Comparison Log
2025-12-15 01:54:54.818561
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN005069/mwtab/...
Study ID:    ST003098
Analysis ID: AN005069
Status:      Inconsistent

Sections "PROJECT" contain missmatched keys: ['DOI']
Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ['Cecum content was collected and stored at -80°C environment for tryptophan analysis.', 'cecum content sample was collected and stored at -80°C environment']}
Sections "SAMPLEPREP" contain missmatched items: {'SAMPLEPREP_SUMMARY': ['Sample was extracted in 100 μL of 80% methanol aqueous solution, high throughput tissue grinder grinding for 60 s, then add 900 μL of 10% methanol aqueous solution, high throughput tissue grinder grinding for 120 s. Centrifugation was carried out at 12,000 rpm at 4 ℃ for 10 min, and the supernatant was filtered through the membrane to add an equal amount of the internal standard solution, and then transferred into the vial.', 'sample was extracted in 100 μL of 80% methanol, and were centrifuged at 4 °C for 10 min at 12000 rpm after vortexing for 120s and the supernatant was resolved in water and filtered and then transferred into the vial']}
Sections "CHROMATOGRAPHY" contain missmatched items: {'INSTRUMENT_NAME': ['Thermo Vanquish', 'Waters Acquity'], 'FLOW_GRADIENT': ['0-2 min, 1% B; 2-3 min, 1-30% B; 3-3.5 min, 30% B; 4.5-8 min, 30-50% B; 8-10 min, 50-95% B; 10-11 min, 95% B; 11-17 min, 95-1% B.', '1% B； 2~3 min， 1~30% B； 3~3.5 min， 30% B； 4.5~8 min， 30~50% B； 8~10 min， 50~95% B； 10~11 min， 95% B； 11~17 min， 95~1% B'], 'SOLVENT_A': ['0.1% formic acid in water', 'formic acid in water'], 'SOLVENT_B': ['0.1% formic acid in methanol', 'formic acid in methanol']}
Sections "STUDY" contain missmatched keys: ['SUBMIT_DATE']
Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ['Mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum. Probiotic was fed at a dose of 1*10^9 CFU/ml/kg body weight in PBS solution by oral gavage (2 days/week, p.o., three weeks).', 'mice were randomly assigned into two experimental groups, named control and intervention groups, receiving standard chow feeding, and standard chow + L. plantarum']}
Sections "MS" contain missmatched items: {'INSTRUMENT_NAME': ['ABI Sciex 5000', 'ABI Sciex 5500 QTrap'], 'MS_COMMENTS': ['Ion Source Temperature 500°C, Ion Source Voltage 5500 V, Collision Gas 6 psi, Curtain Gas 30 psi, Atomizing Gas and Auxiliary Gas both 50 psi. 45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed.The raw data were firstly converted to mzXML format by MSConvert in the ProteoWizard software package (v3.0.8789) (Adusumilli et al., 2017) and processed using XCMS (Domingo-Almenara et al., 2020) for feature detection, retention time correction and alignment.', '45 arbitrary units (AU), 13 AU, 1 AU, 350 °C, and 350 °C for sheath gas, aux gas, sweep gas, ion transfer tube, and vaporizer temperature, respectively. The ion source was operated using heated ESI with an ion spray voltage set at 5500 V in positive ion mode. Polarity switching and scheduled selected reaction monitoring (SRM) were employed.MS Convert software (Proteowizard, v3.0.8789) was applied to convert “.raw” format raw data to “.mzML” format, which would be used for downstream analysis.']}
'Metabolites' section of 'MS_METABOLITE_DATA' block do not match.
'Data' section of 'MS_METABOLITE_DATA' block do not match.