Structural basis of CRISPR-SpCas9 inhibition by an anti-CRISPR protein
CRISPR-Cas systems are nucleoprotein complexes that provide adaptive immunity against mobile genetic elements in prokaryotes. As a result of the widespread use of Cas9 in genome engineering, anti-CRISPR proteins that inhibit Cas9 have attracted interest as tools to control CRISPR-based genome editing. Here we report the structures of Staphylococcus aureus Cas9 (SaCas9) in complex with the anti-CRISPR protein AcrIIA4.
Department of Structural Biology, University of California, San Francisco, CA, USA
Howard Hughes Medical Institute, San Francisco, CA, USA
National Institute of General Medical Sciences