Validation Log 2025-12-15 00:18:31.232711 mwtab Python Library Version: 2.0.0 Source: https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN003414/mwtab/json Study ID: ST002090 Analysis ID: AN003414 File format: json Status: Contains Validation Issues Number of Issues: 20 Number of Warnings: 12 Number of Value Errors: 12 Number of Consistency Errors: 2 Number of Format Errors: 5 Issue Log: Error: The required property, "FLOW_GRADIENT", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "FLOW_RATE", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "COLUMN_TEMPERATURE", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "SOLVENT_A", in ["CHROMATOGRAPHY"] is missing. Error: The required property, "SOLVENT_B", in ["CHROMATOGRAPHY"] is missing. Error: The following metabolites in the, ["MS_METABOLITE_DATA"]["Data"] table were not found in the ["MS_METABOLITE_DATA"]["Metabolites"] table: "1_1-kestotetraose" "12_13-DiHOME" "1_2_3-benzenetriol sulfate (2)" "1_2-dilinolenoyl-digalactosylglycerol (18:3/18:3)" "1_2-dilinolenoyl-galactosylglycerol (18:3/18:3)*" "1_2-dilinoleoyl-digalactosylglycerol (18:2/18:2)*" "1_2-dilinoleoyl-galactosylglycerol (18:2/18:2)*" "1_2-dilinoleoyl-GPC (18:2/18:2)" "1_2-dilinoleoyl-GPE (18:2/18:2)*" "1_2-dipalmitoyl-GPC (16:0/16:0)" "1_5-anhydroglucitol (1_5-AG)" "2_3-dihydroxy-3-methylvalerate" "2_3-dihydroxyisovalerate" "2_4_6-trihydroxybenzoate" "2_4-dihydroxybutyrate" "2_6-dihydroxybenzoic acid" "2_8-quinolinediol sulfate" "2^-deoxyadenosine" "2^-deoxycytidine" "2^-deoxycytidine 5^-monophosphate" "2^-deoxyguanosine" "2^-deoxyinosine" "2^-deoxyuridine" "2^-O-methylcytidine" "2^-O-methyluridine" "2R_3R-dihydroxybutyrate" "3_4-dihydroxybutyrate" "3_5-dihydroxyphenylpropionate" "5_6-dihydrothymine" "5_6-dihydrouridine" "5alpha-pregnan-3beta_20alpha-diol disulfate" "5-methyl-2^-deoxycytidine" "9_10-DiHOME" "adenosine 5^-monophosphate (AMP)" "bilirubin degradation product_ C16H18N2O5 (4)**" "bilirubin degradation product_ C17H18N2O4 (1)**" "bilirubin degradation product_ C17H18N2O4 (2)**" "bilirubin degradation product_ C17H18N2O4 (3)**" "bilirubin degradation product_ C17H20N2O5 (1)**" "bilirubin degradation product_ C17H20N2O5 (2)**" "bilirubin (E_E)*" "bilirubin (Z_Z)" "ceramide (d18:1/14:0_ d16:1/16:0)*" "ceramide (d18:1/17:0_ d17:1/18:0)*" "ceramide (d18:1/20:0_ d16:1/22:0_ d20:1/18:0)*" "chrysoeriol (3^-O-methylluteolin)" "cyclo(his-pro) (D_L)*" "cytidine 5^-monophosphate (5^-CMP)" "diacylglycerol (16:1/18:2 [2]_ 16:0/18:3 [1])*" "glutamate_ gamma-methyl ester" "N1_N10-dicoumaroylspermidine" "N1_N12-diacetylspermine" "N2_N2-dimethylguanosine" "N2_N6-diacetyllysine" "N6_N6_N6-trimethyllysine" "N_N-dimethyl-5-aminovalerate" "N_N-dimethylalanine" "N_N-dimethyl-pro-pro" "N_N-dimethylvaline" "N_N_N-trimethyl-5-aminovalerate" "N_N_N-trimethyl-alanylproline betaine (TMAP)" "sphingomyelin (d17:1/16:0_ d18:1/15:0_ d16:1/17:0)*" "sphingomyelin (d18:1/14:0_ d16:1/16:0)*" "sphingomyelin (d18:1/22:1_ d18:2/22:0_ d16:1/24:1)*" "sphingomyelin (d18:1/24:1_ d18:2/24:0)*" "trigonelline (N^-methylnicotinate)" "uridine-2^_3^-cyclic monophosphate" "uridine 5^-monophosphate (UMP)" Error: The following metabolites in the ["MS_METABOLITE_DATA"]["Metabolites"] table were not found in the ["MS_METABOLITE_DATA"]["Data"] table: "1,1-kestotetraose" "12,13-DiHOME" "1,2,3-benzenetriol sulfate (2)" "1,2-dilinolenoyl-digalactosylglycerol (18:3/18:3)" "1,2-dilinolenoyl-galactosylglycerol (18:3/18:3)*" "1,2-dilinoleoyl-digalactosylglycerol (18:2/18:2)*" "1,2-dilinoleoyl-galactosylglycerol (18:2/18:2)*" "1,2-dilinoleoyl-GPC (18:2/18:2)" "1,2-dilinoleoyl-GPE (18:2/18:2)*" "1,2-dipalmitoyl-GPC (16:0/16:0)" "1,5-anhydroglucitol (1,5-AG)" "2,3-dihydroxy-3-methylvalerate" "2,3-dihydroxyisovalerate" "2,4,6-trihydroxybenzoate" "2,4-dihydroxybutyrate" "2,6-dihydroxybenzoic acid" "2,8-quinolinediol sulfate" "2'-deoxyadenosine" "2'-deoxycytidine" "2'-deoxycytidine 5'-monophosphate" "2'-deoxyguanosine" "2'-deoxyinosine" "2'-deoxyuridine" "2'-O-methylcytidine" "2'-O-methyluridine" "2R,3R-dihydroxybutyrate" "3,4-dihydroxybutyrate" "3,5-dihydroxyphenylpropionate" "5,6-dihydrothymine" "5,6-dihydrouridine" "5alpha-pregnan-3beta,20alpha-diol disulfate" "5-methyl-2'-deoxycytidine" "9,10-DiHOME" "adenosine 5'-monophosphate (AMP)" "bilirubin degradation product, C16H18N2O5 (4)**" "bilirubin degradation product, C17H18N2O4 (1)**" "bilirubin degradation product, C17H18N2O4 (2)**" "bilirubin degradation product, C17H18N2O4 (3)**" "bilirubin degradation product, C17H20N2O5 (1)**" "bilirubin degradation product, C17H20N2O5 (2)**" "bilirubin (E,E)*" "bilirubin (Z,Z)" "ceramide (d18:1/14:0, d16:1/16:0)*" "ceramide (d18:1/17:0, d17:1/18:0)*" "ceramide (d18:1/20:0, d16:1/22:0, d20:1/18:0)*" "chrysoeriol (3'-O-methylluteolin)" "cyclo(his-pro) (D,L)*" "cytidine 5'-monophosphate (5'-CMP)" "diacylglycerol (16:1/18:2 [2], 16:0/18:3 [1])*" "glutamate, gamma-methyl ester" "N1,N10-dicoumaroylspermidine" "N1,N12-diacetylspermine" "N2,N2-dimethylguanosine" "N2,N6-diacetyllysine" "N6,N6,N6-trimethyllysine" "N,N-dimethyl-5-aminovalerate" "N,N-dimethylalanine" "N,N-dimethyl-pro-pro" "N,N-dimethylvaline" "N,N,N-trimethyl-5-aminovalerate" "N,N,N-trimethyl-alanylproline betaine (TMAP)" "sphingomyelin (d17:1/16:0, d18:1/15:0, d16:1/17:0)*" "sphingomyelin (d18:1/14:0, d16:1/16:0)*" "sphingomyelin (d18:1/22:1, d18:2/22:0, d16:1/24:1)*" "sphingomyelin (d18:1/24:1, d18:2/24:0)*" "trigonelline (N'-methylnicotinate)" "uridine-2',3'-cyclic monophosphate" "uridine 5'-monophosphate (UMP)" Warning: The "ri" column at position 7 in the ["MS_METABOLITE_DATA"]["Metabolites"] table, matches a standard column name, "retention_index". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The "ri_type" column at position 8 in the ["MS_METABOLITE_DATA"]["Metabolites"] table, matches a standard column name, "retention_index_type". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name. Warning: The standard column, "other_id", was found in the METABOLITES table as "other_id". If this column contains database IDs for standard databases such as KEGG, PubChem, HMDB, etc., it is recommended to make individual columns for these and not lump them together into a less descriptive "other_id" column. Error: Column(s) with no name were found in the ["MS_METABOLITE_DATA"]["Metabolites"] table. Warning: The "pubchem_id" column at position 2 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "inchi_key" column at position 3 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "kegg_id" column at position 4 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "other_id" column at position 5 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "other_id_type" column at position 6 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "ri" column at position 7 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "ri_type" column at position 8 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "moverz_quant" column at position 9 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values. Warning: The "" column at position 10 in the ["MS_METABOLITE_DATA"]["Metabolites"] table has all null values.