Validation Log 2025-12-14 21:17:37.617364 mwtab Python Library Version: 2.0.0 Source: https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN000374/mwtab/txt Study ID: ST000241 Analysis ID: AN000374 File format: txt Status: Contains Validation Issues Number of Issues: 16 Number of Warnings: 6 Number of Value Errors: 11 Number of Consistency Errors: 2 Number of Format Errors: 2 Issue Log: Error: An empty value or a null value was detected for the subsection, "SUBJECT_COMMENTS", in the "SUBJECT" section. Either a legitimate value should be provided for this subsection, or it should be removed altogether. Error: An empty value or a null value was detected for the subsection, "CELL_PASSAGE_NUMBER", in the "SUBJECT" section. Either a legitimate value should be provided for this subsection, or it should be removed altogether. Error: An empty value or a null value was detected for the subsection, "CELL_COUNTS", in the "SUBJECT" section. Either a legitimate value should be provided for this subsection, or it should be removed altogether. Error: An empty value or a null value was detected for the subsection, "COLLECTION_SUMMARY", in the "COLLECTION" section. A legitimate value should be provided for this required subsection. Error: The value, "24 hr", for the subsection, "TREATMENT_DOSEDURATION", in the "TREATMENT" section should be a number or range (ex. "5-6") followed by a space with a unit (ex. "5 V") from the following list: ['h', 'weeks', 'days']. Ignore this when more complicated descriptions are required. Error: An empty value or a null value was detected for the subsection, "SAMPLEPREP_SUMMARY", in the "SAMPLEPREP" section. A legitimate value should be provided for this required subsection. Error: The required property, "FLOW_GRADIENT", in the "CHROMATOGRAPHY" section is missing. Error: The required property, "COLUMN_TEMPERATURE", in the "CHROMATOGRAPHY" section is missing. Error: The following metabolites in the, MS_METABOLITE_DATA table were not found in the METABOLITES table: "3a_16b-Dihydroxyandrostenone" "DG(14:0/22:4(7Z_10Z_13Z_16Z)/0:0)" "DG(16:0/22:5(4Z_7Z_10Z_13Z_16Z)/0:0)" "DG(18:0/22:6(4Z_7Z_10Z_13Z_16Z_19Z)/0:0)" "DG(22:2(13Z_16Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z)/0:0)" "PA(20:4(5Z_8Z_11Z_14Z)e/2:0)" "PC(18:2(9Z_12Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PC(18:2(9Z_12Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))_1" "PC(18:2(9Z_12Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))_2" "PC(18:2(9Z_12Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))_3" "PC(20:4(5Z_8Z_11Z_14Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PC(20:4(5Z_8Z_11Z_14Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))_1" "PC(20:4(5Z_8Z_11Z_14Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))_2" "PC(o-22:0/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(14:1(9Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(18:4(6Z_9Z_12Z_15Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(20:3(5Z_8Z_11Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(20:4(5Z_8Z_11Z_14Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(20:5(5Z_8Z_11Z_14Z_17Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(22:4(7Z_10Z_13Z_16Z)/22:5(4Z_7Z_10Z_13Z_16Z))" "PE(22:5(4Z_7Z_10Z_13Z_16Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(22:6(4Z_7Z_10Z_13Z_16Z_19Z)/22:6(4Z_7Z_10Z_13Z_16Z_19Z))" "PE(O-18:1(1Z)/20:4(5Z_8Z_11Z_14Z))" "TG(16:0/16:1(9Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(16:0/18:1(9Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(16:0/18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(16:0/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]" "TG(16:1(9Z)/16:1(9Z)/20:4(5Z_8Z_11Z_14Z))[iso3]" "TG(16:1(9Z)/18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(16:1(9Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]" "TG(16:1(9Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]_1" "TG(18:0/18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(18:1(9Z)/18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z))[iso6]" "TG(18:1(9Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]" "TG(18:1(9Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]_1" "TG(18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]" "TG(18:2(9Z_12Z)/20:4(5Z_8Z_11Z_14Z)/20:4(5Z_8Z_11Z_14Z))[iso3]_1" "TG(18:3(9Z_12Z_15Z)/18:2(9Z_12Z)/18:3(9Z_12Z_15Z))[iso3]" "TG(18:3(9Z_12Z_15Z)/18:2(9Z_12Z)/18:3(9Z_12Z_15Z))[iso3]_1" "TG(20:4(5Z_8Z_11Z_14Z)/14:0/18:3(9Z_12Z_15Z))[iso6]" Error: The following metabolites in the METABOLITES table were not found in the MS_METABOLITE_DATA table: "3a,16b-Dihydroxyandrostenone" "DG(14:0/22:4(7Z,10Z,13Z,16Z)/0:0)" "DG(16:0/22:5(4Z,7Z,10Z,13Z,16Z)/0:0)" "DG(18:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)" "DG(22:2(13Z,16Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)" "PA(20:4(5Z,8Z,11Z,14Z)e/2:0)" "PC(18:2(9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PC(18:2(9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))_2" "PC(18:2(9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))_3" "PC(18:2(9Z,12Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))_4" "PC(20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PC(20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))_2" "PC(20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))_3" "PC(o-22:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(14:1(9Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(18:4(6Z,9Z,12Z,15Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(20:3(5Z,8Z,11Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(20:4(5Z,8Z,11Z,14Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(20:5(5Z,8Z,11Z,14Z,17Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(22:4(7Z,10Z,13Z,16Z)/22:5(4Z,7Z,10Z,13Z,16Z))" "PE(22:5(4Z,7Z,10Z,13Z,16Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(22:6(4Z,7Z,10Z,13Z,16Z,19Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z))" "PE(O-18:1(1Z)/20:4(5Z,8Z,11Z,14Z))" "TG(16:0/16:1(9Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(16:0/18:1(9Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(16:0/18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(16:0/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]" "TG(16:1(9Z)/16:1(9Z)/20:4(5Z,8Z,11Z,14Z))[iso3]" "TG(16:1(9Z)/18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(16:1(9Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]" "TG(16:1(9Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]_2" "TG(18:0/18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(18:1(9Z)/18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z))[iso6]" "TG(18:1(9Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]" "TG(18:1(9Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]_2" "TG(18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]" "TG(18:2(9Z,12Z)/20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))[iso3]_2" "TG(18:3(9Z,12Z,15Z)/18:2(9Z,12Z)/18:3(9Z,12Z,15Z))[iso3]" "TG(18:3(9Z,12Z,15Z)/18:2(9Z,12Z)/18:3(9Z,12Z,15Z))[iso3]_2" "TG(20:4(5Z,8Z,11Z,14Z)/14:0/18:3(9Z,12Z,15Z))[iso6]" Warning: The "ri" column at position 3 in the METABOLITES table, matches a standard column name, "retention_index". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The "ri_type" column at position 4 in the METABOLITES table, matches a standard column name, "retention_index_type". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The standard column, "other_id", was found in the METABOLITES table as "other_id". If this column contains database IDs for standard databases such as KEGG, PubChem, HMDB, etc., it is recommended to make individual columns for these and not lump them together into a less descriptive "other_id" column. Warning: The "inchi_key" column at position 6 in the METABOLITES table has all null values. Warning: The "other_id" column at position 8 in the METABOLITES table has all null values. Warning: The "other_id_type" column at position 9 in the METABOLITES table has all null values.