{
  "@context": "https://raw.githubusercontent.com/api-evangelist/pubmed/main/json-ld/pubmed-context.jsonld",
  "@type": "PubMedArticle",
  "@id": "https://pubmed.ncbi.nlm.nih.gov/36328499/",
  "pmid": "36328499",
  "pmcid": "PMC9876543",
  "doi": "10.1038/s41586-022-05543-x",
  "title": "Structural basis of CRISPR-SpCas9 inhibition by an anti-CRISPR protein",
  "abstract": "CRISPR-Cas systems are nucleoprotein complexes that provide adaptive immunity against mobile genetic elements in prokaryotes. As a result of the widespread use of Cas9 in genome engineering, anti-CRISPR proteins that inhibit Cas9 have attracted interest as tools to control CRISPR-based genome editing. Here we report the structures of Staphylococcus aureus Cas9 (SaCas9) in complex with the anti-CRISPR protein AcrIIA4.",
  "pubDate": "2022/12/15",
  "language": "eng",
  "authors": [
    {
      "@type": "Author",
      "lastName": "Smith",
      "firstName": "Jane",
      "initials": "J",
      "affiliation": "Department of Structural Biology, University of California, San Francisco, CA, USA",
      "orcid": "https://orcid.org/0000-0001-2345-6789"
    },
    {
      "@type": "Author",
      "lastName": "Jones",
      "firstName": "Michael",
      "initials": "M",
      "affiliation": "Howard Hughes Medical Institute, San Francisco, CA, USA"
    }
  ],
  "journal": {
    "@type": "Journal",
    "title": "Nature",
    "isoAbbreviation": "Nature",
    "issn": "0028-0836",
    "volume": "612",
    "issue": "7941",
    "pages": "456-463"
  },
  "meshTerms": [
    {
      "@type": "MeshTerm",
      "descriptorName": "CRISPR-Cas Systems",
      "descriptorUI": "D000071557",
      "isMajorTopic": true
    },
    {
      "@type": "MeshTerm",
      "descriptorName": "Gene Editing",
      "descriptorUI": "D000072669",
      "isMajorTopic": true
    },
    {
      "@type": "MeshTerm",
      "descriptorName": "Bacterial Proteins",
      "descriptorUI": "D001426",
      "isMajorTopic": false
    }
  ],
  "keywords": ["CRISPR", "anti-CRISPR", "Cas9", "genome editing", "structural biology"],
  "publicationTypes": ["Journal Article", "Research Support, N.I.H., Extramural"],
  "fullTextLinks": [
    {
      "url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876543/",
      "name": "PubMed Central",
      "category": "Free full text"
    },
    {
      "url": "https://doi.org/10.1038/s41586-022-05543-x",
      "name": "Nature",
      "category": "Full text"
    }
  ],
  "grantList": [
    {
      "@type": "Grant",
      "grantId": "R01 GM123456",
      "agency": "National Institute of General Medical Sciences",
      "acronym": "NIGMS",
      "country": "United States"
    },
    {
      "@type": "Grant",
      "grantId": "Howard Hughes Medical Institute",
      "agency": "Howard Hughes Medical Institute",
      "acronym": "HHMI",
      "country": "United States"
    }
  ],
  "citations": 127
}