Comparison Log
2026-01-11 07:51:23.097732
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007488/mwtab/...
Study ID:    ST004466
Analysis ID: AN007488
Status:      Inconsistent

Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ['Wild type and mlaA knockout E. coli were treated either with DMSO control, colistin, turnercyclamycin A, or turnercyclamycin B. MlaA is a protein that transports phospholipids in the Gram-negative bacterial outer membrane. In this work, we employed mlaA knockouts, sometimes referred to here as "mlaa" or "mlaako". In turn, the antibiotics are referred to as "col" (colistin), "tura" (turnercyclamycin A), and "turb" (turnercyclamycin B).', 'Wild type and mlaA knockout E. coli were treated either with DMSO control, colistin, turnercyclamycin A, or turnercyclamycin B. MlaA is a protein that transports phospholipids in the Gram-negative bacterial outer membrane. In this work, we employed mlaA knockouts, sometimes referred to here as mlaa or mlaako. In turn, the antibiotics are referred to as col (colistin), tura (turnercyclamycin A), and turb (turnercyclamycin B).']}
Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ['This study used compounds colistin, turnercyclamycin A, and turnercyclamycin B; and bacteria Escherichia coli BW25113 ("wild type") and Escherichia coli BW25113 ∆mlaA ("knockout"). Compound source and storage. Colistin was purchased from Thermo (J660915.03). Turnercyclamycins A and B were obtained by fermentation of Teredinibacter turnerae T7901 and purified to homogeneity using chemical and multistep chromatographic methods. The purity of compounds was assessed using LC coupled to diode array and high-resolution mass spec detectors, as well as by nuclear magnetic resonance experiments. Compounds were stored as dried powders at -20 C, while dilutions in DMSO were stored at -20 C. Stability of compounds was assessed by HPLC analysis, demonstrating long-term stability under storage conditions. Bacterial source and storage. Bacteria were obtained from the Keio Collection, stored as glycerol stocks at -80 C, and freshly plated the night before use in experiments. Cell growth and collection for phospholipidomics experiments. Growth of E. coli strains in the presence or absence of antibiotics was maintained for no more than 20 hours. Cells were pelleted by centrifiguation at the termination of the experiment and stored at -80 C until analysis.', 'This study used compounds colistin, turnercyclamycin A, and turnercyclamycin B; and bacteria Escherichia coli BW25113 (wild type) and Escherichia coli BW25113 ∆mlaA (knockout). Compound source and storage. Colistin was purchased from Thermo (J660915.03). Turnercyclamycins A and B were obtained by fermentation of Teredinibacter turnerae T7901 and purified to homogeneity using chemical and multistep chromatographic methods. The purity of compounds was assessed using LC coupled to diode array and high-resolution mass spec detectors, as well as by nuclear magnetic resonance experiments. Compounds were stored as dried powders at -20 C, while dilutions in DMSO were stored at -20 C. Stability of compounds was assessed by HPLC analysis, demonstrating long-term stability under storage conditions. Bacterial source and storage. Bacteria were obtained from the Keio Collection, stored as glycerol stocks at -80 C, and freshly plated the night before use in experiments. Cell growth and collection for phospholipidomics experiments. Growth of E. coli strains in the presence or absence of antibiotics was maintained for no more than 20 hours. Cells were pelleted by centrifiguation at the termination of the experiment and stored at -80 C until analysis.']}
'Metabolites' section of 'MS_METABOLITE_DATA' block do not match.