// Parameters
threshold1 = 49;
threshold2 = 100;
threshold3 = 10;

run("Close All");

// Code
open("https://github.com/NEUBIAS/training-resources/raw/master/image_data/xy_8bit__two_cells.tif");
rename("input");

// apply threshold 1
selectWindow("input");
run("Duplicate...", "title=threshold1");
setThreshold(threshold1, 65535);
setOption("BlackBackground", true);
run("Convert to Mask");

// apply threshold 2
selectWindow("input");
run("Duplicate...", "title=threshold2");
setThreshold(threshold2, 65535);
setOption("BlackBackground", true);
run("Convert to Mask");

// apply threshold 3
selectWindow("input");
run("Duplicate...", "title=threshold3");
setThreshold(threshold3, 65535);
setOption("BlackBackground", true);
run("Convert to Mask");