Comparison Log
2025-12-15 03:30:45.911310
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007273/mwtab/...
Study ID:    ST004356
Analysis ID: AN007273
Status:      Inconsistent

Sections "STUDY" contain missmatched items: {'STUDY_SUMMARY': ["Thus far, there is no standardized protocol using stable isotope tracing and gas chromatography mass spectrometry (GC-MS) in primary human immune cells. The goal of this study was to optimize the stable isotope tracing and subsequent GC-MS analysis of primary human peripheral blood mononuclear cells (PBMCs). We exposed human PBMCs to uniformly labeled 13C glucose, then extracted metabolites from each sample, separating polar metabolites from the protein pellet. Polar samples were dried and derivatized then analyzed via GCMS. Through this method, we found that incubating 10 million PBMCs per sample with 13C glucose or 13C glutamine for 2 hours provided optimal results, as this method allowed us to reliably detect the highest quantity of metabolites. We conclude that, when using a single-quadrupole GCMS to analyze a stable isotope i.e. 13C glucose's incorporation into metabolites in primary human PBMCs, a 2-hour incubation is recommended for optimal analysis.", "Thus far, there is no standardized protocol using stable isotope tracing and gas chromatography mass spectrometry (GC-MS) in primary human immune cells. The goal of this study was to optimize the stable isotope tracing and subsequent GC-MS analysis of primary human peripheral blood mononuclear cells (PBMCs). We exposed human PBMCs to uniformly labeled 13C glucose, then extracted metabolites from each sample, separating polar metabolites from the protein pellet. Polar samples were dried and derivatized then analyzed via GCMS. Through this method, we found that incubating 10 million PBMCs per sample with 13C glucose or 13C glutamine for 2 hours provided optimal results, as this method allowed us to reliably detect the highest quantity of metabolites. We conclude that, when using a single-quadrupole GCMS to analyze a stable isotope i.e. 13C glucose''s incorporation into metabolites in primary human PBMCs, a 2-hour incubation is recommended for optimal analysis."]}