Structural basis of CRISPR-SpCas9 inhibition by an anti-CRISPR protein
type: PubMedArticle
pmid: 36328499
pmcid: PMC9876543
doi: 10.1038/s41586-022-05543-x
abstract: CRISPR-Cas systems are nucleoprotein complexes that provide adaptive immunity against mobile genetic elements in prokaryotes. As a result of the widespread use of Cas9 in genome engineering, anti-CRISPR proteins that inhibit Cas9 have attracted interest as tools to control CRISPR-based genome editing. Here we report the structures of Staphylococcus aureus Cas9 (SaCas9) in complex with the anti-CRISPR protein AcrIIA4.
pub date: 2022/12/15
language: eng
authors: Author
  last name: Smith
  first name: Jane
  initials: J
  affiliation: Department of Structural Biology, University of California, San Francisco, CA, USA
  orcid: https://orcid.org/0000-0001-2345-6789
authors: Author
  last name: Jones
  first name: Michael
  initials: M
  affiliation: Howard Hughes Medical Institute, San Francisco, CA, USA
journal: Nature
  type: Journal
  iso abbreviation: Nature
  issn: 0028-0836
  volume: 612
  issue: 7941
  pages: 456-463
mesh terms: MeshTerm
  descriptor name: CRISPR-Cas Systems
  descriptor UI: D000071557
  is major topic: true
mesh terms: MeshTerm
  descriptor name: Gene Editing
  descriptor UI: D000072669
  is major topic: true
mesh terms: MeshTerm
  descriptor name: Bacterial Proteins
  descriptor UI: D001426
  is major topic: false
keywords: CRISPR
keywords: anti-CRISPR
keywords: Cas9
keywords: genome editing
keywords: structural biology
publication types: Journal Article
publication types: Research Support, N.I.H., Extramural
full text links:
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9876543/
  name: PubMed Central
  category: Free full text
full text links:
  url: https://doi.org/10.1038/s41586-022-05543-x
  name: Nature
  category: Full text
grant list: Grant
  grant id: R01 GM123456
  agency: National Institute of General Medical Sciences
  acronym: NIGMS
  country: United States
grant list: Grant
  grant id: Howard Hughes Medical Institute
  agency: Howard Hughes Medical Institute
  acronym: HHMI
  country: United States
citations: 127