Comparison Log
2025-12-15 02:41:21.156185
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006000/mwtab/...
Study ID:    ST003652
Analysis ID: AN006000
Status:      Inconsistent

Sections "PROJECT" contain missmatched items: {'PROJECT_SUMMARY': ["In chronic kidney disease (CKD) research, animal models provide invaluable insights into the disease’s etiopathogenesis and progression, particularly through the evaluation of renal tissue. The unilateral ureteral obstruction (UUO) rodent model stands out for its widespread use in CKD studies, due to its advantages to generate renal fibrosis and accelerated mimicry of obstructive nephropathy in humans. Despite its extensive use, the molecular underpinnings driving kidney disease progression remain incompletely understood. Given the crucial interplay between metabolism and fibrosis in CKD, a thorough examination of the UUO renal tissue through metabolomics is required. Untargeted multiplatform analysis enables a comprehensive measurement of the sample metabolic profile, ensuring a maximum coverage of metabolite diversity to yield extensive insights into the metabolism of this renal injury model. Therefore, in this study, murine kidney tissue from the UUO model underwent analysis using three separation techniques—liquid chromatography (LC), gas chromatography (GC), and capillary electrophoresis (CE)—coupled with mass spectrometry (MS). The findings reveal metabolic changes associated with tubulointerstitial fibrosis, impacting essential pathways such as the TCA cycle, urea cycle, polyamine metabolism, amino acids, one-carbon metabolism, purine catabolism, and NAD+ synthesis, among others. Furthermore, fibrosis significantly influences the renal tissue's lipidomic profile, characterized by a general decrease in most lipid classes and an increase in glycerophospholipids with ether substituents, hexosylceramides, and cholesterol esters compared to the control. These results underscore the relevance of the untargeted multiplatform approach to obtain a comprehensive overview of the alterations within the renal metabolic map, paving the way for further exploration of the molecular mechanisms underlying CKD.", "In chronic kidney disease (CKD) research, animal models provide invaluable insights into the disease’s etiopathogenesis and progression, particularly through the evaluation of renal tissue. The unilateral ureteral obstruction (UUO) rodent model stands out for its widespread use in CKD studies, due to its advantages to generate renal fibrosis and accelerated mimicry of obstructive nephropathy in humans. Despite its extensive use, the molecular underpinnings driving kidney disease progression remain incompletely understood. Given the crucial interplay between metabolism and fibrosis in CKD, a thorough examination of the UUO renal tissue through metabolomics is required. Untargeted multiplatform analysis enables a comprehensive measurement of the sample metabolic profile, ensuring a maximum coverage of metabolite diversity to yield extensive insights into the metabolism of this renal injury model. Therefore, in this study, murine kidney tissue from the UUO model underwent analysis using three separation techniques—liquid chromatography (LC), gas chromatography (GC), and capillary electrophoresis (CE)—coupled with mass spectrometry (MS). The findings reveal metabolic changes associated with tubulointerstitial fibrosis, impacting essential pathways such as the TCA cycle, urea cycle, polyamine metabolism, amino acids, one-carbon metabolism, purine catabolism, and NAD+ synthesis, among others. Furthermore, fibrosis significantly influences the renal tissue''s lipidomic profile, characterized by a general decrease in most lipid classes and an increase in glycerophospholipids with ether substituents, hexosylceramides, and cholesterol esters compared to the control. These results underscore the relevance of the untargeted multiplatform approach to obtain a comprehensive overview of the alterations within the renal metabolic map, paving the way for further exploration of the molecular mechanisms underlying CKD."]}
Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ['All animal-related procedures and sample collections were conducted at the Centro de Biología Molecular "Severo Ochoa" (CBMSO) in accordance with the guidelines outlined in Directive 2010/63/EU of the European Parliament, known as the Guide for the Care and Use of Laboratory Animals. The study (PROEX 098.0/22) received approval from the CBMSO Ethics Committee for Animal Experimentation, the ethics committee of the Consejo Superior de Investigaciones Científicas (CSIC) and the Regulatory Unit for Animal Experimental Procedures of the Comunidad de Madrid. Mice were housed in a specific pathogen-free animal facility at CBMSO, adhering to EU regulations.', 'All animal-related procedures and sample collections were conducted at the Centro de Biología Molecular Severo Ochoa (CBMSO) in accordance with the guidelines outlined in Directive 2010/63/EU of the European Parliament, known as the Guide for the Care and Use of Laboratory Animals. The study (PROEX 098.0/22) received approval from the CBMSO Ethics Committee for Animal Experimentation, the ethics committee of the Consejo Superior de Investigaciones Científicas (CSIC) and the Regulatory Unit for Animal Experimental Procedures of the Comunidad de Madrid. Mice were housed in a specific pathogen-free animal facility at CBMSO, adhering to EU regulations.']}
Sections "STUDY" contain missmatched items: {'STUDY_SUMMARY': ["In chronic kidney disease (CKD) research, animal models provide invaluable insights into the disease’s etiopathogenesis and progression, particularly through the evaluation of renal tissue. The unilateral ureteral obstruction (UUO) rodent model stands out for its widespread use in CKD studies, due to its advantages to generate renal fibrosis and accelerated mimicry of obstructive nephropathy in humans. Despite its extensive use, the molecular underpinnings driving kidney disease progression remain incompletely understood. Given the crucial interplay between metabolism and fibrosis in CKD, a thorough examination of the UUO renal tissue through metabolomics is required. Untargeted multiplatform analysis enables a comprehensive measurement of the sample metabolic profile, ensuring a maximum coverage of metabolite diversity to yield extensive insights into the metabolism of this renal injury model. Therefore, in this study, murine kidney tissue from the UUO model underwent analysis using three separation techniques—liquid chromatography (LC), gas chromatography (GC), and capillary electrophoresis (CE)—coupled with mass spectrometry (MS). The findings reveal metabolic changes associated with tubulointerstitial fibrosis, impacting essential pathways such as the TCA cycle, urea cycle, polyamine metabolism, amino acids, one-carbon metabolism, purine catabolism, and NAD+ synthesis, among others. Furthermore, fibrosis significantly influences the renal tissue's lipidomic profile, characterized by a general decrease in most lipid classes and an increase in glycerophospholipids with ether substituents, hexosylceramides, and cholesterol esters compared to the control. These results underscore the relevance of the untargeted multiplatform approach to obtain a comprehensive overview of the alterations within the renal metabolic map, paving the way for further exploration of the molecular mechanisms underlying CKD.", "In chronic kidney disease (CKD) research, animal models provide invaluable insights into the disease’s etiopathogenesis and progression, particularly through the evaluation of renal tissue. The unilateral ureteral obstruction (UUO) rodent model stands out for its widespread use in CKD studies, due to its advantages to generate renal fibrosis and accelerated mimicry of obstructive nephropathy in humans. Despite its extensive use, the molecular underpinnings driving kidney disease progression remain incompletely understood. Given the crucial interplay between metabolism and fibrosis in CKD, a thorough examination of the UUO renal tissue through metabolomics is required. Untargeted multiplatform analysis enables a comprehensive measurement of the sample metabolic profile, ensuring a maximum coverage of metabolite diversity to yield extensive insights into the metabolism of this renal injury model. Therefore, in this study, murine kidney tissue from the UUO model underwent analysis using three separation techniques—liquid chromatography (LC), gas chromatography (GC), and capillary electrophoresis (CE)—coupled with mass spectrometry (MS). The findings reveal metabolic changes associated with tubulointerstitial fibrosis, impacting essential pathways such as the TCA cycle, urea cycle, polyamine metabolism, amino acids, one-carbon metabolism, purine catabolism, and NAD+ synthesis, among others. Furthermore, fibrosis significantly influences the renal tissue''s lipidomic profile, characterized by a general decrease in most lipid classes and an increase in glycerophospholipids with ether substituents, hexosylceramides, and cholesterol esters compared to the control. These results underscore the relevance of the untargeted multiplatform approach to obtain a comprehensive overview of the alterations within the renal metabolic map, paving the way for further exploration of the molecular mechanisms underlying CKD."]}