competitor1,competitor2,competitor1_initial_count,competitor2_initial_count,competitor1_final_count,competitor2_final_count,transfer_dilution,num_transfers,initial_dilution,initial_volume,final_dilution,final_volume
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
[ENTER NAME],[ENTER NAME],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],[ENTER COUNTS],100,1,1,1,1,1
# INSTRUCTIONS
# Fill in cells containing placeholders in [BRACKETS]. Change competition assay parameters from their default values as needed and remove/add lines as necessary
# Each line should have the results for one competition assay replicate. Repeat the names of competitor1 and competitor2 for all replicates of the same competition.
# COLUMN DESCRIPTIONS
# competitor1 = the name of competitor 1 (e.g. strain or sample designation).
## Example: REL606
# competitor2 = the name of competitor 2 (e.g. strain or sample designation).
## Example: REL8604
# competitor1_initial_count = the counts of competitor1 cells when the two competitors are mixed (e.g. colony counts).
## Example: 243
# competitor2_initial_count = the counts of competitor2 cells when the two competitors are mixed (e.g. colony counts).
## Example: 128
# competitor1_final_count = the counts of competitor1 cells after the competitors are co-cultured (e.g. colony counts).
## Example: 198
# competitor2_final_count = the counts of competitor 2 cells after the competitors are co-cultured (e.g. colony counts).
## Example: 271
# transfer_dilution = the daily dilution factor for all passages of the cells in co-culture. This should be the same as the overall dilution when they are first mixed.
## Example: 100  (If 2 x 50 µl of saturated cultures of each competitor were mixed into 9.9 mL of fresh medium and then the culture grew to saturation.)
# num_transfers = the number of transfers in competition experiment.
## Example: 3  (If 2 x 50 µl of saturated cultures of each competitor were mixed into 9.9 mL of fresh medium; then transferred into fresh medium on the following two days; and plated on the third day.)
# NOTE: For the following columns; if you used the same dilution procedure and analyzed the same volume of sample at the initial and final timepoints then you can leave the remaining columns as the DEFAULT values of 1 or delete them.
# initial_dilution = the dilution factor for the initial sample that was plated. Include any dilution that occurred when mixing the competitors together.
## Example: 10000  (If 2 x 50 µl of saturated cultures of each competitor were mixed into 9.9 mL of fresh medium; then 100 µl of this was diluted into 9.9 ml of sterile saline.)
# initial_volume = volume of the initial dilution that was analyzed for counting each competitor.  The units on initial_volume and final_volume must be the same.
## Example: 80  (If 80 µL were plated.)
# final_dilution – the dilution factor for the initial sample that was plated. Example: 10000 if 100 µl of final saturated cultures was diluted into 9.9 mL of sterile saline; then 100 µl of this was diluted into 9.9 ml of sterile saline.
## Example: 10000  (If 100 µl of final saturated cultures was diluted into 9.9 mL of sterile saline; then 100 µl of this was diluted into 9.9 ml of sterile saline.)
# final_volume – volume of the initial dilution that was analyzed for counting each competitor. The units on initial_volume and final_volume must be the same.
## Example: 80  (If 80 µL were plated.)