Comparison Log
2026-03-15 08:19:10.235043
mwtab Python Library Version: 2.0.0
Source:      https://www.metabolomicsworkbench.org/rest/study/analysis_id/AN007839/mwtab/...
Study ID:    ST004646
Analysis ID: AN007839
Status:      Inconsistent

Sections "COLLECTION" contain missmatched items: {'COLLECTION_SUMMARY': ['MRC5-hT cells were grown to confluence in 6-well plates. Medium was replaced with serum-free DMEM for 24\u2009h prior to infection. At 48\u2009h post-infection, 2\u2009h before harvest, medium was replaced with serum-free DMEM pre-equilibrated at 37\u2009°C in a 5% CO₂ atmosphere. Plates were washed once with ice-cold PBS + glucose (Sigma G-8270; volume equal to previous medium), and 1\u2009mL dry ice–cooled extraction solution (80% MeOH, 20% Water) was added to each well. Cells were scraped, pipetted up and down three times, and transferred to 1.5\u2009mL tubes. Tubes were incubated for 15\u2009min on dry ice, then for 30\u2009min on ice, vortexing for 5\u2009s every 10\u2009min (three total vortex steps). Lysates were centrifuged at 14,000\u2009rpm (max speed) for 10\u2009min at 4\u2009°C. Supernatant (900\u2009µL) was collected, leaving ~100\u2009µL to avoid pellet disruption, dried under nitrogen, and stored at −80\u2009°C. ', 'MRC5-hT cells were grown to confluence in 6-well plates. Medium was replaced with serum-free DMEM for 24\u2009h prior to infection. At 48\u2009h post-infection, 2\u2009h before harvest, medium was replaced with serum-free DMEM pre-equilibrated at 37\u2009°C in a 5% CO₂ atmosphere. Plates were washed once with ice-cold PBS + glucose (Sigma G-8270; volume equal to previous medium), and 1\u2009mL dry ice–cooled extraction solution (80% MeOH, 20% Water) was added to each well. Cells were scraped, pipetted up and down three times, and transferred to 1.5\u2009mL tubes. Tubes were incubated for 15\u2009min on dry ice, then for 30\u2009min on ice, vortexing for 5\u2009s every 10\u2009min (three total vortex steps). Lysates were centrifuged at 14,000\u2009rpm (max speed) for 10\u2009min at 4\u2009°C. Supernatant (900\u2009µL) was collected, leaving ~100\u2009µL to avoid pellet disruption, dried under nitrogen, and stored at −80\u2009°C.']}