Comparison Log
2025-12-15 02:56:37.598549
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006371/mwtab/...
Study ID:    ST003878
Analysis ID: AN006371
Status:      Inconsistent

Sections "PROJECT" contain missmatched items: {'ADDRESS': ["No. 4221 Xiang'an South Road, Xiang'an District, Xiamen City, Fujian Province, Xiamen, Fujian, 361100, China", "No. 4221 Xiang''an South Road, Xiang''an District, Xiamen City, Fujian Province, Xiamen, Fujian, 361100, China"]}
Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ["293T cells and 4T1 breast cancer cells were cultured in dulbecco's modified eagle medium (DMEM respectively, BasalMedia L120KJ) supplemented with 10% fetal bovine serum (FBS, VivaCell C04001-500) and 1% pen–strep antibiotic (Beyotime C0224-100ml). These cells were cultured at 37℃ in a humidified atmosphere containing 5% CO2.", "293T cells and 4T1 breast cancer cells were cultured in dulbecco''s modified eagle medium (DMEM respectively, BasalMedia L120KJ) supplemented with 10% fetal bovine serum (FBS, VivaCell C04001-500) and 1% pen–strep antibiotic (Beyotime C0224-100ml). These cells were cultured at 37℃ in a humidified atmosphere containing 5% CO2."]}
Sections "STUDY" contain missmatched items: {'ADDRESS': ["No. 4221 Xiang'an South Road, Xiang'an District, Xiamen City, Fujian Province, Xiamen, Fujian, 361100, China", "No. 4221 Xiang''an South Road, Xiang''an District, Xiamen City, Fujian Province, Xiamen, Fujian, 361100, China"]}
Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ["Plasmid/shRNA Construction and Virus Infection: Knockdown cell lines were generated by lentiviral infection. Briefly, 293T cells were co-transfected with these plasmids: psPAX2, pMD2.G and pLKO.1 (Hedgehog Bio Science and Technology Ltd. HH-shRNA-066) containing a shRNA sequence targeting PSAP mRNA. The following sequences were used: shPSAP 1: 5'- GAACTACGTGGACCAGTATTC-3'; shPSAP 2: 5'- GAATACTGGTCCACGTAGTTC-3'. The plasmids and shRNA were mixed in FBS-free DMEM with lipo 3000 kit (Invitrogen L3000015) to form a complex, and incubated with 293T cell line in FBS-free DMEM in 6-wells plates. The medium was changed with 10% FBS DMEM after 8h and co-infected in incubator for 48h. After co-infection, lentiviral supernatants were collected and mixed with virus concentration kit (Beyotime, C2901L). After 12000g centrifuging for 2min at 4℃, 4T1 cells were infected with shNC or shPSAP supernatants, as well as 10% FBS DMEM and 10μg/ml polybrene (Biosharp, BL628A) for 48 h. Stably PSAP knockdown 4T1 cells lines were selected with 2 μg/ml blasticidin.", "Plasmid/shRNA Construction and Virus Infection: Knockdown cell lines were generated by lentiviral infection. Briefly, 293T cells were co-transfected with these plasmids: psPAX2, pMD2.G and pLKO.1 (Hedgehog Bio Science and Technology Ltd. HH-shRNA-066) containing a shRNA sequence targeting PSAP mRNA. The following sequences were used: shPSAP 1: 5''- GAACTACGTGGACCAGTATTC-3''; shPSAP 2: 5''- GAATACTGGTCCACGTAGTTC-3''. The plasmids and shRNA were mixed in FBS-free DMEM with lipo 3000 kit (Invitrogen L3000015) to form a complex, and incubated with 293T cell line in FBS-free DMEM in 6-wells plates. The medium was changed with 10% FBS DMEM after 8h and co-infected in incubator for 48h. After co-infection, lentiviral supernatants were collected and mixed with virus concentration kit (Beyotime, C2901L). After 12000g centrifuging for 2min at 4℃, 4T1 cells were infected with shNC or shPSAP supernatants, as well as 10% FBS DMEM and 10μg/ml polybrene (Biosharp, BL628A) for 48 h. Stably PSAP knockdown 4T1 cells lines were selected with 2 μg/ml blasticidin."]}