Comparison Log
2026-01-11 07:06:07.145140
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007474/mwtab/...
Study ID:    ST003822
Analysis ID: AN007474
Status:      Inconsistent

Sections "SAMPLEPREP" contain missmatched items: {'SAMPLEPREP_SUMMARY': ["Cell pellet was resuspended in 400uL of 100% LC-MS grade methanol supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). with repeated pipetting up and down and vortexing for 10 seconds. Then, 100uL of LCMS-grade water containing 125 mM Ammonium Acetate, 10 mM Na-Ascorbate, and 7.9 mg/mL 5,5-dithio-bis-(2-nitrobenzoic acid (Ellman's reagent) was added, and the sample vortex for another 10 seconds. Samples were then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was transferred to a new tube and dried on ice using a liquid nitrogen dryer. The dried metabolites were then resuspended in 30uL and 2uL was injected.", "Cell pellet was resuspended in 400uL of 100% LC-MS grade methanol supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). with repeated pipetting up and down and vortexing for 10 seconds. Then, 100uL of LCMS-grade water containing 125 mM Ammonium Acetate, 10 mM Na-Ascorbate, and 7.9 mg/mL 5,5-dithio-bis-(2-nitrobenzoic acid (Ellman''s reagent) was added, and the sample vortex for another 10 seconds. Samples were then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was transferred to a new tube and dried on ice using a liquid nitrogen dryer. The dried metabolites were then resuspended in 30uL and 2uL was injected."]}
Sections "PROJECT" contain missmatched items: {'INSTITUTE': ["Boston Children's Hospital", "Boston Children''s Hospital"]}
Sections "STUDY" contain missmatched items: {'STUDY_SUMMARY': ['To investigate whether the effects of SLC31A1 knockout can be rescued with alternative oxidase (AOX) overexpression, SEM cells containing a SLC31A1-targeting sgRNA (guide #2 or guide #7) or a intergenic -targetting sgRNA (i.e. a control sgRNA, "Intergenic1") and Cas9 were FACS-purified based on SLC31A1 expression, and then engineered to stably over-express either red fluorescent protein (RFP, as a control) or AOX from Ciona Intestinalis. Cells were then cultured in RPMI-1640 containing 10% FBS and penicillin and streptomycin in 6-well plates, and then collected for metabolomics. This demonstrated that the changes in aspartate, ureidosuccinic acid/carbamoyl aspartate and nucleotides were largely reverted by genetic overexpression of AOX.', 'To investigate whether the effects of SLC31A1 knockout can be rescued with alternative oxidase (AOX) overexpression, SEM cells containing a SLC31A1-targeting sgRNA (guide #2 or guide #7) or a intergenic -targetting sgRNA (i.e. a control sgRNA, Intergenic1) and Cas9 were FACS-purified based on SLC31A1 expression, and then engineered to stably over-express either red fluorescent protein (RFP, as a control) or AOX from Ciona Intestinalis. Cells were then cultured in RPMI-1640 containing 10% FBS and penicillin and streptomycin in 6-well plates, and then collected for metabolomics. This demonstrated that the changes in aspartate, ureidosuccinic acid/carbamoyl aspartate and nucleotides were largely reverted by genetic overexpression of AOX.']}
'Metabolites' section of 'MS_METABOLITE_DATA' block do not match.