aid: reactome
name: Reactome
description: >-
  Reactome is a free, open-source, curated, and peer-reviewed biological pathway
  database. It provides comprehensive pathway data covering human biology and
  orthologous reactions for over 14 non-human species including mouse, rat,
  yeast, and fruit fly. The platform exposes two primary REST APIs — the Content
  Service for querying pathway data, molecular interactions, species comparisons,
  and reaction network visualization, and the Analysis Service for pathway
  enrichment analysis of gene and protein identifier lists. Both APIs are freely
  accessible without authentication, fully documented with OpenAPI (Swagger), and
  cross-referenced with major biological databases including NCBI, Ensembl,
  UniProt, KEGG, ChEBI, PubMed, and Gene Ontology. Data is authored by expert
  biologist researchers and maintained by Reactome editorial staff.
type: Index
position: Provider
access: Public
image: https://kinlane-productions.s3.amazonaws.com/apis-json/apis-json-logo.jpg
tags:
  - Biological Pathways
  - Bioinformatics
  - Life Sciences
  - Pathway Analysis
  - Gene Enrichment
  - Molecular Interactions
  - Systems Biology
  - Open Science
url: >-
  https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/apis.yml
created: '2026-06-13'
modified: '2026-06-13'
specificationVersion: '0.19'
apis:
  - aid: reactome:content-service
    name: Reactome Content Service API
    description: >-
      The Reactome Content Service provides programmatic access to all
      knowledgebase content including pathways, reactions, physical entities,
      complexes, and molecular interactions. It supports querying by
      identifier, retrieving pathway hierarchies, exporting pathway diagrams
      in multiple formats (PNG, JPG, SVG, PDF, SBML, SBGN), searching via
      Solr full-text search, querying PSICQUIC interactor databases, and
      mapping external identifiers to Reactome entities. The API covers human
      biology as well as orthologous reactions for over 14 species. No
      authentication is required and the API is free to use for research
      purposes.
    humanURL: https://reactome.org/dev/content-service
    baseURL: https://reactome.org/ContentService
    tags:
      - Pathways
      - Reactions
      - Molecular Interactions
      - Diagram Export
      - Species Orthology
      - Search
      - Interactors
    properties:
      - type: Documentation
        url: https://reactome.org/dev/content-service
      - type: OpenAPI
        url: https://reactome.org/ContentService/v3/api-docs
      - type: OpenAPI
        url: >-
          https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/openapi/content-service.json
      - type: SwaggerUI
        url: https://reactome.org/ContentService/
  - aid: reactome:analysis-service
    name: Reactome Analysis Service API
    description: >-
      The Reactome Analysis Service enables pathway enrichment analysis for
      lists of gene or protein identifiers. Users can submit identifier lists
      via direct POST, file upload, or URL reference. The service
      automatically detects overrepresentation analysis for identifier lists
      or expression analysis for tab-separated values with numeric expression
      data. Results include pathway hit data, p-values, false discovery rate
      (FDR), and entity and reaction counts. Analysis results are assigned a
      token valid for 7 days, allowing subsequent retrieval, filtering by
      species, pagination, and PDF report generation without resubmitting
      data. Species comparison against Homo sapiens is also supported.
    humanURL: https://reactome.org/dev/analysis
    baseURL: https://reactome.org/AnalysisService
    tags:
      - Pathway Enrichment
      - Overrepresentation Analysis
      - Expression Analysis
      - Species Comparison
      - Gene Lists
      - Protein Identifiers
    properties:
      - type: Documentation
        url: https://reactome.org/dev/analysis
      - type: OpenAPI
        url: https://reactome.org/AnalysisService/v3/api-docs
      - type: OpenAPI
        url: >-
          https://raw.githubusercontent.com/api-evangelist/reactome/refs/heads/main/openapi/analysis-service.json
      - type: SwaggerUI
        url: https://reactome.org/AnalysisService/
common:
  - type: Website
    url: https://reactome.org/
  - type: Documentation
    url: https://reactome.org/documentation
  - type: DeveloperPortal
    url: https://reactome.org/dev
  - type: DataModel
    url: https://reactome.org/documentation/data-model
  - type: FAQ
    url: https://reactome.org/documentation/faq
  - type: GitHub
    url: https://github.com/reactome
  - type: Blog
    url: https://reactome.org/about/news
  - type: License
    url: https://reactome.org/license
  - type: Contact
    url: https://reactome.org/about/contact-us
  - type: About
    url: https://reactome.org/about
maintainers:
  - FN: Kin Lane
    email: kin@apievangelist.com