Comparison Log
2025-12-15 03:08:27.618577
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN006603/mwtab/...
Study ID:    ST004004
Analysis ID: AN006603
Status:      Inconsistent

Sections "SAMPLEPREP" contain missmatched items: {'SAMPLEPREP_SUMMARY': ['Before starting, calculate the volume of 1:1:1 choloroform:methanol:isopropanol resuspension buffer needed for each sample. Volume of 1:1:1 is dependent on normalize cell count from experiment. Use 200uL of 1:1:1 for every 2E5 cells. When ready to start resuspension, remove dried lipids from -80C storage and resuspend in volume of 1:1:1 solution calculated for each sample. No cell conditions use 200uL. Use gentle vortex to allow for dried lipids from vial wall to get into solution. Prepare "blank" vials of 1:1:1 for buffer background analysis. Store lipids in autosampler between 4-7C.', 'Before starting, calculate the volume of 1:1:1 choloroform:methanol:isopropanol resuspension buffer needed for each sample. Volume of 1:1:1 is dependent on normalize cell count from experiment. Use 200uL of 1:1:1 for every 2E5 cells. When ready to start resuspension, remove dried lipids from -80C storage and resuspend in volume of 1:1:1 solution calculated for each sample. No cell conditions use 200uL. Use gentle vortex to allow for dried lipids from vial wall to get into solution. Prepare blank vials of 1:1:1 for buffer background analysis. Store lipids in autosampler between 4-7C.']}
Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ["Cells were grown to full confluence and held for 3-days in Dulbecco's modified eagle medium (DMEM) with 10% fetal bovine serum (FBS). 24 hours prior to infection, cells were starved of serum in DMEM lacking FBS. Cells were HCMV-infected or mock-infected in serum-free DMEM for 1 hour. The day of each experiment d5-LPC(17:0) was resuspended in EtOH at a concentration of 0.5%. Lipid-free BSA carrier protein (Sigma) was used at a final concentration of 1.7 µM to conjugate d5-LPC(17:0) prior to each experiment. 1 hpi, 10uM LPC(17:0)isotopic tracer or vehicle EtOH was fed to cells in serum-free growth medium. Tracer molecule and medium were replaced at 48 hpi.", "Cells were grown to full confluence and held for 3-days in Dulbecco''s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS). 24 hours prior to infection, cells were starved of serum in DMEM lacking FBS. Cells were HCMV-infected or mock-infected in serum-free DMEM for 1 hour. The day of each experiment d5-LPC(17:0) was resuspended in EtOH at a concentration of 0.5%. Lipid-free BSA carrier protein (Sigma) was used at a final concentration of 1.7 µM to conjugate d5-LPC(17:0) prior to each experiment. 1 hpi, 10uM LPC(17:0)isotopic tracer or vehicle EtOH was fed to cells in serum-free growth medium. Tracer molecule and medium were replaced at 48 hpi."]}
'Data' section of 'MS_METABOLITE_DATA' block do not match.