generated: '2026-08-23' method: searched source: >- https://github.com/ksqtx/TRACE and its published command reference https://github.com/ksqtx/TRACE/blob/main/CLI_commands.md, read on 2026-08-23. note: >- This is a first-party command-line tool published by KSQ Therapeutics in its own GitHub organization. It is NOT an API client — KSQ ships no API for a CLI to wrap. It is a bioinformatics analysis tool for single-cell RNA-seq classification, and it is recorded because it is real developer-facing software the company publishes, not because it exercises the content API catalogued elsewhere in this repo. name: trace binary: trace repository: https://github.com/ksqtx/TRACE docs: https://github.com/ksqtx/TRACE/blob/main/CLI_commands.md language: python framework: Click version: 0.1.0 license: GPL-3.0 (repository) / MIT (pyproject.toml) wraps_api: false install: - method: git command: pip install git+https://github.com/ksqtx/TRACE.git note: Not published to PyPI. requires-python >=3.11. global_options: - flag: --log-level values: - DEBUG - INFO - WARNING - ERROR - CRITICAL default: INFO - flag: --config description: Path to a YAML configuration file. - flag: --output-dir description: Output directory for results and artifacts. default: ./output commands: - group: meta commands: - name: version description: Display TRACEpy and Python version information. - name: init-config description: Generate a configuration template (default or batch). - name: validate-config description: Validate a configuration file's structure and values. - group: data commands: - name: preprocess description: >- Preprocess single-cell RNA-seq data from h5ad files — gene selection plus clone-level or cell-level expression summarisation for downstream ML. - name: chunk-remote-h5ad description: Chunk a remote h5ad dataset for processing. - group: model commands: - name: train description: Train a classifier on preprocessed expression data. - name: evaluate description: Evaluate a trained model. - name: compare description: Compare models. - name: retrain description: Retrain an existing model. - name: predict description: Run predictions with a trained model. - name: batch description: Run a batch workflow from a batch configuration. - group: analysis commands: - name: analyze-gene-significance description: Analyse gene-level significance in a trained model. - name: classify-cells description: Classify cells from single-cell input. command_count: 13