Dinucleotide reduced_4" "Nicotinamide Adenine Dinucleotide reduced_5" "Phosphoenolpyruvate_1" "Phosphoenolpyruvate_2" "Phosphoenolpyruvate_3" "Ribose-5-phosphate or xylulose 5-phosphate_1" "Ribose-5-phosphate or xylulose 5-phosphate_2" "Ribose-5-phosphate or xylulose 5-phosphate_3" "Ribose-5-phosphate or xylulose 5-phosphate_4" "Ribose-5-phosphate or xylulose 5-phosphate_5" "Sedoheptulose 7-phosphate_1" "Sedoheptulose 7-phosphate_2" "Sedoheptulose 7-phosphate_3" "Sedoheptulose 7-phosphate_4" "Sedoheptulose 7-phosphate_5" "Sedoheptulose 7-phosphate_6" "Sedoheptulose 7-phosphate_7" "Sucrose_1" "Sucrose_2" "Sucrose_3" "Sucrose_4" Error: The following metabolites in the METABOLITES table were not found in the MS_METABOLITE_DATA table: "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" Warning: The following metabolites in the METABOLITES table appear more than once in the table: "3-Glycerophosphate" "3-Glycerophosphate" "3-Glycerophosphate" "3-phosphoglyceraldehyde" "3-phosphoglyceraldehyde" "3-phosphoglyceraldehyde" "Erythrose 4-phosphate" "Erythrose 4-phosphate" "Erythrose 4-phosphate" "Erythrose 4-phosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Fructose 1,6-bisphosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glucose 6-phosphate or fructose-6-phosphate" "Glutamate" "Glutamate" "Glutamate" "Glutamate" "Glutamate" "Lactate" "Lactate" "Lactate" "Malate" "Malate" "Malate" "Malate" "Malonyl-CoA" "Malonyl-CoA" "Malonyl-CoA" "Nicotinamide Adenine Dinucleotide oxidized" "Nicotinamide Adenine Dinucleotide oxidized" "Nicotinamide Adenine Dinucleotide oxidized" "Nicotinamide Adenine Dinucleotide oxidized" "Nicotinamide Adenine Dinucleotide oxidized" "Nicotinamide Adenine Dinucleotide reduced" "Nicotinamide Adenine Dinucleotide reduced" "Nicotinamide Adenine Dinucleotide reduced" "Nicotinamide Adenine Dinucleotide reduced" "Nicotinamide Adenine Dinucleotide reduced" "Phosphoenolpyruvate" "Phosphoenolpyruvate" "Phosphoenolpyruvate" "Ribose-5-phosphate or xylulose 5-phosphate" "Ribose-5-phosphate or xylulose 5-phosphate" "Ribose-5-phosphate or xylulose 5-phosphate" "Ribose-5-phosphate or xylulose 5-phosphate" "Ribose-5-phosphate or xylulose 5-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sedoheptulose 7-phosphate" "Sucrose" "Sucrose" "Sucrose" "Sucrose" Warning: The "ri" column at position 7 in the METABOLITES table, matches a standard column name, "retention_index". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The "ri_type" column at position 8 in the METABOLITES table, matches a standard column name, "retention_index_type". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The column pair, "other_id" and "other_id_type", in the METABOLITES table should have data in the same rows, but at least one row has data in one column and nothing in the other. Warning: The standard column, "other_id", was found in the METABOLITES table as "other_id". If this column contains database IDs for standard databases such as KEGG, PubChem, HMDB, etc., it is recommended to make individual columns for these and not lump them together into a less descriptive "other_id" column. Warning: The "inchi_key" column at position 3 in the METABOLITES table has all null values. Warning: The "ri" column at position 7 in the METABOLITES table has all null values. Warning: The "ri_type" column at position 8 in the METABOLITES table has all null values. Warning: The "moverz_quant" column at position 9 in the METABOLITES table has all null values.