Validation Log 2025-12-14 21:18:28.997013 mwtab Python Library Version: 2.0.0 Source: https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN000392/mwtab/json Study ID: ST000248 Analysis ID: AN000392 File format: json Status: Contains Validation Issues Number of Issues: 19 Number of Warnings: 6 Number of Value Errors: 10 Number of Consistency Errors: 2 Number of Format Errors: 5 Issue Log: Error: An empty value or a null value was detected in ["PROJECT"]["ADDRESS"]. A legitimate value should be provided for this required key. Error: An empty value or a null value was detected in ["STUDY"]["PHONE"]. A legitimate value should be provided for this required key. Error: An empty value or a null value was detected in ["COLLECTION"]["COLLECTION_SUMMARY"]. A legitimate value should be provided for this required key. Error: An empty value or a null value was detected in ["TREATMENT"]["TREATMENT_SUMMARY"]. A legitimate value should be provided for this required key. Error: An empty value or a null value was detected in ["SAMPLEPREP"]["SAMPLEPREP_SUMMARY"]. A legitimate value should be provided for this required key. Error: An empty value or a null value was detected in ["MS"]["MS_COMMENTS"]. Either a legitimate value should be provided for this key, or it should be removed altogether. Error: The required property, "FLOW_GRADIENT", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "FLOW_RATE", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "COLUMN_TEMPERATURE", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "SOLVENT_A", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "SOLVENT_B", in ["CHROMATOGRAPHY"] is missing. Error: The following metabolites in the, ["MS_METABOLITE_DATA"]["Data"] table were not found in the ["MS_METABOLITE_DATA"]["Metabolites"] table: "10_16-Dihydroxyhexadecanoic acid_R1" "10_16-Dihydroxyhexadecanoic acid_R2" "10_16-Dihydroxyhexadecanoic acid_R3" "1-(4-carboxybutanamido)-1^-(dimethylcarbamoyl)ferrocene" "1_6-Naphthalenedisulfonic acid" "(1R_2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R1" "(1R_2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R2" "(1R_2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R3" "2_2-Dimethyl-3-[4-(acetyloxy)phenyl]-4-ethyl-2H-1-benzopyran-7-ol acetate" "2_3-Dihydroxycyclopentaneundecanoic acid" "2_3-Dihydroxy-p-cumate" "2_3-Dimethylmaleate" "2_4-Dichlorobenzoate" "2_4-Dinitrophenol" "2_5-Dioxopentanoate_R1" "2_5-Dioxopentanoate_R2" "2-Chloro-1_4-naphthoquinone" "2-Hydroxy-2_4-pentadienoate_R1" "2-Hydroxy-2_4-pentadienoate_R2" "2^^-O-Galloylisovitexin" "3-[(3aS_4S_7aS)-7a-Methyl-1_5-dioxo-octahydro-1H-inden-4-yl]propanoate" "3_4-Dihydroxyphenylethyleneglycol" "3_5_6_7_2^_3^_4^-Heptahydroxyflavone" "3_5-Dinitroguaiacol" "3_6-dioxo-decanoic acid_R1" "3_6-dioxo-decanoic acid_R2" "3_6-dioxo-decanoic acid_R3" "3^-Bromo-6^-hydroxy-2^_4_4^-trimethoxychalcone_R1" "3^-Bromo-6^-hydroxy-2^_4_4^-trimethoxychalcone_R2" "3^-CMP" "3E_5E-tridecadienoic acid_R1" "3E_5E-tridecadienoic acid_R2" "3-Hydroxy-2-methylpyridine-4_5-dicarboxylate" "4_4^-Diaminostilbene dihydrochloride_R1" "4_4^-Diaminostilbene dihydrochloride_R2" "4_7-dioxo-octanoic acid" "4^-Methoxychalcone" "5-Amino-6-(5^-phosphoribosylamino)uracil" "5-hydroperoxy-7-[3_5-epidioxy-2-(2-octenyl)-cyclopentyl]-6-heptenoic acid" "5-Oxo-1_2-campholide_R1" "5-Oxo-1_2-campholide_R2" "5-Oxo-1_2-campholide_R3" "5^^-Phosphoribostamycin" "(6S_9R)-Vomifoliol_R1" "(6S_9R)-Vomifoliol_R2" "9_12_13-TriHOME_R1" "9_12_13-TriHOME_R2" "9-Hydroxybenzo[a]pyrene-4_5-oxide" "(9R_10R)-Dihydroxyoctadecanoic acid_R1" "(9R_10R)-Dihydroxyoctadecanoic acid_R2" "(9R_10R)-Dihydroxyoctadecanoic acid_R3" "(9Z_12Z)-(8R)-Hydroxyoctadeca-9_12-dienoic acid" "(9Z)-(7S_8S)-Dihydroxyoctadecenoic acid_R1" "(9Z)-(7S_8S)-Dihydroxyoctadecenoic acid_R2" "ADENOSINE 5^-MONOPHOSPHATE" "alpha_beta-Didehydrotryptophan" "Benzyl (2R_3S)-2-methyl-3-hydroxybutanoate" "Catechin 5_7_3^-trimethyl ether" "cis-1_2-Dihydro-3-ethylcatechol" "cis-2_3-Dihydroxy-2_3-dihydro-p-cumate_R1" "cis-2_3-Dihydroxy-2_3-dihydro-p-cumate_R2" "D-Arabinono-1_4-lactone" "D-Glucono-1_5-lactone" "Dihydro-4_4-dimethyl-2_3-furandione_R1" "Dihydro-4_4-dimethyl-2_3-furandione_R2" "Dihydro-4_4-dimethyl-2_3-furandione_R3" "D-myo-Inositol 1_2-cyclic phosphate" "Ethyl(E_Z)-decadienoate" "INOSINE 5^-PHOSPHATE" "L-2_3-Dihydrodipicolinate" "PC(15:0/18:2(9Z_12Z))" "PE(18:2(9Z_12Z)/0:0)" "PE(20:3(8Z_11Z_14Z)/0:0)" "PS(20:3(8Z_11Z_14Z)/0:0)" "PS(20:4(5Z_8Z_11Z_14Z)/0:0)" "Tryptophan-2_3_3-D3" "Xanthosine 5^-phosphate" Error: The following metabolites in the ["MS_METABOLITE_DATA"]["Metabolites"] table were not found in the ["MS_METABOLITE_DATA"]["Data"] table: "10,16-Dihydroxyhexadecanoic acid_R1" "10,16-Dihydroxyhexadecanoic acid_R2" "10,16-Dihydroxyhexadecanoic acid_R3" "1-(4-carboxybutanamido)-1'-(dimethylcarbamoyl)ferrocene" "1,6-Naphthalenedisulfonic acid" "(1R,2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R1" "(1R,2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R2" "(1R,2R)-3-oxo-2-pentyl-cyclopentanehexanoic acid_R3" "2,2-Dimethyl-3-[4-(acetyloxy)phenyl]-4-ethyl-2H-1-benzopyran-7-ol acetate" "2,3-Dihydroxycyclopentaneundecanoic acid" "2,3-Dihydroxy-p-cumate" "2,3-Dimethylmaleate" "2,4-Dichlorobenzoate" "2,4-Dinitrophenol" "2,5-Dioxopentanoate_R1" "2,5-Dioxopentanoate_R2" "2-Chloro-1,4-naphthoquinone" "2-Hydroxy-2,4-pentadienoate_R1" "2-Hydroxy-2,4-pentadienoate_R2" "2''-O-Galloylisovitexin" "3-[(3aS,4S,7aS)-7a-Methyl-1,5-dioxo-octahydro-1H-inden-4-yl]propanoate" "3,4-Dihydroxyphenylethyleneglycol" "3,5,6,7,2',3',4'-Heptahydroxyflavone" "3,5-Dinitroguaiacol" "3,6-dioxo-decanoic acid_R1" "3,6-dioxo-decanoic acid_R2" "3,6-dioxo-decanoic acid_R3" "3'-Bromo-6'-hydroxy-2',4,4'-trimethoxychalcone_R1" "3'-Bromo-6'-hydroxy-2',4,4'-trimethoxychalcone_R2" "3'-CMP" "3E,5E-tridecadienoic acid_R1" "3E,5E-tridecadienoic acid_R2" "3-Hydroxy-2-methylpyridine-4,5-dicarboxylate" "4,4'-Diaminostilbene dihydrochloride_R1" "4,4'-Diaminostilbene dihydrochloride_R2" "4,7-dioxo-octanoic acid" "4'-Methoxychalcone" "5-Amino-6-(5'-phosphoribosylamino)uracil" "5-hydroperoxy-7-[3,5-epidioxy-2-(2-octenyl)-cyclopentyl]-6-heptenoic acid" "5-Oxo-1,2-campholide_R1" "5-Oxo-1,2-campholide_R2" "5-Oxo-1,2-campholide_R3" "5''-Phosphoribostamycin" "(6S,9R)-Vomifoliol_R1" "(6S,9R)-Vomifoliol_R2" "9,12,13-TriHOME_R1" "9,12,13-TriHOME_R2" "9-Hydroxybenzo[a]pyrene-4,5-oxide" "(9R,10R)-Dihydroxyoctadecanoic acid_R1" "(9R,10R)-Dihydroxyoctadecanoic acid_R2" "(9R,10R)-Dihydroxyoctadecanoic acid_R3" "(9Z,12Z)-(8R)-Hydroxyoctadeca-9,12-dienoic acid" "(9Z)-(7S,8S)-Dihydroxyoctadecenoic acid_R1" "(9Z)-(7S,8S)-Dihydroxyoctadecenoic acid_R2" "ADENOSINE 5'-MONOPHOSPHATE" "alpha,beta-Didehydrotryptophan" "Benzyl (2R,3S)-2-methyl-3-hydroxybutanoate" "Catechin 5,7,3'-trimethyl ether" "cis-1,2-Dihydro-3-ethylcatechol" "cis-2,3-Dihydroxy-2,3-dihydro-p-cumate_R1" "cis-2,3-Dihydroxy-2,3-dihydro-p-cumate_R2" "D-Arabinono-1,4-lactone" "D-Glucono-1,5-lactone" "Dihydro-4,4-dimethyl-2,3-furandione_R1" "Dihydro-4,4-dimethyl-2,3-furandione_R2" "Dihydro-4,4-dimethyl-2,3-furandione_R3" "D-myo-Inositol 1,2-cyclic phosphate" "Ethyl(E,Z)-decadienoate" "INOSINE 5'-PHOSPHATE" "L-2,3-Dihydrodipicolinate" "PC(15:0/18:2(9Z,12Z))" "PE(18:2(9Z,12Z)/0:0)" "PE(20:3(8Z,11Z,14Z)/0:0)" "PS(20:3(8Z,11Z,14Z)/0:0)" "PS(20:4(5Z,8Z,11Z,14Z)/0:0)" "Tryptophan-2,3,3-D3" "Xanthosine 5'-phosphate" Warning: The "ri" column at position 3 in the ["MS_METABOLITE_DATA"]["Metabolites"] table, matches a standard column name, "retention_index". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The "ri_type" column at position 4 in the ["MS_METABOLITE_DATA"]["Metabolites"] table, matches a standard column name, "retention_index_type". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The column pair, "other_id" and "other_id_type", in the METABOLITES table should have data in the same rows, but at least one row has data in one column and nothing in the other. Warning: The standard column, "other_id", was found in the METABOLITES table as "other_id". If this column contains database IDs for standard databases such as KEGG, PubChem, HMDB, etc., it is recommended to make individual columns for these and not lump them together into a less descriptive "other_id" column. Warning: The "inchi_key" column at position 6 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "other_id" column at position 8 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values.