{
  "$schema": "http://json-schema.org/draft-07/schema#",
  "$id": "https://raw.githubusercontent.com/api-evangelist/labguru/main/json-schema/PrimerBaseRequest.json",
  "title": "PrimerBaseRequest",
  "type": "object",
  "required": [
    "token"
  ],
  "properties": {
    "token": {
      "type": "string",
      "example": "YOUR TOKEN IS HERE"
    },
    "item": {
      "type": "object",
      "properties": {
        "title": {
          "type": "string",
          "description": "The name of the primer"
        },
        "alternative_name": {
          "type": "string",
          "description": "Any alternative name by which the primer may be known"
        },
        "owner_id": {
          "type": "integer",
          "description": "id of the owner - if omitted, will default to your member id"
        },
        "sequence": {
          "type": "string",
          "description": "The nucleotide sequence of the primer"
        },
        "tag": {
          "type": "string",
          "description": "A unique label or tag associated with the primer"
        },
        "gene_id": {
          "type": "integer",
          "nullable": true,
          "description": "A reference ID linking the primer to a specific gene in your lab"
        },
        "tm": {
          "type": "string",
          "description": "The melting temperature (Tm) of the primer",
          "example": 3
        },
        "orientation": {
          "type": "string",
          "description": "Indicates the orientation of the primer. Set \"1\" for forward and \"0\" for reverse. This field is optional and can be left null if orientation is not applicable",
          "nullable": true,
          "example": "1"
        },
        "target_position": {
          "type": "string",
          "description": "The specific binding position of the primer on the target DNA"
        },
        "fragment_size": {
          "type": "string",
          "description": "The size of the DNA fragment that the primer is designed to amplify"
        },
        "organism": {
          "type": "string",
          "description": "The specific organism for which the primer is designed, such as \"Mouse\""
        },
        "used_for": {
          "type": "string",
          "description": "The specific applications for which the primer is used (pcr / RT / SDM / etc.)"
        },
        "restriction_site": {
          "type": "string",
          "description": "Information about any restriction enzyme recognition sites incorporated into the primer design"
        },
        "source": {
          "type": "string",
          "description": "The origin or source from which the primer was obtained"
        },
        "description": {
          "type": "string",
          "description": "A detailed description of the primer"
        }
      }
    }
  }
}