Comparison Log
2025-12-15 03:08:23.208642
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006602/mwtab/...
Study ID:    ST004003
Analysis ID: AN006602
Status:      Inconsistent

Sections "SAMPLEPREP" contain missmatched items: {'SAMPLEPREP_SUMMARY': ['Before starting, calculate the volume of 1:1:1 choloroform:methanol:isopropanol resuspension buffer needed for each sample. Volume of 1:1:1 is dependent on normalize cell count from experiment. Use 200uL of 1:1:1 for every 2E5 cells. When ready to start resuspension, remove dried lipids from -80C storage and resuspend in volume of 1:1:1 solution calculated for each sample. No cell conditions use 200uL. Use gentle vortex to allow for dried lipids from vial wall to get into solution. Prepare "blank" vials of 1:1:1 for buffer background analysis. Store lipids in autosampler between 4-7C.', 'Before starting, calculate the volume of 1:1:1 choloroform:methanol:isopropanol resuspension buffer needed for each sample. Volume of 1:1:1 is dependent on normalize cell count from experiment. Use 200uL of 1:1:1 for every 2E5 cells. When ready to start resuspension, remove dried lipids from -80C storage and resuspend in volume of 1:1:1 solution calculated for each sample. No cell conditions use 200uL. Use gentle vortex to allow for dried lipids from vial wall to get into solution. Prepare blank vials of 1:1:1 for buffer background analysis. Store lipids in autosampler between 4-7C.']}
Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ["Cells were grown to full confluence and held for 3-days in Dulbecco's modified eagle medium (DMEM) with 10% fetal bovine serum (FBS). 24 hours prior to infection, cells were starved of serum in DMEM lacking FBS. Cells were HCMV-infected or mock-infected in serum-free DMEM for 1 hour. 1 hpi, 13C-choline isotopic tracer was fed to cells in serum-free growth medium we previously prepared (based on the published DMEM Gibco recipe). Tracer media contains 13C-choline in place 12C-choline. Tracer molecule was added at the same concentration non-tracer molecule is normally present in DMEM (13C-choline (4mg/L)). Medium was replaced at 48 hpi.", "Cells were grown to full confluence and held for 3-days in Dulbecco''s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS). 24 hours prior to infection, cells were starved of serum in DMEM lacking FBS. Cells were HCMV-infected or mock-infected in serum-free DMEM for 1 hour. 1 hpi, 13C-choline isotopic tracer was fed to cells in serum-free growth medium we previously prepared (based on the published DMEM Gibco recipe). Tracer media contains 13C-choline in place 12C-choline. Tracer molecule was added at the same concentration non-tracer molecule is normally present in DMEM (13C-choline (4mg/L)). Medium was replaced at 48 hpi."]}
'Data' section of 'MS_METABOLITE_DATA' block do not match.