Comparison Log
2026-01-11 07:05:58.661232
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007387/mwtab/...
Study ID:    ST003819
Analysis ID: AN007387
Status:      Inconsistent

Sections "SAMPLEPREP" contain missmatched items: {'SAMPLEPREP_SUMMARY': ["Cell pellet was resuspended in 400uL of 100% LC-MS grade methanol supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). with repeated pipetting up and down and vortexing for 10 seconds. Then, 100uL of LCMS-grade water containing 125 mM Ammonium Acetate, 10 mM Na-Ascorbate, and 7.9 mg/mL 5,5-dithio-bis-(2-nitrobenzoic acid (Ellman's reagent) was added, and the sample vortex for another 10 seconds. Samples were then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was transferred to a new tube and dried on ice using a liquid nitrogen dryer.", "Cell pellet was resuspended in 400uL of 100% LC-MS grade methanol supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). with repeated pipetting up and down and vortexing for 10 seconds. Then, 100uL of LCMS-grade water containing 125 mM Ammonium Acetate, 10 mM Na-Ascorbate, and 7.9 mg/mL 5,5-dithio-bis-(2-nitrobenzoic acid (Ellman''s reagent) was added, and the sample vortex for another 10 seconds. Samples were then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was transferred to a new tube and dried on ice using a liquid nitrogen dryer."]}
Sections "PROJECT" contain missmatched items: {'INSTITUTE': ["Boston Children's Hospital", "Boston Children''s Hospital"]}
Sections "STUDY" contain missmatched items: {'STUDY_SUMMARY': ['To investigate the effects of SLC31A1 knockout on steady state metabolite levels, SEM (acute lymphoblastic leukaemia cell line) cells containing either a SLC31A1-targeting sgRNA (two different guides, guide #2 or guide #4) or a intergenic -targetting sgRNA (i.e. a control sgRNA, "Intergenic1") and Cas9 were FACS-purified (Fluorescence Activated Cell Sorting) based on SLC31A1 expression. Cells were then cultured in RPMI-1640 containing 10% FBS and penicillin and streptomycin in 6-well plates, and then collected for metabolomics. SLC31A1 knockout cells were found to exhibit profound electron transport chain inhibition, resulting in accumulation of carbamoyl aspartic acid/ureidosuccinic acid, and depletion of aspartate, as well as broad changes in nucleotide mono, di and triphosphate levels.', 'To investigate the effects of SLC31A1 knockout on steady state metabolite levels, SEM (acute lymphoblastic leukaemia cell line) cells containing either a SLC31A1-targeting sgRNA (two different guides, guide #2 or guide #4) or a intergenic -targetting sgRNA (i.e. a control sgRNA, Intergenic1) and Cas9 were FACS-purified (Fluorescence Activated Cell Sorting) based on SLC31A1 expression. Cells were then cultured in RPMI-1640 containing 10% FBS and penicillin and streptomycin in 6-well plates, and then collected for metabolomics. SLC31A1 knockout cells were found to exhibit profound electron transport chain inhibition, resulting in accumulation of carbamoyl aspartic acid/ureidosuccinic acid, and depletion of aspartate, as well as broad changes in nucleotide mono, di and triphosphate levels.']}
'Metabolites' section of 'MS_METABOLITE_DATA' block do not match.