Validation Log
2025-12-15 01:03:12.875658
mwtab Python Library Version: 2.0.0
Source:        https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN004259/mwtab/txt
Study ID:      ST002587
Analysis ID:   AN004259
File format:   txt
Status: Contains Validation Issues
Number of Issues: 18

Number of Warnings: 11

Number of Value Errors: 15

Number of Consistency Errors: 2

Number of Format Errors: 0

Issue Log:
Error: An empty value or a null value was detected for the subsection, "FLOW_GRADIENT", in the "CHROMATOGRAPHY" section. A legitimate value should be provided for this required subsection.
Error: The value, "0.69 ml/min", for the subsection, "FLOW_RATE", in the "CHROMATOGRAPHY" section should be a number or range (ex. "5-6") followed by a space with a unit (ex. "5 V") from the following list: ['mL/min', 'uL/min', 'μL/min']. Ignore this when more complicated descriptions are required.
Error: The value, "60-320", for the subsection, "COLUMN_TEMPERATURE", in the "CHROMATOGRAPHY" section should be a number or range (ex. "5-6") followed by a space with a unit (ex. "5 V") from the following list: ['°C', 'C']. Ignore this when more complicated descriptions are required.
Error: An empty value or a null value was detected for the subsection, "SOLVENT_A", in the "CHROMATOGRAPHY" section. A legitimate value should be provided for this required subsection.
Error: An empty value or a null value was detected for the subsection, "SOLVENT_B", in the "CHROMATOGRAPHY" section. A legitimate value should be provided for this required subsection.
Error: The following metabolites in the, MS_METABOLITE_DATA table were not found in the METABOLITES table:
	"2-hydroxyglutaric (433_245)"
	"2-phosphoGlycertate (585_383)"
	"3-hydroxybutyrate (275_233)"
	"3-Hydroxy isobutyric (275_260)"
	"3-phosphoglycerate (585_383)"
	"aketoglutarate (346_258)"
	"Alanine (260_232)"
	"Asparagine (417_302)"
	"Aspartic (418_390)"
	"Cis-aconitic (459_327)"
	"Citric (459_431)"
	"Citric m+1 (460_432)"
	"Citric m+2 (461_433)"
	"Citric m+3 (462_434)"
	"Citric m+4 (463_435)"
	"Citric m+5 (464_436)"
	"Citric m+6 (465_ 437)"
	"Creatinine (284_341)"
	"Cysteine (406_378)"
	"DHAP 1/2 (484_452_383)"
	"DHAP 2/2 (484_452_383)"
	"Ethanolamine (232_144)"
	"Fumaric (287_329)"
	"Glutamic (432_330)"
	"Glutamine (431_329)"
	"Glyceraldehyde-3P (484_457_425)"
	"Glycine (246_218)"
	"Glycolic Acid (247_189) ?"
	"Histidine (440_338)"
	"Homocysteine (420_392)"
	"Hydroxyproline (416_388)"
	"Hypotaruine (280_158)"
	"Isocitric (459_431)"
	"Isocitric m+1 (460_432)"
	"Isocitric m+2 (461_433)"
	"Isocitric m+3 (462_434)"
	"Isocitric m+4 (463_435)"
	"Isocitric m+5 (464_436)"
	"Isocitric m+6 (465_ 437)"
	"Isoleucine (302_274)"
	"Lactic (261_233)"
	"Leucine (302_274)"
	"Lysine (431_488)"
	"Malic (419_287)"
	"Methionine (320_292)"
	"Myristic M+0 (285_129_327)"
	"Niacinamide (179_ 136_ 221)"
	"Ornithine (474_417)"
	"Oxaloacetic (332_374)"
	"Phenylalanine (336_308)"
	"Phosphoenolpyruvic (453_495)"
	"Phosphoserine (584_482)"
	"Proline (286_258)"
	"Propylene glycol 2TBDMS 247_189 ?"
	"Pyroglutamic (300_272)"
	"Pyruvic (174_115)"
	"Serine (390_362)"
	"Stearic M+0 341_129_383"
	"Succinic (289_331)"
	"Threonine (404_376)"
	"Tyrosine (466_302)"
	"Urea (231_ 273)"
	"Uric acid (576_395_511)"
Error: The following metabolites in the METABOLITES table were not found in the MS_METABOLITE_DATA table:
	"2-hydroxyglutaric (433,245)"
	"2-phosphoGlycertate (585,383)"
	"3-hydroxybutyrate (275,233)"
	"3-Hydroxy isobutyric (275,260)"
	"3-phosphoglycerate (585,383)"
	"aketoglutarate (346,258)"
	"Alanine (260,232)"
	"Asparagine (417,302)"
	"Aspartic (418,390)"
	"Cis-aconitic (459,327)"
	"Citric (459,431)"
	"Citric m+1 (460,432)"
	"Citric m+2 (461,433)"
	"Citric m+3 (462,434)"
	"Citric m+4 (463,435)"
	"Citric m+5 (464,436)"
	"Citric m+6 (465, 437)"
	"Creatinine (284,341)"
	"Cysteine (406,378)"
	"DHAP 1/2 (484,452,383)"
	"DHAP 2/2 (484,452,383)"
	"Ethanolamine (232,144)"
	"Fumaric (287,329)"
	"Glutamic (432,330)"
	"Glutamine (431,329)"
	"Glyceraldehyde-3P (484,457,425)"
	"Glycine (246,218)"
	"Glycolic Acid (247,189) ?"
	"Histidine (440,338)"
	"Homocysteine (420,392)"
	"Hydroxyproline (416,388)"
	"Hypotaruine (280,158)"
	"Isocitric (459,431)"
	"Isocitric m+1 (460,432)"
	"Isocitric m+2 (461,433)"
	"Isocitric m+3 (462,434)"
	"Isocitric m+4 (463,435)"
	"Isocitric m+5 (464,436)"
	"Isocitric m+6 (465, 437)"
	"Isoleucine (302,274)"
	"Lactic (261,233)"
	"Leucine (302,274)"
	"Lysine (431,488)"
	"Malic (419,287)"
	"Methionine (320,292)"
	"Myristic M+0 (285,129,327)"
	"Niacinamide (179, 136, 221)"
	"Ornithine (474,417)"
	"Oxaloacetic (332,374)"
	"Phenylalanine (336,308)"
	"Phosphoenolpyruvic (453,495)"
	"Phosphoserine (584,482)"
	"Proline (286,258)"
	"Propylene glycol 2TBDMS 247,189 ?"
	"Pyroglutamic (300,272)"
	"Pyruvic (174,115)"
	"Serine (390,362)"
	"Stearic M+0 341,129,383"
	"Succinic (289,331)"
	"Threonine (404,376)"
	"Tyrosine (466,302)"
	"Urea (231, 273)"
	"Uric acid (576,395,511)"
Warning: The "ri" column at position 7 in the METABOLITES table, matches a standard column name, "retention_index". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name.
Warning: The "ri_type" column at position 8 in the METABOLITES table, matches a standard column name, "retention_index_type". If this match was not in error, the column should be renamed to the standard name or a name that doesn't resemble the standard name.
Warning: The standard column, "other_id", was found in the METABOLITES table as "other_id". If this column contains database IDs for standard databases such as KEGG, PubChem, HMDB, etc., it is recommended to make individual columns for these and not lump them together into a less descriptive "other_id" column.
Warning: The "pubchem_id" column at position 2 in the METABOLITES table has all null values.
Warning: The "inchi_key" column at position 3 in the METABOLITES table has all null values.
Warning: The "kegg_id" column at position 4 in the METABOLITES table has all null values.
Warning: The "other_id" column at position 5 in the METABOLITES table has all null values.
Warning: The "other_id_type" column at position 6 in the METABOLITES table has all null values.
Warning: The "ri" column at position 7 in the METABOLITES table has all null values.
Warning: The "ri_type" column at position 8 in the METABOLITES table has all null values.
Warning: The "moverz_quant" column at position 9 in the METABOLITES table has all null values.