Comparison Log
2026-02-22 08:09:29.699379
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007791/mwtab/...
Study ID:    ST004618
Analysis ID: AN007791
Status:      Inconsistent

Sections "TREATMENT" contain missmatched items: {'TREATMENT_SUMMARY': ["DMEM without glutamine, methionine, or cystine (Gibco, 21013024) was used for cystine and methionine deprivation. Dialyzed FSB (dFBS) was used to exclude the small amount of amino acids contained in the FBS. L-Methionine (Sigma, M5308) was dissolved in deionized distilled water, and L-cystine (Sigma, C7602) was dissolved in 1 N HCl (junsei, 20010S0350). L-Glutamine solution (200 mM; GibcoTM, 25030081) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The methionine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-cystine, 10% dFBS and 1% Anti-Anti. The cysteine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-methionine, 10% dFBS and 1% Anti-Anti. Cystine- and methionine-deficient media were composed of 4 mM L-glutamine, 10% dFBS and 1% Anti-Anti. The cells were washed twice with prewarmed Dulbecco's phosphate-buffered saline (DPBS) to remove amino acids contained in the growth medium. After replacement with conditioned medium, the cells were incubated in a 37°C incubator for 24 hours", "DMEM without glutamine, methionine, or cystine (Gibco, 21013024) was used for cystine and methionine deprivation. Dialyzed FSB (dFBS) was used to exclude the small amount of amino acids contained in the FBS. L-Methionine (Sigma, M5308) was dissolved in deionized distilled water, and L-cystine (Sigma, C7602) was dissolved in 1 N HCl (junsei, 20010S0350). L-Glutamine solution (200 mM; GibcoTM, 25030081) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The methionine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-cystine, 10% dFBS and 1% Anti-Anti. The cysteine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-methionine, 10% dFBS and 1% Anti-Anti. Cystine- and methionine-deficient media were composed of 4 mM L-glutamine, 10% dFBS and 1% Anti-Anti. The cells were washed twice with prewarmed Dulbecco''s phosphate-buffered saline (DPBS) to remove amino acids contained in the growth medium. After replacement with conditioned medium, the cells were incubated in a 37°C incubator for 24 hours"]}
Sections "STUDY" contain missmatched items: {'STUDY_SUMMARY': ["Cell culture NCI-H1299 and A549 were cultured in RPMI-1640 medium (Corning, 10-040-CVRC) supplemented with 10% fetal bovine serum (FBS; Thermo, 1009141) and 1% antibiotic–antimycotic (Anti-Anti; Gibco, 15240062). HepG2, Hep3B, HeLa and Hs746T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, SH30243.01) supplemented with 10% FBS and 1% Anti-Anti. NCI-H2009 cells were maintained in HyClone DMEM containing 5% FBS and 1% Anti-Anti. H9c2 cells were cultured in DMEM (Corning, 10-013-CVRC) supplemented with 10% FBS and 1% Anti-Anti. Unless otherwise specified, cells were maintained at 37°C in a humidified atmosphere containing 5% CO₂. Cystine and Methionine Deprivation DMEM without glutamine, methionine, or cystine (Gibco, 21013024) was used for cystine and methionine deprivation. Dialyzed FSB (dFBS) was used to exclude the small amount of amino acids contained in the FBS. L-Methionine (Sigma, M5308) was dissolved in deionized distilled water, and L-cystine (Sigma, C7602) was dissolved in 1 N HCl (junsei, 20010S0350). L-Glutamine solution (200 mM; GibcoTM, 25030081) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The methionine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-cystine, 10% dFBS and 1% Anti-Anti. The cysteine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-methionine, 10% dFBS and 1% Anti-Anti. Cystine- and methionine-deficient media were composed of 4 mM L-glutamine, 10% dFBS and 1% Anti-Anti. The cells were washed twice with prewarmed Dulbecco's phosphate-buffered saline (DPBS) to remove amino acids contained in the growth medium. After replacement with conditioned medium, the cells were incubated in a 37°C incubator for 24 hours. Collectively, these findings identify methionine as a critical metabolic coordinator of ferroptosis, acting through dynamic remodeling of amino acid, lipid, and redox metabolism, and reveal a previously underappreciated link between methionine availability and ferroptotic cell death.", "Cell culture NCI-H1299 and A549 were cultured in RPMI-1640 medium (Corning, 10-040-CVRC) supplemented with 10% fetal bovine serum (FBS; Thermo, 1009141) and 1% antibiotic–antimycotic (Anti-Anti; Gibco, 15240062). HepG2, Hep3B, HeLa and Hs746T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, SH30243.01) supplemented with 10% FBS and 1% Anti-Anti. NCI-H2009 cells were maintained in HyClone DMEM containing 5% FBS and 1% Anti-Anti. H9c2 cells were cultured in DMEM (Corning, 10-013-CVRC) supplemented with 10% FBS and 1% Anti-Anti. Unless otherwise specified, cells were maintained at 37°C in a humidified atmosphere containing 5% CO₂. Cystine and Methionine Deprivation DMEM without glutamine, methionine, or cystine (Gibco, 21013024) was used for cystine and methionine deprivation. Dialyzed FSB (dFBS) was used to exclude the small amount of amino acids contained in the FBS. L-Methionine (Sigma, M5308) was dissolved in deionized distilled water, and L-cystine (Sigma, C7602) was dissolved in 1 N HCl (junsei, 20010S0350). L-Glutamine solution (200 mM; GibcoTM, 25030081) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The methionine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-cystine, 10% dFBS and 1% Anti-Anti. The cysteine-deficient medium was composed of 4 mM L-glutamine, 0.2 mM L-methionine, 10% dFBS and 1% Anti-Anti. Cystine- and methionine-deficient media were composed of 4 mM L-glutamine, 10% dFBS and 1% Anti-Anti. The cells were washed twice with prewarmed Dulbecco''s phosphate-buffered saline (DPBS) to remove amino acids contained in the growth medium. After replacement with conditioned medium, the cells were incubated in a 37°C incubator for 24 hours. Collectively, these findings identify methionine as a critical metabolic coordinator of ferroptosis, acting through dynamic remodeling of amino acid, lipid, and redox metabolism, and reveal a previously underappreciated link between methionine availability and ferroptotic cell death."]}