Comparison Log
2026-02-01 09:36:10.067218
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007500/mwtab/...
Study ID:    ST004474
Analysis ID: AN007500
Status:      Inconsistent

Sections "PROJECT" contain missmatched items: {'PROJECT_TITLE': ["AKT's acute regulation of glucose utilization in primary rat hepatocytes", "AKT''s acute regulation of glucose utilization in primary rat hepatocytes"]}
Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ["Primary rat hepatocytes were isolated from male Sprague Dawley rats between 150 and 175 g using the protocol described in PMID: 22781923 with a few modifications. Bovine serum albumin was added to William’s E complete medium (Gibco A1217601) to 1% w/v. To digest the liver, 20,000 units collagenase and 30 units elastase (Worthington CLSH LK002067) in addition to 1,000 Kunitz units of DNase (Worthington D2 LK003170) were dissolved in 250 mL Krebs-Ringer solution (Millipore Sigma #K4002) supplemented with 500 µM CaCl2 and 20 mM HEPES. All buffers and perfusion tubing were warmed to between 37 and 55°C to ensure adequate digestion. After digestion of the liver with all 250 mL of collagenase/elastase buffer, liver was dissected free and gently scraped in 150 mL of cold complete William’s E media. Cells were plated at a density of 175,000-500,000 cells per mL in 6-well tissue-culture treated plates coated with 1 mg/mL collagen in 0.2 N acetic acid. Plates were rinsed with PBS after collagen treatment and allowed to dry before plating cells. Hepatocytes were incubated in William's E complete medium (Gibco #A1217601) for four hours before incubating in serum and insulin-free DMEM lacking glucose, L-glutamine, Phenol Red or sodium pyruvate (Gibco #A1443001) overnight. After 16 hours in overnight medium, cells were pre-treated with inhibitors to start the experiment. After the experiment, media was collected and cells were scraped.", "Primary rat hepatocytes were isolated from male Sprague Dawley rats between 150 and 175 g using the protocol described in PMID: 22781923 with a few modifications. Bovine serum albumin was added to William’s E complete medium (Gibco A1217601) to 1% w/v. To digest the liver, 20,000 units collagenase and 30 units elastase (Worthington CLSH LK002067) in addition to 1,000 Kunitz units of DNase (Worthington D2 LK003170) were dissolved in 250 mL Krebs-Ringer solution (Millipore Sigma #K4002) supplemented with 500 µM CaCl2 and 20 mM HEPES. All buffers and perfusion tubing were warmed to between 37 and 55°C to ensure adequate digestion. After digestion of the liver with all 250 mL of collagenase/elastase buffer, liver was dissected free and gently scraped in 150 mL of cold complete William’s E media. Cells were plated at a density of 175,000-500,000 cells per mL in 6-well tissue-culture treated plates coated with 1 mg/mL collagen in 0.2 N acetic acid. Plates were rinsed with PBS after collagen treatment and allowed to dry before plating cells. Hepatocytes were incubated in William''s E complete medium (Gibco #A1217601) for four hours before incubating in serum and insulin-free DMEM lacking glucose, L-glutamine, Phenol Red or sodium pyruvate (Gibco #A1443001) overnight. After 16 hours in overnight medium, cells were pre-treated with inhibitors to start the experiment. After the experiment, media was collected and cells were scraped."]}
'Metabolites' section of 'MS_METABOLITE_DATA' block do not match.