{ "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "### extract allelic raed counts from TCGA RNAseq and WXS bams\n", "\n", "| Description | Location | \n", "| ------------- | :------|\n", "|identify relevant TCGA WXS and RNAseq|http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/identify_TCGA_WGS_runs.ipynb |\n", "| | |" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# allelic fraction extraction in TCGA pipeline\n", "\n", "| input |output| Location | \n", "| ------------- |:-------------:| \n", "| raw bams from TCGA website |allelic read counts| http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/TestPipeline/submitJobForAllleleExtraction.ipynb |\n", "|TCGA website| preprocessed TCGA VCF files| http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/downloadingVCFs.ipynb|\n", "|TCGA website| VCF metadata annotations| http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/Pull_TCGA_meta_data.ipynb|\n", "|preprocessed TCGA VCF files| merged VCF file| http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/mergeTCGA_VCF.ipynb#|\n", "|reprocessed bam-reacount out files| merged bam-reacount out file|http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/TestPipeline/mergeBamReadCountFiles.ipynb\n", "\n", "# analysis\n", "| Description| Location | \n", "| ------------- |:-------------:| \n", "|calling mutation in TCGA using simple allelic read counts with less than 10 lines|http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/TestPipeline/mutationCalling.ipynb#|\n", "|analyze RNA-seq and WXS allelic read count concordance | http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/Compare_TCGA_RNAseq_WXS.ipynb |\n", "\n", "The URL locations are hardcoded for me clicking to go to different notebooks. \n" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "# scratch\n", "\n", "Find TCGA WXS runs\n", "http://localhost:6001/notebooks/Project/METAMAP/notebook/RapMapTest/XGS_WGS/identify_TCGA_WGS_runs.ipynb\n", "\n", "check for TCGA aligning speed: \n", "\n", "http://localhost:6001/edit/Project/METAMAP/notebook/RapMapTest/XGS_WGS/TestPipeline/align_tcga.py" ] }, { "cell_type": "code", "execution_count": null, "metadata": {}, "outputs": [], "source": [] } ], "metadata": { "kernelspec": { "display_name": "Python 3", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.6.5" } }, "nbformat": 4, "nbformat_minor": 2 }