Lead Creator: Carrine E. Blank, University of Montana, fall 2013 through spring 2016. Co-contributors and advisors: Hong Cui (University of Arizona), Lisa Moore (University of Southern Maine), and Ramona Walls (University of Arizona).
MicrO (An Ontology of Prokaryotic Phenotypic and Metabolic Characters). Version 1.5.1 released 6/14/2018. Includes terms and term synonyms extracted from > 3000 prokaryotic taxonomic descriptions, collected from a large number of taxonomic descriptions from Archaea, Cyanobacteria, Bacteroidetes, Firmicutes and Mollicutes. The ontology and the synonym lists were developed to facilitate the automated extraction of phenotypic data and character states from prokaryotic taxonomic descriptions using a natural language processing algorithm (MicroPIE). MicroPIE was developed by Hong Cui, Elvis Hsin-Hui Wu, and Jin Mao (University of Arizona) in collaboration with Carrine E. Blank (University of Montana) and Lisa R. Moore (University of Southern Maine). Descriptions and links to MicroPIE can be found at http://avatol.org/ngp/nlp/overview-2/. https://github.com/biosemantics/micropie2 The most current version of MicrO can be downloaded from https://github.com/carrineblank/MicrO.
Many thanks to Chris Mungall (LBNL), Elvis Hsin-Hui Wu (University of Arizona), Gail Gasparich (Towson University), and Gordon Burleigh (University of Florida) for comments and/or assistance with ontology construction and compilation of taxonomic descriptions and term definitions. Thanks to Oliver He (University of Michigan) for technical assistance with OntoBee and OntoFox, and Gareth Owen (ChEBI project leader, head curator) and other curators at ChEBI for assistance in the incorporation of microbial-specific chemical terms and synonyms into ChEBI. Thanks also to the instructors (Melissa Haendel, Matt Yoder, Jim Baihoff) and students of the 2013 NESCent Ontologies for Evolutionary Biology workshop, and to Karen Cranston (NESCent director) and the support staff at NESCent. This work was supported by a grant from the National Science Foundation Assembling the Tree of Life Program (DBI-1208534), and by a travel grant to attend the 2013 NESCent Ontologies for Evolutionary Biology workshop.
Relationship between an assay (x) and a microbiological culture medium (y), where the medium is needed to carry out the assay.
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a shape (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a position quality (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a structure (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a morphology (y).
Relationship between two connected entities that are connected and have a spatial relationship defined by an angle that exists between the two connected entities.
Relationship between an assay (x) and a chemical entity (y), where the chemical entity is used as a chemical reagent or substance used to carry out the assay.
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a size (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a physical quality (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a texture (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and an organismal quality (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a physical object quality (y).
is an enzymatic hydrolytic extract derived from
Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of enzymatic hydrolysis.
is an acid hydrolytic extract derived from
Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of acid hydrolysis.
Relationship between a quality (x) and an assay (y).
Relationship between a microbiological culture medium (x) and a cellular organism (y) where the culture medium is used to support the growth of cellular organism y.
Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical or physical extraction procedure.
Relationship between an oleoginous (oily) material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed from material derived from the organism.
Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is created as a result of a chemical extraction procedure performed via the process of an aqueous extraction (an extraction with water).
Relationship between an assay (x) and a quality (y).
Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y).
Relationship between an assay (x) and a chemical entity (y), where the chemical entity used is a chemical enzymatic reactant in the designated process (namely a chemical reaction catalyzed by an enzyme).
Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a chemical enzymatic product of the designated process (namely a chemical reaction catalyzed by an enzyme).
Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a metabolic substrate used in the designated process (generally a metabolic process deriving from a combination of enzymatic processes).
Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a metabolic product in the designated process (generally a metabolic process deriving from a combination of enzymatic processes).
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a metabolic substrate (generally a metabolic process deriving from a combination of enzymatic processes) assayed by the diagnostic assay.
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a chemical enzymatic reactant (namely a chemical reaction catalyzed by an enzyme) being assayed in the diagnostic assay.
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a metabolic product of the process (a metabolic process deriving from a combination of enzymatic processes) which is being assayed by the diagnostic assay.
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a chemical enzymatic product of the enzymatic process (a chemical reaction catalyzed by an enzyme) assayed by the diagnostic assay.
A relationship between a molecular function (x), namely the enzymatic activity being tested and an assay (y), namely an analytical investigation/test for the presence of an enzymatic activity.
is an assay for the enzymatic activity of
A relationship between an assay (x), namely an analytical investigation/test for the presence of an enzymatic activity, and a molecular function (y), namely the enzymatic activity being tested.
is an assay for the fermentation substrate
Relationship between an assay (x) and a chemical entity (y), where the chemical entity used is a fermentation substrate in the designated process (generally a metabolic fermentation process deriving from a combination of enzymatic processes).
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a fermentation product (generally a metabolic fermentation process deriving from a combination of enzymatic processes) assayed by the diagnostic assay.
Relationship between a chemical entity (x) and an assay (y), where the chemical entity is a fermentation substrate (generally a metabolic fermentation process deriving from a combination of enzymatic processes) being assayed by the diagnostic assay.
A relationship between a process, quality, cellular component, molecular function, or a chemical entity (x) and an assay (y).
A relationship between a microbiological diagnostic test (x) and a biological process, quality, cellular component, molecular function, or a chemical entity (y).
is an assay for the biological process of
A relationship between an assay (x), namely an analytical investigation/test for the presence of a physical biological process, and a biological process (y).
A relationship between a biological process (x), and an assay (y) which is an analytical investigation/test for the presence of a physical biological process.
is an assay for the biosynthetic production of
A relationship between a n assay (x), namely an analytical investigation/test for the presence of a metabolic product, and a chemical entity (y).
A relationship between a chemical entity (x), which is a biosynthetic product, that is assayed by an assay (y). Differentiated from 'is metabolic production of' which has the same domain and ranges, but is where an assay is used to detect a product of metabolism (i.e. energy generation).
A relationship between a prokaryotic physiological quality (x) and a prokaryotic physiologically differentiated cell (y).
A relationship between an assay (x), namely an analytical investigation/test for the presence of a physical cellular component, and a cellular component (y).
A relationship between a cellular component (x), and an assay (y) which is an analytical investigation/test for the presence of a cellular component.
Relationship between a material entity (x), which is a man-made material entity such as a chemical solution or a culture medium, and a chemical entity (y), where the chemical entity forms part of a mixture of chemicals that make up the material entity.
Wikipedia:ingredient An ingredient is a substance that forms part of a mixture (in a general sense). For example, in cooking, recipes specify which ingredients are used to prepare a specific dish. Many commercial products contain a secret ingredient that is purported to make them better than competing products. In the pharmaceutical industry, an active ingredient is that part of a formulation that yields the effect required by the customer. National laws usually require prepared food products to display a list of ingredients, and specifically require that certain additives be listed. In most developed countries, the law requires that ingredients be listed according to their relative weight[1] in the product. If an ingredient itself consists of more than one ingredient (such as the cookie pieces which are a part of "cookies and cream" flavor ice cream), then that ingredient is listed by what percentage of the total product it occupies, with its own ingredients displayed next to it in brackets. The term constituent is often chosen when referring to the substances that constitute the tissue of living beings such as plants and people, because the word ingredient in many minds connotes a sense of human agency (that is, something that a person combines with other substances), whereas the natural products present in living beings were not added by any human agency but rather occurred naturally ("a plant doesn't have ingredients"). Thus all ingredients are constituents, but not all constituents are ingredients.
Relationship between a chemical entity (x) and a mixture, culture medium, or chemical solution (y), where y is artificial created by human activity.
Relationship between a prokaryotic cell or cellular component (x) and a chemical solution or chemical entity (y).
Relationship between an independent continuant (x) and another independent continuant (y), where x develops (or transforms) into y through a developmental process.
Relationship between a microbiological culture medium (x) and a salinity quality (y).
Relationship between a microbiological culture medium (x) and a pH quality (y).
Relationship between a microbiological culture medium (x) and a redox quality (y).
Relationship between a microbiological culture medium (x) and a quality (y).
Relationship between a material entity (x) and a material entity (y), where the material entity is a natural substance (such as a chemical entity) that has a mixture of chemical substances in it.
Relationship between an assay (x) and a material entity (y), where the material entity is needed to carry out the assay.
Relationship between a quality (x), such as a pH quality, redox quality, or salinity quality, and a microbiological culture medium (y) which is an independent continuant.
Relationship between a material entity (x) and a cellular organism or environmental material (y) where the material entity is derived from the starting complex entity y.
Relationship between a particular cellular organism (x) and an external encapuslating structure (y).
Relationship between an assay (x) and cellular organisms (y), where the organism(s) is needed to carry out the assay.
Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is a carbon source.
Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity or sunlight (y), where the chemical entity or sunlight is a energy source.
Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is a electron donor.
Relationship between a prokaryotic metabolically differentiated cell or prokaryotic metabolic process (x) and a chemical entity (y), where the chemical entity is an electron acceptor.
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a symmetry quality (y).
Relationship between a cellular component, prokaryotic cell or prokaryotic colony (x) and a spatial position quality (y).
Relationship between a prokaryotic metabolically differentiated cell (x) and a prokaryotic metabolic process (y).
A relationship between a prokaryotic physiologically differentiated cell (x) and a prokaryotic physiological quality (y).
is prokaryotic metabolic process carried out in the presence of
A relationship between a prokaryotic metabolic process (x) and an inorganic molecular entity, an organic molecular entity or sunlight (y).
is prokaryotic metabolic process occuring in
Relationship between a prokaryotic metabolic process (x) and a prokaryotic metabolically differentiated cell (y).
Relationship between a prokaryotic metabolically differentiated cell (x) and a prokaryotic metabolic quality (y).
Relationship between a prokaryotic metabolic quality (x) and a prokaryotic metabolically differentiated cell (y) which is an independent continuant.
is prokaryotic metabolic quality of a cell carrying out the process
A relationship between a prokaryotic metabolic quality (x) and a prokaryotic metabolic process (y).
is prokaryotic metabolic process which makes a cell
Relationship between a prokaryotic metabolic process (x) and a prokaryotic metabolic quality (y).
Relationship between a prokaryotic colony (x) and a symmetry quality (y), pertaining specifically to the symmetry of the prokaryotic colony margin.
A relationship between a prokaryotic differentiated cell (x) and a biological process (y).
is an assay for the fermentation product
Relationship between an assay (x) and a chemical entity (y), where the chemical entity is a fermentation product in the designated process (generally a metabolic fermentation process deriving from a combination of enzymatic processes).
Describes the percentage of sodium chloride concentration in which an organism is capable of growth.
Describes the pH (hydrogen ion concentration) in which an organism is capable of growth.
zzzz Note that there is an error with this class (sulfate can't be used as an electron donor)
flagellar quality
Prokaryotic cell quality where a cell has either a bacterial-type flagellum (or flagella) or a periplasmic flagellum.
branch perpendicular to trichome
Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is perpendicular.
branches perpendicular to the long trichome axis
filament branch size
Filament branch morphological quality relating to the size (length, and hence the number of cells in the branch, and thickness) of a filament branch, relative to the size of the main trichome.
morphologically differentiated branches
Filament branch physical object quality defining the morphology (shape, size, length) of branches in relationship to the main filament.
straight filament branch
The nature of branches in a microbial filament to be straight (i.e. not curved).
arcuate filament branch
branches later curve to the direction of the original trichome
The nature of branches in a microbial filament to be arcuate (curved).
solitary branches
Filament branch spatial pattern where the filament exhibits branching (either false or true branching). Filaments occur singularly (solitary), and do not occur in pairs (are offset from one another).
paired branches
Filament branch spatial pattern where the filament exhibits branching (either false or true branching). Filaments occur paired, opposite from one another.
heterocyte position relative to branches
Differentiated filament branching relating the distribution of heterocytes in the filament and their spatial relationship to the branches.
branches thinner than main filament
secondary lateral branches generally thinner
Filament branch size where the diameter of cylindrical cells in the filament branches are thicker than the diameter of cylindrical cells in the main trichome.
heterocytes basal in branches
The physical relationship of branches in a microbial filament to heterocytes, being physically adjacent to heterocytes wherein branches initiate at the heterocytes.
lateral branches initiating at the heterocytes
branches initiate usually at originally intercalary heterocytes
heterocytes apical in branches
The physical relationship of branches in a microbial filament to heterocytes, where heterocytes are located at the end of branches.
heterocytes not in branches
Heterocyte position where the heterocyte is not located in the filament branch.
Heterocyte position where the heterocyte is located in the filament branch. Heterocyte location within the filament branch has apical-basal polarity.
paired false branching
Paired filament branches where the filament exhibits false branching.
paired true branching
Paired filament branches where the filament exhibits true branching.
solitary false branching
Solitary filament branches where the filament exhibits false branching.
solitary true branching
Solitary filament branches where the filament exhibits true branching.
Filament differentiation quality, relating to the morphology and differentiation of the apical cell in the filament.
A trichome cell that is located subterminally in a trichome, and which has undergone differentation. Found in trichomes with apical-basal polarity.
short filament branch
Filament branch length where the branches are short and contain few cells (relative to the length and number of cells in the main filament).
long filament branch
Filament branch length where the branches are long and contain many cells (relative to the length and number of cells in the main filament).
The molar percentage of nucleobases in the DNA of a prokaryotic chromosome and/or plasmid.
A prokaryotic cell that is bacillus-like (some variation of cylindrical).
Flagellated cell where the cell has multiple flagella, located in two tufts at both ends (poles) of the cell.
prokaryotic acidic environment
An acidic environment that is the environment inhabited by a prokaryotic cell.
prokaryotic alkaline environment
An alkaline environment that is the environment inhabited by a prokaryotic cell.
An assay to determine macroscopic or microscopic optical quality of a prokaryotic cell, or aggregation of prokaryotic cells.
Prokaryotic metabolite where fermentation produces are produced as a result of fermentation.
A type of filament only found in some filamentous cyanobacterial taxa. Is comprised by a trichome and an extracellular sheath. May or may not have branches.
An isopolar trichome morphology quality where heterocysts are only present at the poles, or terminals, of the trichome such that the trichome morphology is symmetric.
An isopolar trichome morphology quality where heterocysts are regularly dispersed along the length of the trichome such that the trichome morphology is symmetric.
Trichome differentiation quality of isopolar trichomes with respect to the location of heterocytes and the symmetrical arrangement of the heterocytes.
A part of a trichome where rapid cell division occurs. Meristematic zones, which arise terminally or subterminally, are found in the trichomes of Scytonemataceae, Rivulariaceae, and Microchaetaceae. Zone involves several cells along the trichome.
A sheath that is easily dissolving and indistinct (unlocalized).
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/CaseinAgar.html FORMULA Ingredients per liter of deionized water:* Dry Milk, Instant Nonfat 50.0gm Pancreatic Digest of Casein 5.0gm Yeast Extract 2.5gm Glucose 1.0gm Agar 12.5gm Final pH 6.8 +/- 0.3 at 25 degrees C.
An organic-rich, solid microbiological culture medium that contains nonfat dry milk, pancreatic digest of casein (casitone), yeast extract, glucose, and agar. Used for the differentiation of prokaryotes based on their ability to degrade casein.
A group of prokaryotic cells where the cells are attached to one another end-to-end, forming long threads. Formed when cell division occurs in a single plane (at right angles to the filament axis) and when cells do not detach from one another.
A trichome part formed when there is transient cell division in another plane other than crosswise to the length of the trichome axis. This results in the formation of a branch at right angles to the axis of the length of the trichome. Found only in the Stigonematales (Cyanobacteria).
A trichome part formed when there is interruption of the axis of a trichome. Are initiated when necritic cells form, breaking the trichome. Or are initiated at heterocytes. At first both ends of the interrupted trichome grow aside one another, later on the two new trichome ends grow in different directions. Occurs in the Oscillatoriales, Nostocales, and Stigonematales (Cyanobacteria).
A filament part formed when a new trichome begins to grow at an angle to the axis of the original length of the filament, also enclosed in sheath.
A sheath that is firm and distinct (localized, not easily dissolving and not indistinct).
sheath having distinctive spatial pattern
Morphologically differentiated filament part, where the sheath has a distinctive spatial pattern.
A prokaryotic cell containing thylakoids membranes.
epilithic environment
An environment that exists on the surface of a rock (or a rocky substrate).
epipelic environment
A submerged environment consisting of a rocky substrate comprised of sediments, clays, or silt, colonized by a microorganisms or a microbial community.
epiphytic environment
Wikipedia: Epiphyte An epiphyte is a plant that grows harmlessly upon another plant (such as a tree) and derives its moisture and nutrients from the air, rain, and sometimes from debris accumulating around it. Epiphytes differ from parasites in that epiphytes grow on other plants for physical support and do not necessarily negatively affect the host. An epiphytic organism that is not a plant is called an epibiont.[1] Epiphytes are usually found in the temperate zone (e.g., many mosses, liverworts, lichens, and algae) or in the tropics (e.g., many ferns, cacti, orchids, and bromeliads).[2] Many houseplants are epiphyte species due to their minimal water and soil requirements. Epiphytes provide a rich and diverse habitat for other organisms including animals, fungi, bacteria, and myxomycetes.[3]
An environment that exists on a terrestrial plant (Embryophyte).
endophytic environment
An environment that exists within a plant (Embryophyte).
Wikipedia: Endophyte An endophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its life cycle without causing apparent disease.[1][2][3][4] Endophytes are ubiquitous and have been found in all species of plants studied to date; however, most of these endophyte/plant relationships are not well understood.[5][6] Endophytes are also known to occur within lichens[7] and algae. Many economically important grasses (e.g., Festuca spp. and Lolium spp. ) carry fungal endophytes in genus Epichloë,[8] some of which may enhance host growth[9] and may improve the plant's ability to tolerate abiotic stresses, such as drought, and enhance resistance to insects, plant pathogens and mammalian herbivores.[10][11][12][13]
periphytic environment
An environmental system that is submerged, and attached to submerged rocks, plants, animals, or other objects.
Modified apical cell, having a thickened or enlarged morphology. Has a hood-like, lid-like, or cap-like form. Little is known about the role, although presence is environmentally influenced. Not all trichomes in a natural population will exhibit a calyptra. Found in the Oscillatoriales and Nostocales.
http://huey.colorado.edu/cyanobacteria/taxa/calyptra.php Definition of Calyptra Thickened or enlarged tip of a cyanobacterial filament, a hoodlike, lidlike, or caplike structure.
A prokaryotic colony that has a distinct morphology (size, shape, texture, or structure).
trichomes narrowed in the middle part and clearly widened at the ends
Tapered trichome quality where the trichome is wider at the apical and basal ends, and narrower toward the center of the trichome (hour-glass shaped).
Prokaryotic quality (morphology, symmetry, position) relating to a cell part or cellular component.
trichomes attenuated at the ends of developed trichomes
Tapered trichome quality where the trichome is narrower at the apical and basal ends, and widened toward the center of the trichome (fusiform-shaped).
Thylakoid-containing cell where there are widened spaces between the thylakoid membranes. Function is not clear and are likely influenced by environmental factors. Frequency and location are taxa-specific.
Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (detergent).
A gravity-driven water filter made by the British Berkefeld® company, with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter.
An organic rich, liquid marine microbiological culture medium containing acetate, tryptone, yeast extract, citrate, ammonium nitrate, with a seawater base. Used for the cultivation of Marispirillum.
From: Lai, Q., Yuan, J., Gu, L. & Shao, Z. (2009). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281. Recipe: sodium acetate, 1.0 g Tryptone, 10.0 g yeast extract, 2.0 g sodium citrate, 0.5 g NH4NO3, 0.2 g seawater, 1L pH 7.5
From: Lai, Q., Yuan, J., Gu, L. & Shao, Z. (2009). Marispirillum indicum gen. nov., sp. nov., isolated from a deep-sea environment. Int J Syst Evol Microbiol 59, 1278–1281. Recipe: sodium acetate, 1.0 g Tryptone, 10.0 g yeast extract, 2.0 g sodium citrate, 0.5 g NH4NO3, 0.2 g seawater, 1L pH 7.5 Agar is added to the medium to solidify.
An organic rich, solid marine microbiological culture medium containing acetate, tryptone, yeast extract, citrate, ammonium nitrate, with a seawater base. Used for the cultivation of Marispirillum.
An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Used for the cultivation of heterotrophic marine microorganisms.
From: http://himedialabs.com/TD/M384.pdf Zobell Marine Agar 2216 Zobell Marine Agar 2216 is recommended for cultivation, isolation and enumeration of heterotrophic marine bacteria. Composition Ingredients (Gms/Litre) Peptic digest of animal tissue 5.000 Yeast extract 1.000 Ferric citrate 0.1000 Sodium chloride 19.450 Magnesium chloride 8.800 Sodium sulfate 3.240 Calcium chloride 1.800 Potassium chloride 0.550 Sodium bicarbonate 0.160 Potassium bromide 0.080 Strontium chloride 0.034 Boric acid 0.022 Sodium silicate 0.004 Sodium fluorate 0.0024 Ammonium nitrate 0.0016 Disodium phosphate 0.008 Final pH (25˚C) 7.6+/-0.2
A culture medium used to select for, grow, and maintain prokaryotic microorganisms. Can be in either liquid (broth) or solidified (e.g. with agar) forms.
A chemically defined microbiological culture medium where all chemicals used are known. Does not contain any plant, animal, or microbiological cell extracts.
A microbiological culture medium that is liquid.
A microbiological culture medium that is solidified with a gelling agent, such as agar, agarose, gelatin, or pectin.
undefined microbiological culture medium
A microbiological culture medium that has components that are chemically heterogeneous, unknown, or uncharacterized mixtures or cellular extracts.
pH is not noted in the original publication. However, pH of the medium is reported to be 7.0 in The Prokaryotes: Vol 3: Archaea. Bacteria: Firmicutes, Actinomycetes, p. 213, copyrighted 2006.
A mineral-salts liquid microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with H2 and CO2 in the headspace gas mixture. Used for the growth of methanogenic archaea.
A mineral-salts, liquid microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with hydrogen and carbon dioxide in the headspace gas mixture. Used for the growth of methanogenic archaea.
From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. Ingredients are added to distilled water to give a final volume of 1L. Cysteine and Na2S are added after boiling the medium under an 80% N2-20% CO2 gas mixture, the final gas phase of tubed medium being an 80% H2-CO2 gas mixture at two atmospheres of pressure. 50 mL mineral 1 solution (6 g K2HPO4 per liter) 50 mL mineral 2 solution (per liter: KH2PO4, 6 g; (NH4)2SO4, 6 g, NaCl, 12 g; MgSO4x7H2O, 2.6 g; CaCl2x2H2O, 0.16 g) 10 mL trace minerals (pH to 7.0 with KOH; per liter: nitrilotriacetic acid, 1.5 g; MgSO4x7H2O, 3.0 g; MnSO2x2H2O, 0.5 g; NaCl, 1.0 g; FeSO4x7H2O, 0.1g; CoSO4 or CoCl2, 0.1 g; CaCl2x2H2O, 0.1 g; ZnSO4, 0.1 g; CuSO4x5H2O, 0.01 g; AlK(SO4)2, 0.01 g; H3BO3, 0.01 g; Na2MoO4x2H2O, 0.01g); dissolve nitrilotriacetic acid with KOH to pH 6.5 then proceed to add minerals) 10 mL trace vitamines (contains in milligrams per liter: biotin, 2; folic acid, 2; pyridoxine hydrochloride, 10; thiamine hydrochloride, 5; riboflavin, 5; nicotinic acid, 5; DL-calcium pantothenate, 5; vitamin B12, 0.1; p-aminobenzoic acid, 5; lipoic acid, 5) 0.002 g FeSO4x7H2O 5.0 g NaHCO3 2.5g sodium acetate 2.5 g sodium formate 2.0 g yeast extract 2.0 g trypticase (BBL) 0.5 g L-Cysteine hydrochloridexH2O 0.5 g Na2Sx9H2O
A dilute, mineral-salts liquid microbiological culture medium formulated to mimic Lake Kinneret (Sea of Galilee) water. Used to cultivate freshwater autotrophic microorganisms.
Could not find in any references the pH of AKW, however the pH of Lake Kinneret varies from about 8.6 at the surface to 7.8 at depth. http://kinneret.ocean.org.il/ar_grp.aspx
From Table 1 in: Sherr BF, Sherr EB, Berman T. 1983. Grazing, growth, and ammonium excretion rates of a heterotrophic microflagellate fed with four species of bacteria. Appl Environ Microbiol 45(4):1196-1201. Salt Amt (mg L-1 of distilled water) NaHCO3 197 NaCl 155 CaCl2 130 MgCl2 124 MgSO4 65 KCl 13 CaSO4 9.5 Total salts, 693.5 mg L-1. Total solids in Lake Kinneret water range from 640 to 714 mg L-1.
Prokaryotic cell part quality, describing the nature of the cell wall. Determined from the results of the Gram stain assay.
A hypersaline, liquid microbiological culture medium that contains mineral salts, peptone, and yeast extract. Used for the cultivation of Haloarchaeobium iranensis.
From:Makhdoumi-Kakhki et al 2011. Haloarchaeobium iranensis gen.nov., sp. nov., an extremeley halophilic archaeon isolated from the saline lake Aran-Bidgol, Iran. Int J. Syst. Evol. MIcrobiol. doi:10.1099/ijs.0.033167-0 and Dyall-Smith. 2009. Halohandbook. http://www.haloarchaea.com/resources/halohandbook/ Add the following to a large beaker: Salt water (30% stock), 767 Pure water, 200 Peptone (Oxoid), 5 yeast extract, 1 Stir to dissolve, adjust pH up to 7.5 with 1M Tris pH 7.5, using 5 mL per liter. Adjust to 1L with pure water. For solid medium add 15g Difco Bacto-agar. 30% Salt water stock solution (the CaCl2 is not added until just before pouring plates and some TrisHCl pH 7.5 is added): per liter: H2O, 850 mL NaCl, 240 g MgCl2x6H2O, 30g KCl, 7g 1M TrisHCL pH 7.5, 20 mL
From: Zhou H et al. 2008. Isolation and characterization of Ferroplasma thermophilum sp. nov., a novel extremely acidophilic, moderately thermophilic archaeon and its role in bioleaching of chalcopyrite. J Appl Microbiol 105:591. Modified 9K medium contained (per litre): 3.0 g (NH4)2SO4, 0.5 g KH2PO4, 0.1 g KCl, 0.5 g MgSO4x7H2O, 0.01 g Ca(NO3)2. Trace elements (per litre) comprised: 11.0 mg FeCl3x6H2O, 0.5 mg CuSO4x5H2O, 50 mg Na2SO4, 2.0 mg H3BO3, 2.0 mg MnSO4, 0.8 mg Na2MoO4x2H2O, 0.6 mg CoCl2x6H2O, 0.9 mg ZnSO4x7H2O and 0.1 mg Na2SeO4. The pH of the medium was adjusted to 1.0 by adding 50% (v ⁄ v) H2SO4, was autoclaved, and then filter-sterilized (0.2 um filter paper) trace elements and FeSO4x7H2O were added in the medium.
An acidic, liquid, mineral-salts microbiological culture medium, high in ferrous iron. Used for the cultivation of Ferroplasma thermophilum.
From: Heng-Lin Cui, Xin-Yi Li, Xia Gao, Xue-Wei Xu, Yu-Guang Zhou, Hong-Can Liu, Aharon Oren and Pei-Jin Zhou. 2010. Halopelagius inordinatus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. IJSEM 60:2089-2093. per liter: yeast extract, 0.2 g fish peptone, 0.2 g sodium pyruvate, 2.0 g sodium lactate, 2.0 g KCl, 5.4 g K2HPO4, 0.3 g NH4Cl, 0.5 g MgSO4x7H2O, 20.0 g NaCl, 200.0 g pH 7.0-7.2.
neutral oligotrophic haloarchaeal medium
A hypersaline, liquid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, lactate, peptone, and yeast extract. Used for the cultivation of Halopelagius inordinatus.
A hypersaline, liquid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, glucose, glutamate, citrate, and peptones. Used for the cultivation of Halogranum rubrum and Halosarcina limi.
From: Cui H-L, et al. 2010. Halogranum rubrum gen. nov., sp. nov., a halophilic archaeon isolated from a marine solar saltern. Int J Syst Evol Microbiol 60:1366. Modified R2A medium: Contains the following ingredients per liter: Casamino acids 0.5g yeast extract, 0.5g sodium pyruvate, 0.5 g fish peptone, 0.5g glucose, 0.5g sodium glutamate, 0.5g trisodium citrate, 3.0g KCl, 2.0g K2HPO4, 0.3g CaCl2, 0.5g MgSO4x7H2O, 20.0g NaCl, 200.0 g pH 7.0-7.2
From: Cui H-L et al. 2010. Halosarcina limi sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halosarcina. Int J. Syst. Evol. Microbiol. 60:2462. CM2 Recipe: Per liter: Casamino acids (Difco), 0.5 g yeast extract (Difco), 0.5 g sodium pyruvate, 0.5 g fish peptone, 0.5 g glucose, 5.0 g sodium glutamate, 0.5 g trisodium citrate, 3.0 g KCl, 2.0 g K2HPO4, 0.3 g CaCl2, 0.5 g MgSO4x7H2O, 20 g NaCl, 230.0 g pH 7.0–7.2
Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is known, it is referred to as "defined".
DSM strains: 19505 Haladaptatus cibarius 18796 Halalkalicoccus jeotgali B3 14522 Halococcus dombrowskii 19504 Halorubrum cibi 18794 Haloterrigena jeotgali A29 18795 Natronococcus jeotgali DSM 18795
From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium954.pdf 954. Medium for Halococcus dombrowskii. Casamino acids 5.00 g Yeast extract 5.00 g TRIS 12.10 g KCl 2.00 g MgCl2x6H2O 20.00 g CaCl2x2H2O 0.20 g NaCl 200.00 g Agar 20.00 g Distilled water 1000.00 mL Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH. © 2007 DSMZ GmbH - All rights reserved
A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, tris, casamino acids, and yeast extract. Used to cultivate Halococcus dombrowskii (DSM 14522).
From:http://www.atcc.org/~/media/B61DC4844DBE4D51867A06D49DE22564.ashx ATCC Medium: 2820 HAYFLICK Medium Yeast Extract…………………………………..19.6 g PPLO…………………………………………...17.7 g Phenol Red……………………………………24 mg DNA sodium salt (Sigma, D1501)………...…0.24 g Horse serum (aseptically add)……………….157 ml DI Water………………………………………..843 ml Combine all ingredients except for the horse serum. Adjust pH to 7.8 +/- 0.2. Autoclave at 121C and let medium cool to 55C. Aseptically add horse serum and dispense as required.
An organic-rich, liquid microbiological culture medium containing beef heart infusion, peptone, and sodium chloride. Developed for the growth of Mycoplasma pneumoniae.
From: Nutrient Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Nutrient Broth has the formula originally designed for use in the Standard Methods for the Examination of Water and Wastewater. It is not a recommended bacteriological medium in later editions of this publication. It is one of several nonselective media recommended for use in the Most Probable Number (MPN) technique of estimating the density of viable organisms in food samples1 and is useful in routine cultivation of microorganisms. Principles of the Procedure This relatively simple formulation supports the growth of nonfastidious microorganisms due to its content of peptone and beef extract. Formula Difco™ Nutrient Broth Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Peptone....................................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2
An organic-rich, liquid culture medium comprised of beef extract and peptone.
Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997.: When it was desirable to avoid attack of the substratum, strains were grown on ZoBell solidified with 0.7% (w/v) Phytagel (a gellan gum; Sigma).
An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Solidified using Phytagel (gellan gum) instead of agar. Used for the cultivation of heterotrophic marine microorganisms capable of degrading agar.
A microbiological culture medium that contains bovine rumen fluid (or ruminal fluid), used for the culture of microorganisms that live in the rumen.
Sehgal and Gibbons, 1960, Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6:165.
A hypersaline, organic-rich liquid medium containing casamino acids, yeast extract, and salts. Used for the growth of Halobacterium cutirubrum.
Original citation: Sehgal SN, Gibbons NE (1960) Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6:165–169. The routine medium contained 7.5 g casamino acids (Difco), 10 g yeast extract (Difco), 3 g trisodium citrate, 2 g potassium chloride, 20 g magnesium sulphate heptahydrate, 250 g sodium chloride, aid distilled water to 1 liter. The ingredients were dissolved in 800 ml water, the pH adjusted to 7.5-7.8 with N potassium hydroxide, and the medium autoclaved 5 minutes at 120˚C. It was then filterecl to remove the precipitate, the pH mas adjusted to 7.4 with N hydrochloric acid, and the medium made up to final volume.
From: Schneegurt MA, Chapter 2, Media and conditions for the growth of halophilic and halotolerant bacteria and archaea. pg. 39, Table 2.1. In Vreeland RH (ed) Advances in Understanding the Biology of Halophilic Microorganisms. DOI 10.1007/978-94-007-5539-0_2 Medium composition in grams per liter: NaCl, 250 MgSO4x7H2O, 20 FeCl2, 0.023 Na-citrate, 3 Casamino acids, 7.5 yeast extract, 10
From: Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 is used for the isolation and cultivation of fastidious, obligately anaerobic microorganisms.
An organic-rich, solid microbiological culture medium, containing peptones, yeast extract, glucose, and sodium bisulfite as a reducing agent. Supplemented with 5% sheep blood, hemin, and vitamin K. Used for the growth of Brucella.
An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Solidified using Phytagel instead of agar. Supplemented with starch. Used for the cultivation of heterotrophic marine microorganisms capable of degrading agar.
Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997. The strain was assayed for amylase activity using starch at a concentration of 0.2 % (w/v) in ZoBell agar or in ZoBell Phytagel plates.
Balch et al. 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260.
A liquid, mineral-salts microbiological culture medium containing acetate, formate, yeast extract, trypticase, cysteine and sulfide with H2 and CO2 in the headspace gas mixture. Supplemented with additional 0.1% NH4Cl, 10% rumen fluid, 2% agar, and with clindamycin and cephalotin (cefalotin). Used for the growth of Methanobrevibacter from feces.
Miller, 2001. In Bergey's Manual of Systematic Bacteriology, v. 1, 2nd ed. p.219.
Miller TL et al, 1982. Isolation of Methanobrevibacter smithii from human feces. Appl Environ Microbiol 43(1):227.
A hypersaline, organic-rich, solid microbiological culture medium that contains mineral-salts, tris, peptone, and yeast extract. Used for the cultivation of Haloarchaeobium iranensis.
microbiological culture medium containing blood
A microbiological culture medium that contains blood, treated blood, lysed blood cells, blood serum, or treated blood serum.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Animal Blood, Citrated is blood that has been washed and treated with sodium citrate as the anticoagulant. It serves as a source of erythrocytes for serological procedures.
Blood medium ingredient comprised of animal blood where citrate has been added to prevent coagulation. Used in the cultivation of microorganisms.
equine serum-containing microbiological culture medium
A liquid microbiological culture medium that contains horse (equine) serum.
microbiological culture medium, containing procine serum
A microbiological culture medium (a blood serum agar), made with porcine (pig) blood serum.
An organic-rich, liquid microbiological culture medium containing various peptones and sodium chloride. Developed for the growth of mycoplasmas.
From: PPLO Media (Mycoplasma Media) (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use PPLO (Mycoplasma) agars and broths, when supplemented with nutritive enrichments, are used for isolating and cultivating Mycoplasma. Mycoplasma Broth Base (Frey) is used for the cultivation of avian mycoplasmas. Principles of the Procedure Meat digests, peptones, beef extract and yeast extract provide the nitrogen, vitamins, amino acids and carbon in these media. Sodium chloride maintains the osmotic balance of these formulations. Agar, the solidifying agent, is used in PPLO (Mycoplasma) Agar at a concentration slightly reduced from usual to ensure formation of the largest possible colonies because the organisms grow into the agar with only slight surface growth.13 The base media are supplemented with Mycoplasma Supplement or Mycoplasma Enrichment w/o Penicillin because Mycoplasma spp. are fastidious in their growth requirements. 14 Mycoplasma Supplement contains fresh yeast extract and horse serum. Yeast extract provides the preformed nucleic acid precursors that are required by Mycoplasma spp.14 Horse serum supplies cholesterol, a growth stimulant.14 Mycoplasma Enrichment without Penicillin is a selective enrichment containing the inhibitor thallium acetate, to which a penicillin of choice (penicillin G or a broad-spectrum semisynthetic penicillin) can be added at the time of use to make it selective against gram-positive and gram-negative bacteria. Formulae Difco™ PPLO Agar Approximate Formula* Per Liter Beef Heart, Infusion from 50 g..................................... 6.0 g Peptone..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 14.0 g Difco™ PPLO Broth Consists of the the same ingredients without the agar. pH 7.8 ± 0.2 BBL™ Mycoplasma Agar Base (PPLO Agar Base) Approximate Formula* Per Liter Beef Heart, Infusion from (solids).................................. 2.0 g Pancreatic Digest of Casein.......................................... 7.0 g Beef Extract.................................................................. 3.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 14.0 g pH 7.8 ± 0.2 BBL™ Mycoplasma Broth Base (PPLO Broth Base) Consists of the same ingredients without the agar. BBL™ Mycoplasma Broth Base (Frey) Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 7.5 g Papaic Digest of Soybean Meal..................................... 2.5 g Yeast Extract................................................................ 5.0 g Sodium Chloride.......................................................... 5.0 g Potassium Chloride...................................................... 0.4 g Magnesium Sulfate...................................................... 0.2 g Disodium Phosphate.................................................... 1.6 g Monopotassium Phosphate.......................................... 0.1 g pH 7.7 ± 0.2 Difco™ Mycoplasma Supplement Approximate Formula* Per 30 mL Vial Yeast Extract................................................................ 0.09 g Horse Serum.............................................................. 22.8 mL BBL™ Mycoplasma Enrichment without Penicillin Approximate Formula* Per 30 mL Vial Horse Serum.............................................................. 20.0 mL Yeast Extract (fresh autolysate)................................... 10.0 mL Thallium Acetate........................................................ 50.0 mg *Adjusted and/or supplemented as required to meet performance criteria.
An organic-rich, liquid microbiological culture medium containing pancreatic digests of casein and gelatin, PPLO broth (without CV, i.e. without crystal violet), fetal bovine serum (fetal calf serum), CMRL 1066 medium (a mixture of amino acids, vitamins, co-factors, and organic micronutrients), yeast extract, and polymyxin B, Amphotericin B, and penicillin. Used for the growth of mycoplasmas.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/SP4Media.htm SP4 Broth, Plain**: Pancreatic Digest of Casein 10.0gm Pancreatic Digest of Gelatin 5.0gm PPLO Broth without CV 3.5gm Polymyxin B 50.0mg Amphotericin B 5.0mg Fetal Bovine Serum 170.0ml CMRL 1066 Medium (10X) 50.0ml Yeast Extract 35.0ml Yeastolate 10% 20.0ml Penicillin 1,000,000U Final pH at 25 degrees C.: SP4 Broth, Plain SP4 Broth with Arginine 7.0 +/- 0.2
A solid microbiological culture medium (a blood agar), containing horse blood.
A slant culture is where liquified (melted) solid medium has been added to a sterile test tube and allowed to solidify (cool) at an angle with respect to the horizontal, such that the solid medium surface is at an angle with respect to the sides of the test tube. The slant culture allows for a larger surface of the agar to be exposed to oxygen.
A mineral-salts, marine, liquid microbiological culture medium containing soil extract. Used for the growth of photoautotrophic microorganisms.
http://www-cyanosite.bio.purdue.edu/media/table/bg.html From: Moss,B. 1972. The influence of environmental factors on the distribution of freshwater algae: an experimental study. J. Ecol. 60:917-932. Blue-Green (BG) Medium This medium is made up in 2 parts. Autoclave parts 1 and 2 separately at 15 psi, allow to cool them then mix aseptically. For agar plates, add 15 g non-nutrient agar per L. Part 1: Tricine 0.50 g Soil extract (SE1) 25.00 mL Extra nutrient salts 3.75 mL Filtered natural seawater to 1.0 L Adjust pH to 7.6-7.8 with 1M NaOH or HCl. Part 2: NaNO3 1.500 g K2HPO4x3H2O 0.040 g MgSO4x7H2O 0.075 g CaCl2x2H2O 0.036 g Citric acid 0.006 g Ammonium ferric citrate green 0.006 g EDTANa2 0.001 g Na2CO3 0.020 g Trace metal solution 1.00 mL Distilled water to 1.0 L Adjust pH to 7.4. Extra nutrient salts: NaNO3 3.00 g Na2HPO4 0.12 g K2HPO4 0.10 g Distilled water to 100.0 mL Trace metal solution: H3BO3 0.286 g MnCl2x4H2O 0.022 g Na2MoO4x2H2O 0.039 g CuSO4x5H2O 0.008 g Co(NO3)2x6H2O 0.005 g Distilled water to 100 mL
A mineral-salts, liquid microbiological culture medium comprised mainly of sodium bicarbonate. Used to cultivate autotrophic microorganisms from lakes.
From: Lindstrom, K. 1983. Selenium as a growth factor for plankton algae in laboratory experiments and in some Swedish lakes. Hydrobiologia 101:35-48. Macronutrients (all values mg per L) NaHCO3 84.00 NaNO3 12.75 CaCl2x2H2O 14.70 MgSO4x7H2O 12.32 K2HPO4 1.74 Na2SiO3x9H2O 1.14 Micronutrients FeCl3x6H2O 0.108 Na2MoO4x2H2O 0.048 ZnCl2 0.027 MnCl2x4H2O 0.010 CoCl2x6H2O 0.0096 Na2SeO3x5H2O 0.000184 EDTA 0.558 pH 8.1
A mineral-salts, liquid microbiological culture medium containing sodium acetate, potassium phosphate, sodium carbonate, ammonium sulfate, cysteine hydrochloride, sodium sulfide, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, nitrilotriacetic acid, manganese sulfate, cobalt chloride, zinc sulfate, resazurin, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, p-aminobenzoic acid (4-aminobenzoic acid), lipoic acid, biotin, folic acid, and vitamin B12 (cobalamin). Prepared under an atmosphere of dinitrogen and carbon dioxide. Used for the growth of Methanosaeta concillii.
From: Patel GB. 1984. Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic, aceticlastic methanogen. Can J Microbiol 30:1383-1396. The composition of the Aa medium used for isolation and stock culture maintenance was as follows (in milligrams per litre): CH3COONa·3H2O, 6800; K2HPO4, 2190; KH2PO4, 1500; Na2CO3, 480; (NH4)2SO4, 450; cysteine-HCl, 125; Na2S·9H2O, 125; MgSO4·7H2O, 120; CaCl2·2H2O, 60; NaCl, 54; FeSO4 ·7H2O, 21; nitrilotriacetic acid, 15; MnSO4 · 2H2O, 5; CoCl2-6H2O, 1; ZnSO4·7H2O, I; resazurin, I; CuSO4·5H2O, 0.1; AIK(SO4)2- 12H2O, 0.1; H3BO3, 0.1; Na2Mo04·2H2O, 0.1; pyridoxine-HCl, 0.1; thiamine-HCl, riboflavin, nicotinic acid, p-aminobenzoic acid, lipoic acid, each 0.05; biotin and folic acid, each 0.02; vitamin B-12, 0.005. The Aa medium ingredients, except Na2CO3 and cysteine- Na2S, were 'mixed and adjusted to pH 7.8. The medium was then reduced with cysteine- Na2S, and Na2CO3 was added, using the Hungate (1950) technique. The reduced medium was dispensed in 10-mL aliquots into 60-mL serum vials (Miller and Wolin 1974) under 80% N2: 20% CO2 gas phase. The postautoclave (121°C, 15 min) pH was 7.2 ± 0.1. If the same medium contained 10000 mg/L of CH3COONa·3H20, it was referred to as AA medium.
Quality that inheres in a prokaryotic cell, group of cells, prokaryotic colony, or part of a prokaryotic cell.
L-proline arylamidase assay using nitroanilide
L-proline arylamidase that uses the substrate L-proline 4-nitroanilide (L-proline-p-nitroanilide). Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) cleaves the substrate, releasing 4-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
A filter with a specified pore size that filters solid material above the specified pore size out of a liquid medium.
A gravity-driven water filter made by the British Berkefeld® company.
Physical object quality of a prokaryotic cell.
A trichome part, refering to the poles (terminal ends) of a prokaryotic trichome. May involve one cell or multiple cells along the trichome.
Temperature optimum quality, where growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures.
An organic-rich, mineral-salts, solid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Used for the cultivation of heterotrophic marine microorganisms.
From: Bacto™ Tryptic Soy Broth/Trypticase™ Soy Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Enzymatic digests of casein and soybean provide amino acids and other complex nitrogenous substances. Dextrose is an energy source. Sodium chloride maintains the osmotic equilibrium. Dibasic potassium phosphate acts as a buffer to control pH. The addition of 6.5% sodium chloride to Trypticase Soy Broth permits the differentiation of salt-tolerant enterococci, which are resistant to the high salt content, from the salt-intolerant S. bovis group and other streptococcal species. At this concentration, the sodium chloride is a selective agent that interferes with membrane permeability and osmotic and electrokinetic equilibria.4 Fildes Enrichment is a peptic digest of sheep blood that supplies the X (hemin) and V (nicotinamide adenine dinucleotide, NAD) factors necessary for the growth of H. influenzae. Dextrose is omitted from the formula for Tryptic Soy Broth without Dextrose to permit use of the medium in fermentation studies. The carbohydrate concentration used most frequently in fermentation reactions is 0.5% or 1%. Tryptic Soy Broth and Trypticase Soy Broth are provided as prepared media in a variety of bottle styles. In addition, Tryptic Soy Broth is provided as a Sterile Pack Bottle; i.e., the bottle has been terminally sterilized inside of autoclavable double-bags. All varieties of bottled TSB conform with requirements for Ready- To-Use Media as described in the USP. Formulae Bacto™ Tryptic Soy Broth (Soybean-Casein Digest Medium) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Dextrose...................................................................... 2.5 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g pH 7.3 ± 0.2 BBL™ Trypticase™ Soy Broth (Soybean-Casein Digest Broth) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Papaic Digest of Soybean............................................. 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g Dextrose...................................................................... 2.5 g pH 7.3 ± 0.2 Bacto™ Tryptic Soy Broth without Dextrose Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 17.0 g Enzymatic Digest of Soybean Meal............................... 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 2.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2
An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), dextrose (D-glucose), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate).
Wikipedia:Tryptic_soy_broth Tryptic soy broth (frequently abbreviated as TSB) is used in microbiology laboratories as a culture broth to grow aerobic bacteria. It is frequently used in commercial diagnostics in conjunction with the additive Thio which promotes growth of anaerobes.
An organic-rich, solid medium containing casitone (pancreatic digest of casein), Phytone peptone (papaic digest of soybean), and sodium chloride. Used for the cultivation of aerobic heterotrophic microorganisms.
From: Tryptic Soy Agar/Trypticase™ Soy Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptic (Trypticase) Soy Agar (TSA) is used for the isolation and cultivation of nonfastidious and fastidious microorganisms. It is not the medium of choice for anaerobes. Principles of the Procedure The combination of casein and soy peptones in TSA renders the medium highly nutritious by supplying organic nitrogen, particularly amino acids and longer-chained peptides. The sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. Haemophilus species may be differentiated by their requirements for X and V factors. Paper strips impregnated with these factors are placed on the surface of the medium after inoculation with the test organism. Following incubation, a zone of growth around the strip indicates a requirement for the factor(s). Formulae Difco™ Tryptic Soy Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean............................................. 5.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 BBL™ Trypticase™ Soy Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean............................................. 5.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria.
A single-organism metabolic process that is present in a prokaryotic metabolically differentiated cell.
chemical mixture, serving as microbiological medium ingredients
A solid or liquid mixture of chemicals which are used as ingredients in microbiological culture media used to grow and propogate prokaryotic microorganisms.
sub-cylindrical
A flattened prokaryotic cell where the shape is partially spherical.
Temperature optimum quality, where growth rates at elevated temperatures (40-85˚C) are higher than growth rates at ambient temperatures.
filament septation
A filament of prokaryotic cells relating to the presence or absence of septa separating the individual cells that make up the filament.
cyanobacterial filament part
distinctively shaped filament
A cell filament that has a distinctive shape.
spatially differentiated filaments
A filament morphological quality where the filaments have a particular spatial orientation with respect to a substrate or surface.
scytonematoid-branched filaments
False-branched filaments, where branches initiate at a necridic cell. Branches often appear as pairs.
filaments make sometimes typical loop-like lateral formations
tolypothrixoid-branched filaments
False-branched filaments, where branches initiate at a heterocyte. Branches only sometimes appear as pairs.
X-branched filaments
True-branched filaments, where the cells divide irregularly in different planes and form clusters of cells. The cells remain physiologically attached.
T-branched filaments
true branching lateral (usually unilateral)
erect true branches develop usually unilaterally after the lengthwise cell division
zzzz 'inheres in' some (Stigonema or Fischerella or Hapalosiphon or Westiella) Westiella is in NCBI Tax browser, but not in OntoFox.
True-branched filaments, where a cell in the trichome divides longitudinally (parallel to the axis of the filament) and the trichome branch starts to grow perpendicular to one side of the main trichome.
V-branched filaments
pseudodichotomous branches arise by longitudinal division of the apical cells
pseudodichotomously divaricated branches
zzzz 'inheres in' some (Hyphomorpha or Pulvinularia or Colteronema or Loriella or Mastigocoleopsis) - no NCBI entries.
pseudodichotomously divaricated at the ends
True-branched filaments, where an apical cell divides longitudinally, from which grows obliquely two equivalent branches.
pseudodichotomously divaricated free filaments
reverse Y-branched filaments
True-branched filaments, where branches arise in the intercalary (medial) cells after oblique cell division at an intercalary (medial cell).
zzzz 'inheres in' some (Scytonema or Brachytrichia or Mastigocladus) Brachytrichia is in NCBI Tax Browser, but not in OntoFox.
biseriate filament
Filament with one trichome in the sheath, where the trichome is two cells in width. Arises as a result of cell division occuring in two planes,one perpendicular to the axis of the trichome and one parallel to the axis of the trichome.
extracellular sheath
An extracellular sheath in cyanobacteria is typically a laminated (lamellated, laminar), fibrillar, extracellular structure made of polysaccharides (glycan). It plays a role in desiccation tolerance and often contains pigments (such as scytonemin) which protect from photodamage caused by uv light. It also has proteins (including water stress proteins, pectases). Often multilayered and colored.
trichome
Wikipedia: trichome Algal trichomes Certain, usually filamentous, algae have the terminal cell produced into an elongate hair-like structure called a trichome. The same term is applied to such structures in some cyanobacteria, such as Spirulina and Oscillatoria. Cyanobacteria trichomes may be unsheathed, as in Oscillatoria, or sheathed, as in Calothrix.[1] These structures play an important role in preventing soil erosion, particularly in cold desert climates.[citation needed] The filamentous sheaths form a persistent sticky network that helps maintain soil structure.
A prokaryotic filament part, comprised of a chain of cells that has undergone binary fission in a single plane, where the cells remain attached after cell division. Found in the Nostocales and Stigonematales (Cyanobacteria).
isopolar trichome
A prokaryotic trichome where the two poles of the trichome have the cell diameter (i.e., are symmetrical and not tapered). Caused by the development of a basal heterocyte.
morphologically not diversified in "main" filaments and branches
apical cells morphologically not different from other cells
heteropolar trichome
A prokaryotic trichome where the two poles of the trichome have different cell diameters (i.e. trichomes are tapered on end end and asymmetrical). Caused by the development of a basal heterocyte.
filaments differentiated into basal and apical parts
trichomes, oriented by their bases with heterocytes to their substrate and by their apical hir-like parts to the surface
filaments distinctly divided into basal end with a heterocyte and the apical end
differentiating into basal and apical parts
apical cell
A trichome cell that is located terminally in a trichome, and which has undergone differentiation. Found in trichomes with apical-basal polarity.
susceptibility to lysis by hypotonic solution
Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (hypotonic solution).
basal prostrate filaments
Filament orientation quality where erect filaments and prostrate filaments are present, refers to the basal filaments that are prostrate to the substrate.
necritic cell
trichomes disintegrating in separated cells within filaments
trichomes often disintegrate soon after heterocyte formation between heterocytes
A trichome cell comprised of a cell that has undergone differentiation such that it is dead (nectritic).
chroococcoid cell cluster
old trichomes change often in chroococcoid stages
Cell clusters, where the cells are chroococcoid (spherical).
differentiated meristematic zone
Filament differentiation quality with respect to the positioning of the meristematic zone along the length of the trichome.
tapered trichome
Heteropolar trichome where the width of the trichome varies such that the trichome is tapered.
hair cell
An apical cell or cells in a heteropolar filament that is differentiated to form an elongated hair-like extension.
in the apical part narrowed in elongated, cellular hair
trichomes elongated in cellular hair-like ends
vacuolized apical cell
Apical cell that is vacuolized (has gas vacuoles).
morphologically distinctive apical cell
Cyanobacterial filament part, where the apical cell is morphologically distinctive.
conical-rounded apical cell
Apical cell that is conical-rounded (conical-blunt).
conical apical cell
pointed apical cell
rounded apical cell
club-shaped ends of trichomes and branches
elongated apical cell
narrowed apical cell
Apical cell that is narrowed (has decreased width).
apical cell develops into nanocytes
terminal cells of branches divide often repeatedly in nanocytes
Apical cell quality related the developmental transformation of the apical cell into nanocytes.
hyaline apical cell
Apical cell that is hyaline (spindle-shaped).
elongated-rounded apical cell
Apical cell that is elongated-rounded (elongated-blunt).
bluntly pointed apical cell
Pointed apical cell that is bluntly pointed (attenuate).
sharply pointed apical cell
Pointed apical cell that is sharply pointed (acuminate).
vacuolized sub-apical cell
Sub-apical cell quality where the sub-apical cell is vacuolized (has gas vacuoles).
subterminal meristematic zones
Meristematic zone position quality where the position is subterminal.
terminal meristematic zones
in the apical part sometimes with meristematic zones
meristematic zons near the ends of branches
Meristematic zone position quality where the position is terminal.
tapered by apical widening
trichomes sometimes with slightly widened apical parts
Tapered trichome quality where the trichome is wider at the apical end, and narrower at the basal end (cuneate-shaped).
apical cell is larger than the other ones
tapered by apical narrowing
Tapered trichome quality where the trichome is narrower at the apical end, and wider at the basal end (attenuate-shaped).
From: Irgens RL. 1977. Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27(1):38-43. The TES, modified from Pfennig’s formula (personal communication), contained (per liter): ZnSO4x7H2O, 0.10 g; MnCl2x4H2O, 0.03 g; H3BO3, 0.3 g; CoCl2x6H20, 0.2 g; CuCl2x2H2O,0.01 g ; NiCl2x6H2O, 0.02 g; Na2MoO4x 2H2O, 0.03g; pH3 to 4.
A trace elements solution containing zinc sulfate, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate.
From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. Trace vitamines (contains in milligrams per liter: biotin, 2; folic acid, 2; pyridoxine hydrochloride, 10; thiamine hydrochloride, 5; riboflavin, 5; nicotinic acid, 5; DL-calcium pantothenate, 5; vitamin B12, 0.1; p-aminobenzoic acid, 5; lipoic acid, 5) Also reported in Wolin EA, Wolin MJ, Wolfe RS. 1963. Formation of methane by bacterial extracts. J Biol Chem 238(8):2882-2886.
A solution of vitamins containing biotin, folic acid, pyridoxine, thiamine, riboflavin, nicotinic acid, calcium pantothenate, cobalamin (vitamin B12), 4-aminobenzoic acid (p-aminobenzoic acid), and lipoic acid. Used to support growth of methanogenic archaea.
A chemical entity produced by a prokaryotic cell, as a result of metabolism.
Organic compounds or mixtures of organic compounds added to culture media to support growth or metabolism of a microorganism.
Prokaryotic metabolite where methane is produced as a result of methanogenesis.
Wikipedia:methanogenesis Methanogenesis or biomethanation is the formation of methane by microbes known as methanogens. Organisms capable of producing methane have been identified only from the domain Archaea, a group phylogenetically distinct from both eukaryotes and bacteria, although many live in close association with anaerobic bacteria. The production of methane is an important and widespread form of microbial metabolism. In most environments, it is the final step in the decomposition of biomass.
Prokaryotic metabolically differentiated cell, specialized for the production of methane. Found only in the archaeal domain of life.
A mixture of dry or liquid organic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is unknown or uncharacterized, it is referred to as "undefined".
The process in which tetrathionate is reduced to sulfide.
microbiological medium ingredient, derived from aqueous extracts of animal tissues or fluids
Undefined mixture of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of animal organs, animal tissues, or animal secretions. Used in the cultivation of microorganisms.
microbiological medium ingredient, derived from extracts of microbial organisms
Undefined mixtures of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of microbial (prokaryotic or microbial eukaryotic) cells. Used in the cultivation of microorganisms.
microbiological medium ingredient, derived from oil
Undefined mixtures of complex organic compounds derived from hydrophobic substances including lipids, fatty acids, and triacylglycerols, derived from the chemical or physical extraction of oil from an organism, the part of an organism, or a naturally occurring substance (such as crude oil).
microbiological medium ingredient, derived from extracts of proteinaceous material
An undefined mixture of complex organic compounds (peptides, sometimes with additional undefined vitamins and cofactors) derived from enzymatic, acid, or base digests of protein from plant or animal sources. Used in the cultivation of microorganisms.
microbiological medium ingredient, derived from chemical hydrolysis of protein
Undefined mixtures of complex organic compounds derived (typically amino acids) from the acid hydrolysis of animal or plant protein added to culture media.
microbiological medium ingredient, derived from enzymatic hydrolysis of protein
BD Bionutrients Technical Manual (3rd edition revised).
Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of protein.
Trademarked commercial name for pancreatic digest of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms.
Reference for Bio-trypticase: "Rapport de Mission au Centre National de l'Elevage et de Recherches Veterinaires de Nouakchott du 12/07 au 02/08/1999"; Institute Senegalais de Recherches Agricoles. Retired name for "peptone pancreatique du caseine"; produced by Bio-Merieux (www.biomerieux.com) or Oxoid (www.remel.com/Microbiology).
From BD Bionutrients Technical Manual (3rd edition revised): BBL™ Trypticase™ Peptone is a pancreatic digest of casein and is the primary nitrogen source in Trypticase Soy Broth and Agar. Ash content is 5.7% NaCl content is 0.1%
An enzymatic hydrolysis of meat (muscle tissue) proteins from mixed animal (mammalian) sources, used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised): Meat peptones are proteins from animal sources that have been hydrolyzed, or broken down into amino acids and peptides, to provide nitrogen for microorganisms. Meat peptones can be tailored to specific nutritive needs of microorganisms by controlling the quality and origin of the protein, the quality and source of the enzyme used to digest the protein, and the methods used for hydrolysis, concentration and drying the peptone. Peptone manufacture methods are discussed in the section titled Hydrolysis to Hydrolysate. Sources of animal protein include meat from muscle tissue or offal (waste parts, entrails) and gelatin. Muscular tissue and offal are utilized fresh, frozen or dried. Gelatin is extracted by boiling collagen, the fibrous protein found in connective tissue, bone and cartilage. A variety of proteolytic enzymes, or proteases, may be used to accomplish enzymatic hydrolysis of animal protein. Pepsin and trypsin are widely used for animal peptone manufacture. Pepsin is isolated from porcine or other animal stomach. Trypsin, along with chymotrypsin, carboxypeptidase A, carboxypeptidase B, and elastase, are enzymes isolated from animal pancreas.
From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Neopeptone is an enzymatic digest of protein. Neopeptone contains a wide variety of peptide sizes in combination with vitamins, nucleotides and minerals. Ash content is 6.9% NaCl content is 1.4%
An enzymatic digest of meat, used for the culturing of microorganisms.
An enzymatic digest of animal (mammalian) protein (of unspecified orgin), used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised).: Bacto™ Peptone is an enzymatic digest of animal protein. Bacto Peptone was first introduced in 1914 and became the standard Peptone for the preparation of bacteriological culture media. The nutritive value of Bacto Peptone is largely dependent on the amino acid content that supplies essential nitrogen. Bacto Peptone contains only a negligible quantity of proteoses and more complex constituents. Ash content is 6.9% NaCl content is 1.7%
A mixture of peptones (pancreatic digest of casein and peptic digest of animal/mammalian tissue - meat), used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised).: BBL™ Polypeptone™ Peptone is a mixture of peptones made up of equal parts of pancreatic digest of casein and peptic digest of animal tissue. Polypeptone Peptone includes the high content of amino acids and small polypeptides characteristic of pancreatic digest of casein and the larger polypeptides characteristic of peptic digest of animal tissue. Ash content is 9.7% NaCl content is 2.7%
From BD Bionutrients Technical Manual (3rd edition revised). The Bacto™ Proteose Peptones are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters led to the development of Proteose Peptone, Proteose Peptone No. 2 and Proteose Peptone No. 3. Data accumulated during these studies demonstrated that no one peptone is the most suitable nitrogen source for every microbiological application. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. BiTek™ Proteose Peptone and BiTek Proteose Peptone No. 3 are enzymatic digests of protein, developed to offer alternatives to the Bacto Proteose Peptones for scale-up to production applications. Ash content is 7.8% NaCl content is 4.9%
A peptic digest of protein derived from animal (mammalian) tissue, used for the culturing of microorganisms.
A mixed enzymatic digest of flour made of the seed of the soybean (Hordeum vulgare), used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised): Enzymeatic digests of soy flour. Soy contains several heat labile protease inhibitors. The most common way of eliminating these factors is to heat or toast the defatted soy beans in a processing plant under controlled conditions. Soy flour, the principle substrate in a soy peptone, is rich in high-quality protein, carbohydrates, calcium and B vitamins. The enzymes used in the digestion of soy flour are typically from animal-free sources or from microorganisms that have been grown in animal-free media. Ash content is 12% NaCl content is 0.2%
From: Wikipedia:Tryptone Tryptone is the assortment of peptides formed by the digestion of casein by the protease trypsin.
An enzymatic hydrolysate of casein (a milk protein from cow's milk, Bos taurus) made using the hydrolytic enzyme trypsin, used for the culturing of microorganisms.
From BD Bionutrients(TM) Techical Manual (3rd edition, revised): Bacto™ Tryptone is a pancreatic digest of casein. It was developed by Difco Laboratories while investigating a peptone particularly suitable for the elaboration of indole by bacteria. It is also notable for the absence of detectable levels of carbohydrates. Ash content is 6.6% NaCl content is 0.0%
From BD Bionutrients Technical Manual (3rd edition revised).: Bacto™ Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties. The digestive process of Tryptose results in assorted peptides of higher molecular weight suitable for long-chain amino acid requirements. Ash content is 8.8% NaCl content is 3.2%
A mixed enzymatic hydrolysate of protein (animal meat of unspecified origin), used for the cultivation of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Casamino Acids is an acid hydrolysate of casein, prepared according to the method described by Mueller and Miller. The method described, reduces the sodium chloride and iron content of the hydrolyzed casein. This hydrolyzed casein, supplemented with inorganic salts, growth factors, cystine, maltose and an optimum amount of iron was used by Mueller and Miller to prepare diptheria toxin. Bacto Casamino Acids duplicate this specially treated hydrolyzed casein. Ash content is 18.3% NaCl content is 12.1%
An acid hydrolysate of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms.
A complex mixture of unrefined petrolium hydrocarbons, pumped from subsurface geologic formations, used in the cultivation of microorganisms.
From Wikipedia: Yolk: The yolk of one large egg (50 g total, 17 g yolk) contains approximately: 2.7 g protein, 210 mg cholesterol, 0.61 g carbohydrates, and 4.51 g total fat. All of the fat-soluble vitamins (A, D, E, and K) are found in the egg yolk. Egg yolk is one of the few foods naturally containing vitamin D. The composition (by weight) of the most prevalent fatty acids in egg yolk is typically as follows: Unsaturated fatty acids: Oleic acid, 47% Linoleic acid, 16% Palmitoleic acid, 5% Linolenic acid, 2% Saturated fatty acids: Palmitic acid, 23% Stearic acid, 4% Myristic acid, 1%
Oil extracted from yolk of chicken eggs, used in the cultivation of microorganisms.
The process by which thiosulfate undergoes disproportionation to sulfate and hydrogen sulfide.
Oil extracted from olives (the fruit of Olea europaea), used in the cultivation of microorganisms.
From Wikipedia:Olive_oil: Olive oil is a fat obtained from the olive (the fruit of Olea europaea; family Oleaceae), a traditional tree crop of the Mediterranean Basin. The oil is produced by pressing whole olives. Olive oil is composed mainly of the mixed triglyceride esters of oleic acid and palmitic acid and of other fatty acids, along with traces of squalene (up to 0.7%) and sterols (about 0.2% phytosterol and tocosterols).
The purpose of this assay is to determine if a microbial isolate is capable of metabolizing glutamate. This enzyme is important for the synthesis of urea and in nitrogen assimilation. Glutamate is deaminated via a hydrolysis reaction to produce 2-oxoglutarate, ammonia, and either NADH or NADPH. Activity can be assayed using colorimetric assays for NADH production. 2-oxoglutarate is also known as alpha-ketoglutarate. Activity could also be determined using a pH sensitive indicator dye. Glutamate dehydrogenase catalyzes the following reaction: L-Glutamate + H2O <-> 2-oxoglutarate + NH3 + NADH/NADHP + H+ L-Glutamate + H2O <-> alpha-ketoglutarate + NH3 + NADH/NADHP + H+
An extract made from archaeal cells, used for culturing microorganisms.
An extract made from bacterial cells, used for culturing microorganisms.
From http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM1032&cat=&sec=1: Heart Infusion Broth has been developed to give the same performance characteristics as Brain Heart Infusion (BHI) Broth. However, as bovine brain is a specified risk material, the exclusion of it from the Heart Infusion Broth means that the regulatory requirements when using it are lower. The medium has been developed to give equivalent performance to Brain Heart Infusion but the exclusion of calf brain infusion means that Heart Infusion Broth carries a lower regulatory burden. Simple additions may be made to the medium to make it suitable for the cultivation of yeasts and moulds and for use in blood culture. A highly nutritious infusion medium recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms.
A water extract of bovine (Bos taurus) heart, used in the cultivation of microorganisms.
From Wikipedia:Brain_heart_infusion_broth: Brain-heart infusion broth (BHI broth or BHIB) is a highly nutritious general-purpose growth medium for culturing fastidious and nonfastidious microorganisms, such as streptococci, pneumococci and meningococci. It is made by boiling cow or porcine hearts and brains. Boiling releases soluble factors into the broth. The broth can then be turned into powder for easy distribution. BHI broth contains sodium chloride which is used to differentiate enterococci from nonenterococcal group D streptococci. BHI broth is often used in food safety, water safety, and antibiotic sensitivity tests.
Hot water extract of bovine (Bos taurus) or porcine (Sus scrofa) hearts and brains, used in the cultivation of microorganisms.
An extract made from cellular paste from the culture of Chlorella spp., used for the culturing of microorganisms. Chlorella is a microscopic green alga in the Trebouxiophyceae.
From "Materials and Methods in the Study of Protozoa", pg. 21, by Harold Kirby, University of California Press, 1950: "One part of caecal contents is mixed with 9 parts of Ringer solution, and the mixture strained through a sieve and then through a funnel with a thick pad of absorbent cotten."
A water extract of fecal material derived from some animal (mammal), used in the cultivation of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Malt Extract is the water-soluble portion of malted barley. The extraction process breaks down the polysaccharides into simple sugars. After the malting process is complete, the extract is prepared from the malted barley by cracking the grain in a mill and then extracting the grain with a warm liquor. The resulting “wort” is filtered and evaporated or dried under vacuum. Ash content is 0.3% NaCl content is 0.2%
Water-soluble extract of malted (i.e. germinated) seeds of barley (Hordeum vulgare), used for the culturing of microorganisms. Prepared by malting the seed, cracking it (grinding) using a mill, heating it in water, then filtering the extract and drying it.
A water extract of horse (Equus caballus) manure, used for the culturing of microorganisms.
From "Report of the New York State College of Agriculture at Cornell University, Ithaca, and of the Cornell University Agricultural Experiment Station", v. 25, Part 1, 1913, pg. 431: Manure extract agar. - Prepared as follows: To 100 grams of well-rotted horse manure was added 500 cc. of distilled water. This was allowed to stand for twenty-four hours at room temperature and then filtered. The filtrate was used as a stock solution.
A water extract of animal (mammalian) meat, used for the culturing of microorganisms.
From Wikipedia:Meat_extract: Meat extract is highly concentrated meat stock, usually made from beef.
From Jeaon, J-R & Kim, J-Y. 2006. Effects of pine needle extract on differentiation of 3T3-L1 preadipocytes and obesity in high-fat diet fed rats. Biol. Pharm. Bull. 29(10):2111-2115: Pine needles were collected from Kyungbook Province, Republic of Korea, and were extracted using 20 kg of water for 2 h at 80 °C. The extract was filtered, and was then freeze-dried at 40 °C. Pine needle water extract (PNE) was stored at 4 °C until use.
A hot water extract of pine needles (the adult leaves of Pinus spp.), used for the culturing of microorganisms. Formed as result of heating pine needles in warm water, filtering the extract and then drying it.
From Roubos-van den Hil, Dalmas, Nout & Abee. 2009. Soya bean tempe extracts show antibacterial activity against Bacillus cerus cells and spores. J. Appl. MIcrobiol. 109(1):137-145: Fermented soya beans (tempe) and cooked soya beans, were freeze-dried and grounded passing through a 0·5-mm sieve (Ultra Centrifugal Mill ZM 200, Retsch GmbH, Haan, Germany) and were stored at −20°C until further processing. Freeze-dried products were suspended in distilled water (60 g l−1) and stirred with a magnetic stirrer for 3 h at room temperature. The pH was continually checked and adjusted to pH 8·0 with 1 mol l−1 NaOH. To obtain clear supernatants, the soluble extract was obtained by three consecutive centrifugation steps (10 min, 10 000 g, 20°C). Supernatants were freeze-dried and soluble dry matter was stored at −20°C and used as soya bean soluble dry matter in experiments.
A water extract of ground beans (the seed) of soya (Glycine max), used for the culturing of microorganisms. Formed by fermenting the beans, heating (cooking) them, filtering the material and freeze dyring it. Next, the material is hydrated, treated with sodium hydroxide, centrifuged and freeze-dried.
From www.SigmaAldrich.com: A water soluble extract of autolyzed yeast cells. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.
A water-soluble extract of autolyzed yeast (Saccharomyces cerevisiae), used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised): Bacto™ Yeast Extract is one of the most complete and versatile fermentation bionutrients available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia sp., can be isolated from yeast extract Ash content is 11.2% NaCl content is 0.1%
A water extract of beef (Bos taurus) protein, used for the culturing of microorganisms.
From BD Bionutrients Technical Manual (3rd edition revised).: Beef Extract is derived from infusion of beef and provides an undefined source of nutrients. Beef Extract is not exposed to the harsh treatment used for protein hydrolysis, so it can provide some of the nutrients lost during peptone manufacture. Beef Extract is a mixture of peptides and amino acids, nucleotide fractions, organic acids, minerals and some vitamins. “Its function can therefore be described as complementing the nutritive properties of peptone by contributing minerals, phosphates, energy sources and those essential factors missing from peptone.” Beef Extract Powder is a meat extract dried to powder form. Bacto™ Beef Extract, Desiccated, is the dried form of Beef Extract paste. Ash content is 9.3% NaCl content is 0.3%
Cell staining assay where a cell staining assay, performed by staining a smear of prokaryotic cells with two stains (Crystal Violet or Safranin). Delineates prokaryotes into two major groups (Gram positive and Gram negative, although results can be ambiguous in some taxa) that results from the different staining of cell wall types.
Wikipedia:Gram_staining Gram staining, also called Gram's method, is a method of differentiating bacterial species into two large groups (gram-positive and gram-negative). The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting peptidoglycan, which is present in a thick layer in gram-positive bacteria.[1] In a Gram stain test, gram-positive bacteria retain the crystal violet dye, while a counterstain (commonly safranin or fuchsine) added after the crystal violet gives all Gram-negative bacteria a red or pink coloring.
zzzz has_participant some [ChEBI] safranin - Safranin is not in ChEBI yet.
morphologically distinct prokaryotic cell
A prokaryotic cell, or group of cells, having a distinct morphology (shape or size).
Physical object quality of a prokaryotic colony.
An organic-rich, liquid microbiological culture medium that contains peptones, three sugars (glucose, lactose, and sucrose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color.
From: http://www.microbelibrary.org/component/resource/laboratory-test/2842-triple-sugar-iron-agar-protocols RECIPE Pancreatic digest of casein USP (see Note) 10.0 g Peptic digest of animal tissue USP (see Note) 10.0 g Glucose 1.0 g Lactose 10.0 g Sucrose 10.0 g Ferrous sulfate or ferrous ammonium sulfate 0.2 g NaCl 5.0 g Sodium thiosulfate 0.3 g Phenol red 0.024 g Agar 13.0 g Distilled water 1,000 mL Note: The following combination of ingredients can substitute for the first two components listed: beef extract, 3.0 g; yeast extract, 3.0 g; and peptone, 20.0 g. Combine ingredients, and adjust the pH to 7.3. Boil to dissolve the agar, and dispense into tubes. Sterilize by autoclaving at 121°C for 15 min. Cool in a slanted position to give a 2.5-cm butt and a 3.8-cm slant.
An organic-rich, solid microbiological culture medium that contains peptones, three sugars (glucose, lactose, and sucrose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color.
From:http://www.microbelibrary.org/component/resource/laboratory-test/2842-triple-sugar-iron-agar-protocols RECIPE Pancreatic digest of casein USP (see Note) 10.0 g Peptic digest of animal tissue USP (see Note) 10.0 g Glucose 1.0 g Lactose 10.0 g Sucrose 10.0 g Ferrous sulfate or ferrous ammonium sulfate 0.2 g NaCl 5.0 g Sodium thiosulfate 0.3 g Phenol red 0.024 g Agar 13.0 g Distilled water 1,000 mL Note: The following combination of ingredients can substitute for the first two components listed: beef extract, 3.0 g; yeast extract, 3.0 g; and peptone, 20.0 g. Combine ingredients, and adjust the pH to 7.3. Boil to dissolve the agar, and dispense into tubes. Sterilize by autoclaving at 121°C for 15 min. Cool in a slanted position to give a 2.5-cm butt and a 3.8-cm slant.
An optical quality assay of a single prokaryotic cell, determined by visualization of a liquid culture of a prokaryotic microorganism (i.e., a clonal liquid suspension in a liquid culture medium).
A prokaryotic cell that is pigmented (in that it has a color imparted by a chemical pigment compound).
An aminopeptidase activity which cleaves the chromogenic substrate N-benzoyl-L-leucine-beta-naphthylamide.
The optical quality of a liquid culture of clonal prokaryotic organisms, which exhibits opalescent turbidity (refracting different wavelengths of light under white light illumination, or iridescence) after gentle shaking.
From: http://www.atcc.org/~/media/DF06C418BB3A44189AD1A3CEB59B8D6E.ashx ATCC Medium: 1776 Tyrosine Agar (ISP Medium 7) Glycerol 15.0 g L-Tyrosine 0.5 g L-Asparagine 1.0 g K2HPO4 0.5 g MgSO4x7H2O 0.5 g NaCl 0.5 g FeSO4x7H2O 10.0 mg Trace elemetns solution Ho-Le (see below) 1.0 mL Agar 20.0 g Distilled water 1.0 L Adjust medium for final pH 7.3 +/- 0.1. Autoclave at 121C for 15 minutes. Trace Elements Solution Ho-Le: H3BO3 2.85 g MnCl2x4H2O 1.8g FeSO4 1.36 g Sodium tartrate 1.77 g CuCl2x2H2O 26.9 mg ZnCl2 20.8 mg CoCl2x6H2O 40.4 mg Na2MoO4x2H2O 25.2 mg Distilled water 1.0 L
An organic-rich, mineral-salts, solid microbiological culture medium containing glycerol, tyrosine, asparagine, and minerals salts. Used for the growth of Streptoalloteichus.
From Wikipedia:endospore: An endospore is a dormant, tough, and non-reproductive structure produced by certain bacteria from the Firmicute phylum. The name "endospore" is suggestive of a spore or seed-like form (endo means within), but it is not a true spore (i.e., not an offspring). It is a stripped-down, dormant form to which the bacterium can reduce itself. Endospore formation is usually triggered by a lack of nutrients, and usually occurs in Gram-positive bacteria. In endospore formation, the bacterium divides within its cell wall. One side then engulfs the other. Endospores enable bacteria to lie dormant for extended periods, even centuries. Revival of spores millions of years old has been claimed. When the environment becomes more favorable, the endospore can reactivate itself to the vegetative state. Most types of bacteria cannot change to the endospore form. Examples of bacteria that can form endospores include Bacillus and Clostridium.
A differentiated prokaryotic cell, found in the Firmicutes, which is specialized for extreme tolerance to desiccation, radiation, and exposure to ultraviolet light. Characterized by thick cell walls.
Wikipedia:bacterial_cell_structure Gas vacuoles are membrane-bound, spindle-shaped vesicles, found in some planktonic bacteria and Cyanobacteria, that provides buoyancy to these cells by decreasing their overall cell density. Positive buoyancy is needed to keep the cells in the upper reaches of the water column, so that they can continue to perform photosynthesis. They are made up of a shell of protein that has a highly hydrophobic inner surface, making it impermeable to water (and stopping water vapour from condensing inside) but permeable to most gases. Because the gas vesicle is a hollow cylinder, it is liable to collapse when the surrounding pressure becomes too great.
An intracellular non-membran-bound organelle (cytoplasmic vesicle) made of protein which functions to provide buoyancy to the cell in the water column.
Cytoplasmic part which forms a distinctive granular particle, which may or may not be visible using light microscopy.
Wikipedia:granule_(cell biology) In cell biology, a granule is a small particle. It can be any structure barely visible by light microscopy.
Wikipedia:magnetosome Magnetosome chains are membranous prokaryotic structures present in magnetotactic bacteria. They contain 15 to 20 magnetite crystals that together act like a compass needle to orient magnetotactic bacteria in geomagnetic fields, thereby simplifying their search for their preferred microaerophilic environments. Each magnetite crystal within a magnetosome is surrounded by a lipid bilayer, and specific soluble and transmembrane proteins are sorted to the membrane. Recent research has shown that magnetosomes are invaginations of the inner membrane and not freestanding vesicles. Magnetite-bearing magnetosomes have also been found in eukaryotic magnetotactic algae, with each cell containing several thousand crystals.
Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes.
An isopolar trichome morphology quality where heterocysts are rarely present along the length of the trichome such that the trichome morphology is asymmetric.
From:Humphry DR, George, A, Black GW, Cumming& s SP. 2001. Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. IJSEM 51:1235-1243. Minimal medium (MM) containing the following (w/v): 0.02% FeSO4, 0.02% MgSO4, 0.075% KNO3, 0.05% K2HPO4 and 0.004% CaCl2. The pH was adjusted to 7.2 with 1 M NaOH or 1M HCl.
A mineral-salts, liquid microbiological culture medium comprised of ferrous sulfate, magnesium sulfate, potassium nitrate, potassium phosphate, and calcium chloride, supplemented with 0.5% w/v xylose. Used for the cultivation of Flavobacterium frigidarium.
Thylakoid-containing cell where thylakoids are dispered irregularly (asymmetrically) throughout the interior of the cell.
Thylakoid-containing cell where thylakoids are located around the inside periphery of the interior of the cell.
Thylakoid-containing cell where thylakoids are distributed throughout the inside of the cell and have a radial (like spokes on a wheel) arrangement.
Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals made of greigite that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes.
Wikipedia:magnetosome Magnetotactic bacteria usually mineralize either iron oxide magnetosomes, which contain crystals of magnetite (Fe3O4), or iron sulfide magnetosomes, which contain crystals of greigite (Fe3S4).
Intracellular membrane-bound organelle that form chains, comprised of magnetic iron minerals made of magnetite that function to orient the cell axis along the Earth's magnetic field lines. Found in magnetotactic prokaryotes.
A prokaryotic cell which has intracellular granules.
A prokaryotic cell part quality that has gas vacuoles (gas vesicles).
An intracellular granule that is specialized for the storage of carbon.
An intracellular granule that is specialized for the storage of nitrogen.
intracellular phosphorus storage granule
Intracellular granule composed of elemental sulfur.
Wikipedia:Chromatiaceae The Chromatiaceae are the main family of purple sulfur bacteria. Many members conduct an anoxygenic photosynthesis. They are distinguished from the Ectothiorhodospiraceae by producing sulfur globules and storing them within their cells.
cell having small gas vacuole
A gas vacuolated cell, were gas vacuoles are small (decreased) in size.
cell having large gas vacuole
A gas vacuolated cell, were gas vacuoles are large (increased) in size.
A prokaryotic cell that has gas vacuoles (gas vesicles).
Intracellular phosphorus storage granule composed of polyphosphate.
Wikipedia:volutin_granules Volutin granules are an intracytoplasmic (inside the cytoplasm of a cell) storage form of complexed inorganic polyphosphate, the production of which is used as one of the identifying criteria when attempting to isolate Corynebacterium diphtheriae on Löffler's medium. Polyphosphate granules are called metachromatic granules due to their displaying the metachromatic effect; they appear red or blue when stained with the blue dyes methylene blue or toluidine blue.
Wikipedia:cyanophycin Cyanophycin, or multi-L-arginyl-poly (L-aspartic acid), is a non-protein, non-ribosomally produced amino acid polymer composed of an aspartic acid backbone and arginine side groups.
Intracellular carbon and nitrogen storage granule composed of cyanophycin macromolecules.
Filippini M, Svercel M, Laczko E, Kaech A, Ziegler U & Bagheri HC. 2011. Fibrella aestuarina gen. nov., sp. nov., a filamentous bacterium of the family Cytophagaceae isolated from a tidal flat, and emended description of the genus Rudanella Weon et al. 2008. IJSEM 61:184-189. 0.5% yeast water
An organic-rich, liquid microbiological culture medium consisting of a solution of yeast extract (0.5%) in water.
From: Filippini M, Kaech A, Ziegler U & Bagheri HC. 2011. Fibrisoma limi gen. nov., sp. nov., a filamentous bacterium isolated from tidal flats. IJSEM 61:1418-1424. 0.5% yeast water
Wikipedia:polyhydroxyalkanoates Polyhydroxyalkanoates or PHAs are linear polyesters produced in nature by bacterial fermentation of sugar or lipids. They are produced by the bacteria to store carbon and energy. More than 150 different monomers can be combined within this family to give materials with extremely different properties. These plastics are biodegradeable and are used in the production of bioplastics.
Intracellular carbon storage granule composed of poly-beta-hydroxyalkanoate.
Intracellular carbon storage granule composed of poly-beta-hydroxybutyrate.
Wikipedia:polyhydroxybutyrate Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate (PHA), a polymer belonging to the polyesters class that are of interest as bio-derived and biodegradable plastics. The poly-3-hydroxybutyrate (P3HB) form of PHB is probably the most common type of polyhydroxyalkanoate, but other polymers of this class are produced by a variety of organisms: these include poly-4-hydroxybutyrate (P4HB), polyhydroxyvalerate (PHV), polyhydroxyhexanoate (PHH), polyhydroxyoctanoate (PHO) and their copolymers.
Intracellular carbon storage granule composed of starch.
Granulated cell, where the granual size is coarse (has an increased size).
Granulated cell, where the granule size is fine (has a decreased size).
Granulated cell, where there are many granules (an increased number) per cell.
Granulated cell, where there are one granule per cell.
An endospore that forms in the middle of the cell.
An eEndospore that forms laterally to the long axis of a cell.
An endospore that forms near the ends (or the poles) of the cell, but not at the extreme ends of the cell.
An endospore that forms at the pole (end) of the cell.
Amorphously described generic term for filament- or pili-like structures.
A differentiated prokaryotic cell, found in the Nostocales (Cyanobacteria), which play a role in dormancy (such as overwintering in lake sediments). Characterized by thick cell walls.
From Wikipedia:akinete: An akinete is a thick-walled dormant cell derived from the enlargement of a vegetative cell. It serves as a survival structure. It is a resting cell of cyanobacteria and unicellular and filamentous green algae. Under magnification, akinetes appear thick walled with granular-looking cytoplasms.
Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563.
An akinete which is proximally located to a heterocyst.
An akinete that isdistally located from the heterocytes. May occur multiply in chains that, in the absence of combined nitrogen, are initiated at a site that is equidistant from two intercalary or terminal heterocysts.
An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, yeast extract, and sodium chloride. Used for the cultivation of Escherichia coli.
From: 2x Yeast Extract Tryptone (2X YT) Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use 2x YT Medium is used for cultivating recombinant strains of Escherichia coli. Principles of the Procedure Peptone and yeast extract provide the necessary nutrients and cofactors required for excellent growth of E. coli. Sodium chloride is included to provide a suitable osmotic environment. 2x YT Medium formula per Liter: Pancreatic digest of casein 16.0 g Yeast extract 10.0 g Sodium chloride 5.0 g pH 7.0 ± 0.2
An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, and sodium chloride. Used for the cultivation of Escherichia coli.
From: 2x Yeast Extract Tryptone (2X YT) Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use 2x YT Medium is used for cultivating recombinant strains of Escherichia coli. Principles of the Procedure Peptone and yeast extract provide the necessary nutrients and cofactors required for excellent growth of E. coli. Sodium chloride is included to provide a suitable osmotic environment. 2x YT Medium formula per Liter: Pancreatic digest of casein 16.0 g Yeast extract 10.0 g Sodium chloride 5.0 g Agar pH 7.0 ± 0.2
From: http://www.neogen.com/Acumedia/pdf/ProdInfo/7161_PI.pdf Intended Use Todd Hewitt Browth is used for the cultivation of steptococci and other fastidious microorganisms. Formula / Liter: Heart infusion (dehydrate) 3.1 g Yeast enriched peptone 20 g Dextrose 2 g Sodium chloride 2 g Disodium phosphate 0.4 g Sodium carbonate 2.5 g Final pH: 7.8 +/- 0.2 at 25˚C. Directions: 1. Dissolve 30 g of the medium in one liter of purified water. 2. Heat with frequent agitation to compeltely dissolve the medium. 3. Autoclave at 121 ˚C for 15 minutes.
An organic-rich, liquid microbiological culture medium containing heart infusion (beef heart infusion), yeast enriched peptone (Biosate peptone), dextrose (D-glucose), sodium chloride, disodium hydrogenphosphate, and sodium carbonate. Used for the cultivation of Streptococci.
Castenholz, 2001, in Bergey's Manual of Systematic Bacteriology, vol 1, 2nd ed., pg. 474. An "envelope" outside of the outer membrane.... variously called the sheath, glycocalyx, or capsule, or depending on the consistency, may be referred to as gel, mucilage, or slime. Many sheaths show a microfibrillar substructure. The sheaths of cyanobacteria are predominantly composed of polysaccharide, but in some strains >20% of the weight may consist of polypeptides. ... pigments may accumulate and mask the color of the cells.
Part of the glycocalyx, or envelope of a prokaryotic cell, that lies outside the outer membrane.
A sheath having a layered, laminated (i.e., laminar) structure.
A sheath that is pigmented (contains pigmented compounds).
From: Tryptone Glucose Extract Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptone Glucose Extract Agar is used for cultivating and enumerating microorganisms in water and dairy products. Principles of the Procedure Nutrients, including amino acids, carbon compounds, carbohydrates, minerals and trace substances, are supplied by the tryptone, beef extract and dextrose. Agar is the solidifying agent in Tryptone Glucose Extract Agar. Formulae Difco™ Tryptone Glucose Extract Agar Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Tryptone...................................................................... 5.0 g Dextrose (Glucose)....................................................... 1.0 g Agar.......................................................................... 15.0 g pH 7.0 ± 0.2 Difco™ m TGE Broth Approximate Formula* Per Liter Beef Extract.................................................................. 6.0 g Tryptone.................................................................... 10.0 g Dextrose (Glucose)....................................................... 2.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, solid microbiological culture medium containing beef extract, tryptone, and glucose. Used for cultivation of microorganisms from water and dairy products.
Wikipedia:pseudopeptidoglycan Pseudopeptidoglycan (also known as pseudomurein) is a major cell wall component of some archaea that differs from bacterial peptidoglycan in chemical structure, but resembles eubacterial peptidoglycan in morphology, function, and physical structure. The basic components are N-acetylglucosamine and N-acetyltalosaminuronic acid (peptidoglycan has N-acetylmuramic acid instead), which are linked by β-1,3-glycosidic bonds. Lysozyme, a host defense mechanism, is ineffective against organisms with pseudopeptidoglycan cell walls. Lysozyme can break β-1,4-glycosidic bonds to degrade peptidoglycan; however, pseudopeptidoglycan has β-1,3-glycosidic bonds, rendering lysozyme useless.
Cell wall composed a polymer of N-acetylglucosamine and N-acetyltalosaminuronic acid (2-amino-2-deoxy-L-taluronic acid), linked by beta-1,3-glycosidic bonds. Found in the archaeal domain of life.
From BD Bionutrients Technical Manual (3rd edition revised): BBL™ Biosate™ Peptone is a mixed hydrolysate comprised of 65% pancreatic digest of casein and 35% yeast extract. Ash content is 7.7% NaCl content is 0.3%
A mixed hydrolysate of pancreatic digest of casein (65%) and yeast extract (35%). Biosate is a Trademark of BD.
Wikipedia:teichoic acid Teichoic acids (cf. Greek τεῖχος, teīkhos, "wall", more specifically a fortification wall, as opposed to τοῖχος, toīkhos, a regular wall) are bacterial polysaccharides of glycerol phosphate or ribitol phosphate linked via phosphodiester bonds. Teichoic acids are found within the cell wall of Gram-positive bacteria such as species in the genera Staphylococcus, Streptococcus, Bacillus, Clostridium, Corynebacterium and Listeria, and appear to extend to the surface of the peptidoglycan layer. Teichoic acids are not found in Gram-negative bacteria. They can be covalently linked to N-acetylmuramic acid of the peptidoglycan layer, to the lipids of the cytoplasmic membrane, or to a terminal D-alanine in the tetrapeptide crosslinkage between N-acetylmuramic acid units. Teichoic acids that remain anchored to lipids are referred to as lipoteichoic acids, while teichoic acids that are covalently bound to peptidoglycan are referred to as wall teichoic acids.
Cell wall composed of polysaccharides of glycerol phosphate or ribitol phosphate linked via phosphodiester bonds, found in the peptidoglycan cell walls of Gram-positive bacteria.
An organic-rich, liquid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood, yeast extract, bacitracin, and polymyxin B. Used for the cultivation of Capnocytophaga spp.
From: Mashimo PA, Yamamoto Y, Nakamura M & Slots J. 1983. Selective recovery of oral Capnocytophaga spp. with sheep blood agar containing bacitracin and polymyxin B. J Clin Microbiol 17:187-91. TBBP medium consists of 4% Trypticase soy agar (BBL Microbiology Systems, Coceysville, Md.), 5% sheep blood, 0.1 % yeast extract, 50 micrograms of bacitracin per mL, and 100 micrograms of polymyxin B per mL.
An organic-rich, solid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood, yeast extract, bacitracin, and polymyxin B. Used for the cultivation of Capnocytophaga spp.
A prokaryotic differentiated cell capable of nitrogen fixation, found in Trichodesmium species (Cyanobacteria). Diazocytes are mostly found in the center cells of Trichodesmium filaments.
From Sandh, Xu & Bergman, 2012, Diazocyte development in the marine diazotrophic cyanobacterium Trichodesmium. Microbiology 158:345-352.: The establishment of non-diazotrophic cultures of the filamentous marine cyanobacterium Trichodesmium erythraeum IMS101 enabled the first detailed investigation of the process leading to the development of its unique nitrogen-fixing cell type, the diazocyte.
Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates that form outside the cell as a result of the local presence of the cell or the activities of the cell.
Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates of iron minerals that form outside the cell as a result of the local presence of the cell or the activities of the cell.
Crystalline, microcystalline, nanocrystalline, or amorphous inorganic chemical precipitates of elemental sulfur that forms outside the cell as a result of the local presence of the cell or the activities of the cell. Produced by Ectothiorhodospiraceae.
Wikipedia:Ectothiorhodospiraceae The Ectothiorhodospiraceae are a family of purple sulfur bacteria, distinguished by producing sulfur globules outside of their cells.
Wikipedia:Fimbria_(bacteriology) In bacteriology, a fimbria [(plural fimbriae); also referred to as a pilus (plural pili) by some scientists] is an appendage composed of curlin proteins that can be found on many Gram-negative and some Gram-positive bacteria that is thinner and shorter than a flagellum. This appendage ranges from 3-10 nanometers in diameter and can be up to several micrometers long. Fimbriae are used by bacteria to adhere to one another and to adhere to animal cells and some inanimate objects. A bacterium can have as many as 1,000 fimbriae. Fimbriae are only visible with the use of an electron microscope. They may be straight or flexible.
A dilute organic-containing, liquid microbiological culture medium containing tryptone, yeast extract, beef extract, and acetate, in a base of half-strength artifical seawater.
From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. SWC contained (per liter of 1/2 strength artificial seawater [ASW]): tryptone, 0.5 g; yeast extract, 0.5 g; beef extract, 0.2 g; sodium acetate, 0.2 g. The pH was adjusted to 7.6 with a dilute solution of NaOH. ASW, 1/2 strength, contained (per liter): NaCl, 12.0 g; MgSO4x7H2O, 3.5 g; MgCl2x6H2O, 2.6 g; CaCl2x2H2O, 0.55 g; KCl, 0.35 g.
An assay for the ability of a microorganism to reduce a compound that contains a tetrazoleum moiety. Tetrazolium compounds are redox indicators of microbial oxidation and reduction, and hence microbial respiration.
Flagellated cell where the cell has more than one flagellum.
Prokaryotic cell which has either a bacterial-type flagellum (or flagella) or a periplasmic flagellum.
Flagellated cell where the cell has only one flagellum.
http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Monotrichous: Single polar flagellum e.g. Vibrio cholerae (Mneomonics: Mono means one)
Flagellated cell where the cell has a single flagellum located at the end (pole) of the cell.
Flagellated cell where the cell has two flagella, each located at the ends (poles) of the cell.
http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Amphitrichous: Single flagellum at both ends e.g. Alcaligenes faecalis (Mneomonics: Remember: the characteristics of Amphibians: live both in land and water)
Flagellated cell where the cell has multiple flagella, located in a tuft at the end (pole) of the cell.
http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Lophotrichous: Tuft of flagella at one or both ends e.g. Spirilla
Flagellated cell where the cell has multiple flagella, each located in tufts in the periplasmic space.
http://microbewiki.kenyon.edu/index.php/Spirochaeta The morphology and cellular structure of Spirochaeta spp. (and most other Spirochetes) is unique among prokaryotes. The cells are helical in shape and consist of an outer membrane, axial filaments (ultrastructurally similar to bacterial flagella), and a protoplasmic cylinder. The outer membrane or the outer sheath surrounds all of the structures including the axial filaments and the protoplasmic cylinder. The protoplasmic cylinder is the cell body of the organism. It is coiled and is composed of the cytoplasm, the nuclear region and the peptidoglycan-cytoplasmic membrane complex. The axial filaments are usually called the periplasmic flagella but are also known as periplasmic fibrils, axial fibrils and endoflagella.
http://microbeonline.com/bacterial-flagella-structure-importance-and-examples-of-flagellated-bacteria/ Peritrichous: Flagella surrounding the cell, e.g. Typhoid bacilli (Mneomonic: Remember Periphery)
Flagellated cell where the cell has multiple flagella, all located at the periphery of the cell.
Prokaryotic motility that is characterized microscopically by end-over-end tumbling and employs a flagellum. This type of motility is typical of Listeria.
From Wikipedia:heterocyst: Heterocysts are specialized nitrogen-fixing cells formed during nitrogen starvation by some filamentous cyanobacteria, such as Nostoc punctiforme, Cylindrospermum stagnale, and Anabaena sphaerica. They fix nitrogen from dinitrogen (N2) in the air using the enzyme nitrogenase, in order to provide the cells in the filament with nitrogen for biosynthesis. Nitrogenase is inactivated by oxygen, so the heterocyst must create a microanaerobic environment.
A prokaryotic differentiated cell capable of nitrogen fixation, found in the filamentous Nostocales (Cyanobacteria). Heterocytes (which differentiate into heterocysts) have thick cell walls which exclude oxygen in the air and allow nitrogen fixation to occur. Are terminally differentiated cells, incapable of carrying out photosynthesis.
A heterocyte which develops at the middle or near the end (but not the end) of a filament (trichome).
From Rippka, Castenholz & Herdman, 2001, in Bergeys Manual of Systematic Bacteriology, v. 1, 2nd ed., pg 563.: In the absence of combined nitrogen (ammonium or nitrate), 5-10% of the vegetative cells differentiate into heterocysts. These specialized cells are the sites of aerobic nitrogen fixation and may occupy terminal or intercalary positions in the trichomes.
A heterocyte which develops at the end of a filament (trichome).
Prokaryotic differentiated cell specialized for asexual reproduction comprised of short chains of motile filaments, found in the Nostocales, Stigonematales/Stigonemataceae, and Oscillatoriales (Cyanobacteria). Hormogonia are shorter than the typical length of vegetative filaments.
From Wikipedia:hormogonium: Hormogonia are motile filaments of cells formed by some cyanobacteria in the order Nostocales and Stigonematales. They are formed during asexual reproduction in unicellular, filamentous cyanobacteria some contain heterocysts and akinetes.
A dilute organic-containing, liquid microbiological culture medium containing tryptone, yeast extract, beef extract, and acetate, in a base of full-strength artifical seawater.
From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. SWC-m contained (per liter of full strength ASW): KH2PO4, 0.01 g; ferric citrate, 0.001 g; NH4Cl, 0.4 g; yeast extract, 0.4 g; beef extract, 0.4 g; tryptone, 0.4 g; vitamins, 10 mL; trace elements solution (TES), 2.0 mL; carbon source, 0.2 g; pH 7.0. The following macromolecular carbon sources were tested for hydrolysis on plates: starch, 1.0%; tributyrin, 1.0%; chitin, 1.0%; casein, 0.5%. xxxxxxx From: Irgens RL. 1977. Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27(1):38-43. The TES, modified from Pfennig’s formula (personal communication), contained (per liter): ZnSO4x7H2O, 0.10 g; MnCl2x4H2O, 0.03 g; H3BO3, 0.3 g; CoCl2x6H20, 0.2 g; CuCl2x2H2O,0.01 g ; NiCl2x6H2O, 0.02 g; Na2MoO4x 2H2O, 0.03g; pH3 to 4. xxxxxxx From: Staley JT. 1981. The genera Prosthecomicrobium and Ancalomicrobium. In: Starr MP, Stolp H, Truper HG, Balows A,Schlegel HG, (eds). The prokaryotes. Berlin: Springer-Verlag. Pg 68. Vitamin Solution B12 0.1 mg Biotin 2 mg Calcium pantothenate 5 mg Folic acid 2 mg nicotinamide 5 mg pyridoxine HCl 10 mg riboflavin 5 mg thiamine HCL 5 mg Add distilled water up to 1 L and sotre in 4˚C in a dark container.
Pilus involved in the transfer of DNA from one bacterium (the donor) to another (the recipient).
Wikipedia:pilus Conjugative pili allow the transfer of DNA between bacteria, in the process of bacterial conjugation. They are sometimes called "sex pili", in analogy to sexual reproduction, because they allow for the exchange of genes via the formation of "mating pairs". Perhaps the most well-studied is the F pilus of Escherichia coli, encoded by the F plasmid or fertility factor.
From: Irgens Rl, Suzuki I, Staley JT. 1989. Gas vacuolate bacteria obtained from marine waters of Antarctica. Curr Microbiol 18:261-5. Agar slants containted 1.5 % agar, agar plates contained 2.0 % agar, and soft agar tubes contained 0.2 % agar.
A colony that has a distinct morphology manifested in its 2D or 3D shape.
A prokaryotic colony that has distinct physical characteristics (qualities).
A colony having distinct physical quality that manifests in the spatial pattern of the elements of the colony.
A prokaryotic colony that manifests in how light (visible electromagnetic radiation) passes through the colony.
A colony with a texture that manifests in the appearance of the surface characteristics.
A prokaryotic colony where small colonies surround larger colonies on an agar (or other solid) surface.
http://www.yourdictionary.com/satellite#americanheritage Satellite colonies occur when tiny colonies are found to surround a larger central colony.
A colony with distinct spatial pattern where the center part of the colony is tight (densely compacted) and the outer part of the colony is sparse (not densely compacted).
A colony having distinct physical quality that is dry (has a brittle, friable texture that breaks apart).
A colony with distinct optical quality that is dull (not shiny, with a low saturation) in its appearance.
A colony with a structure that has a grainy, or granular, appearance in its center.
A colony with distinct EM radiation quality that has a wet appearance.
A colony with a structure that is mucoid (has a sticky appearance due to the presence of exopolysaccharide capsular material).
A colony with a texture that is rough (i.e. not smooth).
A colony with a shape that has raised, branching features starting in the center of the colony, with an appearance that resembles veins or branches of a tree.
A colony with a texture that is wrinkled (has a shriveled appearance).
A colony with distinct optical quality that is cloudy (semi-translucent or semi-opaque, whitish in color).
A colony having a distinct process quality where the colony is iridescent (appears to change color as the angle of illumination changes).
A colony with a structure that contains coloration due to the presence of pigment compounds that absorb particular wavelengths of light.
A colony with distinct optical quality that is opaque (where light does not pass through).
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Bovine Albumin contains bovine albumin fraction V, 0.2% in 0.85% saline solution.
A blood medium ingredient comprised of bovine (Bos taurus) albumin (a mixture of protein derived from blood). Used in the cultivation of microorganisms.
A colony with distinct optical quality that is translucent, where much light is able to pass through it without absorption of particular wavelengths of light.
A colony with distinct optical quality that is completely or nearly completely clear or transparent, where nearly all light is able to pass through it.
anaerobic respiration, using sulfate as electron acceptor
The process of anaerobic respiration, where sulfate is reduced to sulfite, tetrathionate, thiosulfate, elemental sulfur, or hydrogen sulfide.
A hydrolase activity, which hydrolyzes pyrazinamide (a drug used to treat tuberculosis) into pyrazinoic acid (the active agent against tuberculosis). Pyrazinamidase catalyzes the following reaction: Pyrazinamide + H2O <=> pyrazinoic acid (pyrazine-2-carboxylic acid) + NH3.
anaerobic respiration, using elemental sulfur as electron acceptor
The process of anaerobic respiration, where elemental sulfur reduced to hydrogen sulfide.
aerobic respiration, using hydrogen sulfide as electron donor
The process of aerobic respiration, where hydrogen sulfide is oxidized (using oxygen) to elemental sulfur, thiosulfate, tetrathionate, sulfite, or sulfate.
aerobic respiration, using elemental sulfur as electron donor
The process of aerobic respiration, where elemental sulfur is oxidized (using oxygen) to thiosulfate, tetrathionate, sulfite, or sulfate.
anaerobic respiration, using thiosulfate as electron acceptor
The process of anaerobic respiration, where thiosulfate is reduced to elemental sulfur or hydrogen sulfide.
anaerobic respiration, using sulfite as electron acceptor
The process of anaerobic respiration, where sulfite is reduced to tetrationate, thiosulfate, elemental sulfur, or hydrogen sulfide.
Chymotrypsin assay that uses the substrate N-Benzoyl-L-tyrosine ethyl ester (BTEE). Chymotrypsin activity will cleave the substrate, releasing N-Benzoyl-L-tyrosine. The presence of this produce is measured by recording the increase in absorbance at 256 nm.
chymotrypsin assay using N-benzoyl-Phe-pNA
N-benzoyl-D-L-phenylalanine 2-naphthylamide
Chymotrypsin assay that uses the substrate N-benzoyl-D-L-phenylalanine-2-naphthylamide at pH 7.1. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
Uses the substrate N-benzoyl-D-L-arginine 2-naphthylamide at pH 8.5. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color.
An aminopeptidaseeptidase activity which cleaves the chromogenic substrate L-alanyl-L-alanine-2-naphthylamide.
alanyl phenylalanyl proline arylamidase activity
An aminopeptidase activity which cleaves the chromogenic substrate L-alanyl-L-phenylalanyl-L-proline-2-naphthylamide.
glutamyl glutamic acid arylamidase activity
An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-L-glutamic acid-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-glycine-2-naphthylamide.
glutamyl glycyl arginine arylamidase activity
An aminopeptidase activity which cleaves the chromogenic substrate L-glutamyl-glycyl-L-arginine-2-naphthylamide.
glutaryl phenylalanine arylamidase activity
An aminopeptidase activity which cleaves the chromogenic substrate glutaryl-L-phenylalanine-2-naphthylamide.
glycyl phenylalanine arylamidase activity
An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-phenylalanine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-proline-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate glycyl-L-tryptophan-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate glycyl-glycine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-leucyl-L-glycine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-seryl-L-tyrosine-2-naphthylamide.
N-carbobenzoyl-glycyl-glycyl-L-arginine peptidase activity
An aminopeptidase activity which cleaves the chromogenic substrate N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide.
Prokaryotic cell where the shape is irregular.
An optical quality assay of a single prokaryotic cell, determined by visualization under a microscope.
N-benzoyl-L-valyl-glycyl-L-arginine-4-methoxy peptidase activity
An aminopeptidase activity which cleaves the chromogenic substrate N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide.
A prokaryote colony where the shape of the colony margin is asymmetrical (i.e. irregular).
A colony with distinct spatial pattern where the colony is diffuse (sparse, where the colony is very thin).
A filamentous colony with a shape that is fuzzy, or filamentous and where the colony margin is asymmetrical (i.e. irregular).
A colony with a shape that is undulate (wave-like) and where the colony margin is asymmetrical (i.e. irregular).
A prokaryotic colony where the shape of the colony margin is symmetrical (i.e. regular).
A colony with a shape that has a curled edge, and where the colony margin is symmetrical (i.e. regular).
A colony with a shape which is circular (round), and where the colony margin is symmetrical (i.e. is regular).
A colony with a shape which is erose (i.e. has a notched, toothed, or indented appearance) and where the colony margin is symmetrical (i.e. regular).
A filamentous colony with a shape that is fuzzy, or filamentous and where the colony margin is symmetrical (i.e. regular).
A colony with a shape that is lobed and where the colony margin is asymmetrical (i.e. irregular).
A colony with a shape that is circular (round).
A colony with distinct spatial pattern where the colony is effuse (i.e. thin, unlocalized without defined margins, with a tendancy to spread).
A colony with a shape that is fuzzy, or filamentous.
Fallon RJ. 1967. Mycoplasmas and their role as rodent pathogens. Lab Anim 1:43. DOI: 10.1258/002367767781006794.
A colony having distinct physical quality that has differential density. The center part of the colony is opaque and granular, while the surrounding perifery is flat and transulcent, giving the colony the appearance of a fried egg. This shape is typical of Mycoplasma species, and is due to colonies having a thicker center than edge. This appearance is typical of older (mature) colonies of Mycoplasma.
colony having distinct electromagnetic (EM) radiation quality
A colony having distinct physical quality that manifests in the electromagnetic (EM) radiation appearance of the colony.
A colony having distinct physical quality that is shaped like a mulberry (acinar), sunken, and opaque with little or no pale periphery. Is typical of young colonies of Mycoplasma species that will develop into colonies that have a fried egg appearance when more mature.
A colony with a shape that is very tiny, like a point (where colonies may have a diameter of less than 1 mm).
A colony having distinct physical quality that is rhizoidal (has margins or edges that resemble the roots of a tree, radiating out from the center of the colony).
A colony with a shape that is spindle-shaped (i.e. is thickest in the middle, tapering to both ends).
A colony with a shape that has a depression (or crater) in the center of the colony, and thus has a 2D profile that is cup-shaped.
A colony with a shape that is flat (has a flat profile).
A colony with a shape that is raised, or elevated from the surface of the agar.
A colony with a shape that is pulvinate (is strongly elevated with a domed profile being shaped like a cushion).
A colony with a shape that has a convex, or domed, profile.
A colony having distinct physical quality that is umbonate (having a mushroom-like profile with a higher center and a lower margin).
The optical assay of a single prokaryotic cell, having the quality of autofluorescence when illuminated with a particular wavelength of light.
Autofluorescence of a prokaryotic cell that is blue-green in nature, occurring when the cells are illuminated with radiation at 420 nm. Occurs due to the presence of a particular fluorescent compound in the cell (e.g., Factor 420, also known as Coenzyme F420).
A lipase that hydrolyzes C10 compounds. Assayed using the chromogenic substrate 5-bromo-3-indoxyl-caprate (5-bromo-3-indolyl decanoate; blue-caprate).
A lipase that hydrolyzes C14 compounds. Assayed using the chromogenic substrate 2-naphthyl myristate.
A lipase that hydrolyzes C8 compounds. Assayed using the chromogenic substrate 2-naphthyl caprylate.
Wikipedia:Coccobacillus A coccobacillus (plural coccobacilli) is a type of bacterium with a shape that is intermediate between cocci (spherical bacteria) and bacilli (rod-shaped bacteria). Coccobacilli are therefore in essence very short rods which may be mistaken for cocci. Haemophilus influenzae, Gardnerella vaginalis, and Chlamydia trachomatis are coccobacilli. Aggregatibacter actinomycetemcomitans is a gram-negative coccobacillus that is prevalent in subgingival plaques. Acinetobacter strains may grow on solid media as coccobacilli. Bordetella pertussis is a gram-negative coccobacillus responsible for causing whooping cough.
A prokaryotic cell, being a bacillus where the long axis of the cell is only slightly longer than the short axis of the cell.
Wikipedia:Spirillum Spirillum in microbiology refers to a bacterium with a cell body that twists like a spiral. It is the third distinct bacterial cell shape type besides coccus and bacillus cells.
A prokaryotic cell, where the long axis of the cell twists in a spiral.
prokaryotic cell having distinct cell arrangement
Prokaryotic cells and the physical relationships between the cells (whether they are physically attached, or connected, to one another).
A multicellular relationship of prokaryotic cells where two cells are attached to one another in pairs.
morphologically differentiated filament part
A filament of prokaryotic cells, where the parts of the filament are morphologically differentiated.
A multicellular relationship of prokaryotic cells where cells are attached to one another in multiples of four (8,12, or 16). This morphology forms as a result of cell division occurring in three planes.
A multicellular relationship of prokaryotic cells where cells are attached to one another end-to-end and side-to-side to form clumps or sheets after dividing. This morphology forms as a result of cell division taking place in multiple planes.
A multicellular relationship of prokaryotic cells where cells are attached to one another end-to-end to form chains. Forms when cell division occurs in one plane and cells become detached after cell division takes place.
A multicellular prokaryotic morphological quality where cells are attached to one another in multiples of four. Forms when cell division occurs in two planes at right angles to one another.
A prokaryotic cellularity where cells are isolated and singular, where the daughter cells detach from one another after cell division and septation occurs.
Wikipedia:Streptobacillus Streptobacillus is a genus of aerobic, gram-negative facultative anaerobe bacteria, which grow in culture as rods in chains.
A multicellular relationship of prokaryotic cells where the shape of the cell is elongated (forming a bacillus, or short rod) and the cells are attached to one another end-to-end to form chains.
Wikipedia:Streptococcus Streptococcus is a genus of spherical Gram-positive bacteria belonging to the phylum Firmicutes and the lactic acid bacteria group. Cellular division occurs along a single axis in these bacteria, and thus they grow in chains or pairs, hence the name—from Greek στρεπτος streptos, meaning easily bent or twisted, like a chain (twisted chain). Contrast this with staphylococci, which divide along multiple axes and generate grape-like clusters of cells. Most streptococci are oxidase- and catalase-negative, and many are facultative anaerobes.
A multicellular relationship of prokaryotic cells where the shape of the cells is round (forming a coccus) and the cells are attached to one another end-to-end to form chains.
A multicellular relationship of prokaryotic cells where the shape of the cells is round (forming a coccus) and the cells are attached to one another end-to-end and side-to-side to form clumps or clusters.
Wikipedia:Staphylococcus Staphylococcus (from the Greek: σταφυλή, staphylē, "grape" and κόκκος, kókkos, "granule") is a genus of Gram-positive bacteria. Under the microscope, they appear round (cocci), and form in grape-like clusters.
A differentiated cyanobacterial filament where the filaments are branched.
false branching growing usually at first parallel with original trichome, later diverging
Differentiated filament branching, where filaments have "false" branches. Initiated by the interruption of a trichome between two vegetative cells, either at the location of a necridic cell or a heterocyte. Later on, one or both ends of the interrupted trichomes grow aside from the axis of the main trichome. Found in the Oscilaltoriales and Nostocales and Stigonematales.
http://www.merriam-webster.com/dictionary/false%20branching A branched arrangement of the cells of certain filamentous bacteria and algae resulting from a slipping of the end of one cell past that of another following cell division, from continued growth of the free end of a trichome through the sheath in various blue-green algae, or esp. from continued growth of parts of a filament separated by one or more intervening dead cells or by heterocysts.
pseudobranchings adpressed with each other and only in the upper parts divaricate
true-branched filaments
Differentiated filament branching, where filaments have "true" branches. Formed when one cell occasionally divides longitudinally (with the cell division plane parallel to the plane of the filament axis) or obliquely with respect to the main axis of the filament. Branches remain attached to the main trichome.
true branches grow initially perpendicular to the mother trichome
branches arise after lengthwise cell division
A multicellular prokaryotic morphological quality where the shape of the cells are elongated (i.e. bacillus or rod-shaped) and two cells are attached to one another in pairs.
http://medical-dictionary.thefreedictionary.com/diplobacillus A short, rod-shaped organism occurring in pairs, joined end-to-end; diplobacterium.
Wikipedia:Diplococcus A diplococcus (plural diplococci) is a round bacterium (a coccus) that typically occurs in the form of two joined cells. Examples are gram-negative Neisseria sp., and gram-positive Streptococcus sp. and Staphylococcus sp.. Its name comes from diplo, meaning double, and coccus, meaning berry.
A multicellular prokaryotic morphological quality where the shape of the cells are round and two cells are attached to one another in pairs.
erect filaments
A filament orientation quality where filaments are lying upright (perpendicular) to a substrate or surface.
prostrate filaments
A filament orientation quality where filaments are lying along (parallel to) a substrate or surface.
straight filament
A filament shape where the filament has a straight appearance (is not bent, curved, coiled, or torulose).
curved filament
A filament shape where the filament has a curved appearance along a gradual angle (not straight or bent).
coiled filament
A filament shape where the filament has a regularly coiled (helical) appearance.
bent filament
A filament shape where the filament has an appearance of being bent at an angle.
torulose filament
A torulose filament shape where the filament has a non-uniformly beaded appearance, where parts of the filament are swollen and constricted.
http://www.thefreedictionary.com/torulose Adj. 1. torulose - of a cylindrical or ellipsoid body; swollen and constricted at intervals
moniliform filament
A filament shape where the filament has a uniformly regular, beaded appearance.
Wikipedia: Moniliform Having a form resembling a string of beads, where the component parts or segments are more or less uniform in size and are spherical or rounded in shape.
Any consituent part of a prokaryotic heteropolar filament.
A trichome part that is comprised of the terminal (basal) cell in a trichome, which has differentiated to form a heterocyte (heterocyst). Found in trichomes with apical-basal polarity that have an erect positionality.
tight filament coiling
filaments forming tight, regular spirals
A coiled filament shape where the shape of the coiling is tight, having increased coiling.
loose filament coiling
A coiled filament shape where the shape of the coiling is loose, having decreased coiling.
irregular filament coiling
trichomes irregularly screw-like or spirally coiled
A coiled filament shape where the shape of the coiling is irregular.
regular filament coiling
A coiled filament shape where the shape of the coiling is regular.
Bacterial thylakoid that is present in Cyanobacteria.
differentiated cyanobacterial filament part
A cyanobacterial filament part (e.g. apical cell, meristematic zone) which is differentiated.
trichome_differentiation
Prokaryotic cell having a distinctive shape.
Wikipedia:Bacillus_(shape) The word bacillus (plural bacilli) may be used to describe any rod-shaped bacterium, and such bacilli are found in many different taxonomic groups of bacteria. However, the name Bacillus, capitalized and italicized, refers to a specific genus of bacteria. The name Bacilli, capitalized but not italicized, can also refer to a more specific taxonomic class of bacteria that includes two orders, one of which contains the genus Bacillus. Bacilli are usually solitary, but can combine to form diplobacilli, streptobacilli, and palisades.
A prokaryotic cell, where one axis (the long axis, the length) of the cell is longer than the other axis (the short axis, the width) of the cell.
Wikipedia:Coccus Coccus (plural cocci or coccuses) can be used to describe any bacterium that has a spherical shape. It is one of the three distinct types of bacteria shapes, the other two being bacillus (rod-shaped) and spirillum (spiral-shaped) cells. Coccus is an English loanword of a Neolatin noun, which in turn stems from the Greek masculine noun kokkos (κόκκος) meaning "berry".
A prokaryotic cell that is spherical with no apparent long axis.
Wikipedia:Cylinder_(geometry) A cylinder (from Greek κύλινδρος – kulindros, "roller, tumbler") is one of the most basic curvilinear geometric shapes, the surface formed by the points at a fixed distance from a given line segment, the axis of the cylinder. The solid enclosed by this surface and by two planes perpendicular to the axis is also called a cylinder. The surface area and the volume of a cylinder have been known since deep antiquity.
A prokaryotic cell, being a bacillus where the long axis of the cell is much longer than the short axis of the cell.
A prokaryotic cell where one part of the cell has been made flat so that the shape of the cell is asymmetrical or irregular.
A prokaryotic cell where one end end of the cell are wider than the middle (pear shaped).
A prokaryotic cell where the surface of the cell is not rounded but is polygonal (has angular edges).
A prokaryotic cell where the cell has appendages or stalks that are extensions of the cell surface (including the cell wall and cell membrane).
Wikipedia:Prosthecate_bacteria Prosthecate bacteria are a non-phylogenetically related group of Gram-negative bacteria that possess appendages, termed prosthecae. These cellular appendages are neither pili nor flagella, as they are extensions of the cellular membrane and contain cytosol. One notable group of prosthecates is the genus Caulobacter.
A prokaryotic cell where the surface of the cell is polygonal and the angles of the cell meet at right angles forming a box.
Wikipedia:Haloquadratum Haloquadratum ("salt square") is a genus of the family Halobacteriaceae. The first species to be identified in this group, Haloquadratum walsbyi, is highly unusual since its cells are shaped like flat, square boxes.
A prokaryotic cell where the surface of the cell is polygonal and the angles of the cell meet at angles that are less than 90 degrees giving the cell a triangular or pyramidal appearance.
A sub-cylindrical, flattened prokaryotic cell where the shape is hemispherical (flattened on one side of the sphere).
Prokaryotic cells that are very small formed as a result of rapid cell division.
A prokaryotic cell, being a bacillus where one one end is wider and more rounded than the other end (i.e. being club-shaped, or cudgel-shaped). The cannonical morphology of Corynebacterium diphtheriae.
Wikipedia:Vibrio Vibrio is a genus of Gram-negative bacteria possessing a curved rod shape (comma shape), several species of which can cause foodborne infection, usually associated with eating undercooked seafood.
A prokaryotic cell, being a bacillus where the elongated axis of the cells is curved (but not spiral).
Seong-Joo L & Golubic S. 1998. Multi-trichomous cyanobacterial microfossils from the Mesoproterozoic Gaoyuzhuang Formation, China: Paleoecological and taxonomic implications. Lethaia 31:169-184. Multi-trichomous filamentous cyanobacteria of the genera Schizothrix and Microcoleus are the most commonly reported constructive elements in modern microbial mat ecosystems. .... A few specimens within the studied assemblage contain two to several parallel rows of evenly spaced internal bodies within a tube (Fig. 5C, D0, corresponding to a multi-trichomous arrangement.
Castenholz, 2001, in Bergey's Manual of Systematic Bacteriology, v. 1, 2nd ed., pg. 473.
Cyanobacterial filament with one trichome in the sheath, where the trichome is greater than two cells in width. Arises as a result of cell division occuring in two planes,one perpendicular to the axis of the trichome and one parallel to the axis of the trichome.
Cyanobacterial filament with one trichome in the sheath, where the trichome is one cell in width. Arises as a result of cell division occuring in only one plane.
sheaths envelop always only one trichome
Wiktionary:uniseriate Having one line or series; uniserial, unbranched
Prokaryotic cell having a distinctive size (width).
A distinctively-sized prokaryotic cell, where the size is small enough to pass through a filter with a defined pore size.
From: Wikipedia: Nanophytoplankton Nanophytoplankton are particularly small phytoplankton with sizes between 2 ­and 20 µm. They are the autotrophic part of nanoplankton. Like other phytoplankton, nanophytoplankton are microscopic organisms that obtain energy through the process of photosynthesis and must therefore live in the upper sunlit layer of ocean or other bodies of water. These microscopic free-floating organisms, including algae, protists, and cyanobacteria, fix large amounts of carbon which would otherwise be released as carbon dioxide.
Photosynthetic prokaryotes, or single-celled eukaryotes, that have a cell size between 2 and 20 micrometers in diameter.
From: Wikipedia: Picoplankton Picoplankton is the fraction of plankton composed by cells between 0.2 and 2 μm that can be either : Some species can also be mixotrophic. Picoplankton are responsible for the most primary productivity in oligotrophic gyres, and are distinguished from nanoplankton and microplankton. Because they are small, they have a greater surface to volume ratio, enabling them to obtain the scarce nutrients in these ecosystems. Note that the SI prefix pico- is used quite loosely here, as nanoplankton and microplankton are only 10 and 100 times larger, respectively.
Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter.
Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter and that are not photosynthetic, but rather use organic carbon as a source of energy.
From: Wikipedia: Heterotrophic_picoplankton Heterotrophic picoplankton is the fraction of plankton composed by cells between 0.2 and 2 μm that do not perform photosynthesis.
From: Wikipedia: Photosynthetic_picoplankton Photosynthetic picoplankton is the fraction of the phytoplankton performing photosynthesis composed by cells between 0.2 and 2 µm (picoplankton). It is especially important in the central oligotrophic regions of the world oceans that have very low concentration of nutrients.
Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter and that are photosynthetic (use light as a source of energy).
Prokaryotes or single-celled eukaryotes that have a cell size between 0.2 and 2 micrometers in diameter, that are photosynthetic (use light as a source of energy), and that are members of the genus Prochlorococcus and iin some members of the genus Synechococcus (both Cyanobacteria).
From: Scanlan DJ et al. 2009. Ecological genomics of marine picocyanobacteria. Microbiol Mol Biol Rev 73(2):249-299. Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus numerically dominate the picophytoplankton of the world ocean, making a key contribution to global primary production.
Filament septation, where septa separating the individual cells that make up the filament are present.
Cyanobacterial filament one or more than one trichome in the sheath, with the trichomes oriented parallel to one another, where each trichome is one cell in width. Arises as a result of cell division mostly occurring in only one plane, and occasionally occurring in a second plane.
filaments contain one or more trichomes placed together and more or less parallel within one sheath
filaments composed of one to more parallel arranged trichomes
sheaths enveloping one (rarely 2) trichomes
sheaths containing 1 to several trichomes
A heteropolar trichome that is tapered (has a larger cell diameter at one end of the filament than the other).
A prokaryotic cell that is irregular in shape, where the shapes of the cells are irregular and variable (pleomorphic). Cell shapes may be variable in size and shape, depending on the environmental conditions.
An organic-rich, mineral-salts, solid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Supplemented with starch. Used for the cultivation of heterotrophic marine microorganisms.
From: Barbeyron T, L'Haridon S, Corre E, Kloareg B & Potin P. 2001. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. IJSEM 51:985-997. The strain was assayed for amylase activity using starch at a concentration of 0.2 % (w/v) in ZoBell agar or in ZoBell Phytagel plates.
Metabolic test for tryptophanase [L-tryptophan indole-lyase (deaminating)] activity. Measures the formation of indole from tryptophan using the enzyme Tryptophanase. Indole is detected by the addition of Kovac's reagent (isoamy alchohol, para-dimethylaminobenzaldehyde, and concentrated HCl). Indole forms a complex with para-dimethylaminobenzaldehyde, resulting a red color. A negative result is yellow. In some API tests, James' reagent (a proprietary mixture of HCl, water, and 2-methoxy-4-dimethylamino benzaldehyde) is used instead of Kovac's reagent, as it has a longer shelf life. Tryptophanase catalyzes the following reaction: Tryptophan + H2O <=> indole + pyruvate + ammonia
prokaryotic cell wall lysis susceptibility
Prokaryotic cell wall quality that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (detergent, water, hypotonic solution).
susceptible to lysis by sodium dodecyl sulfate
lyse completely in sodium dodecyl sulfate
Susceptibility to lysis by detergent where the cell shows increased susceptibility toward lysis by detergent.
sensistive to lysis by sodium dodecyl sulfate
susceptibile to lysis by hypotonic solution
Susceptibility to lysis by hypotonic solution where the cell shows increased susceptibility toward lysis by hypotonic solution.
susceptible to lysis by hypotonic solution
lyse after transfer into demineralized water
Susceptibility to lysis by water where the cell shows increased susceptibility toward lysis by water.
The molar percentage of guanosine and cytidine nucleobases ([molG+molC]/[molG+molC+molA+molT)) in the DNA of a prokaryotic chromosome and/or plasmid.
The process in which nitrite is reduced to more reduced nitrogen compounds, including nitrite, nitric oxide, nitrous oxide, and dinitrogen.
Cell gliding, characterized by bacterial movement that does not involve use of a flagellum. Gliding is typically observed when a particular genus or species of microorganism encounters a surface.
The process in which nitrite is reduced to more reduced nitrogen compounds, including nitric oxide, nitrous oxide, and dinitrogen.
anaerobic respiration, using tetrathionate as electron acceptor
The process of anaerobic respiration, through the reduction of tetrathionate to thiosulfate, elemental sulfur, or hydrogen sulfide.
A gravity-driven water filter made by the British Berkefeld® company, with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter.
Pathogen role of a pathogenic prokaryotic cell.
A gravity-driven water filter made by the British Berkefeld® company, with a narrow pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms.
Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store carbon.
microbiological reactivity to lymphatic materials assay
An assay to determine if a particular microbial taxon or isolate has reactivity towards blood, blood plasma, or blood cells (including clotting).
Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store nitrogen.
From: McClung-Toabe Agar (Atlas, The Handbook of Microbiological Media for the Examination of Food, 2nd ed., pgs 221-222) Egg Yolk Emulsion, 50%: Composition per 100.0 mL: Chicken egg yolks 11 Whole chicken egg 1 NaCl (0.9% solution) 50.0 mL Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Measure 50.0 mL of egg yolk emulsion and add to 50.0 mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45-50˚C.
An emulsion of chicken egg and chicken egg yolks, created by heating an homogenized mixture of egg and egg yolks with sodium chloride.
A microbiological assay for binding to blood cells, where the cell of a microorganism adheres to red blood cells.
http://medical-dictionary.thefreedictionary.com/hemadsorption\ hemadsorption /he·mad·sorp·tion/ (hem″ad-sorp´shun) the adherence of red cells to other cells, particles, or surfaces.hemadsor´bent
A microbiological assay of lysis of blood cells, where the assay is for cytolysis of red blood cells. Hemolysis assays are carried out using some formulation of blood agar, and the assay involves determination of whether lysis has occured and the nature of the lysis.
Wikipedia:hemolysis_(microbiology) Alpha-haemolysis occurs when an organism secretes alpha-haemolysin, a protein which forms a pore in the membranes of red blood cells. This leads to the formation of colonies on blood agar that have a dark greenish appearance. The green color is caused by peroxidation of hemoglobin (presence of biliverdin).
A hemolysis assay for the process of alpha-hemolysis, where a protein secreted by a microorganism results in the formation of a pore in the membranes of erythrocytes (red blood cells) and thus causing the cells to lyse. Alpha hemolysis partially breaks down the red blood cells leaving a greenish color due to the presence of biliverdin, a by-product of hemoglobin.
A hemolysis assay for the process of beta-hemolysis, where a protein (beta-haemolysin) secreted by a microorganism results in the hyrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse. Beta-hemolysis breaks down the red blood cells and hemoglobin completely, leaving a clear zone in the agar around the growing cells or colonies.
Wikipedia:hemolysis_(microbiology) Beta hemolysis (β-hemolysis), is caused by the secretion of beta-hemolysin by a pathogenic bacterium. Beta-hemolysin is a protein which catalyzes the hydrolysis of sphingomyelin, releasing phosphoryl choline and resulting in lysis fo the red blood cell. The result are colonies on blood agar that are surrounded by a yellow, transparent zone.
A hemolysis assay for the process of gamma-hemolysis, where a protein (beta-haemolysin) secreted by a microorganism results in the hydrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse. Gamma-hemolysis does not break dow the red blood cells, and thus no clearing is seen.
Wikipedia:hemolysis_(microbiology) Gamma hemolysis, caused by the secretion of gamma-hemolysin, results in the lysis of leucocytes (white blood cells). Red blood cells are unaffected, and therefore do not show hemolytic activity on blood agar plates.
Wikipedia:hemagglutination Hemagglutination, or haemagglutination, is a specific form of agglutination that involves red blood cells (RBCs). It has two common uses in the laboratory: blood typing and the quantification of virus dilutions.
A microbiological assay for binding to blood cells, where a microorganism causes red blood cells to clump (agglutinate).
Heterotypic cell-cell adhesion where a prokaryotic cell is able to adhere to another cell.
From: Staley JT. 1981. The genera Prosthecomicrobium and Ancalomicrobium. In: Starr MP, Stolp H, Truper HG, Balows A,Schlegel HG, (eds). The prokaryotes. Berlin: Springer-Verlag. Pg 68. Vitamin Solution B12 0.1 mg Biotin 2 mg Calcium pantothenate 5 mg Folic acid 2 mg nicotinamide 5 mg pyridoxine HCl 10 mg riboflavin 5 mg thiamine HCL 5 mg Add distilled water up to 1 L and store in 4˚C in a dark container.
A solution of B vitamins including cobalamin (B12), biotin (B7), pantothenic acid (B5), folic acid (B9), nicotinamide (B3), pyridoxine (B6), riboflavin (B2), and thiamine (B1). Used to support the growth of microorganisms.
A solution of trace elements (or trace metals), sometimes also having an organic chelator of metal ions (such as EDTA or nitrilotriacetic acid). Used to support the growth of microorganisms.
A trace elements solution containing disodium EDTA, zinc sulfate, ferrous sulfate, manganese sulfate, copper sulfate, cobalt nitrate, and sodium tetraborate.
From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=149 Metals ''44'': EDTA·2Na 250.0 mg ZnSO4·7H2O 1095.0 mg FeSO4·7H2O 500.0 mg MnSO4·xH2O 154.0 mg CuSO4·5H2O 39.2 mg Co(NO3)2·6H2O 24.8 mg Na2B4O7·10H2O 17.7 mg Distilled water 1.0 L
A water extract of soil. Soil extract is often prepared by sieving air dried soil through a coarse sieve and autoclaving distilled water. Used to support the growth of microorganisms.
Chopped and ground meat (muscle tissue) containing a low fat content from beef (Bos taurus). Used to culture microorganisms.
A polymeric organic compound or mix of organic compounds which are liquid under autoclave conditions but solidify under the growth conditions used to cultivate a microorganism. Used to make solid microbiological culture media.
Defined organic solution comprised of a mixture of vitamins (used as enzyme co-factors) added in small amounts to microbiological culture medium. Used to support the growth of microorganisms.
Microbiological medium ingredient, derived from chicken egg, where the chicken egg has been homogenized (mixed).
An enzymatic hydrolysis of protein derived from fish (of unspecified origin), used for the cultivation of microorganisms.
microbiological medium ingredient, derived from enzymatic hydrolysis of mammalian protein
Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of animal protein (mammal meat, muscle tissue).
A pancreatic digest of heart tissue derived from animal (mammalian) tissue, used for the culturing of microorganisms.
microbiological medium ingredient, derived from enzymatic hydrolysis of milk protein
Protein hydrolysates, also called peptones, are the result of the enzymatic hydrolysis of milk proteins (such as casein or lactalbumin).
A pancreatic digest of gelatin, extracted from collagen derived from bone, cartilage, and connective tissue (of unspecified origin).
microbiological culture medium, promotes growth of autotrophs
A microbiological culture medium that lacks organic carbon compounds and therefore promotes the growth of autotrophic microorganisms.
microbiological culture medium, promotes growth of heterotrophs
A microbiological culture medium that contains organic carbon compounds and therefore promotes the growth of heterotrophic microorganisms.
A mineral-salts, solid microbiological culture medium with yeast extract, ammonium phosphate, and carboxymethyl cellulose. Used for the cultivation of microorganisms capable of metabolizing cellulose.
From: BugwoodWiki:CMCA (Carboxymethylcellulose agar) Medium contains: Part 1: Distilled water, 1 L NH4H2PO4, 1 g KCl, 0.2 g MgSO4*7H2O, 1 g Yeast extract, 1 g Part 2: Carboxymethyl cellulose, 26 g Agar, 3g Mix ingredients from Part 1 and add ingredients from Part 2 while stirring Autoclave 20 minutes.
A solid microbiological culture medium that has been solidified by agar and poured into a flat surface in a Petri dish.
hypersaline microbiological culture medium
A microbiological culture medium that has a salinity of greater than 5% salt.
A microbiological culture medium that has a salinity of 3-5 % salts.
A microbiological culture medium that has a salinity of greater than 0.05 % salts and less than 3% salts.
A prokaryotic quality relating the optimal physiological preference (pressure, salinity, temperature) of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division.
Wikipedia:piezophile Barophilic microorganisms grow at high pressures. They are typically isolated from deep subsurface or deep sea habitats.
Pressure optimum quality, where growth rates at elevated pressures are higher than growth rates at atmospheric pressure. Generally a characteristic of prokaryotic microorganisms that grow in the deep oceans.
Magnesium (II) ions are required to be present in order for growth of a particular microorganism to occur.
An assay used to identify or characterize a microorganism. Is used to determine the biological process, quality, cellular component, molecular function, or a chemical entity that is associated with the particular microorganism.
An enzymatic assay which tests for the presence of urease in a microorganism. Urease hydrolyzes urea to produce CO2 and ammonia, which increases the pH of the media: (NH2)2CO + H2O -> CO2 + 2 NH3 The urease test uses phenol red as a pH indicator. An increase in alkalinity results in a color from yellow to pink/red. A positive result is pink/orange/red; a negative test result is yellow. The test employs either Christensen's Urea Agar or Stuart's Urea Broth.
Wikipedia:piezophlie Barotolerant microorganisms are able to grow or survive at high pressures, but can also grow and survive at atmospheric pressures.
Pressure optimum quality, where some growth occurs at elevated pressures, but growth is fastest at atmospheric pressures.
Barophilic, and can only grow at elevated pressures. These organisms require elevated pressure to grow and survive.
The metabolic quality of a microorganism with respect to its relationship to oxygen.
Wikipedia:fermentation Fermentation is a metabolic process of microorganisms which converts a carbohydrate carbon and energy substrate into acids, gases, and/or alcohols.
A prokaryotic metabolic process where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system.
A prokaryotic metabolic process which involves the oxidation and reduction of chemical compounds and involves an electron transport chain.
Wikipedia:aerobic organism An aerobic organism or aerobe is an organism that can survive and grow in an oxygenated environment. Facultative anaerobes grow and survive in an oxygenated environment and so do aerotolerant anaerobes.
A prokaryotic metabolic quality where the organism grows in the presence of oxygen.
Wikipedia:anaerobic_organism An anaerobic organism or anaerobe is any organism that does not require oxygen for growth. It may react negatively or even die if oxygen is present, which means that it can perform its bodily functions better in the absence of oxygen. An anaerobic organism may be unicellular or multicellular (like metazoa or more complex organisms like deep sea worms). A few parasites like Trichinella spiralis (pork worm) respire anaerobically in nurse cells (infected cell playing host to the juvenile parasite). Some largely unicellular anaerobic microbes are protozoans, but most of the anaerobic microbes are bacteria or Archaea. For practical purposes there are three categories: obligate anaerobes, which are harmed by the presence of oxygen aerotolerant organisms, which cannot use oxygen for growth, but tolerate the presence of it facultative anaerobes, which can grow without oxygen but can utilize oxygen if it is present
A prokaryotic metabolic quality where the organism grows in the absence of oxygen.
Wikipedia:Chemotroph Chemotrophs are organisms that obtain energy by the oxidation of electron donors in their environments. These molecules can be organic (chemoorganotrophs) or inorganic (chemolithotrophs). The chemotroph designation is in contrast to phototrophs, which utilize solar energy. Chemotrophs can be either autotrophic or heterotrophic.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source.
Wikipedia:Mixotroph A mixotroph is an organism that can use a mix of different sources of energy and carbon. Possible alternations are between photo- and chemotrophy, between litho- and organotrophy, between auto- and heterotrophy or a combination of it. Mixotrophs can be either eukaryotic or prokaryotic. They can take advantage of different environmental conditions. If a trophic mode is obligate, then it is always necessary for sustaining growth and maintenance; if facultative, it can be used as a supplemental source. Some organisms have incomplete Calvin cycles, so they are incapable of fixing carbon dioxide and must use organic carbon sources.
A prokaryotic respiratory metabolic process where phototrophy and heterotrophy co-occur at the same instance in time.
A prokaryotic respiratory metabolic process where light is the energy source.
Wikipedia:Phototroph Phototrophs are the organisms that carry out photon capture to acquire energy. They use the energy from light to carry out various cellular metabolic processes. Most of the well-recognized phototrophs are autotrophs, also known as photoautotrophs, and can fix carbon.
A prokaryotic respiratory metabolic process where light is the energy source, and the electron donor is an inorganic compound.
Wikipedia:Lithotroph Photolithotrophs obtain energy from light and therefore use inorganic electron donors only to fuel biosynthetic reactions (e.g., carbon dioxide fixation in lithoautotrophs). These bacteria are photosynthetic; photolithotrophic bacteria are found in the purple bacteria (e. g., Chromatiaceae), green bacteria (Chlorobiaceae and Chloroflexi) and Cyanobacteria. Purple and green bacteria oxidize sulfide, sulfur, sulfite, iron or hydrogen. Cyanobacteria extract reducing equivalents from water, i.e., they oxidise water to oxygen. The electrons obtained from the electron donors are not used for ATP production (as long as there is light); they are used in biosynthetic reactions. Some photolithotrophs shift over to chemolithotrophic metabolism in the dark.
Wikipedia:Microbial metabolism Photolithoautotrophs obtain energy from light and carbon from the fixation of carbon dioxide, using reducing equivalents from inorganic compounds. Examples: Cyanobacteria (water (H2O) as reducing equivalent donor), Chlorobiaceae, Chromatiaceae (hydrogen sulfide (H2S) as reducing equivalent donor), Chloroflexus (hydrogen (H2) as reducing equivalent donor).
A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide
A prokaryotic metabolic process that involves fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms.
Wikipedia: Photofermentation Photofermentation is the fermentative conversion of organic substrate to biohydrogen manifested by a diverse group of photosynthetic bacteria by a series of biochemical reactions involving three steps similar to anaerobic conversion. Photofermentation differs from dark fermentation because it only proceeds in the presence of light. For example photo-fermentation with Rhodobacter sphaeroides SH2C (or many other purple non-sulfur bacteria[1]) can be employed to convert small molecular fatty acids into hydrogen[2] and other products.
Wikipedia:anaerobic_organism An anaerobic organism or anaerobe is any organism that does not require oxygen for growth. For practical purposes there are three categories: obligate anaerobes, which are harmed by the presence of oxygen aerotolerant organisms, which cannot use oxygen for growth, but tolerate the presence of it facultative anaerobes, which can grow without oxygen but can utilize oxygen if it is present
A prokaryotic metabolic quality where the organism grows in the absence of oxygen. Can grow or live in the presence of oxygen.
Wikipedia:facultative_anaerobic_organism A facultative anaerobic organism is an organism, usually a bacterium, that makes ATP by aerobic respiration if oxygen is present but is also capable of switching to fermentation. In contrast, obligate anaerobes die in the presence of oxygen.
A prokaryotic metabolic quality where the organism grows in the presence of oxygen or in the absence of oxygen.
Wikipedia:obligate_anaerobe Obligate anaerobes are microorganisms that live and grow in the absence of molecular oxygen; some of these are killed by oxygen.
A prokaryotic metabolic quality where the organism grows in the absence of oxygen. Cannot grow or live in the presence of oxygen.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound.
Wikipedia:Lithotroph A chemolithotroph (named after the process of chemolithotropy) is a type of extremophile that is able to use inorganic reduced compounds as a source of energy. This process is accomplished through oxidation and ATP synthesis. Most chemolithotrophs are able to fix carbon dioxide (CO2) through the Calvin Cycle, a metabolic pathway in which carbon enters as CO2 and leaves as glucose. This group of organisms includes sulfur oxidizers, nitrifying bacteria, iron oxidizers, and hydrogen oxidizers. The term chemolithotropy refers to a cell’s acquisition of energy from the oxidation of inorganic compounds, also known as electron donors, in the absence of light. Chemolithotropy is a form of metabolism, and the extreme conditions under which it can take place leads to its classification as a branch of the extremophiles. This process only occurs in prokaryotes, and was first characterized by the Russian microbiologist Sergej Winogradsky.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound.
Wikipedia:Primary nutritional groups Chemoorganotrophs are organisms which oxidize the chemical bonds in organic compounds as their energy source. Chemoorganotrophs also attain the carbon molecules that they need for cellular function from these organic compounds. The organic compounds that they oxidize include sugars (i.e. glucose), fats and proteins).[2]
A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryotic respiratory metabolic process where light is the energy source, and the electron donor is an organic compound.
Wikipedia:Photoheterotroph Photoheterotrophs are heterotrophic organisms that use light for energy, but cannot use carbon dioxide as their sole carbon source. Consequently, they use organic compounds from the environment to satisfy their carbon requirements; these compounds include carbohydrates, fatty acids, and alcohols. Examples of photoheterotrophic organisms include purple non-sulfur bacteria, green non-sulfur bacteria, and heliobacteria.
A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide.
Wikipedia:Microbial metabolism Photoorganoheterotrophs obtain energy from light, carbon and reducing equivalents for biosynthetic reactions from organic compounds. Some species are strictly heterotrophic, many others can also fix carbon dioxide and are mixotrophic. Examples: Rhodobacter, Rhodopseudomonas, Rhodospirillum, Rhodomicrobium, Rhodocyclus, Heliobacterium, Chloroflexus (alternatively to photolithoautotrophy with hydrogen).
A prokaryotic respiratory metabolic process where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
Wikipedia:Microbial metabolism Chemolithoautotrophs obtain energy from the oxidation of inorganic compounds and carbon from the fixation of carbon dioxide. Examples: Nitrifying bacteria, Sulfur-oxidizing bacteria, Iron-oxidizing bacteria, Knallgas-bacteria.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide.
Wikipedia:Microbial metabolism Chemolithoheterotrophs obtain energy from the oxidation of inorganic compounds, but cannot fix carbon dioxide (CO2). Examples: some Thiobacillus, some Beggiatoa, some Nitrobacter spp., Wolinella (with H2 as reducing equivalent donor), some Knallgas-bacteria, some sulfate-reducing bacteria.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide.
A prokaryotic respiratory metabolic process where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
Wikipedia:Primary nutritional groups A chemoorganoheterotrophic organism is one that requires organic substrates to get its carbon for growth and development, and that produces its energy from oxido-reduction of an organic compound. This group of organisms may be further subdivided according to what kind of organic substrate and compound they use. Decomposers are examples of Chemoorganoheterotrophs which obtain carbon and electron reactions from dead organic matter. Herbivores and carnivores are examples of organisms that obtain carbon and electron reactions from living organic matter.
A prokaryotic metabolic quality where the organism grows in the presence of oxygen, where the oxygen concentration is less than atmospheric levels.
Wikipedia:microaerophile A microaerophile is a microorganism that requires oxygen to survive, but requires environments containing lower levels of oxygen than are present in the atmosphere (~20% concentration). Many microphiles are also capnophiles, as they require an elevated concentration of carbon dioxide. In the laboratory they can be easily cultivated in a candle jar. A candle jar is a container into which a lit candle is introduced before sealing the container's airtight lid. The candle's flame burns until extinguished by oxygen deprivation, which creates a carbon dioxide-rich, oxygen-poor atmosphere in the jar.
A prokaryotic metabolic quality where the organism grows in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen).
Wikipedia:obligate_aerobe An obligate aerobe is an aerobic organism that requires oxygen to grow. Through cellular respiration, these organisms use oxygen to oxidize substances, like sugars or fats, in order to obtain energy. During respiration, they use oxygen as the terminal electron acceptor. They have the advantage of yielding more energy than obligate anaerobes, but face high levels of oxidative stress.
microbiological medium ingredient, derived from extracts of plants
Undefined mixtures of complex organic compounds deriving from the aqueous extraction (an extraction using water, such as hot water or steam) of macroscopic plants. Used in the cultivation of microorganisms.
Organic compounds or mixtures of organic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is known, it is referred to as "defined".
Undefined organic chemical mixture, comprised of ruminal fluid derived from the rumen of a cow, which has been clarified (through the processes of heating/autoclaving and then centrifugation). Used to cultivate microorganisms.
From Wikipedia:Sludge: Sludge is a semi-solid slurry and can be produced as sewage sludge from wastewater treatment processes or as a settled suspension obtained from conventional drinking water treatment and numerous other industrial processes.
Fluid derived from the liquid component of municipal sludge, used for the cultivation of microorganisms.
The water-soluble portion of autolyzed yeast (Saccharomyces cereviseiae), used to support the growth of microorganisms. Yeastolate is a branch name for a yeast extract sold by BD.
From BD Bionutrients Technical Manual (3rd edition revised): TC Yeastolate products are animal-free and water-soluble portions of autolyzed yeast or Saccharomyces cerevisiae. TC Yeastolate is a mixture of peptides, amino acids, carbohydrates, simple and complex as well as vitamins. Ash content is 11.7% NaCl content is 0.6%
A prokaryotic cellularity where cells are physically attached, or connected, to one another after cell division occurs. Septation may or may not occur. The connections between the cells may be loose and transient, or may be long-lasting and permanent.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Chocolatized Defibrinated Blood is defibrinated blood that has been heated to release additional growth factors that would otherwise remain unavailable within intact red blood cells. Heat stable hemin (X-factor) and heat labile nicotinamide adenine dinucleotide (V-factor) are the growth factors readily available in chocolatized media.
Blood medium ingredient comprised of defibrinated blood that has been heated. Used in the cultivation of microorganisms.
Serum medium ingredient comprised of blood serum from a mammal that has been inspissated (dehydrated, i.e. has a decreased water composition). Used to support the growth of microorganisms which need inspissated blood serum to grow.
From Wikipedia:Inspissation: Inspissation is the process of thickening by dehydration.
Prokaryotic cells which have a morphological shape and a role that is different from vegetative (undifferentiated cells).
prokaryotic differentiated cell, specialized for nitrogen fixation
Prokaryotic differentiated cell that has a role in the fixation of gaseous dinitrogen (N2) into ammonia and thus enhance survival in nitrogen-deficient environments.
prokaryotic differentiated cell, specialized for asexual reproduction
Prokaryotic differentiated cell that has a role in asexual reproduction.
prokaryotic differentiated cell, specialized for dormancy
Prokaryotic differentiated cell which contributes to enhanced dormancy and thus survival during times unsuitable for growth.
An organic-rich, marine, liquid microbiological culture medium containing yeast extract, polypeptone, casamino acids, and glycerol in an artificial sea water base. Used for the growth Erythrobacter.
Shioi Y. 1986. Growth characteristics and substrate specificity of aerobic photosynthetic bacterium, Erythrobacter sp. Och-114. Plant Cell Physiol 27:567-572. Shioi (1986) used a medium which contains in 1 liter of distilled water: 20 g NaCl, 5.0 g MgCl2x6H2O, 2.0 g Na2SO4, 0.5 g KCl, 0.5 g CaCl2x2H2O, 0.2 g NaHCO3, 0.1 g ferric citrate, 2.0 g yeast extract (Difco), 1.0 g polypeptone, 1.0 g Casamino acids, and 1.0 mL glycerol.
The pH is not in any available references online or in Bergey's Manual. However is likely slightly alkaline due to the buffering of sodium bicarbonate.
Shiba T & Imhoff JF. 2005. Genus XVIII. Roseobacter in Bergey's Manual of Systematic Bacteriology, 2nd Ed. Vol 2, Part C, p. 213.
From: http://cshprotocols.cshlp.org/content/2008/12/pdb.rec11558.full?text_only=true SM Broth Composition: Reagent Quantity (for 1 L) Final concentration Glucose 10 g 56 mM KH2PO4 1.9 g 14 mM K2HPO4 0.6 g 3.4 mM NH4Cl 1 g 20 mM Proteose peptone 2 ( Difco 212120) 10 g 1% Yeast extract (Oxoid LP0021) 1 g 0.1% Dissolve in 1 L of H2O. Adjust the pH to 6.0–6.4 with KOH.
A liquid microbiological culture medium containing gelatin, magnesium sulfate, and sodium chloride. Used for the storage and dilution of Escherichia coli bacteriophage lambda.
From: https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/c0422dat.pdf CMRL-1066 With L-Glutamine, Without Sodium Bicarbonate Components g/L L-Alanine 0.025 L-Arginine 0.05787 L-Aspartic Acid 0.03 L-Cysteine•HCl• H2O 0.26 L-Cystine 0.02 L-Glutamic Acid 0.075 L-Glutamine 0.1 Glycine 0.05 L-Histidine HCl• H2O 0.02 Trans-4-Hydroxy-L-Proline 0.01 L-Isoleucine 0.02 L-Leucine 0.06 L-Lysine•HCl 0.07 L-Methionine 0.015 L-Phenylalanine 0.025 L-Proline 0.04 L-Serine 0.025 L-Threonine 0.03 L-Tryptophan 0.01 L-Tyrosine 0.04 L-Valine 0.025 L-Ascorbic Acid 0.05 PABA 0.00005 D-Biotin 0.00001 Choline Chloride 0.0005 Coenzyme A•Na 0.0025 Cocarboxylase 0.001 2'-Deoxyadenosine 0.01 2'-Deoxyguanosine 0.01 2'-Deoxycytidine•HCl 0.0116 Flavin Aadenine Dinucleotide•2Na 0.000106 Folic Acid 0.00001 myo-Inositol 0.00005 5-Methyldeoxycytidine 0.0001 beta-NAD 0.007 beta-NADP•Na 0.001 Niacinamide 0.000025 Nicotinic Acid 0.000025 D-Pantothenic Acid [hemicalcium] 0.00001 Pyridoxal•HCl 0.000025 Pyridoxine•HCl 0.000025 Riboflavin 0.00001 Thiamine•HCl 0.00001 Thymidine 0.01 Uridine-5-Triphosphate•Na 0.001 Calcium Chloride [Anhydrous] 0.2 Magnesium Sulfate [Anhydrous] 0.09769 Potassium Chloride 0.4 Sodium Acetate [Anhydrous] 0.05 Sodium Chloride 6.8 Sodium Phosphate Monobasic [Anhydrous] 0.122 D-Glucose 1.0 Phenol Red•Na 0.02124 Glutathione 0.01 D-Glucuronic Acid•Na 0.00388 Cholesterol 0.0002 Tween 80 0.005 Sodium bicarbonate is added during the preparation step and the pH is adjusted to 7.0 ± 0.3.
A defined liquid microbiological culture medium containing glucose, a pH indicator, a mixture of amino acids, vitamins, co-factors, and organic micronutrients. Used for tissue cell culture.
From: Skim Milk Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Skim Milk Medium may be used for the cultivation and differentiation of microorganisms based on the coagulation and proteolysis of casein. Principles of the Procedure Skim Milk is a source of lactose and casein and other nutrients required for the growth of lactobacilli.4 Clostridial species can be differentiated based on their ability to enzymatically degrade proteins to peptones (peptonization) or coagulate milk.5 It may be used to detect the stormy fermentation produced by Clostridium perfringens. Formula Difco(TM) Skim Milk Approximate Formula* Per Liter Skim Milk Powder 100.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.3 ± 0.2
An organic-rich, liquid microbiological culture medium containing skim milk powder. Used to cultivate microorganisms that can metabolize casein.
An organic-ric, solid microbiological culture medium containing skim milk powder. Used to cultivate microorganisms that can metabolize casein.
An organic-rich, solid microbiological culture medium, containing peptones, starch, and sheep blood. Used for the growth of heterotrophic microorganisms that require blood.
Heart infusion agar supplemented with sheep blood
From: Columbia Agar with 5% Sheep Blood (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Columbia Agar Base, without or with the addition of 5% (or 10%) sheep blood, is a highly nutritious, general-purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. Difco™ Columbia Blood Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Proteose Peptone No. 3................................................ 5.0 g Yeast Extract................................................................ 5.0 g Beef Heart, Infusion from 500 g................................... 3.0 g Corn Starch.................................................................. 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g Directions for Preparation from Dehydrated Product 1. Suspend the powder in 1 L of purified water: Difco™ Columbia Blood Agar Base – 44 g; BBL™ Columbia Agar Base – 42.5 g; Difco™ Columbia Blood Agar Base EH – 39 g. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. For preparation of blood agar, cool the base to 45-50°C and add 5% sterile, defibrinated blood. Mix well. 5. Test samples of the finished product for performance using stable, typical control cultures.
An organic-rich, solid microbiological culture medium, containing peptones and starch. Used for the growth of heterotrophic microorganisms.
From: Columbia Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Columbia Agar Base, without or with the addition of 5% (or 10%) sheep blood, is a highly nutritious, general-purpose medium for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical materials. Principles of the Procedure Columbia Agar Base supplemented with sheep, rabbit or horse blood derives its superior growth-supporting properties from the combination of peptones prepared from pancreatic digest of casein, meat peptic digest and heart pancreatic digest. Yeast extract and corn starch are also included in the formulation and serve as energy sources with yeast extract being a supplier of the B-complex vitamins. Sodium chloride maintains osmotic balance in the medium. It should be noted that Columbia Sheep Blood Agar has a relatively high carbohydrate content and, therefore, beta-hemolytic streptococci may produce a greenish hemolytic reaction that may be mistaken for alpha hemolysis. Fildes enrichment is prepared by the action of the enzyme pepsin on defibrinated sheep blood. Bacitracin is a polypeptide antibiotic that is active mainly against gram-positive bacteria. BBL™ Columbia Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Meat Peptic Digest....................................................... 5.0 g Yeast Extract................................................................ 5.0 g Heart Pancreatic Digest................................................ 3.0 g Corn Starch.................................................................. 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g pH 7.3 ± 0.2
Humphry DR, George A, Black GW & Cummings SP. 2001. Flavobacteirum frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminariolytic bacterium from Antarctica. IJSEM 51:1235-1243.
From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium246.pdf DSMZ Medium 246 246. SEA WATER AGAR Beef extract 10.0 g Peptone 10.0 g Agar 20.0 g Tap water 250.0 ml Sea water* 750.0 ml Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, marine, solid microbiological culture medium containing beef extract and peptone, in a diluted artificial sea water base. Used for the growth of marine Flavobacterium.
From: Yi H, Cho J-C, & Chun J. 2012. Flavivirga jejuensis gen. nov., sp. nov., and Flavivirga amylovorans sp. nov., new members of the family Flavobacteriaceae isolated from seawater, and emended descriptions of the genera Psychroserpens and Lacinutrix. IJSEM 62:1061-1068. Growth in synthetic ZoBell broth was tested at 5-50˚C (at 5˚C intervals) and in the presence of 0, 0.5, 1, 2, 3, 4, 5, 7, 10, 12, 15 and 20% (w/v) NaCl or sea salts (Sigma).
An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Made without sodium chloride. Used to test the salinity preference of marine heterotrophic microorganisms.
An organic-rich, solid microbiological culture medium containing peptones, yeast extract, sodium chloride, glucose, sodium sulfite, hemin, vitamin K, and laked rabbit blood. Used for the growth of heterotrophic microorganisms that require blood.
From: Atlas RM, Handbook of Microbiological Media, 3rd edition, pg. 1472 Composition per liter: Agar 15.0 g Pancreatic digest of casein 10.0 g Peptic digest of animal tissue 10.0 g NaCl 5.0 g Yeast extract 2.0 g Glucose 1.0 g NaHSO3 0.1 g Rabbit blood, laked 50.0 mL Hemin solution 1.0 mL Vitamin K1 solution 1.0 mL pH 7.0 +/- 0.2 at 25˚C Hemin solution: Composition per 100.0 mL: Hemin 0.5 g NaOH (1N solution) 10.0 mL Preparation of Hemin Solution: Add hemin to 10.0 mL of NaOH solution. Mix thoroughly. Vitamin K1 solution: Composition per 20.0 mL: Vitamin K1 (phytomenadione) 0.2 g Ethanol (95% solution) 20.0 mL Preparation of Vitamin K1 Solution: Add vitamin K1 to 20.0 mL of ethanol. Mix thoroughly. Preparation of Medium: Add components, except vitamin K1 solution and laked rabbit blood, to distilled/deionized water and bring volume to 849.0 mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 45-55˚C. Aseptically add 1.0 mL of sterile vitamin K1 solution and 50.0 mL of sterile laked rabbit blood. Laked blood is prepared by freezing whole blood overnight and thawing to room temperature. Mix thoroughly. Pour into steril Petri dishes or distribute into sterile tubes. Use: For the cultivation and enhancement of pigment production of a variety of anaerobic bacteria.
An organic-rich, liquid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Used for the growth of heterotrophic bacteria from potable (fresh) water.
From: Wikipedia: R2a agar R2A agar (Reasoner´s 2A agar) is a culture medium developed to study bacteria which normally inhabit potable water. These bacteria tend to be slow-growing species and would quickly be suppressed by faster-growing species on a richer culture medium. Since its development in 1979, it has been found to allow the culturing of many other bacteria that will not readily grow on fuller, complex organic media. Typical Composition (g/l) Proteose peptone, 0.5 Casamino acids, 0.5 Yeast extract, 0.5 Dextrose, 0.5 Soluble starch, 0.5 Dipotassium phosphate, 0.3 Magnesium sulfate 7H2O, 0.05 Sodium pyruvate, 0.3 Agar, 15 Final pH 7 ± 0.2
From: Reasoner DJ & Geldreich EE. 1985. A new medium for the enumeration and subculture of bacteria from potable water. AEM 49(1):1-7. Composiotn of R3A Experimental Media (From Table 1): Yeast extract, 1.0 g/L Difco Proteose Peptone No. 3, 1.0 g/L Casamino Acids, 1.0 g/L Glucose, 1.0 g/L Soluble starch, 1.0 g/L Sodium pyruvate, 0.5 g/L K2HPO4, 0.6 g/L MgSO4x7H2O, 0.1 g/L Agar, 15.0 g/L Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C and 15 lb/in2.
An organic-rich, liquid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Concentration of organic and inorganic constituents are twice that in R2A medium. Used for the growth of heterotrophic bacteria from potable (fresh) water.
An organic-rich, solid microbiological culture medium containing yeast extract, proteose peptone, casamino acids, glucose, starch, and pyruvate. Used for the growth of heterotrophic bacteria from potable (fresh) water.
An organic-rich, liquid microbiological culture medium containing yeast extract, peptone, and dextrose (D-glucose). Used for the cultivation of yeasts.
From: Yeast Extract-Peptone-Dextrose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use YPD Agar and YPD Broth are used for maintaining and propagating yeasts in molecular microbiology procedures. Principles of the Procedure YPD Agar and YPD Broth contain peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Dextrose is the carbohydrate source. YPD Agar contains agar as the solidifying agent. Formulae Difco™ YPD Agar Approximate Formula* Per Liter Yeast Extract.............................................................. 10.0 g Peptone..................................................................... 20.0 g Dextrose.................................................................... 20.0 g Agar.......................................................................... 15.0 g Difco™ YPD Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 6.5 ± 0.2
An organic-rich, solid microbiological culture medium containing yeast extract, peptone, and dextrose (D-glucose). Used for the cultivation of yeasts.
An organic-rich, liquid microbiological culture medium containing yeast extract, peptone, and glucose. Supplemented with Tween 80 (0.02%, aka polysorbate 80). Used for the cultivation of Bacteroides spp.
Peptone-yeast extract-tween basal medium
From: Cato EP, Holdeman LV, & Moore WEC. 1979. Proposal of neotype strains for seven non-saccharolytic Bacteroides species. IJSB 29(4):427-434. Growth was stimulated by the addition of ca. 0.02% Tween 80 to the media.
From: Kampfer P, Busse H-J, Longaric I, Rossello-Mora R, Galatis H, & Lodders N. 2012. Pseudarcicella hirudinis gen. nov., sp. nov., isolated from the skin of the medical leech Hirudo medicinalis. IJSEM 62:2247-2251. 0.3% (w/v) peptone from casein 0.3% (w/v) yeast extract pH 7.2 at 28˚C
An organic-rich, liquid microbiological culture medium containing casitone and yeast extract. Used for the growth of Pseudarcicella sp.
From: Peptone Water (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Peptone Water is used for cultivating nonfastidious organisms, for studying carbohydrate fermentation patterns and for performing the indole test. Principles of the Procedure Peptone Water contains peptone as a source of carbon, nitrogen, vitamins and minerals. Sodium chloride maintains the osmotic balance of the medium. Formula Difco™ Peptone Water Approximate Formula* Per Liter Peptone..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2
An organic-rich, liquid microbiological culture medium containing peptone and sodium chloride.
An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, and dextrose. Used for the enumeration of microorganisms that grow in dairy products.
From: Plate Count Agar/Standard Methods Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Plate Count Agar and Standard Methods Agar (Plate Count Agar; Tryptone Glucose Yeast Agar) are used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance. Principles of the Procedure Enzymatic digest of casein provides the amino acids and other complex nitrogenous substances necessary to support bacterial growth. Yeast extract primarily supplies the B-complex vitamins, and dextrose is an energy source. TTC is reduced to the insoluble formazan inside the bacterial cell producing red-colored colonies. Formula Difco™ Plate Count Agar or BBL™ Standard Methods Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Yeast Extract................................................................ 2.5 g Dextrose...................................................................... 1.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, solid microbiological culture medium containing peptone, potassium sulfate, magnesium chloride, and Irgasan (aka triclosan; a broad spectrum antibiotic). Is a selective medium used for the isoaltion and growth of Pseudomonas.
From: Pseudomonas Isolation Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Pseudomonas Isolation Agar is used with added glycerol in isolating Pseudomonas and differentiating Pseudomonas aeruginosa from other pseudomonads based on pigment formation. Principles of the Procedure Peptone provides the carbon and nitrogen necessary for bacterial growth. Magnesium chloride and potassium sulfate promote production of pyocyanin. Irgasan, an antimicrobial agent, selectively inhibits gram-positive and gram-negative bacteria other than Pseudomonas spp. Agar is the solidifying agent. Glycerol serves as an energy source and also helps to promote pyocyanin production. Formula Difco™ Pseudomonas Isolation Agar Approximate Formula* Per Liter Peptone..................................................................... 20.0 g Magnesium Chloride.................................................... 1.4 g Potassium Sulfate....................................................... 10.0 g Irgasan™..................................................................... 25.0 mg Agar.......................................................................... 13.6 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, liquid microbiological culture medium containing peptone, glucose, sodium chloride, and a pH indicator (bromthymol blue). Used to test for the ability of a microorganism to metabolize specific carbohydrates, which are added to the basal medium.
From: http://www.microbelibrary.org/component/resource/laboratory-test/3151-oxidative-fermentative-test-protocol RECIPE Hugh and Leifson’s OF basal medium (6, 8) Peptone (tryptone) 2.0 g Sodium chloride 5.0 g Glucose (or other carbohydrate)a 10.0 g Bromthymol blue 0.03 g Agar 3.0 g Dipotassium phosphate 0.30 g Bring to 1 liter with distilled water. The pH should be adjusted to 7.1 prior to autoclaving (7). After the medium is autoclaved at 121°C for 15 minutes, a filter sterilized solution of 10% solution of carbohydrate (6, 8) is aseptically added to the medium to a final concentration of 1%. The sterile medium containing the carbohydrate is aliquoted aseptically into sterile test tubes and cooled unslanted as stabs (5). Some procedures call for the addition of 10 g/liter of carbohydrate to the medium prior to sterilization. The medium is then dissolved by heating to a boil on a hot plate or by steaming for 20 minutes prior to aliquoting into test tubes. The tubed medium is then steamed for 20 minutes in place of autoclaving to prevent breaking down of the carbohydrate (7). OF basal medium is commercially available in a premixed form from biological supply companies. The carbohydrate source is not included and must be added as stated above.
From: Marine Cations Supplement 1558 (From: Farmer, J. J., III & Hickman-Brenner, F. W. (2006). The genera Vibrio and Photobacterium. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 6, pp. 508–563. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer., pages 532 and 535). This medium is useful for increasing the salt content of bacteriological media to enhance the growth of marine bacteria. It contains Na+, K+, Mg++, and Ca++ at 10 times the in-use concentration. It is our formulation which was modified from the “electrolyte supplement” of Furniss et al. (1978), and it is added in the ratio of one volume of supplement to nine volumes of medium. Sodium chloride (NaCl) 150 g Potassium chloride (KCl) 3.7 g Magnesium chloride (MgCl2 · 6H2O) 51 g Calcium chloride (CaCl2 · 2H20) 7.4 g Water 912 ml Dissolve the ingredients in the order listed. All should dissolve readily, and a crystal-clear, colorless solution should result. The volume of the solution will expand to 1 liter after the salts dissolve. Dispense and autoclave at 121°C for 15 minutes. It should remain crystal clear after autoclaving, but a slight amount of fine precipitate may form and settle to the bottom of the container. To use this supplement in other media: aseptically add one volume of marine cations, supplement 1558 to nine volumes of the sterile medium, and mix thoroughly.
An organic-rich, solid microbiological culture medium containing peptone, glucose, sodium chloride, and a pH indicator (bromthymol blue). Supplemented with MCS (marine cations supplement), which includes sodium chloride, potassium chloride, magnesium chloride, and calcium chloride. Used to test for the ability of a marine microorganism to metabolize specific carbohydrates, which are added to the basal medium.
Lucena T, Pascual J, Giordano A, Gambacorta A, Garay E, Arahal DR, Macian MC, & Pujalte MJ. 2010. Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae. IJSEM 60:2871-2876. MCS = marine cations supplement
From: Nutrient Agar 1.5 % (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Nutrient Agar 1.5% is used for cultivating a variety of microorganisms and with the addition of blood or other enrichment can be used for cultivating fastidious microorganisms. Principles of the Procedure Beef extract and peptone provide the nitrogen, vitamins, amino acids and carbon sources in Nutrient Agar 1.5%. Sodium chloride maintains the osmotic balance so that red blood cells will not rupture when blood is added as supplement.1 Agar is the solidifying agent. Formula Difco™ Nutrient Agar 1.5% Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Peptone....................................................................... 5.0 g Sodium Chloride.......................................................... 8.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2
An organic-rich, solid microbiological culture medium containing beef extract, peptone and sodium chloride (8g/L). Used for the cultivation of general heterotrophic microorganisms.
A slightly-acidic, organic-rich, solid microbiological culture medium containing peptones, egg extract, yeast extract, glucose, Tween 80, and minerals salts. Used for the cultivation of Lactobacilli.
From: Wikipedia:MRS_agar Often abbreviated to MRS, this type of bacterial growth medium is so-named by its inventors: de Man, Rogosa and Sharpe. Developed in 1960, this medium was designed to favour the luxuriant growth of Lactobacilli for lab study. It contains sodium acetate, which suppresses the growth of many competing bacteria (although some other Lactobacillales, like Leuconostoc and Pediococcus, may grow). This medium has a clear brown colour.[1] MRS agar typically contains(w/v):[2] 1.0 % peptone 0.8 % egg extract 0.4 % yeast extract 2.0 % glucose 0.5 % sodium acetate trihydrate 0.1 % polysorbate 80 (also known as Tween 80) 0.2 % dipotassium hydrogen phosphate 0.2 % triammonium citrate 0.02 % magnesium sulfate heptahydrate 0.005 % manganese sulfate tetrahydrate 1.0 % agar pH adjusted to 6.2 at 25°C
A dilute organic carbon-containing, solid microbiological culture medium with glucose, yeast extract, and divalent mineral-salts. Used for the cultivation of Mucilagibacter sp.
The pH of the medium is not specified, and the medium itself is unbuffered. The optimal pH of growth for the organism in the study, however, was pH 6.0-6.5.
From: Pankratov TA, Tindall BJ, Liesack W, & Dedysh SN. 2007. Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. IJSEM 57:2349-2354. All tests were carried out using medium MM1 containing (per liter distilled water) 0.5 g glucose, 0.04 g MgSO4.7H2O, 0.02 g CaCl2.2H2O and 0.1 g yeast extract.
An undefined organic chemical mixture derived from the juice of a tomato (the fruit of Solanum lycopersicum), formed through the process of maceration (homogenization) followed by filtration.
From: Mueller Hinton Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Each lot of Mueller Hinton Agar and Mueller Hinton II Agar has been tested according to, and meets the acceptance limits of, the current M6 protocol published by the CLSI. Mueller Hinton Agar is recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the Clinical and Laboratory Standards Institute (CLSI). Summary and Explanation Mueller Hinton Agar was originally developed for the cultivation of pathogenic Neisseria.6 However, these organisms are now commonly isolated on selective media. Principles of the Procedure Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenous substances, minerals, vitamins, carbon and other nutrients to support the growth of microorganisms. Starch acts as a protective colloid against toxic substances that may be present in the medium. Hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy. Agar is the solidifying agent. The Bauer-Kirby procedure is based on the diffusion through an agar gel of antimicrobial substances which are impregnated on paper discs.16 In contrast to earlier methods which used discs of high and low antimicrobial concentrations and which used the presence or absence of inhibition zones for their interpretation, this method employs discs with a single concentration of antimicrobial agent and zone diameters are correlated with minimal inhibitory concentrations (MIC).1,2,4,7,16 In the test procedure, a standardized suspension of the organism is swabbed over the entire surface of the medium. Paper discs impregnated with specified amounts of antibiotic or other antimicrobial agents are then placed on the surface of the medium, the plate is incubated and zones of inhibition around each disc are measured. The determination as to whether the organism is susceptible, intermediate or resistant to an agent is made by comparing zone sizes obtained to those in the CLSI Document M100(M2).4 Various factors have been identified as influencing disc diffusion susceptibility tests. These include the medium, excess surface moisture on the medium, agar depth, disc potency, inoculum concentration, pH and β-lactamase production by test organisms. 7,13,16 Formulae Difco™ Mueller Hinton Agar Approximate Formula* Per Liter Beef Extract Powder..................................................... 2.0 g Acid Digest of Casein................................................. 17.5 g Starch.......................................................................... 1.5 g Agar.......................................................................... 17.0 g pH 7.3 ± 0.1 BBL™ Mueller Hinton II Agar Approximate Formula* Per Liter Beef Extract.................................................................. 2.0 g Acid Hydrolysate of Casein......................................... 17.5 g Starch.......................................................................... 1.5 g Agar.......................................................................... 17.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.1
An organic-rich, solid microbiological culture medium containing beef extract, casein hydrolysate, and starch. Used for the cultivation of Neisseria.
An organic-rich, selective solid microbiological culture medium that contains peptones, lactose, bile salts, crystal violet, and a pH indicator (neutral red). The bile salts inhibit swarming in Proteus spp., and the crystal violet inhibits growth of some Gram-positive bacteria. Lactose fermentation lowers the pH, resulting in colonies that are red or pink in color, and causes the bile salts to precipitate. Non-lactose fermenters that can deaminate amino acids in the peptone release ammonia, which causes the pH to increase (and thus the colonies appear white).
From MacConkey Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use MacConkey agars are slightly selective and differential plating media mainly used for the detection and isolation of gram-negative organisms from clinical,1-3 dairy,4 food,5-7 water,8 pharmaceutical, 9-11 cosmetic,6,7 and other industrial sources. MacConkey Agar is used for isolating and differentiating lactose-fermenting from lactose-nonfermenting gram-negative enteric bacilli. Principles of the Procedure Peptones are sources of nitrogen and other nutrients. Yeast extract is a source of trace elements, vitamins, amino acids and carbon. Lactose is a fermentable carbohydrate. When lactose is fermented, a local pH drop around the colony causes a color change in the pH indicator (neutral red) and bile precipitation. Bile salts, bile salts no. 3, oxgall and crystal violet are selective agents that inhibit growth of gram-positive organisms. Sodium chloride maintains osmotic balance in the medium. Magnesium sulfate is a source of divalent cations. Agar is the solidifying agent. Formulae Difco™ MacConkey Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 17.0 g Peptones (meat and casein).......................................... 3.0 g Lactose...................................................................... 10.0 g Bile Salts No. 3............................................................. 1.5 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g Neutral Red.................................................................. 0.03 g Crystal Violet............................................................... 1.0 mg pH 7.1 ± 0.2 Difco™ MacConkey Agar Base Consists of the same ingredients without the lactose. BBL™ MacConkey Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 17.0 g Peptones (meat and casein).......................................... 3.0 g Lactose...................................................................... 10.0 g Bile Salts...................................................................... 1.5 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g Neutral Red.................................................................. 0.03 g Crystal Violet............................................................... 1.0 mg pH 7.1 ± 0.2
An organic-rich, solid microbiological culture medium that contains meat peptone, lactose and litmus (a pH indicator). Lactose fermenting bacteria will turn the pH indicator red as the pH decreases; colonies will be surrounded by a red zone. Colonies that turn blue are the result of the deamination of amino acids, resulting when the pH increases.
Vandamme P1, Segers P, Ryll M, Hommez J, Vancanneyt M, Coopman R, De Baere R, Van de Peer Y, Kersters K, De Wachter R, Hinz KH. 1998. Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. IJSB 48(2):431-440.
From Fluka Analytical: 62628 LL Agar (Litmus Lactose Agar according to Drigalski, Drigalski Litmus Lactose Agar) Selective medium for the differentiation of several bacteria based on lactose fermentation, in the inspection of water, milk, meat and other foodstuffs. Composition: Ingredients Grams/Litre Meat peptone 7.0 Sodium chloride 5.0 Lactose 15.0 Litmus 1.2 Agar 13.0 Final pH 7.4+/-0.2 at 37°C Store prepared media below 8°C, protected from direct light. Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C. Directions: Dissolve 41 g in 1 litre distilled water. Autoclave at 121°C for 15 minutes. Principle and Interpretation: LL Agar was formulated by Drigalski for the differentiation of Iactose fermenting and Iactose nonfermenting bacteria. It is importante for the detection of enteric pathogens in the inspection of water, milk, meat and other foodstuffs. Meat peptone provide nitrogenous nutrients to the organisms. Lactose is fermented by lactose fermenting bacteria with acid production. Litmus is the pH indicator which turns red at acidic pH. Colonies of the Iactose fermenting bacteria are surrounded by a red zone which distinguishes them from colonies of other organisms that either do not change the surrounding medium or make it blue due to production of ammonia. Inoculate culture from primary fermentation tubes (e.g. with Lactose Broth; Fluka 70142) showing gas production either by surface inoculation (using inoculation loop) or by pour plate method of serially diluted culture. Cultural characteristics after 24-72 hours at 35ºC .
Lecithovitellin Solution (Methods in Microbiology, v. 6, pt. 1, Chapter 1, Routine Biochemical Tests, pg 18): ...."which is made by adding 1 egg yolk to 225 mL of saline buffered with 0.1 M borate buffer at pH 7.2-7.4 with 0.005 M CaCl2; 10 g of a filtration aid such as Hyflo Supercel (Johns Manville Co., London) is added and the mixture shaken for 1 h before filtering twice through Whatman No. 1 papers and finally Seitz-filtering with negative pressure."
An organic-rich, solid microbiological culture medium based on nutrient agar, where lecithovitellin (a substance derived from the chemical treatment of chicken egg yolks) is added.
Recipe (from Cowan and Steel's Manual for the Identification of Medial Bacteria, 1993, ed. Barrow & Feltham, 3rd ed, pg. 205): Lecithovitellin Agar: Lecithovitellin solution (or egg yolk emulsion) 100 mL Nutrient agar 900 mL Melt the nutrient agar and cool to about 55˚C. Add the lecithovitellin solution aseptically, mix and pour plates.
An organic-rich, solid microbiological culture medium containing tryptone, yeast extract, in a base of synthetic sea water. Used to cultivate Tenacibaculum sp.
From: Suzuki M, Nakagawa Y, Harayama S, & Yamamoto S. 2001. Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. IJSEM 51:1639-1652. 2.0 g tryptone and 1.0 g yeast extract in 1000 mL Jamarin S synthetic sea water (Jamarin Laboratory) at pH 7.2
From: LB Broth, Miller (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Principles of the Procedure Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller. Formulae Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone.................................................................... 10.0 g Yeast Extract................................................................ 5.0 g Sodium Chloride........................................................ 10.0 g Agar.......................................................................... 15.0 g Difco™ LB Broth, Miller Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, liquid microbiological culture medium containing tryptone, yeast extract, and sodium chloride. Used to cultivate Escherichia coli.
From: LB Agar, Miller (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use LB Agar, Miller and LB Broth, Miller (Luria-Bertani) are used for maintaining and propagating Escherichia coli in molecular microbiology procedures. Principles of the Procedure Peptone provides nitrogen and carbon. Vitamins (including B vitamins) and certain trace elements are provided by yeast extract. Sodium ions for transport and osmotic balance are provided by sodium chloride. Agar is the solidifying agent in LB Agar, Miller. Formulae Difco™ LB Agar, Miller Approximate Formula* Per Liter Tryptone.................................................................... 10.0 g Yeast Extract................................................................ 5.0 g Sodium Chloride........................................................ 10.0 g Agar.......................................................................... 15.0 g Difco™ LB Broth, Miller Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, solid microbiological culture medium containing tryptone, yeast extract, and sodium chloride. Used to cultivate Escherichia coli.
From: http://medical-dictionary.thefreedictionary.com/laked+blood laked blood [lākt] Etymology: Fr, laque, a deep red color blood that is clear, red, and homogenous because of hemolysis of the red blood cells, as may occur in poisoning and severe extensive burns.
A solid microbiological culture medium (a blood agar), made with laked blood (where the red blood cells have been treated so that they have undergone haemolysis).
From: Shirling, E. B. & Gottlieb, D. (1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340. Medium 2: Yeast-extract - malt extract agar (Pridham et al., 1956-57) Bacto-Yeast Extract (Difco) 4.0g Bact-Malt Extract (Difco) 10.0 g Bacto-Dextrose (Difco) 4.0 g Distilled water 1.0 liter Adjust to pH 7.3, then add -- Bacto agar 20.0 g Liquefy agar by steaming at 100˚C for 15-20 minutes. Dispense appropriate amount for a slanting into at least 6 tubes for each culture. Sterilize by autoclaving; cool tubes as slants. Use the agar slants for preparation of stock cultures. Also sterilize medium 2 in flasks for pouring the sterilized medium into Petri dishes.
An organic-rich, solid microbiological culture medium, containing yeast extract, malt extract, and dextrose. Used to culture Streptomyces sp.
An organic-rich, solid microbiological culture medium containing water infusions of calf brains and beef hearts, proteose peptone, and dextrose (i.e. D-glucose).
From: Brain Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brain Heart Infusion (BHI) Agar is a general-purpose medium suitable for the cultivation of a wide variety of organism types, including bacteria, yeasts and molds. With the addition of 5% or 10% sheep blood, it is used for the isolation and cultivation of a wide variety of fungal species, including systemic fungi(1) from clinical and nonclinical sources. Principles of the Procedure BHI Agar derives its nutrients from the brain heart infusion, peptone and dextrose components. The peptones and infusion are sources of organic nitrogen, carbon, sulfur, vitamins and trace substances. Dextrose is a carbohydrate source that microorganisms utilize by fermentative action. The medium is buffered through the use of disodium phosphate. When defibrinated sheep blood is added to the basal medium, it provides essential growth factors for the more fastidious fungal organisms. Formulae Difco™ Brain Heart Infusion Agar Approximate Formula* Per Liter Calf Brains, Infusion from 200 g................................... 7.7 g Beef Heart, Infusion from 250 g................................... 9.8 g Proteose Peptone....................................................... 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 8.0 g Peptic Digest of Animal Tissue...................................... 5.0 g Pancreatic Digest of Casein........................................ 16.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 13.5 g pH 7.4 ± 0.2 BBL™ Brain Heart Infusion Agar, Modified Approximate Formula* Per Liter Brain Heart, Infusion from (solids)................................. 3.5 g Peptic Digest of Animal Tissue.................................... 15.0 g Pancreatic Digest of Casein........................................ 10.0 g Dextrose...................................................................... 2.0 g Sodium Chloride.......................................................... 5.0 g Disodium Phosphate.................................................... 2.5 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2
An organic-rich, solid microbiological culture medium that contains (beef) heart infusion and tryptose. Used to grow a wide range of heterotrophic microorganisms.
From: Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Heart Infusion Agar is a general-purpose medium used in the cultivation of a wide range of microorganisms from a variety of clinical and nonclinical specimens. Principles of the Procedure Heart Infusion Agar derives its nutrients from heart muscle infusion and peptone, which supply nitrogenous and carbonaceous compounds, sulfur, vitamins and trace ingredients. Sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. The addition of 5% sheep blood provides additional growth factors and is used to determine hemolytic reactions. Formula Difco™ Heart Infusion Agar Approximate Formula* Per Liter Beef Heart, Infusion from 500 g................................. 10.0 g Tryptose..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2
An organic-rich, liquid microbiological culture medium that contains (beef) heart infusion and tryptose. Used to grow a wide range of heterotrophic microorganisms.
From: Heart Infusion Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bacto™ Heart Infusion Broth is used for cultivating fastidious microorganisms. Principles of the Procedure Heart Infusion Agar derives its nutrients from heart muscle infusion and peptone, which supply nitrogenous and carbonaceous compounds, sulfur, vitamins and trace ingredients. Sodium chloride maintains osmotic equilibrium. Agar is the solidifying agent. The addition of 5% sheep blood provides additional growth factors and is used to determine hemolytic reactions. Formula Bacto™ Heart Infusion Broth Approximate Formula* Per Liter Beef Heart, Infusion from 500 g................................. 10.0 g Tryptose..................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2
An organic-rich, liquid microbiological culture medium containing water infusions of calf brains and beef hearts, proteose peptone, and dextrose (i.e. D-glucose).
From: Czapek-Dox Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Czapek-Dox Broth and Czapek Solution Agar are used for cultivating fungi and bacteria capable of using inorganic nitrogen. Principles of the Procedure Saccharose is the sole carbon source, and sodium nitrate is the sole nitrogen source in Czapek-Dox Broth and Czapek Solution Agar. Dipotassium phosphate is the buffering agent, and potassium chloride contains essential ions. Magnesium sulfate and ferrous sulfate are sources of cations. Agar is the solidifying agent in Czapek Solution Agar. Formulae Difco™ Czapek-Dox Broth Approximate Formula* Per Liter Saccharose................................................................. 30.0 g Sodium Nitrate............................................................. 3.0 g Dipotassium Phosphate................................................ 1.0 g Magnesium Sulfate...................................................... 0.5 g Potassium Chloride...................................................... 0.5 g Ferrous Sulfate............................................................. 0.01 g pH 7.3 ± 0.2
An organic-rich, liquid microbiological culture medium used for the cultivation of fungi and bacteria that can use saccharose (i.e. sucrose) as a sole carbon source and nitrate as a sole nitrogen source.
Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism.
From: Czapek-Dox Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Czapek-Dox Broth and Czapek Solution Agar are used for cultivating fungi and bacteria capable of using inorganic nitrogen. Principles of the Procedure Saccharose is the sole carbon source, and sodium nitrate is the sole nitrogen source in Czapek-Dox Broth and Czapek Solution Agar. Dipotassium phosphate is the buffering agent, and potassium chloride contains essential ions. Magnesium sulfate and ferrous sulfate are sources of cations. Agar is the solidifying agent in Czapek Solution Agar. Formulae Difco™ Czapek Solution Agar Approximate Formula* Per Liter Saccharose................................................................. 30.0 g Sodium Nitrate............................................................. 2.0 g Dipotassium Phosphate................................................ 1.0 g Magnesium Sulfate...................................................... 0.5 g Potassium Chloride...................................................... 0.5 g Ferrous Sulfate............................................................. 0.01 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2
An organic-rich, solid microbiological culture medium used for the cultivation of fungi and bacteria that can use saccharose (i.e. sucrose) as a sole carbon source and nitrate as a sole nitrogen source.
An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, yeast extract, phosphate buffer, glucose, maltose, cellobiose, starch, hemin and Vitamin K1. Used for the growth of anaerobes such as Clostridia.
From: Item AS-823, http://www.anaerobesystems.com/Home/pras-tubed-media/chopped-meat-carbohydrate-broth Formula Lean Ground Beef, 500.0 g Sodium Hydroxide (1N), 25.0 ml Pancreatic Digest of Casein, 30.0 g Yeast Extract, 5.0 g Potassium Phosphate, Dibasic, 5.0 g L-Cysteine, 0.5 g Hemin (0.1% Soln), 5.0 ml Vitamin K1 (1% Soln), 0.1 ml Dextrose, 4.0 g Maltose, 1.0 g Cellobiose, 1.0 g Starch, 1.0 g Distilled Water, 1000.0 ml Final pH 7.1 +/- 0.4 at 25 degrees C. Final volume 7.0 mL +/- 0.7.
An organic-rich, liquid microbiological culture medium containing typtose, dextrose (D-glucose), and sodium chloride. Used for the cultivation of Brucella.
From: Tryptose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptose Broth is used for cultivating Brucella and other fastidious microorganisms. Principles of the Procedure Tryptose peptone is a source of nitrogen and carbon. Dextrose is a source of carbohydrate. Sodium chloride maintains osmotic balance. Agar is the solidifying agent in Tryptose Agar. Formulae Difco™ Tryptose Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g Difco™ Tryptose Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2
From: Tryptose Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Tryptose Agar is used for cultivating a wide variety of fastidious microorganisms, particularly for isolating Brucella according to Huddleson and Castañeda. Principles of the Procedure Tryptose peptone is a source of nitrogen and carbon. Dextrose is a source of carbohydrate. Sodium chloride maintains osmotic balance. Agar is the solidifying agent in Tryptose Agar. Formulae Difco™ Tryptose Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2
An organic-rich, solid microbiological culture medium containing typtose, dextrose (D-glucose), sodium chloride and agar. Used for the cultivation of Brucella.
From Morgan WJB. 1961. The use of the thionin blue sensitivity test in the examination of Brucella. J Gen Microbiol 25:135-139. "Serum glucose agar was used for the proposation of cultures and as basal medium for the addition of dyes. The composition of this medium has been described (Morgan, 1960). Basic fuchsin (supplied by Pharmaceutical Laboratories National Aniline Division, Allied Chemical and Dye Corporation, New York) was added to the melted and cooled medium just before pouring plate to give a concentration of 1/25,000. Thionin (Allied Chemical and Dye Corp.) was used at a concentration of 1/50,000. Initially, three concentrations of thionin blue (British Drug Houses Ltd., London) were used, namely, 1/500,000, 1/1,000,000, 1/2,000,000. Later however only the 1/500,000 concentration was used for routine use. Stock solutions of the dyes were made and steam sterilized. All new batches were checked for activity against known strains. All plates were incubated overnight at 37˚ before use and no plate was used which had been stored for longer than one week in the refrigerator."
A tyrosine agar (or liver infusion agar, or glucose agar) supplemented with the pH indicator Basic Fuchsin (aka rosanilin, Basic Fuchsine, Rosaniline Hydrochloride, Magenta). If acid is produced, the basic fuchsin will turn deep red/pink.
From Huddleson IF. 1961. Emergence during growth of Brucella strains on dye-agar media of cells that show changes in sulfur metabolism. Bull Wld Hlth Org 24:91-102. "Initial differences in the growth of strains on agar containing thionin and basic fuchsin were determined by lightly inoculating the surface of tryptose agar in Petri plates containing separately 1 mg thionin/100 mL (basic aqueous solution 100 mg/100 mL) and 1 mg basic fuchsin/100 mL (basic ethanol solution 100 mg/100 mL). " pg. 94.
Tyrosine agar (or liver infusion agar, or glucose agar) with thionin blue stain (akas Thionin, Thionine, Thionine acetate, or Lauth's Violet) added.
From: Huddleson IF. 1961. Emergence during growth of Brucella strains on dye-agar media of cells that show changes in sulfur metabolism. Bull Wld Hlth Org 24:91-102. "Initial differences in the growth of strains on agar containing thionin and basic fuchsin were determined by lightly inoculating the surface of tryptose agar in Petri plates containing separately 1 mg thionin/100 mL (basic aqueous solution 100 mg/100 mL) and 1 mg basic fuchsin/100 mL (basic ethanol solution 100 mg/100 mL). " pg. 8.
From: Zaichikova MV, Berestovskaya YY, Kuznetsov BB, & Vasileva LV. 2013. Spirosoma xylofaga sp. nov., an oligotrophic pleomorphic bacterium from a myco-bacterial community of freshwater ecosystems. Microbiology 82(4):459-465. Pure culture was maintained in liquid HCY medium containing Hutner's basal salt solution (20 mL/L), a vitamin mixture [5], xylose (0.5 g/L) as a substrate, and yeast extract (0.01 g/L). [Ref 5]: Zaichikova, M.V., Berestovskaya, Akimov, V.N., Kizilova, A.K., and Vasileva, L.V., Xantobacter xylophilus sp. nov., a member of the xylotrophic myco-bacterial community of low mineral oligotrophic waters, Microbiology, 2010, vol. 79, no. 1, pp. 83-88.
From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=631 Hunter's basal salts: Nitrilotriacetic acid 10.0 g MgSO4·7H2O 14.45 g CaCl2·2H2O 4.42 g (NH4)6Mo7O24·4H2O 9.25 mg FeSO4·7H2O 99.0 mg Nicotinic acid 50.0 mg Thiamine·HCl 25.0 mg Biotin 0.5 mg Metals ''44'' (see Medium No. 149) 50.0 ml Distilled water 950.0 ml Dissolve and neutralized nitrilotriacetic acid in 500.0 ml distilled water with the addition of KOH. Add the remaining chemicals in order listed. Adjust pH to 6.8 and bring volume to 1.0 L with distilled water. From http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=149 Metals ''44'': EDTA·2Na 250.0 mg ZnSO4·7H2O 1095.0 mg FeSO4·7H2O 500.0 mg MnSO4·xH2O 154.0 mg CuSO4·5H2O 39.2 mg Co(NO3)2·6H2O 24.8 mg Na2B4O7·10H2O 17.7 mg Distilled water 1.0 L
A mineral-salts, liquid microbiological culture medium containing magnesium sulfate and nitrilotriacetic acid. Used to support the growth of Spirosoma and Xanthobacter.
From: Zaichikova MV, Berestovskaya YY, Kuznetsov BB, & Vasileva LV. 2013. Spirosoma xylofaga sp. nov., an oligotrophic pleomorphic bacterium from a myco-bacterial community of freshwater ecosystems. Microbiology 82(4):459-465. Pure culture was maintained in liquid HCY medium containing Hutners basal salt solution (20 mL/L), a vitamin mixture (5), xylose (0.5 g/L) as a substrate, and yeast extract (0.01 g/L). Ref 5: Zaichikova, M.V., Berestovskaya, Akimov, V.N., Kizilova, A.K., and Vasileva, L.V., Xantobacter xylophilus sp. nov., a member of the xylotrophic myco-bacterial community of low mineral oligotrophic waters, Microbiology, 2010, vol. 79, no. 1, pp. 83-88.
A mineral-salts, solid microbiological culture medium containing magnesium sulfate and nitrilotriacetic acid. Used to support the growth of Spirosoma and Xanthobacter.
An organic-rich, mineral-salts, liquid microbiological culture medium containing peptones and yeast extract in a base of artificial sea water. Diluted by half with water. Used for the cultivation of heterotrophic marine or brackish microorganisms.
From: Dextrose Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Dextrose Agar is used for cultivating a wide variety of microorganisms with or without added blood. Dextrose Broth is used for cultivating fastidious microorganisms and for detecting gas from enteric bacilli. Principles of the Procedure Beef extract and peptones provide nitrogen, amino acids and vitamins. Dextrose is a carbon source, and the increased concentration is a distinguishing characteristic of this medium from other formulations used as blood agar bases. Agar is the solidifying agent. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. Formulae Difco™ Dextrose Broth Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Proteose Peptone No. 3................................................ 2.0 g Pancreatic Digest of Gelatin......................................... 3.0 g Beef Extract.................................................................. 3.0 g Dextrose...................................................................... 5.0 g Sodium Chloride.......................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2
An organic-rich, liquid microbiological culture medium containing peptones and glucose. Used to culture a variety of microorganisms.
An organic-rich, solid microbiological culture medium containing peptones and glucose. Used to culture a variety of microorganisms.
From: Dextrose Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Dextrose Agar is used for cultivating a wide variety of microorganisms with or without added blood. Dextrose Broth is used for cultivating fastidious microorganisms and for detecting gas from enteric bacilli. Principles of the Procedure Beef extract and peptones provide nitrogen, amino acids and vitamins. Dextrose is a carbon source, and the increased concentration is a distinguishing characteristic of this medium from other formulations used as blood agar bases. Agar is the solidifying agent. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. Formulae Difco™ Dextrose Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Proteose Peptone No. 3................................................ 2.0 g Pancreatic Digest of Gelatin......................................... 3.0 g Beef Extract.................................................................. 3.0 g Dextrose.................................................................... 10.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2
An organic-rich, solid microbiological culture medium containing peptone, yeast extract, and acetate in a seawater base. Used to culture Flexibacter maritimus.
From Table 2 in: Pazos F, Santos Y, Macias AR, Nunez S, & Toranzo AE. 1996. Evaluation of media for the successful culture of Flexibacter maritimus. Journal of Fish Diseases 19:196-197. Components (g/L) Peptone 5.0 Yeast extract 0.5 Sodium acetate 0.01 Agar 15 In sea water as the diluent pH 7.2-7.4
From: McClung-Toabe Agar (Atlas, The Handbook of Microbiological Media for the Examination of Food, 2nd ed., pgs 221-222) Composition per liter: Proteose peptone 40.0g Agar 25.0g Na2HPO4 5.0 g Glucose 2.0 g NaCl 2.0 g KH2PO4 1.0 g MgSO4x7H2O 0.1 g Egg yolk emulsion, 50% 100.0 mL pH 7.3 +/- 0.2 at 25˚C Source: This medium is available as a pre-mixed powder from BD Diagnostic Systems. Egg Yolk Emulsion, 50%: Composition per 100.0 mL: Chicken egg yolks 11 Whole chicken egg 1 NaCl (0.9% solution) 50.0 mL Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100 dilution of saturated mercuric chloride solution for 1 min. Crack eggs and separate yolks from whites. Mix egg yolks with 1 chicken egg. Measure 50.0 mL of egg yolk emulsion and add to 50.0 mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45-50˚C. Preparation of Medium: Add components, except egg yolk emulsion, 50%, to distilled/deionized water and bring volume to 900.0 mL. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure -121˚C. Cool to 50-55˚C. Aseptically add 100.0 mL of sterile egg yolk emulsion, 50%. Mix thoroughly. Pour into sterile Petri dishes in 15.0 mL volumes. Use: For the isolation and cultivation of Clostridium perfringens in foods.
An organic-rich, liquid microbiological culture medium contining peptone, mineral-salts, glucose, and egg yolk emulsion. Used for the isolation and growth of Clostridium perfringens.
An organic-rich, solid microbiological culture medium containing peptones, phosphate buffer, blood, and glucose. Used for the growth of Bacteroides sp.
From: Eggerth AH & Gagnon BH. 1932. The bacteroides of human feces. J Bacteriol 35(4):389-413. "Our basic medium was beef infusion agar, containing 1.5 per cent of agar, 1 per cent of Parke Davis peptone, and 0.4 per cent of di-sodium phosphate. The pH was 7.6 to 7.8. Before pouring the plates, about 5 per cent of sterile blood and 0.15 per cent of sterile glucose (in the form of a 10 per cent solution) were added."
From: DNase Test Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use DNase Test Agar, DNase Test Agar with Methyl Green and DNase Test Agar with Toluidine Blue are differential media used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens. Summary and Explanation The DNase test is used to detect the degradation of deoxyribonucleic acid (DNA). The test is useful for differentiating Serratia from Enterobacter, Staphylococcus aureus from coagulase-negative staphylococci, and Moraxella catarrhalis from Neisseria species. Principles of the Procedure Peptones provide amino acids and other complex nitrogenous substances to support bacterial growth. Sodium chloride maintains osmotic equilibrium. DNA is the substrate for DNase activity. DNase is an extracellular enzyme that breaks the DNA down into subunits composed of nucleotides. The depolymerization of the DNA may be detected by flooding the surface of the medium with 1 N HCl and observing for clear zones in the medium surrounding growth. In the absence of DNase activity, the reagent reacts with the intact nucleic acid, resulting in the formation of a cloudy precipitate. The HCl reagent is not needed to detect DNase activity on DNase Agar with Methyl Green. Methyl green forms a complex with intact (polymerized) DNA to form the green color of the medium. DNase activity depolymerizes the DNA, breaking down the methyl green-DNA complex, which results in the formation of colorless zones around colonies of the test organism. A negative test is indicated by the absence of a colorless zone around the colonies. The HCl reagent is not needed to detect DNase activity on DNase Agar with Toluidine Blue. Toluidine blue forms a complex with intact (polymerized) DNA. In the intact DNA complex, the toluidine blue has the normal blue color. DNase activity depolymerizes the DNA, breaking down the dye-DNA complex. In the presence of nucleotides produced from the DNase depolymerization, the dye takes on its metachromatic color, forming pink to red zones around bacterial growth. A negative test is indicated when the medium remains blue. Formulae Difco™ DNase Test Agar Approximate Formula* Per Liter Tryptose..................................................................... 20.0 g Deoxyribonucleic Acid.................................................. 2.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2 BBL™ DNase Test Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean Meal..................................... 5.0 g Deoxyribonucleic Acid.................................................. 2.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 15.0 g pH 7.3 ± 0.2
An organic-rich, solid microbiological culture medium containing tryptose and DNA. Used to test for the ability of a microorganism to degrade DNA.
An organic-rich, solid microbiological culture medium containing peptone, lactose, citrate, deoxycholate, and neutral red. Used for the isolation of microorganisms that grow in milk (or other dairy products). Deoxycholate (a weak anionic detergent and bile acid) is used to lyse cells and solublize some cell membrane components. Inhibits growth of Gram positive bacteria, and therefore selects for Gram negative bacteria such as Salmonella.
From: Deoxycholate Lactose Agar (M066, HiMedia) Deoxycholate Lactose Agar is a differential and slightly selective medium used for the isolation and enumeration of coliforms in water, wastewater, milk and dairy products. Composition** Ingredients Gms / Litre Peptone, special 10.000 Lactose 10.000 Sodium chloride 5.000 Sodium citrate 2.000 Sodium deoxycholate 0.500 Neutral red 0.030 Agar 15.000 Final pH ( at 25°C) 7.1±0.2 **Formula adjusted, standardized to suit performance parameters Directions Suspend 42.53 grams in 1000 ml distilled water. Mix well and heat to boiling to dissolve the medium completely. The medium requires no autoclaving if it is to be used at once. If the medium is to be stored, it should be sterilized at 15 lbs pressure (121°C) for 15 minutes. AVOID OVERHEATING.
From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Mediumxxxx.pdf
From: Sawabe T, Makino H, Tatsumi M, Nakano K, Tajima K, Iqbal MM, Yumoto I, Ezura Y & Christen R. 1998. Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica. IJSB 48:769-774. "These isolated of Pseudoalteromonas bacteriolytica were maintained on CSY-3 agar medium containing casitone (Difco) 1.0 g, Difco bacto-soytone 1.0 g, Difco yeast extract 1.0 g, ferric ammonium citrate 0.1 g, and 1000 ml natural seawater, pH 7.5 (19). The stock cultures were maintained in CSY-3 broth containing 20 % glycerol (v/v)."
An organic-carbon enriched, marine, liquid microbiological culture medium containing peptones (casitone, soytone, yeast extract) and ferric ammonium citrate in a seawater base. Used for the cultivation of Pseudoalteromonas.
medium containing cellulose or cellulose derivatives
A microbiological culture medium containing carboxymethyl cellulose. Used to screen a culture for cellulolytic activity (the ability to hydrolyze cellulose, particularly those with endoglucanases).
http://medical-dictionary.thefreedictionary.com/Chocolate+Agar Blood agar that has been heated to open the pyrrole ring, forming hemin, a required growth factor for bacteria lacking hemolysins. Chocolate agars are usually incubated in a microaerophilic—3–10% CO2—environment, providing ideal growth conditions for H influenzae, Neisseria spp, and fastidious anaerobes
Wikipedia:Chocolate_agar Chocolate agar (CHOC) or chocolate blood agar (CBA) - is a non-selective, enriched growth medium. [1] [2] It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 °C. Chocolate agar is used for growing fastidious respiratory bacteria, such as Haemophilus influenzae and Neisseria meningitidis.[3] In addition, some of these bacteria, most notably H. influenza, need growth factors such NAD (factor V) and hemin (factor X), which are inside red blood cells; thus, a prerequisite to growth for these bacteria is lysis of the red blood cells. The heat also inactivates enzymes which could otherwise degrade NAD. The agar is named for the color and contains no actual chocolate.
A solid microbiological culture medium where blood has been been treated by heating in order to lyse the blood cells.
An organic-rich, solid microbiological culture medium that contains pancreatic digest of gelatin, potassium sulfate, and cetrimide (cetyl trimethyl ammonium bromide). A medium selective for Pseudomonas aeruginosa.
From: Cetrimide Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Cetrimide (Pseudosel) Agar is used for the selective isolation and identification of Pseudomonas aeruginosa. Principles of the Procedure Gelatin peptone supplies the nutrients necessary to support growth. The production of pyocyanin is stimulated by the magnesium chloride and potassium sulfate in the medium. Cetrimide is a quaternary ammonium, cationic detergent compound, which is inhibitory to a wide variety of bacterial species including Pseudomonas species other than P. aeruginosa. Agar is a solidifying agent. Cetrimide Agar Base is supplemented with 1% glycerol as a source of carbon. Formula Difco™ Cetrimide Agar Base Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 20.0 g Magnesium Chloride.................................................... 1.4 g Potassium Sulfate....................................................... 10.0 g Cetrimide (Tetradecyltrimethylammonium Bromide)...... 0.3 g Agar.......................................................................... 13.6 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2 Directions for Preparation from Dehydrated Product 1. Suspend 45.3 g of the powder in 1 L of purified water containing 10 mL of glycerol. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Test samples of the finished product for performance using stable, typical control cultures.
A trace elements solution containing boric acid, manganese chloride, ferrous sulfate, sodium tartrate, copper chloride, zinc chloride, cobalt chloride, and sodium molybdate.
From: http://www.atcc.org/~/media/DF06C418BB3A44189AD1A3CEB59B8D6E.ashx ATCC Medium: 1776 Tyrosine Agar (ISP Medium 7) Trace Elements Solution Ho-Le: H3BO3 2.85 g MnCl2x4H2O 1.8g FeSO4 1.36 g Sodium tartrate 1.77 g CuCl2x2H2O 26.9 mg ZnCl2 20.8 mg CoCl2x6H2O 40.4 mg Na2MoO4x2H2O 25.2 mg Distilled water 1.0 L
From: Brucella Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar is a culture medium for the cultivation of Brucella organisms. With the addition of 5% horse blood, the medium is used in qualitative procedures for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical specimens. Brucella Broth is used for the cultivation of Brucella species and for the isolation and cultivation of a wide variety of fastidious and nonfastidious microorganisms. Principles of the Procedure Brucella Agar and Brucella Broth support the growth of fastidious microorganisms due to their content of peptones, dextrose and yeast extract. The peptones supply organic nitrogen. The yeast extract is a potent source of the B-complex vitamins. Dextrose is utilized as an energy source. Sodium bisulfite is a reducing agent, and sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent in Brucella Agar. Formulae BBL™ Brucella Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Dextrose...................................................................... 1.0 g Yeast Extract................................................................ 2.0 g Sodium Chloride.......................................................... 5.0 g Sodium Bisulfite........................................................... 0.1 g Agar.......................................................................... 15.0 g BBL™ Brucella Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, liquid microbiological culture medium, containing pancreatic digest of casein (casitone), peptic digest of animal tissue (proteose peptone), dextrose (D-glucose), yeast extract, sodium chloride, and sodium bisulfite (sodium hydrogensulfite) as a reducing agent. Used for the growth of Brucella.
An organic-rich, solid microbiological culture medium, containing pancreatic digest of casein (casitone), peptic digest of animal tissue (proteose peptone), dextrose (D-glucose), yeast extract, sodium chloride, sodium bisulfite (sodium hydrogensulfite) as a reducing agent, and agar. Used for the growth of Brucella.
From: Brucella Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Brucella Agar is a culture medium for the cultivation of Brucella organisms. With the addition of 5% horse blood, the medium is used in qualitative procedures for the isolation and cultivation of nonfastidious and fastidious microorganisms from a variety of clinical and nonclinical specimens. Brucella Broth is used for the cultivation of Brucella species and for the isolation and cultivation of a wide variety of fastidious and nonfastidious microorganisms. Principles of the Procedure Brucella Agar and Brucella Broth support the growth of fastidious microorganisms due to their content of peptones, dextrose and yeast extract. The peptones supply organic nitrogen. The yeast extract is a potent source of the B-complex vitamins. Dextrose is utilized as an energy source. Sodium bisulfite is a reducing agent, and sodium chloride maintains the osmotic equilibrium. Agar is the solidifying agent in Brucella Agar. Formulae BBL™ Brucella Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Dextrose...................................................................... 1.0 g Yeast Extract................................................................ 2.0 g Sodium Chloride.......................................................... 5.0 g Sodium Bisulfite........................................................... 0.1 g Agar.......................................................................... 15.0 g BBL™ Brucella Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.0 ± 0.2
An organic-rich, solid microbiological culture medium containing casein and agar. Used to study microorganisms capable of growth in milk.
From: Ayers, SH. 1911. CASEIN MEDIA ADAPTED TO THE BACTERIAL EXAMINATION OF MILK. in U.S. Department of Agriculture. 28th annual report of the Bureau of Animal Industry. PREPARATION OF CASEIN-AGAR The method of preparation of casein-agar is as follows: PREPARATION OF ONE LITER. Casein solution 300 c.c. distilled water, 10 grams casein (Eimer & Amend C.P. casein prepared according to Hammarstan), 7 c.c. normal sodium hydroxid. After dissolving casein make up to 500 cubic centimeters. Agar solution. 500 c.c. distilled water, 10 grams agar. To 300 c.c. of water (distilled) add 10 grams casein (Eimer & Amend C.P. casein prepared according to Hammarsten) and 7 c.c. normal sodium hydroxid. Dissolve casein by heating to boiling. It is desirable to let this stand for several hours to get a perfect solution. This is not necessary, however. Make up volume to 500 c.c., and bring the reaction of the solution to between +0.1 and +0.2, Fuller's scale. Do not allow solution to become alkaline to phenolphthalein or over +0.2. If the casein is weighed accurately and the normal solution is accurate the reaction will be about +0.2. The agar solution is prepared by dissolving 10 grams of agar in 500 c.c. of water. Both casein and agar solutions should be filtered, then mixed. Tube and sterilize in autoclave under pressure for 20 minutes; then cool the tubes quickly in cold water or ice water. The final reaction of the medium will be about +0.1, Fuller's scale. IF the medium is alkaline the bacterial growth will be restricted. If the medium is more than +0.1 some of the casein may be precipitated during sterilization. The casein-agar should be clear and almost colorless when poured into a Petri dish. Sometimes the casein will be slightly precipitated during sterilization or the cooling, but this is of no consequence, since on pouring into plates the precipitate on account of its finely divided condition, becomes invisible.
A dilute, organic-carbon-containing liquid microbiological culture medium containing acetate, tryptone, and extracts of yeast and beef. Used for the cultivation and maintenance of Flexibacter psychrophilus.
ATCC medium 1750: Anacker and Ordal medium Tryptone (BD 211705).........0.5 g Yeast extract................0.5 g Sodium acetate...............0.2 g Beef extract.................0.2 g Agar........................10.0 g Distilled water..............1.0 L Adjust medium for final pH 7.3 +/- 0.1. Autoclave at 121C for 15 minutes. (For the broth the agar would be omitted).
A dilute, organic-carbon-containing solid microbiological culture medium containing acetate, tryptone, and extracts of yeast and beef. Used for the cultivation and maintenance of Flexibacter psychrophilus.
A mineral-salts, solid microbiological culture medium for the growth of freshwater and weakly brackish microorganisms that utilize methanol as a source of carbon.
From Handbook of Microbiological Media, 3rd edition: Composition per liter: Agar, 15.0 g MgSO4*7H2O, 1.0 g K2HPO4, 0.7 g KH2PO4, 0.54 g NH4Cl, 0.5 g CaCl2*2H2O, 0.2 g FeSO4*7H2O,4.0 mg H3BO4, 0.3 mg CoCl2*6H2O, 0.2 mg ZnSO4*7H2O, 0.1 mg Na2MoO4*4H2O, 0.06 mg MnCl2*4H2O, 0.03 mg NiCl2*6H2O, 0.02 mg CuCl2*2H2O, 0.01 mg pH 6.8 +/- 0.2 at 25˚C Preparation of Medium: Add components to distilled/deinionezed water and bring volume to 1.0 L. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure-121˚C. Add sterile methanol to a concentration of 0.5% aseptically to cooled basal medium. Use: for the cultivation and mantenance of bacteria that can utilize methanol as a carbon source, such as Methylobacterium species, Methylomonas species, and Methylophilus species.
Agar plates made up with washed red cells
A solid microbiological culture medium containing blood, where the red blood cells have been washed with a sterile saline solution.
From: http://medical-dictionary.thefreedictionary.com/washed+red+cells Washed Red Cells Transfusion medicine RBCs that have been washed in sterile saline to remove WBCs, lytic mediators, non-self antigens; WRCs are most useful in IgA-deficient Pts who have circulating anti-IgA antibodies, used to ↓ febrile, urticarial and anaphylactic reactions. See Blood filters.
Wikipedia:Berkefeld_filter A Berkefeld filter[1] is a oil filter made of diatomaceous earth or Kieselguhr. It was invented in Germany in 1891, and by 1922 was being marketed in the United Kingdom by the Berkefeld Filter Co.[2] Berkefeld was the name of owner of the mine in Hanover, Germany, where the ceramic material was obtained. The Berkefeld is a good bacterial water filter used in microbiological laboratories, in homes and out in the field.[3][4] Types The filters are classified according to the diameter of the pores in the ceramic material: V (Viel) - Coarsest pores N (Normal) - Intermediate sized pores W (Wenig) - Finest pores Usefulness The Berkefeld is a cheap, portable and efficient bacterial filter in general, though it does not remove viruses like Hepatitis A and some bacteria such as mycoplasma.[6] Some companies claim that they filter out from between 100% of particles above 0.9 micrometre to 98% of particles above 0.5 micrometre in diameter.[5] These are very durable filters and the filter elements may be cleaned over 100 times before requiring replacement. Some of the first Berkefeld filters were used during the 1892 cholera epidemic in Hamburg.[7]
A prokaryotic cell which has a filterable cell size that is small enough to pass through a Berkefeld filter candle with a defined pore size (e.g. V, N, or W), the largest of which is 0.45 microns.
From: Mudd S. 1923. The penetration of bacteria through capillary spaces. I. Motility and Size as Influencing Filterability through Berkefeld Candles. J Bacteriol 8(5):459-481. Table 4 (pg. 471) FILTER CALCULATED DIAMETER AVERAGE DIAMETER FOR FILTER TYPE micron micron V (10) 0.414 V (11) 0.380 V (16) 0.373 0.38 V (17) 0.376 V (18) 0.371 N (X) 0.461 N (XI) 0.437 0.45 W (1) 0.524 W (2) 0.47A W (X) 0.369 0.43 W (XI) 0.343
A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with a smaller pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms.
A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter.
A Berkefeld filterable cell that is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter.
An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, beef extract, oxgall, esculin, and ferric iron. Used for the differentiation of enterococci, Streptococcus bovis, and other streptococci,
From: Bile Esculin Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bile Esculin Agar is used to differentiate enterococci and the Streptococcus bovis group from other streptococci. Principles of the Procedure Enterococci and certain streptococci hydrolyze the glycoside esculin to esculetin and dextrose. Esculetin reacts with an iron salt to form a dark brown or black complex.6 Ferric citrate is incorporated into the medium as an indicator of esculin hydrolysis and resulting esculetin formation. Oxgall is used to inhibit gram-positive bacteria other than enterococci. Formula BBL™ Bile Esculin Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 5.0 g Beef Extract.................................................................. 3.0 g Oxgall........................................................................ 20.0 g Ferric Citrate............................................................... 0.5 g Esculin......................................................................... 1.0 g Agar ......................................................................... 14.0 g pH 6.8 ± 0.2
From: Wikipedia:Bile_esculin_agar Bile Esculin Agar (BEA) is a selective differential agar used to isolate and identify members of the genus Enterococcus, also known as "group D streptococci". Composition and process Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium. Test results must be interpreted in conjunction with gram stain morphology. Uses Bile Esculin Agar is used primarily to differentiate Enterococcus from Streptococcus. Members of the genus Enterococcus are capable of growing in the presence of 4% bile (oxgall) and hydrolyzing esculin to glucose and esculetin. Esculetin combines with ferric ions to produce a black complex. For some purposes, certain bacteria are able to hydrolyze aesculin. A plate containing aesculin will fluoresce a pale blue under UV radiation. Some bacteria can hydrolise this, leading to UV dark colonies, as opposed to UV light ones. When new techniques are produced to identify enterococci, they are often compared to the use of bile esculin agar.
A pancreatic digest of casein (milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms.
From Bacto™ Casitone (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Bacto™ Casitone is a pancreatic digest of casein. The manufacturing process for an enzymatic digest of casein is not as destructive as an acid hydrolysis. Thus, the casein is not broken down as completely into its constituent components. In many cases this makes for a more nutritious hydrolysate, especially for those organisms that prefer peptides to amino acids. Ash content is 6.4% NaCl content is 0.0%
From: http://himedialabs.com/TD/M883.pdf Meat Infusion Agar (Standard Infusion Agar) M883 (HiMedia Laboratories) Meat Infusion Agar is a nutritive medium used for mass cultivation of organisms for vaccine or toxin production. Composition** Ingredients Gms/Litre Peptic digest of animal tissue 10.000 Beef, infusion from 500.000 Sodium chloride 5.000 Agar 25.000 Final pH (at 25˚C) 7.5 +/- 0.2 ** Formula adjusted, standardized to suit performance parameters.
An organic-rich, solid microbiological culture medium containing peptic digest of animal tissue, beef infusion, and sodium chloride.
Gall (bile stored in the gall bladder) derived from an ox (a castrated adult male bovine; Bos taurus), used for the culturing of microorganisms.
From Wikipedia:Ox_gall: Ox gall is gall, usually obtained from cows, that is mixed with alcohol and used as the wetting agent in paper marbling, engraving, lithography, and watercolor painting. It is a greenish-brown liquid mixture containing cholesterol, lecithin, taurocholic acid, and glycocholic acid.
Bile salts derived from the bile of an ox (a castrated adult male bovine; Bos taurus). Used in the cultivation of microorganisms.
Bile derived from an ox (a castrated adult bovine; Bos taurus). Used for the culturing of microorganisms.
Undefined mixtures of complex organic compounds derived from the chopped meat (muscle and fat) of mammals, used in the cultivation of microorganisms.
Oil extracted from the germ (the plant embryo) of corn (Zea mays), used in the cultivation of microorganisms.
Microbiological medium ingredient, derived from chicken egg, where the yolks of chicken eggs have been homogenized (mixed).
An organic-rich, liquid microbiological culture medium that contains beef serum, heart infusion, whole egg, and dextrose. Used for the cultivation of Corynebacteria.
https://www.bd.com/ds/technicalCenter/inserts/L007463(07)(0107).pdf pH is 7.6 +/- 0.2.
Wikipedia:Loeffler's_medium Löffler's medium is a special substance used to grow diphtheria bacilli to confirm the diagnosis. History In 1887, Friedrich Loeffler devised a culture medium containing horse serum, meat infusion, and dextrose for use in the cultivation of corynebacteria and for differentiating them from other organisms.[1] Perry and Petran suggested modification of the original formulation.[2] Buck, in 1949, described a modified Loeffler's medium for cultivating Corynebacterium diphtheriae.[3] Uses This medium has a variety of uses in microbiological investigations. The current formulation incorporated these later modifications: The primary value of Loeffler medium is in the growth and morphological characterization of members of the genus Corynebacterium. This formulation enhances the formation of metachromatic granules within the cells of the organisms. Due to its serum content, Loeffler medium can be used for the determination of proteolytic activities of microorganisms. The gray-white surface of the medium provides an excellent background for the detection and observation of colonial pigmentation. If all extraneous moisture is removed aseptically from the slants and the upper part of the slant is heated until the slant ruptures, this medium can be used for the detection of ascospores. Principles of the procedure Heart muscle and animal tissue peptone provide the amino acids and other complex nitrogenous substances necessary to support growth of corynebacteria. Sodium chloride supplies essential ions. Dextrose is a source of fermentable carbohydrate. The eggs and beef serum cause the medium to coagulate during the sterilization process and are sources of protein which are used for metabolism of the corynebacteria and other organisms. Approximate formula Per liter purified water Beef serum 70.0 g Heart muscle, infusion from (solids) 0.72 g Peptic digest of animal tissue 0.71 g Sodium chloride 0.36 g Dextrose 0.71 g Egg (whole, dried) 7.5 g
anaerobic respiration, by disproportionation of thiosulfate
The process of anaerobic respiration, where thiosulfate is both oxidized to sulfate and reduced to hydrogen sulfide.
A gram stain quality where microorganisms stain purple with crystal violet used in the Gram stain.
A gram stain quality where microorganisms do not stain purple with Crystal Violet used in the Gram stain, but stain red due to uptake of the counter stain (Safranin or Fuchsin). Fuchsin is also called Magenta.
Microorganisms that are not definitively stained Gram positive or Gram negative; may stain variably as either Gram positive or Gram negative.
Movement (taxis) of a microorganism along the Earth's magnetic field lines.
Wikipedia: IMViC The methyl red test detects production of acids formed during metabolism using mixed acid fermentation pathway using pyruvate as a substrate. The pH indicator Methyl Red is added to one tube and a red color appears at pH's lower than 4.2, indicating a positive test (mixed acid fermentation is used). The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used.
Assays for the production of acids (e.g. pyruvate, lactate, acetate, formate) during the fermentation of glucose. The dye methyl red is red below pH 4.4 and yellow above pH 6.2. Acidic fermentation products result in a positive MR test giving a red result. A negative result is yellow.
Wikipedia: Mixed acid fermentation Mixed acid fermentation is the biological process by which a six-carbon sugar e.g. glucose is converted into a complex and variable mixture of acids. It is an anaerobic fermentation reaction that is common in bacteria. It is characteristic for members of the Enterobacteriaceae, a large family of Gram-negative bacteria that includes E. coli.[3] The mixture of end products produced by mixed acid fermentation includes lactate, acetate, succinate, formate, ethanol and the gases H2 and CO2. The formation of these end products depends on the presence of certain key enzymes in the bacterium. The proportion in which they are formed varies between different bacterial species.[4] The mixed acid fermentation pathway differs from other fermentation pathways, which produce fewer end products in fixed amounts. The end products of mixed acid fermentation can have many useful applications in biotechnology and industry. For instance, ethanol is widely used as a biofuel.[5] Therefore, multiple bacterial strains have been metabolically engineered in the laboratory to increase the individual yields of certain end products.[2] This research has been carried out primarily in E. coli and is ongoing.
An assay to determine if methylene blue can act as an artificial donor of electrons to cytochrome oxidase. When methylene blue is reduced it turns from oxidized blue form to the reduced colorless form. Methylene blue inhibits the growth of gram-positive bacteria, therefore inclusion in agar media is used to select for gram-negative bacteria.
An organic-rich, solid microbiological culture medium containing peptones, sodium chloride, and phenylethyl alcohol. Is a selective media that inhibits the growth of Gram-negative bacteria, and thus is selective for Gram-positive bacteria.
From: http://www.microbelibrary.org/library/laboratory-test/3653-phenylethyl-alcohol-agar-protocol Phenylethyl alcohol (bezylcarbinol) inhibits most gram-negative bacteria and fungi.
From: Phenylethyl Alcohol Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Phenylethyl Alcohol (PEA) Agar is a selective medium for the isolation of gram-positive organisms, particularly gram-positive cocci, from specimens of mixed gram-positive and gram-negative flora.1 The medium, when supplemented with 5% sheep blood, should not be used for determination of hemolytic reactions since atypical reactions may be observed. Principles of the Procedure Phenylethyl Alcohol Agar and Phenylethyl Alcohol Agar with 5% Sheep Blood support the growth of gram-positive bacterial species, due to the content of peptones, which supply nitrogen, carbon, sulfur and trace nutrients. Sodium chloride maintains osmotic equilibrium. Sheep blood is a source of growth factors. Phenylethyl alcohol is bacteriostatic for gramnegative bacteria since it selectively and reversibly inhibits DNA synthesis.3 Formula BBL™ Phenylethyl Alcohol Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 15.0 g Papaic Digest of Soybean Meal..................................... 5.0 g Sodium Chloride.......................................................... 5.0 g β-Phenylethyl Alcohol................................................... 2.5 g Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2
From: Mannitol Salt Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Mannitol Salt Agar is used for the selective isolation and enumeration of staphylococci from clinical and nonclinical materials. Principles of the Procedure Mannitol Salt Agar is a nutritive medium due to its content of peptones and beef extract, which supply essential growth factors, such as nitrogen, carbon, sulfur and trace nutrients. The 7.5% concentration of sodium chloride results in the partial or complete inhibition of bacterial organisms other than staphylococci. Mannitol fermentation, as indicated by a change in the phenol red indicator, aids in the differentiation of staphylococcal species. Agar is a solidifying agent. Formula BBL™ Mannitol Salt Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 5.0 g Peptic Digest of Animal Tissue...................................... 5.0 g Beef Extract.................................................................. 1.0 g Sodium Chloride........................................................ 75.0 g D-Mannitol................................................................ 10.0 g Phenol Red................................................................ 25.0 mg Agar.......................................................................... 15.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2
A hypersaline, organic-rich, solid microbiological culture medium that contains peptones, 7.5% sodium chloride, D-mannitol, and a pH indicator (phenol red). Mannitol fermentation lowers the pH, causing the red pH indicator to turn yellow. Used to distinguish between strains of Staphylococcus.
Methylene blue acts as an artificial donor of electrons to cytochrome oxidase. When reduced it turns from oxidized blue form to the reduced colorless form.
Methylene blue inhibits the growth of gram-positive bacteria, therefore inclusion in agar media is used to select for gram-negative bacteria.
From: Eosin Methylene Blue Agar, Levine (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eosin Methylene Blue Agar, Levine is a slightly selective and differential plating medium for the isolation of gram-negative enteric bacteria. EMB Agar, Levine, without Lactose is provided for convenience in genetic studies of enteric bacilli. Principles of the Procedure The eosin Y and methylene blue dyes in Levine EMB Agar render the medium slightly selective in that they inhibit gram-positive bacteria to a limited degree. These dyes also play a role in differentiating between lactose fermenters and lactose nonfermenters due to the presence or absence of dye uptake in the bacterial colonies. Coliforms, as lactose-fermenting organisms, are visualized as blue-black colonies, whereas colonies of Salmonella and Shigella, as lactose nonfermenters, appear colorless, transparent or amber. Some gram-positive bacteria, such as fecal streptococci, staphylococci and yeasts, will grow on this medium and usually form pinpoint colonies. A number of nonpathogenic, lactose-nonfermenting gram-negative bacteria will grow on this medium and must be distinguished from the pathogenic strains by additional biochemical tests. Formulae BBL™ Eosin Methylene Blue Agar, Levine Approximate Formula* Per Liter Pancreatic Digest of Gelatin....................................... 10.0 g Lactose...................................................................... 10.0 g Dipotassium Phosphate................................................ 2.0 g Eosin Y........................................................................ 0.4 g Methylene Blue.......................................................... 65.0 mg Agar.......................................................................... 15.0 g BBL™ EMB Agar, Levine, without Lactose Consists of the same ingredients without the lactose. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.1 ± 0.2
An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, lactose, and dyes (Eosin Y and Methylene Blue) as selective agents. The dyes slightly inhibit the growth of Gram-positive bacteria. They are also used to differentiate lactose fermenters from those that do not ferment lactose based on the uptake of dye (lactose fermenters take up dye and appear blue-black).
A group of diagnostic metabolic tests used to differentiate coliform microorganisms. Includes indole test, methyl red test, Voges-Proskauer test, and citrate test.
An assay used to test for the production of acetoin by a microorganism as a product of glucose metabolism. Voges-Proskauer broth (containing alpha-naphthol and KOH) is inoculated with the test strain. A red color indicates a positive result for acetoin; absence of acetoin yields a yellow-brown color and indicates a negative result.
Uses Simmons' citrate agar containing citrate. Microorganisms able to metabolize citrate change the pH of the medium through the production of sodium bicarbonate and ammonia, resulting in an alkaline pH (resulting in a change in the pH indicator, bromthymol blue). A positive reaction (alkaline pH) results in a blue (or blue-green) color; a negative reaction (neutral pH) results in a green (or pale green/yellow) color.
An organic-rich, liquid medium that contains peptones, glucose, and a phosphate pH buffer. After growth, the Methyl Red test is performed by adding methyl red and looking for a color change. If glucose is fermented to acid sufficient to overcome the pH buffer, a red color will result. After growth, the Voges-Proskauer test is performed by adding KOH and alpha-naphthol to the culture. If acetoin was produced from glucose fermentation, a red color will result.
From: MR-VP Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use MR-VP Medium and MR-VP Broth (Methyl Red-Voges Proskauer Medium/Broth, also known as Buffered Peptone- Glucose Broth) are used for the differentiation of bacteria by means of the methyl red and Voges-Proskauer reactions. Principles of the Procedure Methyl red-positive organisms produce high levels of acid during fermentation of dextrose, overcome the phosphate buffer system and produce a red color upon the addition of the methyl red pH indicator. In the Voges-Proskauer test, the red color produced by the addition of potassium hydroxide to cultures of certain microbial species is due to the ability of the organisms to produce a neutral end product, acetoin (acetylmethylcarbinol), from the fermentation of dextrose.3 The acetoin is oxidized in the presence of oxygen and alkali to produce a red color.3 This is a positive Voges-Proskauer reaction. Formulae Difco™ MR-VP Medium Approximate Formula* Per Liter Buffered Peptone......................................................... 7.0 g Dipotassium Phosphate................................................ 5.0 g Dextrose...................................................................... 5.0 g pH 6.9 ± 0.2 BBL™ MR-VP Broth Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 3.5 g Peptic Digest of Animal Tissue...................................... 3.5 g Potassium Phosphate................................................... 5.0 g Dextrose...................................................................... 5.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.2
A mineral-salts, solid microbiological culture medium containing phosphate buffer, sodium chloride, sodium citrate, magnesium sulfate, and a pH indicator (bromthymol blue). Used to test for the metabolism of citrate.
From: Simmons Citrate Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Simmons Citrate Agar is used for the differentiation of gramnegative bacteria on the basis of citrate utilization. Principles of the Procedure Organisms able to utilize ammonium dihydrogen phosphate and sodium citrate as the sole sources of nitrogen and carbon, respectively, will grow on this medium and produce an alkaline reaction as evidenced by a change in the color of the bromthymol blue indicator from green (neutral) to blue (alkaline). Formula BBL™ Simmons Citrate Agar Approximate Formula* Per Liter Ammonium Dihydrogen Phosphate.............................. 1.0 g Dipotassium Phosphate................................................ 1.0 g Sodium Chloride.......................................................... 5.0 g Sodium Citrate............................................................. 2.0 g Magnesium Sulfate...................................................... 0.2 g Agar.......................................................................... 15.0 g Bromthymol Blue......................................................... 0.08 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.2
freshwater microbiological culture medium
A microbiological culture medium that has a salinity of less than 0.05 % salts.
An organic-rich, liquid microbiological culture medium containing peptones, ferrous iron, and thiosulfate. Used to test for the production of sulfide and indole, and motility in a microorganism.
From: http://www.microbelibrary.org/library/2-associated-figure-resource/3645-sim-medium Sulfide Indole Motility Medium Sulfide indole motility (SIM) medium is a semisolid agar used to determine hydrogen sulfide (H2S) production, indole formation, and motility. SIM medium is used to differentiate members of the family Enterobacteriaceae. Haziness that spreads from the stab line indicates a positive test for motility. Tubes must be compared to an uninoculated tube to discriminate between faint haziness and motility. A red color development after addition of Kovács reagent indicates indole production. A black precipitate indicates H2S production. Test tubes: (A) uninoculated tube, (B) contains the nonmotile and indole-negative bacterium Klebsiella pneumoniae, (C) contains the motile and indole-positive bacterium Escherichia coli, and (D) contains the motile, indole-negative, and H2S-producing bacterium Proteus mirabilis. (Renee Wilkins, University of Mississippi Medical Center, Jackson, MS)
From: SIM Medium (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use SIM Medium is used to differentiate enteric bacilli on the basis of sulfide production, indole formation and motility. Principles of the Procedure The ingredients in SIM Medium enable the determination of three activities by which enteric bacteria can be differentiated. Sodium thiosulfate and ferrous ammonium sulfate are indicators of hydrogen sulfide production. The ferrous ammonium sulfate reacts with H2S gas to produce ferrous sulfide, a black precipitate.1 The casein peptone is rich in tryptophan, which is attacked by certain microorganisms resulting in the production of indole. The indole is detected by the addition of chemical reagents following the incubation period. Motility detection is possible due to the semisolid nature of the medium. Growth radiating out from the central stab line indicates that the test organism is motile. Formula BBL™ SIM Medium Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 20.0 g Peptic Digest of Animal Tissue...................................... 6.1 g Ferrous Ammonium Sulfate.......................................... 0.2 g Sodium Thiosulfate...................................................... 0.2 g Agar............................................................................ 3.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.3 ± 0.2
An assay to determine hydrogen sulfide production, indole production, and motility of a microorganism. For the assay, an inoculum is made into sulfide indole motility (SIM) medium, an agar medium allowing three simultaneous assays to be performed: 1) hydrogen sulfide production due to cyteine desulfurase (indicated by a black precipitate) - the hydrogen sulfide test 2) indole production due to tryptophanase (indicated by red color development due to Kovacs reagent in the indole test) - the indole test 3) motility (spread of microbial cells away from the inoculum)
Filament branch quality defining the shape of the filament branch.
branch diameter thickness relative to main filament
Filament branch length defining the filament branch thickness relative to that of the main filament.
Filament branch size where the diameter of cylindrical cells in the filament branches are thinner than the diameter of cylindrical cells in the main trichome.
Filament branch physical object quality relating to the spatial pattern (symmetry and position) of branches in relation with each other and with the long axis of the main filament, imparting some symmetry or asymmetry to the filament.
Filament branch spatial pattern defining the angle between a filament (i.e., the main trichome) and a filament branch.
Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is subperpendicular (forming an obtuse angle).
Filament branch angle, where the angle between the cylindrical cells of the branching filament and the cylindrical cells of the main trichome is subparallel (forming an acute angle).
From: Urea Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Urea Agar and Urease Test Broth are used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Principles of the Procedure The urea medium of Rustigian and Stuart3 is particularly suited for the differentiation of Proteus species from other gramnegative enteric bacilli capable of utilizing urea;1 the latter are unable to do so in Urease Test Broth because of limited nutrients and the high buffering capacity of the medium. To provide a medium with greater utility, Urea Agar was devised by Christensen1 with peptone and dextrose included and reduced buffer content to promote more rapid growth of many of the Enterobacteriaceae and permit a reduction in incubation time. The complete Urea Agar contains 15.0 g/L of agar in addition to the ingredients in the base medium. When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a red-pink color. Consequently, urease production may be detected by the change in the phenol red indicator. Formulae Difco™ Urea Broth Approximate Formula* Per Liter Yeast Extract................................................................ 0.1 g Monopotassium Phosphate.......................................... 9.1 g Dipotassium Phosphate................................................ 9.5 g Urea........................................................................... 20.0 g Phenol Red.................................................................. 0.01 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.1
An organic-rich, liquid microbiological culture medium containing pancreatic digest of gelatin, dextrose (D-glucose), sodium chloride, phosphate, urea, and a pH indicator (phenol red).
An organic-rich, solid microbiological culture medium containing pancreatic digest of gelatin, dextrose (D-glucose), sodium chloride, phosphate, urea, a pH indicator (phenol red) and agar.
From: Urea Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Urea Agar and Urease Test Broth are used for the differentiation of organisms, especially the Enterobacteriaceae, on the basis of urease production. Principles of the Procedure The urea medium of Rustigian and Stuart3 is particularly suited for the differentiation of Proteus species from other gramnegative enteric bacilli capable of utilizing urea;1 the latter are unable to do so in Urease Test Broth because of limited nutrients and the high buffering capacity of the medium. To provide a medium with greater utility, Urea Agar was devised by Christensen1 with peptone and dextrose included and reduced buffer content to promote more rapid growth of many of the Enterobacteriaceae and permit a reduction in incubation time. The complete Urea Agar contains 15.0 g/L of agar in addition to the ingredients in the base medium. When organisms utilize urea, ammonia is formed during incubation which makes the reaction of these media alkaline, producing a red-pink color. Consequently, urease production may be detected by the change in the phenol red indicator. Formulae BBL™ Urea Agar Base Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 1.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Potassium Phosphate................................................... 2.0 g Urea........................................................................... 20.0 g Phenol Red................................................................ 12.0 mg pH 6.8 ± 0.1
Assay for the presence of cytochrome oxidase (aka indophenol oxidase) in a microbiological culture. Uses a colorometric dye (tetra-methyl-p-phenylenediamine) as an electron donor for cytochrome c. When reduced the dye is colorless, when oxidized by cytochrome oxidase it becomes dark blue/purple. There are many variations of the test. It can be done on treated filter paper, on plates, or in a test tube using a fresh culture grown in broth.
Test for the presence of nitrate reductase in a microbial culture. Nitrite reacts with sulfanilic acid under acidic conditions (5N acetic acid) to produce (colorless) diazotized sulfanilic acid. This sulfanilic acid is then reacted to (colorless) alpha-naphthylamine (or N,N-dimethyl-alpha-naphthylamine) producing a red water soluble azo dye (p-sulfobenzene-azo alpha-naphthylamine). Presence of nitrite then results in a pink/red color (a positive test result). Zinc dust can be used to chemically (abiotically) reduce any nitrate remaining in the test to nitrite (to test for the presence of unreduced nitrate in the medium). If nitrate has been completely reduced to nitrogen or ammonia, zinc dust will result in no color development. This is a postive test result. However, if nitrate was not reduced and remains in the media, zinc will reduce it to nitrite resulting in a red color. This is a negative test result.
An enzymatic assay which tests for the presence of catalase in a microorganism. The catalase reaction converts hydrogen peroxide into water and oxygen: 2H2O2 -> 2 H2O + O2 For the catalase test a drop of 3% or 15% hydrogen peroxide is in contact with cells and the appearance of oxygen bubbles indicates a positive test result. No bubbles indicates a negative test result. There are several variants of the test: on a slide (where cells are smeared onto the glass), in a test tube where cells are placed into a hydrogen peroxide solution), an agar slant, or a colony on a plate.
Assays for the ability of an organism to break down the protein casein. Usually tested by incorporating casein into agar. A positive test will result in clearing of the agar due to degradation of the opaque casein. Commonly uses Milk Agar or Casein Agar.
A chemcial sensitivity assay for the ability to grow in the presence of digitonin, a glycoside compound that solubilizes lipids and cell membranes.
An assay for the presence of gelatinase in a microorganism. Gelatinase is a proteolytic enzyme that breaks down gelatin (a partially hydrolyzed form of collagen derived from animal tissue that is comprised of peptides and proteins) via a hydrolysis reaction. Gelatin hydrolysis (a positive reaction) is found when the liquefaction of a gelatin-containing substrate is observed.
An enzymatic assay which tests for the presence of phosphatases in a microorganism, using various chromogenic substrates.
An assay for the ability of a microorganism to reduce resazurin. Resazurin is a dye in which under oxidizing conditions it is blue, however when reduced becomes pink (it is also strongly fluorescent). It functions as a redox indicator, and in viability tests.
In the tellurite reduction test, tellurite is reduced to metallic tellurium, which is dark grey. Some microorganisms are resistant to tellurite, and thus tellurite reduction in those organisms is an assay of redox (respiratory) metabolism. The extent of tellurite reduction is also a measure of the extent of respiratory metabolism. Tellurite added to plates, when reduced, generates colonies that are dark grey in color. Tellurite is toxic, and so may be inhibitory at concentrations ranging from 40-120 mg/L. Nagai S. 1965. Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutans. J Bact 90(1):220-222.
API® 32 Staph; Identification system for staphylococci ; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
suite of microbiological diagnostic tests
A set of assays that are sold as diagnostic/identification kits that are purchased that can identify commonly isolated microorganisms.
Test kits old by bioMerieux, Inc. (www.biomerieux.com/en)
API = analytical profile index, a system for the identification of microorganisms. API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Humble MW, King A, Phillips I. 1977. API ZYM: a simple rapid system for the detection of bacterial enzymes. J. Clin. Path. 30:275-277.
API ZYM® – Semiquantification of enzymatic activities; http://www.biomerieux-usa.com; System for the research of enzymatic activity API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API® 20E – 18-24 hour identification of Enterobacteriacae and other non-fastidious gram negative bacteria; http://www.biomerieux-usa.com API, and API 20E, are a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API® 50 CH – Performance of carbohydrate metabolism tests; http://www.biomerieux-usa.com API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API® Rapid ID 32 A – 4-hour identification of anaerobes; http://www.biomerieux-usa.com; Identification system for anaerobes API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API 20A® – 24-hour identification of anaerobes; http://www.biomerieux-usa.com; System for the identification of anaerobes API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API® ID 32 GN; Automatic identification system for Gram-negative rods; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
API 20NE® – 24 to 48-hour identification of Gram negative non-Enterobacteriaceae; http://www.biomerieux-usa.com API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
N-acetyl-beta-glucosaminidase assay with naphthol
An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 1-naphthyl-N-acetyl-beta-D-glucosaminide at pH 5.4. N-acetyl-beta-glucosaminidase activity (catalyzing the hydrolysis of terminal beta-N-acetylglucosamine residues from oligosaccharides) will cleave the substrate, producing 1-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is brown in color.
A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism using the substrate 6-bromo-2-naphthyl-beta-D-mannopyranoside at pH 5.4. beta-Mannosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-D-mannose residues in beta-D-mannosides) will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo substituted azo dye) that is purple in color.
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-beta-D-glucopyranoside at pH 5.4. beta-Glucosidase activity will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color.
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 2-naphthyl-beta-D-glucopyranoside at pH 5.4. beta-Glucosidase activity will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color.
beta-glucuronidase assay with naphthol AS-BI
An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 2-naphthol AS bis-beta-D-glucuronide (naphthol AS-BI beta-D-glucuronide, O-beta-D-glucuronosyl-naphtol AS-BI, naphthol ASBI-glucuronic acid) at pH 5.4. beta-Glucuronidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is blue in color. A positive test yields a blue color; a negative test is colorless, pale gray, or pale beige.
An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 2-naphthyl-beta-D-galactopyranoside at pH 5.4. beta-Galactosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color.
beta-galactosidase assay with BrNaphthol
An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-beta-D-galactopyranoside at pH 5.4. beta-Galactosidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color.
Uses the substrate naphthol AS BI-phosphate (aka naphthol AS bis-phosphodiamide, or 7-bromo-3-hydroxy-2-naphthoic-o-anisidide phosphate) at pH 5.4. Naphthol-AS-BI-phosphohydrolase will cleave the substrate, producing 7-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is blue in color. A phosphoamidase enzyme is a hydrolase enzyme that acts on phosphorus-nitrogen bonds, releasing phosphate. For example: N-phosphocreatine + H2O <=> creatine + phosphate
Uses the substrate 2-naphthyl phosphate at pH 5.4. Acid phosphatase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
An assay for the presence of chymotrypsin in a microorganism. When the chemical substrate of chymotrypsin is cleaved by the enzyme, a colored produce is released which is then measured using spectrophotometry.
An assay for the presence of trypsin in a microorganism. When the chemical substrate of trypsin is cleaved by the enzyme, a colored produce is released which is then measured using spectrophotometry.
zzzz di-napthylamide is not napthylamide
An alpha-amino acid arylamidase assay that uses the substrate L-cystine-di-2-naphthylamide at pH 7.5. Cystine arylamidase activity (which could be from cystine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. The presence of thiols converts this compound to L-cysteine-2-naphthylamide. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-valyl-2-naphthylamide at pH 7.5. Valine arylamidase activity (which could be from valine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-leucyl-2-naphthylamide (L-leucine-beta-naphthylamide) at pH 7.5. Leucine arylamidase activity (which could be from leucine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Uses the substrate 2-naphthyl myristate at pH 7.1. Esterases that can hydrolyze C14 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
Uses the substrate 2-naphthyl caprylate at pH 7.1. Esterases that can hydrolyze C8 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
alkaline phosphatase assay with naphthol
Alkaline phosphatase assay that uses the substrate 2-naphthyl phosphate at pH 8.5. Alkaline phosphatase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
Test for C4 esterase in a microorganism. Uses the substrate 2-naphthyl butyrate at pH 7.1. Esterases that can hydrolyze C4 compounds will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
An assay for the activity of beta-L-fucosidase in a microorganism. Uses the substrate 2-naphthyl-beta-L-fucoside at pH 5.4. beta-Fusosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-L-fucose residues in beta-fucosides) will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color.
alanyl phenylalanyl proline arylamidase assay
alanyl phenylalanyl proline aminopeptidase
An assay for alanyl phenylalanyl proline arylamidase activity. Uses the substrate L-alanyl-L-phenylalanyl-L-proline-2-naphthylamide at pH 7.5. Alanyl phenylalanyl proline arylamidase activity (which could be from alanyl phenylalanyl proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An assay for the activity of beta-galactosidase in a microorganism. This assay measures beta-galactosidase activity using the colorless substrate ONPG (ortho-nitrophenyl-beta-D-galactoside, 2-nitrophenyl beta-D-galactopyranoside). beta-Galactosidase cleaves the substrate producing galactose and ortho-nitrophenol (yellow). A positive test yields a yellow color; a negative test is colorless. This test requires an additional inducer of transcription of the lac operon (such as IPTG; isopropyl beta-D-1-thiogalactopyranoside).
decarboxylation of the amino acid arginine by arginine dihydrolase
The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine dihydrolase (arginine deiminase) catalyzes the reaction: L-arginine + H2O <=> L-citrulline + NH3
As assay to determine if a microbial isolate is capable of metabolizing lysine under anaerobic conditions. When lysine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. cadaverine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Lysine decarboxylase catalyzes the following reaction: L-lysine <=> cadaverine + CO2
"Lysine dihydrolase" test results found in a small number of prokaryotic taxonomic descriptions is likely an error - it is more likely to be "lysine decarboxylase" test results.
decarboxylation of the amino acid lysine by lysine decarboxylase (sic)
decarboxylations of the amino acid ornithine by ornithine decarboxylase (sic)
The purpose of this test is to determine if a microbial isolate is capable of metabolizing ornithine under anaerobic conditions. When ornithine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Ornithine decarboxylase catalyzes the reaction: L-ornithine <=> putrescine + CO2
The purpose of this assay is to determine if a microbial isolate is capable of metabolizing tryptophan under anaerobic conditions, via the enzyme L-tryptophan aminotransferase (L-tryptophan transaminase). Tryptophan is deamined to form indolepyruvic acid (indole-3-pyruvate, an alpha keto acid) and ammonia. When FeCl3 (ferric chloride) is added, it reacts with the indolepyruvic acid with the presence of hydrazine compounds in the culture media to produce a brown/red precipitate. A positive test yields a brown/red color; a negative test is yellow. L-tryptophan aminotransferase catalyzes the following reaction: L-tryptophan + 2-oxoglutarate <-> indole-3-pyruvate + L-glutamate
API commercial test for the presence of gelatinase in a microorganism. Charcoal is added to gelatin beads. If gelatinase is present, the gelatin will be liquified and the charcoal released in to the solution. A positive result shows diffusion of black pigment (charcoal); a negative result shows no diffusion of charcoal, with intact gelatin beads.
organic molecular entity fermentation/oxidation assay
Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be fermented or oxidized by a microorganism. The bottom of the reaction chamber becomes anaerobic where fermentation can occur, whereas the top of the chamber is in contact with oxygen and therefore is aerobic. Fermentation of a neutral carbohydrate substrate results in acidic condition, or a yellow color. A positive result is yellow; a negative result is blue or blue-green. --------------------------------------- Metabolism of an organic acid results in a transition from acidic to neutral pH. A positive result is green to blue; a negative result is yellow.
Sodium, Potassium, Calcium, or Magnesium ions are required to be present in order for growth of a particular microorganism to occur.
Assays for the ability of a microorganism to ferment or oxidize D-glucose.
Assays for the ability of a microorganism to ferment or oxidize D-mannitol.
Assays for the ability of a microorganism to ferment or oxidize inositol.
Assays for the ability of a microorganism to ferment or oxidize D-sorbitol (D-glucitol).
Assays for the ability of a microorganism to ferment or oxidize L-rhamnose.
Assays for the ability of a microorganism to ferment or oxidize sucrose.
beta-melibiose fermentation/oxidation assay
Assays for the ability of a microorganism to ferment or oxidize D-melibiose (beta-melibiose).
Assays for the ability of a microorganism to ferment or oxidize amygdalin.
L-arabinose fermentation/oxidation assay
Assays for the ability of a microorganism to ferment or oxidize L-arabinose.
A test using triphenyl tetrazolium chloride (2,3,5-triphenyl-2H-tetrazolium chloride, tetrazolium chloride, TTC) is a redox indicator used to quantify respiration. Actively respiring cells will reduce TTC (clear/white) to insoluble TPF (1,3,5-triphenylformazan) which is a red color. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink.
aerobic triphenyl tetrazolium reduction assay
Assays the ability of a microorganism to reduce triphenyl tetrazolium chloride under aerobic conditions (using aerobic respiration). The color change (production of the red reduced product will occur near the top of the cupule where oxygen is present. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink.
anaerobic triphenyl tetrazolium reduction assay
Assays the ability of a microorganism to reduce triphenyl tetrazolium chloride under anaerobic conditions (using anaerobic respiration). The color change (production of the red reduced product will occur near the bottom of the cupule where oxygen is absent. A positive test result is pink red (or results in a deposit in the base of the cupule); a negative test result is colorless to pale pink.
anaerobic triphenyl tetrazolium reduction
A hydrolase assay for the presence of lipase activity in a microorganism. Lipase enzymes split longer-chained esters into an acid and an alcohol via a hydrolysis reaction.
An assay for the production of hydrogen sulfide in a microorganism from sodium thiosulfate under anaerobic conditions. After incubation, FeCl3 (ferric chloride) is added. If H2S is formed, it will react with the ferric chloride to form a black/brown ferrous sulfide precipitate. A positive test results in a black deposit (or thin line); a negative test results in a colorless or greyish color. Can also be performed using a strip of filter paper soaked in saturated lead acetate and dried. When suspended above the medium in the presence of hydrogen sulfide the paper will turn black.
organic molecular entity fermentation assay
decolorizes the sulphonphthalein dyes brom cresol purple and phenol red
Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be fermented by a microorganism. For fermentation of substrates in the presence of BROMCRESOL PURPLE (bromocresol purple), acidification of the medium results in a color change of the indicator to yellow or yellow-green. Absence of fermentation results in a neutral pH, or purple color. A positive reaction is purple; a negative reaction is yellow or yellow-green. ---------------------------------- For fermentation of substrates in the presence of PHENOL RED acidification of the medium results in a color change of the indicator to yellow. Absence of fermentation results in a neutral pH, or red color. A positive reaction is yellow; a negative reaction is red. ----------------------------------- For fermentation of substrates in the presence of BROMOTHYMOL BLUE, acidification of the medium results in a color change of the indicator to yellow or green. Absence of fermentation results in a neutral pH, or blue color. A positive reaction is green to yellow; a negative reaction is blue. ----------------------------------
Assays for the ability of a microorganism to ferment D-glucose.
Assays for the ability of a microorganism to ferment D-mannitol.
Assays for the ability of a microorganism to ferment beta-lactose.
Assays for the ability of a microorganism to ferment sucrose.
Assays for the ability of a microorganism to ferment beta-maltose.
Assays for the ability of a microorganism to ferment salicin.
Assays for the ability of a microorganism to ferment D-xylose.
Assays for the ability of a microorganism to ferment L-arabinose.
Assays for the ability of a microorganism to ferment glycerol.
Assays for the ability of a microorganism to ferment beta-cellobiose.
Assays for the ability of a microorganism to ferment D-mannose.
Assays for the ability of a microorganism to ferment melezitose.
Assays for the ability of a microorganism to ferment D-raffinose (raffinose).
Assays for the ability of a microorganism to ferment D-glucitol.
Assays for the ability of a microorganism to ferment L-rhamnose.
alpha,alpha-trehalose fermentation assay
Assays for the ability of a microorganism to ferment alpha,alpha-trehalose.
An assay for the hydrolysis of esculin in a microorganism. Esculin is a coumarin glycoside. Many species of microorganisms can hydrolyze esculin into glucose and aesculetin (6,7-dihydroxycoumerin). In the presence of ferric iron, aesculetin reacts to form a dark brown to black phenolic iron compound. A positive result generates a dark brown/black precipitate; a negative result generates no precipitate (yellow color). Because esculin is fluorescent, under uv illumination, a positive result generates no fluorescence; a negative result generates fluorescence. Ferric iron may be reduced to hydrogen sulfide (resulting in a dark brown to black precipitate), resulting in a false positive reaction. Sulfide will be produced only under anaerobic conditions, so the precipitate will only be seen at the bottom of the cupule, away from oxygen. Confirmation of the test results should therefore be made using uv illumination.
Assays for the ability of a microorganism to ferment erythritol.
An assay for the activity of beta-galactosidase in a microorganism. An assay for beta-galactosidase activity using para-nitrophenyl-beta-D-galactopyranoside; 4-nitrophenyl-beta-D-galactopyranoside; PNPG). Beta-galactosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless. PNPG has been found in some strains to have a higher hydrolysis rate than ONPG, and beta-galactosidase has a higher substrate preference for PNPG. Lee E-G, Kim S, Oh D-B, Lee SY & Kwon O. 2012. Distinct roles of beta-galactosidase paralogues of the rumen bacterium Mannheimia succiniciproducens. J Bacterol 194(2):426-436.
Assays for the ability of a microorganism to assimilate D-glucose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-arabinose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-mannose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-mannitol as a sole source of carbon and energy.
N-acetyl-beta-D-glucosamine assimilation assay
N-acetyl-beta-D-glucosamine assimilation
Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-glucosamine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate beta-maltose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-gluconate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate decanoate (decanoic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate adipate (adipic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate (S)-malate(2-) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate citrate (citric acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate phenylacetate (phenylacetic acid) as a sole source of carbon and energy.
organic molecular entity assimilation assay
Organic carbon metabolism assay where an organic molecular entity is tested to determine if it can be assimilated or oxidized by a microorganism, using it both as a carbon source and as a source of energy. Assimilation of the substrate in the presence of a single organic carbon compound results in turbidity due to growth, under oxic conditions. Oxidation means that the compound is oxidized (which could be followed by the oxidation of a terminal electron acceptor dye, such as resazurin or tetrazolium-derivative). Note: this definition does not include fermentation. A positive result is the presence of turbidity (opaque); a negative result is transparent. Or a positive result is the presence of a change in color of a redox dye; a negative result is no change in color.
Assays for the ability of a microorganism to ferment D-arabinose.
Assays for the ability of a microorganism to ferment D-ribose.
Assays for the ability of a microorganism to ferment L-xylose.
Assays for the ability of a microorganism to ferment D-ribitol.
methyl beta-D-xylopyranoside fermentation assay
Assays for the ability of a microorganism to ferment methyl-beta-D-xylopyranoside.
methyl beta-D-xylopyranoside fermentation
methyl beta-D-xylopyranoside acidification
Assays for the ability of a microorganism to ferment D-galactose.
Assays for the ability of a microorganism to ferment D-fructose.
Assays for the ability of a microorganism to ferment L-sorbose.
Assays for the ability of a microorganism to ferment galactitol.
Assays for the ability of a microorganism to ferment inositol.
methyl alpha-D-mannoside fermentation assay
methyl alpha-D-mannopyranoside acidification
methyl alpha-D-mannopyranoside fermentation
Assays for the ability of a microorganism to ferment methyl-alpha-D-mannoside.
methyl alpha-D-glucopyranoside fermentation assay
methyl alpha-D-glucopyranoside fermentation
Assays for the ability of a microorganism to ferment methyl-alpha-D-glucopyranoside.
methyl alpha-D-glucopyranoside acidification
methyl-a-D-glucopyranoside acidification
N-acetyl-beta-D-glucosamine fermentation assay
N-acetyl-beta-D-glucosamine acidification
N-acetyl-beta-D-glucosamine fermentation
Assays for the ability of a microorganism to ferment N-acetyl-beta-D-glucosamine.
Assays for the ability of a microorganism to ferment D-amygdalin.
hydroquinone O-beta-D-glucopyranoside fermentation assay
Assays for the ability of a microorganism to ferment hydroquinone O-beta-D-glucopyranoside.
Assays for the ability of a microorganism to ferment esculin.
Assays for the ability of a microorganism to ferment D-melibiose (beta-melibiose).
BMC Plant Biol. 2009; 9: 14. Published online Jan 28, 2009. doi: 10.1186/1471-2229-9-14 PMCID: PMC2660907 Construction of 12 EST libraries and characterization of a 12,226 EST dataset for chicory (Cichorium intybus) root, leaves and nodules in the context of carbohydrate metabolism investigation Nicolas Dauchot, Dominique Mingeot, Bénédicte Purnelle, Céline Muys, Bernard Watillon, Marc Boutry, and Pierre Van Cutsem.
Assays for the ability of a microorganism to ferment inulose (inulobiose). Inulin is a fructooligosaccharide polymer, found in some plants, which often has a terminal glucose. Glucose-free inulin is inulose. Think it is more likely that it is a mistaken representation for inulobiose.
Assays for the ability of a microorganism to ferment starch.
Assays for the ability of a microorganism to ferment glycogen.
Assays for the ability of a microorganism to ferment xylitol.
Assays for the ability of a microorganism to ferment gentiobiose.
Assays for the ability of a microorganism to ferment D-turanose.
Assays for the ability of a microorganism to ferment D-lyxose.
Assays for the ability of a microorganism to ferment D-tagatose.
Assays for the ability of a microorganism to ferment D-fucose.
Assays for the ability of a microorganism to ferment L-fucose.
Assays for the ability of a microorganism to ferment D-arabinitol.
Assays for the ability of a microorganism to ferment L-arabinitol.
Assays for the ability of a microorganism to ferment gluconate (gluconic acid).
2-dehydro-D-gluconic acid fermentation assay
Assays for the ability of a microorganism to ferment 2-ketogluconate (2-dehydro-D-gluconate; 2-dehydro-D-gluconic acid).
5-dehydro-D-gluconic acid fermentation assay
Assays for the ability of a microorganism to ferment 5-dehydro-D-gluconate (5-dehydro-D-gluconic acid).
2-dehydro-D-gluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-dehydro-D-gluconate (2-dehydro-D-gluconic acid) as a sole source of carbon and energy.
3-hydroxybutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-hydroxybutyrate (3-hydroxybutyric acid) as a sole source of carbon and energy.
5-dehydro-D-gluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate 5-dehydro-D-gluconate (5-dehydro-D-gluconic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate acetate (acetic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-alanine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-fucose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycogen as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-histidine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate inositol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate itaconate (itaconic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate (S)-lactate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate beta-melibiose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate malonate (malonic acid) as a sole source of carbon and energy.
3-hydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-hydroxybenzoate (3-hydroxybenzoic acid) as a sole source of carbon and energy.
4-hydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-hydroxybenzoate (4-hydroxybenzoic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-proline as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate propionate (propionic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-rhamnose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-ribose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate sucrose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate salicin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-serine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-glucitol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate suberate (suberic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate valerate (valeric acid) as a sole source of carbon and energy.
An assay for the presence of an enzyme or enzymatic capability of a microorganism.
An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
6-phospho-beta-galactosidase assay with pNP
An assay for the activity of 6-phospho-beta-galactosidase in a microorganism. Uses the substrate 4-nitrophenyl beta-D-galactopyranoside-6-phosphate. 6-phospho-beta-galactosidase (beta-galactosidase 6-phosphate) will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An assay for the activity of alpha-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-D-glucopyranoside. Alpha-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-glucopyranoside. Beta-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
Morphologically differentiated filament part, where the sheath has distinctive features.
An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
N-acetyl-beta-glucosaminidase assay with pNP
An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-beta-D-glucosaminide. N-acetyl-beta-glucosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An antibiotic resistance assay for the ability of a microorganism to utilize sugars in the presence of novobiocin.
The purpose of this test is to determine if a microbial isolate is capable of metabolizing glutamic acid under anaerobic conditions. When glutamic acid is metabolized, the pH of the medium decreases due to the accumulation of H+ and organic acids (e.g. glutamate, succinate). This turns color of the pH indicator (e.g. 4-nitrophenol) to yellow. A positive test yields a yellow color; a negative test is colorless. Glutamate decarboxylase carries out the following reaction: L-glutamate <=> 4-aminobutanoate (gamma-aminobutyrate) + CO2
An assay for the activity of alpha-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-L-fucopyranoside. Alpha-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An L-arginine arylamidase assay that uses the substrate L-arginine-2-naphthylamide (L-arginine-beta-naphthylamide) at pH 7.5. Arginine arylamidase activity (which could be from arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Uses the substrate L-proline-2-naphthylamide at pH 7.5. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for leucyl glycine arylamidase activity. Uses the substrate L-leucyl-L-glycine-2-naphthylamide at pH 7.5. Leucyl glycine arylamidase activity (which could be from leucyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-phenylalanine-2-naphthylamide at pH 7.5. Phenylalanine arylamidase activity (which could be from phenoylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
L-phenylalanine 7-amido-4-methylcoumarin
An alpha-amino acid arylamidase assay that uses the substrate pyroglutamic acid-2-naphthylamide (pyroglutamic acid-beta-naphthylamide) at pH 7.5. Pyroglutamic acid arylamidase activity (which could be from pyroglutamic acid aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
L-Pyroglutamic acid 7-amido-4-methylcoumarin
An alpha-amino acid arylamidase assay that uses the substrate L-tyrosine-2-naphthylamide at pH 7.5. Tyrosine arylamidase activity (which could be from tyrosine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate glycine-2-naphthylamide at pH 7.5. Glycine arylamidase activity (which could be from glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-histidine-2-naphthylamide at pH 7.5. Histidine arylamidase activity (which could be from histidine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
glutamyl glutamic acid arylamidase assay
L-glutamyl-L-glutamic acid-2-naphthylamide
An amino acid arylamidase assay that assays for Glutamyl glutamic arylamidase activity. Uses the substrate L-glutamyl-L-glutamic acid-2-naphthylamide at pH 7.5. Glutamyl glutamic arylamidase activity (which could be from glutamyl glutamic aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-serine-2-naphthylamide at pH 7.5. Serine arylamidase activity (which could be from serine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An assay for alanyl alanine arylamidase activity. Uses the substrate L-alanyl-L-alanine-2-naphthylamide at pH 7.5. Alanyl alanine arylamidase activity (which could be from alanyl alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
A carbohydrate hydrolysis assay for the activity of beta-galactosidase in a microorganism using various chromogenic substrates.
An assay for the activity of beta-glucosidase in a microorganism using various chromogenic substrates. Beta-glucosidase hydrolyses oligosaccharides (such as cellobiose) into monosaccharides.
A carbohydrate hydrolysis assay for the activity of beta-glucuronidase in a microorganism using various chromogenic substrates.
A carbohydrate hydrolysis assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism using various chromogenic substrates.
API® 20 Strep; Identification system for Streptococcaceae and related organisms; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
An assay for the hydrolysis of hippuric acid (benzoylaminoethanoic acid; N-benzoylglycine) in a microorganism. The test uses ninhydrin as a color indicator. Hippuric acid hydrolysis releases glycine, which reacts with ninhydrin (colorless) to form a purple color. A positive test result is dark blue/violet; a negative test result is colorless/pale blue/blueish-grey.
API® NH; System for the identification of Neisseria and Haemophilus; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Uses the substrate 5-bromo-3-indoxyl-caprate (5-bromo-3-indolyl decanoate; blue-caprate). Lipases that can hydrolyze C10 compounds will cleave the substrate, producing 5-bromo-3-hydroxyindole. The latter will spontaneously dimerize and oxidize to 5,5'-dibromo-indigo, an intensely blue insoluble product. A postive reaction is blue with a precipitate; a negative reaction is colorless to pale grey.
Kiernan JA. 2007. Indigogenic substrates for detection and localization of enzymes. Biotechnic & Histochemistry 82(2):73-103.
An antibiotic resistance assay for the activity/presence of penicillinase using potassium benzylpenicillin (penicillin G) and starch iodide. Penicillin hydrolysis results in the decolorization of starch iodide due to the reduction of iodine. A positive reaction (penicillinase presence) is yellow, yellow-green, or yellow-blue. A negative reaction is blue. Perret CJ. 1954. Iodometric assay of penicillinase. Nature 174:1012-1013.
gamma glutamyl transferase assay using methoxy-NA
An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using gamma-glutamyl-4-methoxy-beta-naphthylamide. Gamma glutamyl transferase activity reacts with the substrate releasing beta-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive test is orange/dark orange; a negative test is colorless, yellow to pale orange (may be yellow orange if the organism is positive for alkaline phosphatase).
Assays for the ability of a microorganism to utilize malonate.
API® Rapid ID 20 E – System for the identification of Enterobacteriaceae in 4 hours; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Measures the presence of para-phenylalanine deaminase activity using the substrate 4-nitrophenylalanine. Para-phenylalanine deaminase activity cleaves the compound releasing ammonia and 3-(4-nitrophenyl)propanoic acid (yellow to brown). A positive reaction is yellow to brown; a negative reaction is colorless.
API® Coryne; Identification system for coryneform bacteria ; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
An enzymatic assay which tests for the presence of pyrazinamidase activity using the substrate pyrazinecarboxamide. Pyrazinamidase hydrolyzes the substrate pyrazinamide (pyrazinecarboxamide) to form pyrazinoic acid (pyrazine-2-carboxylic acid). In the presence of ferrous ammonium sulfate a complex forms witht he product to form a "rusty pink color complex". A positive test is brown orange; a negative test is colorless/very pale brown/very pale orange. Pyrazinamidase catalyzes the following reaction: Pyrazinamide + H2O <=> pyrazinoic acid (pyrazine-2-carboxylic acid) + NH3.
Revathi G & Talwar V. 1995. A biochemical test based on pyrazinamidase activity for rapid differentiation of Corynebacteria. Indian J Clin Biochem 10(1):39-41.
An assay for the activity of alpha-glucosidase in a microorganism using various chromogenic substrates. Alpha glucosidase acts on 1,4-alpha bonds in polymers of glucose.
An assay for the activity of alpha-glucosidase in a microorganism. Uses the substrate 2-naphthyl alpha-D-glucopyranoside. Alpha-glucosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless, beige-pale purple, pale green.
API® Campy; Identification system for Campylobacter; http://www.biomerieux-usa.com; API is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Test for C2 esterase activity in a microorganism using the substrate 5-bromo-4-chloro-3-indoxyl-acetate. Esterase that can hydrolyze C2 compounds will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole. The later will spontaneously dimerize and oxidize to 5,5'-dibromo-4,4'-dichloro-indigo, an intensely blue insoluble product. A postive reaction is blue/turquiose with a precipitate; a negative reaction is colorless to pale blue. Kiernan JA. 2007. Indigogenic substrates for detection and localization of enzymes. Biotechnic & Histochemistry 82(2):73-103.
An alpha-amino acid arylamidase assay that uses the substrate L-aspartate-2-naphthylamide (L-aspartic acid-beta-naphthylamide) at pH 7.5. Aspartate arylamidase activity (which could be from aspartate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Assays for the ability of a microorganism to assimilate succinate (succinic acid) as a sole source of carbon and energy.
An assay for the ability of a microorganism to grow or utilize sugars in the presence of an antibiotic (an antimicrobial compound).
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of nalidixic acid.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of (sodium) cefazolin.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of erythromycin.
A carbohydrate hydrolysis assay for the activity of alpha-galactosidase in a microorganism using various chromogenic substrates.
alpha-galactosidase assay with BrNaphthol
An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless.
N-acetyl-beta-glucosaminidase assay with oNP
An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 2-nitrophenyl-N-acetyl-beta-D-glucosaminide (o-nitrophenyl-N-acetyl-beta-D-glucosaminide). N-acetyl-beta-glucosaminidase will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An enzymatic assay which tests for the ability of a microorganism to metabolize (for example, by hydrolysis or decarboxylation) or amino acid-containing substrates.
Amino acid metabolism assay which tests for the ability of a microorganism to hydrolyze amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety. Arylamidases are classes of enzymes with the ability to hydrolyze amino acid-beta-naphtylamide substrates. Sheahan JP, Eitenmiller RR, & Carpenter JA. 1975. Arylamidase activity of Salmonella species. Appl Microbiol 29(6):726-728.
Amino acid metabolism assay which tests for the ability of a microorganism to grow/metabolize using an amino acid as a source of energy.
Wikipedia: Electromagnetic radiation Electromagnetic radiation (EM radiation or EMR) is a form of radiant energy released by certain electromagnetic processes. Visible light is one type of electromagnetic radiation, other familiar forms are invisible electromagnetic radiations such as X-rays and radio waves.
Radiant energy released by some electromagnetic processes, including visible light.
Amino acid metabolism assay which tests for the ability of a microorganim to gain energy by the deamination of an amino acid.
An enzymatic assay which tests for the ability of a microorganism to hydrolyze carbohydrates or carbohydrate derivatives.
An enzymatic assay which tests for the ability of a microorganism to hydrolyze proteins or oligopeptides.
An assay for the ability of a microorganism to oxidize or reduce specific chemical compounds.
An organic-rich, solid microbiological culture medium that contains tryptone, yeast extract, skim milk powder, and glucose. Used for the cultivation of microorganisms in dairy products.
From: Milk Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Milk Agar is recommended by the British Standards Institute1 for the enumeration of microorganisms in liquid milk, ice cream, dried milk and whey. Principles of Procedure Peptone and yeast extract provide essential nutrients while skim milk powder is a source of casein. Dextrose is the carbon energy source. Agar is the solidifying agent. Proteolytic bacteria will be surrounded by a clear zone from the conversion of casein into soluble nitrogenous compounds.1 Formula Difco™ Milk Agar Approximate Formula* Per Liter Tryptone...................................................................... 5.0 g Yeast Extract................................................................ 2.5 g Dextrose...................................................................... 1.0 g Skim Milk Powder (antibiotic free)................................ 1.0 g Agar.......................................................................... 12.5 g *Adjusted and/or supplemented as required to meet performance criteria. pH 6.9 ± 0.1
An alpha-amino acid arylamidase assay that uses the substrate alanine-2-naphthylamide (L-alanine-beta-naphthylamide) at pH 7.5. Alanine arylamidase activity (which could be from alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using L-alanine-p-nitroanilide. Alanine arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-asparagine-2-naphthylamide (L-asparaginyl-beta-naphthylamide) at pH 7.5. Asparagine arylamidase activity (which could be from asparagine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Uses the substrate L-alpha-glutamate-2-naphthylamide (L-alpha-glutamyl-beta-naphthylamide) at pH 7.5. Alpha-glutamate arylamidase activity (which could be from alpha-glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
gamma-glutamyl arylamidase assay using NA
Uses the substrate gamma-glutamate-2-naphthylamide (gamma-glutamyl-beta-naphthylamide) at pH 7.5. Gamma-glutamate arylamidase activity (which could be from gamma-glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-hydroxyproline-2-naphthylamide (L-hydroxyprolyl-beta-naphthylamide) at pH 7.5. Hydroxyproline arylamidase activity (which could be from hydroxyproline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-lysine-2-naphthylamide (L-lysyl-beta-naphthylamide) at pH 7.5. Lysine arylamidase activity (which could be from lysine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Uses the substrate L-methionine-2-naphthylamide (L-methionyl-beta-naphthylamide) at pH 7.5. Methionine arylamidase activity (which could be from methionine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Uses the substrate L-leucine-4-methoxy-2-naphthylamide (4-methoxy-leucyl-beta-naphthylamide, L-leucine 4-methoxy-beta-naphthylamide) at pH 7.5. Methoxy leucine arylamidase activity (which could be from methoxy leucine aminopeptidase, aminopeptidase M, or leucine aminopeptidase, as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-ornithine-2-naphthylamide (L-ornithyl-beta-naphthylamide) at pH 7.5. Ornithine arylamidase activity (which could be from ornithine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-threonine-2-naphthylamide (L-threonyl-beta-naphthylamide) at pH 7.5. Threonine arylamidase activity (which could be from threonine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-tryptophan-2-naphthylamide (L-tryptophyl-beta-naphthylamide) at pH 7.5. Tryptophan arylamidase activity (which could be from tryptophan aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-glutamine-2-naphthylamide (L-glutaminyl-beta-naphthylamide) at pH 7.5. Glutamine arylamidase activity (which could be from glutamine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-isoleucyl-2-naphthylamide (L-isoleucine-beta-naphthylamide) at pH 7.5. Isoleucine arylamidase activity (which could be from isoleucine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for seryl tyrosine arylamidase activity. Uses the substrate L-seryl-L-tyrosine-2-naphthylamide at pH 7.5. Seryl tyrosine arylamidase activity (which could be from seryl tyrosine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for glycyl proline arylamidase activity. Uses the substrate glycyl-L-proline-2-naphthylamide at pH 7.5. Glycyl proline arylamidase activity (which could be from glycyl proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for glycyl phenylalanine arylamidase activity. Uses the substrate glycyl-L-phenylalanine-2-naphthylamide at pH 7.5. Glycyl phenylalanine arylamidase activity (which could be from glycyl phenylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate DL-methionine-2-naphthylamide (DL-methionyl-beta-naphthylamide) at pH 7.5. DL-Methionine arylamidase activity (which could be from methionine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
glutaryl phenylalanine arylamidase assay
An amino acid arylamidase assay that assays for glutaryl phenylalanine arylamidase activity. Uses the substrate glutaryl-L-phenylalanine-2-naphthylamide at pH 7.5. Glutaryl phenylalanine arylamidase activity (which could be from glutaryl phenylalanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
N-carbobenzoyl-glycyl-glycyl-L-arginine peptidase assay
An amino acid arylamidase assay that assays for the presence of N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide peptidase in a microorganism. Uses N-carbobenzoyl-glycyl-glycyl-arginine-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
N-benzoyl-L-valyl-glycyl-L-arginine-4-methoxy peptidase assay
An amino acid arylamidase assay that assays for the presence of N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide peptidase in a microorganism. Uses N-benzoyl-L-valyl-glycyl-4-methoxy-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An assay for the ability of a microorganism to hydrolyze proteins.
An assay for the activity of alpha-fucosidase in a microorganism using various chromogenic substrates which carries out the following reaction: alpha-L-fucoside + H2O <=> L-fucose + an alcohol
An amino acid arylamidase assay that assays for the presence of N-benzoyl-L-leucine-beta-naphthylamide peptidase in a microorganism. Uses N-benzoyl-L-leucine-beta-naphthylamide as a substrate. Hydrolysis of the substrate releases 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An assay for the activity of alpha-fucosidase in a microorganism. Uses the substrate 2-naphthyl-alpha-L-fucopyranoside. Alpha-fucosidase will cleave the substrate, producing 2-napthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is purple in color. A positive result is purple; a negative result is colorless.
N-acetyl-alpha-D-galactosaminidase assay
A carbohydrate hydrolysis assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism using various chromogenic substrates.
N-acetyl-alpha-D-galactosaminidase assay with pNP
An assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-alpha-D-galactosaminide (p-nitrophenyl-N-acetyl-alpha-D-galactosaminide). N-acetyl-alpha-galactosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
N-acetyl-alpha-D-galactosaminidase assay with oNP
An assay for the activity of N-acetyl-alpha-galactosaminidase in a microorganism. Uses the substrate 2-nitrophenyl-N-acetyl-alpha-D-galactosaminide (o-nitrophenyl-N-acetyl-alpha-D-galactosaminide). N-acetyl-alpha-galactosaminidase will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
A carbohydrate hydrolysis assay for the activity of beta-xylosidase in a microorganism using the substrate 4-nitrophenyl-beta-D-xylopyranoside. Beta-xylosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
A carbohydrate hydrolysis assay for the activity of alpha-xylosidase in a microorganism using the substrate 4-nitrophenyl-alpha-D-xylopyranoside. Alpha-xylosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An assay for the activity of beta-L-fucosidase in a microorganism using various chromogenic substrates.
An assay for the activity of beta-L-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-L-fucopyranoside. Beta-L-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
Acid phosphatase catalyzes the following reaction (under acidic pH conditions): A phosphate monoester + H2O <=> an alcohol + phosphate
An assay for the activity of beta-fucosidase in a microorganism using various chromogenic substrates.
An assay for the activity of beta-D-fucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-fucopyranoside. Beta-D-fucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
Uses the substrate bis-para-nitrophenyl-phosphate (para-nitrophenylphosphate, 4-nitrophenyl phosphate). Under acidic conditions, acid phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An alkaline phosphatase catalyzes the following reaction (under alkaline pH conditions): A phosphate monoester + H2O <=> an alcohol + phosphate
Alkaline phosphatase assay that uses the substrate bis-para-nitrophenyl-phosphate (para-nitrophenylphosphate, 4-nitrophenyl phosphate). Under alkaline conditions, alkaline phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An enzymatic assay which tests for the presence of phosphodiesterase - an enzyme that hydrolyzes a phosphodiester bonds (two ester bonds between a phosphate group and two carbohydrates, i.e. sugar-phosphate-sugar).
Phosphonate hydrolase is an esterase that hydrolyzes a phosphonate compound (an organophosphorus compound with multiple alklyl or aryl groups). p-nitrophenyl phenyl phosphonate is a substrate (NPPP) for phosphonate hydrolase enzymes. Hydrolysis of this compound releases 4-nitrophenol (p-nitrophenol), which is yellow. A positive result is yellow; a negative result is colorless.
beta-glucosidase assay with pNP-lactoside
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-nitrophenyl-beta-D-lactopyranoside (lactopyranoside is a disaccharide comprised of lactose, or gal-glu). Beta-glucosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
As assay for the activity of phospholipase C (phosphorylcholine hydrolase); an enzyme that catalyzes the hydrolysis of the glycerophosphate bond of phospholipids. Uses the substrate bis-p-nitrophenyl-phosphoryl-choline. Phospholipase C will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
Test for the presence of arylsulfatase (sulfatase) in a microorganism using the chromogenic substrate 4-nitrocatechol sulfate (pNCS, 2-hydroxy-5-nitrophenyl sulfate). The substrate is cleaved (via hydrolysis reaction) to form p-nitrocatechol + sulfate. p-nitrocatechol (4-nitrocatechol), under alkaline conditions, is red. A positive test is red; a negative test is yellow.
Test for the presence of arylsulfatase (sulfatase) in a microorganism using a chromogenic substrate that contains an aryl group.
An enzymatic assay which tests for the presence of sulfatase in a microorganism using a chromogenic substrate. Sulfatases (EC 3.1.6) are esterases that catalyze the hydrolysis of sulfate esters.
A carbohydrate hydrolysis assay for the activity of alpha-glucosidase or beta-glucosidase in a microorganism using various chromogenic substrates.
A carbohydrate hydrolysis assay for the activity of alpha-mannosidase in a microorganism using various chromogenic substrates.
An assay for the activity of amylase in a microorganism using various chromogenic substrates.
An enzymatic assay which tests for the presence of DNase activity.
fructose-1,6-bisphosphate aldolase assay
A carbohydrate hydrolysis assay for the activity of fructose-1,6-bisphosphate aldolase (aldolase) using various chromogenic substrates. Catalyzes the reaction: fructose 1,6-bisphosphate <=> dihydroxyacetone phosphate (DHAP) + glyceraldehyde-3-phosphate (GAP)
organic molecular entity metabolic assay
An assay for the assimilation, fermentation, oxidation, or hydrolysis of an organic molecular entity by a microorganism.
An enzymatic assay which tests for the preence of uridine phosphorylase activity in a microorganism. Uridine phosphorylase catalyzes the reaction: uridine + phosphate <=> uracil + alpha-D-ribose-1-phosphate
A hydrolase assay for the presence of esterase activity (carboyxlic ester hydrolase activity) in a microorganism. Esterase enzymes split short-chained esters into an acid and an alcohol via a hydrolysis reaction.
A carbohydrate hydrolysis assay for the presence of 6-phosphogluconate dehydrogenase (EC: 1.1.1.43) in a microorganism. 6-phosphogluconate dehydrogenase is an enzyme in the pentose phosphate pathway and catalyzes the reaction: 6-phospho-D-gluconate + NAD(P) <=> 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+
Assays for the presence of maltase in a microorganism. Maltase is an alpha-glucosidase that hydrolyzes the alpha,1-4 bond in maltose (a disaccharide comprised of glucose monomers).
A carbohydrate hydrolysis assay for the presence of agarase activity in a microorganism. Agarase (4-glycanohydrolase) hydrolizes alpha or beta linkages in agarose, produing oligosaccharides. Agar is a purified form of the polysaccharide agarose (a polymer of D-galactose and 3,6-anhydro-L-galactopyranose, linked by alpha 1->3 and beta 1-> 4 bonds).
An amino acid arylamidase assay that uses the substrate beta-alanine-2-naphthylamide (beta-alanine beta-naphthylamide) at pH 7.5. Beta-alanine arylamidase activity (which could be from beta-alanine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using beta-alanine-p-nitroanilide. Beta-alanine arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
zzzz note: nitroanilide is not naphthylamide
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Gram negative bacterial identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
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Neisseria, Haemophilus and other fastidious Gram negative bacteria identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Anaerobic bacteria and coryneform bacteria identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
Assays for the ability of a microorganism to assimilate erythritol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycerol as a sole source of carbon and energy.
hydroquinone O-beta-D-glucopyranoside assimilation assay
Assays for the ability of a microorganism to assimilate hydroquinone O-beta-D-glucopyranoside as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate amygdalin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-galactose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate gentiobiose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate alpha-lactose as a sole source of carbon and energy.
methyl alpha-D-glucopyranoside assimilation assay
methyl-alpha-D-glucopyranoside assimilation
Assays for the ability of a microorganism to assimilate methyl-alpha-D-glucopyranoside as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate beta-cellobiose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate raffinose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate melezitose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-sorbose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate cis-aconitate (cis-aconitic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate xylitol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate turanose as a sole source of carbon and energy.
alpha,alpha-trehalose assimilation assay
Assays for the ability of a microorganism to assimilate alpha,alpha-trehalose as a sole source of carbon and energy.
Assays for the ability of a microorganism to ferment 1-phenylethylamine.
alpha-D-galacturonic acid assimilation assay
Assays for the ability of a microorganism to assimilate D-galacturonate (D-galacturonic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-glutamate (L-glutamic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-xylose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-glucuronate (D-glucuronic acid) as a sole source of carbon and energy.
The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of CO2 and organic amines (e.g. agmatine, putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine decarboxylase catalyzes the following reaction: L-arginine <=> agmatine + CO2
glutamyl glycyl arginine arylamidase assay
An amino acid arylamidase assay that assays for glutamyl glycyl arginine arylamidase activity. Uses the substrate L-glutamyl-glycyl-L-arginine-2-naphthylamide at pH 7.5. Glutamyl glycyl arginine arylamidase activity (which could be from glutamyl glycyl arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that uses the substrate L-glutamate-2-naphthylamide (L-glutamyl-beta-naphthylamide) at pH 7.5. Glutamate arylamidase activity (which could be from glutamate aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless. Test is also performed using L-glutamate-p-nitroanilide. Glutamate arylamidase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
A multicellular prokaryotic morphological quality where cells so that their long axes are stacked parallel to one another (like in a fence).
A carbohydrate hydrolysis assay for the activity of N-acetyl-beta-galactosaminidase in a microorganism using various chromogenic substrates.
A carbohydrate hydrolysis assay for the activity of beta-lactamase in a microorganism using various chromogenic substrates. Beta-lactams have at the core of their structure cyclic amides comprised of four-membered ring where one of the atoms is N and the neighboring carbon atom is attached to oxygen via a carbonyl group. Beta lactamase hydrolyzes the beta-lactam ring, resulting in the resistance of the microorganism to beta-lactam antibiotics.
A carbohydrate hydrolysis assay for the activity of xylanase in a microorganism using various chromogenic substrates. Xylanase catalyzes the hydrolysis of beta(1->4) bonds in beta-1,4-xylan polysaccharides (such as hemicellulose), producing xylose (a 5 carbon sugar).
N-acetyl-beta-galactosaminidase assay with pNP
An assay for the activity of N-acetyl-beta-galactosaminidase in a microorganism. Uses the substrate 4-nitrophenyl-N-acetyl-beta-D-galactosaminide (p-nitrophenyl-N-acetyl-beta-D-galactosaminide). N-acetyl-beta-galactosaminidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
Assays for the ability of a microorganism to assimilate galactitol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate myo-inositol as a sole source of carbon and energy.
commercial suite of microbiological diagnostic tests
6-O-alpha-D-glucopyranosyl-D-fructofuranose assimilation assay
Assays for the ability of a microorganism to assimilate 6-O-alpha-D-glucopyranosyl-D-fructofuranose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate pyruvate (pyruvic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to ferment (R)-malate or (R)-malic acid.
Assays for the ability of a microorganism to ferment maltotriose.
Assays for the ability of a microorganism to assimilate maltotriose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-glutamine as a sole source of carbon, nitrogen, and energy.
An enzymatic assay which tests for the ability of a microorganism to break down lecithin (a generic class fo fatty substances in animal and plant tissues, including phosphrylcholine, fatty acids, glycolipids, triglycerides and phospholipids). Measured by observing a precipitate (an opaque halo) around a microbial colony on egg yolk agar.
Assays for the ability of a microorganism to ferment xylan.
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indolyl-beta-glucoside. beta-Glucosidase activity will cleave the substrate, producing 5-bromo-4-chloro-3-indoxyl.
An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-beta-glucuronide. Beta-glucuronidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
An assay for the activity of beta-galactosidase in a microorganism. An assay for beta-galactosidase activity using X-gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside). Beta-galactosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-alpha-galactoside. Alpha-galactosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
N-acetyl-beta-glucosaminidase assay with indoxyl
An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 5-bromo-4-chloro-3-indoxyl-beta-N-acetyl-glucosamine. N-acetyl-beta-glucosaminidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
An assay for the activity of alpha-mannosidase in a microorganism. An assay for alpha-mannosidase activity using 5-bromo-4-chloro-3-indoxyl-alpha-mannoside. Alpha-mannosidase will cleave the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
Assays for the ability of a microorganism to assimilate lactate (lactic acid, 2-hydroxypropanoic acid, DL-lactate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-fructose as a sole source of carbon and energy.
Assays for the ability of a microorganism to ferment inulin.
A test or series of tests for enzymes in the denitrification pathway. Nitrate (NO3-) may be reduced to nitrite (NO2-) and then to nitrous oxide (N2O), nitric oxide (NO), nitrogen (N2), or ammonia.
Test for the presence of nitrite reductase in a microbial culture. Nitrite reacts with sulfanilic acid under acidic conditions (5N acetic acid) to produce (colorless) diazotized sulfanilic acid. This sulfanilic acid is then reacted to (colorless) alpha-naphthylamine (or N,N-dimethyl-alpha-naphthylamine) producing a red water soluble azo dye (p-sulfobenzene-azo alpha-naphthylamine). Presence of nitrite then results in a pink/red color (a negative test result).
Test for the presence of nitrite reductase in a microbial culture. Complete nitrate reduction to dinitrogen gas will result in the production of gas bubbles.
An assay for measuring the presence of low molecular weight thiols (such as glutatione) in liquid solutions, blood, or microbiological cultures. Uses Ellman's reagent (5-5'-dithiobis-(2-nitrobenzoic acid); DTNB) - a chromogenic agent containing a disulfide bond. The substrate reacts with small molecule thiols (e.g. glutathione) and reduce the disulfide bonds, releasing 3-thio-6-nitrobenzoate. At neutral or alkaline pH the product spontaneously ionizes, resulting in a yellow color. A positive result is yellow; a negative result is colorless.
An L-arabinofuranosidase assay for the activity of alpha-L-arabinofuranosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal alpha-L-arabinoside residues in alpha-L-arabinosides.
Dotson,S.B., Smith,C.E., Ling,C.S., Barry,G.F. and Kishore,G.M. (1996) Identification, characterization, and cloning of a phosphonate monoester hydrolase from Burkholderia caryophilli PG2982. J. Biol. Chem. 271 (42), 25754-25761.
Phosphonate hydrolase is an esterase that hydrolyzes a phosphonate compound (an organophosphorus compound with multiple alklyl or aryl groups). Assays for the presence of this enzyme using various chromogenic substrates.
Assays for the ability of a microorganism to ferment cyclodextrin (a family of cyclic oligosaccharides) that are intermediates in the breakdown of starch.
Assays for the ability of a microorganism to assimilate putrescine as a sole source of carbon and energy.
Assays for the ability of a microorganism to ferment pyruvate (pyruvic acid).
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of kanamycin.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of oleandomycin.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of polymixin B.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of O/129 (vibriostatic agent, 2,4-diamino-6,7-diisopropylpteridine).
An assay for the ability of an organism to ferment coumarate (coumaric acid).
phosphatidylinositol phospholipase C assay
As assay for the activity of phosphatidylinositol phospholipase C; an enzyme that catalyzes the hydrolysis of the phosphatidylinositol into inositol triphosphate and diacylglycerol. Assay is made using various chromogenic and fluorogenic substrates.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of bacitracin.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of optochin (ethylhydrocupreine).
methyl beta-D-glucopyranoside fermentation assay
Assays for the ability of a microorganism to ferment methyl-beta-D-glucopyranoside.
methyl-b-D-glucopyranoside acidification
methyl-beta-D-glucopyranoside fermentation
methyl-beta-D-glucopyranoside acidification
Assays for the ability of a microorganism to ferment pullulan (a polysaccharide polymer of maltotriose subunits).
Amino acid metabolism assay which tests for the presence of arginase (arginine amidinase; canavanase; L-arginase; arginine transamidinase; arginine argiamidase) in a microorganism. When arginine is metabolized, urea is produced. Urea reacts with the colorometric substrates (o-phthalaldehyde and N-(1-naphthyl)ethylenediamine) resulting in a colored pigment whose adsorption is measured at 430 nm. A positive test yields a red/orange color; a negative test is yellow. Arginase catalyzes the reaction: L-arginine + H2O <=> L-ornithine + urea Zawada RJX, Kwan P, Olszewski KL, Llinas M & Huang S-G. 2009. Quantitative determination of urea concentrations in cell culture medium. Biochem Cell Biol 87(3):541-544.
Bacillus identification; http://www.biomerieux-usa.com VITEK® is a registered trademark belonging to bioMerieux SA or one of its subsidiaries.
An assay for the ability of a microorganism to grow (i.e. undergo cell division) in microbiological culture medium that has various concentrations of sodium chloride (NaCl).
An assay for the ability to grow in the presence of 6.5% sodium chloride (NaCl).
Organic carbon metabolism assay where the ability of a microorganism to increase the pH of the medium as the result of the utlization/metabolism of organic acids (carboxylic acid anions) is assayed.
Wikipedia: Coagulase Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive.[1] [2] It is also produced by Yersinia pestis.[3] Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen, a plasma protein produced by the liver, to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. The fibrin clot may protect the bacterium from phagocytosis and isolate it from other defenses of the host.[4] It has been proposed that fibrin-coated staphylococci resist phagocytosis, making the bacteria more virulent. Bound coagulase is part of the larger family of MSCRAMM.
Catalysis of the hydrolysis of internal, alpha-peptide bonds in a polypeptide chain by a mechanism in which water acts as a nucleophile, where the polypeptide chain is prothrombin.
Assays for the ability of a microorganism to assimilate succinate (succinic acid) as a sole source of carbon. Assimilation of succinate results in an increase in pH (alkalinization) as a result of decarboxylation of succinate into propionate and CO2 via succinate decarboxylase. Involves the following enzymes: Succinate-CoA ligase (succinate + CoA + NTP = NDP+ Pi + succinyl-CoA) Methylmalonyl-CoA mutase (succinyl-CoA = methylmalonyl-CoA) Propionyl-CoA carboxylase (methylmalonyl-CoA + Pi + ADP = CO2 + ATP + propanoyl-CoA)
Macy JM, Ljungdahl LG, Gottschalk G. 1978. Pathway of succinate and propionate formation in Bacteroides fragilis. J Bact 134(1):84-91.
An assay for the presence of iron-phorphyrin compounds in a microorganism or cell culture. In the presence of hydrogen peroxide and iron-porphyrins, benzidine (4,4'-diaminobiphenyl) forms quinoidic rings (6 membered rings with double bonds) that impart a blue color. Benzidine and other aromatic amine dyes are no longer used because they are carcinogenic. Deibel RH & Evans JB. 1960. Modified benzidine test for the detection of cytochrome-containing respiratory systems in microorganisms. J Bacteroil 79(3):356-360.
A phenomenon in Gram staining where the ends of some rods or bacilli stain very little in the center, staining preferentially at the ends of the cells. Bipolar staining is common in enteric Gram-negative bacilli, and can make rods falsely appear to be diplococci.
An enzymatic assay which tests for the presence of chondroitin sulfatase (N-acetylgalactosamine-6-sulfatase) in a microorganism. Catalyzes the (hydrolysis) reaction of removing 6-sulfate groups from N-acetyl-D-galactosamine 6-sulfate subunits of the polymer chondroitin sulfate. Also catalyzes the reaction of removing sulfate from D-galactose 6-sulfate groups from keratan sulfate. [chondroitin]-6-O-sulfo-N-acetylgalactosamine + H2O = [chondroitin]-N-acetyl-galactosamine + SO42- + H+
A milk reactivity assay using to distinguish species of bacteria based on the increase or decrease of pH, protein degradation or denaturation, or lactose fermentation. Contains skim milk powder (which contains lactose and casein) and a pH indictor (litmus). Reactions: Blue color - indicates an alkaline pH (decreased acidity) Red color - indicates acidic pH due to fermentation of lactose (increased acidity) Clearing - indicates peptonization (dissolution of protein due to production of proteases; protein catabolic process) White precipitate (curd formation, clot formation) at the bottom of the tube - indicates milk coagulation (casein denaturation) Gas formation - indicates fermentation resulting in gas production This test yields a complex variation of results, and therefore is not very reliable. Best used as a confirmatory test, not as a diagnostic test.
Filament differentiation quality of heteropolar trichomes with respect to the shape of the trichome.
A milk reactivity assay which is used to determine if microorganisms produce acid when grown on milk. Milk (containing whey and casein) does not normally coagulate upon heating or boiling, however if acid (as a result of microbial fermentation) is present the casein will denature and curdle (i.e. coagulate).
Sommer HH, Hart EB. 1919. The heat coagulation of milk. J Biol Chem 40:137-151. From Summary and Conclusions: The main factor in the heat coagulation of fresh milk is the composition of the milk salts. Apparently casein requires a definite optimum calcium content for its maximum stability. the calcium content of casein is largley controlled by the magnesium, citrates, and phosphates present. Acid fermentation in milk lowers the coaguating point by changing the reaction and by lowering the citric acid content.
An assay for the ability of a microorganism to make collagenase. Collagenase ia a protease enzyme that hydrolyzes peptide bonds in collagen (a protein in connective tissues of animals). Are secreted enzymes by some pathogens.
An alpha-amino acid arylamidase assay that uses the substrate L-cysteine-2-naphthylamide at pH 7.5. Cysteine arylamidase activity (which could be from cysteine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Is an assay for the presence of phenylalanine deaminase activity in a microorganism. Uses phenylalanine agar which contains DL-phenylalanine and ferric chloride. If phenylpyruvic acid is produced, the ferric chloride will turn green. A posivite test is dark green; a negative test is straw in color. L-phenylalanine:2-oxoglutarate aminotransferase activity catalyzes the following: L-phenylalanine + 2-oxoglutarate = phenylpyruvate (phenylpyruvic acid) + L-glutamate
A carbohydrate hydrolysis assay for the ability of an organism to utilize/breakdown cellulose. Typically involves synergistic activity of many different types of endo- and exo-cellulases.
An assay for the ability of an organism to break down cellulose, or other beta-D-glucans. Cellulase (endo-1,4-beta-D-glucanose, beta-1,4-glucanse, beta-1,4-endoglucan hydrolase, celludextrinase, endoglucanase D) is a hydrolytic enzyme that catalyzes the breakdown of beta 1,4 linkages in cellulose, licenin, and cereal beta-D-glucans.
Test for the presence of exocellulase. Exocellulase is a hydrolytic enzyme that cleaves two or four units from the ends of exposed chains of beta-D-glucan (such as cellulose). Uses 4-methylumbelliferyl beta-D-glucopyranoside as a substrate. Results in the formation of tetra-, or di-saccharides and 4-methylumbelliferone.
A carbohydrate hydrolysis assay for the activity of alpha-fucosidase or beta-fucosidase in a microorganism using various chromogenic substrates.
An assay for the presence of an enzyme in a microorganism that can degrade fibrin ( a protein found in blood clots).
Wikipedia: Fibrinolysis Fibrinolysis is a process that prevents blood clots from growing and becoming problematic.[1] This process has two types: primary fibrinolysis and secondary fibrinolysis. The primary type is a normal body process, whereas secondary fibrinolysis is the breakdown of clots due to a medicine, a medical disorder, or some other cause.[1] In fibrinolysis, a fibrin clot, the product of coagulation, is broken down.[2] Its main enzyme plasmin cuts the fibrin mesh at various places, leading to the production of circulating fragments that are cleared by other proteases or by the kidney and liver.
An assay for the presence of an enzyme in a microorganism that can degrade fibrinogen (a protein which is required for blood clot formation).
Wikipedia: Fibrinogenolysis Primary fibrinogenolysis is a medical condition that appears with abnormal production of fibrinogen/fibrin degradation products (FDP), degradation of coagulation factors V, VIII, IX, XI and/or degradation of the fibrin present in any pre-existing localized thrombi and hemostatic clots.[1][2]
An amino acid arylamidase assay that assays for glutamyl glycine arylamidase activity. Uses the substrate L-glutamyl-glycine-2-naphthylamide at pH 7.5. Glutamyl glycine arylamidase activity (which could be from glutamyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for glycyl tryptophan arylamidase activity. Uses the substrate glycyl-L-tryptophan-2-naphthylamide at pH 7.5. Glycyl tryptophan arylamidase activity (which could be from glycyl tryptophan aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An amino acid arylamidase assay that assays for glycyl glycine arylamidase activity. Uses the substrate glycyl-glycine-2-naphthylamide at pH 7.5. Glycyl glycine arylamidase activity (which could be from glycyl glycine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
Assay to determine how a microorganism metabolizes glucose, and uses Hugh and Leifson's OF basal medium. Allows for the distinguishing of microorganisms that can ferment glucose anaerobically versus those that metabolize it aerobically (i.e. oxidatively). Organisms that can't metabolize the glucose are consitered to be nonsaccharolytic. The medium contains glucose, agar, with smaller amounts of potassium phosphate, peptone and sodium chloride. It also contains a pH indicator (such as bromthymol blue, which is yellow below pH 6.0 and blue above pH 7.6). The medium is inoculated by stabbing a culture with an inoculating needle half way to the bottom of two test tubes. In one tube, mineral oil is layered onto the agar to prevent oxygen from diffusing down into the culture medium. The culture is incubated. Fermentation of the carbohydrate in the anaerobic tube (and perhaps the aerobic tube) results in a yellow color due to the production of acid. Non-fermenting bacteria can metabolize the glucose aerobically. This is seen as the production of acid in the aerobic tube, but not in the anaerobic tube. Acid production in the aerobic tube should be near the top of the tube. Non-saccharolytic bacteria will result in a negative test - there will be no color change in either tube. In some cases a blue color may be seen in the top layer of the aerobic tube due to an increase in pH caused by the deamination of peptides in the peptone. Instead of glucose, other carbohydrates can be used.
An enzymatic assay which tests for the ability of a microorganism to produce hyaluronidase, and can therefore degrade (hydrolyze) hyaluronic acid (an anionic glycosaminoglycan, found in animal tissues). Assay is performed by adding an acidic albumin solution (pH 3.75 or 4.2). Undegraded hyaluronic acid will become turbid under these conditions, and the turbidity can be measured at 540 or 600 nm using an standard curve of hyaluronic acid that has been dissolved by boiling.
A microbiological diagnostic assay for the presence of flexirubin-type pigments. Upon flooding a colony with 20% KOH, a bathochromic shift (a shift in the absorbance of a compound) occurs resulting in the yellow (or orange) flexirubin pigment turning red/brown/purple. When flooded with an acidic solution, the color will revert back to the original color. Reichenbach, H. 1989. Order I: Cytophagales Leadbetter 1974. In: J. T. Staley, M. P. Bryant, N. Pfennig, and J. G. Holt (Eds.) Bergey’s Manual of Systematic Bacteriology. Williams and Wilkins. Baltimore, MD. 3:2011–2013.
From: Lysine Iron Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Lysine Iron Agar is used for the differentiation of enteric organisms based on their ability to decarboxylate or deaminate lysine and to form hydrogen sulfide. Principles of the Procedure Dextrose serves as a source of fermentable carbohydrate. The pH indicator, bromcresol purple, is changed to a yellow color at or below pH 5.2 and is purple at or above pH 6.8.8 Ferric ammonium citrate and sodium thiosulfate are indicators of hydrogen sulfide formation. Lysine is the substrate for use in detecting the enzymes, lysine decarboxylase and lysine deaminase. Cultures of enteric bacilli that produce hydrogen sulfide cause blackening of the medium due to the production of ferrous sulfides. Those that produce lysine decarboxylase produce an alkaline reaction (purple color) or neutral reaction in the butt of the medium. Organisms that deaminate the lysine cause the development of a red slant over an acid butt. Gas may be formed but its formation is often irregular or suppressed. Formulae Difco™ Lysine Iron Agar Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine HCl............................................................... 10.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.04 g Bromcresol Purple........................................................ 0.02 g Agar.......................................................................... 15.0 g pH 6.7 ± 0.2 BBL™ Lysine Iron Agar Approximate Formula* Per Liter Pancreatic Digest of Gelatin......................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine...................................................................... 10.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.04 g Bromcresol Purple........................................................ 0.02 g Agar.......................................................................... 13.5 g pH 6.7 ± 0.2
An organic-rich, solid microbiological culture medium containing peptones, yeast extract, glucose, L-lysine, ferric iron, thiosulfate, and a pH indicator (bromcresol purple). Lysine decarboxylation (an anaerobic process) that occurrs in the butt of the culture tube. Decarboxylation causes the color indicator (bromcresol purple) to turn purple due to the production of amines at the bottom of the tube. Posivite lysine decarboxylation: purple tube butt; negative reaction: yellow tube butt. Lysine deamination (an aerobic process) results in the production of ammonia throughout the slant, which will result in an overall red color (due to the reaction of ammonia with ferric ammonium citrate). Positive lysine deamination: red throughout the slant; negative reaction: purple slant. Hydrogen sulfide formation (from thiosulfate reduction) results in the presence of a black precipitate. Positive hydrogen sulfide: black precipitate; negative rection: no precipitate.
An enzymatic assay which tests for the presence of pectinase in a microorganism. Pectinase is a hydrolase enzyme that degrades pectin, a polysaccharide found in the cell walls of plants. Pectin is a heteropolysaccharide comprised mostly of galacturonic acid.
It is not clear which enzyme is being referred to as "lysine deaminase"; here is no such enzyme in GenBank. A putative enzyme in MetaCyc include D-lysine oxidase (studied in Pseudomonas), however the homologs in other Enterobacteriaceae have not been defined. In addition, the distribution of putative orthologs of D-lysine oxidase appear to have a different taxonomic distribution than reported lysine deaminase activity. Another putative enzyme is Lysine dehydrogenase, however homologs in the Enterobacteriaceae are only found in the genomes of Photorhabdus and Yersinia. Another putative enzymatic pathway includes the degradation of lysine to 5-amiopentanamide and 5-aminopentanoate via the enzymes L-lysine monooxygenase and delta-aminovaleramidase. However these enzymes appear to be widely spread in the Enterobacteriaceae, including those that are negative for lysine deaminase activity.
Test for the ability of a microorganism to deaminate lysine. Lysine deamination is an aerobic process.
An enzymatic assay which tests for the presence of malate dehydrogenase in a microorganism.
methyl alpha-D-mannoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl alpha-D-mannoside as a sole source of carbon and energy.
methyl alpha-D-mannopyranoside assimilation
methyl beta-D-xylopyranoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl beta-D-xylopyranoside as a sole source of carbon and energy.
methyl beta-D-xylopyranoside assimilation
Assays for the ability of a microorganism to hydrolyze alpha-amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety. Arylamidases are classes of enzymes with the ability to hydrolyze amino acid-beta-naphtylamide substrates. Sheahan JP, Eitenmiller RR, & Carpenter JA. 1975. Arylamidase activity of Salmonella species. Appl Microbiol 29(6):726-728.
An enzymatic assay which tests for the presence of phosphogluconate dehydrogenase in a microorganism. Phosphogluconate dehydrogenase (phosphogluconate 2-dehydrogenase, 6-phosphogluconic dehydrogenase, gluconate 6-phosphate dehydrogenase, 2-keto-6-phosphogluconate reductase) catalyzes the following reaction: 6-phospho-D-gluconate + NAD(P) <=> 6-phospho-2-dehydro-D-gluconate + NAD(P)H + H+
N-acetylglucosamine-6-phosphate deacetylase assay
N-acetylglucosamine-6-phosphate deacetylase
An enzymatic assay which tests for the presence of N-acetylglucosamine-6-phosphate deacetylase in a microorganism. N-acetylglucosamine-6-phosphate deacetylase catalyzes the following reaction: N-acetyl-D-glucosamine 6-phosphate + H2O <=> D-glucosamine 6-phosphate + acetate
An enzymatic assay which tests for the presence of pyruvate decarboxylase in a microorganism. Pyruvate decarboxylase (also known as alpha-carboxylase, pyruvic decarboxylase, alpha-ketoacid carboxylase, 2-oxo-acid carboxy-lyase) catalyzes the following reactions: a 2-oxo acid <=> an aldehyde + CO2 Pyruvate <=> acetaldehyde + CO2 Pyruvate + thiamine diphosphate <=> 2-(alpha-hydroxyethyl)thiamine diphosphate + CO2 Acetaldehde + thiamine diphosphate <=> 2-(alpha-hydroxyethyl)thiamine diphosphate
An assay for the presence of tetrathionate reductase in a microorganism. Tetrathionate reductase carries out the following set of reactions: tetrathionate + a reduced electron acceptor <=> 2 thiosulfate + an oxidized electron acceptor Tetrationate reduction generates acid, and therefore the pH drops. Some tests look for acid production in the presence of tetrathionate.
Filament septation, where septa separating the individual cells that make up the filament are lacking or are incomplete.
Assays for the ability of a microorganism to assimilate alpha-cyclodextrin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate dextrin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate polysorbate 40 as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate polysorbate 80 as a sole source of carbon and energy.
N-acetyl-beta-D-galactosamine assimilation assay
N-acetyl-beta-D-galactosamine assimilation
Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-galactosamine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate ribitol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-arabinitol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate lactulose as a sole source of carbon and energy.
methyl beta-D-glucopyranoside assimilation assay
methyl-beta-D-glucopyranoside assimilation
Assays for the ability of a microorganism to assimilate methyl-beta-D-glucopyranoside as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-psicose as a sole source of carbon and energy.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium9.pdf 9. VY/2 AGAR Baker's yeast 5.00 g CaCl2 x 2 H2O 1.36 g Vitamin B12 0.50 mg Agar (Difco) 15.00 g Distilled water 1000.00 ml Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50˚C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material. For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 9, except the concentration of cobalamin is decreased.
DSM 11116 is Hymenobacter gelipurpurascens
succinic acid mono-methyl ester assimilation
Assays for the ability of a microorganism to assimilate monomethyl succinate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate formate (formic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-galactono-1,4-lactone or D-galactono-1,5-lactone as a sole source of carbon and energy.
D-galactonic acid gamma-lactone assimilation
2-amino-2-deoxy-D-gluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-amino-2-deoxy-D-gluconate (2-amino-2-deoxy-D-gluconic acid) as a sole source of carbon and energy.
2-hydroxybutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-hydroxybutyrate (2-hydroxybutyric acid, alpha-hydroxybutyric acid) as a sole source of carbon and energy.
4-hydroxybutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-hydroxybutyrate (4-hydroxybutyric acid) as a sole source of carbon and energy.
4-hydroxyphenylacetic acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-hydroxyphenylacetate (4-hydroxyphenylacetic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2-oxobutanoate (2-oxobutanoic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2-oxoglutaric acid (2-oxoglutarate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2-oxopentanoate (2-oxopentanoic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate quinate (quinic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-glucarate (D-glucaric acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate sebacate (sebacic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate bromosuccinate (bromosuccinic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate succinamic acid as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glucuronamide (beta-D-glucuronamide) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-alaninamide as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-alanine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-alanyl-glycine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-asparagine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-aspartate (L-aspartic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycyl-L-aspartate (glycyl-L-aspartic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycyl-L-glutamate (glycyl-L-glutamic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate hydroxyproline as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-leucine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-ornithine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-phenylalanine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 5-oxoprolinate (5-oxoproline) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-serine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-threonine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D,L-carnitine as a sole source of carbon and energy.
gamma-aminobutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate gamma-aminobutyrate (gamma-aminobutyric acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate urocanate (urocanic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate inosine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate uridine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate thymidine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 1-phenylethylamine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate ethanolamine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2,3-butanediol (butane-2,3-diol) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycerol phosphate as a sole source of carbon and energy.
alpha-D-glucose 1-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate alpha-D-glucose 1-phosphate as a sole source of carbon and energy.
alpha-D-glucose 1-phosphate assimilation
D-glucose 6-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate D-glucose 6-phosphate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate beta-cyclodextrin as a sole source of carbon and energy.
Biolog, Inc. is a company that designs cell-based phenotypic testing technologies and assays for microorganisms.
Biolog SF-N2 MicroPlate™ is designed for the metabolic testing of Sporulating and Filamentous (SF) microorganisms such as actinomycetes and fungi. Cells are resuspended in a gel (of gellan gum or carraghenan) to prevent clumping and uneven cell distributions. Cells also do not contain tetrazoleum violet. A positive reading is determined by growth of the organism on a single carbon source as determined by the appearance of turbidity. The tests performed are the same as those on the GN2 plate.
Biolog GN2 MicroPlate™ is designed to identify a wide variety of gram-negative bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. The tests performed are the same as those on the Biolog SF-GN2 plate.
Assays for the ability of a microorganism to assimilate inulin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate mannan as a sole source of carbon and energy.
N-acetyl-D-mannosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetyl-D-mannosamine as a sole source of carbon and energy.
methyl alpha-D-galactoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl alpha-D-galactoside as a sole source of carbon and energy.
methyl alpha-D-galactopyranoside assimilation
methyl a-D-galactopyranoside assimilation
methyl beta-D-galactoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl beta-D-galactoside as a sole source of carbon and energy.
methyl beta-D-galactopyranoside assimilation
methyl b-D-galactopyranoside assimilation
Assays for the ability of a microorganism to assimilate 3-O-methyl-D-glucose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate sedoheptulosan as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate stachyose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate D-tagatose as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate lactamide as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate methyl (R)-lactate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate (R)-malate(2-) as a sole source of carbon and energy.
N-acetyl-L-glutamic acid assimilation assay
Assays for the ability of a microorganism to assimilate N-acetyl-L-glutamate (N-acetylglutamic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate adenosine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2'-deoxyadenosine as a sole source of carbon and energy.
adenosine 5'-monophosphate assimilation assay
Assays for the ability of a microorganism to assimilate adenosine 5'-monophosphate as a sole source of carbon and energy.
thymidine 5'-monophosphate assimilation assay
Assays for the ability of a microorganism to assimilate thymidine 5'-monophosphate as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate UMP as a sole source of carbon and energy.
D-fructose 6-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate D-fructose 6-phosphate as a sole source of carbon and energy.
Biolog SF-P2 MicroPlate™ is designed for the metabolic testing of Sporulating and Filamentous (SF) microorganisms such as actinomycetes and fungi. Cells are resuspended in a gel (of gellan gum or carraghenan) to prevent clumping and uneven cell distributions. Cells also do not contain tetrazoleum violet. A positive reading is determined by growth of the organism on a single carbon source as determined by the appearance of turbidity. The tests performed are the same as those on the GP2 plate.
Biolog SF-P2 MicroPlate™ is designed to identify a broad range of gram positive bacteria Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources. The tests performed are the same as those on the SF-P2 plate.
An assay for the ability of a microorganism to grow in a microbiological culture medium at distinct pH values.
An assay for the ability of a microorganism to grow at pH 6.0.
An assay for the ability of a microorganism to grow at pH 5.0.
An assay for the ability to grow in the presence of 1.0% sodium chloride (NaCl).
An assay for the ability to grow in the presence of 4.0% sodium chloride (NaCl).
An assay for the ability to grow in the presence of 8.0% sodium chloride (NaCl).
N-acetylneuraminic acid assimilation assay
Assays for the ability of a microorganism to assimilate N-acetylneuraminic (N-acetylneuraminate) acid as a sole source of carbon and energy.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of fusidic acid.
Assays for the ability of a microorganism to assimilate D-aspartate (D-aspartic acid) as a sole source of carbon and energy.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of troleandomycin.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of rifamycin SV.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of minocycline.
Assays for the ability of a microorganism to assimilate gelatin as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycyl-L-proline as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-arginine as a sole source of carbon and energy.
An assay for the ability of a microorganism to grow in the presence of a chemical (microbiocides, surfactants, inorganic substance, organic substance, or antibiotics).
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of lincomycin.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of guanidine (guanidinium), a protein denaturant.
An assay for the ability of a microorganism to grow or utilize sugars in the presence of a potentially growth inhibiting chemical (an antimicrobial compound).
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of Niaproof 4 (Tergitol® 4; sodium tetradecyl sulfate), a anionic detergent.
Assays for the ability of a microorganism to assimilate pectin as a sole source of carbon and energy.
L-galactono-1,4-lactone assimilation assay
Assays for the ability of a microorganism to assimilate L-galactono-1,4-lactone as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate galactaric acid (galactarate) as a sole source of carbon and energy.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of vancomycin.
An assay for the ability of a microorganism to reduce tetrazolium violet, a redox indicator of microbial growth. Tetrazolium violet is a yellow-green color, however, when reduced it forms a purple color.
An assay for the ability of a microorganism to reduce tetrazolium blue, a redox indicator of microbial growth. Tetrazolium blue is colorless, however, when reduced it forms a blue tetrazoleium diformazan.
Assays for the ability of a microorganism to assimilate methyl pyruvate as a sole source of carbon and energy.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of (potassium) tellurite.
Assays for the ability of a microorganism to assimilate acetoacetate (acetoacetic acid) as a sole source of carbon and energy.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of lithium chloride.
An antibiotic resistance assay for the ability of a microorganism to grow in the presence of aztreonam.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of sodium butyrate (butyric acid).
A chemcial sensitivity assay for the ability of a microorganism to grow int he presence of (sodium) bromate.
Biolog Gen III MicroPlate™ is designed to identify a broad range of gram-negative and gram-positive bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources.
Assays for the ability of a microorganism to assimilate D-fucose as a sole source of carbon and energy.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of 1% sodium lactate.
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of D-serine.
Biolog AN MicroPlate™ is designed to identify a very wide range of anaerobic bacteria. Tests for the ability of a microorganism to utilize or oxidize compounds from a preselected panel of different carbon sources. Tetrazolium voilet is used as a redox dye to colorimetrically indicate utilization of the carbon sources.
Assays for the ability of a microorganism to assimilate fumaric acid (fumarate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glyoxylic acid (glyoxylate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate meso-tartrate (meso-tartaric acid) as a sole source of carbon and energy.
L-alanyl-L-glutamic acid assimilation assay
Assays for the ability of a microorganism to assimilate L-alanyl-L-glutamate (L-alanyl-L-glutamic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-alanyl-L-histidine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-alanyl-L-threonine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate glycyl-L-methionine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-methionine as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate L-valine as a sole source of carbon and energy.
L-valine plus L-aspartate assimilation assay
Assays for the ability of a microorganism to assimilate L-valine plus L-aspartate (L-valine plus L-aspartic acid) as a sole source of carbon and energy.
L-valine plus L-aspartic acid assimilation
Assays for the ability of a microorganism to assimilate glycyl-L-glutamine as a sole source of carbon and energy.
RapID™ microbiogy test kits. Manufactured and sold variously by Innovative Diagnostics Systems Inc., Remel Inc., and now ThermoScientific.
RapID™ ANA II System, designed to identify over 90 medically imporant anaerobes.
RapID™ NH System, designed to identify over 30 taxa including Neisseria, Moraxella, Haemophilus, and related microorganisms.
The optical quality of a liquid culture of clonal prokaryotic organisms, where the culture medium has a distinctive color that is a result of the prokaryotic production of a pigment compound.
RapID™ CB Plus System, designed to identify Corynebacteria.
Schmid M, Berg M, Hilbi H, Dimroth P. 1996. Malonate decarboxylase of Klebsiella pneumoniae catalyses the turnover of acetyl and malonyl thioester residues on a coenzyme-A-like prosthetic group. Eur J Biochem 237(1):221-228.
Assays for the ability of a microorganism to assimilate malonate (malonic acid) as a sole source of carbon. Assimilation of malonate results in an increase in pH (alkalinization) as a result of decarboxylation of malonate into acetate and CO2 via malonate decarboxylase. Involves two enzymes: Malonyl-CoA synthetase (malonate + ATP + CoA = malonyl-CoA + AMP + PPi) Malonyl-CoA decarboxylase (malonyl-CoA = acetyl-CoA + CO2)
RapID™ ONE System, designed for the identification of over 70 medically important Enterobacteriaceae.
RapID™ STR System, designed for the identification of Streptococci and related genera.
An assay for the presence of thiosulfate disproportionation, followed by reduction, in a microorganism. Thiosulfate reduction (for example; there are several pathways in microorganisms) can involve the following sets of reactions: thiosulfate -> sulfate + H2S + H+ Sulfate, in turn, may be reduced to sulfide, generating more H+ ions. Thiosulfate reduction generates acid, and therefore the pH drops. Some assays look for acid production in the presence of thiosulfate.
An amino acid arylamidase assay that uses the substrate N-benzyl-arginine-2-naphthylamide (N-benzyl-arginine-beta-naphthylamide) at pH 7.5. Benzyl-arginine arylamidase activity (which could be from benzyl-arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
RapID™ NF Plus System, designed for the identification of over 70 medically imporant, oxidase-positive, gram-negative bacilli, including Neisseria, Haemophilus, and Vibrio spp.
RapID™ Staph Plus System, designed for the identification of over 40 staphylococci and related genera.
A mineral-salts, liquid microbiological culture medium for the discrimination of sugar utilization (fermentation) in bacilli. Bacilli may not produce enough acids in conventional peptone-containing medium due to the production of ammonium from the deamination of peptides. Thus, ammonium salt sugar medium contains yeast extract. A positive assay for carbohydrate fermentation is yellow due to a low pH; a negative assay is purple.
From: Snell JJS, Lapage SP. 1971. Comparison of four methods for demonstrating glucose breakdone in bacteria. J Gen Microbiol 68:221-225. "Ammonium salt sugar (ASS) (Smith, Gordon & Clark, 1952). (NH4),HP04, 1 g.; KCl, 0.2 g. ; MgSO4*7H,O, 0.2 g. ; Yeastrel, 0.2 g. ; agar, 12 g. ; bromocresol purple, 4 ml. of a 1 % (w/v) aqueous solution; glucose, 20 ml. of a 50 % (w/v) Seitz filtered solution; distilled water, 1 1. The solids were dissolved in the water by heating, the pH was adjusted to 7.0 and the indicator added. The medium was sterilized at 115˚ for 15 min. and after cooling to 50˚the sterile glucose solution was added." Note: Yeastrel was a brand of yeast extract (no longer manufactured).
A microbiological diagnostic assay for the requirement of growth factors (hemin, coenzyme I) to support growth by a microorganism. These growth factors are used for the differentiation of Haemophilus and Bordetella species. The growth factors can be added to the culture medium directly, or they can be added using growth factors impregnated into paper discs.
Paper disc impregnated with the growth factor haemin (hemin).
Paper disc impregnated with the growth factor NAD (nicotinamide adenine dinucleotide).
Paper disc impregnated with the growth factors haemin (hemin) and NAD (nicotinamide adenine dinucleotide).
A beta-hemolysis assay used to identify Streptococcus agalactiae (Group B) and to differentiate it from Streptococcus pyrogenes (Group A) and other (Group C to Group H) streptococci. Group B streptococci are CAMP positive; Group A and Nongroup B streptococci are CAMP negative. The assay is performed on Trypticase Soy Agar plates with sheep blood. The test/unknown Streptococcus organism is streaked onto TSA plates at right angles to the beta-lysin positive Staphycoccus aureus. In a positive test (CAMP +) outcome, enhanced zones of clearing will be seen around the test organism when it is near the Staph. aureus. This means that there is a synergistic respones between the test organism and the beta-lysin secreted by the Staph. aureus. In a negative test (CAMP - ) outcome, no enhanced zone of clearing will be seen.
From: Indole Nitrate Medium/Trypticase Nitrate Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Indole Nitrite Medium is used for the identification of microorganisms by means of the nitrate reduction and indole tests. Principles of the Procedure The casein peptone contains tryptophan, which is attacked by certain microorganisms, resulting in the production of indole, detectable by the addition of chemical reagents to 18- to 48-hour cultures. Potassium nitrate serves as the substrate for determining the ability of microorganisms to reduce nitrates to nitrites. Formula BBL™ Indole Nitrite Medium (Trypticase™ Nitrate Broth) Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 20.0 g Disodium Phosphate.................................................... 2.0 g Dextrose...................................................................... 1.0 g Agar............................................................................ 1.0 g Potassium Nitrate......................................................... 1.0 g *Adjusted and/or supplemented as required to meet performance criteria. pH 7.2 ± 0.2
An organic-rich, liquid microbiological culture medium containing pancreatic digest of casein, a phosphate buffer, glucose, and nitrate. Used to test for nitrate reduction and production of indole.
An organic-rich, solid microbiological culture medium containing pancreatic digest of casein, yeast extract, the dye Spirit Blue (a dye indicator of lipolysis), and Spirit Blue Lipase Reagent (contains tributyrin, a triglyceride, and polysorbate 80, an emulsifying agent). Microorganisms with lipolytic activity form colonies with halos.
From: Spirit Blue Agar / Lipase Reagent (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Spirit Blue Agar is for use with Lipase Reagent or other lipid source for detecting and enumerating lipolytic microorganisms. Principles of the Procedure Spirit Blue Agar contains peptone as a source of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Spirit blue is the indicator of lipolysis. Agar is the solidifying agent. Lipase Reagent contains tributyrin, a true fat and the simplest triglyceride occurring in natural fats and oils. It is a good substrate when testing for lipolytic microorganisms because some microorganisms that hydrolyze tributyrin will not hydrolyze other triglycerides or fats containing longer chain fatty acids.2 Formulae Difco™ Spirit Blue Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Yeast Extract ............................................................... 5.0 g Agar.......................................................................... 20.0 g Spirit Blue.................................................................... 0.15 g Difco™ Lipase Reagent A ready-to-use lipid suspension, containing a mixture of tributyrin and polysorbate 80. *Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2
An assay for the ability of a microorganism to hydrolyze starch, using Starch Agar, a medium designed to test for the ability to produce alpha-amylase and oligo-1,6-glucosidase. The agar is flooded with iodine after colonies have developed on the plate, turning starch a dark color. Clearing around colonies indicates starch hydrolysis.
An assay to determine whether a microorganism is motile. Organisms are inoculated into a semi-solid medium. Organisms that are motile will migrate away from the initial inoculum. Medium often includes a redox indicator (such as triphenyltetrazolium chloride) which is reduced producing a red dye which helps show the extend of microbial growth.
From: Starch Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Starch Agar is used for cultivating microorganisms being tested for starch hydrolysis. Principles of the Procedure Beef extract provides the nitrogen, vitamins, carbon and amino acids in Starch Agar. Starch reacts with Gram Iodine to give a blue color. Organisms hydrolyzing starch through amylase production will produce a clearing around the isolate while the remaining medium is blue. Agar is the solidifying agent. Formula Difco™ Starch Agar Approximate Formula* Per Liter Beef Extract.................................................................. 3.0 g Soluble Starch............................................................ 10.0 g Agar.......................................................................... 12.0 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 25 g of the powder in 1 L of purified water. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Test samples of the finished product. pH 7.5 ± 0.2 Procedure Starch Hydrolysis Test Flood the surface of a 48-hour culture on Starch Agar with Gram Iodine. Expected Results Starch hydrolysis (+) is indicated by a colorless zone surrounding colonies. A blue or purple zone indicates that starch has not been hydrolyzed (-).
An organic-rich, solid microbiological culture medium containing beef extract and starch. Used to cultivate heterotrophic microorganisms and test for the ability to hydrolyze starch.
A microbiological assay of the promotion of blood clotting which assays for the ability of a microorganism to secrete the enzyme coagulase which converts fibrinogen to fibrin and causes clotting of blood plasma.
Coagulase assay performed on a glass slide, is used to identify the presence of coagulase that is bound to the cell wall of the test microorganism. Cells are emulsified into two drops of saline on a slide. A drop of plasma is added to one of the drops and mixed into the cell suspension. Coagulase reacts with fibrinogen in blood plasma, causing the cells to agglutinate or clump together after 10 seconds. The drop with no plasma acts as a negative control. A positive test shows cells clumping; a negative test shows no clumping. Clumping in the negative control is an indication of autoagglutination.
Coagulase assay performed in a test tube, is used to identify the presence of coagulase that is bound to the cell wall of the test microorganism or is free of the cell. A microbial colony is suspended in EDTA-treated plasma and incubated for 1-2 hours to permit microbial growth in the plasma. Some species of microbes are able to secrete coagulase into the plasma, forming a clot. Clot formation can take up to 24 hours. A negative control tube is performed in the absence of cells. A positive test shows clot formation; a negative test shows no clot formation.
An organic-rich, solid microbiological culture medium that contains peptones, two sugars (glucose and lactose), ferric iron, thiosulfate, and phenol red (a pH indicator). If the sugars are fermented, the pH indicator will turn red indicating a lowered pH. If hydrogen sulfide is produced, the ferric iron will be reduced to ferrous sulfide, producing a black color.
From: Kligler Iron Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Kligler Iron Agar is used for the differentiation of members of the Enterobacteriaceae on the basis of their ability to ferment dextrose and lactose and to liberate sulfides. Principles of the Procedure Kligler Iron Agar, in addition to casein and meat peptones, contains lactose and dextrose which enable the differentiation of species of enteric bacilli due to color changes of the phenol red pH indicator in response to the acid produced during the fermentation of these sugars. The dextrose concentration is only 10% of the lactose concentration. The combination of ferric ammonium citrate and sodium thiosulfate enables the detection of hydrogen sulfide production. Lactose nonfermenters (e.g., Salmonella and Shigella) initially produce a yellow slant due to acid produced by the fermentation of the small amount of dextrose. When the dextrose supply is exhausted in the aerobic environment of the slant, the reaction reverts to alkaline (red slant) due to oxidation of the acids. The reversion does not occur in the anaerobic environment in the butt, which remains acid (yellow butt). Lactose fermenters produce yellow slants and butts because enough acid is produced in the slant to maintain an acid pH under aerobic conditions. Organisms incapable of fermenting either carbohydrate produce red slants and butts. Hydrogen sulfide production is evidenced by a black color either throughout the butt, or in a ring formation near the top of the butt. Gas production (aerogenic reaction) is detected as individual bubbles or by splitting or displacement of the agar. Formula BBL™ Kligler Iron Agar Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 10.0 g Peptic Digest of Animal Tissue.................................... 10.0 g Lactose...................................................................... 10.0 g Dextrose...................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Ferric Ammonium Citrate............................................. 0.5 g Sodium Thiosulfate...................................................... 0.5 g Agar.......................................................................... 15.0 g Phenol Red................................................................ 25.0 mg *Adjusted and/or supplemented as required to meet performance criteria. pH 7.4 ± 0.2 Procedure To inoculate, carefully touch the center of an isolated colony on an enteric plated medium with a cool, sterile needle, stab into the medium in the butt of the tube, and then streak back and forth along the surface of the slant. Several colonies from each primary plate should be studied separately, since mixed infections may occur. Incubate tubes with loosened caps for 18-24 hours at 35 ± 2°C in an aerobic atmosphere.To enhance the alkaline condition in the slant, free exchange of air must be permitted through the use of a loose closure. If the tube is tightly closed, an acid reaction (caused solely by dextrose fermentation) will also involve the slant. Expected Results After incubation, record the reaction in the slant and butt, noting gas formation and hydrogen sulfide production.
alpha-L-arabinofuranosidase assay with pNP
An assay for the activity of alpha-arabinosidase in a microorganism. Uses the substrate 4-nitrophenyl-alpha-L-arabinofuropyranoside. Alpha-arabinosidase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
alpha-L-arabinofuranosidase assay with 4-MU
An assay for the activity of alpha-L-arabinofuranosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-alpha-L-arabinofuropyranoside. Alpha-L-arabinofurosidase will cleave the substrate, producing 4-methylumbelliferone which is highly fluorescent. A positive result is highly fluorescent; a negative result is non-fluorescent.
A microbiological diagnostic assay for the ability of a microbial colony to develop an irridescent film when grown on egg yolk agar. The irridescence is due to the presence of lipase, which causes the breakdown of fats (triglycerides into free fatty acids) in the egg yolk.
An assay for the ability of a microbial colony to develop crystalline spots, or a fine crystalline deposit, when grown on egg yolk agar. The spots are due to precipitation of calcium and magnesium "soaps" that are usually associated with a positive film reaction (i.e. the relase of fatty acid anions by lipolytic activity that combine with free calcium and magnesium in the medium).
growth is inhibited on medium containing 20% bile
A chemcial sensitivity assay for the ability of a microorganism to grow in the presence of bile (bile salts).
growth not inhibited in the presence of 20% bile
An assay where cells from a culture or a colony are stained with a dye which will differentially stain (i.e. bind to and be retained in) various chemical components of the cell.
Cell staining assay where a test for how the stain Congo Red stains microbial cells or microbial cell components. Congo Red is adsorbed by cellulose and unusual lipopolysaccharide structures. Can be used to stain cells or colonies.
Assays for the ability of a microorganism to ferment citrate (citric acid).
Assays for the ability of a microorganism to ferment malonate (malonic acid).
Assays for the ability of a microorganism to ferment amygdalin.
An organic-rich liquid medium intended to test for the ability of a microorganism to decarboxylate amino acids, through use of pH indicator compounds. Pyridoxal is added because it is an enzymatic co-factor for amino acid decarboxylases. Peptones and beef/yeast extract and dextrose (D-glucose) are added to promote growth. Lysine, ornithine, or arginine are added to test for decarboxylation.
From: Decarboxylase Differential Media (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Decarboxylase media are used in the biochemical differentiation of gram-negative enteric bacilli based on the production of arginine dihydrolase and lysine and ornithine decarboxylase. Decarboxylase Medium Base, with added arginine, lysine or ornithine is used for the same purpose. Lysine Decarboxylase Broth is used for differentiating microorganisms based on lysine decarboxylation. Procedure Inoculate the broth media by transferring one or two colonies from the surface of a fresh culture with an inoculating loop or needle and mix to distribute the culture throughout the medium. Overlay the medium in each tube with 1 mL sterile mineral oil. Incubate the tubes with caps tightened at 35 ± 2°C. Examine for growth and decarboxylase reactions after 18-24, 48, 72 and 96 hours before reporting as negative. The medium will become yellow initially, if the dextrose is fermented, and then will gradually turn purple if the decarboxylase or dihydrolase reaction occurs and elevates the pH. Expected Results Compare the color of tubes of media containing the specific amino acids with the color of control tubes of basal media (without amino acid) that have been inoculated with the same isolate. If inoculated control tubes show an alkaline reaction, the test is invalid; i.e., either improperly performed or the test organisms can degrade the peptone sufficiently to produce an alkaline reaction in the absence of a specific amino acid. The medium becomes purple to violet if the reaction is positive (alkaline). A yellow color indicates a negative test; i.e., the organism does not produce the appropriate enzyme. Formulae Difco™ Decarboxylase Base Moeller Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Beef Extract.................................................................. 5.0 g Dextrose...................................................................... 0.5 g Bromcresol Purple........................................................ 0.01 g Cresol Red................................................................... 5.0 mg Pyridoxal...................................................................... 5.0 mg pH 6.0 ± 0.2 BBL™ Moeller Decarboxylase Broth Base Approximate Formula* Per Liter Peptic Digest of Animal Tissue...................................... 5.0 g Beef Extract.................................................................. 5.0 g Dextrose...................................................................... 0.5 g Bromcresol Purple........................................................ 0.01 g Cresol Red................................................................... 5.0 mg Pyridoxal...................................................................... 5.0 mg Difco™ Decarboxylase Medium Base Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g Bromcresol Purple........................................................ 0.02 g pH 6.8 ± 0.2 Difco™ Lysine Decarboxylase Broth Approximate Formula* Per Liter Peptone....................................................................... 5.0 g Yeast Extract................................................................ 3.0 g Dextrose...................................................................... 1.0 g L-Lysine........................................................................ 5.0 g Bromcresol Purple........................................................ 0.02 g * Adjusted and/or supplemented as required to meet performance criteria. pH 6.8 ± 0.2
Decarboxylase differential medium containing lysine. Designed to determine whether a microorganism has the ability to decarboxylate lysine.
Moeller decarboxylase broth with ornithine
Decarboxylase differential medium containing ornithine. Designed to determine whether a microorganism has the ability to decarboxylate ornithine.
Moeller decarboxylase broth with arginine
Decarboxylase differential medium containing arginine. Designed to determine whether a microorganism has the ability to decarboxylate arginine.
An aminopeptidase activity which cleaves the chromogenic substrate L-leucine-4-methoxy-2-naphthylamide (4-methoxy-leucyl-beta-naphthylamide, L-leucine 4-methoxy-beta-naphthylamide).
anaerobic respiration, using nitrate as electron acceptor
The process of anaerobic respiration, where nitrate is reduced to nitrite, nitric oxide, nitrous oxide, or dinitrogen.
anaerobic respiration, using nitrite as electron acceptor
The process of anaerobic respiration, where nitrite is reduced to nitric oxide, nitrous oxide, or dinitrogen.
A substance derived from the treatment of chicken egg yolks with a salt solution, followed by filtration.
An aqueous (water) extract of whole chicken egg, used in the cultivation of microorganisms.
microbiological medium ingredient, derived from animal blood
Undefined mixture of complex organic compounds comprising a microbiological culture medium ingredient derived from the blood or blood plasma from a mammal. Used to support the growth of microorganisms which need blood to grow.
Blood medium ingredient comprised of blood from a rabbit (Orychtolagus cuniculus), which has been laked (treated so that the red blood cells have undergone haemolysis). Used to support the growth of microorganisms which need rabbit laked blood to grow.
Whole blood from a rabbit (Oryctolagus cuniculus). Used to support the growth of microorganisms which need rabbit blood to grow.
Whole blood from a sheep (Ovis aries). Used to support the growth of microorganisms which need sheep's blood to grow.
Blood medium ingredient comprised of red blood cells from a mammal that have been washed. Used to support the growth of microorganisms which need washed red blood cells to grow.
Blood medium ingredient comprised of the blood of a mammal that have been lysed (undergone the process of cytolysis). Used to support the growth of microorganisms which need lysed red blood cells to grow.
A microbiological culture medium ingredient derived from the blood from a mammal. Used to support the growth of microorganisms which need blood to grow.
Whole blood of a horse (Equus caballus). Used to support the growth of microorganisms which need horse blood to grow.
Serum medium ingredient comprised of blood serum from a horse (Equus caballus). Used to support the growth of microorganisms which need horse blood serum to grow.
A microbiological culture medium ingredient derived from the blood serum from a mammal. Used to support the growth of microorganisms which need blood serum to grow.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Serum is the clear yellowish fluid obtained when whole blood is separated into its liquid and solid components. Blood is allowed to clot so that the serum separates from the blood cells. The liquid portion released from the clot is called serum and does not contain fibrinogen as the fibrinogen was utilized to form the fibrin threads of the blood clot. Serum usually is inactivated by heating to 56 degrees C. for 30 minutes to eliminate lipases that would cause degradation of lipids and inactivation of complement.
Blood medium ingredient comprised of the blood from a mammal that has been laked (treated so that the red blood cells have undergone haemolysis). Used to support the growth of a microorganisms which needs laked blood to grow. Is an ingredient for blood agar.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Laked Blood is defibrinated blood that has been processed with freeze/thaw cycles to hemolyze the red blood cells. It is clear, red, and homogenous and provides many nutrients. It is useful for growing fastidious organisms due to its highly available nutrient contents.
Serum medium ingredient comprised of blood serum from a pig (Sus scrofa). Used to support the growth of microorganisms which need porcine serum to grow.
microbiological culture medium containing bile
A microbiological culture medium that contains bile (fluid produced by the liver) or bile salts (such as deoxycholate). Used for the culture of microorganisms that require bile.
Undefined mixtures of complex organic compounds derived from the whole milk of a mammal. Used in the cultivation of microorganisms.
Microbiological medium ingredient, derived from milk. Comprised of skimmed milk which has been dried to a powder.
Microbiological medium ingredient, derived from milk. Comprised of a heterogeneous mixture of milk proteins (casein) of a mammal. Used in the cultivation of microorganisms.
microbiological medium ingredient, derived from milk
Undefined mixtures of complex organic compounds derived from the milk of a mammal. Used in the cultivation of microorganisms.
An aminopeptidase activity which cleaves alpha-amino acid substrates coordinated with a beta-naphthylamide (2-naphthylamide) moiety.
An aminopeptidase activity which cleaves the chromogenic substrate DL-methionine-2-naphthylamide (DL-methionyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-glycine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate alanine-2-naphthylamide (L-alanine-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-arginine-2-naphthylamide (L-arginine-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-asparagine-2-naphthylamide (L-asparaginyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-aspartate-2-naphthylamide (L-aspartic acid-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-cysteine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-cystine-di-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-alpha-glutamate-2-naphthylamide (L-alpha-glutamyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-glutamate-2-naphthylamide (L-glutamyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-glutamine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-histidine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-hydroxyproline-2-naphthylamide (L-hydroxyprolyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-isoleucyl-2-naphthylamide (L-isoleucine-beta-naphthylamide) .
An aminopeptidase activity which cleaves the chromogenic substrate L-leucyl-2-naphthylamide (L-leucine-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-lysine-2-naphthylamide (L-lysyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-methionine-2-naphthylamide (L-methionyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-ornithine-2-naphthylamide (L-ornithyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-phenylalanine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-proline-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate pyroglutamic acid-2-naphthylamide (pyroglutamic acid-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-serine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-threonine-2-naphthylamide (L-threonyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-tryptophan-2-naphthylamide (L-tryptophyl-beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate L-tyrosine-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-valyl-2-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate beta-alanine-2-naphthylamide (beta-alanine beta-naphthylamide).
An aminopeptidase activity which cleaves the chromogenic substrate gamma-glutamyl-4-methoxy-beta-naphthylamide.
An aminopeptidase activity which cleaves the chromogenic substrate L-gamma-glutamate-2-naphthylamide (L-gamma-glutamyl-beta-naphthylamide.
A set of microbial enzymatic reactions that result in the deamination of lysine.
From: Phenylalanine Agar; Ferric Chloride Reagent (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Phenylalanine Agar is used for the differentiation of enteric bacilli on the basis of their ability to produce phenylpyruvic acid by oxidative deamination. Ferric Chloride Reagent is used to visualize the phenylalanine deamination reaction. Principles of the Procedure The phenylalanine serves as the substrate for enzymes which are able to deaminate it to form phenylpyruvic acid. The addition of 3-5 drops of a 10% aqueous ferric chloride solution (or a 12% aqueous ferric chloride solution acidified with 2.5 mL of concentrated HCl per 100 mL of reagent) to the cultures following incubation results in the appearance of a light to deep green color (positive reaction) or no color change (negative reaction). In a positive reaction, any phenylpyruvic acid present will react with the ferric salt in the reagent to give a green color. Formulae Difco™ Phenylalanine Agar Approximate Formula* Per Liter DL-Phenylalanine.......................................................... 2.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Dipotassium Phosphate................................................ 1.0 g Agar.......................................................................... 12.0 g pH 7.3 ± 0.2 BBL™ Phenylalanine Agar Approximate Formula* Per Liter DL-Phenylalanine.......................................................... 2.0 g Yeast Extract................................................................ 3.0 g Sodium Chloride.......................................................... 5.0 g Sodium Phosphate....................................................... 1.0 g Agar.......................................................................... 12.0 g pH 7.3 ± 0.2 Difco™/BBL™ Ferric Chloride Reagent Droppers Contain 0.5 mL of 10% ferric chloride in aqueous solution.
An organic-rich, solid microbiological culture medium containing DL-phenylalanine, yeast extract and sodium chloride. Used to test for the formation of phenylpyruvic acid.
Prokaryotic cell wall lysis susceptibility that defines how susceptible the cell wall is to lysis when the cell is placed in the presence of an environment with an altered chemical composition (plain water).
Wikipedia: Tonicity Hypotonicity Hypotonic refers to a lesser concentration. In biology, a hypotonic solution has a lower concentration of solutes outside the cell than inside the cell. In an attempt to balance the concentrations of solutes inside and outside the cell, water will rush into the cell, and can cause it to burst.[2]
A chemical solution that has a lower concentration of solutes than the concentration inside a cell.
A solution of an inorganic salt dissolved in water.
Filament differentiation quality, relating to the morphology and differentiation of the sub-apical cell in the filament.
Trichome cell, which is in the medial region of the trichome, which has not undergone differentiation.
Trichome part defined by a single individual cell, which may or may not have undergone differentiation, within the trichome.
Temperature optimum quality, growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures, but will tolerate higher temperatures.
Thermophilic, can only grow at elevated temperatures (40-85˚C).
Culture medium quality with respect to the concentration of salt ions (salinity).
Culture medium salinity quality where the salinity is less than 0.05 % salts.
Culture medium salinity quality where the salinity is greater than 0.05 % salts and less than 3 % salts.
Culture medium salinity quality where the salinity is greater than 3 % salts and less than 5 % salts.
Culture medium salinity quality where the salinity is greater than 5 % salts.
Temperature optimum quality, where growth rates at highly elevated temperatures (>85˚C) are higher than growth rates at lower temperatures.
A cell division pattern where the plane of cell division is always parallel to the previous plane of cell division.
A cell division pattern where the plane of cell division can occur in two planes, both at right angles to one another.
A cell division pattern where the plane of cell division can occur in three planes, all three being orthogonal (at right angles) to each other.
A cell division pattern where the plane of cell division can occur in random planes.
Michelle A. Allen,Falicia Goh,Stefan Leuko,Akinobu Echigo,Toru Mizuki,Ron Usami,Masahiro Kamekura, Brett A. Neilan and Brendan P. Burns. 2008. Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia. IJSEM 28:798-802. A halophilic archaeal medium [containing (g/L): Casamino acids, 7.5; yeast extract, 10.0; trisodium citrate, 3.00; NaCl, 150; KCl, 2.00; MgSO4 . 7H2O, 20.0; MgCl2 . 6H2O, 7.23; CaCl2 . 2H2O, 2.70; FeSO4 . 7H2O, 0.05; MnSO4.H2O, 0.0002; adjusted to pH 7.4; Goh et al., 2006] solidified with agar
A hypersaline, organic-rich, liquid culture medium containing mineral-salts, magnesium sulfate, citrate, casamino acids and yeast extract. Used for the cultivation of Haloferax.
A hypersaline, liquid microbiological culture medium containing mineral-salts, pyruvate, lactate, formate, acetate, peptone, and yeast extract. Used for the cultivation of Halolamina pelagica and Halopelagius longus.
From: Heng-Lin Cui, Xia Gao, Xin Yang and Xue-Wei Xu. 2011. Halolamina pelagica gen. nov., sp. nov., a new member of the family Halobacteriaceae. IJSEM 61:1617-1621. 1.0 g yeast extract 0.25 g fish peptone 0.25 g sodium pyruvate, 0.25 g sodium formate 0.25 g sodium acetate 0.25 g sodium lactate 5.4 g KCl, 0.3 g K2HPO4, 0.25 g CaCl2, 0.25 g NH4Cl, 26.8 g MgSO4 . 7H2O, 23.0 g MgCl2 . 6H2O and 184.0 g NaCl (adjusted to pH 7.0–7.2 with 1 M NaOH)
cellulolytic rumen bacteria vitamin solution
From: Scott HW, Dehority BA. 1965. Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89(5):1169-1175. Vitamins listed From Table 1 (mg per 100 mL) Pyridoxine hydrochloride 0.2 Riboflavine 0.2 Thiamine hydrochloride 0.2 Nicotinamide 0.2 Ca-D-pantothenate 0.2 p-Aminobenzoic acid 0.01 Folic acid 0.005 Biotin 0.005 B12 0.0005
Microbiological vitamin solution, used to support the growth of cellulolytic rumen bacteria. Contains pyridoxine hydrochloride, riboflavin, thiamine, nicotinamide, pathothenate, PABA (i.e. 4-aminobenzoic acid), biotin, and vitamin B12 (i.e. cobalalmin).
Hyperthermophilic, can only grow at highly elevated temperatures (>85˚C).
Temperature optimum quality, where growth rates at depressed temperatures (<20˚C) are higher than growth rates at ambient temperatures.
Psychrophilic, can only grow at depressed temperatures (<20˚C).
Allen, M. B. (1959). Studies with Cyanidium caldarium, an anomalously pigmented chlorophyte. Arch Mikrobiol 32, 270– 277. Table 2. Culture medium for Cyanidium caldarium Macroelements (NH4)2SO4, 0.01 M KH2PO4, 0.002 M MgSO4, 0.001 M CaCl2, 0.0005 M H2SO4, 0.001 M Microelements Fe, 4 mg/L Mn 0.5 mg/L B mg/L Zn mg/L Cu mg/L Mo mg/L V mg/L pH brought to 2 with sulfuric acid.
A mineral-salts, liquid microbiological culture medium containing sulfuric acid. For the autotrophic growth of Cyanidium.
From: Li-Jun Liu,3 Xiao-Yan You,3 Xu Guo, Shuang-Jiang Liu and Cheng-Ying Jiang. 2011. Metallosphaera cuprina sp. nov., an acidothermophilic, metal-mobilizing archaeon. IJSEM 61:2395-2400. Allen medium modified by the addition of (l-1): 5 g elemental sulfur, 5 mmol K2S4O6, 10mmol Na2S2O3, 13.9 g FeSO4 . 7H2O, 5 g pyrite or 5 g chalcopyrite. K2S4O6, Na2S2O3 or FeSO4 were added as filter sterilized solutions to the sterilized basal Allen medium (121 ˚C for 20 min).
A mineral-salts, liquid microbiological culture medium containing sulfuric acid, ferrous sulfate, thiosulfate, elemental sulfur, tetrathionate, and a metal sulfide (pyrite or chalcopyrite). For the autotrophic growth of Cyanidium.
Temperature optimum quality, growth rates at moderate temperatures (20-40˚C) are higher than growth rates at other temperatures, but will tolerate lower temperatures.
A prokaryotic physiological quality relating the optimal temperature of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division.
A prokaryotic quality relating the optimal salinity of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division.
An organic-rich, liquid microbiological culture medium containing yeast extract and peptone in a PIPES buffered artificial seawater base. Prepared under an atmosphere of dinitrogen. Used for the cultivation of Palaeococcus helgesonii.
From: Jan P. Amend, D’Arcy R. Meyer-Dombard, Seema N. Sheth, Natalya Zolotova, Andrea C. Amend. Palaeococcus helgesonii sp. nov., a facultatively anaerobic, hyperthermophilic archaeon from a geothermal well on Vulcano Island, Italy. Arch Microbiol (2003) 179 : 394–401. contains (per l of water): 28.22 g NaCl, 11.53 g MgCl2·6H2O, 1.56 g MgSO4·7H2O, 0.70 g KCl, 0.20 g NaHCO3. 3 g yeast extract, 3 g peptone, 10 ml concentrated N/P solution (containing per l of water: 127.51 g NH4Cl, 60.50 g NaNO3, 4.61 g K2HPO4), 0.5 ml of a 2% resazurin solution as an oxygen indicator, 5 g of PIPES (piperazine-N,N′-bis[2-ethanesulfonic acid]) as pH buffer. The pH was adjusted to 6.5 with 1.0 N NaOH, and the medium was then autoclaved at 121 °C for 20 min. Subsequently, the following filtersterilized (0.2 μm) solutions were added: 10 ml trace element solution/l (containing per l of water: 11.424 g KBr, 1.024 g AlK(SO4)2·12H2O, 0.423 g MnSO4·H2O, 12.0 mg BaCl2·2H2O, 0.5 mg CoCl2·6H2O, 42.4 mg CuSO4·5H2O, 0.5 mg H2WO4, 0.4 mg Na2MoO4·2H2O, 15.8 mg NiCl2·6H2O, 8.5 mg VOSO4·3.5H2O, 36.5 mg ZnSO4·7H2O, 0.4 mg CdSO4·8/3H2O, 1.8 mg PbCrO4, 66.0 mg RbCl, 5.0 mg SrCl2·6H2O), 5 ml/l CaCl2 solution (containing per l of water: 13.25 g CaCl2·2H2O), and 2ml/l Fe-EDTA solution (containing per l of water: 1.54 g FeSO4·7H2O and 2.06 g Na2EDTA). The YPS-V medium was then heated to boiling and degassed under a steady stream of N2. Ten ml of anoxic medium were transferred to acid-washed Balch tubes (25 ml) containing ~0.3 g S˚ (sterilized by heating to 98 °C in an oven for at least 3 days with occasional stirring) under N2. Tubes were capped with butyl-rubber stoppers and Al crimp seals and pressurized with N2 to 3 bar. Prior to inoculation, growth tubes were reduced with 0.15 ml of a 2.5% Na2S solution.
Salinity optimum quality, where growth rates at elevated salt concentrations are higher than growth rates at lower salt concentrations.
Halophilic, where growth occurs only at elevated salt concentrations.
Salinity optimum quality, where some growth occurs at elevated salinity, but growth is fastest at lower salinity.
A prokaryotic physiological quality relating the optimal pressure of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division.
From: Brock TD, Brock KM, Belly RT, Weiss RL. 1972. Sulfolobus: A new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Mikrobiol 84:54-68. Culture Media. For most studies a basal salts medium modified from Allen (1959) was used. This medium contains (NH4)2SO4, 1.3g KH2PO4, 0.28 g MgSO4x7H2O, 0.25g CaCl2x2H2O, 0.07 g FeCl3x6H2O, 0.02g MnCl2x4H2O, 1.8 mg Na2B4O7x10H2O, 0.03 mg VOSO4x2H2O, 0.03 mg CoSO4, 0.01 mg The pH was adjusted with 10 N H2SO4 to pH 1 or 2. Organic supplements were added to the basal medim either as dry powder before autoclaving or from 100-fold concentrated sterile stock solutions (for sugars) after autoclaving. Elemental sulfur was sterilized by steaming in the dry state for 3 h on each of 3 successive days and added at a concentration of about 1 g/100 ml to sterile media.
A mineral-salts, liquid microbiological culture medium containing sulfuric acid, elemental sulfur, and cobalt sulfate. For the growth of Sulfolobus spp.
Psychrophilic, can only grow at moderate temperatures (20-45˚C).
A prokaryotic size assay which uses a Berkefeld water filter with a particular pore size to determine the size of a prokaryotic cell.
A microbiological diagnostic assay which uses a water filter with a particular pore size to determine the size of a prokaryotic cell.
A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.45 microns in diameter.
A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with a narrow pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms.
Allen's medium supplemented with glucose and yeast extract
From: Ren-Long Jan, Jeffrey Wu, Shu-Miaw Chaw, Chien-Wei Tsai and Suh-Der Tsen. 1999. A novel species of thermoacidophilic archaeon, Sulfolobus yangmingensis sp. nov. IJSB 49:1809-1816. .....modified Allen's medium supplemented with glucose and yeast extract under aerobic conditions at low pH....
A mineral-salts, liquid microbiological culture medium containing sulfuric acid, glucose, and yeast extract. For the growth of Sulfolobus yangmingensis.
A hypersaline, solid microbiological culture medium containing mineral-salts, magnesium sulfate, pyruvate, glucose, glutamate, citrate, and peptones. Used for the cultivation of Halogranum rubrum.
A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, magnesium chloride and magnesium sulfate, sodium bromide, and yeast extract. Used for the cultivation of Halorubrum aquaticum.
From: M. C. Gutierrez, A. M. Castillo, P. Corral, M. Kamekura and A. Ventosa. 2011. Halorubrum aquaticum sp. nov., an archaeon isolated from hypersaline lakes. IJSEM 61:1144-1148. Water samples were plated on agar plates of halophilic medium (MH), containing (per litre distilled water) 195 g NaCl, 32.5 g MgCl2 . 6H2O, 50.8 g MgSO4 . 7H2O, 0.8 g CaCl2, 5 g KCl, 0.16 g NaHCO3, 0.6 g NaBr, 5 g yeast extract 20 g agar (pH 7.5)
From: SUSUMU ASAKAWA, HIROYUKI MORII, MASAYO AKAGAWA-MATSUSHITA, YOSUKE KOGA, AND KOICHI HAYANO. 1993. Characterization of Methanobrevibacter arboriphilicus SA Isolated from a Paddy Field Soil and DNA-DNA Hybridization among M. arboriphilicus strains. IJSB 43(4):683-686. The basal medium used for enrichment and isolation of strain SA was a modification of LPBM (22), which contained (per liter of deionized water) 0.75 g of KH2P04, 0.75 g of K2HP04, 1.0 g of NH4Cl, 0.36 g of MgCl2.6H20, 9 ml of a trace mineral solution (13), 10 ml of a vitamin mixture solution (2), 0.5 ml of a 0.2% resazurin solution, 0.5 g of L-cysteine hydrochloride H2O, 0.5 g of Na2S.9H20, and 4.8 g of NaHCO3. For cultivation of Methanobrevibacter arboriphilicus strains, the basal medium was supplemented with 0.1% yeast extract (Difco). For roll tubes, we added 0.1% yeast extract (Difco), 0.1% Polypeptone (Daigo Eiyo Co., Osaka, Japan), and 1.7% agar to the basal medium. The gas phase was H2-C02 (4:l) pressurized to 203 kPa, and the pH was 7.0. ******* From: Hiroyuki Morii, Masateru Nishihara and Yosuke Koga. 1983. Isolation, characterization and physiology of a new formate-assimilable methanogenic strain (A2) of Methanobrevibacter arboriphilus. Agric. Biol. Chem. 47(12):2781-2789. The trace mineral solution was as described by Zeikus(4) except that 0.03 g/liter NiCl2 was included.
An organic-rich, liquid, anaerobic microbiological culture medium containing L-cysteine, mineral-salts, yeast extract, resazurin, sulfide, sodium bicarbonate, carbon dioxide, and H2 gas as a substrate for methanogenesis. Used for the cultivation of Methanobrevibacter arboriphilicus. Modified by the addition of nickel (in the trace element solution).
A trace elements solution containing potassium hydroxide, nitrilotriacetic acid, ferrous chloride, manganese chloride, cobalt chloride, zinc chloride, calcium chloride, boric acid, and sodium molybdate.
From: Zeikus JG. 1977. The biology of methanogenic bacteria. Bacteriol Rev 41:514. Table 4 Trace mineral solution Contains, in grams per liter of distilled water (pH to 7.0 with KOH): nitrilotriacetic acid, 4.5; FeCl2 *4H20, 0.4; MnCl2 * 4H20, 0.1; CoCl2 * 6H2O, 0.17; ZnCl2, 0.1; CaC12, 0.02; H3BO3, 0.019; and sodium molybdate, 0.01.
A Berkefeld filter assay that tests whether a prokaryotic cell is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.43 microns in diameter.
From: Zeikus JG. 1977. The biology of methanogenic bacteria. Bacteriol Rev 41:514. Table 4 Composition of basal medium (LPBM) used for selective enrichment and growth of some methanogenic species Component Amount KH2PO4 ........... ........... 0.75 g K2HPO4-3H2O ...................... 1.45 g NH4Cl ...................... 0.9 g MgCl2 H20 ................ ...... 0.2 g Na2S.9H2Ob ...................... 0.5 g Trace mineral solutionc ................. 9 ml Vitamin solutiond ................... 5 ml Resazurin solution (0.2%)e .............. 1 ml Distilled H20 ............ ....... 1,000 ml a Prepared anaerobically under a 95% N2-5% CO2 gas atmosphere. Medium adjusted to pH 7.4 prior to autoclaving. Basal medium requires the addition of an electron donor (H2, formate, or methanol) for enrichment or growth of methanogens. b Sulfide solution added after sterilization. e Contains, in grams per liter of distilled water (pH to 7.0 with KOH): nitrilotriacetic acid, 4.5; FeCl2 *4H20, 0.4; MnCl2 * 4H20, 0.1; CoCl2 * 6H2O, 0.17; ZnCl2, 0.1; CaC12, 0.02; H3BO3, 0.019; and sodium molybdate, 0.01. d Optional, some methanogens require a vitamin mixture (11, 113). e Optional, an oxidation-reduction indicator. ref 113: Wolin EA, Wolin MJ, Wolfe RS. 1963. Formation of methane by bacterial extracts. J Biol Chem 238(8):2882-2886.
An organic-rich, liquid, anaerobic microbiological culture medium containing mineral-salts, resazurin, sulfide, carbon dioxide, and either formate, methanol or H2 gas as a substrate for methanogenesis. Prepared under an atmosphere of dinitrogen and carbon dioxide. Used for the cultivation of Methanobrevibacter arboriphilicus.
A hypersaline, organic-rich, solid microbiological culture medium containing mineral-salts, magnesium sulfate, yeast extract and casamino acids, and sodium glutamate. Used to culture Halobacter spp.
From ATCC Medium: 2170 Halobacter Medium Yeast Extract……………………………….5.0 g Casamino acids…………………………...5.0 g Sodium Glutamate………………………..1.0 g KCl………………………………………….2.0 g Sodium citrate (tri-Na)…………………….3.0 g MgSO4 x 7H2O…………………………...20.0 g NaCl………………………………………..200.0 g FeCl2 x 4H2O…………………………….36.0 mg MnCl2 x 4H2O……………………………0.36 mg Agar……………………………………….20.0 g DI Water…………………………………..1000 ml Adjust pH to 7.0-7.2. Autoclave at 121ºC. Caution: Due to high salt concentration, agar may not gel firmly. If this happens, agar and salt may have to be sterilized separately and combined.
Zeikus trace mineral solution with nickel
Zeikus trace mineral solution with nickel chloride added.
A mineral-salts, liquid microbiological culture medium containing resazurin and acetate as a carbon source. Prepared under an atmosphere of carbon dioxide and dinitrogen. For the growth of Methanosaeta (formerly Methanothrix).
From: S. H. Zinder, T. Anguish, and A. L. Lobo. 1987. Isolation and characterization of a thermophilic acetotrophic strain of Methanothrix. Arch Microbiol 146:315-322. Unless stated otherwise, the culture medium contained (g/l): NH4C1, 0.5; K2HPO4, 0.4; MgCI2x 6H2O, 0.1; resazurin, 0.001 ; trace metal solution (Zeikus 1977 except that 0.02 g/1 NiCI2x6H2O was added), 10 ml/1. The medium was either boiled under N2 (scrubbed of O2 by hot copper coils) or bubbled 20 min with N2 to make it anoxic, and was transferred into an anaerobic glove box (Coy Laboratory Products, Ann Arbor, MI, USA) where it was dispensed into 120 ml serum vials which were sealed with aluminum-crimp butyl rubber stoppers (Balch et al. 1979) purchased from Bellco Glass, Inc., Vineland, N J, USA. The vials were autoclaved at 121˚C for 20 minutes. The vial headspaces were flushed with sterile O2-scrubbed 70% N2/30% CO2 (Matheson Gas Products, Peoria, IL, USA) after which N2-flushed syringes were used to add the following sterile anoxic solutions to the following final concentrations: Na2S-9H20, 0.25 g/1 (ca. 1 mM); 2-mercaptoethane sulfonic acid, sodium salt (Sigma Chemical Corp., St. Louis, MO, USA), 1 mM; NaHCO3, 12 mM; CaCl2x2H2O, 0.1 g/l; sodium acetate, 40 mM; vitamin solution (Balch et al. 1979), 0.5ml/50ml. Cultures were routinely incubated at 60˚C, and the pH of the medium at 60˚C was 6.5.
A mineral-salts, liquid microbiological culture medium. Used to support the autotrophic growth of Cyanobacteria.
pH of the medium is 6.6 according to Algal Culturing Techniques, p. 437, copyright 2005.
http://www-cyanosite.bio.purdue.edu/media/table/bb.html NaNO3 (5.0 g/200 ml) 10.0 ml (~ 0.25 g) MgSO4·7H2O (1.5 g/200 ml) 10.0 ml (~ 0.75 g) NaCl (0.5 g/200 ml) 10.0 ml (~ 0.025 g) K2HPO4 (1.5 g/200 ml) 10.0 ml (~ 0.075 g) KH2PO4 (3.5 g/200 ml) 10.0 ml (~ 0.175 g) CaCl2·2H2O (0.5 g/200 ml) 10.0 ml (~ 0.025g) H3BO3 (1.14 g/100 ml) 1.0 ml (~ 0.114g) Trace elements solution 1.0 ml EDTA stock 1.0 ml Fe solution 1.0 ml Distilled water to 1.0 L For agar, add 15.0 g/L Bacteriological Agar* (Oxoid L11). Autoclave at 15 psi for 15 minutes. *Supplier: Unipath Ltd, Wade Road, Basingstoke, Hants RG24 0PW, UK Trace elements solution: ZnSO4·7H2O 8.82 g MnCl2·4H2O 1.44 g MoO3 0.71 g CuSO4·5H2O 1.57 g Co(NO3)2·6H2O 0.49 g Distilled water to 1.0 L May need autoclaving to dissolve. EDTA stock: EDTANa2 5.0 g KOH 3.1 g Distilled water to 100 ml Fe solution: FeSO4·7H2O 4.98 g conc H2SO4 1.0 ml Distilled water to 1.0 L
According to SCCAP the medium should have a final pH of 6-7. http://www.sccap.dk/media/freshwater/7.asp
A dilute, mineral-salts, liquid microbiological medium comprised of magnesium sulfate, sodium nitrate, calcium nitrate, ammonium chloride, sodium carbonate, ferric chloride, disodium EDTA, and micronutrients. Used for the cultivation of autotrophic Cyanobacteria.
http://www-cyanosite.bio.purdue.edu/media/table/Z8.html MgSO4·7H2O 0.25 g NaNO3 0.467 g Ca(NO3)2·4H2O 59 mg NH4Cl 31 mg Na2CO3 0.02 g FeEDTA solution 10 ml Gaffron micronutrients 1.0 ml Deionized water to 1.0 L FeEDTA solution: Made in two solutions: Solution A - 2.8 g FeCl3 in 100 ml 0.1 N HCl Solution B - 3.9 g EDTANa2 in 100 ml 0.1 N NaOH Add 10 ml solution A and 9.5 ml solution B plus water to 1 L. Gaffron micronutrients: H3BO3 3.1 g MnSO4·4H2O 2.23 g ZnSO4·7H2O 0.22 g (NH4)6Mo7O24·4H2O 0.088 g Co(NO3)2·6H2O 0.146 g VOSO4·6H2O 0.054 g Al2(SO4)3K2SO4·2H2O 0.474 g NiSO4(NH4)2SO4·6H2O 0.198 g Cd(NO3)2·4H2O 0.154 g Cr(NO3)3·7H2O 0.037 g Na2WO4·2H2O 0.033 g KBr 0.119 g KI 0.083 g Deionized water to 1 L
http://www-cyanosite.bio.purdue.edu/media/table/Z8.html Gaffron micronutrients: H3BO3 3.1 g MnSO4·4H2O 2.23 g ZnSO4·7H2O 0.22 g (NH4)6Mo7O24·4H2O 0.088 g Co(NO3)2·6H2O 0.146 g VOSO4·6H2O 0.054 g Al2(SO4)3K2SO4·2H2O 0.474 g NiSO4(NH4)2SO4·6H2O 0.198 g Cd(NO3)2·4H2O 0.154 g Cr(NO3)3·7H2O 0.037 g Na2WO4·2H2O 0.033 g KBr 0.119 g KI 0.083 g Deionized water to 1 L
A trace elements solution containing boric acid, manganese sulfate, zinc sulfate, ammonium molybdate, cobalt nitrate, vanadyl sulfate, nickel ammonium sulfate, cadmium nitrate, chromium nitrate, sodium tungstate, potassium bromide, and potassium iodide.
Single-organism metabolic process, where the prokaryotic microorganism uses enzymes to synthesize a storage molecule (usually a polymer) inside the cell that is used to store phosphorus.
prokaryote specialized with respect to pressure
Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth pressure.
prokaryote specialized with respect to salinity
Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth salinity.
prokaryote specialized with respect to temperature
Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth temperature.
http://www.nccs.res.in/mcc/Medium_64a.html TSBA100 TSB (Medium No. 64) 1.0 ml Distilled water 100 ml Agar 1.50 g
Tryptic soy broth diluted 100 times with water, solidified with agar added.
http://www.nccs.res.in/mcc/Medium_64a.html TSB100 TSB (Medium No. 64) 1.0 ml Distilled water 100 ml
Tryptic soy both diluted 100 times with water.
A liquid, mineral-salts microbiological culture medium. Used to support the autotrophic growth of cyanobacteria.
http://www-cyanosite.bio.purdue.edu/media/table/BG11.html NaNO3 1.5 g K2HPO4 0.04 g MgSO4·7H2O 0.075 g CaCl2·2H2O 0.036 g Citric acid 0.006 g Ferric ammonium citrate 0.006 g EDTA (disodium salt) 0.001 g Na2CO3 0.02 g Trace metal mix A5 1.0 ml Agar (if needed) 10.0 g Distilled water 1.0 L The pH should be 7.1 after sterilization Trace metal mix A5: H3BO3 2.86 g MnCl2·4H2O 1.81 g ZnSO4·7H2O 0.222 g NaMoO4·2H2O 0.39 g CuSO4·5H2O 0.079 g Co(NO3)2·6H2O 49.4 mg Distilled water 1.0 L
A trace elements solution containing boric acid, manganese chloride, zinc sulfate, sodium molybdate, copper sulfate, and cobalt nitrate.
http://www-cyanosite.bio.purdue.edu/media/table/BG11.html Trace metal mix A5: H3BO3 2.86 g MnCl2·4H2O 1.81 g ZnSO4·7H2O 0.222 g NaMoO4·2H2O 0.39 g CuSO4·5H2O 0.079 g Co(NO3)2·6H2O 49.4 mg Distilled water 1.0 L
An organic-rich, solid microbiological culture medium containing L-cysteine, D-glucose, citrate, yeast extract, a pH buffer and a pH indicator.
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/3/c7595dat.pdf Product Description Christensen Citrate Agar is used for the differentiation of enteric pathogens and coliforms on the basis of citrate utilization. Organisms that metabolize citrate as a sole source of carbon cleave citrate to oxaloacetate and acetate via the citritase enzyme. Another enzyme, oxaloacetate decarboxylase, then converts oxaloacetate to pyruvate and CO2 . The CO2 combines with sodium and water to form sodium carbonate, an alkaline compound. As a result, the pH of the medium rises and the phenol red indicator changes from orange red to cerise. The yeast extract provides the necessary vitamins for the growth of the microorganisms. L- cysteine hydrochloride functions as a reducing agent. Dextrose is the fermentable carbohydrate. Sodium citrate is the energy source for the citrate utilizing organisms. Care should be taken while inoculating, as a too heavy inoculum may give a false positive result (4). Components Item g/L Yeast Extract 0.50 L-Cysteine Hydrochloride 0.10 Sodium Citrate 3.00 Dextrose 0.20 Monopotassium Phosphate 1.00 Sodium Chloride 5.00 Phenol Red 0.012 Agar 15.00 Final pH (at 25°C) 6.9 ± 0.2
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Fluka/Datasheet/11012dat.pdf 11012 Cetrimide Nalidixic acid Agar For the isolation and enumeration (MPN method) of Pseudomonas aeruginosa from food products. Recommended by the "Schweizerisches Lebensmittelbuch" 2001., chapter 56A. Composition: Gelatine peptone, pancreatic 16.0 Casein hydrolysate 10.0 Potassium sulfate 10.0 Magnesium chloride 1.4 Cetrimide 0.2 Agar 15.0 Final pH 7.3+/- 0.2 at 37 °C Directions: Dissolve 52.6 g in 950 ml distilled water, add 10 ml glycerol (Fluka 49769) and sterilize by autoclaving at 121°C for 15 minutes. After cooling to 45-50°C add the rehydrate content of 1 vial Cetrimide Nalidixic acid Agar Supplement (Fluka 50225). Mix well. The prepared agar may contain slight precipitates. Principle and Interpretation: Gelatine peptone and Casein hydrolysate provides amino acids and other complex nitrogenous substances. Cetrimide (Cetyltrimethylammonium bromide; Fluka 52370) is incorporated in the medium to inhibit bacteria other than Pseudomonas aeruginosa. lt acts as a quaternary ammonium compound and cationic detergent which causes nitrogen and phosphorus to be released from bacterial cells other than Pseudomonas aeruginosa. Nalidixic acid present in the supplement improve the inhibition of the accompanying microbial flora (3, 4). For the isolation of Pseudomonas aeruginosa, plates of Cetrimide Agar should be inoculated from non-selective medium such as Brain Heart Infusion Broth (Fluka 70138) or Tryptone Soya Broth (Fluka 22092). If the count is high the test sample can be directly inoculated onto this medium. According the Schweizerisches Lebensmittelbuch (2) it is recommended to to put membrane filter, with different dilutions, directly on the agar plates. Glycerol is the carbon source. Magnesium chloride and potassium sulfate enhance the production pycocyanin and fluorescein. Pseudomonas aeruginosa colonies appear pigmented blue, bluegreen, red or nonpigmented but are fluorescent under UV light (360nm).
An organic-rich, solid microbiological culture medium that contains cetrimide and nalidixic acid.
http://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&ved=0CEEQFjAF&url=http%3A%2F%2Fwww.bd.com%2Feurope%2Fregulatory%2FAssets%2FIFU%2FHB%2FCE%2FBA%2FBA-257165.pdf&ei=L0_ZVJ6KCs-ryATu2YL4DQ&usg=AFQjCNH2fsKGxD4irxeJNurPKjLHCctpjA&sig2=T-2Ahw_G8MVq6GlpMrCnwQ&bvm=bv.85464276,d.aWw INTENDED USE BD Dorset Egg Medium, Modified is a prepared tubed, slanted, coagulated egg medium used for cultivation, maintenance and transport of pure cultures of mycobacteria and other fastidious and nonfastidious organisms. PRINCIPLES AND EXPLANATION OF THE PROCEDURE Microbiological method. BD Dorset Egg Medium, Modified is a modification of the whole egg medium described by Dorset.1 It is a nonselective medium well suited for the growth and maintenance of pure cultures of mycobacteria.2 More recently, it has also been used for the maintenance and transport of other bacterial species, such as Streptococcus pneumoniae, Neisseria meningitidis, Haemophilus influenzae, and enterotoxigenic E. coli at ambient temperature.3-5 Beef extract and peptone provide nutrients such as amino acids and organic phosphates. Whole egg mass contains complex nutrients necessary for bacterial and mycobacterial growth and, additionally, neutralizes toxic compounds. The inspissation process during the preparation of the medium provides the necessary solidity of the medium and inactivates bactericidal compounds contained in eggs such as lysozyme. REAGENTS BD Dorset Egg Medium, Modified Formula* Per Liter Purified Water Beef Extract 3.0 Peptone 5.0 Egg Mass (from whole fresh eggs) 750 ml pH 7.2 +/- 0.3
An organic-rich, liquid microbiological culture medium containing peptone, beef extract, and coagulated egg. Used to grow Mycobacteria.
The homogenized whole egg of a chicken that has been coagulated by the process of inspissation (drying), used in the cultivation of microorganisms.
A minerals-salts, solid culture medium containing ammonium nitrate, calcium chloride, magnesium sulfate, potassium phosphate, and potassium nitrate as major salts. Used for the cultivation of plant cells (for plant cell culture).
http://en.wikipedia.org/wiki/Murashige_and_Skoog_medium Murashige and Skoog medium or (MSO or MS0 (MS-zero)) is a plant growth medium used in the laboratories for cultivation of plant cell culture. MSO was invented by plant scientists Toshio Murashige and Folke K. Skoog in 1962 during Murashige's search for a new plant growth regulator. A number behind the letters MS is used to indicate the sucrose concentration of the medium. For example MS0 contains no sucrose and MS20 contains 20 g/l sucrose. Along with its modifications, it is the most commonly used medium in plant tissue culture experiments in laboratorium.[1] Ingredients Major salts (macronutrients) Ammonium nitrate (NH4NO3) 1,650 mg/l Calcium chloride (CaCl2 · 2H2O) 440 mg/l Magnesium sulphate (MgSO4 · 7H2O) 370 mg/l Potassium phosphate (KH2PO4) 170 mg/l Potassium nitrate (KNO3) 1,900 mg/l Minor salts (micronutrients) Boric acid (H3BO3) 6.2 mg/l Cobalt chloride (CoCl2 · 6H2O) 0.025 mg/l Cupric sulphate (CuSO4 · 5H2O) 0.025 mg/l Ferrous sulphate (FeSO4 · 7H2O) 27.8 mg/l Manganese sulphate (MnSO4 · 4H2O) 22.3 mg/l Potassium iodide (KI) 0.83 mg/l Sodium molybdate (Na2MoO4 · 2H2O) 0.25 mg/l Zinc sulphate (ZnSO4·7H2O) 8.6 mg/l Na2EDTA · 2H2O 37.2 mg/l Vitamins and organics i-Inositol 100 mg/l Niacin 0.5 mg/l Pyridoxine · HCl 0.5 mg/l Thiamine · HCl 0.1 mg/l IAA 1–30 mg/l Kinetin 0.04–10 mg/l Glycine (recrystallized) 2.0 mg/l Edamin (lactalbumin hydrolysate) 1.0 g/l Agar 10 g/l An optimum pH of 5.8 should be maintained.
An organic-rich, liquid microbiological culture medium containing L-cysteine, D-glucose, starch, the extracts of liver, beef and yeast, peptones, digested serum, and sodium thioglycollate. Thioglycollate and L-cysteine are reducing agents to provide anaerobiosis.
http://www.himedialabs.com/TD/M1801.pdf Ingredients Gms / Litre Peptic digest of animal tissue 10.000 Papaic digest of soyabean meal 3.000 Proteose peptone 10.000 Digested serum 13.500 Yeast extract 5.000 Beef extract 2.200 Liver extract 1.200 Dextrose 3.000 Potassium dihydrogen phosphate 2.500 Sodium chloride 3.000 Starch, Soluble 5.000 L-Cysteine hydrochloride 0.300 Sodium thioglycollate 0.300 Final pH ( at 25°C) 7.3±0.1 Principle And Interpretation Gifu Anaerobic Medium (GAM Broth) is a liquid medium for anaerobic bacteria. As this medium contains the digested serum which has hemin, it is successfully used for cultivation of anaerobic organisms such as streptococci, pneumonococci and meningococci. This medium is also suitable for blood culture (1). Anaerobic organisms require reducing condition and an absence of dissolved oxygen in the medium. Strict anaerobes obtain its energy and intermediates through oxidation utilizing hydrogen acceptors other than oxygen. Pre-reducing the medium by boiling to drive off the oxygen can expel this (2). Sodium thioglycollate and L-Cysteine are the reducing agents added in this medium to provide adequate anaerobiosis. Anaerobic bacteria vary in their sensitivy to oxygen and nutritional requirements (3). Peptic digest of animal tissue and yeast extract provide nitrogen, carbon and vitamin source. Starch absorbs the toxic metabolites produced (4). Hemin serves as essential growth factor and Sodium chloride maintains osmotic equilibrium (5).
From: Moeller KCN Broth Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Moeller KCN Broth Base, when supplemented with a solution of potassium cyanide, is used in the differentiation of enteric bacilli on the basis of their ability to grow promptly in the presence of cyanide. Principles of the Procedure The addition of 0.15 mL of a 0.5% solution of potassium cyanide to each of the prepared tubes of the nutritive base enables differentiation of members of various genera within the Enterobacteriaceae family. Moeller KCN Broth Base Approximate Formula* Per Liter Purified Water Pancreatic Digest of Casein ........................................1.5 g Peptic Digest of Animal Tissue ..................................1.5 g Sodium Chloride ..........................................................5.0 g Monopotassium Phosphate ........................................0.225 g Disodium Phosphate ....................................................5.64 g pH 7.6
An organic-rich, liquid culture medium comprised of polypeptone, yeast extract, sodium chloride, magnesium chloride, calcium chloride, sodium molybdate, copper chloride, and ferric chloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1071.pdf 1071. PY-BROTH Polypepton 2.000 g Bacto yeast extract (Difco) 0.500 g NaCl 30.000 g MgCl2 x 6 H2O 5.000 g CaCl2 x 2 H2O 0.005 g Na2MoO4 x 7 H2O 0.005 g CuCl2 x 2 H2O 0.004 g FeCl3 x 6 H2O 0.006 g Agar 15.000 g Distilled water 1000.000 ml pH 8.0 © 2007 DSMZ GmbH - All rights reserved
From: Eugon Agar (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eugon Agar is a general-purpose medium used for cultivating a wide variety of microorganisms. Principles of the Procedure Peptones provide the nitrogen, vitamins and amino acids in Eugon Agar. The high concentration of dextrose is the energy source for rapid growth of bacteria. L-Cystine and sodium sulfite are added to stimulate growth. Sodium chloride maintains the osmotic balance of the media. The high carbohydrate content along with high sulfur (cystine) content improves growth with chromogenicity.2 Agar is the solidifying agent in Eugon Agar. Formula Difco™ Eugon Agar Approximate Formula* Per Liter Proteose Peptone No. 3................................................ 7.5 g Pancreatic Digest of Casein.......................................... 7.5 g Soy Peptone................................................................. 5.0 g Dextrose...................................................................... 5.5 g L-Cystine...................................................................... 0.7 g Sodium Chloride.......................................................... 4.0 g Sodium Sulfite.............................................................. 0.2 g Agar.......................................................................... 15.0 g pH 7.0
An organic-rich, solid microbiological culture medium containing L-cystine, D-glucose, peptones, and sodium sulfite.
Prokaryote specialized with respect to pressure, which grows optimally at elevated pressures.
An organic-rich, liquid microbiological culture medium containing L-cystine, D-glucose, peptones, and sodium sulfite.
From: Eugon Broth (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Eugon Broth (Eugonbroth™) is a general-purpose medium used for the cultivation of fastidious and nonfastidious bacteria from a variety of clinical and nonclinical specimens. Principles of the Procedure Peptones supply amino acids and other nitrogenous substances to support bacterial growth. L-cystine is an essential amino acid that improves growth. Dextrose is incorporated as a source of energy and sodium chloride provides osmotic equilibrium. Sodium sulfite along with the cystine content improves growth with chromogenicity. Formula Bacto™ Eugon Broth Approximate Formula* Per Liter Proteose Peptone No. 3................................................ 7.5 g Pancreatic Digest of Casein.......................................... 7.5 g Soy Peptone................................................................. 5.0 g Dextrose...................................................................... 5.5 g L-Cystine...................................................................... 0.7 g Sodium Chloride.......................................................... 4.0 g Sodium Sulfite.............................................................. 0.2 g pH 7.0
A barophile, which grows only at elevated pressures.
Microbiological medium ingredient, derived from the acid hydrolysis of proteins in (blood) serum.
An aqueous (water) extract of the dried liver of a mammal (typically a cow), used in the cultivation of microorganisms.
http://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=13 To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
Prokaryote specialized with respect to pressure, which grows at elevated pressures and at atmospheric pressure.
An enzymatic hydrolysate of lactalbumin (a milk protein from cow's milk, Bos taurus) made using hydrolytic enzymes, used for the culturing of microorganisms.
http://www.neogen.com/Acumedia/pdf/ProdInfo/7465_PI.pdf Intended Use Lactalbumin Hydrolysate is an enzymatic digest of lactalbumin for use in preparing microbiological culture media. Principles of the Procedure Lactalbumin Hydrolysate provides nitrogen, amino acids, vitamins, and carbon in microbiological culture media.
Microbiological medium ingredient, derived from enzymatic hydrolysis of milk protein. An enzymatic hydrolysate of casein (a milk protein from cow's milk, Bos taurus), used for the culturing of microorganisms.
Prokaryote specialized with respect to salinity, which grows optimally at salinities of > 1.7%.
From: Hai-Qin Tan, Tian-Tian Li, Chu Zhu, Xin-Qi Zhang, Min Wu and Xu-Fen Zhu. 2012. Parabacteroides chartae sp. nov., an obligately anaerobic species from wastewater of a paper mill. IJSEM 62:2613-2617. The initial enrichment used medium Gs [containing l-1 distilled water: 10 g NaCl, 1.0 g MgCl2 . 6H2O, 0.5 g K2HPO4, 0.7 g KH2PO4, 0.025 g FeSO4 . 7H2O, 0.2 g CaCl2 . 2H2O, 1.0 g urea, 5 g yeast extract (Difco), 5 g tryptone, 1 ml trace element solution SL-10 (Widdel et al., 1983), 0.4 g L-cysteine and 0.001 g resazurin
based on phosphate buffer pH table, the pH of this medium should be around 6.7. http://www.uslims.uthscsa.edu/po4buffers.php
An organic-rich, liquid microbiological culture medium containing urea, L-cysteine, resazurin, tryptone, and yeast extract. Used for the cultivation of Parabacteroides chartae.
A trace elements solution containing hydrochloric acid, ferrous chloride, cobalt chloride, manganese chloride, zinc chloride, boric acid, sodium molybdate, nickel chloride, and copper chloride.
From: ATCC Medium 2734 Trace element solution SL10 The components of the trace element solutions SL10 (Widdel et al. 1983) are added and dissolved in the order listed. 25% HCl……………………………………………….10 ml FeCl2x4H2O………………………………………….1.5 g CoCl2x6H2O………………………………………….190 mg MnCl2x4H2O………………………………………….100 mg ZnCl2…………………………………………………..70 mg H3BO3………………………………………………….6 mg Na2MoO4x2H2O……………………………………..36 mg NiCl2x6H2O…………………………………………..24 mg CuCl2x2H2O………………………………………….2 mg DI Water……………………………………………….1000 ml The trace element solution is autoclaved under air, in 25 ml aliquots in 50 ml screw-capped bottles. Large stocks do not need to be sterilized for storage.
microbiological medium ingredient, derived from aqueous extracts
Undefined mixture of complex organic compounds derived from animal, fish, plant materials or soil, produced by the process of aqueous extraction (extraction using water, such as hot water). Used in the cultivation of microorganisms.
Halophilies, which only growt at salinities of > 1.7%.
Prokaryote specialized with respect to salinity, which can grow at salinities of > 1.7% or at lower salinities.
Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C), but can tolerate higher temperatures.
An organic-rich, solid culture medium comprised of polypeptone, yeast extract, sodium chloride, magnesium chloride, calcium chloride, sodium molybdate, copper chloride, ferric chloride and agar.
mildly reducing microbiological culture medium
A microbiological culture medium containing mild reducing agents (sodium thioglycollate or L-cysteine) which maintain a moderately low redox and hence promote the growth of facultative anaerobes.
strongly reducing microbiological culture medium
A microbiological culture medium containing strong reducing agents (such as sodium sulfide) which maintain a very low redox and hence promote the growth of anaerobes and strict anaerobes.
Culture medium quality with respect to the concentration of hydrogen ions, on a logarithmic scale.
Culture medium where the pH value is below 4.0.
Culture medium where the pH value is below 6.5.
Culture medium where the pH value is between 6.5 and 7.5.
Culture medium where the pH value is above 7.5.
Culture medium where the pH value is above 10.0.
Strongly reducing conditions dominated by the presence of cysteine, glutathione, 2-mercaptoethanol, dithiothreitol, sodium sulfide (hydrogen sulfide), dithionite, or titanium citrate.
Weakly reducing conditions dominated by the presence of organosulfides or thiosulfate as the reducing agent, but not sodium sulfide or hydrogen sulfide. zzzz
Oxidizing conditions due to the presence of oxygen.
Prokaryote specialized with respect to pH, which grows optimally at extremely acidic pH values (pH <4).
Prokaryote specialized with respect to pH, which grows optimally at moderately acidic pH values (pH 4-6).
Prokaryote specialized with respect to pH, which grows optimally at extremely alkaline pH values (pH >10).
Prokaryote specialized with respect to pH, which grows optimally at moderately alkaline pH values (pH 8-10).
prokaryote specialized with respect to pH
Prokaryotic physiologically differentiated cell, where the cell is specialized with resect to its optimal growth pH.
oxidizing microbiological culture medium
A microbiological culture medium lacking reducing agents (such as thioglycollate, L-cysteine, or sodium sulfide) while also having oxygen present. Promotes the growth of facultative anaerobes and aerobes.
Culture medium quality with respect to the oxidation-reduction potential of the medium.
Hyperalcidophile, only grows below pH 4.
Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8).
Alkaliphile, only grows between pH 8-10.
Hyperalkaliphile, only grows above pH 10.
Culture medium where the pH value is between 8.5 and 10.
Culture medium where the pH value is between 7.5 and 8.5.
Culture medium where the pH value is between 5.5 and 6.5.
Culture medium where the pH value between 5.5 and 4.0.
strongly acidic microbiological culture medium
Microbiological culture medium with a pH value below 4.0.
moderately acidic microbiological culture medium
Microbiological culture medium that has a pH value between 5.5 and 4.0.
slightly acidic microbiological culture medium
Microbiological culture medium with a pH value between 5.5 and 6.5.
near neutral pH microbiological culture medium
Microbiological culture medium that has a pH value between 6.5 and 7.5.
slightly alkaline microbiological culture medium
Microbiological culture medium with a pH value between 7.5 and 8.5.
moderately alkaline microbiological culture medium
Microbiological culture medium with a pH value between 8.5 and 10.
strongly alkaline microbiological culture medium
Microbiological culture medium with a pH value above 10.0.
Microbiological culture medium that has a pH value less than 6.5.
Microbiological culture medium that has a pH value above 8.5.
A prokaryote which is capable of growth in the absence of oxygen. Can grow or live in the presence of oxygen.
Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C), but can tolerate lower temperatures.
microbiological assay of lysis of blood cells
Microbiological reactivity to lymphatic materials assay, where the assay is for the cytolysis (lysis) of blood cells.
microbiological assay of inhibition of blood clotting
A microbiological diagnostic assay that tests for the inhibition of blood clotting using blood plasma.
microbiological assay of promotion of blood clotting
A microbiological ractivity to lymphatic materials assay, which assays for the promotion of blood clotting.
microbiological assay of binding to blood cells
A microbiological reactivity to blood assay, to determine whether a microorganism causes blood cells to clump or a microorganism can adhere to blood cells.
A process of cytolysis, where a microorganism secretes a protein(s) which results in the formation of a pore in the membranes of erythrocytes (red blood cells) or leucocytes (white blood cells) and thus causing the cells to lyse.
Wikipedia:hemolysis_(microbiology) An assay for the capability of a microorganism to lyse red blood cells (hemolysis, haemolysis). Hemolysis is induced by the secretion of various hemolysins by particular pathogenic strains of bacteria. Hemolysins can be proteins or lipids which act by disrupting the membranes of red blood cells.
A process of hemolysis, where a protein secreted by a microorganism results in the formation of a pore in the membranes of erythrocytes (red blood cells) and thus causing the cells to lyse.
A process of hemolysis, where a protein (beta-hemolysin) secreted by a microorganism results in the hydrolysis of sphingomyelin in erythrocytes (red blood cells) and thus causing the cells to lyse.
A process of hemolysis, where a protein (gamma-hemolysin) secreted by a microorganism results in the formation of a pore in the membranes of leukocytes (white blood cells) and thus causing the cells to lyse.
Wikipedia: hemolysin γ-Hemolysin Unlike beta-hemolysin, it has a higher affinity for phosphocholines with short saturated acyl chains, especially if they have a conical form, whereas cylindrical lipids (e.g., sphingomyelin) hinder its activity. The lytic process, most commonly seen in leucocytes, is caused by pore formation induced by an oligomerized octamer that organizes in a ring structure. Once the prepore is formed, a more stable one ensues, named β-barrel. In this final part, the octamer binds with phosphatidylcholine.[8]
A hemolysin protein secreted by Staphylococcus aureus. Form pores in target blood cells.
From: Dalla Serra M, Coraiola M, Viero G, Comai M, Potrich C, Ferreras M, Baba-Moussa L, Colin DA, Menestrina G, Bhakdi S, Prévost G. 2005. Staphylococcus aureus bicomponent gamma-hemolysins, HlgA, HlgB, and HlgC, can form mixed pores containing all components. J Chem Inf Model 45(6):1539-45. Staphylococcal gamma-hemolysins are bicomponent toxins forming a protein family with leucocidins and alpha-toxin. Two active toxins (AB and CB) can be formed combining one of the class-S components, HlgA or HlgC, with the class-F component HlgB. These two gamma-hemolysins form pores with marked similarities to alpha-toxin in terms of conductance, nonlinearity of the current-voltage curve, and channel stability in the open state. AB and CB pores, however, are cation-selective, whereas alpha-toxin is anion-selective. gamma-Hemolysins' pores are hetero-oligomers formed by three or four copies of each component (indicated as 3A3B and 3C3B or 4A4B and 4C4B). Point mutants located on a beta-strand of the class-S component that forms part of the protomer-protomer contact region can prevent oligomer assembly. Interestingly, these mutants inhibit growth of pores formed not only by their natural components but also by nonstandard components. This lead to the hypothesis that mixed ABC pores could also be formed. By studying the conductance of pores, assembled in the presence of all three components (in different ratios), it was observed that the magnitudes expected for mixed pores were, indeed, present. We conclude that the gamma-hemolysin/leucocidin bicomponent toxin family may form a larger than expected number of active toxins by cross-combining various S and F components.
A hemolysin protein secreted by Group A Streptococci (Streptolysin O and Streptolysin S) which causes complete lysis of red blood cells, polymorphonuclear leukocytes (granulocytes), and lymphocytes. Also, a beta-hemolysin protein is secreted by Staphylococcus aureus which involves the hydrolysis of sphingomyelin, which causes cell death.
Wikipedia: Hemolysin β-hemolysin Upon investigating sheep erythrocytes, its toxic mechanism was discovered to be the hydrolysis of a specific membrane lipid, sphingomyelin, which accounts for 50% of the cell’s membrane. This degradation was followed by a noticeable rise of phosphoryl-choline due to the release of organic phosphorus from sphingomyelin and ultimately caused cell lysis.[7]
A hemolysin protein secreted by Staphylococcus aureus, which forms a pore in the membranes of human immune cells, in particular T-cells, monocytes, and peripheral blood lymphocytes. Pore formation results in apoptosis of the immune cell, resulting in cell lysis and death.
Wikipedia:Staphylococcus aureus alpha toxin Alpha-toxin, also known as alpha-hemolysin (Hla), is the major cytotoxic agent released by bacterium Staphylococcus aureus and the first identified member of the pore forming beta-barrel toxin family.[1] This toxin consists mostly of beta-sheets (68%) with only about 10% alpha-helices. The hla gene on the S. aureus chromosome encodes the 293 residue protein monomer, which forms heptameric units on the cellular membrane to form a complete beta-barrel pore. This structure allows the toxin to perform its major function, development of pores in the cellular membrane, eventually causing cell death.
Wikipedia: Hemolysin Hemolysins (UK spelling: haemolysins) are lipids and proteins that cause lysis of red blood cells by destroying their cell membrane. Although the lytic activity of some microbe-derived hemolysins on red blood cells may be of great importance for nutrient acquisition, many hemolysins produced by pathogens do not cause significant destruction of red blood cells during infection. Although hemolysins are capable of doing this for red blood cells in vitro. As mentioned above, most hemolysins are protein compounds, but others are lipids biosurfactants.[1]
Lipids or proteins that cause the lysis (cytolysis) of blood cells or immune cells.
An organic-rich, solid medium containing animal and plant peptones and sodium chloride. Supplemented with sheep blood.
A beta protein found in Streptococcus, acts with Streptolysin S to form cell pores which cause hemolysis.
A beta protein found in Streptococcus, acts with Streptolysin O to form cell pores which cause hemolysis.
Prokaryote specialized with respect to temperature, which grows optimally at moderate temperatures (20-45˚C).
A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgB and HlgC to form cell pores which cause hemolysis.
A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgA and HlgC to form cell pores which cause hemolysis.
A gamma-hemolysin protein found in Staphylococcus aureus, acts with HlgA and HlgB to form cell pores which cause hemolysis.
Electromagnetic radiation from the Sun that reaches the surface of the Earth.
Wikipedia: Light Light is electromagnetic radiation within a certain portion of the electromagnetic spectrum. The word usually refers to visible light, which is visible to the human eye and is responsible for the sense of sight.[1] Visible light is usually defined as having a wavelength in the range of 400 nanometres (nm), or 400×10−9 m, to 700 nanometres – between the infrared (with longer wavelengths) and the ultraviolet (with shorter wavelengths).[2][3] Often, infrared and ultraviolet are also called light. The main source of light on Earth is the Sun. Sunlight provides the energy that green plants use to create sugars mostly in the form of starches, which release energy into the living things that digest them. This process of photosynthesis provides virtually all the energy used by living things. Historically, another important source of light for humans has been fire, from ancient campfires to modern kerosene lamps. With the invention of electricity, electric lighting has all but replaced firelight. Some species of animals generate their own light, called bioluminescence. For example, fireflies use light to locate mates, and vampire squids use it to hide themselves from prey.
A prokaryotic metabolic process where the carbon or energy sources are organic compounds.
From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (ii) Osmotrophy: Cells take up organic material from the external environment as small molecules or macromolecules.
From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (i) Primary producer: Generates organic carbon by photosynthesis and CO2 fixation (the role traditionally played by phytoplankton).
A prokaryotic respiratory metabolic process where carbon dioxide is the source of carbon.
prokaryotic metabolically differentiated cell
A prokaryotic differentiated cell that carries out a specific metabolic role, and is therefore metabolically differentiated.
A prokaryotic cell, where the width of the cell is between 2 and 20 microns (um).
A prokaryote capable of carrying out the role of autotrophy, where carbon dioxide is the source of carbon.
Wikipedia: Autotroph An autotroph[α] ("self-feeding", from the Greek autos "self" and trophe "nourishing") or "producer", is an organism that produces complex organic compounds (such as carbohydrates, fats, and proteins) from simple substances present in its surroundings, generally using energy from light (photosynthesis) or inorganic chemical reactions (chemosynthesis). They are the producers in a food chain, such as plants on land or algae in water, in contrast to heterotrophs as consumers of autotrophs. They do not need a living energy or organic carbon source. Autotrophs can reduce carbon dioxide to make organic compounds for biosynthesis and also create a store of chemical energy. Most autotrophs use water as the reducing agent, but some can use other hydrogen compounds such as hydrogen sulfide. Phototrophs (green plants and algae), a type of autotroph, convert physical energy from sunlight into chemical energy in the form of reduced carbon.
A prokaryotic cell, where the width of the cell is between 0.2 and 2 microns (um).
A mesophile which can only grow at moderate temperatures (20-40˚C).
Prokaryote specialized with respect to temperature, which grows optimally at depressed temperatures (< 20˚C).
From: Worden AZ, Follows MJ, Giovannoni SJ, Wilken S, Zimmerman AE, Keeling PJ. 2015. Rethinking the marine carbon cycle: Factoring in the multifarious lifestyles of microbes. Science 347, DOI: 10.1126/science.1257594. (viii) Symbioses: Defined here as mutualistic relationships where one species lives on or within another species (Fig. 3 inset shows a diatom with N2-fixing cyanobacteria on its spines).
Wikipedia: Heterotroph A heterotroph (/ˈhɛtərɵtroʊf/; ἕτερος heteros = "another", "different" and τροφή trophe = "nutrition") is an organism that cannot fix carbon and uses organic carbon for growth.[1][2] Heterotrophs can be further divided based on how they obtain energy; if the heterotroph uses light for energy, then it is considered a photoheterotroph, while if the heterotroph uses chemical energy, it is considered a chemoheterotroph.
A prokaryote capable of carrying out the role of heterotrophy where the carbon or energy sources are organic compounds.
An environmental system occupied by a microorganism that floats in a column of water, where the organisms cannot actively swim against the current.
Wikipedia: Plankton Plankton (singular plankter) are a diverse group of organisms that live in the water column and cannot swim against a current.[1] They provide a crucial source of food to many large aquatic organisms, such as fish and whales. These organisms include drifting animals, protists, archaea, algae, or bacteria that inhabit the pelagic zone of oceans, seas, or bodies of fresh water; that is, plankton are defined by their ecological niche rather than phylogenetic or taxonomic classification. Though many planktonic species are microscopic in size, plankton includes organisms covering a wide range of sizes, including large organisms such as jellyfish.[2] The adjective planktonic is widely used in both the scientific and popular literature, and is a generally accepted term. However, from the standpoint of formal grammar the less commonly used planktic is more strictly the correct adjective. When deriving English words from their Greek or Latin roots the gender specific ending (in this case "-on," which indicates the word is neuter) is normally dropped, using only the root of the word in the derivation.[7]
A prokaryote capable of carrying out the role of phototrophy, where light is the energy source.
A prokaryotic respiratory metabolic process where the oxidation and reduction of inorganic chemical compounds is the energy source.
Wikipedia: Lithotroph Lithotrophs are a diverse group of organisms using inorganic substrate (usually of mineral origin) to obtain reducing equivalents for use in biosynthesis (e.g., carbon dioxide fixation) or energy conservation (i.e., ATP production) via aerobic or anaerobic respiration.[1] Known chemolithotrophs are exclusively microbes; no known macrofauna possesses the ability to utilize inorganic compounds as energy sources. Macrofauna and lithotrophs can form symbiotic relationships, in which case the lithotrophs are called "prokaryotic symbionts". An example of this is chemolithotrophic bacteria in giant tube worms or plastids, which are organelles within plant cells that may have evolved from photolithotrophic cyanobacteria-like organisms. Lithotrophs belong to either the domain Bacteria or the domain Archaea. The term "lithotroph" was created from the Greek terms 'lithos' (rock) and 'troph' (consumer), meaning "eaters of rock". Many lithoautotrophs are extremophiles, but this is not universally so. Different from a lithotroph is an organotroph, an organism which obtains its reducing agents from the catabolism of organic compounds.
A prokaryote capable of carrying out the role of mixotrophy, where phototrophy and heterotrophy co-occur at the same instance in time.
A prokaryote capable of carrying out the role of respiration, which involves the oxidation and reduction of chemical compounds and involves an electron transport chain.
A prokaryotic metabolic quality where carbon dioxide is the source of carbon.
A prokaryotic metabolic quality where light is the energy source, and the electron donor is an inorganic compound.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source.
A prokaryotic metabolic quality where the oxidation and reduction of inorganic chemical compounds is the energy source.
Crystalline cellulose comprised of microcrystalline or nanocrystalline cellulose fibers, commercially purified and prepared as filter paper.
A liquid minerals salt medium containing sodium nitrate, magnesium sulfate heptahydrate, potassium chloride, ferrous sulfate heptahydrate, dipotassium phosphate, potassium dibasic phosphate, yeast extract, and filter paper (a source of pure microcrystalline cellulose). Used for the cultivation of Sporocytophaga.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium167.pdf 167. SPOROCYTOPHAGA MEDIUM NaNO3 2.00 g MgSO4 x 7 H2O 1.00 g KCl 0.50 g FeSO4 x 7 H2O 6.00 mg KH2PO4 0.14 g K2HPO4 1.20 g Yeast extract 0.02 g Distilled water 1000.00 ml Adjust pH to 7.2. Place a strip of sterile filter paper into a culture tube containing 5 ml of medium, thereby leaving 1 - 2 cm of the strip outside of the medium. For DSM 11118 it is recommended to use the following modified medium for the reactivation of freeze-dried samples: Prepare agar plates of medium 167 by supplementing medium with 1.5% (w/v) agar. Place 4 pieces of sterile filter paper on each agar plate. Suspend freeze-dried content of the inner vial of one ampoule with 0.5 ml of liquid medium 167 and inoculate each piece of the filter paper with 1 large drop of the cell suspension. © 2007 DSMZ GmbH - All rights reserved
A prokaryotic metabolic quality where the carbon and energy sources are organic compounds.
A prokaryotic metabolic quality where phototrophy and heterotrophy co-occur at the same instance in time.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide.
A prokaryotic metabolic quality where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
A prokaryotic metabolic quality where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide
A prokaryotic metabolic quality where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryotic metabolic quality where light is the energy source, and the electron donor is an organic compound.
A prokaryotic metabolic quality where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide.
A prokaryotic metabolic quality where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
The metabolic quality of a prokaryotic microorganism.
A prokaryotic metabolic quality which involves the oxidation and reduction of chemical compounds and involves an electron transport chain.
A prokaryotic metabolic quality where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system.
A prokaryotic metabolic process that involves fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms.
A prokaryotic metabolic quality that involves fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms.
A prokaryotic metabolic quality that involves fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms.
A prokaryote capable of carrying out the role of chemotrophy, where the oxidation and reduction of chemical compounds is the energy source.
A prokaryote capable of carrying out the role of lithotrophy, where the oxidation and reduction of inorganic chemical compounds is the energy source.
A prokaryote capable of carrying out the role of fermentation, where the carbon and energy sources are organic compounds. Is an anaerobic process which does not require an electron transport system.
A prokaryote capable of carrying out the role of fermentation in the dark. Is carried out by photosynthetic prokaryotic microorganisms.
A prokaryote capable of carrying out the role of fermentation in the light. Is carried out by photosynthetic prokaryotic microorganisms.
A prokaryote capable of carrying out the role of chemolithotrophy, where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an inorganic compound.
A prokaryote capable of carrying out the role of chemolithoautotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide.
A prokaryote capable of carrying out the role of chemolithoheterotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryote capable of carrying out the role of chemoorganotrophy, where the oxidation and reduction of chemical compounds is the energy source, and the electron donor is an organic compound.
A prokaryote capable of carrying out the role of chemoorganoautotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is carbon dioxide.
A prokaryote capable of carrying out the role of chemoorganoheterotrophy, where the oxidation and reduction of chemical compounds is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
A prokaryote capable of carrying out the role of photolithotrophy, where light is the energy source, and the electron donor is an inorganic compound.
A prokaryote capable of carrying out the role of photolithoautotrophy, where light is the energy source, the electron donor is an inorganic compound, and the carbon source is carbon dioxide
A prokaryote capable of carrying out the role of photolithoorganotrophy, where light is the energy source, the electron donor is an inorganic compound, and the carbon source is an organic compound.
A prokaryote capable of carrying out the role of photoorganotrophy, where light is the energy source, and the electron donor is an organic compound.
A prokaryote capable of carrying out the role of photoorganoautotrophy, where light is the energy source, the electron donor is an organic compound, and the carbon source is an carbon dioxide.
A prokaryote capable of carrying out the role of photoorganoheterotrophy, where light is the energy source, the electron donor is an organic compound, and the carbon source is an organic compound.
A prokaryote which is either an aerobe, anaerobe, or facultative anaerobe.
A prokaryote which is capable of growth in the presence of oxygen.
A prokaryote which is capable of growth in the presence of oxygen, where the oxygen concentration is less than atmospheric levels.
A prokaryote which is capable of growth in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen).
A prokaryote which is capable of growth in the absence of oxygen.
A colony having distinct morphology that manifests in the appearance of the relative position of the parts of the colony.
A prokaryote which is capable of growth in the absence of oxygen. Cannot grow or live in the presence of oxygen.
A prokaryote which is capable of growth in the presence of oxygen or in the absence of oxygen.
A prokaryotic colony that has a distinct process quality characteristics.
A prokaryotic metabolic process where a microorganism grows (undergoes cell division) in the presence or absence of oxygen.
A prokaryotic metabolic process where the organism is capable of aerobic growth if oxygen is present and is capable of anaerobic growth if oxygen is absent.
A prokaryotic metabolic process where the organism grows in the absence of oxygen.
A prokaryotic metabolic process where an anaerobic microorganism can grow in the presence of oxygen, but cannot use oxygen as an energy source.
A prokaryotic metabolic process where the organism grows in the absence of oxygen. Cannot grow or live in the presence of oxygen.
A prokaryotic metabolic process where the organism grows in the presence of oxygen.
A prokaryotic metabolic process where the organism grows in the presence of oxygen. Is incapable of anaerobic growth (growth in the absence of oxygen). Uses only oxygen as a terminal electron acceptor.
A prokaryotic metabolic process where the organism grows in the presence of oxygen, where the oxygen concentration is less than atmospheric levels. Can use oxygen as a terminal electron acceptor through the process of aerobic respiration. Can also have anaerobic metabolic capabilities.
Quality of a microbiological culture medium.
zzzz *-7 and above labels have not been submitted to ChEBI as new terms yet. The numbers at the end of the temporary class labels help map entities in logical axioms, once the classes have been entered into ChEBI.
A psychrophile which can only grow at depressed temperatures (<20˚C).
Prokaryote specialized with respect to temperature, which grows optimally at elevated temperatures (45-85˚C).
Prokaryote specialized with respect to temperature, which grows optimally at elevated temperatures (>85˚C).
A thermophile which can only grow at elevated temperatures (45-85˚C).
A hyperthermophile which can only grow at elevated temperatures (>85˚C).
prokaryotic physiologically differentiated cell
Prokaryotic differentiated cell, where the cell is specialized to a particular physical condition with respect to temperature, salinity, or pressure.
The optimal growth temperature of a prokaryotic microorganism, which is the temperature at which it undergoes the most rapid pace of growth and cell division.
differentiated akinete position relative to heterocytes
Cyanobacterial filament where the position of akinetes is differentiated with respect to the physical position of heterocytes.
An assay for the ability of a microorganism to grow in the presence of a potentially growth inhibiting inorganic chemical.
A prokaryotic physiological quality relating the optimal pH of a particular strain of prokaryotes. When these preferred conditions are met, the organism will exhibit cell division.
pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other pH values.
pH optimum quality, where growth rates at moderately alkaline pH values (pH 8-10) are higher than growth rates at other pH values.
pH optimum quality, where growth rates at highly acidic pH values (pH < 4) are higher than growth rates at other temperatures.
pH optimum quality, where growth rates at moderately acidic pH values (pH 4-6) are higher than growth rates at other pH values.
pH optimum quality, where growth rates at highly alkaline pH values (pH >10) are higher than growth rates at other temperatures.
pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other temperatures, but will tolerate lower pH values.
pH optimum quality, where growth rates at circumneutral pH values (pH 6-8) are higher than growth rates at other temperatures, but will tolerate higher pH values.
Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8), but can tolerate lower pH values.
Prokaryote specialized with respect to pH, which grows optimally at circumneutral pH values (pH 6-8), but can tolerate higher pH values.
Neutrophilic, can only grow between pH 6-8.
From: Robert C, Bernalier-Donadille A. 2003. The cellulolytic microflora of the human colon: evidence of microcrystalline cellulose-degrading bacteria in methane-excreting subjects. FEMS Microbiology Ecology 46:81-89. Cellulolytic bacteria were enumerated in the basal cellulolytic (BC) medium containing, per litre: 200 ml clarified rumen fluid, 75 ml mineral solution I, 75 ml mineral solution II, 10 ml trace element solution [17], 10 ml vitamin solution [18], 10 ml volatile fatty acid solution [18], 0.5 g yeast extract, 1.0 g tryptone, 1 ml resazurin (0.1%), 1 ml hemin (0.1%), 20 ml cysteine sulfide-reducing agent, 2.5 g NaHCO3. Mineral solution I was composed of 6.0 g l31 K2HPO4 and mineral solution II contained, per litre, 6.0 g KH2PO4, 12 g (NH4)2SO4, 12.0 g NaCl, 1.2 g MgSO4, 1.2 g CaCl2. The cysteine sulfide-reducing agent contained 1.25% (w/v) each cysteine-HCl*H2O and Na2S*9H2O. A piece of Whatman No. 1 filter paper cellulose (50 mg) was added to each tube before the addition of the prereduced BC medium (10 ml per tube). After incubation at 37˚C for 21 days, cellulose utilisation in the culture tubes of each dilution inoculated (10^3-10^9) was detected visually by noting degradation of the filter paper. [17] Balch, W.E., Fox, G.E., Magrum, L.J., Woese, C.R. and Wolfe, R.S. (1979) Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43, 260-296. [18] Scott, H.W. and Dehority, B.A. (1965) Vitamin requirements of several cellulolytic bacteria. J. Bacteriol. 89, 1169-1175.
From: Scott HW, Dehority BA. 1965. Vitamin requirements of several cellulolytic rumen bacteria. J Bacteriol 89(5):1169-1175. Volatile Fatty Acids, from Table 1 (mg per 100 mL): Acetic acid 133.0 Isobutyric acid 6.6 Isovaleric acid 8.0 Valeric acid 8.0
A liquid, mineral-salts microbiological culture medium. Used to support the autotrophic growth of cellulolytic bacteria that also require rumen fluid. Contains rumen fluid, minerals salts, Balch trace element solution, vitamins, volatile fatty acids, yeast extract, tryptone, resazurin, hemin, cystine hydrochloride, sodium sulfide, sodium bicarbonate and filter paper (as a cource of cellulose).
From: Balch WE et al, 1979. Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260. 10 mL trace minerals (pH to 7.0 with KOH; per liter: nitrilotriacetic acid, 1.5 g; MgSO4x7H2O, 3.0 g; MnSO4x2H2O, 0.5 g; NaCl, 1.0 g; FeSO4x7H2O, 0.1g; CoSO4 or CoCl2, 0.1 g; CaCl2x2H2O, 0.1 g; ZnSO4, 0.1 g; CuSO4x5H2O, 0.01 g; AlK(SO4)2, 0.01 g; H3BO3, 0.01 g; Na2MoO4x2H2O, 0.01g); dissolve nitrilotriacetic acid with KOH to pH 6.5 then proceed to add minerals)
A trace elements solution containing KOH, nitrilotriacetic acid, magnesium sulfate heptahydrate, manganese sulfate dihydrate, sodium chloride, ferrous sulfate heptahydrate, cobalt sulfate (or cobalt chloride), calcium dichloride dihydrate, zinc sulfate, copper sulfate pentahydrate, aluminum potassium sulfate, boric acid, and disodium molybdate dihydrate.
A solid, organic rich culture medium based on trytic soy agar. Also contains additional yeast extract, potassium nitrate, sodium lactate, sodium succinate, sodium formate, hemin, menadione (vitamin K3), L-cysteine, dithiothreitol, glucose, sodium fumarate, sodium carbonate, and defibrinated sheep's blood. Prepared under an atmosphere of nitrogen, carbon dioxide, and hydrogen.
ATCC medium: 1257 ETSA medium Trypticase Soy Agar (BD 211043)...40.0 g Yeast extract......................1.0 g Agar...............................4.0 g KNO3 ...............................0.5 g Sodium lactate, 60% syrup..........1.3 ml Sodium succinate...................0.5 g Sodium formate.....................0.5 g Hemin Solution (see below).........1.0 ml Distilled water..................924.0 ml Autoclave the above solution at 121C for 15 minutes. Cool to 55C. Aseptically add the following, freshly prepared, filter-sterilized solutions in the order listed: Menadione Solution (see below).....2.0 ml 4% L-Cysteine . HCl...............10.0 ml 0.5% Dithiothreitol (DTT).........10.0 ml 10% Glucose.......................10.0 ml 1% Sodium fumarate.................2.0 ml 4% Na2CO3 .........................10.0 ml Defibrinated sheep blood..........30.0 ml This medium solidifies very quickly and should be maintained at 50-55C while dispensing. Aseptically tube the sterile completed medium under an anaerobic atmosphere of 80% N2, 10% CO2, 10% H2. Plug the tubes with butyl rubber stoppers. A note of caution: Hydrogen gas can be explosive in the concentration used in preparing this medium. Gas tanks should be equipped with spark arrestors. Hemin Solution: KOH................................1.12 g 95% Ethanol......................100.0 ml Hemin............................200.0 mg Distilled water..................100.0 ml Dissolve KOH in water. Add ethanol and hemin. Menadione Solution: Menadione (Vitamin K3)............50.0 mg 95% Ethanol.......................50.0 ml Distilled water...................50.0 ml Dissolve menadione in ethanol; then add water. Filter-sterilize solution.
The optimal growth salinity (osmolarity) of a prokaryotic microorganism, which is the salinity (osmolarity) at which it undergoes the most rapid pace of growth and cell division.
The optimal growth pH of a prokaryotic microorganism, which is the pH at which it undergoes the most rapid pace of growth and cell division.
Acialkaliphilic, can only grow between pH 8-10.
Acidophilic, can only grow between pH 4-6.
Hyperacialkaliphilic, can only grow above pH 10.
Hyperacidophilic, can only grow below pH 4.
From:https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Sheep Blood, Defibrinated is sheep blood that has been treated to denature fibrinogen without causing cell lysis. It has been shown to be the most accurate blood preparation for the determination of hemolytic activity of streptococci.(3) Sheep blood also contains enzymes that hydrolyze V-factor, thereby inhibiting growth of V-factor dependent Haemophilus haemolyticus which potentially could be misidentified as hemolytic streptococci.(3) Defibrinated sheep blood is free of anticoagulant or preservative. Because washed cell suspensions could result in fragile, hemolyzed blood cells, defibrinated sheep blood is not recommended as a source of washed cells.
From: http://medical-dictionary.thefreedictionary.com/defibrinated+blood defibrinated blood - whole blood from which fibrin has been separated during the clotting process.
Blood medium ingredient comprised of sheep's (Ovis aries) blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms.
A solid, organic rich microbiological medium for the growth of anaerobes. Contains peptone, yeast extract, sodium chloride, starch, dextrose (D-glucose), sodium pyruvate, arginine, sodium succinate, L-cysteine hydrochloride, sodium bicarbonate, ferric pyrophosphate, hemin, vitamin K, dithiothreitol, and agar.
From: http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0972 Typical Formula* gm/litre Peptone 16.0 Yeast extract 7.0 Sodium chloride 5.0 Starch 1.0 Dextrose 1.0 Sodium pyruvate 1.0 Arginine 1.0 Sodium succinate 0.5 L-cysteine HCl 0.25 Sodium bicarbonate 0.4 Ferric pyrophosphate 0.5 Haemin 0.005 Vitamin K 0.0005 Dithiothreitol 0.25 Agar 12.0 pH 6.8 ± 0.2
Blood medium ingredient comprised of horse's (Equus caballus) blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, Defibrinated is horse blood that has been treated to denature fibrinogen without causing cell lysis. It is used to supplement blood agar bases. It does not release V-factor hydrolyzing enzymes, and supports the growth of the hemolytic Haemophilus species. Defibrinated horse blood is free of anticoagulant or preservative. Because defibrinated horse blood may result in "incorrect" hemolytic reactions as compared to reactions on sheep blood media, definitive tests are recommended for the differentiation of group D streptococci from group A.(3)
From: http://www.hopebiol.com/medium/p13HB0398.htm Formula:(g/L) peptone 20.0g glucose 5.0g Baptist yeast powder 10.0g NaCl 0.08g Cysteine hydrochloride 0.5g Calcium chloride 0.008g MgSO4 0.008g K2HPO4 0.04g KH2PO4 0.04g sodium bicarbonate 0.4g PH 7.1-7.3
An organic-rich, liquid microbiological culture medium containing peptone, glucose, and yeast powder. Also contains minerals salts.
An undefined organic chemical mixture, derived from deactivated, dehydrated yeast (cells or Saccharomyces cerevisiae).
Wikipedia: Nutritional yeast Nutritional yeast is a deactivated yeast, often a strain of Saccharomyces cerevisiae, which is sold commercially as a food product. It is sold in the form of flakes or as a yellow powder and can be found in the bulk aisle of most natural food stores. It is popular with vegans and vegetarians and may be used as an ingredient in recipes or as a condiment.[1] Nutritional yeast is different from yeast extract, which has a very strong flavour and comes in the form of a dark brown paste.
alpha-aminobutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate alpha-aminobutyrate (alpha-aminobutyric acid) as a sole source of carbon and energy.
poly(ethylene glycol) assimilation assay
Assays for the ability of a microorganism to assimilate poly(ethylene) glycol as a sole source of carbon and energy.
An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent.
Assays for the ability of a microorganism to assimilate aconitic acid (aconitate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate trans-aconitate (trans-aconitic acid) as a sole source of carbon and energy.
An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-galactopyranoside. Beta-galactosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent.
6-phospho-beta-D-galactoside 6-phosphogalactohydrolase
A carbohydrate hydrolysis assay for the activity of 6-phospho-beta-galactosidase in a microorganism which carries out the following reaction: 6-phospho-beta-D-galactoside + H2O <=> 6-phospho-D-galactoside + an alcohol
6-phospho-beta-galactosidase assay with oNP
An assay for the activity of 6-phospho-beta-galactosidase in a microorganism. Uses the substrate 2-nitrophenyl-beta-D-galactopyranoside-6-phosphate. 6-phospho-beta-galactosidase (beta-galactosidase 6-phosphate) will cleave the substrate, producing 2-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An assay for the activity of alpha-galactosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-alpha-D-galactopyranoside. Alpha-galactosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent.
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate 4-methylumbelliferyl-beta-D-glucoside. Beta-glucosidase will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent.
Test for butyrate esterase activity in a microorganism using the substrate 4-methylumbelliferyl-butyrate. Esterase that can hydrolyze butyrate will cleave the substrate, producing 4-MU (4-methylumbelliferone), which is fluorescent. A positive result is fluorescent; a negative result is not fluorescent.
A carboxylic ester hydrolase activity, acting on buytrate derivatives.
A carbohydrate hydrolysis assay, specifically analyzing for the presence of hydrolases (such as lipases or esterases) in a microorganism.
A DNse assay with uses 5-bromo-4-chloro-3-indolyl-thymidine-3-phosphate as a substrate. DNase cleaves the substrate, producing 5-bromo-4-chloro-3-hydroxyindole, which is oxidized into 5,5'-dibromo-4,4'-dichloro-indigo, which is intensely blue. A positive result is intensely blue; a negative result is colorless.
An assay for the activity of DNase in a microorganism. Uses the substrate 4-nitrophenol-thymidine-5-monophosphate. DNase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
prokaryotic cultured clonal cell population
A cultured clonal cell population comprised of prokaryotic cells. Could be manifested as a colony or a liquid culture derived from a single individual (a homogenous population founded by a single individual, or clone).
An assay for bacterial hydrolytic assays which use indoxyl acetate as a substrate. In the presence of oxygen, released indoxyl will turn an indigo color indicating a positive reaction. Assays for the following reaction: Indoxyl acetate + H2O <=> Indoxyl + acetic acid
A test tube used to culture a microorganism.
A device used to culture microorganisms on solid microbiological culture medium.
A prokaryotic cultured clonal cell population housed in a test tube which uses a culture tube with liquid culture medium as a substrate to support growth.
RapID™ ANA II System, a set of specialized identification kits that contain 32 reagents designed to identify particular types of microorganisms.
Blood medium ingredient comprised of a peptic digest of sheep (Ovis aries) or horse (Equus caballus) blood, serves as a source of hemin and NAD (nicotinamide adenine dinucleotide). Used in the cultivation of microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Fildes Enrichment is a peptic digest of sheep or horse blood that serves as a rich source of growth factors including hemin (X-factor) and nicotinamide adenine dinucleotide (V-factor).
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemin Solution is a mixture of hemin and NaOH.
A defined organic solution comprised of a solution of hemin (derived from blood) and sodium hydroxide. Used in the cultivation of microorganisms.
A blood medium ingredient comprised of bovine (Bos taurus) hemoglobin that has been dried (i.e. has a decreased water content), providing a source of hemin. Used in the cultivation of microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemoglobin, Dried Bovine is used to provide hemin required by many fastidious microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Hemoglobin Solution, 2% provides hemin required by many fastidious microorganisms.
A defined organic solution comprised of hemoglobin, providing a source of hemin. Used in the cultivation of microorganisms.
Blood medium ingredient comprised of horse (Equus caballus) blood, where potassium oxalate has been added to prevent coagulation. Used in the cultivation of microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, Oxalated is horse blood that has been treated with potassium oxalate as an anticoagulant.
Blood medium ingredient comprised of horse (Equus caballus) blood where heparin has been added to prevent coagulation. Used in the cultivation of microorganisms.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Horse Blood, with Heparin is whole horse blood treated with the anticoagulant heparin. Heparin-binding of Mycobacterium tuberculosis has been suggested to increase the organism's virulence in primary infection and in extrapulmonary dissemination(5).
An organic-rich, solid microbiological culture medium comprised of peptone, meat extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1.pdf 1. NUTRIENT AGAR Peptone 5.0 g Meat extract 3.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 29601 Achromobacter animicus
An organic-rich, solid microbiological culture medium containing Bacto peptone, yeast extract and glucose. Used for the cultivation of Zymomonas.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium10.pdf 10. ZYMOMONAS MEDIUM Bacto peptone 10.0 g Yeast extract 10.0 g Glucose 20.0 g Agar if required 15.0 g Distilled water 1000.0 ml © 2012 DSMZ GmbH - All rights reserved
An organic-rich, solid microbiological culture medium containing beef extract, peptone and sodium chloride (30g/L). Used for the cultivation of general heterotrophic microorganisms.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium101.pdf 101. NUTRIENT AGAR or BROTH WITH NaCl Peptone 5.0 g Meat extract 3.0 g NaCl 30.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml © 2010 DSMZ GmbH - All rights reserved
An organic-rich, liquid microbiological culture medium containing beef extract, peptone and sodium chloride (30g/L). Used for the cultivation of general heterotrophic microorganisms.
An organic-rich, solid microbiological culture medium containing sodium caseinate, yeast extract, proteose peptone, potassium phosphate, and agar. Used for the cultivation of Flavobacterium aquatile.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium102.pdf 102. FLAVOBACTERIUM AQUATILE MEDIUM Na-caseinate 2.0 g Yeast extract 0.5 g Proteose peptone 1.0 g K2HPO4 0.5 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, ferric sulfate, nickel chloride, sotium selenate, sodium silicate, and trace minerals. Prepared under at atmosphere of hydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1000.pdf 1000. MJANHOX-NO3 MEDIUM WITH SUPPLEMENT NaCl 3.000 g K2HPO4 0.014 g CaCl2 x 2 H2O 0.014 g NH4Cl 0.040 g MgSO4 x 7 H2O 0.340 g MgCl2 x 6 H20 0.418 g KCl 0.033 g Fe2(SO4)3 x X H2O 0.005 g NiCl2 x 6 H2O 0.005 mg Na2SeO3 x 5 H2O 0.005 mg Na2SiO3 x 9 H20 0.500 g Trace mineral solution (see below) 1.000 ml Distilled water 1000.000 ml Before autoclaving, the pH of the Medium is adjusted with NaOH to 7.5-8.0. After autoclaving, the filter-sterilized vitamin solution (0.1% volume), and each of the separately autoclaved, concentrated solutions are added to the medium at a final concentration described below. Then a mix gas (80% H2, 20% CO2) is purged for 5 min. Finally, the mix gas is compressed into gas phase (> 80% volume of the tube or bottle) at 3 atm. Na2S2O3 x 5 H2O 10 mM NaHCO3 0.20 % NaNO3 0.05 % Trace mineral solution: nitrilotriacetic acid 1.500 g MnSO4 x 2 H2O 0.500 g CoSO4 x 7 H2O 0.500 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g © 2007 DSMZ GmbH - All rights reserved
trace mineral solution for DSMZ Medium 1000
A trace elements solution containing nitrilotriacetic acid, manganese sulfate, cobalt sulfate, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, and sodium molybdate.
Undefined mixtures of complex organic compounds derived from the milk proteins (casein) of a mammal, where the casein has been complexed with sodium. Used in the cultivation of microorganisms.
A minerals-salts, liquid culture medium comprised of sodium bicarbonate, ammonium chloride, sodium phosphate, potassium chloride, vitamin mix, and mineral mix. Prepared under an atmosphere of dinitrogen and carbon dioxide. Sodium acetate and iron-NTA are then added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1001.pdf 1001. BASAL MEDIUM NaHCO3 2.500 g NH4Cl 0.250 g NaH2PO4 x H2O 0.600 g KCl 0.100 g Vitamin Mix (see below) 10.000 ml Mineral Mix (see below) 10.000 ml Distilled water 980.000 ml This medium should not be exposed to direct sunlight! Add all basal medium ingredients. Bring the final volume of the medium to 1.0 liter. Dispense to appropriate culture containers. Bubble the medium with 80:20 N2:CO2 (final pH sould be 6.8 to 7.0) - approximately 10 ml of media (anaerobic culture tube) should be gassed for 5 min in the aqueous phase (bubbled) and the headspace gassed for one minute prior to sealing the container. Sterilize per usual procedure. Add electron donor (Acetate-final conc. of 10 mM-recipe below) and electron acceptor (Fe(III)NTA-final conc. of 10 mM-recipe below) from sterile, anaerobic stock solutions using a sterile syringe and needle flushed with anaerobic gas. Store out of direct light. 1.0 M Acetate Stock Solution: Add 13.6 g sodium acetate to ca. 80 ml distilled water. Bring to final volume of 100 ml. Bubble with anaerobic gas (nitrogen) for 45 min. Seal and sterilize. 500 mM Fe(III)NTA Stock solution: Add 8.2 g NaHCO3 to ca. 70 ml distilled water. Add 12.8 g of Na3Nitrilotriacetic acid (NTA) to ca. 70 ml distilled water. Add 13.5 g FeCl3 x 6 H2O to ca 70 ml distilled water. Adjust pH to 6.5 using 10N NaOH. Bring solution to final volume of 100 ml. Ingredients will go into solution after stirring for ca. 15 min. Bubble with anaerobic gas (N2) for 45 min and then filter sterilize (0.2 μm filter) into a sterile, anaerobic, serum bottle. Vitamin mixture: Biotin 2.000 mg Folic acid 2.000 mg Pyridoxine HCl 10.000 mg Riboflavin 5.000 mg Thiamine 5.000 mg Nicotinic acid 5.000 mg Pantothenic acid 5.000 mg Vitamin B12 0.100 mg p-aminobenzoic acid 5.000 mg Thioctic acid 5.000 mg Distilled water 1000.000 ml Mineral mixture: NTA 1.500 g MgSO4 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.100 g CoCl2 x 6 H2O 0.100 g ZnCl2 0.130 g CuSO4 x 5 H2O 0.010 g AlK(SO4)2 x 12 H2O 0.010 g H3BO3 0.010 g Na2MoO4 0.025 g NiCl2 x 6 H2O 0.024 g Na2WO4 x 2 H2O 0.025 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved
A trace elements solution containing nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, calcium chloride, cobalt chloride, zinc chloride, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium tungstate.
A microbiological vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, riboflavin, thiamine, nicotinic acid, pantothenic acid, vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), and thioctic acid (lipoic acid).
From: http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium220.pdf 220. CASO AGAR (Merck 105458) Peptone from casein 15.0 g Peptone from soymeal 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. Medium is identical with Tryptone Soya Agar (Oxoid Cm131). For strain DSM 21449 und DSM 21450 NaCl 10.0 g/L MnSO4 10.0 mg/L For strain DSM 5746 Methanol © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1002.pdf 1002. ECTOTHIORHODOSYNUS MEDIUM KH2PO4 0.5 g NH4Cl 0.5 g NaCl 20.0 g MgCl2 x 6 H2O 0.2 g CaCl2 0.1 g Na2CO3 5.0 g NaHCO3 5.0 g Sodium acetate 1.0 g Yeast extract 0.1 g Na2S2O3 x 5H2O 0.5 g Na2S x 9 H2O 0.1 g Trace elements SL6 (see medium 27) 1.0 ml Vitamin B12 20.0 μg Distilled water 1000.0 ml Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid microbiological culture medium comprised of sodium acetate, yeast extract, sodium thiosulfate, and sodium sulfide. Used for the cultivation of Ectothiorhodosynus.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf 27. RHODOSPIRILLACEAE MEDIUM (modified) Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteiniumchloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121˚C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, mildly reducing liquid microbiological culture medium used for the cultivation of Rhodospirillaceae. Contains yeast extract, sodium succinate, ammonium acetate, iron citrate and cysteinium chloride as a reducing agent. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium27.pdf 27. RHODOSPIRILLACEAE MEDIUM (modified) Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteinium chloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121˚C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1003.pdf 1003. HYDROGEN-OXYDIZING MEDIUM Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds: MgSO4 x 7 H2O 7.00 g NaS2O3 2.00 g CaCl2 x 2 H2O 0.40 g KCl 0.48 g MgCl2 0.78 g MES 1.95 g Add solutions A, B, and D (see recipes below): Solution A 2.00 ml Solution B 1.50 ml Solution D (Trace minerals) 10.00 ml pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121˚C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP). Solution A (100x solution): NH4Cl 100.00 g MgCl2 x H2O 100.00 g CaCl2 x 2 H2O 40.00 g Distilled water 1000.00 ml Adjust the pH to 4 with HCl. Solution B (500x solution): K2HPO4 x 3 H2O 200.00 g Distilled water 1000.00 ml Trace mineral solution (100x): see next page Trace mineral solution (100x): Na-EDTA x 2 H2O 500.00 mg CoCl2 x 6 H2O 150.00 mg MnCl2 x 4 H2O 100.00 mg FeSO4 x 7 H2O 100.00 mg ZnCl2 100.00 mg AlCl3 x 6 H2O 40.00 mg Na-tungstate x 2 H2O 30.00 mg CuCl 20.00 mg Ni2SO4 x 6 H2O 20.00 mg Se-acid 10.00 mg HBO3 10.00 mg Na2MoO4 x 2 H2O 10.00 mg Distilled water 1000.00 ml Adjust the pH of the solution to 3.0 with HCl. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containing magnesium sulfate, sodium thiosulfate, MES, hydrogen and oxygen. Used to support the growth of slighly acidophilic hydrogen-oxidizing bacteria.
trace mineral solution for DSMZ Medium 1003
A trace elements solution containing sodium EDTA, cobalt chloride, manganese chloride, ferrous sulfate zinc chloride, aluminum trichloride, sodium tungstate, copper chloride, nickel sulfate, selenic acid (se-acid), boric acid, and sodium molybdate.
A minerals-salts liquid medium containing potassium phosphate, magnesium chloride, calcium chloride, ammonium chloride, trace elements, selenite-tungstate solution, yeast extract, resazurin, sodium bicarbonate, vitamin solution, D-glucose, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. Used to support the growth of Anaerolinea.
DSM strains: 16554 Anaerolinea thermolimosa 14523 Anaerolinea thermophila UNI-1 17877 Bellilinea caldifistulae 14535 Caldilinea aerophila DSM 14535 = NBRC 104270 22659 Caldilinea tarbellica 16556 Leptolinea tardivitalis 16555 Levilinea saccharolytica 23815 Ornatilinea apprima
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1004.pdf 1004. ANAEROLINEA MEDIUM KH2PO4 0.14 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.54 g Trace element solution SL-11 (see medium 784) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.30 g Resazurin 1.00 mg NaHCO3 2.50 g Vitamins solution (see medium 141) 10.00 ml D-Glucose 2.20 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, glucose and reducing agents), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add solid bicarbonate and adjust pH to 7.0. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration), sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. The final pH should be 7.0. For DSM 16554, DSM 16555 and DSM 17877 replace glucose with 7.20 g/l sucrose and reduce amount of yeast extract to 0.10 g/l. For DSM 16556 omit glucose and reduce amount of yeast extract to 0.10 g/l. For DSM 22659 increase amount of glucose to 5.00 g/l. For DSM 23815 replace glucose with 2.00 g/l cellobiose added after autoclaving from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 1004.1 -< for DSM 16554, DSM 16555, and DSM 17877
A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted, sucrose is added, and the amount of yeast extract is reduced.
DSM 16554 is Anaerolinea thermolimosa DSM 16555 is Levilinea saccharolytica DSM 17877 is Bellilinea caldifistulae
A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted and the amount of yeast extract is reduced.
A minerals-salts liquid medium similar to DSMZ Medium 1004, except that the amount of glucose is increased.
A minerals-salts liquid medium similar to DSMZ Medium 1004, except that glucose is omitted and cellobiose added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium784.pdf 784. NATRONIELLA MEDIUM KH2PO4 0.2 g MgCl2 x 6 H2O 0.1 g NH4Cl 1.0 g KCl 0.2 g NaCl 16.0 g Trace element solution SL-11 (see below) 1.0 ml Yeast extract 0.2 g Resazurin 0.5 mg Na2CO3 68.0 g NaHCO3 38.0 g Ethanol 5.0 ml Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 1.0 g Distilled water 1000.0 ml Trace element solution SL-11: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2Mo4 x 2 H2O 36.0 mg Distilled water 1000.0 ml Adjust pH of solution to 6.0. Dissolve ingredients except carbonates, ethanol, vitamins and sulfide. Bring medium to the boil, then cool to room temperature while flushing with 100% N2 gas. Add and dissolve carbonates and sulfide while gassing the head space only. Dispense and autoclave under N2 gas atmosphere. Before use add ethanol and vitamins from sterile anoxic stock solution prepared under N2. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 9.5 – 10.0. For DSM 24923 omit ethanol and add to the autoclaved medium 2.0 g/l Na2S2O3 x 5 H2O from a sterile anoxic stock solution. © 2013 DSMZ GmbH - All rights reserved
DSM strains: 24912 Natranaerobaculum magadiense 9952 Natroniella acetigena
A minerals-salts liquid medium containing potassium phosphate, magnesium chloride, ammonium chloride, potassium chloride, sodium chloride, trace elements, yeast extract, resazurin, sodium carbonate and sodium bicarbonate, ethanol, vitamin solution, and sodium sulfide. Prepared under an atmosphere of nitrogen. Used to support the growth of Natroniella.
A minerals-salts liquid medium similar to DSMZ Medium 784, except that ethanol is omitted and sodium thiosulfate is added.
DSM 24923 = Natranaerobaculum magadiense Z1001
A trace elements solution containing disodium EDTA, ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate.
From: https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/AnimalBloodProd.htm Washed, Sheep Cell Suspensions are stabilized preparations of concentrated erythrocytes. A working solution is prepared by diluting the concentrated suspension. It is not recommended that the suspension be washed.
Blood medium ingredient comprised of red blood cells from a sheep (Ovis aries) that have been washed. Used to support the growth of microorganisms which need washed red blood cells to grow.
A minerals-salts, liquid medium containing sodium sulfate, potassium phosphate, ammonium chloride, magnesium chloride, potassium chloride, calcium chloride, resazurin, trace element solution, sodium bicarbonate, selenite-tungstate solution, sodium benzoate, pyrocatechol (i.e. catechol), hydrochloric acid, vitamin solution, and sodium sulfide. Prepared under an atmosphere of nitrogen and carbon dioxide. Used to support the growth of Desulfobacterium catecholicum.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium385.pdf 385. DESULFOBACTERIUM CATECHOLICUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 100.00 ml Solution D: Selenite-tungstate solution (see below) 1.00 ml Solution E: Na-benzoate 0.40 g Distilled water 10.00 ml Solution F: Pyrocatechol 0.06 g 1 N HCl 0.06 ml Distilled water 10.00 ml Solution G: Vitamin solution (see medium 141) 10.00 ml Solution H: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and H are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions E, F and G are prepared under N2 gas and sterilized by filtration. The pyrocatechol stock solution has to be prepared always freshly prior to use. Solution B to H are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 6.9 - 7.1. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 10 - 20% inoculum. Selenite-tungstate solution: NaOH 0.5 g Na2SeO3 x 5 H2O 3.0 mg Na2WO4 x 2 H2O 4.0 mg Distilled water 1000.0 ml © 2014 DSMZ GmbH - All rights reserved
A trace element solution containing sodium hydroxide, sodium selenite, and sodium tungstate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141.pdf 141. METHANOGENIUM MEDIUM (H2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see below) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see below) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.18 g CuSO4 x 5 H2O 0.01 g KAl(SO4)2 x 12 H2O 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.03 g Na2SeO3 x 5 H2O 0.30 mg Na2WO4 x 2 H2O 0.40 mg Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml For DSM 1498, DSM 15558, and DSM 22353 adjust pH to 6.5. For DSM 2373 increase the amount of trypticase to 6.00 g/l. For DSM 4254 add a filter-sterilized, anoxic solution of L-histidine to a final concentration of 0.08 g/l. For DSM 7268, DSM 7466, and DSM 15558 use only one atmosphere overpressure of 80% H2 and 20% CO2. For DSM 15219, DSM 16458, DSM 18860, and DSM 21220 adjust pH to 7.5. For DSM 15558 and DSM 16458 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. Use only one atmosphere overpressure of 80% H2 and 20% CO2. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containind potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypicase peptone, resazurin, sodium bicarbonate, vitamin solution, L-cysteine, and sodium sulfide. Prepared under an atmosphere of hydrogen, carbon dioxide, and nitrogen. Used to support the growth of Methanogenium.
trace elements solution for DSMZ Medium 141
A trace elements solution contining nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, sodium selenite, and sodium tungstate.
A minerals-salts liquid medium similar to DSMZ Medium 141, except that the pH has been lowered to 6.5.
DSMZ Medium 141.1 -< for DSM 1498, DSM 15558, and DSM 22353
DSM 1498 is Methanoculleus marisnigri DSM 15558 is Methanogenium marinum DSM 22353 is Methanobacterium petrolearium
DSM 2373 is Methanoculleus thermophilus DSM 2373
A minerals-salts liquid medium similar to DSMZ Medium 141, except that the concentration of trypicase peptone is increased.
A minerals-salts liquid medium similar to DSMZ Medium 141, except that L-histidine has been added.
DSM 7268 is Methanothermobacter thermoflexus DSM 7466 is Methanothermobacter defluvii DSM 15558 is Methanogenium marinum
DSMZ Medium 141.4 -< for DSM 7268, DSM 7466, and DSM 15558
A minerals-salts liquid medium similar to DSMZ Medium 141, except that a decreased pressure of H2 and CO2 is used.
A minerals-salts liquid medium similar to DSMZ Medium 141, except that the pH has been increased to 7.5.
DSM 15219 is Methanobacterium aarhusense DSM 16458 is Methanogenium frigidum DSM 18860 is Methanoculleus receptaculi DSM 21220 is not in www.dsmz. de, TaxBrowser, or StrainInfo.
A minerals-salts liquid medium similar to DSMZ Medium 141, except that coenzyme M is added and a decreased pressure of H2 and CO2 is used.
DSM 15558 is Methanogenium marinum DSM 16458 is Methanogenium frigidum Ace-2
DSMZ Medium 141.6 -< for DSM 15558 and DSM 16458
A vitamin solution containing biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, calcium pantothenate, vitamin B12 (cobalamin), and lipoic acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1005.pdf 1005. THERMODESULFOBIUM MEDIUM K2HPO4 0.78 g KH2PO4 0.75 g Na3EDTA 0.04 g FeSO4 x 7 H2O 0.01 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.03 g NaCl 0.20 g NH4Cl 0.50 g Na2SO4 2.80 g Na-acetate 0.15 g Vitamin solution (see medium 141) 10.00 ml Trace element solution SL-9 (see medium 318) 10.00 ml L-Cysteine-HCl x H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere and adjust pH to 5.5 with 10N H2SO4. Dispense under same gas atmosphere in suitable culture vessels (e.g. 20 ml of the medium in 50 ml serum bottles) and autoclave. Prior to inoculation add cysteine from a sterile, anoxic stock solution prepared under N2. Adjust pH to 5.5-6.0, if necessary. After inoculation pressurize vials to 0.5 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containing potassium phosphate, trisodium EDTA, ferrous sulfate, magnesium sulfate, calcium chloride, sodium chloride, ammonium chloride, sodium sulfate, sodium acetate, vitamin solution, trace elements, and L-cysteine hydrochloride. Prepared under an atmosphere of hydrogen, carbon dioxide, and nitrogen. Used to support the growth of Thermodesulfobium.
A minerals-salts, liquid medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamin solution, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of nitrogen and carbon dioxide. Used to support the growth of Methanosarcina.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318.pdf 318. METHANOSARCINA (BCYT) MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see below) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BBL) 0.50 g Resazurin 0.50 mg KHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 5.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic tubes under the same gas atmosphere and autoclave. Add vitamins (sterilized by filtration), methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8. Trace elements: Nitrilotriacetic acid (NTA) 12.800 g FeCl3 x 6 H2O 1.350 g MnCl2 x 4 H2O 0.100 g CoCl2 x 6 H2O 0.024 g CaCl2 x 2 H2O 0.100 g ZnCl2 0.100 g CuCl2 x 2 H2O 0.025 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.024 g NaCl 1.000 g NiCl2 x 6 H2O 0.120 g Na2SeO3 x 5 H2O 0.026 g Distilled water 1000.000 ml First dissolve NTA in 200 ml of distilled water and adjust pH to 6.5 with KOH, then dissolve mineral salts. © 2014 DSMZ GmbH - All rights reserved
A trace elements solution containing nitrilotriacetic acid, ferric trichloride, manganese chloride, cobalt chloride, calcium chloride, zinc chloride, copper chloride, boric acid, sodium molybdate, sodium chloride, nickel chloride, and sodium selenite.
An organic rich, solid medium containing yeast extract, soluble starch, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1027.pdf 1027. YEAST STARCH AGAR (A) yeast extract 2.0 g soluble starch 10.0 g agar 15.0 g distilled water 1.0 l adjust pH to 7.3 © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid medium containing xylan, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1026.pdf 1026. XED-AGAR Xylan 7.0 g Yeast extract 3.0 g Agar 18.0 g Distilled water 1000.0 ml pH 7.0 © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium containing sea salts, ammonium chloride, peptone, yeast extract, riboflavin, and glucose. Used for the cultivation of Maricaulis.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1025.pdf 1025. MARICAULIS MEDIUM Sigma Sea salts 30.0 g NH4Cl 0.5 g Distilled water 1000.0 ml Autoclave and cool the medium. Add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium. 50xPYE: Peptone 100.0 g Yeast extract 50.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An undefined inorganic chemical mixture comprised of a mixture of salts derived from the evaporation of seawater.
Inorganic compounds or mixtures of inorganic compounds added to culture media to support growth or metabolism of a microorganism. Because the exact composition of the mixture is unknown, it is referred to as "undefined".
A minerals-salts, liquid medium comprised of sodium nitrate, sodium bicarbonate, sodium thiosulfate, elemental sulfur, vitamin solution, and synthetic seawater. Prepared under an atmosphere of dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1024.pdf 1024. NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2S2O3 x 5 H20 1.000 g Sulfur,powdered 3.000 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see medium 997) 1000.000 ml Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution. Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0. © 2009 DSMZ GmbH - All rights reserved
Defined organic chemical mixture which is a solution of organic chemicals in water.
trace mineral solution for DSMZ Medium 997
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium997.pdf 997. HNW MEDIUM NaNO3 1.000 g NaHCO3 1.000 g Na2WO4 x 2 H2O 0.100 mg Na2S 0.500 g Trace vitamin solution (see medium 141) 10.000 ml DMJ synthetic seawater (see below) 1000.000 ml To prepare the medium, all compounds of DMJ seawater were dissolved in 1 liter of distilled deionized water, and the pH was adjusted to around 7.0 with NaOH at room temperature prior to autoclaving. After autoclaving, filter-sterilized NaNO3 solution (100 g/l), NaHCO3 solution (100 g/l), Na2S solution (100 g/l); pH 7.5) and trace vitamin solution were added. Then the tubes were tightly sealed with butyl rubber stoppers under a gas phase of 80% H2 + 20% CO2 (300 kPa). Synthetic seawater: NaCl 30.000 g K2HPO4 0.140 g CaCl2 x 2 H2O 0.140 g NH4Cl 0.250 g MgSO4 x 7 H2O 3.400 g MgCl2 x 6 H2O 4.180 g KCl 0.330 g NiCl2 x 6 H2O 0.500 mg Na2SeO3 x 5 H2O 0.500 mg Fe(NH4)2(SO4)2 x 6 H2O 0.010 g Trace mineral solution (see below) 10.000 ml Trace mineral solution: C6H9NO6 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x 2 H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg © 2007 DSMZ GmbH - All rights reserved
A trace elements solution containing C6H9NO6 (nitrilotriacetic acid), magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium selenite.
DMJ synthetic seawater solution for DSMZ Medium 997
An inorganic salts solution comprising a synthetic seawater. Comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium selenite, ferrous ammonium sulfate, and trace minerals.
DSM strains: 15103 Persephonella hydrogeniphila 16510 Hydrogenivirga caldilitoris
A minerals-salts, liuid medium containing sodium nitrate, sodium bicarbonate, sodium tungstate, sodium sulfide, vitamin solution, and synthetic seawater. Prepared under an atmosphere of dihydrogen and carbon dioxide.
A solution of inorganic (mostly non-metal) salts. Used to support the growth of microorganisms.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1023.pdf 1023. MANNING MEDIUM (NH4)2SO4 6.00 g KCl 0.20 g K2HPO4 0.20 g MgSO4 x 7 H2O 1.00 g Ca(NO3)2 0.02 g Distilled water 1000.00 ml Solution A: FeSO4 x 7 H2O 33.40 g/l Solution B: Yeast extract 0.20 g/l Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts liquid medium that contains ammonium sulfate, potassium chloride, potassium phosphate, calcium nitrate, ferrous sulfate, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1021.pdf 1021. SODALIS GLOSSINIDIUS MEDIUM Solution A: Yeast extract 6.20 g Lactalbumin hydrolysate or Casein hydrolysate 8.10 g D-Glucose 5.00 g KCl 0.25 g MgCl2 x 6 H2O 0.12 g CaCl2 x 2 H2O 0.25 g NaCl 8.70 g NaHCO3 0.15 g Agar 17.00 g Distilled water 900.00 ml Adjust pH to 8.0. Solution B: NaH2PO4 x 2 H2O 0.28 g Distilled water 100.00 ml Sterilize solution A and B separately at 121˚C for 20 min. Cool to 50˚C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid medium containing yeast extract, lactalbumin hydrolysate or casein hydrolysate, D-glucose, potassium chloride, magnesium chloride, calcium chloride, sodium chloride, sodium bicarbonate, agar, sodium phosphate, and fetal calf serum or calf serum. Used for the cultivation of Sodalis glossinidius.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium503.pdf 503. FWM MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Resazurin 0.50 mg Distilled water 940.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: Vitamins solution (see below) 1.00 ml Solution D: Selenite-tungstate solution (see medium 385) 1.00 ml Solution E: NaHCO3, 5% w/v solution 50.00 ml Solution F: Substrate solution (see below) 10.00 ml Solution G: Na2S x 9 H2O, 3% w/v solution 10.00 ml Prepare solution A anoxically under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions B, C, D, F and G are prepared separately under 100% N2 gas. Solution E is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize the vitamins solution. Solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust final pH of medium to 7.2 - 7.4, if necessary. Seven vitamins solution: Vitamin B12 100.00 mg p-Aminobenzoic acid 80.00 mg D(+)-Biotin 20.00 mg Nicotinic acid 200.00 mg Calcium pantothenate 100.00 mg Pyridoxine hydrochloride 300.00 mg Thiamine-HCl x 2 H2O 200.00 mg Distilled water 1000.00 ml For DSM 5847: Use 1.50 g/l of Na-(D/L)-3-hydroxybutyrate or 2.00 g/l D-fructose as substrate. For DSM 5849: Use 2.50 g/l of Na2-succinate as substrate. For DSM 5885: Use 1.60 g/l Na2-maleate as substrate. Adjust pH of completed medium to 6.7 - 6.8. For DSM 6779: Use 2.00 g/l of xylan or xylose as substrate. For DSM 10092: Add 0.70 g/l Na2SO4 to solution A and use 1.50 g/l Na-propionate as substrate. Reduce the amount of sulfide to 0.10 g/l and use 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) for reduction of the medium prior to inoculation. For DSM 11046: Add 1.70 g/l NaNO3 to solution A and use 1.25 g/l taurine as substrate. For DSM 11261: Add 1.00 g/l yeast extract to solution A and use 2.00 g/l Na-glycolate as substrate. For DSM 11262: Add 1.00 g/l yeast extract to solution A and use 2.50 g/l Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 11263 and DSM 11489: Use 0.82 g/l Na-acetate and 3.20 g/l Na2-fumarate as substrates. For DSM 11270: Use 2.50 g/l taurine as substrate. For DSM 11480: Use 1.80 g/l D-glucose as substrate added from an anoxic stock solution sterilized by filtration. For DSM 12018: Use 0.20 g/l yeast extract and 2.66 g/l L-aspartic acid as substrates. For DSM 13305: Add 1.00 g/l yeast extract to solution A. Use 5.00 g/l D-fructose as substrate. For DSM 14424 and DSM 28450: Use 5.00 g/l glucose and 2.00 g/l yeast extract as substrates. For DSM 15206: Use 2.00 g/l yeast extract and 2.00 g/l Trypticase peptone as substrates. For DSM 15978 and DSM 27305: Use 2.00 g/l trimethylamine hydrochloride and 0.60 g/l methanol as substrates and adjust pH of the medium to 6.5 - 7.0. After inoculation add 10 ml/l of a sterile, anoxic stock solution of FeSO4 x 7 H2O (0.2% w/v). For DSM 16082: Add 1.40 g/l Na2SO4 to solution A. Distribute aliquots of 50 ml solution A in 120 ml serum bottles, autoclave and complete medium with solutions B to G. Use as substrate 1.00 ml of a 2,2,4,4,6,8,8-heptamethylnonane (Aldrich) solution containing 1.5% m-xylene. Prior to inoculation add to the completed medium 0.53 ml of a sterile, anoxic stock solution of FeSO4 x 7 H2O (8% w/v) in 0.25 N H2SO4. Adjust pH of the completed medium to 7.2, if necessary. For DSM 21662: Replace solution B and D with the trace elements solution of medium 141, solution C with the vitamins solution of medium 141 and omit solution G. Use 1.36 g/l Na-acetate as substrate. Prior to inoculation 1.22 g/l Na-perchlorate is added from a sterile anoxic stock solution sterilized by filtration. For DSM 24856: Add 0.85 g/l NaNO3 to solution A. Use 0.10 g/l yeast extract and 1.10 g/l Na-pyruvate as substrates. Na-pyruvate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. For DSM 24984: Use 0.80 g/l Na-acetate and 3.10 g/l Na2HAsO4 x 7 H2O as substrates. Na2-arsenate is added to the autoclaved medium from a sterile anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containing potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, resazurin, trace elements, vitamin solution, selenite-tungstate solution, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSM strains: 25964 Aminivibrio pyruvatiphilus 19636 Ammonifex thiophilus 11045 Bilophila wadsworthia 11046 Castellaniella denitrificans 5847 Clostridium homopropionicum DSM 5847 11261 Lachnospiraceae bacterium 19gly4 15206 Clostridium tunisiense TJ 6779 Cytophaga xylanolytica 5651 Desulfococcus biacutus 16082 Desulfotomaculum sp. Ox39 11493 Desulfovibrio 11489 Geobacter 11263 Geovibrio thiophilus 21662 Magnetospirillum bellicus 15978 Methanomethylovorans hollandica DSM 15978 27305 Methanomethylovorans uponensis 14424 Opitutus sp. VeGlc2 15970 Parasporobacterium paucivorans DSM 15970 12018 Propionivibrio pelophilus DSM 12018 5849 Propionivibrio 24984 Seleniivibrio woodruffii 24856 Thermoanaerobaculum aquaticum 11262 Peptostreptococcaceae bacterium 19gly3
seven vitamins solution for DSMZ medium 503
A vitamin solution comprised of vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), biotin, nicotinic acid, calcium pantothenate, pyridoxine hydrochloride, and thiamine hydrochloride.
Similar to DSMZ Medium 503, except adds 3-hydroxybutyrate or D-fructose as a substrate.
DSM 5847 is Clostridium homopropionicum DSM 5847
A serum medium ingredient, derived from the serum (coagulated blood) from a fetal calf of a slaughtered cow (Bos taurus).
Wikipedia: Fetal bovine serum Fetal bovine serum (FBS) or fetal calf serum is the blood fraction remaining after the natural coagulation of blood, followed by centrifugation to remove any remaining red blood cells.[1] Fetal bovine serum comes from the blood drawn from a bovine fetus via a closed system of collection at the slaughterhouse. Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. The globular protein, bovine serum albumin (BSA), is a major component of fetal bovine serum. The rich variety of proteins in fetal bovine serum maintains cultured cells in a medium in which they can survive, grow, and divide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1016.pdf 1016. IDIOMARINA MEDIUM Glucose 10.0 g Proteose peptone 5.0 g Yeast extract 3.0 g Malt extract 3.0 g NaCl 30.0-60.0 g Agar (if required) 18.0 g Distilled water 1000.0 ml DSM 16139 and DSM 16140 have been grown in medium containing 45 g/l NaCl. © 2007 DSMZ GmbH - All rights reserved
DSM 16139 is Idiomarina fontislapidosi DSM 16140 is Idiomarina ramblicola
An organic-rich, solid medium containing glucose, proteose peptone, yeast extract, malt extract, sodium chloride (45 grams per liter), and agar.
DSMZ Medium 1016.1 -< for DSM 16139 and DSM 16140
An organic-rich, solid medium containing glucose, proteose peptone, yeast extract, malt extract, sodium chloride (variable amounts, from 30 to 60 grams per liter), and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1017.pdf 1017. YPG MEDIUM Yeast extract 10.0 g/l Peptone (Difco) 10.0 g/l Glucose 70.0 g/l Distilled water 1000.0 ml Adjust the pH to 6.0 with dilute HCl. Solid media may be prepared by adding 15g/l agar. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid medium containing yeast extract, peptone, and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1018.pdf 1018. HALOFERAX SULFURIFONTIS MEDIUM Yeast extract 5.0 g/l NaCl 150.0 g/l MgCl2 20.0 g/l K2SO4 0.5 g/l CaCl2 0.1 g/l Distilled water 1000.0 ml pH 7.0 The medium may be solidified by adding 20 g/l agar. © 2007 DSMZ GmbH - All rights reserved
An organic rich, liquid medium containing yeast extract, sodium chloride, magnesium chloride, potassium sulfate, and calcium chloride.
trace elements solution for DSMZ Medium 1019
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1019.pdf 1019. ROUF'S MEDIUM Yeast extract 5.00 g Peptone 5.00 g MgSO4 x 7 H2O 0.20 g CaCl2 x 6 H2O 0.05 g Fe(NH3)citrate 0.015 g MnSO4 x 4 H2O 0.05 g FeCl3 x 4 H2O 0.01 g Vitamin solution (see medium 141) 10.00 ml Trace elements solution (see below) 1.00 ml Distilled water 1000.00 ml pH 7.1 (adjusted with NaOH if needed) The medium may be solifidied with 17 g/l agar. Trace elements solution: Nitrilotriacetic acid 12.80 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g FeSO4 x 7 H20 0.10 g MnCl2 x 4 H2O 0.10 g NaCl 0.10 g Na2MoO4 x 2 H2O 0.10 g Distilled water 1000.00 ml First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. © 2007 DSMZ GmbH - All rights reserved
A trace elements solution containing nitrilotriacetic acid, cobalt chloride, calcium chloride, ferrous sulfate, manganese chloride, sodium chloride, and sodium molybdate.
An organic-rich, liquid medium containing yeast extract, peptone, magnesium sulfate, calcium chloride, ferric ammonium citrate, manganese sulfate, iron trichloride, vitamin solution, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1020.pdf 1020. MBM MEDIUM (modified) NaNO3 0.2 g KH2PO4 0.2 g NH4Cl 0.2 g MgCl2 x 6 H2O 0.4 g KCl 0.2 g CaCl2 x 2 H2O 0.1 g Na2S2O3 x 5 H2O 2.5 g SL-4 trace element solution (see medium 14) 2.0 ml Resazurin 1.0 mg Distilled water 1000.0 ml Dissolve ingredients, except SL-4 trace element solution and sodium thiosulfate, then flush solution with 80% N2 and 20% CO2 gas mixture to make it anoxic. Distribute medium under same gas atmosphere into culture vessels (e.g. 20 ml in 120 ml serum bottles) and autoclave. Filter-sterilize under anoxic conditions the SL-4 trace element solution and a stock solution of sodium thiosulfate. Prior to inoculation, add the trace element solution and sodium thiosulfate to the medium, and adjust pH to 7.0 if necessary. After inoculation, pressurize culture vessels to 0.5 bar overpressure with 100% H2 gas. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containing sodium nitrate, potassium phosphate, ammonium chloride, mangesium chloride, potassium chloride, calcium chloride, sodium thiosulfate, trace elements, and resazurin. Prepared under an atmosphere of nitrogen, carbon dioxide, and hydrogen.
http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=LP0027&c=uk&lang=EN LIVER DIGEST NEUTRALISED Code: LP0027 A biologically standardised papaic digest of liver for use as a source of nutrients in microbiological culture media. The digest is water soluble and compatible with other culture media ingredients and may be sterilised by filtration or autoclaving; thus it is suitable for use as an integral part of many culture media or as a valuable supplement. Being derived from liver this product contains relatively high levels of iron. The profile shows the characteristic even spread of peptides obtained from papaic digests.
Similar to DSMZ Medium 503, except adds sodium succinate as a substrate.
Similar to DSMZ Medium 503, except adds sodium maleate as a substrate and adjusts the pH down to 6.7-6.8.
DSM 5885 is Propionivibrio dicarboxylicus DSM 5885
Similar to DSMZ Medium 503, except that xylan or xylose is added as a substate.
Similar to DSMZ Medium 503, except that sodium sulfate is added and sodium propionate is added as a substrate. The amount of sodium sulfide is reduced and sodium dithionite is added.
Similar to DSMZ Medium 503, except sodium nitrate is added and taurine is added as a substrate.
DSM 11046 is Castellaniella denitrificans
Similar to DSMZ Medium 503, except yeast extract and sodium glycolate are added as substrates.
DSM 11261 is Lachnospiraceae bacterium 19gly4
DSM 11262 is Peptostreptococcaceae bacterium 19gly3
Similar to DSMZ Medium 503, except yeast extract and 3-hydroxybutyrate are added as substrates.
DSMZ Medium 503.9 -< for DSM 11263 and DSM 11489
Similar to DSMZ Medium 503, except sodium acetate and sodium fumarate are added as substrates.
DSM 11263 is Geovibrio thiophilus DSM 11489 is Geobacter sp.
Similar to DSMZ Medium 503, except traurine is added as a substrate.
DSM 11270 is Desulfonispora thiosulfatigenes DSM 11270
DSM 11480 is "unclassified bacterium". No sequences associated with this strain. Not in Tax Browser.
Similar to DSMZ Medium 503, except D-glucose is added as a substrate.
DSM 12018 is Propionivibrio pelophilus DSM 12018
Similar to DSMZ Medium 503, except yeast extract and L-aspartic acid are added as substrates.
Similar to DSMZ Medium 503, except yeast extract and D-fructose are added as substrates.
DSM 13305 is Propionispora vibrioides DSM 13305
Similar to DSMZ Medium 503, except glucose and yeast extract are added as substrates.
DSMZ Medium 503.14 -< for DSM 14424 and DSM 28450
DSM 14424 is Opitutus sp. VeGlc2 DSM 28450 is Fusibacter sp. KhalAKB1
Similar to DSMZ Medium 503, except yeast extract and trypticase peptone are added as substrates.
DSM 15978 is Methanomethylovorans hollandica DSM 15978 DSM 27305 is Methanomethylovorans uponensis
DSMZ Medium 503.16 -< for DSM 15978 and DSM 27305
Similar to DSMZ Medium 503, except trimethylamine hydrochloride, methanol, and ferrous sulfate heptahydrate are added as substrates, and the pH is lowered to 6.5-7.0.
Similar to DSMZ Medium 503, except sodium sulfate, 2,2,4,4,6,8,8-heptamethylnonane, m-xylene, ferrous sulfate heptahydrate, and sulfuric acid are added.
Similar to DSMZ Medium 503, except trace elements solution SL-10 and selenite-tungstate solution are replaced with the trace elements of medium 141, solution C with the vitamins solution of medium 141, and sodium sulfide is omitted. Added is sodium acetate and sodium perchlorate.
An inorganic salts solution containing potassium phosphate, sodium chloride, ammonium sulfate, calcium chloride, and magnesium sulfate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium330.pdf 330. RUMEN BACTERIA MEDIUM Mineral solution (see below) 38.00 ml K2HPO4 0.30 g Trypticase peptone (BD BBL) 2.00 g Yeast extract 0.50 g Volatile fatty acid mixture (see below) 3.10 ml Haemin 1.00 mg Glycerol 0.50 g Resazurin 1.00 mg Na2CO3 4.00 g D-Glucose 0.50 g Maltose 0.50 g Cellobiose 0.50 g Starch, soluble 0.50 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 960.00 ml Dissolve ingredients (except carbonate, carbohydrates, cysteine, and sulfide), bring medium to the boil, then cool to room temperature while gassing with 100% CO2 gas. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere in rubber stoppered tubes (i.e. Hungate-type tubes) and autoclave. Thereafter, add carbohydrates, cysteine and sulfide from sterile stock solutions (prepared anoxically under 100% N2 gas and autoclaved). Adjust pH of final medium to 6.7 - 6.8, if necessary. Mineral solution: KH2PO4 6.00 g NaCl 12.00 g (NH4)2SO4 6.00 g CaCl2 x 2 H2O 1.60 g MgSO4 x 7 H2O 2.50 g Distilled water 1000.00 ml Volatile fatty acid mixture: Acetic acid 4.25 ml Propionic acid 1.50 ml Butyric acid 1.00 ml n-Valeric acid 0.25 ml iso-Butyric acid 0.25 ml DL-2-Methyl butyric acid 0.25 ml iso-Valeric acid 0.25 ml © 2014 DSMZ GmbH - All rights reserved
An organic rich, liquid medium containing mineral solution, potassium phosphate, trypticase peptone, yeast extract, volatile fatty acids, hemin, glycerol, resazurin, sodium carbonate, D-glucose, maltose, cellobiose, soluble starch, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of carbon dioxide and nitrogen. Used for the cultivation of rumen bacteria.
volatile fatty acid mixture for DSMZ Medium 330
DSMZ Medium 330: 330. RUMEN BACTERIA MEDIUM Mineral solution (see below) 38.00 ml K2HPO4 0.30 g Trypticase peptone (BD BBL) 2.00 g Yeast extract 0.50 g Volatile fatty acid mixture (see below) 3.10 ml Haemin 1.00 mg Glycerol 0.50 g Resazurin 1.00 mg Na2CO3 4.00 g D-Glucose 0.50 g Maltose 0.50 g Cellobiose 0.50 g Starch, soluble 0.50 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 960.00 ml Dissolve ingredients (except carbonate, carbohydrates, cysteine, and sulfide), bring medium to the boil, then cool to room temperature while gassing with 100% CO2 gas. Add the carbonate and equilibrate the medium with the CO2 gas to pH 6.8. Distribute under CO2 gas atmosphere in rubber stoppered tubes (i.e. Hungate-type tubes) and autoclave. Thereafter, add carbohydrates, cysteine and sulfide from sterile stock solutions (prepared anoxically under 100% N2 gas and autoclaved). Adjust pH of final medium to 6.7 - 6.8, if necessary. Mineral solution: KH2PO4 6.00 g NaCl 12.00 g (NH4)2SO4 6.00 g CaCl2 x 2 H2O 1.60 g MgSO4 x 7 H2O 2.50 g Distilled water 1000.00 ml Volatile fatty acid mixture: Acetic acid 4.25 ml Propionic acid 1.50 ml Butyric acid 1.00 ml n-Valeric acid 0.25 ml iso-Butyric acid 0.25 ml DL-2-Methyl butyric acid 0.25 ml iso-Valeric acid 0.25 ml © 2014 DSMZ GmbH - All rights reserved
A defined organic solution containing a mixture the following volatile fatty acids: acetic acid, propionic acid, butyric acid, valeric acid, isobutyric acid, 2-methylbutyric acid, and isovaleric acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1006.pdf 1006. ALLISONELLA MEDIUM K2HPO4 292.0 mg KH2PO4 292.0 mg (NH4)2SO4 480.0 mg NaCl 480.0 mg MgSO4 x 7 H2O 100.0 mg CaCl2 x 2 H2O 64.0 mg Trypticase 1.0 g Yeast extract 4.0 g DL-Histidine 7.8 g Resazurin 1.0 mg Na2CO3 4.0 g Volatile fatty acid mixture (see medium 330) 3.1 ml L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense under same gas atmosphere in culture vessels and autoclave. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid medium containing potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, trypticase (trypticase peptone), yeast extract, histidine, resazurin, sodium carbonate, volatile fatty acid mixture, and L-cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide.
DSM 24478 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24479 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24480 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24481 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24492 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24493 is not in www.dsmz.de, TaxBrowser, or StrainInfo. DSM 24527 is not in www.dsmz.de, TaxBrowser, or StrainInfo.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1007.pdf 1007. MINERAL MEDIUM KNO3 250.0 mg KH2PO4 100.0 mg MgSO4 x 7 H2O 50.0 mg CaCl2 x 2 H2O 10.0 mg Trace elements 1.0 ml Distilled water 1000.0 ml Trace elements: EDTA 5.00 g CuCl2 x 5 H2O 0.10 g FeSO4 x 7 H2O 2.00 g ZnSO4 x 7 H2O 0.10 g NiCl2 x 6 H2O 0.02 g CoCl2 x 6 H2O 0.20 g Na2MoO4 0.03 g MnCl2 x 4 H2O 0.03 g Distilled water 1000.00 ml Final pH 5.5-6.0. DSM 15672 may be grown on either 0.5-1.0% methanol or under a gas phase of 20% methane in air. DSM 15673 supplied from the DSMZ has been grown on methanol. DSM 16984 has been grown on 20% methane in air. DSM 24478, 24479, 24480, 24481, 24492, 24493 and 24527 have been grown on 50% methane in air. Strains should be grown with shaking when grown on methane. © 2012 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1007, except that methane (in higher amounts) is added.
Similar to DSMZ Medium 1007, except that methane is added.
Similar to DSMZ Medium 1007, except that methanol is added.
DSM 15672 is Alteromonas stellipolaris LMG 21856
Similar to DSMZ Medium 1007, except that methanol, methane, and air (oxygen) is added.
A minerals-salts, liquid medium containing potassium nitrate, potassium phosphate, magnesium sulfate, calcium chloride, and trace elements.
An organic-rich, liquid medium containing trypticase peptone, yeast extract, sodium thioglycolate, resazurin, charcoal-filtered seawater and cellobiose. Prepared under at atmosphere of dinitrogen. Used for the cultivation of marine spirochaetes.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1008.pdf 1008. MARINE SPIROCHETE MEDIUM Trypticase Peptone (BD 211921) 2.0 g Yeast extract 1.0 g Na-Thioglycolate 1.0 g Resazurin 0.5 mg Charcoal-filtered, natural seawater 800.0 ml Distilled water 200.0 ml Dissolve ingredients (except thioglycolate), boil medium for 3 min., then cool to room temperature under N2 gas atmosphere. Add thioglycolate and adjust pH of medium to 7.5 with 10 N KOH. Dispense under N2 gas atmosphere in culture vessels and autoclave at 121 ˚C for 15 min. Bottled water from Biomaris GmbH can be used instead of filtered seawater. Prepare 10% cellobiose solution (10.0 g in 100 ml distilled water) under nitrogen atmosphere, filter-sterilize and add 0.2 ml to 10 ml autoclaved medium. © 2009 DSMZ GmbH - All rights reserved
An organic-rich, liquid medium comprised of tryptone, casitone, glucose, and artificial seawater.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1009.pdf 1009. TCG MEDIUM Tryptone 3.0 g Casitone 5.0 g Glucose 4.0 g Seawater (see below) 1000.0 ml Artifical seawater (ASW, eg., Instant Ocean) = 32 g seasalt disolved in 1000 ml distilled water. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1010.pdf 1010. ARTIFICAL SEAWATER MEDIUM NaCl 26.40 g MgCl2 x 6 H2O 5.70 g MgSO4 x 7 H2O 6.80 g KCl 0.66 g CaCl2 x 2 H2O 1.47 g KBr 0.09 g Selenite-tungstate solution (see medium 385) 1.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg KH2PO4 0.20 g NH4Cl 0.25 g NaHCO3 2.50 g Na-lactate 2.30 g Vitamin solution (see medium 141) 10.00 ml Seven vitamins solution (see medium 503) 1.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave. Add dihydrogenphosphate, ammonium chloride, bicarbonate, lactate, vitamins and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas or 80% N2 and 20% CO2 gas mixture (bicarbonate). The vitamin solutions should be sterilized by filtration. Adjust the final pH of the medium to 7.2 - 7.5. For DSM 15769 replace lactate as substrate with 0.45 g/l 3-phenylpropionate, which is added to the medium after autoclaving from a sterile anoxic stock solution prepared under 100% N2.
Similar to DSMZ Medium 1010, except that lactate is omitted and 3-phenylpropionate is added.
A minerals-salts, liquid medium containing sodium chloride, magnesium chloride, magnesium sulfate, potassium chloride, calcium chloride, potassium bromide, selenite-tungstate solution, trace elements, resazurin, potassium phosphate, ammonium chloride, sodium bicarbonate, sodium lactate, vitamin solution, seven vitamins solution, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 1011, except that dinitrogen and carbon dioxide are omitted
DSM 28671 is described as Thiomicrospira crunogena strain SP-41 (strain is not in Tax Browser yet). Thiomicrospira crunogena
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011.pdf 1011. MJ MEDIUM NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under N2, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with 80% N2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 20% of the headspace. For DSM 23290 supplement medium with 2.00 g/l NaNO3. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 24660 omit thiosulfate and supplement medium with 4.00 g/l yeast extract and 4.00 g/l Trypton peptone. After autoclaving the medium is reduced with 0.30 g/l Na2S x 9 H2O added from a sterile anoxic stock solution (3% w/v) prepared under 100% N2 gas and the pH adjusted to 6.5. After inoculation pressurize vessels to 1 bar overpressure with 80% N2 and 20% CO2 gas mixture. Do not add sterile air! For DSM 28671 omit pressurizing vials with 0.5 bar overpressure of 80% N2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1011, except that sodium thiosulfate and air (oxygen) are omitted. Yeast extract, tryptone, and sodium sulfide is added. Finally, the pH is made slightly more acidic (6.5).
DSM 24660 is Thermotomaculum hydrothermale
Similar to DSMZ Medium 1011, except that sodium nitrate is added and air (oxygen) is omitted.
A minerals-salts, liquid medium containing sodium chloride, potassium phosphate, calcium chloride, magnesium sulfate, magnesium chloride, potassium chloride, ammonium chloride, ferrous ammonium sulfate, trace elements, sodium bicarbonate, sodium carbonate, and vitamins. Prepared under an atmosphere of dinitrogen, carbon dioxide, and air (oxygen).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1011a.pdf 1011a. MJ MEDIUM FOR THIOBACTER SUBTERRANEUS NaCl 3.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g MgSO4 x 7 H2O 0.34 g MgCl2 x 6 H2O 0.42 g KCl 0.33 g NH4Cl 0.25 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml NaHCO3 1.50 g Na2S2O3 x 5 H2O 1.50 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, thiosulfate and vitamins), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels (up to a volume of 20%) and autoclave. Add bicarbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under 100% N2 gas, whereas the solution of bicarbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust the final pH of the medium to 6.7. After inoculation add sterile air in an amount that is equivalent to a volume of 50% of the headspace. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1011, except that reduced amounts of sodium chloride, magnesium sulfate, and magnesium chloride are added.
An organic-rich, liquid medium containing nutrient broth medium, casamino acids, yeast extract, calcium chloride, and magnesium chloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1012.pdf 1012. DN BROTH Bacto Nutrient Broth (Difco 0003) 0.8 g Casamino acids 0.5 g Yeast extract 0.1 g CaCl2 x 2 H2O 0.3 g MgCl2 x 6 H2O 0.6 g Distilled water 1000.0 ml Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1014.pdf 1014. THIOALKALIVIBRIO HALOPHILUS MEDIUM NaCl 175.00 g K2HPO4 1.50 g NH4Cl 0.50 g Trace element solution SL-4 (see medium 14) 1.00 ml MgCl2 x 6 H2O 0.20 g MgSO4 x 7 H2O 0.25 g Na2S2O3 5.00 g NaHCO3 4.00 g Distilled water 1000.00 ml Dissolve ingredients except magnesium chloride, magnesium sulfate, thiosulfate and bicarbonat. Fill solution in screw capped Erlenmeyer flasks (1/10 volume) and autoclave at 110˚C for 30 min. Add remaining compounds from sterile stock solutions and adjust pH of the medium to 8.0 - 8.5. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid medium containing sodium chloride, potassium phosphate, ammonium chloride, trace elements, magnesium chloride, magnesium sulfate, sodium thiosulfate, and sodium bicarbonate. Used for the cultivation of Thioalkalkalivibrio halophilus.
An organic rich, solid medium containing yeast extract, peptone, glucose, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1015.pdf 1015. YPGA Yeast extract 7.0 g Bactopeptone 7.0 g Glucose 7.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium14.pdf 14. CLOSTRIDIUM FORMICOACETICUM MEDIUM D-Fructose 5.00 g Yeast extract 5.00 g K2HPO4 10.00 g NaHCO3 10.00 g Trace element solution SL-4 (see below) 10.00 ml Pyridoxine-HCl 1.00 mg Resazurin 1.00 mg Na-thioglycolate 0.75 g Agar, if necessary 15.00 g Distilled water 1000.00 ml Dissolve ingredients (except frucose and bicarbonate) and sparge medium with 100% N2 gas to make it anoxic. Add fructose from a sterile anoxic stock solution prepared under N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH to 8.0. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see medium 27) 100.00 ml Distilled water 900.00 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using a 2 M solution of NaOH; then add ferrous sulfate and the trace element solution SL–6. © 2009 DSMZ GmbH - All rights reserved
A trace elements solution containing EDTA, ferrous sulfate, and trace elements solution SL-6.
An organic rich, solid medium containing D-fructose, yeast extract, potassium phosphate, sodium bicarbonate, trace elements, pyridoxine hydrochloride, resazurin, sodium thioglycollate, and agar. Prepared under an atmosphere of nitrogen and carbon dioxide. Used for the cultivation of Clostridium formicoaceticum.
https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/fbs/other-sera/bovine-serum.html Gibco® bovine (aka calf serum) serum is collected from prime cattle with an age range of 12-36 months, but typically less than 24 months. All lots are processed and manufactured in New Zealand. Each lot of bovine serum is tested for its ability to support the growth of VERO cells over three subcultures. At each passage, the cells subcultured to the original cell-inoculation density. Results are compared to parallel results obtained using control growth medium containing a previously characterized reference serum. Bovine serum is available in a heat inactivated and non-heat inactivated format. In addition, we offer a donor bovine serum and a donor bovine serum with iron. HI serum is heated for 30 minutes at 56°C while mixing to inactivate the complement Donor bovine serum with iron contains a Ferric citrate complex at 6.0 mg/dl
A serum medium ingredient, derived from the serum (coagulated blood) from a young calf, less than 36 months old, of a slaughtered cow (Bos taurus).
DSM 24856 is Thermoanaerobaculum aquaticum
Similar to DSMZ Medium 503, except sodium nitrate, yeast extract, and sodium pyruvate are added as substrates.
Similar to DSMZ Medium 503, except sodium acetate and disodium hydrogen arsenate are added as substrates.
trace elements solution for DSMZ Medium 1007
A trace elements solution containing EDTA, copper chloride, ferrous sulfate, zinc sulfate, nickel chloride, cobalt chloride, sodium molybdate, and manganese chloride.
An undefined inorganic chemical mixture comprised of seawater that has been passed through a charcoal filter.
A water filter that uses charcoal to filter a liquid.
An inorganic salts solution that mimics sea water.
A defined inorganic chemical mixture made from a commercially available synthetic sea salt (Instant Ocean® sea salt).
From: Instant Ocean Synthetic Sea Salt MSDS AQUARIUM SYSTEMS INC -- INSTANT OCEAN SYNTHETIC SEA SALT -- 6810-00N009578 ===================== Product Identification ===================== Product ID:INSTANT OCEAN SYNTHETIC SEA SALT MSDS Date:11/17/1986 FSC:6810 NIIN:00N009578 MSDS Number: BCSTP === Responsible Party === Company Name:AQUARIUM SYSTEMS INC Address:8141 TYLER BOULEVARD City:MENTOR State:OH ZIP:44060 Country:US Info Phone Num:216-255-1997 Emergency Phone Num:216-255-1997 CAGE:56932 === Contractor Identification === Company Name:AQUARIUM SYSTEMS INC Address:8141 TYLER BOULEVARD Box:City:MENTOR State:OH ZIP:44060 Phone:216-255-1997 CAGE:56932 ============= Composition/Information on Ingredients ============= Ingred Name:CALCIUM CHLORIDE CAS:10043-52-4 RTECS #:EV9800000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:POTASSIUM CHLORIDE CAS:7447-40-7 RTECS #:TS8050000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:SODIUM SULFATE CAS:7757-82-6 RTECS #:WE1650000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:NACL COMMON SALT CAS:7647-14-5 RTECS #:VZ4725000 Fraction by Wt: 70% Other REC Limits: OSHA PEL: ACGIH TLV: Ingred Name:MAGNESIUM CHLORIDE CAS:7786-30-3 RTECS #:OM2800000 Fraction by Wt: Other REC Limits: OSHA PEL: ACGIH TLV: ===================== Hazards Identification ===================== LD50 LC50 Mixture: Routes of Entry: Inhalation:YES Skin:NO Ingestion:NO Reports of Carcinogenicity:NTP:NO IARC:NO OSHA:NO Health Hazards Acute and Chronic:CHRONIC TOXICITY:MAY BE A MILD IRRITANT.ROUTE OF MOST DETRIMENTAL EXPOS:INHALATION,TARGET ORGANS:MUCUS MEMBRANES.SKIN,EYES. Explanation of Carcinogenicity:N/A Effects of Overexposure:NOT DETERMINED Medical Cond Aggravated by Exposure: ======================= First Aid Measures ======================= First Aid:EYE & SKIN CONTACT: RINSE WITH FRESH H*2O.INGESTION:DRINK WATER TO DILUTE.INHAL:CONTROL OF DUST TO COMFORT LEVELS IN WORK AREAS. ===================== Fire Fighting Measures ===================== Extinguishing Media:NON FLAMMABLE Fire Fighting Procedures: Unusual Fire/Explosion Hazard:NONE ================== Accidental Release Measures ================== Spill Release Procedures:SWEEP UP DRY & DISCARD.IF WET MOP UP & RINSE DOWN DRAIN. Neutralizing Agent: ====================== Handling and Storage ====================== Handling and Storage Precautions:STORE IN COOL,DRY AREA TO PREVENT MOISTURE PICK-UP. Other Precautions:NO SPECIAL PRECAUTIONS. ============= Exposure Controls/Personal Protection ============= Respiratory Protection:N/R Ventilation:N/R Protective Gloves:ADVISABLE.OVEREXPOS MAY CAUSE DRY SKIN Eye Protection:SAFETY GLASSES W/ SIDE SHIELDS Other Protective Equipment: Work Hygienic Practices:NORMAL GOOD HOUSEKEEPING PRACTICES Supplemental Safety and Health STATE:SOLID ================== Physical/Chemical Properties ================== HCC:N1 Boiling Pt:B.P. Text:NA Vapor Pres:NA Spec Gravity:1.2 pH:8.3-3% Solubility in Water:35GM/100ML Appearance and Odor:WHITE MIX,POWDER/CRYSTAL,ODORLESS Percent Volatiles by Volume: Corrosion Rate:NO ================= Stability and Reactivity Data ================= Stability Indicator/Materials to Avoid:YES Stability Condition to Avoid: Hazardous Decomposition Products:NONE Conditions to Avoid Polymerization: ==================== Disposal Considerations ==================== Waste Disposal Methods:DISPOSE OF IAW ALL FEDERAL,STATE & LOCAL REGULATIONS. Disclaimer (provided with this information by the compiling agencies): This information is formulated for use by elements of the Department of Defense. The United States of America in no manner whatsoever, expressly or implied, warrants this information to be accurate and disclaims all liability for its use. Any person utilizing this document should seek competent professional advice to verify and assume responsibility for the suitability of this information to their particular situation.
An organic-rich, solid medium comprised of Baker's yeast, calcium chloride, vitamin B12 (cobalamin), and agar.
DSM strains: 276 Sporosarcina pasteurii 1 Sporosarcina pasteurii 317 Sporosarcina ureae L.E.1.1 320 Sporosarcina ureae L.E.P.4 2280 Sporosarcina ureae Gibson 229 40726 Sporosarcina ureae 2305 Sporosarcina ureae 2306 Sporosarcina ureae 2307 Sporosarcina ureae (none are sequenced)
An organic-rich, solid microbiological culture medium containing meat extract, peptone, agar, and urea.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium2.pdf 2. BACILLUS PASTEURII MEDIUM To medium 1 add 20 g/l urea before autoclaving. Do not adjust pH; pH raises to about 8 due to heat degraded urea. For sporulation enhancement add 10 mg/l MnSO4 x H2O. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium3.pdf 3. AZOTOBACTER MEDIUM Glucose 5.00 g Mannitol 5.00 g CaCl2 x 2 H2O 0.10 g MgSO4 x 7 H2O 0.10 g Na2MoO4 x 2 H2O 5.00 mg K2HPO4 0.90 g KH2PO4 0.10 g FeSO4 x 7 H2O 0.01 g CaCO3 5.00 g Agar 15.00 g Distilled water 950.00 ml Adjust pH to 7.3. Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving. Calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 375 Azomonas agilis 89 Azomonas agilis 721 Azomonas macrocytogenes 722 Azomonas macrocytogenes 376 Azorhizophilus paspali 388 Azorhizophilus paspali 391 Azorhizophilus paspali 400 Azorhizophilus paspali 2283 Azorhizophilus paspali 88 Azorhizophilus paspali 2284 Azotobacter armeniacus 282 Azotobacter beijerinckii 373 Azotobacter beijerinckii 381 Azotobacter beijerinckii 1041 Azotobacter beijerinckii 281 Azotobacter chroococcum 328 Azotobacter chroococcum 368 Azotobacter chroococcum 369 Azotobacter chroococcum 374 Azotobacter chroococcum 397 Azotobacter chroococcum 393 Azotobacter chroococcum 398 Azotobacter chroococcum 2286 Azotobacter chroococcum 2288 Azotobacter nigricans subsp. nigricans 279 Azotobacter vinelandii 332 Azotobacter vinelandii 366 Azotobacter vinelandii 382 Azotobacter vinelandii 389 Azotobacter vinelandii 390 Azotobacter vinelandii 395 Azotobacter vinelandii 399 Azotobacter vinelandii 576 Azotobacter vinelandii 720 Azotobacter vinelandii 2289 Azotobacter vinelandii 2290 Azotobacter vinelandii 85 Azotobacter vinelandii 86 Azotobacter vinelandii 87 Azotobacter vinelandii 13529 Azotobacter vinelandii 44106 Saccharomonospora cyanea NA-134
An organic-rich, solid medium containing glucose, mannitol, calcium chloride, magnesium sulfate, sodium molybdate, potassium phosphate, ferrous sulfate, calcium carbonate, and agar.
DSM strains: 277 Bacillus fastidiosus 278 Bacillus fastidiosus 280 Bacillus fastidiosus 283 Bacillus fastidiosus 324 Bacillus fastidiosus 325 Bacillus fastidiosus 326 Bacillus fastidiosus 1301 Bacillus fastidiosus 1305 Bacillus fastidiosus 72 Bacillus fastidiosus 73 Bacillus fastidiosus 76 Bacillus fastidiosus 77 Bacillus fastidiosus 78 Bacillus fastidiosus 82 Bacillus fastidiosus 83 Bacillus fastidiosus 91 Bacillus fastidiosus 1306 Bacillus sp. 1302 Bacillus sp. 1303 Bacillus sp. 1304 Bacillus sp. 43292 Mycobacterium vaccae 43113 Mycobacterium vaccae
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium6.pdf 6. ALLANTOIN MINERAL MEDIUM K2HPO4 0.8 g KH2PO4 0.2 g MgSO4 x 7 H2O 0.5 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 0.01 g MnSO4 x H2O 1.0 mg Allantoin 20.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, solid culture medium comprised of potassium phospate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, allantoin, and agar. With the mix of potassium phosphates, the pH should be about 7.4.
An organic rich, solid medium containing glucose, peptone, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium7.pdf 7. ANCYLOBACTER - SPIROSOMA MEDIUM Glucose 1.0 g Peptone 1.0 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 For DSM 27188 use washed agar and adjust to pH 5.0-5.5. © 2007 DSMZ GmbH - All rights reserved
DSM 27188 is not in StrainInfo, TaxBrowser, or www.dsmz.de.
Similar to DSMZ Medium 7, except washed agar is used instead of agar and the pH is reduced to 5.0-5.5.
Agar that has been purified/treated through the process of washing.
An organic-rich, solid medium containing glucose, peptone, yeast extract, calcium carbonate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium8.pdf 8. BACILLUS "RACEMILACTICUS" MEDIUM Glucose 5.0 g Peptone 5.0 g Yeast extract 5.0 g CaCO3 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 220, except methanol is added.
Similar to DSMZ Medium 220, except sodium chloride is increased and manganese sulfate is added.
DSM 21449 is not in www.dsmz.de, TaxBrowser, or StainInfo. DSM 21450 is not in www.dsmz.de, TaxBrowser, or StainInfo
DSMZ Medium 220.1 -< for DSM 21449 and DSM 21450
An organic rich, liquid culture medium containing casein peptone (casitone), meat extract, yeast extract, glucose, Tween 80 (polysorbate 80), potassium phosphate, sodium acetate, ammonium citrate, magnesium sulfate, and manganese sulfate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11.pdf 11. MRS MEDIUM Casein peptone, tryptic digest 10.00g Meat extract 10.00g Yeast extract 5.00g Glucose 20.00 g Tween 80 1.00g K2HPO4 2.00g Na-acetate 5.00g (NH4)2 citrate 2.00g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05g Distilled water 1000.00 ml Adjust pH to 6.2 - 6.5. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium12.pdf 12. SOIL EXTRACT MEDIUM Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121˚C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize. © 2007 DSMZ GmbH - All rights reserved
A liquid culture medium comprised of a soil extract and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium13.pdf 13. BACILLUS ACIDOCALDARIUS MEDIUM Solution A: Yeast extract 1.00g (NH4)2SO4 0.20g MgSO4 x 7 H2O 0.50g CaCl2 x 2 H2O 0.25g KH2PO4 0.60g Distilled water 500.00 ml Adjust pH to 3.0 - 4.0. Solution B: Glucose 1.00g Agar 20.00 g Distilled water 500.00 ml Sterilize solution A and B separately at 121˚C for 15 min. Cool to 50˚C combine. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of yeast extract, ammonium sulfate, magnesium sulfate, calcium chloride, potassium phosphate, glucose, and agar.
An organic-rich, liquid culture medium comprised of glucose, peptone, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium21.pdf 21. SARCINA MEDIUM Glucose 30.0g Peptone 5.0g Yeast extract 5.0 g Distilled water 100.00 ml Adjust pH to 6.0. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium25.pdf 25. RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM Yeast extract 0.25 g Mannitol 1.50 g Na-gluconate 0.50 g KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NH4Cl 0.40 g NaCl 0.40 g CaCl2 x 2 H2O 0.05 g Trace element solution SL-6 (see medium 27) 1.00 ml Distilled water 1000.00 ml Adjust pH to 4.9, then add: Fe(III) citrate solution (0.1% in H2O) 5.00 ml Biotin solution (0.002% in H2O) 1.00 ml p-Aminobenzoic acid solution (0.01% in H2O) 1.00 ml Sterilize in screw-capped bottles. Cool to room temperature; to each 100 ml medium add 0.2 ml of a sterile 10% Na2S203 solution. Incubate in the light using a tungsten lamp. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of yeast extract, mannitol, sodium gluconate, potassium phosphate, magnesium sulfate, ammonium chloride, sodium chloride, calcium chloride, trace elements, ferric citrate, biotin, p-aminobenzoic acid (4-aminobenzoic acid), and sodium thiosulfate.
Similar to DSMZ Medium 27, except the yeast extract concentration is reduced and the pH is reduced to 5.7.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium26.pdf 26. ACID RHODOSPIRILLACEAE MEDIUM Use medium 27 and reduce the yeast extract concentration to 0.2 g/l. Adjust pH to 5.7. Incubate in the light using a tungsten lamp. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28.pdf 28. PFENNIG'S MEDIUM I Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-12 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-12 B: Distilled water 1000.00 ml Na2-EDTA 3.00 g FeSO4 x 7 H2O 1.10 g CoCl2 x 6 H2O 190.00 mg MnCl2 x 2 H2O 50.00 mg ZnCl2 42.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 18.00 mg H3BO3 300.00 mg CuCl2 x 2 H2O 2.00 mg Adjust pH to 6.0. Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 7.1-7.3. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of calcium chloride, yeast extract, sodium sulfide, sodium bicarbonate, resazurin, ammonium chloride, ammonium acetate, pyruvic acid sodium salt (sodium pyruvate), dextrose (D-glucose), magnesium sulfate, potassium chloride, potassium phosphate, trace elements, vitamin B12 (cobalamin), sodium carbonate, and sulfuric acid. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28a.pdf 28a. THIORHODOCOCCUS MEDIUM supplement medium 28 with NaCl (2%), MgCl2 x 6 H2O (0.1%) and sodium thiosulfate (0.05%). Incubate at 500 to 1000 lux light intensity. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 28, except sodium chloride, magnesium chloride, and sodium thiosulfate are added.
Similar to DSMZ Medium 28, except sodium chloride, sodium thiosulfate, and magnesium chloride are added. pH is increased to 7.5
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium28b.pdf 28b. THIORHODOCOCCUS MEDIUM II Supplement Medium 28 with NaCl 1.50 % Na2S2O3 x 5 H2O 0.05 % MgCl x 6 H2O 0.25 % Adjust to pH 7.5, anaerobic incubation under nitrogen/carbon dioxide atmosphere; light (see medium 28). © 2008 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29.pdf 29. PFENNIG'S MEDIUM II Solution A: CaCl2 x 2 H2O 0.25 g Yeast extrakt 0.25 g Distilled water 460.00 ml Fill 10x 46ml in 100 ml screw-cap bottles. Bubble with N2/CO2 and autoclave 121°C 15 min. (For marine or estuarine isolates add 100.0 g NaCl to this solution and increase the MgSO4 x 7 H2O to 15.0 g). Solution B: Na2S x 9 H2O 2.00 g Distilled water 135.00 ml Prepare in a screw-cap bottle, bubble with N2 to replace air, close tightly and autoclave. Solution C: NaHCO3 1.50 g H2O 50.00 ml Bubble with CO2 and filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution D Resazurin (0,1%) 0.5 ml Distilled water 450.00 ml Autoclave in a cotton-stoppered Erlenmeyer flask with an outlet tube for medium, connected to a glass outlet at the bottom of the vessel and has, at the other end, a silicon rubber tube with a pinch cock and a bell for aseptic dispensing of the medium into bottles. Cool to room temperature under an atmosphere of N2/CO2 in an ice bath. Solution E: Ammonium chloride 0.35 g Ammonium acetate 0.25 g Pyruvic acid sodium salt 0.25 g Dextrose 0.25 g MgSO4x7H2O 0.50 g KCL 0.35 g KH2PO4 0.35 g Trace element solution SL-10 B 1.00 ml Distilled water 25 ml Filter sterilize into sterile, gas-tight, 100 ml screw-cap bottle. Solution F: Vitamin B12 0.01 g Distilled water 100.00 ml Filter sterilized Trace element solution SL-10 B: Distilled water 1000.0 ml HCl (25%) 7.7 ml FeSO4 x 7 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 300.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 24.0 mg Na2MoO4 x 2 H2O 36.0 mg Mix solution D, C and E. Bubble with CO2 in an ice bath under sterile conditions. Fill 50 ml in each bottle of solution A. Before using add 4 ml solution B and 0.1 ml solution F. Adjust the pH with filter-sterilised 1M Na2CO3 to 6.8 -7.1. Fill in sterile, N2 gassed screw-cap tubes under N2 gas. During the first 24 h, the iron of the medium precipitates in the form of black flocks. No other sediment should arise in the otherwise clear medium. Feed periodically with neutralized 3% solution of sodium sulfide to replenish sulfide and with other supplement solutions (see Ref. 3365). Neutralized sulfide solution: Distilled water 100.00 ml Na2S x 9 H2O 3.00 g The sulfide solution is prepared in a 250 ml screw-capped bottle with a butyl rubber septum and a magnetic stirrer. The solution is bubbled with nitrogen gas, closed and autoclaved for 15 min. at 121˚C. After cooling to room temperature the pH is adjusted to about 7.0 by adding of sterile 2 M H2SO4 drop-wise with a syringe without opening the bottle. Appearance of a yellow colour indicates the drop of pH to about 8. The solution should be stirred continuously to avoid precipitation of elemental sulfur. The final solution should be clear and is yellow in colour. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium29a.pdf 29a. MEDIUM FOR CHLOROBIUM FERROOXIDANS From medium 29, omit sulfide, add 10 mM bicarbonate, 10 mM FeSO4 and 2mM acetate. Adjust pH to 6.8. At 25˚C in light the incubation time is about 4 weeks. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 29, except sodium sulfide is omitted and sodium bicarbonate, ferrous sulfate, and sodium acetate are added, and the pH is decreased to 6.8.
A trace elements solution containing disodium EDTA, ferrous sulfate, cobalt chloride, manganese chloride, zinc chloride, nickel chloride, sodium molybdate, boric acid, and copper chloride.
A trace elements solution containing hydrochloric acid (hydrogen chloride), ferrous sulfate, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, and sodium molybdate.
Similar to DSMZ Medium 28, except that additional yeast extract (twice the amount) is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium39.pdf 39. PFENNIG'S MEDIUM I WITH YEAST EXTRACT To medium 28 add 0.05% yeast extract. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium38.pdf 38. CLOSTRIDIUM STICKLANDII MEDIUM Tryptone 20.00 g Yeast extract 10.00 g K2HPO4 1.04 g KH2PO4 0.68 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under N2. © 2014 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium37.pdf 37. SPIRILLUM MEDIUM Peptone (BD) 10.000 g Succinic acid 1.000 g (NH4)2SO4 1.000 g MgSO4 x 7 H20 1.000 g FeCL3 x 6 H20 0.002 g MnSO4 x H20 0.002 ml Distilled water 1000.0 ml Adjust pH to 6.8 © 2007 DSMZ GmbH - All rights reserved
A liquid, organic rich culture medium comprised of peptone, succinic acid, ammonium sulfate, magnesium sulfate, ferric trichloride, and manganese sulfate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium36.pdf 36. THIOBACILLUS THIOPARUS MEDIUM (NH4)2SO4 0.10 g K2HPO4 4.00 g KH2PO4 4.00 g MgSO4 x 7 H2O 0.10 g CaCl2 0.10 g FeCl3 x 6 H2O 0.02 g MnSO4 x H2O 0.02 g Na2S2O3 x 5 H2O 10.00 g Distilled water 1000.00 ml Dissolve all the ingredients in distilled water and adjust the pH to 6.6. Sterilize by autoclaving at 115˚C for 20 min. For solid medium add 12.00 g/l purified agar after adjustment of pH. For DSM 18181: Adjust pH of medium to 7.5 and add 1.00 g/l yeast extract. For solid medium add 20.00 g/l purified agar. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric chloride, manganese sulfate, and sodium thiosulfate.
Similar to DSMZ Medium 36, except yeast extract is added and the pH is increased to 7.5.
A minerals-salts, liquid culture medium containing ammonium chloride, potassium phosphate, magnesium chloride, calcium chloride, sodium thiosulfate, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35a.pdf 35a. THIOMONAS INTERMEDIA MEDIUM NH4Cl 0.1 g KH2PO4 3.0 g MgCl2 x 6 H2O 0.1 g CaCl2 0.1 g Na2S2O3 x 5 H2O 5.0 g Yeast extract 1.0 g Distilled water 1000.0 ml Adjust pH to 5.5-6.0 and autoclave. Add sodium thiosulfate after autoclaving from a sterile stock solution sterilized by filtration. Best growth is observed in the syneresis water of slant agar tubes (20.0 g/l agar). For DSM 22701 change amount of yeast extract to 0.5 g/l and of Na-thiosulfate to 2.5 g/l. © 2011 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 35a, except that the amounts of yeast extract and thiosulfate are reduced.
A minerals-salts, liquid culture medium containing ammonium chloride, potassium phosphate, magnesium chloride, calcium chloride, and powdered sulfur (elemental sulfur).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium35.pdf 35. ACIDITHIOBACILLUS THIOOXIDANS MEDIUM NH4Cl 0.10 g KH2PO4 3.00 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.14 g Sulfur, powdered 10.00 g Distilled water 1000.00 ml Dissolve all ingredients, except the sulfur, in distilled water and adjust the pH to 4.2, then autoclave. Place the sulfur in screw-capped tubes or bottles and sterilize by autoclaving at 112˚C for 15 min. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. For DSM 612 supplement medium with 0.10 g/l yeast extract (sterilized separately). © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 35, except yeast extract is added.
An organic-rich, liquid culture medium containing yeast extract, trypticase peptone, soy peptone, sodium acetate, sodium glutamate, thiamine hydrochloride, biotin, potassium phosphate, magnesium sulfate, and tris buffer.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium34.pdf 34. CARYOPHANON LATUM MEDIUM Yeast extract 2.00 g Trypticase (BBL) 2.00 g Soya peptone 2.00 g Na-acetate 1.00 g Na-glutamate 0.10 g Thiamine-HCl x 2 H2O 0.20 mg Biotin 0.05 mg K2HPO4 1.00 g MgSO4 x 7 H2O 0.27 g Tris/HCl-buffer 10mM, pH 7.8 1000.00 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid microbiological culture medium containing peptone, meat extract, agar, sodium bicarbonate, and sodium carbonate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium31.pdf 31. ALKALINE NUTRIENT AGAR Same as medium 1. After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7. Na-sesquicarbonate solution: NaHCO3 4.2 g Na2CO3 anhydrous 5.3 g Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium48.pdf 48. TRYPTONE THIOGLYCOLATE MEDIUM K2HPO4 5.450 g KH2PO4 1.200 g MgSO4 x 7 H2O 0.025 g CaCl2 x 2 H2O 0.015 g FeSO4 x 7 H2O 0.010 g MnCl2 x 4 H2O 2.000 mg CoCl2 x 6 H2O 2.500 mg Na2MoO4 x 2 H2O 2.500 mg Glucose 20.000 g Peptone 2.000 g Tryptone 2.000 g Yeast extract 6.000 g Na-thioglycolate 0.500 g Distilled water 1000.000 ml Adjust pH to 7.5. Dissolve the glucose in 50 ml distilled water and sterilize it separately. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese chloride, cobalt chloride, sodium molybdate, glucose, peptone, tryptone, yeast extract, and sodium thioglycolate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium49.pdf 49. C/10 MEDIUM Casitone 3.00 g CaCl2 x 2 H2O 1.36 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2 before adding agar. © 2007 DSMZ GmbH - All rights reserved
An organic rich, solid culture medium comprised of casitone, calcium chloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium40.pdf 40. PFENNIG'S MEDIUM II WITH SALT To medium 29 add 1% NaCl. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 29, except that sodium chloride is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium52.pdf 52. CLOSTRIDIUM KLUYVERI MEDIUM (MODIFIED) K-acetate 10.00 g K2HPO4 0.31 g KH2PO4 0.23 g NH4Cl 0.25 g MgSO4 x 7 H2O 0.20 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Resazurin 0.50 mg Ethanol 20.00 ml NaHCO3 2.50 g Seven vitamin solution (see medium 503) 1.00 ml L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve all ingredients (except ethanol, bicarbonate, reducing agents and vitamins) in distilled water and boil for 1 min. After cooling to room temperature under 80% N2 and 20% CO2 gas atmosphere, add ethanol, dispense in Hungate tubes and autoclave. After autoclaving, add bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas atmosphere and vitamins from an anoxic and filter-sterilized stock solution. Before inoculation, add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of medium to 6.8 – 7.0, if necessary. If reduction of medium is not complete after inoculation, add 10 – 20 mg/l sodium dithionite from a 5% w/v solution freshly prepared under N2 and filter sterilized. © 2009 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium containing potassium acetate, potassium phosphate, ammonium chloride, magnesium sulfate, trace elements, selenite-tungstate solution, yeast extract, resazurin, ehtanol, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium51.pdf 51. SPHAEROTILUS MEDIUM Beef extract (Lab Lemco, Oxoid) 5.0 g Agar (if necessary) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Prepare sterile agar slants by autoclaving. Cool the slants in a sloping position. Cover solid slants with 2 ml sterile tap water. Inoculate into the covering tap water and incubate at 20 to 25˚C for at least 48 h. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of beef extract and agar.
An organic-rich, solid culture medium comprised of casein peptone tryptic digest (casitone), yeast extract, glucose, sodium chloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf 53. CORYNEBACTERIUM AGAR Casein peptone, tryptic digest 10.0 g Yeast extract 5.0 g Glucose 5.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2 - 7.4. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54.pdf 54. GLUCOSE YEAST EXTRACT MEDIUM Glucose 20.0 g Yeast extract 10.0 g CaCO3 (light precipitate) 20.0 g Distilled water 1000.0 ml Dissolve ingredients (except carbonate, which remains solid) and sparge medium for 30 min. with 100% N2 gas. Dispense under stirring to keep carbonate suspended in anoxic vessels and autoclave. For solid medium add 17.0 g/l agar. © 2010 DSMZ GmbH - All rights reserved
DSM strains: 30201 Agrobacterium 50012 Burkholderia glathei 50014 Burkholderia glathei 50401 Burkholderia 53 Clostridium beijerinckii 6422 Clostridium beijerinckii 6423 Clostridium beijerinckii 6566 Clostridium butyricum 525 Clostridium pasteurianum DSM 525 = ATCC 6013 526 Clostridium pasteurianum 9989 Clostridium pasteurianum 523 Clostridium 3873 Pantoea cypripedii 30182 Pantoea cypripedii 30183 Pantoea cypripedii 30184 Pectobacterium atrosepticum 30185 Pectobacterium atrosepticum 30186 Pectobacterium atrosepticum 50069 Pseudomonas aeruginosa 5024 Pseudomonas aeruginosa 50259 Pseudomonas asplenii 50260 Pseudomonas cichorii 7231 Pseudomonas cichorii 50275 Pseudomonas fuscovaginae 50276 Pseudomonas marginalis 50282 Pseudomonas marginalis 50286 Pseudomonas savastanoi 80287 Pseudomonas savastanoi 50298 Pseudomonas savastanoi 50317 Pseudomonas 50291 Pseudomonas 1241 Pseudomonas syringae 1242 Pseudomonas syringae 1856 Pseudomonas syringae 10604 Pseudomonas syringae DSM 10604 50255 Pseudomonas syringae pv. atrofaciens str. DSM 50255 50256 Pseudomonas syringae 50261 Pseudomonas syringae 50277 Pseudomonas syringae 50281 Pseudomonas syringae 50292 Pseudomonas syringae 50293 Pseudomonas syringae 50302 Pseudomonas syringae 50303 Pseudomonas syringae 50312 Pseudomonas syringae 50315 Pseudomonas syringae 50335 Pseudomonas syringae 50336 Pseudomonas syringae 30200 Agrobacterium rhizogenes 1805 Sphingomonas echinoides ATCC 14820 14405 Stenotrophomonas rhizophila 50853 Xanthomonas arboricola 50854 Xanthomonas arboricola 50850 Xanthomonas axonopodis 1050 Xanthomonas campestris 1526 Xanthomonas campestris 2405 Xanthomonas campestris 2406 Xanthomonas campestris 2407 Xanthomonas campestris 3586 Xanthomonas campestris pv. campestris str. ATCC 33913 50852 Xanthomonas campestris 1220 Xanthomonas citri pv. malvacearum 50857 Xanthomonas hortorum 50858 Xanthomonas hortorum 50859 Xanthomonas hortorum 50855 Xanthomonas hyacinthi 1350 Xanthomonas sp. DSM 1350 3851 Xanthomonas
An organic-rich, liquid culture medium comprised of glucose, yeast extract, and calcium carbonate. The pKa of calcium carbonate is 9.0, therefore the pH of the solution will be about pH 9.0.
An inorganic salts solution comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium bicarbonate, and sodium chloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium58.pdf 58. BIFIDOBACTERIUM MEDIUM Casein peptone, tryptic digest 10.00 g Yeast extract 5.00 g Meat extract 5.00 g Bacto Soytone 5.00 g Glucose 10.00 g K2HPO4 2.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H20 0.05 g Tween 80 1.00 ml NaCl 5.00 g Cysteine-HCl x H2O 0.50 g Salt solution (see below) 40.00 ml Resazurin (25 mg/100ml) 4.00 ml Distilled water 950.00 ml The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml © 2008 DSMZ GmbH - All rights reserved
DSM strains: 416 Cupriavidus necator 418 Cupriavidus necator 422 Cupriavidus necator
A minerals-salts, liquid culture medium comprised of disodium hydrogen phosphate, potassium dihydrogen phosphate, ammonium chloride, magnesium sulfate, trace elements, calcium chloride, ferric ammonium citrate, and fructose. The pH of this medium should be about 7.2, given the ratio of sodium phosphate and potassium phosphate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium55.pdf 55. FRUCTOSE MINERAL MEDIUM Solution A: Na2HPO4 x 12 H2O 9.000 g KH2PO4 1.500 g NH4Cl 1.000 g MgSO4 x 7 H2O 0.200 g Trace element solution 0.300 ml SL-6 (see medium 27) Distilled water 500.000 ml Solution B: CaCl2 x 2 H2O 0.010 g Ferric ammonium citrate 0.005 g Distilled water 300.000 ml Solution C: Fructose 5.000 g Distilled water 200.000 ml Autoclave solutions 1 and 2 separately, filter sterilize solution C. Add 1.5% agar if necessary. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium56.pdf 56. TRYPTICASE STARCH AGAR Trypticase soy broth 30.0 g Yeast extract 2.0 g Soluble starch 1.0 g Agar 15.0 g Distilled water 1000.0 g © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of trypticase soy broth (here, tryptic soy agar), yeast extract, and soluble starch.
DSM strains: 22365 Aeriscardovia aeriphila 17774 Alloscardovia criceti DSM 17774 17775 Alloscardovia criceti 17776 Alloscardovia criceti 21503 Alloscardovia omnicolens DSM 21503 17244 Anaerofustis stercorihominis DSM 17244 17241 Anaerotruncus colihominis DSM 17241 22766 Bifidobacterium actinocoloniiforme DSM 22766 20083 Bifidobacterium adolescentis ATCC 15703 20086 Bifidobacterium adolescentis 20087 Bifidobacterium adolescentis DSM 20087 24849 Bifidobacterium adolescentis 26737 Bifidobacterium aesculapii 26738 Bifidobacterium aesculapii 20098 Bifidobacterium angulatum DSM 20098 = JCM 7096 20225 Bifidobacterium angulatum 20105 Bifidobacterium animalis subsp. lactis ATCC 27536 20104 Bifidobacterium animalis subsp. animalis ATCC 25527 10140 Bifidobacterium animalis subsp. lactis DSM 10140 20089 Bifidobacterium asteroides DSM 20089 20431 Bifidobacterium asteroides 23969 Bifidobacterium biavatii DSM 23969 20215 Bifidobacterium bifidum 20082 Bifidobacterium bifidum 20239 Bifidobacterium bifidum 20456 Bifidobacterium bifidum ATCC 29521 = JCM 1255 = DSM 20456 22767 Bifidobacterium bohemicum DSM 22767 19703 Bifidobacterium bombi DSM 19703 20432 Bifidobacterium boum DSM 20432 20213 Bifidobacterium breve DSM 20213 = JCM 1192 20091 Bifidobacterium breve 23973 Bifidobacterium callitrichos DSM 23973 16992 Bifidobacterium catenulatum DSM 16992 = JCM 1194 = LMG 11043 Bifidobacterium catenulatum 20224 Bifidobacterium choerinum 20434 Bifidobacterium choerinum DSM 20434 20216 Bifidobacterium coryneforme DSM 20216 20435 Bifidobacterium cuniculi DSM 20435 20084 Bifidobacterium dentium 20221 Bifidobacterium dentium 20436 Bifidobacterium dentium JCM 1195 = DSM 20436 20093 Bifidobacterium gallicum DSM 20093 = LMG 11596 20214 Bifidobacterium indicum LMG 11587 = DSM 20214 21854 Bifidobacterium kashiwanohense JCM 15439 = DSM 21854 28807 Bifidobacterium sp. LMC 13 20088 Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088 20090 Bifidobacterium longum subsp. infantis 20090 Bifidobacterium longum subsp. infantis 20218 Bifidobacterium longum subsp. infantis 20097 Bifidobacterium longum subsp. longum 20219 Bifidobacterium longum subsp. longum 20211 Bifidobacterium longum subsp. suis DSM 20211 20220 Bifidobacterium magnum 20222 Bifidobacterium magnum DSM 20222 6492 Bifidobacterium merycicum DSM 6492 6493 Bifidobacterium merycicum 6494 Bifidobacterium merycicum 20102 Bifidobacterium minimum DSM 20102 21395 Bifidobacterium mongoliense DSM 21395 27321 Bifidobacterium moukalabense DSM 27321 20438 Bifidobacterium pseudocatenulatum DSM 20438 = JCM 1200 = LMG 10505 20439 Bifidobacterium pseudocatenulatum 20092 Bifidobacterium pseudolongum subsp. globosum DSM 20092 20094 Bifidobacterium pseudolongum subsp. pseudolongum 20095 Bifidobacterium pseudolongum subsp. pseudolongum 20099 Bifidobacterium pseudolongum subsp. pseudolongum DSM 20099 22366 Bifidobacterium psychraerophilum DSM 22366 20433 Bifidobacterium pullorum DSM 20433 23975 Bifidobacterium reuteri DSM 23975 6489 Bifidobacterium ruminantium DSM 6489 6490 Bifidobacterium ruminantium 6491 Bifidobacterium ruminantium 6531 Bifidobacterium saeculare DSM 6531 = LMG 14934 6532 Bifidobacterium saeculare 6533 Bifidobacterium saeculare 23967 Bifidobacterium saguini DSM 23967 13734 Bifidobacterium scardovii JCM 12489 = DSM 13734 23968 Bifidobacterium stellenboschense 20096 Bifidobacterium subtile DSM 20096 17755 Bifidobacterium thermacidophilum subsp. porcinum DSM 17755 15837 Bifidobacterium thermacidophilum subsp. thermacidophilum DSM 15837 Bifidobacterium thermophilum 20209 Bifidobacterium thermophilum 20210 Bifidobacterium thermophilum DSM 20210 20212 Bifidobacterium thermophilum DSM 20212 17777 Bifidobacterium tsurumiense DSM 17777 17778 Bifidobacterium tsurumiense 17779 Bifidobacterium tsurumiense 20653 Lactobacillus aviarius subsp. araffinosus DSM 20653 20656 Lactobacillus aviarius subsp. araffinosus 20654 Lactobacillus aviarius subsp. aviarius 20655 Lactobacillus aviarius subsp. aviarius DSM 20655 20584 Lactobacillus crispatus DSM 20584 = JCM 1185 20461 Megasphaera cerevisiae 20462 Megasphaera cerevisiae DSM 20462 16981 Megasphaera paucivorans 17042 Megasphaera sueciensis 21655 Mobiluncus curtisii subsp. holmesii ATCC 35242 10105 Parascardovia denticolens DSM 10105 = JCM 12538 10106 Parascardovia denticolens 24661 Pectinatus brassicae 20466 Pectinatus cerevisiiphilus 20467 Pectinatus cerevisiiphilus 20762 Pectinatus cerevisiiphilus 20465 Pectinatus frisingensis 16980 Pectinatus haikarae 20764 Pectinatus sp. DSM 20764 24742 Pseudoscardovia radai 24744 Pseudoscardovia suis 16840 Roseburia faecis M72/1 16839 Roseburia hominis A2-183 10107 Scardovia inopinata 10108 Scardovia inopinata 20757 Selenomonas lacticifex 18934 Sharpea azabuensis DSM 18934 17536 Streptococcus castoreus DSM 17536 7200 Zymophilus paucivorans 7201 Zymophilus paucivorans 20756 Zymophilus paucivorans 7198 Propionispira raffinosivorans 7199 Propionispira raffinosivorans 20765 Propionispira raffinosivorans DSM 20765
An organic-rich, liquid culture medium comprised of casein peptone, tryptic digest (casitone), yeast extract, meat extract, Bacto soytone (soy peptone), glucose, potassium phosphate, magnesium sulfate, manganese sulfate, tween 80 (polysorbate 80), sodium chloride, cysteine hydrochloride, salt solution and resazurin. Prepared under an atmosphere of dinitrogen and carbon dioxide.
An organic-rich, liquid culture medium containing tryptone, meat extract, yeast extract, tween 80 (polysorbate 80), sodium acetate, magnesium sulfate, magnesium sulfate, and casamino acids.
DSM strains: 20253 Lactobacillus rennini DSM 20253 20254 Lactobacillus rennini
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium57.pdf 57. AAM MEDIUM Tryptone 5.0 g Meat extract 5.0 g Yeast extract 7.0 g Tween 80 1.0 ml Na-acetate 2.5 g MgSO4 x 7 H2O 200.0 mg MnSO4 x H2O 50.0 mg Casamino acids 7.0 g Distilled water 1000.0 ml Adjust pH to 5.4. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 20252 Oenococcus oeni DSM 20252 = AWRIB129 20255 Oenococcus oeni 20257 Oenococcus oeni
An organic-rich, liquid culture medium comprised of casein peptone tryptic digest (casitone), yeast extract, glucose, fructose, magnesium sulfate, manganese sulfate, ammonium citrate, tween 80 (polysorbate 80), filtered tomato juice, and cysteine hydrochloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium59.pdf 59. LEUCONOSTOC OENOS MEDIUM Casein peptone, tryptic digest 10.00 g Yeast extract 5.00 g Glucose 10.00 g Fructose 5.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05 g (NH4) citrate 3.50 g Tween 80 1.00 ml Tomato juice, filtered 100.00 ml Cysteine-HCl x H2O 0.50 g Distilled water 900.00 ml Adjust pH to 4.8. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of magnesium sulfate, ammonium sulfate, ferrous ammonium sulfate, sodium molybdate, sodium selenite, tryptone, yeast extract, resazurin, potassium phosphate, glucose, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of carbon dioxide and dinitrogen.
DSM strains: 521 Moorella thermoacetica 11768 Moorella thermoacetica
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium60.pdf 60. CLOSTRIDIUM THERMOACETICUM MEDIUM Solution A: MgSO4 x 7 H2O 0.10 g (NH4)2SO4 0.50 g Fe(NH4)2(SO4)2 0.04 g Na2MoO4 x 2 H2O 2.40 mg Na2SeO3 x 5 H2O 0.15 mg Tryptone 5.00 g Yeast extract 5.00 g Resazurin 0.50 mg Distilled water 800.00 ml Solution B: K2HPO4 7.00 g KH2PO4 4.50 g Distilled water 50.00 ml Solution C: Glucose 18.00 g Distilled water 50.00 ml Solution D: NaHCO3 10.00 g Distilled water 100.00 ml Solution E: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Prepare solution A under 100% CO2 gas atmosphere. Distribute and autoclave under same gas atmosphere. Autoclave separately solutions B, C, E and F under 100% N2, and solution D under 100% CO2 gas atmosphere. After sterilization combine all solutions aseptically. Adjust pH of the complete medium to 6.9 if necessary. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, sucrose, yeast extract, ferrous sulfate, sodium sulfite, sodium thiosulfate, and resazurin. Prepared under an atmosphere of dinitrogen.
Clostridium thermohydrosulfuricum medium
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium61.pdf 61. CLOSTRIDIUM THERMOHYDROSULFURICUM MEDIUM Tryptone 10.00 g Sucrose 10.00 g Yeast extract 2.00 g FeSO4 x 7 H2O 0.20 g Na2SO3 0.20 g Na2S2O3 x 5 H2O 0.08 g Resazurin 1.00 mg Distilled water 1000.00 ml Dissolve ingredients (except thiosulfate), adjust pH to 6.8 - 7.5 and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add thiosulfate from a sterile anoxic stock solution prepared under N2. For DSM 8686 and DSM 8690 replace sucrose with D-xylose as substrate and adjust pH of medium to 5.2. For DSM 15493 replace sucrose with 3.0 g/l D-maltose. © 2014 DSMZ GmbH - All rights reserved
DSM strains: 2246 Thermoanaerobacter ethanolicus JW 200 12299 Thermoanaerobacter siderophilus SR4 15493 Thermoanaerobacter sp. 518-21 567 Thermoanaerobacter thermohydrosulfuricus 568 Thermoanaerobacter thermohydrosulfuricus 569 Thermoanaerobacter thermohydrosulfuricus 570 Thermoanaerobacter thermohydrosulfuricus 2247 Thermoanaerobacter thermohydrosulfuricus 7021 Thermoanaerobacter thermohydrosulfuricus 7022 Thermoanaerobacter thermohydrosulfuricus 26960 Thermoanaerobacter thermohydrosulfuricus WC1 14765 Thermoanaerobacterium bryantii 8686 Thermoanaerobacterium 8690 Thermoanaerobacterium 571 Thermoanaerobacterium thermosaccharolyticum DSM 571 572 Thermoanaerobacterium thermosaccharolyticum 573 Thermoanaerobacterium thermosaccharolyticum 869 Thermoanaerobacterium thermosaccharolyticum
DSMZ Medium 61.1 -< for DSM 8686 and DSM 8690
Similar to DSMZ Medium 61, except that sucrose is omitted, D-xylose is added, and the pH is reduced to 5.2.
DSM 8686 is Thermoanaerobacterium sp. DSM 8690 is Thermoanaerobacterium sp. Neither strains are in TaxBrowser
DSMZ 15493 is Thermoanaerobacter sp. 518-21
Similar to DSMZ Medium 61, except that sucrose is omitted and D-maltose (beta-maltose) is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63.pdf 63. DESULFOVIBRIO MEDIUM (FRESHWATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 12838 supplement medium with 3.0 g/l meat extract. © 2015 DSMZ GmbH - All rights reserved
DSM 12838 is Desulfomicrobium orale DSM 12838
Similar to DSMZ Medium 63, except that meat extract is added.
DSM strains: 24233 Desulfobaculum xiamenense 5918 Desulfomicrobium apsheronum 1742 Desulfomicrobium baculatum 1743 Desulfomicrobium baculatum 2555 Desulfomicrobium baculatum 4028 Desulfomicrobium baculatum DSM 4028 10707 Desulfomicrobium escambiense DSM 10707 4194 Desulfomicrobium macestii 12838 Desulfomicrobium orale DSM 12838 12927 Desulfomicrobium 15374 Desulfomicrobium 12929 Desulforhopalus 13351 Desulfosporosinus auripigmenti 765 Desulfosporosinus orientis DSM 765 7439 Desulfosporosinus orientis 11792 Desulfotomaculum australicum DSM 11792 4024 [Desulfotomaculum] guttoideum DSM 4024 6115 Desulfotomaculum kuznetsovii DSM 6115 12396 Desulfotomaculum luciae 574 Desulfotomaculum nigrificans DSM 574 575 Desulfotomaculum nigrificans 7434 Desulfotomaculum nigrificans 7435 Desulfotomaculum nigrificans 7436 Desulfotomaculum nigrificans 7437 Desulfotomaculum nigrificans 7438 Desulfotomaculum nigrificans 7453 Desulfotomaculum nigrificans 12395 Desulfotomaculum putei DSM 12395 2154 Desulfotomaculum ruminis DSM 2154 7452 Desulfotomaculum ruminis 6711 Desulfotomaculum 7440 Desulfotomaculum 7441 Desulfotomaculum 7442 Desulfotomaculum 7443 Desulfotomaculum 14812 Desulfotomaculum 15631 Desulfotomaculum 5813 Desulfotomaculum thermoacetoxidans 6193 Desulfotomaculum thermobenzoicum subsp. thermobenzoicum 2603 Desulfovibrio africanus subsp. africanus DSM 2603 23860 Desulfovibrio africanus subsp. uniflagellum 6133 Desulfovibrio alcoholivorans 12254 Desulfovibrio aminophilus DSM 12254 21064 Desulfovibrio arcticus 6830 Desulfovibrio burkinensis 11391 Desulfovibrio cuneatus DSM 11391 11392 Desulfovibrio cuneatus 642 Desulfovibrio desulfuricans subsp. desulfuricans DSM 642 1924 Desulfovibrio desulfuricans subsp. desulfuricans 4369 Desulfovibrio desulfuricans subsp. desulfuricans 6946 Desulfovibrio desulfuricans subsp. desulfuricans 9104 Desulfovibrio desulfuricans subsp. desulfuricans 12129 Desulfovibrio desulfuricans subsp. desulfuricans 3604 Desulfovibrio fructosivorans JJ 15450 Desulfovibrio idahonensis DSM 15450 15451 Desulfovibrio idahonensis 15121 Desulfovibrio indonesiensis 11275 Desulfovibrio intestinalis DSM 11275 11393 Desulfovibrio litoralis DSM 11393 6739 Desulfovibrio longus DSM 6739 1925 Desulfovibrio oxamicus 16681 Desulfovibrio paquesii DSM 16681 4141 Desulfovibrio simplex 496 Desulfovibrio 6605 Desulfovibrio 8712 Desulfovibrio 9266 Desulfovibrio 12803 Desulfovibrio 15744 Desulfovibrio 5308 Desulfovibrio termitidis HI1 11274 Desulfovibrio termitidis 15375 Desulfovibrio vulgaris 644 Desulfovibrio vulgaris str. Hildenborough 2119 Desulfovibrio vulgaris 6617 Desulfovibrio vulgaris 6618 Desulfovibrio vulgaris 6619 Desulfovibrio vulgaris 6621 Desulfovibrio vulgaris 6622 Desulfovibrio vulgaris 6623 Desulfovibrio vulgaris
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen.
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium acetate, sodium pyruvate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63a.pdf 63a. DESULFOTOMACULUM SP. MEDIUM I Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-acetate 2.0 g Na-pyruvate 5.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 6.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. Check medium pH after autoclaving and adjust to 6.5 - 7.0, if necessary. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 63, except that the medium is made up in filtered aged seawater.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium63b.pdf 63b. DESULFOVIBRIO MEDIUM (SEAWATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Filtered aged sea water 980.0 ml Alternatively to filtered aged sea water bottled water from Biomaris GmbH supplemented with 15.0 g/l NaCl can be used. Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of glucose, yeast extract, calcium carbonate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium54a.pdf 54a. REACTIVATION WITH LIQUID MEDIUM 545 Rehydrate and grow lyophilized cells from the ampoule in liquid medium 54 or 545. Subsequent subculturing may be carried out in liquid medium or on agar plates medium 54. 54. GLUCOSE YEAST EXTRACT AGAR Glucose 20.0 g Yeast extract 10.0 g CaCO3 (light precipitate) 20.0 g Agar, if required 17.0 g Distilled water 1000.0 ml 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. The same as Caso Bouillon (Merck or Oxoid, Germany). © 2007 DSMZ GmbH - All rights reserved
Undefined inorganic chemical mixture, derived from the filtering of natural seawater.
A blood medium ingredient comprised of whole blood, which has not been treated or altered.
Microbiological medium ingredient, derived from chicken egg, where whole chicken eggs have been dried.
An artificial seawater made using water and Instant Ocean® sea salt.
microbiological medium ingredient, derived from chicken egg
An undefined organic chemical mixture, derived from the eggs of chickens (Gallus gallus). Used for the cultivation of microorganisms.
An undefined organic chemical mixture comprised of dried yeast cells Saccharomyces cerevisiae), used to support the growth of microorganisms.
An organic-rich, liquid culture medium comprised of potassium hydroxide, potassium phosphate, uric acid, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, selenite-tungstate solution, yeast extract, sodium thioglycolate, sodium bicarbonate, and resazuring. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSM strains: 604 [Clostridium] acidurici 9a 10158 Gottschalkia acidurici 10159 Gottschalkia acidurici 10160 Gottschalkia acidurici 10161 Gottschalkia acidurici 605 Clostridium cylindrosporum DSM 605 10157 Clostridium cylindrosporum 10162 Clostridium cylindrosporum 10163 Clostridium cylindrosporum 10164 Clostridium cylindrosporum 1384 Clostridium purinilyticum 10156 Clostridium purinilyticum 1989 Eubacterium angustum 3244 Gallicola barnesae
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium76.pdf 76. CLOSTRIDIUM ACIDIURICI MEDIUM KOH 0.67 g K2HPO4 0.91 g Uric acid 2.00 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 15.00 mg FeSO4 x 7 H2O 6.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Na-thioglycolate 0.50 g NaHCO3 5.00 g Resazurin 1.00 mg Distilled water 1000.00 ml First dissolve KOH and K2HPO4 in water, then add uric acid and boil until the acid is dissolved. Cool medium to room temperature under 100% N2 gas atmosphere and add all other substances, except thioglycolate and bicarbonate. Dispense under same gas atmosphere into culture vessels and autoclave for 15 min at 121˚C. Then add thioglycolate (stock solution, autoclaved separately) and sodium bicarbonate (filter-sterilized stock solution prepared under 80% N2 and 20% CO2 gas atmosphere) and adjust pH of complete medium to 7.0 - 7.5. © 2007 DSMZ GmbH - All rights reserved
Filtered seawater that has been aged (kept in the dark for 2-4 weeks).
An organic rich, liquid culture medium comprised of infusion from fresh liver (liver extract), peptone, potassium phosphate, and extracted liver tissue (dissected liver tissue).
DSM strains: 601 Clostridium baratii 602 Clostridium baratii 46280 Clostridium paraputrificum 599 Clostridium sardiniense 600 Clostridium sardiniense 46282 Clostridium 46278 Clostridium sporogenes 46279 Clostridium sporogenes
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium77.pdf 77. LIVER BROTH (Oxoid CM 77) Prepare the medium according to directions on the bottle under 100% N2 gas atmosphere. © 2015 DSMZ GmbH - All rights reserved
http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0077&org=3&c=UK&lang=EN LIVER BROTH Code: CM0077 a liquid medium, containing liver particles, for the examination of foods for saccharolytic or putrefactive mesophilic and thermophilic anaerobes Typical Formula* gm/litre Infusion from fresh liver 23.0 Peptone 10.0 Potassium phosphate 1.0 Extracted liver tissue 30.0 pH 6.8 ± 0.2
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium74.pdf 74. THERMUS THERMOPHILUS MEDIUM Yeast extract (BD Difco) 4.0 g Proteose peptone Nr. 3 (BD Difco) 8.0 g NaCl 2.0 g Distilled water 1000.0 ml Adjust pH to 7.0. The original medium description specifies polypeptone, which appears to be no longer available. © 2012 DSMZ GmbH - All rights reserved
DSM strains: 22914 Pseudoxanthomonas taiwanensis DSM 22914 16200 Thermus kawarayensis JCM 12314 579 Thermus thermophilus HB8
An organic rich, liquid culture medium comprised of yeast extract, proteose peptone, and sodium chloride.
DSM strains: 587 Acinetobacter 588 Acinetobacter
An organic-rich, liquid culture medium containing trypticase peptone, phytone peptone, sodium chloride, potassium phosphate, and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium75.pdf 75. TRYPTICASE PHYTONE MEDIUM Trypticase peptone 17.0 g Phytone peptone 3.0 g NaCl 5.0 g K2HPO4 2.5 g Glucose 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium73.pdf 73. MEDIUM FOR HALOPHILIC BACILLI Casamino acids 10.0 g Yeast extract 10.0 g NaCl 100.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic rich, liquid culture medium comprised of casamino acids, yeast extract, and sodium chloride.
Thiobacillus ferrooxidans medium with ferrous sulfate
Acidithiobacillus ferrooxidans medium (ferrous sulfate)
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium70.pdf 70. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (FERROUS SULFATE) KH2PO4 0.4 g MgSO4 x 7 H2O 0.4 g (NH4)2SO4 0.4 g FeSO4 x 7 H2O 33.3 g 0.1 N H2SO4 1000.0 ml Adjust pH to 1.4 with sulfuric acid. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 583 Acidithiobacillus ferrooxidans
A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium sulfate, ferrous sulfate and sulfuric acid.
DSM strains: 14366 Acidithiobacillus albertensis 584 Acidithiobacillus ferrooxidans 622 Acidithiobacillus thiooxidans 14887 Acidithiobacillus thiooxidans ATCC 19377
A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium sulfate, calcium chloride, and sodium thiosulfate.
Thiobacillus ferrooxidans medium with thiosulfate
Acidithiobacillus ferrooxidans medium (thiosulfate)
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium71.pdf 71. ACIDITHIOBACILLUS FERROOXIDANS MEDIUM (THIOSULFATE) KH2PO4 3.00 g MgSO4 x 7 H2O 0.50 g (NH4)2SO4 3.00 g CaCl2 x 2 H2O 0.25 g Na2S2O3 x 5 H2O 5.00 g Distilled water 1000.00 ml Prepare the medium without thiosulfate, adjust pH to 4.4 - 4.7 and autoclave at 121˚C for 15 min. Sterilize thiosulfate separately by filtration and add after autoclaving. For DSM 585 replace thiosulfate with 5.00 g/l tetrathionate (K2S4O6) added from a stock solution sterilized by filtration. For DSM 14366: Add 10.0 mg/l FeSO4 x 7 H2O. The final pH of the medium should be 4.4. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 71, except that thiosulfate is omitted and potassium tetrathionate added.
DSM 585 is Acidithiobacillus ferrooxidans. Straininfo.net claims that no sequences found for this strain. Strain not in TaxBrowser.
DSM 14366 is Acidithiobacillus albertensis
Similar to DSMZ Medium 71, except that ferrous sulfate is added.
DSM strains: 582 Paracoccus versutus 506 Starkeya novella DSM 506
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium69.pdf 69. THIOBACILLUS NOVELLUS MEDIUM Solution A: Na2HPO4 x 12 H2O 10.60 g KH2PO4 1.50 g NH4Cl 0.30 g Yeast extract 0.30 g Phenol red 2.00 mg Distilled water 900.00 ml Solution B: MgSO4 x 7 H2O 0.10 g Distilled water 50.00 ml Solution C: Trace element solution (see below) 5.00 ml Solution D: Na2S2O3 x 5 H2O 5.00 g Distilled water 50.00 ml Solutions A, B, C, D are sterilized separately by autoclaving and mixed when cold. For agar medium add 1.5% agar (Difco Bacto) to solution A. Adjust pH to 8.5 with sterile 0.5 N NaOH. Trace element solution (Vishniac and Santer, 1957): Na2-EDTA 50.00 g ZnSO4 x 7 H2O 22.00 g CaCl2 x 2 H2O 5.54 g MnCl2 x 4 H2O 5.06 g FeSO4 x 7 H2O 5.00 g (NH4)6Mo7O24 x 4 H2O 1.10 g CuSO4 x 5 H2O 1.57 g CoCl2 x 6 H2O 1.61 g Distilled water 1000.00 ml Adjust pH to 6.0 with KOH. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium containing sodium phosphate, potassium phosphate, ammonium chloride, yeast extract, phenol red, mangensium sulfate, trace elements, and sodium thiosulfate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium68.pdf 68. THIOBACILLUS NEAPOLITANUS MEDIUM KH2PO4 4.0 g K2HPO4 4.0 g MgSO4 x 7 H2O 0.8 g NH4Cl 0.4 g Trace element solution (see medium 69) 5.0 ml Na2S2O3 x 5 H2O 10.0 g Bromocresol purple (saturated aqueous solution) 0.5 ml Distilled water 1000.0 ml Adjust pH to 6.6 - 7.0. Sterilize Na2S2O3 separately in 100 ml of distilled water. For agar medium use 1.5% Difco Bacto Agar. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 581 Halothiobacillus neapolitanus 15147 Halothiobacillus neapolitanus 16832 Halothiobacillus neapolitanus
A minerals-salts, liquid culture medium comprised of potassium phosphate, magnesium sulfate, ammonium chloride, trace elements, sodium thiosulfate, and bromcresol purple.
DSM strains: 3685 Chitinophaga arvensicola 2588 Chitinophaga pinensis DSM 2588 2589 Chitinophaga pinensis 784 Chondromyces crocatus 14714 Corallococcus coralloides 14687 Corallococcus coralloides 14688 Corallococcus exiguus 14696 Cystobacter badius 14738 Cystobacter gracilis 14753 Cystobacter gracilis 14754 Cystobacter gracilis 14755 Cystobacter gracilis 14771 Cystobacter gracilis 14756 Cystobacter miniatus 14751 Cystobacter minus 14752 Cystobacter minus 14772 Cystobacter minus 14758 Cystobacter violaceus Cb vi76 14759 Cystobacter violaceus 3656 Cytophaga 3657 Cytophaga 2063 Flavobacterium hydatis 2064 Flavobacterium johnsoniae UW101 6368 Flavobacterium pectinovorum 3660 Flavobacterium psychrophilum DSM 3660 1811 Flavobacterium saccharophilum 425 Flavobacterium sp. DSM 425 4001 Flavobacterium succinicans 4002 Flavobacterium succinicans 4003 Flavobacterium succinicans 21789 Flavobacterium swingsii DSM 21789 3661 Flavobacterium xanthum 6792 Flavobacterium johnsoniae 6793 Flexibacter flexilis DSM 6793 3098 Flexibacter 785 Herpetosiphon aurantiacus DSM 785 6205 Herpetosiphon aurantiacus 6206 Herpetosiphon aurantiacus 7119 Herpetosiphon geysericola 589 Herpetosiphon 6207 Herpetosiphon 11115 Hymenobacter chitinivorans 11117 Hymenobacter ocellatus 2044 Lysobacter antibioticus 2045 Lysobacter antibioticus 2043 Lysobacter enzymogenes 6980 Lysobacter gummosus 3655 Lysobacter 14740 Melittangium lichenicola 14675 Myxococcus stipitatus DSM 14675 14676 Myxococcus stipitatus 14700 Myxococcus stipitatus 4946 Myxococcus virescens 6796 Myxococcus xanthus 6797 Myxococcus xanthus 53668 Sandaracinus amylolyticus 2582 Sphingobacterium spiritivorum ATCC 33300 14737 Stigmatella hybrida 7029
An organic-rich, solid culture medium containing casitone, calcium chloride, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium67.pdf 67. CY-AGAR Casitone 3.00 g CaCl2 x 2 H2O 1.36 g Yeast extract 1.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of glucose, yeast extract, malt extract, calcium carbonate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf 65. GYM STREPTOMYCES MEDIUM Glucose 4.0 g Yeast extract 4.0 g Malt extract 10.0 g CaCO3 2.0 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 7.2 before adding agar. Delete CaCO3 if liquid medium is used. © 2007 DSMZ GmbH - All rights reserved
An undefined organic chemical mixture, derived from dissected (physically extracted) liver tissue of an animal (Mammalia).
trace elements solution - Vishniac and Santer 1957
A trace elements solution comprised of disodium EDTA, zinc sulfate, calcium chloride, manganese chloride, ferrous sulfate, ammonium molybdate, copper sulfate, and cobalt chloride.
A papaic digest of soybean (Hordeum vulgare) meal, used for the culturing of microorganisms. Phytone™ Peptone is a trademark of BBL/Difco Labs.
From BD Bionutrients Technical Manual (3rd edition revised): "Many of these media utilize peptones of known derivation, such as Trypticase™ Peptone, a pancreatic digest of casein, and Phytone™ Peptone, a papaic digest of soybean meal."
DSM 5566 is Clostridium novyi B str. ATCC 27606
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78.pdf 78. CHOPPED MEAT MEDIUM Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. For DSM 5566 add to the autoclaved medium 1 g/l NaHCO3 from a sterile, anoxic stock solution (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Adjust final pH of medium to 7.2. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.00 ml Vitamin K1 or Vitamin K3 solution (see below) 0.20 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2011 DSMZ GmbH - All rights reserved
Similar to DSM Medium 78, except that sodium bicarbonate is added. Prepared under an atmosphere of dinitrogen and carbon dioxide. The pH is increased to 7.2.
A vitamin solution containing vitamin B12 (cobalamin), pantothenic acid, riboflavin, pyridoxamine hydrochloride, biotin, folic acid, nicotinic acid, nicotine amide (nicotinamide), alpha-lipoic acid (lipoic acid), p-aminobenzoic acid (4-aminobenzoic acid), and thiamine hydrochloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium78a.pdf 78a. CHOPPED MEAT MEDIUM FOR TREPONEMA SP. To the ready medium 78, add 50 ml/l amino acid solution and 5 ml/l vitamin solution. These solutions must not be prepared under anaerobic conditions. Amino acid solution (filter sterilized): L-Histidine 0.6 g L-Serine 0.5 g L-Glutamine 0.7 g Distilled water 50.0 ml Vitamin solution: Vitamin B12 50 mg Pantothenic acid 50 mg Riboflavin 50 mg Pyridoxamine-HCl 10 mg Biotin 20 mg Folic acid 20 mg Nicotinic acid 25 mg Nicotine amide 25 mg α-lipoic acid 50 mg p-aminobenzoic acid 50 mg Thiamine-HCl x 2 H2O 50 mg Distilled water 1000 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved
An artificial (synthetic) seawater comprised of sodium chloride, magnesium chloride, sodium sulfate, calcium chloride, potassium chloride, potassium bromide, boric acid, sodium silicate, strontium chloride, sodium fluoride, ammonium nitrate, and ferric phosphate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium79.pdf 79. LEUCOTHRIX MEDIUM Tryptone 10.00 g Synthetic sea water 1000.00 ml Synthetic sea water: NaCl 24.00 g MgCl2 x 6 H2O 11.00 g Na2SO4 4.00 g CaCl2 x 6 H2O 2.00 g KCl 0.70 g KBr 0.10 g H3BO3 0.03 g NaSiO3 x 9 H2O 5.00 mg SrCl2 x 6 H2O 0.04 g NaF 3.00 mg NH4NO3 2.00 mg Fe3PO4 x 4 H2O 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1, except horse serum is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium302.pdf 302. NUTRIENT BROTH WITH 10% HORSE SERUM To autoclaved medium 1 add 100 ml/l of sterile horse serum. © 2007 DSMZ GmbH - All rights reserved
A defined organic solution containing the following amino acids: L-histidine, L-serine, and L-glutamine.
A soil extract made from garden soil. Prepared by seiving garden soil through a coarse sieve, adding distilled water, and autoclaving.
standard vitamin solution for DSMZ Medium 81
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium81.pdf 81. MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3) Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 915.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solutions A, B, C are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with 5.0 ml filter-sterilized standard vitamin solution (see below) and 10.0 ml filter-sterilized 5% NaHCO3 (pH 7-8).The final pH of this medium should be 6.8 without adjustment. For chemolithotrophic growth incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 60% H2 and 28% N2. For heterotrophic growth supplement the mineral medium with an appropriate carbon source (0.2% carbohydrate or 0.1% organic acid). For growth on nitrogen-free medium, omit NH4Cl and incubate the culture under an atmosphere of 2% (v/v) O2, 10% CO2, 10% H2 and 78% N2 or heterotrophically under 2% (v/v) O2 and 98% N2. For more details see Ref. 1515 and Ref. 3363. For DSM 21436 adjust pH to 5.0 and use a gas atmosphere of 10% O2, 10% CO2, 40% H2 and 40% N2 with an overpressure of 2 bar. Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 © 2011 DSMZ GmbH - All rights reserved
A vitamin solution comprised of riboflavin, thiamine hydrochloride, nicotinic acid, pyridoxine hydrochloride, calcium pantothenate, biotin, folic acid, and vitamin B12 (cobalamin).
Wikipedia: Rolled oats Rolled oats are traditionally oat groats that have been de-husked, steamed and then rolled into flat flakes under heavy rollers before being stabilized by being lightly toasted. The oat, like the other cereals, has a hard, inedible outer husk that must be removed before the grain can be eaten. After the outer husk (or chaff) has been removed from the still bran-covered oat grains, the remainder is called oat groats. Oat groats are a whole grain that can be used as a breakfast cereal; various forms of oatmeal and rolled oats, and pinhead oats are cooked to make porridge.[1] Steel-cut oats (pinhead oatmeal) are oat groats that have been chopped into smaller pieces before any steaming and thus retain bits of the bran layer. Since the bran layer, though nutritious, makes the grains tough to chew and contains an enzyme that can cause the oats to go rancid, raw oat groats are often further steam-treated to soften them for a quicker cooking time (modern "quick oats") and to denature the enzymes for a longer shelf life.
Undefined organic chemical mixture comprised of de-husked oat (Avena sativa) seed that has been crushed by rolling under a heavy roller and toasted.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87a.pdf 87a. CHLOROFLEXUS AGGREGANS MEDIUM To medium 87 add 1.0 ml/litre sterile vitamin solution CA. Vitamin solution CA Distilled water 100.0 ml Nicotinic acid 100.0 mg Thiamine-HCl x 2 H2O 100.0 mg Biotin 5.0 mg p-Aminobenzoic acid 50.0 mg Vitamin B12 1.0 mg Ca-pantothenate 50.0 mg Pyridoxine-HCl 50.0 mg Folic acid 50.0 mg Na3-EDTA 200.0 mg Adjust pH to 7.5. The solution is filter-sterilized. © 2007 DSMZ GmbH - All rights reserved
A vitamin solution comprised of nicotinic acid, thiamine hydrochloride, biotin, p-aminobenzoic acid (4-aminobenzoic acid), vitamin B12 (cobalamin), calcium pantothenate, pyridoxine hydrochloride, folic acid, and trisodium EDTA.
DSM strains: 1053 Methanothermobacter thermautotrophicus str. Delta H 6216 Methanoculleus bourgensis
A minerals-salts, liquid culture medium comprised of sodium carbonate, ammonium sulfate, sodium chloride, potassium phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, resazurin, vitamin solution, trace elements solution, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide.
(note this media recipe is not in the current list of media recipes at the DSMZ). From: Handbook of Microbiological Media, 3rd edition. By Ronald M. Atlas. pp. 1056. Composition per liter: sodium carbonate, 4.0g ammonium sulfate, 1.5g sodium chloride, 0.6g potassium dihydrogen phosphate, 0.3g potassium dibasic phosphate, 0.15g magnesium sulfate heptahydrate, 0.12g calcium chloride dihyrate, 0.08g iron sulfate heptahydrate, 4.0mg resazurin,1.0mg vitamin solution, 10.0 mL trace elements solution, 10.0 mL L-cysteine solution, 10.0 mL sodium sulfide nonahydrate solution, 10.0 mL pH 7.2 ± 0.2 at 25˚C. Vitamin solution Composition per liter: pyridoxine hydrochloride, 10.0mg thiamine hydrochloride dihydrate, 5.0 mg riboflavine, 5.0 mg nicotinic acid, 5.0 mg calcium pantothenate, 5.0 mg biotin, 2.0 mg folic acid, 2.0 mg p-aminobenzoic acid, 1.0 mg vitamin B12, 0.01 mg Preparation of Vitamin Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Filter sterilize. Trace elements solution Composition per liter: magnesium sulfate heptahydrate, 6.2g sodium chloride, 1.0g disodium EDTA, 0.64g manganese sulfate tetrahydrate, 0.55g zinc sulfate heptahydrate, 0.18g cobalt chloride hexahydrate, 0.17g calcium chloride dihydrate, 0.13g iron sulfate heptahydrate, 0.1g copper sulfate, 0.05g nickel chloride hexahydrate, 0.025g potassium aluminum sulfate dodecadydrate, 0.018g sodium molybdate tetrahydrate, 0.011g boric acid, 0.01g Preparation of Trace Elements Solution: Add Na2-EDTA to 500.0 mL distilled/deionized water. Mix thoroughly. Add other components and bring volume to 1.0 L with distilled/deionized water. Mix thoroughly. Sparge with 80% dihydrogen and 20% carbon dioxide. Autoclave for 15 min at 15 psi pressure-121˚C. Sodium sulfide solution Composition per 10.0 mL: sodium sulfide nonahydrate, 1.5g Preparation of Sodium sulfide solution: add sodium sulfide to distilled/deionized water and bring volume to 10.0 mL. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C. Cool to 25˚C. Store anaerobically. L-Cystene Solution Composition per 10.0 m:" L-cysteine hydrochloride monohydrate, 1.5g Preparation of L-Cysteine solution: Add L-cysteine-HCL-H2O to distilled/deionized water and bring volume to 10.0 mL. Mix thoroughly. Sparge with dinitrogen. Autoclave for 15 min at 15 psi pressure-121˚C> Preparation of Medium: prepare and dispense medium under an oxygen-free 80% dihydrogen 20% carbon dioxide gas mixture. Add components, except vitamin solution, L-cystene solution, and sodium sulfide solution to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Sparge with H2/CO2. Autocalve for 15 min at 15 psi pressure-121˚C. Cool to 25˚C while sparging with H2/CO2. Aseptically and anaerobically add 10.0 mL vitamin solution, 10.0 mL of sterile L-cystene soltuion, and 10.0 mL of sterile sodium sulfide solution. Mix thoroughly. Aseptically and anaerobially distribute into sterile tubes or flasks. Alternately the medium can be distributed to tubes under anaerobic conditions and autoclaved in tubes prior to addition of substrate solution, vitamin solution, and sodium sulfide solution. Appropriate ammounts of these solutions can then be.....
A vitamin solution comprised of pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, calcium pantothenate, biotin, folic acid, p-aminobenzoic acid(4-aminobenzoic acid), and vitamin B12 (cobalamin). Prepared under an atmosphere of dihydrogen and carbon dioxide.
trace elements solution for DSMZ Medium 131
A trace elements solution comprised of magnesium sulfate, sodium chloride, disodium EDTA, manganese sulfate, zinc sulfate, cobalt chloride, calcium chloride, ferrous sulfate, copper sulfate, nickel chloride, potassium aluminum sulfate, sodium molybdate, and boric acid. Prepared under an atmosphere of dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104.pdf 104. PYG MEDIUM (modified) Trypticase peptone 5.00 g Peptone 5.00 g Yeast extract 10.00 g Beef extract 5.00 g Glucose 5.00 g K2HPO4 2.00 g Tween 80 1.00 ml Cysteine-HCl x H2O 0.50 g Resazurin 1.00 mg Salt solution (see below) 40.00 ml Distilled water 950.00 ml Haemin solution (see below) 10.00 ml Vitamin K1 solution (see below) 0.20 ml The vitamin K1, haemin solution and the cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.2 using 8 N NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2009 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase peptone, peptone, yeast extract, beef extract, glucose, potassium phosphate, tween 80 (polysorbate 80), cysteine hydrochloride, resazurin, salt solution, hemin solution, and vitamin K solution. Prepared under a atmosphere of carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium470.pdf 470. PSEUDOMONAS HALOPHILA MEDIUM Solution A: NaCl 46.8 g MgSO4 x 7 H2O 39.4 g NH4Cl 1.0 g Glycerol 5.0 g Trace element sol. SL-10 (see medium 320) 1.0 ml Vitamin solution (see medium 131) 10.0 ml Distilled water 890.0 ml Solution B: KH2PO4 1.0 g Distilled water 100.0 ml Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling. Adjust pH to 7.0 with 6 M NaOH or HCl. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, ammonium chloride, glycerol, trace elements, vitamins, and potassium phosphate.
DSM strains: 12906 Tessaracoccus bendigoensis DSM 12906 12890 Tetrasphaera australiensis 13193 Tetrasphaera australiensis 13192 Tetrasphaera japonica T1-X7
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium851.pdf 851. GLUCOSE SULFIDE MEDIUM Glucose 0.15 g Yeast extract 1.00 g (NH4)2SO4 0.50 g CaCO3 0.10 g Ca(NO3)2 0.10 g KCl 0.05 g K2HPO4 0.05 g MgSO4 x 7 H2O 0.05 g Na2S x 9 H2O 0.20 g Vitamin solution (see medium 131) 10.00 ml Distilled water 990.00 ml Adjust pH to 7.3. © 2011 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of glucose, yeast extract, ammonium sulfate, calcium carbonate, calcium nitrate, potassium chloride, potassium phosphate, magnesium sulfate, sodium sulfide, and vitamins.
Similar to DSMZ Medium 11, except a vitamin solution is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium11a.pdf 11a. MODIFIED MEDIUM 11 Prepare medium 11 with additional vitamin solution (see medium 131). © 2007 DSMZ GmbH - All rights reserved
A minerals salts, liquid culture medium comprised of artificial sea water, vitamins, sodium nitrate, sodium bicarbonate, and elemental sulfur. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1131.pdf 1131. HYDROGENIVIRGA OKINAWENSIS MEDIUM Modified MJ synthetic sea water (see below) 1000.0 ml Trace vitamins (see next page) 10.0 ml NaNO3 1.0 g NaHCO3 1.0 g Sulfur (powder) 3.0 g Mix ingredients except NaHCO3 and vitamin solution, adjust pH to 7.0 with NaOH, and autoclave under a N2 atmosphere. Filter-sterilize 8% NaHCO3 solution and add to the medium. Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) containing sulfur under a stream of a H2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Steam medium for 3 hr on each of 3 successive days. Prior to inoculation, add trace vitamins (sterile filtrated and stocked under a N2 atmosphere) to the medium. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v). Modified MJ synthetic sea water: NaCl 30.00 g K2HPO4 0.14 g CaCl2 x 2 H2O 0.50 g NH4Cl 0.50 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.18 g KCl 0.33 g FeSO4 x 7 H2O 0.01 g NiCl2 x 6 H2O 1.00 mg Na2SeO3 x 5 H2O 1.00 mg Trace element solution (see below) 10.00 ml Distilled water 1000.00 ml Trace element solution: Nitrilotriacetic acid 1.50 g MgSO4 x 7 H2O 3.00 g MnSO4 x X H2O 0.50 g NaCl 1.00 g FeSO4 x 7 H2O 0.10 g CoSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g ZnSO4 x 7 H2O 0.10 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g Distilled water 1000.00 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). Vitamin solution: Biotin 2.00 mg Folic acid 2.00 mg Pyridoxine-HCl 10.00 mg Thiamine-HCl x 2 H2O 5.00 mg Riboflavin 5.00 mg Nicotinic acid 5.00 mg D-Ca-pantothenate 5.00 mg Vitamin B12 0.10 mg p-Aminobenzoic acid 5.00 mg Lipoic acid 5.00 mg Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved
An artificial seawater comprised of sodium chloride, potassium phosphate, calcium chloride, ammonium chloride, magnesium suflate, magnesium chloride, potassium chloride, ferrous sulfate, nickel chloride, sodium selenite, and trace elements.
trace elements solution for DSMZ Medium 1131
A trace elements solution comprised of nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, and sodium molybdate.
A vitamin solution comprised of biotin, folic acid, pyridoxine hydrochloride, thiamine hydrochloride, riboflavin, nicotinic acid, calcium pantothenate, vitamin B12 (cobalamin), p-aminobenzoic acid (4-aminobenzoic acid), and lipoic acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium111.pdf 111. BEIJERINCKIA MEDIUM Glucose 10.0 g K2HPO4 0.8 g KH2PO4 0.2 g MgSO4 x 7 H2O 0.1 g FeSO4 x 7 H2O 20.0 mg MnSO4 x 6 H2O 2.0 mg ZnSO4 x 6 H2O 5.0 mg CuSO4 x 6 H2O 4.0 mg Na2MoO4 x 2 H2O 5.0 mg Agar 15.0 g Distilled water 950.0 ml Adjust pH to 6.5. Sterilize glucose separately (10 g in 50 ml H2O) and mix after cooling. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of glucose, potassium phosphate, magnesium sulfate, ferrous sulfate, manganese sulfate, zinc sulfate, copper sulfate, sodium molybdate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110a.pdf 110a. CMC MEDIUM (N2/CO2) Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg D-Glucose 4.0 g Cellobiose 1.0 g Maltose 1.0 g Starch (soluble) 1.0 g To make medium anoxic boil it, cool under 80% N2 + 20% CO2 gas atmosphere, add 0.5 g/l L-cysteine-HCl x H2O and dispense under same gas atmosphere into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 20 min. After autoclaving adjust pH of medium to pH 7 using a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 + 20% CO2 gas atmosphere. © 2014 DSMZ GmbH - All rights reserved
An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, D-glucose, cellobiose, maltose, soluble starch, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium110.pdf 110. CHOPPED MEAT MEDIUM WITH CARBOHYDRATES Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Glucose 4.0 g Cellobiose 1.0 g Maltose 1.0 g Starch (soluble) 1.0 g To make medium anoxic boil it, cool under nitrogen atmosphere, add 0.5 g/l cysteine hydrochloride and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.00 ml Vitamin K1 or Vitamin K3 solution (see below) 0.20 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. © 2010 DSMZ GmbH - All rights reserved
An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, glucose, cellobiose, maltose, soluble starch, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen.
DSM strains: 19147 Alistipes onderdonkii WAL 8169 = DSM 19147 19121 Alistipes shahii WAL 8301 2951 Anaerococcus tetradius ATCC 35098 2635 Asaccharospora irregularis DSM 2635 15896 Bacteroides acidifaciens JCM 10556 19024 Bacteroides caccae ATCC 43185 14838 Bacteroides cellulosilyticus DSM 14838 22519 Bacteroides clarus YIT 12056 20705 [Bacteroides] coagulans 26883 Bacteroides faecichinchillae JCM 17102 24798 Bacteroides faecis MAJ27 17565 Bacteroides finegoldii DSM 17565 22534 Bacteroides fluxus YIT 12057 1396 Bacteroides fragilis 2151 Bacteroides fragilis NCTC 9343 22535 Bacteroides oleiciplenus YIT 12058 1896 Bacteroides ovatus ATCC 8483 21004 Bacteroides paurosaccharolyticus JCM 15092 19346 Bacteroides propionicifaciens 19291 Bacteroides propionicifaciens DSM 19291 = JCM 14649 19673 Bacteroides pyogenes DSM 20611 = JCM 6294 20611 Bacteroides pyogenes 26882 Bacteroides rodentium JCM 16496 18765 Bacteroides salyersiae WAL 10018 = DSM 18765 = JCM 12988 19555 Bacteroides stercoris ATCC 43183 2079 Bacteroides thetaiotaomicron VPI-5482 2255 Bacteroides thetaiotaomicron 6597 Bacteroides uniformis ATCC 8492 1447 Bacteroides vulgatus ATCC 8482 3289 Bacteroides vulgatus 18836 Bacteroides xylanisolvens XB1A 21032 Barnesiella intestinihominis YIT 11860 18177 Barnesiella viscericola DSM 18177 2950 Blautia producta ATCC 27340 = DSM 2950 3507 Blautia producta 19528 Campylobacter gracilis 21671 Campylobacter hominis 21682 Campylobacter mucosalis 19458 Campylobacter showae 2779 Centipeda periodontii 23941 Cetobacterium somerae ATCC BAA-474 630 [Clostridium] bifermentans 631 [Clostridium] bifermentans 666 Clostridium cochlearium 1285 Clostridium cochlearium 2153 Clostridium cochlearium 6011 [Clostridium] colinum 27543 Peptoclostridium difficile 630 27544 Peptoclostridium difficile 28645 Peptoclostridium difficile 630 12750 Clostridium drakei 2631 Clostridium fallax DSM 2631 5565 Clostridium haemolyticum 627 Hathewaya histolytica 2158 Hathewaya histolytica 1286 [Clostridium] innocuum 5566 Clostridium novyi B str. ATCC 27606 2630 Clostridium paraputrificum 10364 Clostridium pascui 10365 Clostridium pascui 10366 Clostridium pascui 10367 Clostridium pascui 628 Clostridium perfringens 2943 Clostridium perfringens 11778 Clostridium perfringens 11779 Clostridium perfringens 11780 Clostridium perfringens 11781 Clostridium perfringens 11782 Clostridium perfringens 11783 Clostridium perfringens 11784 Clostridium perfringens 11785 Clostridium perfringens 11786 Clostridium perfringens 2632 Clostridium sardiniense 2141 [Clostridium] sordellii ATCC 9714 1985 Clostridium 4029 Clostridium 633 Clostridium sporogenes 634 Clostridium sporogenes 767 Clostridium sporogenes 1446 Clostridium sporogenes 1664 Clostridium sporogenes 1294 [Clostridium] sporosphaeroides DSM 1294 2636 Clostridium subterminale 6970 Clostridium subterminale 2485 Clostridium tertium 11744 Clostridium tetani 11745 Clostridium tetani 3979 Collinsella aerofaciens ATCC 25986 15921 Coprobacillus cateniformis JCM 10604 26242 Coprobacter fastidiosus NSB1 15641 Cryptobacterium curtum DSM 15641 20708 Dichelobacter nodosus A198 23057 Dichelobacter nodosus 3992 Dorea formicigenerans ATCC 27755 27370 Dysgonomonas macrotermitis 16107 Eggerthella sinensis 25476 Enorma massiliensis phI 3989 Holdemanella biformis DSM 3989 3983 Faecalitalea cylindroides ATCC 27803 3991 [Eubacterium] dolichum DSM 3991 2593 Eubacterium limosum 2594 Eubacterium limosum 18759 Eubacterium minutum ATCC 700079 27210 Falsiporphyromonas endometrii 1645 Filifactor villosus 6740 Flavonifractor plautii DSM 6740 19678 Fusobacterium necrophorum subsp. funduliforme ATCC 51357 19507 Fusobacterium nucleatum subsp. vincentii ATCC 49256 19508 Fusobacterium nucleatum subsp. fusiforme ATCC 51190 15657 Guggenheimella bovis 15480 Hespellia stercorisuis DSM 15480 22815 Jonquetella anthropi DSM 22815 22070 Leptotrichia trevisanii DSM 22070 1672 Megamonas hypermegale DSM 1672 19944 Megamonas rupellensis DSM 19944 3998 Mogibacterium timidum DSM 3998 23919 Murdochiella asaccharolytica 21255 Negativicoccus succinicivorans 24967 Parabacteroides chartae 29073 Parabacteroides chinchillae 19448 Parabacteroides goldsteinii DSM 19448 = WAL 12034 23371 Parabacteroides gordonii DSM 23371 16106 Paraeggerthella hongkongensis JCM 14552 19731 Paraprevotella clara YIT 11840 19681 Paraprevotella xylaniphila YIT 11841 3032 Peptoniphilus asaccharolyticus 20463 Peptoniphilus asaccharolyticus DSM 20463 2949 Peptostreptococcus anaerobius VPI 4330 = DSM 2949 23041 Peptostreptococcus russellii 20707 Porphyromonas asaccharolytica DSM 20707 23058 Porphyromonas bennonis DSM 23058 = JCM 16335 15663 Porphyromonas gulae DSM 15663 23370 Porphyromonas levii DSM 23370 23386 Porphyromonas somerae DSM 23386 23387 Porphyromonas uenonis DSM 23387 = JCM 13868 23917 Bacteroides heparinolyticus ATCC 35895 13386 Prevotella nigrescens ATCC 33563 1897 Propionibacterium acnes DSM 1897 15818 Propionibacterium australiense 23940 Pseudoflavonifractor capillosus ATCC 29799 21147 Pyramidobacter piscolens W5455 23594 Selenomonas bovis DSM 23594 19578 Selenomonas noxia ATCC 43541 2479 Selenomonas sp. ATCC 33150 5675 Tissierella praeacuta
An organic-rich liquic culture medium comprised of lean ground beef hydrolysate. To this, casitone, yeast extract, potassium phosphate, resazurin, cysteine, and cysteine hydrochloride are added. Prepared under an atmosphere of dinitrogen.
Similar to DSMZ Medium 78, except that an amino acid solution and vitamin solution are added.
DSM strains: 12168 Treponema brennaborense DSM 12168
An organic-rich, solid culture medium comprised of peptone, beef extract, glycerol, soil extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium80.pdf 80. GLYCEROL-SOIL MEDIUM Peptone 5.0 g Beef extract 3.0 g Glycerol 20.0 g Soil extract 150.0 ml Distilled water 850.0 ml Agar 15.0 g Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400 g with 960 ml of distilled water at 121˚C for one hour. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclaved in 200 ml quantities, and stored at room temperature until cleared by sedimentation. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 43067 Actinomadura madurae NBRC 14623 43236 Actinomadura madurae 2354 Gordonia alkanivorans 43247 Gordonia bronchialis DSM 43247 43249 Gordonia terrae 43342 Gordonia terrae 43231 Mycobacterium conceptionense 43212 Mycobacterium gordonae 43213 Mycobacterium gordonae 43222 Mycobacterium parascrofulaceum 43239 Mycobacterium phlei DSM 43239 = CCUG 21000 535 Pseudonocardia autotrophica 43103 Pseudonocardia autotrophica 658 Pseudonocardia petroleophila 43193 Pseudonocardia petroleophila
DSM strains: 621 Leucothrix mucor 5127 Leucothrix mucor DSM 2157 41682 Streptomyces hiroshimensis
An organic-rich, liquid culture medium containing tryptone and synthetic seawater.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium82.pdf 82. BHI-GLUCOSE MEDIUM Brain heart infusion 18.5 g Glucose 5.0 g Agar 12.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
DSM strains: 43117 Actinomadura pelletieri 43118 Actinomadura pelletieri 43031 Actinoplanes auranticolor 43046 Actinoplanes missouriensis 43019 Actinoplanes philippinensis 43045 Agromyces ramosus 20651 Corynebacterium minutissimum 43037 Dermatophilus congolensis 43043 Intrasporangium calvum DSM 43043 43218 Mycobacterium diernhoferi 43058 Mycobacterium 43024 Nocardia brevicatena NBRC 12119 43005 Nocardia cyriacigeorgica 43003 Nocardia farcinica 43010 Nocardia otitidiscaviarum 43242 Nocardia otitidiscaviarum 43069 Nocardia 43027 Pseudonocardia thermophila 43188 Rhodococcus erythropolis 43113 Saccharopolyspora rectivirgula 43114 Saccharopolyspora rectivirgula 2072 Streptococcus pyogenes 2073 Streptococcus pyogenes 2074 Streptococcus pyogenes
An organic-rich, solid culture medium comprised of brain heart infusion, glucose, and agar.
A minerals-salts, solid culture medium that contains potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanadate, trace elements, agar, ferric ammonium citrate and sodium bicarbonate. Prepared under an atmosphere of dioxygen, carbon dioxide, dihydrogen, and dinitrogen.
A minerals salts, liquid culture medium containing potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanate, ferric ammonium citrate, sodium carbonate and trace elements. Prepared under an atmosphere of dioxygen, carbon dioxide, hydrogen, and dinitrogen.
mineral medium for chemolithotrophic growth (H-3)
DSM strains: 653 Achromobacter ruhlandii 550 Acidovorax facilis 620 Acidovorax facilis 649 Acidovorax facilis 3314 Acidovorax 2625 Alcaligenes 5537 Alcaligenes 334 Ancylobacter aquaticus 2454 Ancylobacter aquaticus 2456 Ancylobacter aquaticus 1108 Ancylobacter polymorphus 2457 Ancylobacter polymorphus 2667 Ancylobacter polymorphus 3910 Ancylobacter polymorphus 3911 Ancylobacter polymorphus 3912 Ancylobacter polymorphus 3913 Ancylobacter polymorphus 3914 Ancylobacter polymorphus 1106 Ancylobacter 1107 Ancylobacter 2668 Ancylobacter 2669 Ancylobacter 1277 Ancylobacter vacuolatus 1124 Azohydromonas australica DSM 1124 1122 Azohydromonas lata 1123 Azohydromonas lata 1721 Azohydromonas 1722 Azohydromonas 1723 Azohydromonas 2787 Nitrospirillum amazonense 2788 Nitrospirillum amazonense Y2 2789 Nitrospirillum amazonense 1838 Azospirillum lipoferum 1840 Azospirillum lipoferum 1841 Azospirillum lipoferum 1860 Azospirillum lipoferum 2291 Azospirillum lipoferum 2292 Azospirillum lipoferum 2293 Azospirillum lipoferum 2294 Azospirillum lipoferum 1726 Azospirillum sp. A1-3 1727 Azospirillum 1842 Azospirillum 16346 Beijerinckia 1724 Beijerinckia 1755 Bradyrhizobium japonicum 1756 Bradyrhizobium japonicum 1982 Bradyrhizobium japonicum 1749 Burkholderia fungorum 11915 Chrysiogenes arsenatis DSM 11915 2839 Cupriavidus metallidurans CH34 529 Cupriavidus necator 530 Cupriavidus necator 531 Cupriavidus necator 538 Ralstonia eutropha H16 539 Cupriavidus necator 540 Cupriavidus necator 541 Cupriavidus necator 428 Cupriavidus necator 430 Cupriavidus necator 515 Cupriavidus necator 516 Cupriavidus necator 517 Cupriavidus necator 518 Cupriavidus necator 542 Cupriavidus necator 543 Cupriavidus necator 544 Cupriavidus necator 545 Cupriavidus necator 546 Cupriavidus necator 547 Cupriavidus necator 551 Cupriavidus necator 30029 Cupriavidus necator 723 Derxia gummosa DSM 723 1846 Derxia gummosa 732 Herbaspirillum autotrophicum 733 Herbaspirillum autotrophicum 2913 Hydrogenobacter hydrogenophilus 15341 Hydrogenophaga defluvii 619 Hydrogenophaga flava 650 Hydrogenophaga palleronii 63 Hydrogenophaga palleronii 1034 Hydrogenophaga pseudoflava 2082 Hydrogenophaga taeniospiralis 21442 Hydrogenophilus islandicus 413 Paracoccus denitrificans 415 Paracoccus denitrificans 1405 Paracoccus denitrificans 1403 Paracoccus pantotrophus 1404 Paracoccus pantotrophus 2666 Starkeya 21436 Thiomonas islandica 645 Variovorax paradoxus 646 Variovorax paradoxus 647 Variovorax paradoxus 1070 Variovorax paradoxus 1071 Variovorax paradoxus 1072 Variovorax paradoxus 30034 Variovorax paradoxus 3770 Xanthobacter agilis 431 Xanthobacter autotrophicus 432 Xanthobacter autotrophicus 597 Xanthobacter autotrophicus 685 Xanthobacter autotrophicus 1393 Xanthobacter autotrophicus
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium83.pdf 83. CZAPEK PEPTONE AGAR Sucrose 30.00 g NaNO3 3.00 g K2HPO4 1.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g FeSO4 x 7 H2O 0.01 g Yeast extract 2.00 g Peptone 5.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.3. © 2007 DSMZ GmbH - All rights reserved
Is an organic rich, solid culture medium comprised of sucrose, sodium nitrate, potassium phosphate, magnesium sulfate, potassium chloride, iron sulfate, yeast extract, peptone, and agar.
DSM strains: 43148 Actinoplanes campanulatus 43149 Actinoplanes digitatis 43146 Actinoplanes italicus 43150 Actinoplanes lobatus 43151 Actinoplanes regularis 43147 Actinoplanes utahensis 44348 Amycolatopsis eurytherma 44349 Amycolatopsis eurytherma 44467 Amycolatopsis sacchari 44468 Amycolatopsis sacchari 44469 Amycolatopsis sacchari 44470 Amycolatopsis sacchari 44592 Amycolatopsis vancoresmycina DSM 44592 44439 Gordonia polyisoprenivorans 43062 Laceyella sacchari 44438 Micromonospora aurantiaca 43127 Micromonospora chalcea 43036 Micromonospora echinospora 43171 Micromonospora halophytica 44983 Micromonospora rifamycinica 43312 Micromonospora 43313 Micromonospora 43314 Micromonospora 43024 Nocardia brevicatena NBRC 12119 44538 Nocardia veterana 44412 Nocardiopsis alba 44516 Nocardiopsis alba 44552 Nocardiopsis alba 44550 Nocardiopsis composta 44551 Nocardiopsis composta 44497 Nocardiopsis sp. 64/93 44407 Nocardiopsis exhalans 44659 Nocardiopsis sp. DSM 44659 44450 Nocardiopsis 43039 Pilimelia anulata 43040 Pilimelia terevasa 44945 Planifilum fulgidum 43177 Planomonospora parontospora subsp. parontospora 43110 Promicromonospora citrea 44525 Pseudonocardia kongjuensis 43195 Pseudonocardia saturnea 43169 Pseudonocardia 44631 Saccharomonospora azurea NA-128 43068 Saccharomonospora azurea 43017 Saccharomonospora viridis DSM 43017 43115 Saccharomonospora viridis
An organic-rich, solid culture medium comprised of rolled oats, agar, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf 84. ROLLED OATS MINERAL MEDIUM Rolled oats 20.0 g Agar 20.0 g Trace element solution (see below) 1.0 ml Distilled water 1000.0 ml Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml pH 7,2 © 2007 DSMZ GmbH - All rights reserved
DSM strains: 44705 Acrocarpospora macrocephala 44706 Acrocarpospora pleiomorpha 44982 bacterium 12202 43889 Actinoalloteichus cyanogriseus DSM 43889 45178 Actinocatenispora rupis 44816 Actinocatenispora thailandica 44254 Actinocorallia herbida 44770 Actinomadura glauciflava DSM 44770 46198 Actinomadura latina 46201 Actinomadura latina 43380 Actinomadura madurae 43381 Actinomadura madurae 44485 Actinomadura mexicana 44197 Actinomadura namibiensis 44761 Actinomadura nitritigenes 44762 Actinomadura nitritigenes 43750 Actinomadura rubrobrunea 43751 Actinomadura rubrobrunea 44003 Actinomadura 44763 Actinomadura 43765 Actinomadura vinacea 44433 Actinomadura viridilutea 46643 Actinoplanes globisporus 44926 Actinospica acidiphila 44927 Actinospica robiniae DSM 44927 44899 Catellatospora bangladeshensis 44900 Catellatospora chokoriensis 44901 Catellatospora coxensis 44128 Catenuloplanes crispus 44578 Clavisporangium rectum 44171 Glycomyces tenuis DSM 44171 44071 Herbidospora cretacea 44127 Herbidospora cretacea 44786 Kitasatospora gansuensis 44790 Kitasatospora kifunensis 44788 Kitasatospora paranensis 44943 Kitasatospora recifensis 44789 Kitasatospora terrestris 44826 Kitasatospora viridis 43870 Kutzneria albida DSM 43870 43851 Kutzneria kofuensis 43850 Kutzneria viridogrisea 43355 Laceyella putida 43353 Laceyella sacchari 43354 Laceyella sacchari 43356 Laceyella sacchari 45262 Laceyella tengchongensis 43885 Lechevalieria flava 15081 Lechevalieria xinjiangensis 44784 Longispora albida DSM 44784 44681 Microbispora corallina 44105 Microbispora griseoalba 43166 Microbispora rosea subsp. aerata 43176 Microbispora rosea subsp. aerata 43840 Microbispora rosea subsp. aerata 43025 Microbispora rosea subsp. rosea 43165 Microbispora rosea subsp. rosea 43837 Microbispora rosea subsp. rosea 43838 Microbispora rosea subsp. rosea 43839 Microbispora rosea subsp. rosea 43143 Micromonospora coerulea 43819 Micromonospora inositola 44579 Microtetraspora malaysiensis 44487 Nonomuraea asiatica 45128 Nonomuraea bangladeshensis 43767 Nonomuraea roseola 44170 Nonomuraea roseoviolacea subsp. carminata 43041 Planobispora longispora 43051 Planobispora rosea 43178 Planomonospora venezuelensis 44746 Planotetraspora silvatica 44698 Pseudonocardia chloroethenivorans 44072 Rhodococcus opacus 43401 Saccharopolyspora hirsuta 44936 Sphaerisporangium rubeum 44325 Spirilliplanes yamanashiensis 41754 Streptacidiphilus carbonis 44847 Streptacidiphilus griseisporus 44824 Streptacidiphilus luteoalbus 42024 Streptomyces abyssalis 41644 Streptomyces aculeolatus 41781 Streptomyces bungoensis 41447 Streptomyces chartreusis NRRL 3882 41827 Streptomyces costaricanus 41984 Streptomyces endophyticus 42021 Streptomyces glycovorans 40661 Streptomyces griseus 41944 Streptomyces guanduensis 43030 Streptomyces humiferus 42104 Streptomyces klenkii 41697 Streptomyces malaysiensis 42004 Streptomyces mordarskii 41804 Streptomyces reticuliscabiei 42105 Streptomyces smyrnaeus 40689 Streptomyces 40886 Streptomyces 40887 Streptomyces 40888 Streptomyces 40889 Streptomyces 40979 Streptomyces 41373 Streptomyces 41374 Streptomyces 41375 Streptomyces 41376 Streptomyces 41377 Streptomyces 41378 Streptomyces 41379 Streptomyces 41380 Streptomyces 41381 Streptomyces 41382 Streptomyces 41383 Streptomyces 41384 Streptomyces 41356 Streptomyces 41357 Streptomyces 41358 Streptomyces 41360 Streptomyces 41361 Streptomyces 41362 Streptomyces 41363 Streptomyces 41364 Streptomyces 41365 Streptomyces 41366 Streptomyces 41367 Streptomyces 41368 Streptomyces 41369 Streptomyces 41370 Streptomyces 41371 Streptomyces 41372 Streptomyces 41298 Streptomyces 41319 Streptomyces 41327 Streptomyces 41349 Streptomyces 41350 Streptomyces 41351 Streptomyces 41352 Streptomyces 41353 Streptomyces 41385 Streptomyces 41386 Streptomyces 41387 Streptomyces 41388 Streptomyces 41389 Streptomyces 41616 Streptomyces 41617 Streptomyces 41618 Streptomyces 41619 Streptomyces 41694 Streptomyces 41924 Streptomyces specialis 42057 Streptomyces staurosporininus 41679 Streptomyces sulfonofaciens 42019 Streptomyces sundarbansensis 44293 Streptomyces thermocarboxydus 41700 Streptomyces thermocoprophilus 41740 Streptomyces thermodiastaticus 42015 Streptomyces xinghaiensis S187 42022 Streptomyces xishensis 41945 Streptomyces yanglinensis 43023 Streptosporangium album 44109 Streptosporangium brasiliense 45034 Streptosporangium canum 45033 Streptosporangium sp. 7108 43948 Streptosporangium nondiastaticum 43021 Streptosporangium roseum DSM 43021 44095 Streptosporangium rubrum 43137 Streptosporangium 43138 Streptosporangium 43394 Streptosporangium 44662 Streptosporangium subroseum 43849 Streptosporangium violaceochromogenes 43872 Streptosporangium violaceochromogenes 44112 Streptosporangium vulgare 44663 Streptosporangium yunnanense 43184 Thermoactinomyces vulgaris 43372 Thermoactinomyces vulgaris 43038 Thermobispora bispora 43833 Thermobispora bispora DSM 43833 43186 Thermopolyspora flexuosa 44794 Virgisporangium aurantiacum 44793 Virgisporangium ochraceum
An organic-rich, liquid culture medium comprised of nitrilotriacetic acid, calcium sulfate, magnesium sulfate, sodium chloride, potassium nitrate, sodium nitrate, sodium phosphate, ferric chloride, manganese sulfate, zinc sulfate, boric acid, copper sulfate, sodium molybdate, cobalt chloride, tryptone, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium86.pdf 86. CASTENHOLZ MEDIUM Nitrilotriacetic acid (Titriplex I) 100.00 mg CaSO4 x 2 H2O 60.00 mg MgSO4 x 7 H2O 100.00 mg NaCl 8.00 mg KNO3 103.00 mg NaNO3 689.00 mg Na2HPO4 x 2 H2O 140.00 mg FeCl3 x 6 H2O 0.47 mg MnSO4 x H2O 2.20 mg ZnSO4 x 7 H2O 0.50 mg H3BO3 0.50 mg CuSO4 x 5 H2O 25.00 μg Na2MoO4 x 2 H2O 25.00 μg CoCl2 x 6 H2O 46.00 μg Tryptone 1.00 g Yeast extract 1.00 g Distilled water 1000.00 ml Adjust pH to 8.2 with NaOH. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 11376 Meiothermus cerbereus DSM 11376 11377 Meiothermus cerbereus 9957 Meiothermus chliarophilus DSM 9957 9946 Meiothermus silvanus DSM 9946 14542 Meiothermus taiwanensis DSM 14542 14543 Meiothermus taiwanensis 19627 Microcella putealis 14833 Tepidimonas aquatica 14834 Thermomonas hydrothermalis 5718 Thermonema lapsum 23/9 5719 Thermonema lapsum 625 Thermus aquaticus 12092 Thermus oshimai DSM 12092 12093 Thermus scotoductus
An organic rich, solid culture medium comprised of yeast extract, peptone, glucose, calcium carbonate, and agar. Given the pKa of calcium carbonate (9), the pH of the solution should be about 9.0.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium85.pdf 85. GLUCOSE PEPTONE MEDIUM Yeast extract 10.0 g Bacto peptone (Difco) 20.0 g Glucose 10.0 g CaCO3 10.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of yeast extract, glycylglycine, sodium phosphate, magnesium sulfate, potassium nitrate, sodium nitrate, sodium chloride, calcium chloride, ferric citrate, and trace elements. Prepared under an atmosphere of dinitrogen.
DSM strains: 13941 Roseiflexus castenholzii DSM 13941 9485 Chloroflexus aggregans DSM 9485 9486 Chloroflexus aggregans 635 Chloroflexus aurantiacus J-10-fl 636 Chloroflexus aurantiacus 637 Chloroflexus aurantiacus 638 Chloroflexus aurantiacus
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium87.pdf 87. CHLOROFLEXUS MEDIUM (modified) Yeast extract 1.00 g Glycyl-glycine 1.00 g Na2HPO4 x 2 H2O 0.10 g MgSO4 x 7 H2O 0.10 g KNO3 0.10 g NaNO3 0.50 g NaCl 0.10 g CaCl2 x 2 H2O 0.05 g Fe(III) citrate solution (0.1 g in 100 ml H2O) 5.00 ml Trace element solution SL-6 (see medium 27) 1.00 ml Distilled water 1050.00 ml Adjust pH to 8.2. Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121˚C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.015% end concentration, see medium 27) to each bottle. This medium can be stored for several months. Incubate the culture at 50˚C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366). © 2007 DSMZ GmbH - All rights reserved
DSM strains: 14542 Meiothermus taiwanensis DSM 14542 14543 Meiothermus taiwanensis
Similar to DSMZ Medium 86, except tryptone is omitted and peptone and monosodium glutamate are added. The pH is decreased to 7.8 with sodium hydroxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium86a.pdf 86a. MODIFIED CASTENHOLZ MEDIUM Use medium 86, but replace the tryptone with 3 g/l peptone and add 1 g/l mono sodium glutamate. The final pH should be adjusted to 7.8 with NaOH. Solid media is prepared by the addition of 2 - 2.5% agar. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 87, except a vitamin solution is added and the pH is decreased to 7.5.
DSM strains: 9485 Chloroflexus aggregans DSM 9485 9486 Chloroflexus aggregans 13941 Roseiflexus castenholz DSM 13941
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88.pdf 88. SULFOLOBUS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Sterilize substrates separately and add to the medium after autoclaving. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348 omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and ore by heating at 150°C over night. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, and DSM 12421 adjust pH of medium to 3.0 – 3.5. For DSM 6482 and DSM 10039 reduce amount of yeast extract to 0.20 g/l and supplement medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. For DSM 9789 and DSM 9790 use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993 supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18786 use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150 °C over night. Adjust pH of the medium to 0.8. For DSM 29038 supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099 use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 – 3.0. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 88, except the concentration of yeast extract is reduced, and D-glucose and elemental sulfur are added. The pH is increased to 2.5-3.0.
DSM 29038 is Acidianus sp. ALE 1 = Candidatus Acidianus copahuensis
Similar to DSMZ Medium 88, except potassium tetrathionate is added. The pH is increased to 2.5.
Similar to DSMZ Medium 88, except the concentration of yeast extract is decreased and a sulfide mineral is added. The pH is decreased to 0.8.
DSM 18786 is Acidianus sulfidivorans JP7
Similar to DSMZ Medium 88, except D-glucose and casamino acids are added. The pH is increased to 3.0.
DSM 9789 is Picrophilus oshimae DSM 9789 DSM 9790 is Picrophilus torridus DSM 9790
Similar to DSMZ Medium 88, except the concentration of yeast extract is increased and the pH is decreased to 1.0.
DSMZ Medium 88.4 -< for DSM 9789 and DSM 9790
DSMZ Medium 88.3 -< for DSM 6482 and DSM 10039
DSM 6482 is Sulfolobus metallicus DSM 6482 = JCM 9184 DSM 10039 is Metallosphaera prunae
Similar to DSMZ Medium 88, except the amount of yeast extract is reduced and elemental sulfur is added.
DSMZ Medium 88.2 -< for DSM 5389, DSM 7519, and DSM 12421
Similar to DSMZ Medium 88, except the pH is increased to 3.0-3.5.
DSM 5389 is Sulfolobus shibatae B12 DSM 7519 is Metallosphaera hakonensis JCM 8857 DSM 12421 is Sulfurisphaera ohwakuensis
DSM 5348 is Metallosphaera sedula DSM 5348
Similar to DSMZ Medium 88, except yeast extract is omitted and elemental sulfur and a sulfide mineral are added.
DSM strains: 29038 Candidatus Acidianus copahuensis 18786 Acidianus sulfidivorans JP7 7519 Metallosphaera hakonensis 10039 Metallosphaera prunae 639 Sulfolobus acidocaldarius DSM 639 6482 Sulfolobus metallicus DSM 6482 = JCM 9184 5389 Sulfolobus shibatae B12 16993 Sulfolobus tokodaii str. 7 12421 Sulfurisphaera ohwakuensis 18247 Thermobacillus composti KWC4 14429 Vulcanisaeta distributa DSM 14429 14430 Vulcanisaeta souniana
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric chloride, manganese chloride, sodium tetraborate, zinc sulfate, vanadyl sulfate, cobalt sulfate, and yeast extract.
An organic-rich, liquid culture medium comprised of elemental sulfur, yeast extract, soluble starch, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88b.pdf 88b. V24N MEDIUM Prepare medium 88 without yeast extract, then add (per litre): Sulfur, powdered 1.0 g Yeast extract 0.2 g Starch, soluble 2.0 g Resazurin 0.4 mg Na2S x 9 H2O 0.5 g Adjust the pH to 6.0 with H2SO4 (25% v/v). Prepare the medium under 100% N2 gas phase and distribute anaerobically into rubber stoppered tubes or bottles (presterilized by autoclaving). The medium is sterilized by heating 2 hours at 85 ˚C on each of two following days. © 2007 DSMZ GmbH - All rights reserved
DSM 2161 is Desulfurococcus mobilis DSM 2161 DSM 2162 is Desulfurococcus mucosus DSM 2162
Similar to DSMZ Medium 88a, except the concentration of yeast extract is increased and the pH is increased to 5.5.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium88a.pdf 88a. SULFOLOBUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 10.00 g Yeast extract (Difco) 0.50 g Na2S x 9 H2O 0.50 g Demineralized water 1000.00 ml Dissolve ingredients (except sulfur, yeast extract and sulfide), adjust pH of the salt solution at room temperature to 4.0 using 1 N H2SO4 and sparge medium with 100% N2 gas to make it anoxic. Dispense medium under same gas atmosphere in anoxic vials and autoclave. Steam sulfur for 3 hr on each of 3 successive days. Add sulfur aseptically to the autoclaved medium while retaining anoxic conditions. Add yeast extract and sulfide from sterile anoxic stock solutions prepared under N2 gas. Prior to inoculation check pH and adjust to 4.0, if necessary. For DSM 2161 and DSM 2162 use 1.00 g/l yeast extract and adjust pH of medium to 5.5. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 88a.1 -< for DSM 2161 and DSM 2162
Similar to DSMZ Medium 88, except elemental sulfur and sodium sulfide are added. Prepared under an atmosphere of dinitrogen and the pH is increased to 4.0.
An organic-rich, solid culture medium comprised of malt extract, soy peptone, and agar.
DSM strains: none found in BacDive database
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium90.pdf 90. MALT EXTRACT PEPTONE AGAR Malt extract 30.0 g Soya peptone 3.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 5.6. Sterilize at 121˚C for 10 min © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of casein peptone, tryptic digest (casitone), yeast extract, sodium lactate, and agar.
DSM strains: 20272 Propionibacterium acidipropionici 20273 Propionibacterium acidipropionici 20271 Propionibacterium freudenreichii subsp. freudenreichii 20270 Propionibacterium freudenreichii subsp. shermanii 20274 Propionibacterium jensenii 20275 Propionibacterium jensenii 20278 Propionibacterium jensenii 20279 Propionibacterium jensenii 20535 Propionibacterium jensenii DSM 20535 20276 Propionibacterium thoenii DSM 20276 20277 Propionibacterium thoenii
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium91.pdf 91. PROPIONIBACTERIUM AGAR Casein peptone, tryptic digest 10.0 g Yeast extract 5.0 g Na-lactate 10.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 15826 Luteococcus peritonei
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92a.pdf 92a. MODIFIED MEDIUM 92 Prepare medium 92. Add vitamin solution (see medium 131). © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 92, except that a vitamin solution is added.
An organic-rich, liquid culture medium comprised of tryptic soy broth, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium92.pdf 92. TRYPTICASE SOY YEAST EXTRACT MEDIUM Trypticase soy broth 30.0 g Yeast extract 3.0 g Agar, if necessary 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2012 DSMZ GmbH - All rights reserved
DSM strains: 684 Desulfuromonas acetoxidans DSM 684 1675 Desulfuromonas acetoxidans 1676 Desulfuromonas acetoxidans
A minerals-salts, liquid culture medium containing potassium phospahte, ammonium chloride, magnesium sulfate, magnesium chloride, sodium chloride, calcium chloride, sodium sulfate, trace elements, sodium malate, yeast extract, resazurin, ehtanol, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium95.pdf 95. DESULFUROMONAS ACETOXIDANS MEDIUM Solution A: KH2PO4 1.00 g NH4Cl 0.30 g MgSO4 x 7 H2O 1.00 g MgCl2 x 6 H2O 2.00 g NaCl 20.00 g CaCl2 x 2 H2O 0.10 g Na2SO4 1.77 g Trace element solution SL-4 (see medium 14) 10.00 ml Na2-DL-malate 2.66 g Yeast extract 0.50 g Resazurin 1.00 mg Distilled water 940.00 ml Adjust pH to 6.0 with 2 N NaOH. Solution B: Ethanol 0.30 ml Distilled water 2.70 ml Solution C: NaHCO3 1.85 g Distilled water 40.00 ml Solution D: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Bring solution A to the boil, then cool down to room temperature under 100% N2 gas atmosphere. Dispense medium under same gas atmosphere in culture vessels and sterilize by autoclaving. Solutions B and D are autoclaved separately under 100% N2 gas atmosphere. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. The medium is completed before use by adding appropriate amounts of solutions B, C and D to solution A. Final pH is adjusted to 7.2. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, solid microbiological culture medium comprised of peptone, meat extract, sodium chloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium115.pdf 115. VIBRIO NATRIEGENS MEDIUM To medium 1 add 1.5% NaCl. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium114.pdf 114. PARACOCCUS HALODENITRIFICANS MEDIUM To medium 1 add 6% NaCl. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 113, except sodium chloride is added such that the salinity is increased to marine salinity levels.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium113a.pdf 113a. SULFURIMONAS HONGKONGENSIS MEDIUM Solution A: NaCl 25.0 g KH2PO4 2.0 g KNO3 2.0 g NH4Cl 1.0 g MgSO4 x 7 H2O 0.8 g Trace element solution SL-4 (see medium 14) 2.0 ml Distilled water 930.0 ml Adjust pH to 7.0 with NaOH. Solution B: Na2S2O3 x 5 H2O 5.0 g Distilled water 40.0 ml Solution C: NaHCO3 1.0 g Distilled water 20.0 ml Solution D: FeSO4 x 7 H2O 2.0 mg H2SO4 (0.1 N) 1.0 ml Solution E: Vitamins solution (see medium 141) 10.0 ml Solutions A, B and D are prepared under 100% N2 gas atmosphere and sterilized by autoclaving at 121˚C for 15 min. Solution C is sterilized by filtration or by autoclaving in a tightly closed vessel under an atmosphere of 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. After sterilization combine the five solutions and distribute as required under 100% N2 gas atmosphere. For solid medium add 15 g agar to solution A. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium phosphate, potassium nitrate, ammonium chloride, magnesium sulfate, trace elements, sodium selenite, sodium bicarbonate, and ferrous sulfate. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium113.pdf 113. THIOBACILLUS DENITRIFICANS MEDIUM Solution A: KH2PO4 2.0 g KNO3 2.0 g NH4Cl 1.0 g MgSO4 x 7 H2O 0.8 g Trace element solution SL-4 (see medium 14) 2.0 ml Distilled water 940.0 ml Adjust pH to 7.0 with NaOH. Solution B: Na2S2O3 x 5 H2O 5.0 g Distilled water 40.0 ml Solution C: NaHCO3 1.0 g Distilled water 20.0 ml Solution D: FeSO4 x 7 H2O 2.0 mg H2SO4 (0.1 N) 1.0 ml Solutions A, B and D are sterilized separately by autoclaving at 121˚C for 15 min under nitrogen atmosphere. Solution C is sterilized by filtration or by autoclaving in a tightly closed vessel under an atmosphere of 80% N2 and 20% CO2 gas atmosphere. After sterilization combine the four solutions and distribute as required under nitrogen atmosphere. For solid medium add 15 g agar to solution A. © 2010 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 120a, except the concentration of sodium bicarbonate is increased, the concentration of methanol is decreased, and the pH is increased slightly to 6.8-7.0. Prepared under an atmosphere of dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120a.pdf 120a. METHANOSARCINA BARKERI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Resazurin 0.50 mg NaHCO3 0.85 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 gas and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.5 - 6.8. For DSM 4556 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 10334 and DSM 13486 increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 – 7.0 and add only 5.00 ml/l methanol. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1, except diaminopimelic acid (2,6-diaminopimelic acid) is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium118.pdf 118. DAP-NUTRIENT AGAR To medium 1 add 100 mg DL-diaminopimelic acid. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium119.pdf 119. METHANOBACTERIUM MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see below) 50.00 ml Fatty acid mixture (see below) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 930.00 ml Dissolve ingredients except bicarbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. Sludge fluid: Add 0.4% yeast extract to sludge from an anaerobic digester, and after gassing with nitrogen gas for a few minutes incubate it at 37˚C for 24 hours. Then centrifuge the sludge at 13000 g and autoclave the resulting, clear supernatant in screw-capped vessels under nitrogen gas. The sludge fluid can be stored at room temperature in the dark. Fatty acid mixture: Valeric acid 0.50 g Isovaleric acid 0.50 g 2-Methylbutyric acid 0.50 g Isobutyric acid 0.50 g Distilled water 20.00 ml Adjust pH to 7.5 with conc. NaOH. For DSM 1093 supplement medium after autoclaving with 0.50 g/l coenzyme M (mercaptoethanesulfonic acid) added from a filter-sterilized anoxic stock solution prepared under N2. For DSM 2030 adjust pH to 6.5 and add sterile 80% H2 and 20% CO2 gas to 2 bar overpressure after inoculation. For DSM 6216 increase amount of Na-acetate to 3.00 g/l. For DSM 7057 supplement medium with 2.00 g/l Na2SO4. For DSM 7256 omit sludge fluid and prepare medium with 20% (v/v) clarified rumen fluid (see medium 1310). For DSM 9575 add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure after inoculation. For DSM 15163 adjust pH of final medium to 6.0 For DSM 16632 and DSM 16643 replace sludge fluid with the same volume of clarified rumen fluid (see medium 1310), and supplement medium with 2.00 g/l Trypticase peptone and 10.00 ml/l of a vitamin solution (see medium 141). For DSM 25720 supplement medium after autoclaving with 1.00 ml/l selenite-tungstate solution (see medium 385) and 2.50 g/l methanol added from a sterile anoxic stock solution. Adjust pH of completed medium to 7.5. Add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25824 supplement medium after autoclaving with 0.10 g/l 2- mercaptoethanesulfonic acid (coenzyme M) added from an anoxic stock solution sterilized by filtration and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25939 adjust the final pH of the medium to 7.2 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. For DSM 25945 adjust the final pH of the medium to 7.4 and add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure after inoculation. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, calcium chloride, ferrous sulfate, trace elements, yeast extract, sodium acetate, sodium formate, sludge fluid, fatty acids, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, dinitrogen, and carbon dioxide.
Similar to DSMZ Medium 119, except coenzyme M is added.
DSM 1093 is Methanobrevibacter ruminantium M1
Similar to DSMZ Medium 119, except pH is reduced to 6.5 and the atmosphere is dihydrogen and carbon dioxide.
Similar to DSMZ Medium 119, except the concentration of sodium acetate is increased.
Similar to DSMZ Medium 119, except sodium sulfate is added.
Similar to DSMZ Medium 119, except sludge fluid is omitted and clarified rumen fluid is added.
Similar to DSMZ Medium 119, except the atmosphere is dihydrogen and carbon dioxide.
DSM 15163 is Methanobrevibacter acididurans
Similar to DSMZ Medium 119, except the pH is reduced to 6.0.
DSMZ Medium 119.8 -< for DSM 16632 and DSM 16643
DSM 16632 is Methanobrevibacter olleyae DSM 16643 is Methanobrevibacter millerae
Similar to DSMZ Medium 119, except sludge fluid is omiited, and trypicase peptone, vitamin solution, and clarified rumen fluid is added.
DSM 25720 is Methanomassiliicoccus luminyensis B10
Similar to DSMZ Medium 119, except selenite-tungstate solution and methanol are added. The pH is increased to 7.5 and the atmosphere is dihydrogen and carbon dioxide.
DSM 25824 is Methanobrevibacter boviskoreani JH1
Similar to DSMZ Medium 119, except coenzyme is added and the atmosphere is dihydrogen and carbon dioxide.
Similar to DSMZ Medium 119, except the pH is increased to 7.2 and the atmosphere is dihydrogen and carbon dioxide.
Similar to DSMZ Medium 119, except the pH is increased to 7.4 and the atmosphere is dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104b.pdf 104b. PY + X MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except glucose and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 1736 replace glucose with 5.0 g/l D-fructose. For DSM 6555 replace glucose with 5.0 g/l xylan. For DSM 12857, DSM 13479 and DSM 13480 omit glucose. For DSM 13181 reduce amount of glucose to 2.0 g/l. For DSM 14760, DSM 14761 and DSM 14762 omit glucose and replace it with 8.0 g/l Na2-succinate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 19022 replace glucose with 2.0 g/l Na-pyruvate. For DSM 21120 replace glucose with 5.0 g/l Na-L-glutamate and supplement medium after autoclaving with 1 ml/l of a sterile anoxic stock solution of the vitamins solution of medium 503. For DSM 21650 omit glucose and adjust pH to 8.5. For DSM 21761 replace glucose with 5.0 g/l D-fructose and adjust pH to 7.5 – 8.0. For DSM 24749 supplement medium with 10.0 g/l NaCl and replace glucose with 5.0 g/l D-fructose. Adjust pH to 7.3 – 7.5. For DSM 26752 adjust pH to 8.0 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 27512 adjust pH to 8.5 with a sterile anoxic stock solution of Na2CO3 (5% w/v). For DSM 28571 replace glucose with 1.0 g/l D-cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase peptone, pepsi-digested peptone from meat (meat peptone), yeast extract, salt solution, rezasurin, L-cysteine hydrochloride, and D-glucose. Prepared under an atmosphere of dinitrogen.
DSM 1736 is Dendrosporobacter quercicolus DSM 1736
Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose is added.
Similar to DSMZ Medium 104b, except glucose is omitted and xylan is added.
DSM 12857 is [Clostridium] amygdalinum DSM 13479 is Hungatella hathewayi DSM 13479 DSM 13480 is Hungatella hathewayi
DSMZ Medium 104b.3 -< for DSM 12857, DSM 13479 and DSM 13480
Similar to DSMZ Medium 104b, except glucose is omitted.
DSM 13181 is Anaerobaculum mobile DSM 13181
Similar to DSMZ Medium 104b, except the concentration of glucose is reduced.
DSM 14760 is Phascolarctobacterium faecium DSM 14760 DSM 14761 is Phascolarctobacterium faecium DSM 14762 is Phascolarctobacterium faecium
Similar to DSMZ Medium 104b, except glucose is omitted and sodium succinate is added.
DSMZ Medium 104b.5 -< for DSM 14760, DSM 14761 and DSM 14762
DSM 19022 is Lutispora thermophila DSM 19022
Similar to DSMZ Medium 104b, except glucose is ommitted and sodium pyruvate is added.
Similar to DSMZ Medium 104b, except glucose is omitted and vitamins and sodium glutamate (monosodium glutamate) are added.
DSM 21120 is Anaerosphaera aminiphila DSM 21120
Similar to DSMZ Medium 104b, except glucose is omitted and the pH is increased to 8.5.
DSM 21650 is Proteiniborus ethanoligenes DSM 21650
Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose is added. The pH is increased to 7.5-8.0.
DSM 21761 is Alkaliphilus oremlandii OhILAs
DSM 24749 is Youngiibacter fragilis 232.1
Similar to DSMZ Medium 104b, except glucose is omitted and D-fructose and sodium chloride are added. The pH is increased to 7.3-7.5.
DSM 26752 is Keratinibaculum paraultunense
Similar to DSMZ Medium 104b, except sodium carbonate is added and the pH is increased to 8.0.
Similar to DSMZ Medium 104b, except sodium carbonate is added and the pH increased to 8.5.
Similar to DSMZ Medium 104b, except glucose is omitted and D-cellobiose (beta-cellobiose) is added. The pH is lowered to 6.5.
Soil extract formed from garden soil. Formed by making a water extract of garden soil and sodium carbonate, followed by autoclaving and centrifugation.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium98.pdf 98. RHIZOBIUM MEDIUM Yeast extract 1.0 g Mannitol 10.0 g Agar 15.0 g Soil extract 200.0 ml Distilled water 800.0 ml Adjust pH to 7.0. Soil extract: Air-dried garden soil 80.0 g Na2CO3 0.2 g Distilled water 200.0 ml Autoclave soil suspension for one hour at 121˚C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase peptone, pepsi-digested peptone from meat (meat peptone), yeast extract, sodium chloride, salt solution, rezasurin, L-cysteine hydrochloride, D-glucose and sodium thiosulfate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104a.pdf 104a. ANAEROBACULUM THERMOTERRENUM MEDIUM Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g NaCl 8.0 g Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g D-Glucose 1.0 g Na2S2O3 x 5 H2O 2.5 g Distilled water 1000.0 ml Dissolve ingredients (except glucose, thiosulfate and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.0. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. After autoclaving add glucose and thiosulfate from sterile, anoxic stock solutions prepared under N2. Final pH of the completed medium should be 7.0. © 2007 DSMZ GmbH - All rights reserved
DSM strains: 11603 Aquamicrobium defluvii 15010 marine alpha proteobacterium V4.MO.26 16623 Bradyrhizobium canariense 11554 Bradyrhizobium elkanii USDA 76 1755 Bradyrhizobium japonicum 1756 Bradyrhizobium japonicum 1982 Bradyrhizobium japonicum 30131 Bradyrhizobium japonicum 19632 Bradyrhizobium jicamae 24092 Bradyrhizobium liaoningense 16931 Bradyrhizobium pachyrhizi 1984 Bradyrhizobium 5966 Bradyrhizobium 5968 Bradyrhizobium 5969 Bradyrhizobium 13375 Sinorhizobium arboris 13376 Sinorhizobium arboris 13377 Sinorhizobium arboris 5851 Sinorhizobium fredii 5852 Sinorhizobium fredii 5824 Sinorhizobium fredii 13372 Sinorhizobium kostiense 13373 Sinorhizobium kostiense 13374 Sinorhizobium kostiense 1981 Sinorhizobium meliloti 6057 Sinorhizobium meliloti 6057 Sinorhizobium meliloti 30135 Sinorhizobium meliloti 30136 Sinorhizobium meliloti 23913 Sinorhizobium meliloti 23914 Sinorhizobium meliloti 11273 Sinorhizobium saheli 26426 Ensifer sojae CCBAU 05684 11282 Sinorhizobium terangae 100023 Mesorhizobium camelthorni 17287 Mesorhizobium chacoense 1978 Mesorhizobium ciceri 29666 Mesorhizobium hawassense 6573 Mesorhizobium huakuii 2626 Mesorhizobium loti 2627 Mesorhizobium loti NZP2037 2628 Mesorhizobium loti 5235 Mesorhizobium loti 5236 Mesorhizobium loti 6046 Mesorhizobium loti 11555 Mesorhizobium mediterraneum UPM-Ca36 100038 Mesorhizobium muleiense 29667 Mesorhizobium qingshengii 100022 Mesorhizobium robiniae 100039 Mesorhizobium sangaii 29845 Mesorhizobium silamurunense 28320 Mesorhizobium tamadayense 11417 Mesorhizobium tianshanense 11587 Rhizobacter dauci 1111 Rhizobium aggregatum 11541 Rhizobium etli CFN 42 19332 Rhizobium fabae 11542 Neorhizobium galegae bv. orientalis str. HAMBI 540 11917 Rhizobium hainanense 26427 Rhizobium herbae 29977 Rhizobium laguerreae 1980 Rhizobium leguminosarum 2108 Rhizobium leguminosarum 6039 Rhizobium leguminosarum 6040 Rhizobium leguminosarum 6041 Rhizobium leguminosarum 6042 Rhizobium leguminosarum 6043 Rhizobium leguminosarum 6044 Rhizobium leguminosarum 6045 Rhizobium leguminosarum 30133 Rhizobium leguminosarum 30138 Rhizobium leguminosarum 30139 Rhizobium leguminosarum 30141 Rhizobium leguminosarum 30143 Rhizobium leguminosarum 1983 Bradyrhizobium lupini 30134 Bradyrhizobium lupini 30140 Bradyrhizobium lupini 28449 Rhizobium mesoamericanum CCGE 501 26482 Rhizobium petrolearium 1379 Rhizobium phaseoli 6038 Rhizobium phaseoli 30137 Rhizobium phaseoli 30132 Rhizobium pisi 26483 Rhizobium pseudoryzae 22668 Rhizobium pusense 26376 Rhizobium rosettiformans W3 26438 Rhizobium skierniewicense Ch11 1725 Rhizobium sp. AM1 2729 Rhizobium 2914 Rhizobium 6049 Rhizobium 6050 Rhizobium 6051 Rhizobium 6052 Rhizobium 6599 Rhizobium 10169 Rhizobium 11272 Rhizobium 11419 Rhizobium 14623 Rhizobium sullae 14624 Rhizobium sullae 11418 Rhizobium tropici 25379 Rhizobium tubonense 26437 Rhizobium vallis 25378 Rhizobium vignae 19334 Shinella kummerowiae 15007 Sinorhizobium americanum 15008 Sinorhizobium americanum
An organic-rich, solid culture medium containing yeast extract, mannitol, agar, and soil extract.
DSM strains: 3752 Haloarcula marismortui 11927 Haloarcula quadrata 3756 Haloarcula vallismortis 668 Halobacterium salinarum 669 Halobacterium salinarum 670 Halobacterium salinarum 671 Halobacterium salinarum 3754 Halobacterium salinarum 22414 Halobacterium salinarum 3753 Halobacterium sp. 3750 Halobacterium sp. 1307 Halococcus morrhuae DSM 1307 1308 Halococcus morrhuae 1309 Halococcus morrhuae 1310 Halococcus morrhuae 1411 Haloferax mediterranei ATCC 33500 3757 Haloferax volcanii DS2 15367 Halomonas magadiensis 1137 Halorubrum saccharovorum DSM 1137 13077 Natrialba aegyptia DSM 13077 3751 Natrinema pallidum DSM 3751
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium97.pdf 97. HALOBACTERIUM MEDIUM Casamino acids 7.50 g Yeast extract 10.00 g Na3-citrate 3.00 g KCl 2.00 g MgSO4 x 7 H2O 20.00 g FeSO4 x 7 H2O 0.05 g MnSO4 x H2O 0.20 mg NaCl 250.00 g Agar 20.00 g Distilled water 1000.00 ml Adjust pH to 7.4. Add the agar after dissolving all ingredients in the water and adjustment of pH. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of casamino acids, yeast extract, sodium citrate, potassium chloride, magnesium sulfate, ferrous sulfate, manganese sulfate, sodium chloride, and agar.
An organic-rich, liquid culture medium comprised of trypticase peptone, pepsin-digested peptone from meat (meat peptone), yeast extract, resazurin, salt solution, L-cysteine hydrochloride, sodium carbonate, and D-glucose. Prepared under an atmosphere of carbon dioxide and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104c.pdf 104c. PY + X MEDIUM (N2/CO2) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Resazurin 1.0 mg Salt solution (see medium 104) 40.0 ml Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 2.5 g D-Glucose 5.0 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine, carbonate and glucose), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Add cysteine, dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of autoclaved medium to 7.0 with an anoxic, sterile stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture. Prior to inoculation add glucose or any other substrate from a sterile, anoxic stock solution prepared under N2. For DSM 753 replace glucose with 10.0 g/l maltose. For DSM 7320 adjust pH to 6.3. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 104c, except glucose is omitted and maltose is added.
Similar to DSMZ Medium 104c, except the pH is lowered to 6.3.
An organic-rich, liquid culture medium comprised of trypicase peptone, pepsin-digested peptone from meat (meat peptone), yeast extract, salt solution, sodium chloride, magnesium sulfate, betaine (glycine betaine), resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium104d.pdf 104d. CLOSTRIDIUM HALOPHILUM MEDIUM (BETAINE) Trypticase peptone 5.0 g Peptone from meat (pepsin-digested) 5.0 g Yeast extract 10.0 g Salt solution (see medium 104) 40.0 ml NaCl 60.0 g MgSO4 x 7 H2O 5.0 g Betaine x H2O 6.0 g Resazurin 1.0 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and adjust pH to 7.3. Thereafter, dispense under 100% N2 gas atmosphere in culture vessels and autoclave. Adjust pH of completed medium to 7.3 – 7.5. © 2014 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium105.pdf 105. GLUCONOBACTER OXYDANS MEDIUM Glucose 100.0 g Yeast extract 10.0 g CaCO3 20.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8. 20 g calcium carbonate in the medium serves as a buffer. The calcium carbonate will settle in agar plates before the agar has set, producing an opaque layer in the bottom. As the strain grows and acid is produced this will react with the calcium carbonate, causing it to dissolve and form zones of clearing immediately below the colonies. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 27, except sodium malate (disodium malate), sodium thiosulfate, and sodium sulfide are added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium109.pdf 109. RHODOPSEUDOMONAS SULFOVIRIDIS MEDIUM To medium 27 add 0.1% sodium malate and 0.05% sodium thiosulfate. Adjust pH to 6.8 and sterilize at 121˚C for 15 min. The liquid medium is sterilized in rubber stoppered or screw-capped bottles under anaerobic conditions (Ref. 3365). After sterilization neutralized sterile sodium sulfide solution is added to a final concentration of 0.03% (see medium 27). © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of glucose, ammonium sulfate, potassium phossphate, magnesium sulfate, potassium chloride, calcium nitrate, ferrous sulfate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium108.pdf 108. THIOBACILLUS ACIDOPHILUS MEDIUM Glucose 10.00 g (NH4)2SO4 3.00 g KH2PO4 0.50 g MgSO4 x 7 H2O 1.00 g KCl 0.10 g Ca(NO3)2 x 4 H2O 18.00 mg FeSO4 x 7 H2O 0.01 mg Agar 15.00 g Distilled water 1000.00 ml In liquid medium, the pH should be adjusted to 3.5 with H2SO4. In solid medium, the pH should be adjusted to 4.5 with H2SO4 after autoclaving the medium. Sterilize the glucose and the basal solution separately. © 2007 DSMZ GmbH - All rights reserved
Sludge fluid where yeast extract has been added, and has been gassed with dinitrogen, then centrifuged and autoclaved.
A fatty acid solution comprised of valeric acid, isovaleric acid, 2-methylbutyric acid, and isobutyric acid.
A defined organic solution containing short-chain fatty acids or short-chain fatty acid anions.
An organic-rich, liquid culture medium comprised of casitone, yeast extract, D-glucose, cellobiose, maltose, sodium lactate, clarified rumen fluid, potassium phosphate, ammonium sulfate, sodium chloride, calcium chloride, magnesium sulfate, sodium carbonate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared over an atmosphere of carbon dioxide and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium1310.pdf 1310. BUTYRIVIBRIO M2 MEDIUM Casitone (BACTO) 10.00 g Yeast extract 2.50 g D-Glucose 2.00 g Cellobiose 2.00 g Maltose 2.00 g Na-DL-lactate 5.00 g Rumen fluid, clarified (see below) 200.00 ml K2HPO4 0.05 g KH2PO4 0.05 g (NH4)2SO4 0.10 g NaCl 0.10 g CaCl2 x 2 H2O 0.01 g MgSO4 x 7 H2O 0.01 g Resazurin 0.50 mg Na2CO3 4.00 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 800.00 ml Dissolve ingredients (except carbonate, cysteine and sulfide), boil medium for 1 min, then cool to room temperature under 100% CO2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Add cysteine and sulfide form sterile anoxic stock solutions prepared under 100% N2 gas. Adjust the final pH of the medium to 6.5 – 6.8. Clarified rumen fluid: Rumen fluid from cow or sheep is filtered through muslin, autoclaved at 121 °C for 15 min. and then centrifuged at 27,000 g for 20 min. The supernatant is made anoxic by flushing with nitrogen for 15 min. and then stored frozen at -20 °C. © 2014 DSMZ GmbH - All rights reserved
clarified rumen fluid for DSMZ Medium 1310
Clarified rumen fluid that is filtered through muslin, made anoxic by flusing with dinitrogen.
An inorganic salts solution comprised of calcium chloride, magnesium sulfate, potasium phosphate, sodium bicarbonate, and sodium chloride. Has a salinity of 1.475%.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium143.pdf 143. CLOSTRIDIUM SP. LA1 MEDIUM Crotonic acid 6.00 g NaOH 0.30 g (NH4)2HPO4 0.15 g K2HPO4 0.10 g MgCl2 x 6 H2O 0.03 g CaCl2 x 2 H2O 0.04 g NH4Cl 0.05 g MgSO4 x 7 H2O 0.60 mg MnSO4 x 2 H2O 0.40 mg FeSO4 x 7 H2O 0.40 mg (NH4)6Mo7O24 x 4 H2O 10.00 mg Biotin 0.04 mg p-Aminobenzoic acid 0.80 mg Tryptone (Difco) 1.00 g Yeast extract 1.00 g Resazurin 1.00 mg K2CO3 4.00 g Distilled water 1000.00 ml Dissolve ingredients (except K2CO3) and sparge medium with 100% N2 gas to make it anoxic (30 – 45 min). An anoxic stock solution of the potassium carbonate is autoclaved separately under 80% N2 and 20% CO2 gas mixture and thereafter added to the sterile medium. Adjust pH of the completed medium to 6.8 - 7.0. Prior to inoculation add 10 - 20 mg/l sodium dithionite from a 5% (w/v) stock solution that has been freshly prepared under N2 and sterilized by filtration. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of crotonic acid, sodium hydroxide, ammonium phosphate, potassium phosphate, magnesium chloride, calcium chloride, ammonium chloride, magnesium sulfate, manganese sulfate, ferrous suflate, ammonium molybdate, p-aminobenzoic acid (4-aminobenzoic acid), tyrptone, yeast extract, resazurin, sodium dithionite, and potassium carbonate. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 144, except D-glucose is omitted.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium144.pdf 144. THERMOANAEROBIUM MEDIUM NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trace element solution (see below) 9.00 ml FeSO4 x 7 H2O 3.00 mg Yeast extract 3.00 g Tryptone or Trypticase 10.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 5.00 ml D-Glucose 5.00 g Na2S x 9 H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, glucose and sulfide), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Dispense under same gas atmosphere in culture vessels and autoclave. Add glucose, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under N2. Adjust pH of final medium to 7.2 - 7.4. For DSM 7040 omit tryptone and reduce amount of yeast extract to 1.00 g/l. For DSM 12299 omit D-glucose. Trace element solution: Nitrilotriacetic acid 12.80 g FeCl2 x 4 H2O 0.20 g MnCl2 x 4 H2O 0.10 g CoCl2 x 6 H2O 0.17 g CaCl2 x 2 H2O 0.10 g ZnCl2 0.10 g CuCl2 0.02 g H3BO3 0.01 g Na2MoO4 x 2 H2O 0.01 g NiCl2 x 6 H2O 26.00 mg NaCl 1.00 g Na2SeO3 x 5 H2O 0.02 g Distilled water 1000.00 ml First, dissolve nitrilotriacetic acid and adjust to pH 6.5 with KOH. © 2015 DSMZ GmbH - All rights reserved
DSM 12299 is Thermoanaerobacter siderophilus SR4
Similar to DSMZ Medium 144, except tryptone is omitted and the concentration of yeast extract is decreased.
DSM 7040 is Caldicellulosiruptor acetigenus DSM 7040
An organic-rich, liquid culture medium comprised of ammonium chloride, sodium chloride, magnesium chloride, potassium phosphate, trace elements, ferrous sulfate, yeast extract, tryptone (or trypicase peptone), resazurin, vitamins, D-glucose, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
Similar to DSMZ Medium 142, except cobalamin is omitted and the pH is increased to 7.3-7.6.
DSM 12346 is Thiomicrospira crunogena DSM 12350 is Thiomicrospira kuenenii DSM 12350 DSM 12351 is Thiomicrospira frisia DSM 12352 is Thiomicrospira chilensis DSM 12352 DSM 12353 is Thiomicrospira crunogena DSM 12354 is Thiomicrospira crunogena
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium142.pdf 142. THIOMICROSPIRA PELOPHILA MEDIUM NaCl 25.00 g (NH4)2SO4 1.00 g MgSO4 x 7 H2O 1.50 g CaCl2 x 2 H2O 0.42 g Trace element solution (see medium 69) 0.20 ml Bromothymol blue 4.00 mg K2HPO4 0.50 g Na2S2O3 x 5 H2O 5.00 g Vitamin B12 15.00 μg Distilled water 1000.00 ml Adjust pH to 7.2. K2HPO4 and Na2S2O3 are autoclaved separately each in 10% of the final volume. Filter sterilize the vitamin B12. Adjust the completed medium to pH 7.2 with sterile 0.4% (w/v) Na2CO3 solution. Alternatively, "aged sea water" may be used (see medium 246), supplemented by the indicated amounts of (NH4)2SO4, bromothymol blue, K2HPO4, sodium thiosulfate and vitamin B12. Growth of most strains of Thiomicrospira is more reliable if the medium is prepared under a 80% N2 and 20% CO2 gas atmosphere to make it anoxic and then filled under air atmosphere in Hungate-type tubes (5 ml per vial). The pH is adjusted with a sterile stock solution of NaHCO3 (10% w/v) after autoclaving. For DSM 12346, DSM 12350, DSM 12351, DSM 12352, DSM 12353, and DSM 12354 omit vitamins and adjust pH of final medium to 7.3 – 7.6. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised sodium chloride, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, bromothymol blue, potassium phosphate, sodium thiosulfate, and cobalamin. Medium has midly reducing redox if prepared under dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium142a.pdf 142a. THIOMICROSPIRA PSYCHROPHILA MEDIUM NaCl 15.00 g (NH4)2SO4 1.00 g MgSO4 x 7 H2O 1.50 g CaCl2 x 2 H2O 0.42 g Trace element solution SL-10 (see medium 320) 1.00 ml Bromothymol blue 4.00 mg K2HPO4 0.50 g Na2S2O3 x 5 H2O 5.00 g Seven vitamins solution (see medium 503) 1.00 ml Distilled water 1000.00 ml Dissolve ingredients (except phosphate, thiosulfate and vitamins), adjust pH to 6.0 and autoclave. Phosphate and thiosulfate are autoclaved separately each in 5% of the final volume. The vitamin solution is sterilized by filtration. Adjust the completed medium to pH 7.6 with sterile 5% (w/v) NaHCO3 solution. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium chloride, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, bromothymol blue, potassium phosphate, sodium thiosulfate, and vitamins.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141c.pdf 141c. GTA13 MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Trimethylammonium chloride 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylammonium chloride, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% N2 and 20% CO2 gas mixture. After sterilization add trimethylammonium chloride, cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 – 7.5. For DSM 2834, DSM 3028, DSM 3029, DSM 3318, DSM 4659, DSM 6636, DSM 13159, DSM 15192, DSM 21213, and DSM 21339 replace trimethylammonium chloride by 5.00 ml/l methanol added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of final medium to 6.8 – 7.2. For DSM 5309 omit trimethylammonium chloride from the medium. For DSM 6564 adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 141c, except the pH is reduced to 6.8.
DSM 6564 is Methanosarcina siciliae HI350
Similar to DSMZ Medium 141c, except trimethylammonium is omitted.
DSM 2834 is Methanosarcina acetivorans C2A DSM 3028 is Methanosarcina siciliae T4/M DSM 3029 is Methanolobus vulcani DSM 3318 is Methanosarcina mazei C16 DSM 4659 is not in www.dsmz. de. Is Methanosarcina sp. WH1 in StrainInfo. DSM 6636 is Methanosarcina sp. MTP4 DSM 13159 is Methanosarcina calensis str. Cali DSM 15192 is Methanosarcina sp. DSM 21213 is Methanolobus profundi DSM 21339 is Methanolobus zinderi
Similar to DSMZ Medium 141c, except trimethylammonium chloride is omitted and ethanol added. The pH is reduced to 6.8-7.2.
An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, trimethylammonium chloride, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141b.pdf 141b. METHANOCULLEUS SP. MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 6.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (OXOID) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 6.8 – 7.0. If the medium is being used without gas mixture overpressure then adjust pH with a small amount of sterile anoxic 1 N HCl, if necessary. © 2014 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium141a.pdf 141a. METHANOCOCCUS SP. MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Resazurin 1.00 mg Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except vitamins, cysteine and sulfide), sparge medium with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclave anoxically under the same gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 5.5 – 5.7. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium chloride, manganese chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, ammonium acetate, resazurin, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium140.pdf 140. CLOSTRIDIUM CELLOBIOPARUM MEDIUM KH2PO4 0.50 g NaCl 1.00 g (NH4)2 SO4 0.50 g MgSO4 x 7 H2O 0.10 g CaCl2 x 2 H2O 0.10 g K2HPO4 0.50 g Clarified rumen fluid or sludge fluid (see medium 119) 300.00 ml Cellobiose 5.00 g NaHCO3 10.00 g Resazurin 1.00 mg L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 700.00 ml Dissolve ingredients (except bicarbonate, cellobiose and reducing agents), bring medium to the boil, then cool to room temperature under 100% CO2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. Add cellobiose, sulfide and cysteine from sterile anoxic stock solutions prepared under N2 and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Cellobiose is sterilized by filtration. Adjust pH of completed medium to 6.8. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, ammonium sulfate, mangesium sulfate, calcium chloride, clarified rumen fluid or sludge fluid, cellobiose, sodium bicarbonate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium139.pdf 139. CLOSTRIDIUM HISTOLYTICUM MEDIUM Trypticase soy broth (BBL) 15.00 g Proteose peptone (Difco) 50.00 g Na2HPO4 9.00 g KH2PO4 1.92 g MgSO4 x 7 H2O 0.08 g Distilled water 1000.00 ml Dissolve ingredients, adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase soy broth (nn organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), dextrose (D-glucose), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate), proteose peptone, sodium phosphate, potassium phosphate, and magnesium sulfate.
An organic-rich, liquid culture medium comprised of yeast extract and Lindane (gamma-hexachlorocyclohexane).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium138.pdf 138. LINDANE MEDIUM Yeast extract 5.0 g Hexachlorocyclo-hexane (Lindane) 0.1 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase (trypticase peptone), yeast extract, sodium lactate, sodium thioglycolate, tween 80 (polysorbate 80), glucose, putrescine, and resazurin.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium136.pdf 136. VEILLONELLA MEDIUM Trypticase (BBL) 5.00 g Yeast extract 3.00 g Na-(DL)-lactate 7.50 g Na-thioglycolate 0.75 g Tween 80 1.00 g Glucose 1.00 g Putrescine 3.00 mg Resazurin 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.5 with solid K2CO3. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium lactate, potassium phosphate, ammonium chloride, magnesium sulfate, sodium chloride, calcium chloride, and trace elements.
mineral lactate medium (low carbon content)
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium137.pdf 137. MINERAL LACTATE MEDIUM (low carbon content) Na-lactate 0.50 g K2HPO4 x 3 H2O 1.13 g KH2PO4 0.88 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.50 g NaCl 1.00 g CaCl2 x 2 H2O 5.00 mg Trace element solution (see medium 69) 1.20 ml Distilled water 1000.00 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium carbonate, sodium syringate, vitamins, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135b.pdf 135b. ACETOBACTERIUM DEHALOGENANS MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg Na2CO3 5.00 g Na-syringate 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, syringate, vitamins and cysteine, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Thereafter, dispense in anoxic culture vessels under same gas atmosphere and autoclave. Add syringate, vitamins and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the completed medium should be at 7.4. After growth has been started add an additional amount of syringate to reach a final concentration of 2.00 g/l. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium bicarbonate, ethylene glycol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135a.pdf 135a. ACETOBACTERIUM MEDIUM (MARINE) NaCl 20.00 g NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 5.00 g Ethylene glycol 1.25 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, ethylene glycol, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use add ethylene glycol, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2 gas. Adjust pH of completed medium to 7.0 – 7.2. © 2015 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium135.pdf 135. ACETOBACTERIUM MEDIUM NH4Cl 1.00 g KH2PO4 0.33 g K2HPO4 0.45 g MgSO4 x 7 H2O 0.10 g Trace element solution (see medium 141) 20.00 ml Yeast extract 2.00 g Resazurin 1.00 mg NaHCO3 10.00 g D-Fructose 10.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, fructose, vitamins, cysteine and sulfide, bring to the boil and cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add bicarbonate (solid) and equilibrate the medium with the gas until a pH of around 7.4 is reached. Then distribute and autoclave under the same gas atmosphere. Before use adjust the pH to 8.2 by adding a sterile anoxic stock solution of carbonate (5% w/v) prepared under 80% N2 and 20% CO2 gas mixture (c. 0.25 ml per 10 ml medium) and add fructose, vitamins (sterilized by filtration), cysteine and sulfide from anoxic sterile stock solutions prepared under 100% N2. For autotrophic growth fructose is omitted and a gas atmosphere of 80% H2 and 20% CO2 is used. For DSM 1974 and DSM 7417 use only 1.00 g/l of NaHCO3 and omit Na2CO3 to reach a final pH of 6.5. For DSM 4132 use fructose as substrate at a final concentration of 1.00 g/l. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 135, except the concentration of fructose is decreased.
Similar to DSMZ Medium 135, except sodium carbonate is omitted and the concentration of sodium bicaronbate is reduced. The pH is reduced to 6.5.
DSMZ Medium 135.1 -< for DSM 1974 and DSM 7417
DSM 1974 is Moorella thermoautotrophica DSM 7417 is Moorella thermoautotrophica
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, yeast extract, resazurin, sodium bicarbonate, D-fructose, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide. If fructose is not used, dihydrogen is used in the atmosphere.
Similar to DSMZ Medium 133, except para-aminobenzoic acid (4-aminobenzoic acid) is added. For chemoorganotrophic growth, sodium pyruvate and yeast extract are also added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium133.pdf 133. CARBON MONOXIDE OXIDIZER MEDIUM Na2HPO4 x 12 H2O 4.500 g KH2PO4 0.750 g NH4Cl 1.500 g MgSO4 x 7 H2O 0.200 g CaCl2 x 2 H2O 0.030 g Ferric ammonium citrate 0.018 g Trace element solution SL-6 (see medium 27) 1.000 ml Agar (if necessary) 12.000 g Distilled water 1000.000 ml Adjust pH to 7.0. For chemoautotrophic growth incubate under a gas atmosphere of a) 20 - 80% CO + 10% O2 + 70 - 10% N2 or b) 70% H2 + 20% O2 + 10% CO2 adding 2.5 g NaHCO3 per liter of medium. For chemoorganotrophic growth add 3.0 g sodium acetate and incubate under air. For DSM 1083 the medium has to be supplemented with 10 ml/l of the vitamin solution of medium 141, sterilized by filtration. For chemoorganotrophic growth with acetate under air add also 10 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 1085 the medium has to be supplemented with 20 μg/l vitamin B12. For chemoorganotrophic growth with acetate under air add also 20 ml/l of a 5% w/v NaHCO3 solution, sterilized by filtration. For DSM 13294 the medium has to be supplemented with 50 μg/l paraaminobenzoic acid. For chemoorganotrophic growth under air add also 2 g/l Na-pyruvate and 1 g/l yeast extract. © 2009 DSMZ GmbH - All rights reserved
DSM 1085 is Methyloversatilis universalis
Similar to DSMZ Medium 133, except vitamin B23 (cobalamin) is added. For chemoorganotrophic growth, sodium bicarbonate is also added.
Similar to DSMZ Medium 133, except vitamins are added. If grown under chemoorganotrophic growth, sodium bicarbonate is added.
DSM 1083 is Pseudomonas carboxydohydrogena
A minerals-salts, solid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, ferric ammonium citrate, trace elements, and agar. Prepared under an atmosphere of carbon monoxide, dioxygen, and dinitrogen. Or prepared under an atmosphere of dihydrogen, dioxygen, and carbon dioxide, with sodium bicarbonate. Or, sodium acetate is added and is prepared under an atmosphere of air (dioxygen).
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, calcium chloride, magnesium sulfate, ferric trichloride, manganese sulfate, trace elements, glutamic acid, trypticase soy broth without glucose (An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), papaic digest of soybean (soy peptone), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate)), thamine hydrochloride, vitamin B12 (cobalamin), and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium134.pdf 134. HALISCOMENOBACTER MEDIUM KH2PO4 27.00 mg K2HPO4 40.00 mg Na2HPO4 x 2 H2O 40.00 mg CaCl2 x 2 H2O 50.00 mg MgSO4 x 7 H2O 75.00 mg FeCl3 x 6 H2O 5.00 mg MnSO4 x H2O 3.00 mg Trace element solution SL-6 (see medium 27) 1.00 ml Glutamic acid 1.31 g Trypticase soy broth without glucose (BBL) 2.50 mg Thiamine-HCl x 2 H2O 0.40 mg Vitamin B12 0.01 mg Glucose 2.00 g Distilled water 1000.00 ml Adjust pH to 7.5. The vitamins are sterilized by filtration. The glucose is dissolved in 5 ml of distilled water and sterilized separately by autoclaving. © 2007 DSMZ GmbH - All rights reserved
129. POTATO DEXTROSE AGAR Infusion from potatoes (see below) 1000.0 ml Glucose 20.0 g Agar 15.0 g Potato infusion: Boil 200 g scrubbed and sliced potatoes in 1000 ml water for 1 hour. Pass through fine sieve. Avoid new potatoes. © 2007 DSMZ GmbH - All rights reserved
A water extract of potatoes (Solanum tuberosum), prepared by boiling then filtering the aqueous extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium129.pdf 129. POTATO DEXTROSE AGAR Infusion from potatoes (see below) 1000.0 ml Glucose 20.0 g Agar 15.0 g Potato infusion: Boil 200 g scrubbed and sliced potatoes in 1000 ml water for 1 hour. Pass through fine sieve. Avoid new potatoes. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of potato infusion, glucose, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium130.pdf 130. CZAPEK-DOX AGAR Sucrose 30.00 g NaNO3 3.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g FeSO4 x 7 H2O 0.01 g K2HPO4 1.00 g Agar 13.00 g Distilled water 1000.00 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of sucrose, sodium nitrate, magnesium sulfate, potassium chloride, ferrous sulfate, potassium phosphate, and agar.
An organic-rich, solid culture medium comprised of glucose, soluble starch, yeast extract, casitone, calcium carbonate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium127.pdf 127. MICROMONOSPORA MEGALOMICEA MEDIUM Glucose 10.0 g Soluble starch 20.0 g Yeast extract 5.0 g Casitone 5.0 g CaCO3 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1, except glycerol is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium126.pdf 126. MYCOBACTERIUM INTRACELLULARE MEDIUM To medium 1 add 20 g glycerol. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium124.pdf 124. DESULFOTOMACULUM ACETOXIDANS MEDIUM NaCl 1.17 g MgCl2 x 6 H2O 0.40 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-acetate 1.40 g Na-butyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under N2. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.0 - 7.2, if necessary. For DSM 2925 replace acetate and butyrate with 1.00 g/l ethanol added from a sterile anoxic stock solution after autoclaving. For DSM 7213 replace acetate and butyrate with 0.60 g/l Na-benzoate added from a sterile anoxic stock solution after autoclaving. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 124, except sodium acetate and sodium butyrate are omitted and sodium benzoate is added.
DSM 7213 is Desulfotomaculum gibsoniae DSM 7213
Similar to DSMZ Medium 124, except sodium acetate and sodium butyrate are omitted and ethanol is added.
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, potassium chloride, calcium chloride, ammonium chloride, potassium phosphate, sodium sulfate, trace elements, selenite-tungstate, sodium acetate, sodium butyrate, yeast extract, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
A minerals-salts, solid culture medium comprised of potassium nitrate, magnesium sulfate, calcium chloride, sodium phosphate, ferrous sulfate, copper sulfate, boric acid, manganese sulfate, zinc sulfate, molybdenum trioxide, and agar. Prepared with methanol or an atmosphere of methane and air (dioxygen).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium125.pdf 125. METHYLOBACTERIUM MEDIUM KNO3 1.00 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.02 g Na2HPO4 0.23 g NaH2PO4 0.07 g FeSO4 x 7 H2O 1.00 mg CuSO4 x 5 H2O 5.00 μg H3BO3 10.00 μg MnSO4 x 5 H2O 10.00 μg ZnSO4 x 7 H2O 70.00 μg MoO3 10.00 μg Agar 12.00 g Distilled water 1000.00 ml Add 5 ml methanol or incubate under methane-air (4:1). Adjust pH to 6.8. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium123.pdf 123. MARINE AGAR To 1000 ml "Synthetic sea water" (see medium 79) add 5 g Bacto Peptone, 1 g Bacto Yeast extract, and 15 g agar. Alternatively medium 514 may be used. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of synthetic sea water, peptone, yeast extract, and agar.
An organic-rich, liquid culture medium comprised of sodium aspartate, yeast extract, magnesium sulfate, calcium chloride, potassium phosphate, sodium phosphate, cysteine hydrochloride, resazurin, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium121.pdf 121. MEDIUM FOR CAMPYLOBACTER DSM 806 Na-aspartate 10.00 g Yeast extract 0.20 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 28.00 mg K2HPO4 0.75 g NaH2PO4 0.25 g Cysteine-HCl x H2O 0.25 g Resazurin 1.00 mg Trace element solution 1.00 ml SL-10 (see medium 320) Distilled water 1000.00 ml Adjust pH to 7.0. Phosphate and cysteine should be dissolved together in 1/10 vol. of water and sterilized separately. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, magnesium chloride, potassium phosphate, calcium chloride, sodium beta-glycerol phosphate (sodium glycerol-2-phosphate), ferrous sulfate, L-glutathione (glutathione), yeast extract, resazuring, and cellulose or cellobiose. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium122.pdf 122. CLOSTRIDIUM THERMOCELLUM MEDIUM (NH4)2SO4 1.30 g MgCl2 x 6 H2O 2.60 g KH2PO4 1.43 g K2HPO4 5.50 g CaCl2 x 2 H2O 0.13 g Na2-ß-glycerol phosphate x 4 H2O 6.00 g FeSO4 x 7 H2O 1.10 mg L-Glutathione reduced 0.25 g Yeast extract 4.50 g Resazurin 1.00 mg Cellulose (Avicel or MN 300) 10.00 g or Cellobiose 5.00 g Distilled water 1000.00 ml Prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 7.0 - 7.2 and autoclave. Cellobiose is added after autoclaving from an anoxic and filter-sterilized stock solution of 10% (w/v). A white precipitate forms after mixing the ingredients of this medium, but this has no negative effect on growth. © 2009 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120b.pdf 120b. METHANOMICROCOCCUS MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.50 g Coenzyme M (2-mercaptoethanesulfonate) 0.14 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.36 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, coenzyme M, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% H2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 7.2 and dispense medium under 80% H2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins and coenzyme M are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 7.0 – 7.2. After inoculation, pressurize the gas head space of culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. For DSM 22503 supplement medium with 6.0 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 120b, except the concentration of sodium chloride is increased.
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, coenzyme M, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120c.pdf 120c. GÖ1 MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 10.00 g Sucrose 17.10 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, sucrose, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 120a.2 -< for DSM 10334 and DSM 13486
Similar to DSMZ Medium 120a, except the concentration of sodium bicarbonate is increased and the concentration of methanol is decreased.
DSM 10334 is Methanosarcina lacustris Z-7289 DSM 13486 is Methanosarcina lacustris ZS
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium120.pdf 120. METHANOSARCINA MAZEI MEDIUM K2HPO4 0.35 g KH2PO4 0.23 g NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract (Difco) 2.00 g Casitone (Difco) 2.00 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Then add and dissolve bicarbonate, adjust pH to 6.8 and dispense medium under 80% N2 and 20% CO2 gas atmosphere in culture vessels and autoclave. Methanol (50% v/v) and the reducing agents are each autoclaved separately under N2 gas atmosphere as concentrated solutions in tightly closed tubes, vitamins are prepared under 100% N2 and sterilized by filtration. Appropriate volumes of the solutions are injected into the autoclaved main part of the medium with hypodermic syringes. Final pH of the complete medium is 6.8 – 7.0. For DSM 1825, DSM 7081, and DSM 11855 supplement medium with 5% (v/v) rumen fluid, clarified (see medium 1310) and adjust pH to 6.5 – 6.8. For DSM 7058 and DSM 9195 replace methanol with 5.00 g/l trimethylamine-HCl. For DSM 21571 supplement medium with 6.00 g/l NaCl. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 120, except the concentration of sodium chloride is increased.
DSM 21571 is Methanosarcina horonobensis
DSMZ Medium 120.2 -< for DSM 7058 and DSM 9195
DSM 7058 is Methanosarcina mazei DSM 9195 is Methanosarcina mazei TMA
Similar to DSMZ Medium 120, except methanol is omitted and trimethylamine hydrochloride is added.
DSM 1825 is Methanosarcina thermophila TM-1 DSM 7081 is Methanosarcina thermophila DSM 11855 is Methanosarcina thermophila
Similar to DSMZ Medium 120, except clarified rumen fluid is added, and the pH is decreased slightly to 6.5-6.8.
DSMZ Medium 120.1 -< for DSM 1825, DSM 7081, and DSM 11855
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, yeast extract, casitone, sodium acetate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
trace elements solution for DSMZ Medium 144
A trace elements solution comprised of nitrilotriacetic acid, ferrous chloride, manganese chloride, cobalt chloride, calcium chloride, zinc chloride, copper chloride, boric acid, sodium molybdate, nickel chloride, sodium chloride, and sodium selenite.
An organic-rich, liquid culture medium comprised of glucose, yeast extract, trypticase (trypticase peptone) agar, and potassium phosphate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium168.pdf 168. SPIROCHAETA AURANTIA MEDIUM Solution A: Glucose 2.0 g Yeast extract 2.0 g Trypticase (BBL) 5.0 g Agar (Bacto, Difco) 10.0 g (if necessary) Distilled water 1000.0 ml Adjust pH to 7.5 with KOH. Solution B: 1 M K-phosphate-buffer, pH 7.0 10.0 ml Sterilize part A and B separately by autoclaving at 121˚C for 15 min and combine thereafter. Final pH: 7.0 - 7.3. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of skim milk (powder), magnesium sulfate, potassium nitrate, sodium chloride, ferric citrate, neopeptone, glycerol, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium146.pdf 146. HALOCOCCUS MEDIUM Solution A: Skim milk (Difco) 50.0 g Distilled water 500.0 ml Solution B: MgSO4 x 7 H2O 10.0 g KNO3 2.0 g NaCl 200.0 g Fe-citrate trace - Distilled water 100.0 ml Solution C: Neopeptone 5.0 g Glycerol 10.0 g Agar 25.0 g Distilled water 400.0 ml Steam the three solutions separately, mix together solutions B and C and adjust pH to 8.4. Autoclave the skim milk at 112˚C for 15 min., and the agar salt mixture at 121˚C for 20 min. Add the hot mixture to the warm skim milk aseptically. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium147.pdf 147. RUMINOBACTER AMYLOPHILUS MEDIUM K2HPO4 0.45 g KH2PO4 0.45 g (NH4)2SO4 0.90 g NaCl 0.90 g MgSO4 x 7 H2O 0.18 g CaCl2 x 2 H2O 0.12 g Starch, soluble 5.00 g Casitone (Difco) 10.00 g Resazurin 1.00 mg Cysteine-HCl x H2O 0.50 g NaHCO3 6.00 g Distilled water 1000.00 ml Adjust pH to 7.0. Gas atmosphere: 100% CO2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magensium sulfate, calcium chloride, soluble starch, casitone, resazurin, cysteine hydrochloride, and sodium bicarbonate. Prepared under an atmosphere of carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium148.pdf 148. DESULFUROMONAS SP. MEDIUM KH2PO4 1.0 g NH4Cl 0.5 g MgSO4 x 7 H2O 0.4 g CaCl2 x 2 H2O 0.1 g Trace element solution SL-10 (see medium 320) 1.0 ml Selenite-tungstate solution (see medium 385) 1.0 ml Na-acetate 0.5 g Fumaric acid 1.5 g Resazurin 0.5 mg NaHCO3 2.5 g Vitamin solution (see medium 503) 1.0 ml Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide), bring to the boil, then cool to room temperature under 100% N2 gas and dispense under same gas atmosphere in anoxic tubes or bottles. After autoclaving complete the medium by adding residual substances from separately autoclaved (vitamins are sterilized by filtration) anoxic stock solutions prepared under N2 gas or 80% N2 and 20% CO2 gas atmosphere (bicarbonate). Adjust pH of the completed medium to 7.0-7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ptoassium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, trace elements, selenite-tungstate, sodium acetate, fumaric acid, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium149.pdf 149. DESULFOVIBRIO GIGAS MEDIUM Solution A: KH2PO4 1.0 g NH4Cl 0.5 g MgSO4 x 7 H2O 0.4 g Na2SO4 2.0 g CaCl2 x 2 H2O 0.1 g Trace element solution SL-6 (see medium 27) 1.0 ml 2 M H2SO4 1.0 ml Na-L-lactate 2.0 g Distilled water 950.0 ml Solution B: NaHCO3 2.0 g Distilled water 40.0 ml Solution C: Vitamin solution (see medium 503) 1.0 ml Solution D: Na2S x 9 H2O 0.3 g Distilled water 10.0 ml Solution A is gassed with 100% N2 gas to make it anoxic (at least 30 min) and then autoclaved under the same gas mixture. Solution D is autoclaved separately under 100% N2 gas. Solution B is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under N2 gas atmosphere and sterilized by filtration. Solutions B to D are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.0 - 7.2. Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, sodium sulfate, calcium chloride, trace elements, sulfuric acid, sodium lactate, sodium bicarbonate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide.
A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, yeast extract, elemental sulfur, and sulfuric acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150.pdf 150. ACIDIANUS BRIERLEYI MEDIUM (NH4)2SO4 3.00 g K2HPO4 x 3 H2O 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g Yeast extract 0.20 g Sulfur (flowers) 10.00 g Distilled water 1000.00 ml Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days. For DSM 18786 adjust pH to 0.8. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 150, except the pH is decreased to 0.8.
A minerals-salts culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, elemental sulfur, trace elements, and sulfuric acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium150a.pdf 150a. THIOBACILLUS CALDUS MEDIUM Use medium 150 without yeast extract. Adjust pH to 2.5 with sulfuric acid. After autoclaving add 10 ml/l of filter-sterilized trace elements solution (see below) and 5 g/l of sulfur powder (sterilized by steaming for 3 hours on each 3 successive days). Trace element solution: FeCl3 x 6 H2O 11.0 mg CuSO4 x 5 H2O 0.5 mg H3BO3 2.0 mg MnSO4 x H2O 2.0 mg Na2MoO4 x 2 H2O 0.8 mg CoCl2 x 6 H2O 0.6 mg ZnSO4 x 7 H2O 0.9 mg Distilled water 10.0 ml © 2010 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium153.pdf 153. THIOGLYCOLATE MEDIUM (MERCK 108190) Trypticase (BBL) 15.00 g L-Cystine 0.50 g Glucose 2.00 g Yeast extract 5.00 g NaCl 2.50 g Na-thioglycolate 0.50 g Resazurin 0.50 mg Distilled water 1000.00 ml Dissolve ingredients, sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic. Adjust pH to 7.1 with Na2CO3 (10% w/v), if necessary. Then dispense medium in Hungate-type tubes under the same gas atmosphere and autoclave. For solid medium add 15.00 g/l agar prior to autoclaving. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of trypticase (trypticase peptone), L-cystine, glucose, yeast extract, sodium chloride, sodium thioglycolate, and resazurin. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium155.pdf 155. BEGGIATOA MEDIUM CaSO4 (saturated solution) 20.000 ml NH4Cl 0.450 mg K2HPO4 0.100 mg MgSO4 x 7 H2O 0.200 mg Trace element solution (see below) 5.000 ml Na-acetate 0.500 g Nutrient broth (Difco) 0.500 g Agar (if necessary) 10.000 g Catalase, filter-sterilized 15000 – 35000 units/1000 ml Distilled water 1000.000 ml Adjust pH to 7.4 using pH indicator paper before autoclaving. The catalase is added just before inoculation. Trace element solution: EDTA 0.200 g FeSO4 x 7 H2O 0.700 g ZnSO4 x 7 H2O 0.010 g MnSO4 x 4 H2O 0.002 g CuSO4 x 5 H2O 5.000 μg H3BO3 10.000 mg Co(NO3)2 1.000 mg Na2MoO4 x 2 H2O 1.000 mg Distilled water 1000.000 ml © 2009 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of calcium sulfate, ammonium chloride, potassium phosphate, magnesium sulfate, trace elements, sodium acetate, nutrient broth (peptone and beef extract), agar, and catalase.
trace elements solution for DSMZ Medium 155
A trace elements solution comprised of EDTA, ferrous suflate, zinc sulfate, manganese sulfate, copper sulfate, boric acid, cobalt nitrate, and sodium molybdate.
An organic-rich, liquid culture medium comprised of L-alanine, peptone, yeast extract, cysteine hydrochloride, magnesium sulfate, ferrous sulfate, potassium phosphate, calcium sulfate, resazurin, and sodium bicarbonate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium156.pdf 156. CLOSTRIDIUM PROPIONICUM MEDIUM L-Alanine 3.0 g Peptone 3.0 g Yeast extract 4.0 g Cysteine-HCl x H2O 0.3 g MgSO4 x 7 H2O 0.1 g FeSO4 x 7 H2O 18.0 mg Potassium phosphate buffer (1 M, pH 7.1) 5.0 ml CaSO4 (satur. sol.) 2.5 ml Resazurin 1.0 mg NaHCO3 1.0 g Distilled water 1000.0 ml Dissolve ingredients (except bicarbonate), adjust pH to 7.0, then sparge medium with 100% N2 gas to make it anoxic (ca. 30 – 45 min). Add and dissolve 1.0 g/l NaHCO3 to the medium, then dispense under N2 gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium sulfate, magnesium sulfate, calcium chloride, trace elements, yeast extract, glucose, and sulfuric acid.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium158.pdf 158. THERMOPLASMA ACIDOPHILUM MEDIUM (NH4)2SO4 1.320 g KH2PO4 0.372 g MgSO4 x 7 H2O 0.247 g CaCl2 x 2 H2O 0.074 g Trace element solution (see below) 10.000 ml Yeast extract (Difco) 2.000 g Glucose 20.000 g Distilled water 1000.000 ml (freshly distilled) Adjust pH to 1.0 with 1 N H2SO4. Stock solutions of yeast extract (10% w/v) and glucose (50% w/v) in water are sterilized separately by autoclaving and thereafter added to the sterile mineral salt medium. Trace element solution: FeCl3 x 6 H2O 1.930 g MnCl2 x 4 H2O 0.180 g Na2B4O7 x 10 H2O 0.450 g ZnSO4 x 7 H2O 22.000 mg CuCl2 x 2 H2O 5.000 mg Na2MoO4 x 2 H2O 3.000 mg VOSO4 x 5 H2O 3.800 mg CoSO4 x 7 H2O 2.000 mg Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved
trace elements solution for DSMZ Medium 158
A trace elements solution comprised of ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, and cobalt sulfate.
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, fumaric acid, sodium formate, yeast extract, manganese chloride, ferrous sulfate, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium157.pdf 157. WOLINELLA SUCCINOGENES MEDIUM (NH4)2SO4 1.00 g K2HPO4 5.00 g Fumaric acid 3.00 g Na-formate 3.00 g Yeast extract 1.00 g MgCl2 x 6 H2O 0.20 g FeSO4 x 7 H2O 0.02 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine and sulfide), boil for 1 min, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in anoxic tubes or bottles and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under N2 and adjust pH of completed medium to 7.0 - 7.2. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium containing casitone, yeast extract, cellobiose (or filter paper) and calcium chloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium160.pdf 160. CYTOPHAGA HUTCHINSONII MEDIUM 1. To medium 67 add 0.5% cellobiose (filter-sterilized) or 2. Distribute medium 67 (without agar) in 5 ml amounts in test tubes, add a strip of filter paper (7 cm long) to each and autoclave, or place sterile pieces of filter paper on the surface of agarized medium 67. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium159.pdf 159. TREPONEMA BRYANTII MEDIUM Rumen fluid, clarified 300.000 ml CaCl2 x 2 H2O 0.120 g MgSO4 x 7 H2O 0.180 g KH2PO4 0.450 g K2HPO4 0.450 g NaCl 0.900 g (NH4)2SO4 0.900 g Resazurin 0.001 g Cysteine-HCl x H2O 1.000 g Distilled water 580.000 ml Adjust pH to 7.0 with KOH NaHCO3 solution (5% w/v) 100.000 ml Glucose solution (10% w/v) 20.000 ml The medium is prepared under 100% CO2 atmosphere. Sodium bicarbonate and glucose solution are filter-sterilized and added to the main part of the medium after it has been autoclaved and cooled to room temperature. Final pH should be 6.7 - 7.0. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of clarified rumen fluid, calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, ammonium sulfate, resazurin, cysteine hydrochloride, sodium bicarbonate, and glucose. Prepared under an atmosphere of carbon dioxide.
An organic-rich, liquid culture medium comprised of clarified rumen fluid, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, trace elements, ferrous sulfate, yeast extract, trypticase (trypticase peptone), fatty acid mixture, resazurin, vitamins, L-cysteine hydrochloride, and sodium sulfide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium161.pdf 161. METHANOMICROBIUM MOBILE MEDIUM Rumen fluid, clarified (see medium 1310) 300.00 ml K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 0.60 g MgSO4 x 7 H2O 0.13 g CaCl2 x 2 H2O 8.00 mg Trace elements (see medium 141) 10.00 ml FeSO4 x 7 H2O 2.00 mg Yeast extract (Difco) 1.00 g Trypticase (BBL) 1.00 g Fatty acid mixture (see medium 119) 20.00 ml Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 660.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium for 30 – 45 min with 80% H2 and 20% CO2 gas mixture to make it anoxic. Add and dissolve bicarbonate and adjust pH to 6.8, then autoclave under 80% H2 and 20% CO2 gas mixture. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. For incubation use 80% H2 and 20% CO2 gas mixture at two atmospheres of pressure. Adjust pH of final medium to 6.5. © 2014 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium163.pdf 163. DESULFOVIBRIO MEDIUM (MARINE) Solution A: NaCl 25.0 g K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with oxygen-free N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. For DSM 10520 adjust final pH of autoclaved medium to 7.5 using a sterile anoxic stock solution of NaHCO3 (10% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. © 2015 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium chloride, potassium phosphate, ammonium chloride, sodium sulfate, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen.
Similar to DSMZ Medium 163, except the final pH is reduced to 7.5 and sodium bicarbonate is added. Also prepared under an atmosphere of dinitrogen and carbon dioxide.
DSM 10520 is Desulfovibrio vietnamensis DSM 10520
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, sodium molybdate, methylamine hydrochloride, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium162.pdf 162. HYPHOMICROBIUM MEDIUM KH2PO4 1.36 g Na2HPO4 2.13 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 6.00 mg FeSO4 x 7 H2O 3.00 mg MnSO4 x H2O 1.00 mg Na2MoO4 x 2 H2O 1.50 mg Methylamine hydrochloride 3.38 g Yeast extract 0.10 g Distilled water 1000.00 ml Adjust pH to 7.4 with NaOH. Autoclave 20 min at 121˚C; final pH: 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ferrous sulfate, vitamins, trace elements, cellobiose (or cellulose), resazurin, sodium bicarbonate, cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium165.pdf 165. ACETIVIBRIO CELLULOLYTICUS MEDIUM Mineral solution 1 (see below) 75.000 ml Mineral solution 2 (see below) 75.000 ml FeSO4 x 7 H2O 0.020 g Vitamin solution (see medium 141) 10.000 ml Trace element solution (see medium 141) 10.000 ml Cellobiose or cellulose 3.000 g (MN 300, Whatman CF II, Kleenex tissue paper, or HCl treated cotton) Resazurin 0.001 g NaHCO3 2.000 g Cysteine-HCl x H2O 0.250 g Na2S x 9 H2O 0.250 g Distilled water 830.000 ml Adjust pH to 7.6. Gas atmosphere: 80% N2 + 20% CO2. Final pH after autoclaving is 7.2. Cellobiose is filter-sterilized separately, flushed and stored under nitrogen gas until needed. Cysteine and sodium sulfide are each autoclaved separately in concentrated solutions under nitrogen gas. Mineral solution 1: K2HPO4 3.900 g Distilled water 1000.000 ml Mineral solution 2: KH2PO4 2.400 g (NH4)2SO4 6.000 g NaCl 0.590 g MgSO4 x 7 H2O 1.200 g CaCl2 x 2 H2O 0.720 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of ammonium sulfate, magnesium sulfate, sodium phosphate, potassium phosphate, trace elements, methylamine hydrochloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium166.pdf 166. HYPHOMICROBIUM STRAIN X MEDIUM (NH4)2SO4 1.00 g MgSO4 x 7 H2O 0.20 g NaH2PO4 x H2O 0.50 g K2HPO4 1.55 g Trace element solution 0.20 ml (see medium 69) Methylamine hydrochloride 3.40 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.2. For liquid medium use only 0.7 g of methylamine hydrochloride per liter. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of glucose, yeast extract, calcium chloride, magnesium sulfate, cysteine hydrochloride, agar, sodium bicarbonate, and potassium phosphate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium169.pdf 169. SPIROCHAETA ZUELZERAE MEDIUM Solution A: Glucose 2.00 g Yeast extract (Difco) 4.00 g CaCl2 x 2 H2O 0.04 g MgSO4 x 7 H2O 0.50 g Cysteine-HCl x H2O 0.50 g Agar (Bacto, Difco) (if necessary) 10.00 g Distilled water 960.00 ml Adjust pH to 7.2 with KOH. Solution B: NaHCO3 1.00 g Distilled water 20.00 ml Solution C: 0.5 M K-phosphate buffer, pH 7.4 20.0 ml Solution A (without cysteine) is boiled for few minutes to remove dissolved oxygen, and cooled to room temperature under a stream of 100% nitrogen gas. Then cysteine is added and the pH adjusted to 7.2. The three parts are sterilized separately each by autoclaving under 100% nitrogen atmosphere and combined thereafter. Final pH of the complete medium is 7.5. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium170.pdf 170. YEAST EXTRACT - SKIM MILK MEDIUM Skim milk (Difco) 10.0 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of skim milk powder, yeast extract, and agar.
Skim milk powder rehydrated at a 10% solution is pH 6.3 and Bacto Yeast Extract in solution is pH 6.3-7.3. One cup contains 237 mL; 1 cup of skim milk contains 127 mg sodium and 407 mg potassium (=0.534g/237mL = 0.2% due to cations, assume 0.4% due to cations and anions).
A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium171.pdf 171. ACETOGENIUM MEDIUM K2HPO4 0.220 g KH2PO4 0.220 g NaH2PO4 x H2O 4.500 g Na2HPO4 x 12 H2O 6.100 g NH4Cl 0.310 g (NH4)2SO4 0.220 g NaCl 0.450 g MgSO4 x 7 H2O 0.090 g CaCl2 x 2 H2O 0.006 g FeSO4 x 7 H2O 0.002 g Trace element solution (see medium 141) 10.000 ml Resazurin 0.001 g L-Cysteine-HCl x H2O 0.500 g Na2S x 9 H2O 0.500 g Distilled water 1000.000 ml Adjust final pH to 6.5. Use 80% H2 + 20% CO2 mixture as gas atmosphere at one atmosphere overpressure. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium172.pdf 172. CYTOPHAGA (marine) MEDIUM Yeast extract (Difco) 1.0 g Tryptone (Difco) 1.0 g NaCl 24.7 g KCl 0.7 g MgSO4 x 7 H2O 6.3 g MgCl2 x 6 H2O 4.6 g CaCl2 x 2 H2O 1.2 g NaHCO3 0.2 g Agar (Difco) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. Sodium bicarbonate and calcium chloride are autoclaved separately, each in a small volume of distilled water. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, tryptone, sodium chloride, potassium chloride, magnesium sulfate, magnesium chloride, calcium chloride, sodium bicarbonate, and agar.
An inorganic salts solution comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride.
trace elements solution for DSMZ Medium 150a
A trace elements solution comprised of ferric trichloride, copper sulfate, boric acid, manganese sulfate, sodium molybdate, cobalt chloride, and zinc sulfate.
A defined organic solution containing the enzyme catalase, sterilized by passing it through a 0.2 µm filter.
An organic-rich, solid culture medium comprised of ammonium chloride, potassium phosphate, magnesium sulfate, calcium chloride, sodium chloride, ferric trichloride, yeast extract, agar, glucose, sodium bicarbonate, and sodium sulfide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium172a.pdf 172a. CY S2 MEDIUM NH4Cl 1.00 g KH2PO4 1.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.06 g NaCl 30.00 g FeCl3 x 6 H2O 1.25 mg Yeast extract 1.00 g Agar 1.00 g Distilled water 1000.00 ml Adjust pH to 7.0 and autoclave. After autoclaving, add from separate sterile solutions per liter medium: Glucose (10% w/v) 10.0 ml NaHCO3 (5% w/v) 10.0 ml Na2S x 9 H2O (3% w/v) 3.3 ml Aseptically fill sterile 10-ml screwcap tubes almost completely with the medium. Make stab inoculation. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), yeast extract, resazurin, sea water, tris buffer (or potassium phosphate), and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium173.pdf 173. SPIROCHAETA LITORALIS MEDIUM Glucose 2.0 g Trypticase (BBL) 1.0 g Yeast extract (Difco) 1.0 g Resazurin 1.0 mg Distilled water 200.0 ml Sea water 750.0 ml 1 M Tris-HCl buffer, pH 7.5 50.0 ml Cysteine-HCl x H2O 0.5 g Adjust pH to 7.5 prior to autoclaving. Add 1% agar (Difco) for stabs. Prereduced medium is prepared under 100% nitrogen atmosphere. The Tris-HCl buffer can be replaced by potassium phosphate buffer (1 M, pH 7.4) which is autoclaved separately. After adding to the main part of the medium some precipitate develops. Final pH is about 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich liquid culture medium comprised of glucose, yeast extract, peptone, resazurin, and thioglycolate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium174.pdf 174. SPIROCHAETA STENOSTREPTA MEDIUM Glucose 5.0 g Yeast extract (Difco) 2.0 g Peptone (Difco) 2.0 g Resazurin 1.0 mg Thioglycolate 0.5 g Distilled water 1000.0 ml Adjust pH to 7.6 with KOH before autoclaving. Add 1% agar for stabs. Prereduced medium is prepared under 100% nitrogen atmosphere. Strain is sensitive to phosphates! Pay close attention to clean glassware! © 2009 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium182.pdf 182. SULFOLOBUS MEDIUM Yeast extract (DIFCO) 1.00 g Casamino acids (DIFCO) 1.00 g KH2PO4 3.10 g (NH4)2SO4 2.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.25 g Trace element solution SL-10 (see medium 320) 10.00 ml Distilled water 1000.00 ml Adjust pH to 4.0 - 4.2 with 10 N H2SO4 at room temperature. © 2009 DSMZ GmbH - All rights reserved
An organic, liquid culture medium comprised of yeast extract, casamino acids, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium181.pdf 181. SELENOMONAS RUMINANTIUM MEDIUM Glucose 1.0 g Trypticase (BBL) 5.0 g KH2PO4 1.0 g Na-acetate 4.0 g Yeast extract 2.0 g n-Valeric acid 0.1 ml Resazurin 1.0 mg Na2CO3 4.0 g Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Adjust pH to 7.0. Gas atmosphere: 100% CO2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), potassium phosphate, sodium acetate, yeast extract, valeric acid, resazurin, sodium carbonate, and cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium183.pdf 183. TRICHOCOCCUS MEDIUM Tryptone (Difco) 10.0 g Glucose 3.0 g MgCl2 x 6 H2O 1.1 g Na2SO4 4.0 g KCl 0.7 g Distilled water 1000.0 ml Adjust pH to 7.3. Glucose is filter-sterilized. © 2007 DSMZ GmbH - All rights reserved
An organic, liquid culture medium comprised of tryptone, glucose, magnesium chloride, sodium sulfate, and potassium chloride.
An organic-rich, liquid culture medium comprised of yeast extract, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements (SL-10).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium182a.pdf 182a. SULFOLOBUS MT-4 MEDIUM Yeast extract (Difco) 2.00 g KH2PO4 3.10 g (NH4)2SO4 2.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.25 g Trace element solution (see medium 320) 10.00 ml Distilled water 1000.00 ml Adjust pH of the medium at room temperature to 3.5 with 10 N H2SO4 prior to autoclaving. © 2009 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium185.pdf 185. THERMOPROTEUS MEDIUM (NH4)2SO4 0.264 g FeSO4 x 7 H2O 0.556 g MgSO4 x 7 H2O 0.492 g CaSO4 x 2 H2O 0.344 g KH2PO4 0.014 g Resazurin 1.000 mg Trace elements solution (see below) 1.000 ml Yeast extract 0.200 g Soluble starch 5.000 g Sulfur, powdered 10.000 g Na2S x 9 H2O 0.500 g Distilled water 1000.000 ml Trace elements solution: NaF 840.0 mg MnCl2 x 4 H2O 180.0 mg Na2B4O7 x 10 H2O 450.0 mg ZnSO4 x 7 H2O 22.0 mg CuCl2 x 2 H2O 5.0 mg Na2MoO4 x 2 H2O 3.0 mg CoSO4 x 7 H2O 1.0 mg Distilled water 1000.0 ml Prepare medium (without starch, sodium sulfide, sulfur) anaerobically under N2 gas atmosphere. Adjust pH to 5.5 with H2SO4 before sterilization. Distribute the medium into tubes containing the appropriate amount of sulfur powder. Sterilize medium by heating for 3 h at 90 - 100˚C on three subsequent days. Before use, add to the medium starch and sodium sulfide. Medium pH is 5.5. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, ferrous sulfate, magnesium sulfate, calcium sulfate, potassium phosphate, resazurin, trace elements, yeast extract, soluble starch, elemental sulfur, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
trace elements solution for DSMZ Medium 185
A trace elements solution comprised of sodium fluoride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, and cobalt sulfate.
An organic, solid culture medium comprised of sucrose, malt extract, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium187.pdf 187. MEDIUM FOR OSMOPHILIC FUNGI (M 40 Y) Sucrose 400.0 g Malt extract 20.0 g Yeast extract 5.0 g Agar 20.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, malt extract, peptone from soybeans (soy peptone), glucose, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium186.pdf 186. UNIVERSAL MEDIUM FOR YEASTS (YM) Yeast extract 3.0 g Malt extract 3.0 g Peptone from soybeans 5.0 g Glucose 10.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of sodium nitrate, magnesium sulfate, potassium chloride, ferric sulfate, potassium phosphate, agar, yeast extract, and filter paper.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium188.pdf 188. CHAETOMIUM MEDIUM NaNO3 2.00 g MgSO4 x 7 H2O 0.50 g KCl 0.50 g Fe2(SO4)3 x H2O 0.01 g KH2PO4 0.14 g K2HPO4 1.20 g Agar 15.00 g Yeast extract 0.02 g Distilled water 1000.00 ml Adjust pH to 7.2. Place a strip of sterile filter paper on cooled agar and inoculate on filter strip. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, soluble starch, potassium phosphate, magnesium sulfate, and agar.
A solution of K2HPO4 will have a pH around 8.0.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium190.pdf 190. YpSs MEDIUM Yeast extract 4.0 g Soluble starch 15.0 g K2HPO4 1.0 g MgSO4 x 7 H2O 0.5 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of boiled oat flakes (boiled rolled oats) and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium189.pdf 189. OAT FLAKE MEDIUM Oat flakes 30.0 g Agar 15.0 g Distilled water 1000.0 ml Boil oat flakes in water for 10 min; fill up to 1 l and add agar. Autoclave 20 min at 121˚C. Shake before pouring. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of corn meal extract and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium191.pdf 191. CORN MEAL AGAR Blend 50.0 g corn meal in 800 ml of distilled water. Leave overnight in the refrigerator. Heat at 60˚C for one hour. Filter and make up volume to 1 l. Add 10.0 g agar and heat to dissolve, then autoclave. © 2007 DSMZ GmbH - All rights reserved
A organic-rich, solid culture medium comprised of yeast extract, sodium acetate, ammonium sulfate, magnesium sulfate, potassium phosphate, thiamine hydrochloride, hemin, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium192.pdf 192. PILOBOLUS MEDIUM Yeast extract 2.00 g Na-acetate 10.00 g (NH4)2SO4 0.66 g MgSO4 x 7 H2O 0.50 g K2HPO4 1.00 g Thiamine-HCl x 2 H2O 10.00 mg Haemin (dissolved in 10.00 mg 37.5 ml 0.1 N NaOH) Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 8.0. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193.pdf 193. DESULFOBACTER SP. MEDIUM I Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. DSM 1744, DSM 10631, and DSM 16697: Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 2034: Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml DSM 2059 and DSM 27197: Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml DSM 4660: Solution D: Phenol 0.10 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 6637: Supplement medium with 3.4 g/l KNO3 and omit solution F. Solution D: Acetone 0.58 g Distilled water 10.00 ml Add after autoclaving 5.0 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration. DSM 8775: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 10711: Solution D: Na-pyruvate 2.50 g Yeast extract 0.50 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 14956: Solution D: Methanol 1.60 g Distilled water 10.00 ml DSM 15102: Solution D: D-Glucose 3.50 g Distilled water 10.00 ml Add after autoclaving 1.0 g/l yeast extract from a sterile, anoxic stock solution. DSM 18843: Solution D: Na-stearate 0.62 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration. DSM 25524: Solution D: Na-octanoate 0.33 g Distilled water 10.00 ml Add after autoclaving 1 ml/l seven vitamins solution (see medium 503) from an anoxic stock solution sterilized by filtration © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potasium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 193, except sodium L-lactate and yeast extract are added.
DSMZ Medium 193.1 -< for DSM 1744, DSM 10631, and DSM 16697
DSM 1744 is Desulfovibrio vulgaris DSM 10631 is Desulfovibrio aespoeensis Aspo-2 DSM 16697 is Desulfomicrobium thermophilum DSM 16697
Similar to DSMZ Medium 193, except sodium acetate is added.
DSM 2034 is Desulfobacter postgatei 2ac9
Similar to DSMZ Medium 193, except phenol and seven vitamins solution are added.
DSM 4660 is Desulfatiglans anilini DSM 4660
Similar to DSMZ Medium 193, except potassium nitrate, acetate, and sodium pyruvate are added and sodium sulfide is omitted.
Similar to DSMZ Medium 193, except sodium pyruvate is added.
DSM 8775 is Desulfotomaculum sp. DSM 8775
DSM 10711 is Desulfovibrio inopinatus DSM 10711
Similar to DSMZ Medium 193, except sodium pyruvate, yeast extract, and seven vitamins solution are added.
Similar to DSMZ Medium 193, except methanol is added.
DSM 14956 is Desulfotomaculum solfataricum
Similar to DSMZ Medium 193, except glucose and yeast extract are added.
DSM 15102 is Garciella nitratireducens DSM 15102
Similar to DSMZ Medium 193, except sodium stearate and seven vitamins solution are added.
DSM 18843 is Desulfatiferula olefinivorans
Similar to DSMZ Medium 193, except sodium octanoate and seven vitamins solution are added.
DSM 2059 is Desulfococcus multivorans DSM 2059 DSM 27197 is Desulfatiglans parachlorophenolica
DSMZ Medium 193.3 -< for DSM 2059 and DSM 27197
Similar to DSMZ Medium 193, except sodium benzoate is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193b.pdf 193b. DESULFOFABA MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g KCl 0.50 g MgCl2 x 6 H2O 0.60 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 860.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 503) 1.00 ml Solution F: CaCl2 x 2 H2O 1.50 g MgCl2 x 6 H2O 11.50 g Distilled water 20.00 ml Solution G: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D, F and G are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to G are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 2075 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, potassium chloride, magnesium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, calcium chloride, magnesium chloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 193b, except sodium propionate is omitted and sodium butyrate, sodium caproate, and sodium octanoate are added.
DSM 2075 is Desulfarculus baarsii DSM 2075
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, poly-(R)-3-hydroxybutyric acid, resazurin, trace elements, sodium bicarbonate, sodium pyruvate, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium193a.pdf 193a. PHB/PYRUVATE MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 7.00 g MgCl2 x 6 H2O 1.30 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Poly[(R)-3-hydroxybutyric acid] (SIGMA) 1.00 g Resazurin 1.00 mg Distilled water 860.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-pyruvate 5.00 g Distilled water 20.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% H2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% H2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 6.8 - 7.0. After inoculation, pressurize vessels with 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium benzoate added.
DSM 28570 is Desulfoprunum benzoelyticum
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium194.pdf 194. DESULFOBULBUS SP. MEDIUM (FRESHWATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 50.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2055 Na-propionate is replaced by 0.70 g/l Na-butyrate, 0.30 g/l Na-caproate and 0.15 g/l Na-octanoate added after autoclaving from sterile anoxic stock solutions prepared under N2. Adjust final pH of medium to 7.7. For DSM 3852 Na-propionate is replaced by 0.70 g/l ethanol added after autoclaving from a sterile anoxic stock solution prepared under N2. In addition, solution A is supplemented with 0.10 g/l each of yeast extract and casamino acids. For DSM 5092 Na-propionate is replaced by 0.90 g/l putrescine added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5193 supplement medium with 0.50 g/l yeast extract and replace Napropionate with 0.90 g/l Na-methoxyacetate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5433 Na-propionate is replaced by 1.50 g/l 1,2-propanediol added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 5502 and DSM 5503 Na-propionate is replaced by 3.00 g/l ammonium oxalate monohydrate. In addition, solution A is supplemented with 1.00 g/l yeast extract. Adjust pH of final medium to 6.8 – 7.0. For DSM 5651 Na-propionate is replaced by 1.50 g/l of Na-(D/L)-3-hydroxybutyrate as substrate. For DSM 6283 Na-propionate is replaced by 2.50 g/l Na-(DL)-lactate and 1.00 g/l yeast extract added after autoclaving from sterile anoxic stock solutions prepared under N2. Final pH of the medium should be 6.8 - 7.0. For DSM 7474, DSM 7475, and DSM 7476 Na-propionate is replaced by 0.40 g/l 3,4,5- trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 10291 and DSM 24454 Na-propionate is replaced by 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.0 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration. For DSM 12016 Na-propionate is replaced by 0.10 ml/l propanol added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 g/l yeast extract. When growth has started, feed again same amount of propanol. For DSM 13527 and DSM 21556 Na-propionate is replaced by 1.00 g/l Na-butyrate added after autoclaving from a sterile anoxic stock solution prepared under N2. For DSM 14880 Na-propionate is replaced by 0.50 g/l yeast extract and 2.20 g/l sodium pyruvate added after autoclaving from anoxic stock solutions sterilized by filtration. For DSM 15970 Na-propionate is replaced by 0.60 g/l syringic acid (neutralized with NaOH) added after autoclaving from a sterile, anoxic stock solution prepared under N2. Prior to inoculation the completed medium should equilibrate over night. For DSM 28570 Na-propionate is replaced by 0.15 g/l Na-benzoate added after autoclaving from a sterile, anoxic stock solution prepared under N2. © 2015 DSMZ GmbH - All rights reserved
DSM 15970 is Parasporobacterium paucivorans DSM 15970
Similar to DSMZ Medium 194, except sodium propionate is omitted and syringic acid and sodium hydroxide are added.
DSM 14880 is Desulfotomaculum nigrificans CO-1-SRB
Similar to DSMZ Medium 194, except sodium propionate is omitted and yeast extract and sodium pyruvate are added.
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium butyrate is added.
DSM 13527 is Desulforegula conservatrix Mb1Pa DSM 21556 is Desulfovibrio butyratiphilus
DSMZ Medium 194.12 -< for DSM 13527 and DSM 21556
Similar to DSMZ Medium 194, except sodium propionate is omitted and propanol and yeast extract are added.
DSM 10291 is Desulfovibrio sp. DSM 24454 is Desulfovibrio cf. magneticus IFRC170
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium lactate and seven vitamins solution are added.
DSMZ Medium 194.10 -< for DSM 10291 and DSM 24454
DSM 7474 is Desulfotomaculum sp. DSM 7475 is Desulfotomaculum sp. DSM 7476 is Desulfotomaculum sp.
DSMZ Medium 194.9 -< for DSM 7474, DSM 7475, and DSM 7476
Similar to DSMZ Medium 194, except sodium propionate is omitted and 3,4,5-trimethoxybenzoic acid and seven vitamins solution are added.
DSM 6283 is Anaeroarcus burkinensis DSM 6283
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium lactate and yeast extract are added. The pH is reduced slightly to 6.8-7.0.
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium 3-hydroxybutyrate is added.
DSM 5502 is Oxalobacter vibrioformis DSM 5503 is Oxalophagus oxalicus
Similar to DSMZ Medium 194, except sodium propionate is omitted and ammonium oxalate and yeast extract are added. The pH is reduced slightly to 6.8-7.0.
DSMZ Medium 194.6 -< for DSM 5502 and DSM 5503
DSM 5433 is Desulfovibrio alcoholivorans DSM 5433
Similar to DSMZ Medium 194, except sodium propionate is omitted and 1,2-propanediol (propane-1,2-diol) is added.
Similar to DSMZ Medium 194, except sodium propionate is omitted and yeast extract and sodium methoxyacetate are added.
DSM 5092 is Anaerovorax odorimutans DSM 5092
Similar to DSMZ Medium 194, except sodium propionate is omitted and putrescine is added.
Similar to DSMZ Medium 194, except sodium propionate is omitted, and ethanol, yeast extract, and casamino acids are added.
DSM 2055 is Desulfobotulus sapovorans DSM 2055
Similar to DSMZ Medium 194, except sodium propionate is omitted and sodium butyrate, sodium caproate, and sodium octanoate are added. The pH is increased to 7.7.
A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195.pdf 195. DESULFOBACTER SP. MEDIUM (ACETATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-acetate x 3 H2O 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195a.pdf 195a. DESULFOBACTER SP. MEDIUM (FUMARATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na2-fumarate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate solution, resazurin, trace elements, sodium bicarbonate, sodium fumarate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195b.pdf 195b. DESULFOBULBUS SP. MEDIUM (MARINE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-propionate 1.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium propionate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of carbon dioxide and dinitrogen.
Similar to DSMZ Medium 195c, except casamino acids and trypticase pepetone are added.
DSM 16109 is Desulfovibrio alaskensis DSM 16109 DSM 17464 is Desulfovibrio alaskensis G20
DSMZ Medium 195c.5 -< for DSM 16109 and DSM 17464
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium195c.pdf 195c. DESULFOBACTER SP. MEDIUM (LACTATE) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-L-lactate 2.50 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. For DSM 9120, DSM 10141, DSM 17456 and DSM 19275 supplement medium with 1 g/l yeast extract added to the autoclaved medium from a sterile anoxic stock solution. For DSM 11974 supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 14278 supplement medium with 0.5 g/l Na-acetate added to the autoclaved medium from a sterile anoxic stock solution. For DSM 14982 supplement medium with 5.0 g/l Na2S2O3 x 5 H2O added to the autoclaved medium from an anoxic stock solution sterilized by filtration. For DSM 16109 and DSM 17464 supplement medium with 2.0 g/l Casamino acids (BACTO) and 2.0 g/l Trypticase peptone (BD BBL) added to the autoclaved medium from sterile anoxic stock solutions. © 2014 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 195c, except sodium thiosulfate is added.
DSM 14278 is not in www.dsmz.de, Tax Browser, or StrainInfo.
Similar to DSMZ Medium 195c, except sodium acetate is added.
Similar to DSMZ Medium 195c, except seven vitamins solution is added.
DSM 11974 is Desulfovibrio zosterae DSM 11974
DSM 9120 is Desulfobacterium zeppelinii DSM 10141 is Desulfovibrio acrylicus DSM 10141 DSM 17456 is Desulfovibrio marinisediminis DSM 17456 DSM 19275 is Desulfovibrio tunisiensis DSM 19275
DSMZ Medium 195c.1 -< for DSM 9120, DSM 10141, DSM 17456 and DSM 19275
Similar to DSMZ Medium 195c, except yeast extract is added.
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium lactate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium202.pdf 202. DESULFONEMA MAGNUM MEDIUM Solution A: NaCl 25.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 6.80 g CaCl2 x 2 H2O 1.40 g KCl 0.72 g KH2PO4 0.14 g NH4Cl 0.25 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 1000.00 ml Solution B: Na-benzoate 0.60 g Distilled water 10.00 ml Solution C: Vitamin solution (see medium 141) 10.00 ml Solution D: KAl(SO4)2 x 12 H2O 0.48 g Distilled water 10.00 ml Solution E: Na2CO3 1.00 g Distilled water 20.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Solution A is boiled for a 1 min, cooled to room temperature, while gassing with 80% N2 and 20% CO2 gas mixture to reach a pH of around 6, then distributed anoxically in cultivation vessels and autoclaved under the same gas atmosphere. Solutions B, D, and F are autoclaved separately under 100% N2 gas, solution E is flushed with 80% N2 and 20% CO2 gas mixture to remove dissolved oxygen and autoclaved, solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions B to F are added to the autoclaved solution A in the sequence as indicated. Final pH of the medium should be 7.0. After completion the medium should equilibrate overnight and a white precipitate should be apparent. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) just before inoculation may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2010 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, potassium phosphate, ammonium chloride, trace elements, selenite-tungstate, resazurin, sodium benzoate, vitamins, potassium aluminum sulfate, sodium carbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 198, except sodium benzoate is omitted and sodium acetate is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium198.pdf 198. DESULFOSARCINA MEDIUM (BRACKISH WATER) Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 13.50 g MgCl2 x 6 H2O 2.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 870.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 5.00 g Distilled water 100.00 ml Solution D: Na-benzoate 0.60 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is gassed with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then autoclaved anoxically under the same gas mixture. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to F are added to the sterile, cooled solution A in the sequence as indicated. The complete medium is distributed anoxically under 80% N2 and 20% CO2 gas atmosphere into appropriate vessels. Final pH of the medium should be 7.1 - 7.4. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. For DSM 2650 Na-benzoate is replaced by 1.50 g/l Na-pyruvate and 1.00 g/l Na2-malate added after autoclaving from sterile anoxic stock solutions prepared under N2. For DSM 9990 Na-benzoate is replaced by 6.20 g/l Na-acetate. © 2015 DSMZ GmbH - All rights reserved
DSM 9990 is Thermodesulforhabdus norvegica
Similar to DSMZ Medium 198, except sodium benzoate is omitted and sodium pyruvate and sodium malate are added.
A minerals-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium benzoate, vitamins, sodium sulfide, and sodium dithionite. Prepared under an atmosphere of dinitrogen and carbon dioxide.
Similar to DSMZ Medium 203, except the yeast extract, trypticase peptone, and vitamins are omitted.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium203.pdf 203. METHANOTHERMUS FERVIDUS MEDIUM Mineral solution 1 (see below) 37.5 ml Mineral solution 2 (see below) 37.5 ml Ni(NH4)2(SO4)2 2.0 mg or NiCl2 x 6 H2O 1.0 mg FeSO4 x 7 H2O 2.0 mg Yeast extract 2.0 g Trypticase (BBL) 2.0 g Trace element solution (see medium 141) 10.0 ml Na2SO4 3.4 g Resazurin 1.0 mg NaHCO3 2.0 g Vitamin solution (see medium 141) 10.0 ml Na2S x 9 H2O 0.5 g L-Cysteine-HCl x H2O 0.5 g Distilled water 920.0 ml Mineral solution 1: K2HPO4 6.0 g Distilled water 1000.0 ml Mineral solution 2: KH2PO4 6.0 g (NH4)2SO4 6.0 g NaCl 12.0 g MgSO4 x 7 H2O 2.4 g CaCl2 x 2 H2O 1.6 g Distilled water 1000.0 ml Dissolve ingredients except bicarbonate, sulfide and vitamins, sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 – 45 min to make it anoxic, then add bicarbonate and adjust pH to 6.5. Add sodium sulfide and distribute medium under 80% N2 and 20% CO2 gas atmosphere into soda lime glass serum bottles (20 ml of medium per 100 ml bottle). Pressurize to 2 bar 80% H2 and 20% CO2 overpressure, then autoclave! Prior to inoculation add vitamins from a sterile anoxic stock solution sterilized by filtration and adjust pH of completed medium to 6.5. For DSM 3496 omit yeast extract, trypticase and vitamins. Alternative procedure: Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide, then adjust pH to 6.0 with sulfuric acid. Bring medium to the boil, then cool to room temperature while gassing with 80% H2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in glass serum bottles, seal, and autoclave without gas overpressure. Thereafter, add by injection vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and hydrogencarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 6.5. After inoculation pressurize bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2012 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, ammonium nickel sulfate (or ammonium chloride), ferrous sulfate, yeast extract, trypticase (trypticase peptone), trace elements, sodium sulfate, resazurin, sodium bicarbonte, vitamins, sodium sulfide, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen, carbon dioxide, and dihydrogen.
An organic-rich, solid culture medium comprised of sodium nitrate, potassium phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, zinc sulfate, agar, and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium204.pdf 204. ASPERGILLUS NIDULANS MINIMAL MEDIUM Solution A: NaNO3 6.00 g KH2PO4 1.52 g KCl 1.52 g Distilled water 500.00 ml Adjust pH to 6.5 with 2 N NaOH. Solution B: MgSO4 x 7 H2O 0.52 g FeSO4 x 7 H2O trace - ZnSO4 x 7 H2O trace - Agar 15.00 g Distilled water 250.00 ml Solution C: Glucose 10.00 g Distilled water 200.00 ml Autoclave separately for 15 min. Mix A, B and C before pouring plates. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium205.pdf 205. NATRONOBACTERIUM MEDIUM Casamino acids 15.0 g Na3-citrate x 2 H2O 3.0 g Glutamic acid 2.5 g MgSO4 x 7 H2O 2.5 g KCl 2.0 g NaCl 250.0 g Agar 20.0 g Add distilled water to give a final volume of 1000.0 ml. Dissolve agar by heating before adding sodium chloride. Adjust pH to 7.0 before autoclaving. Adjust pH to 8.5 with sterile 5% Na2CO3 after heat sterilization of the medium. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of casamino acids, sodium citrate, glutamic acid, magnesium sulfate, potassium chloride, sodium chloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium206.pdf 206. THERMODESULFOBACTERIUM MEDIUM Na2SO4 3.0 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.0 g Trace element solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Vitamin solution (see medium 141) 5.0 ml Resazurin 1.0 mg Yeast extract 1.0 g Na-L-lactate 4.0 g Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except yeast extract, lactate and sulfide) and sparge medium with 100% N2 gas to make it anoxic (c. 30 – 45 min). After sterilization add yeast extract, lactate, and sulfide from stock solutions prepared under N2 gas and autoclaved separately. Before use, neutralize the sodium sulfide solution by dropwise addition of 1 N HCl. Adjust pH of final medium to 6.8 - 7.0. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium sulfate, ammonium chloride, magnesium chloride, potassium phosphate, sodium phosphate, trace elements, ferrous sulfate, vitamins, resazurin, yeast extract, sodium lactate, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, trace elements, ferrous sulfate, vitamins, resazurin, sodium bicarbonate, yeast extract, sodium lactate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium207.pdf 207. PROPIONISPIRA MEDIUM KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.1 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g Trace element solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Vitamin solution (see medium 141) 5.0 ml Resazurin 1.0 mg NaHCO3 4.0 g Yeast extract 2.0 g Na-lactate 5.0 g Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Adjust pH to 7.2. Gas atmosphere: 80% N2 + 20% CO2. Prepare a 5% (w/v) solution of sodium sulfide anoxically under nitrogen and autoclave separately. Before use, neutralize by dropwise addition of 1 N HCl. © 2013 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 78, except arginine is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium209.pdf 209. EUBACTERIUM LENTUM MEDIUM To medium 78 add 0.5% (w/v) arginine. © 2007 DSMZ GmbH - All rights reserved
Wikipedia:Oat Flaking This process uses two large smooth or corrugated rolls spinning at the same speed in opposite directions at a controlled distance. Oat flakes, also known as rolled oats, have many different sizes, thicknesses and other characteristics depending on the size of oat groats passed between the rolls. Typically, the three sizes of steel cut oats are used to make instant, baby and quick rolled oats, whereas whole oat groats are used to make regular, medium and thick rolled oats. Oat flakes range in thickness from 0.36 mm to 1.00 mm.
Rolled oats that have been boiled (cooked in water).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium320.pdf 320. CLOSTRIDIUM CELLULOVORANS MEDIUM K2HPO4 x 3 H2O 1.00 g NH4Cl 1.00 g KCl 0.50 g MgSO4 x 7 H2O 0.50 g Trypticase peptone 0.50 g Yeast extract 0.50 g Rumen fluid, clarified or sludge fluid (see medium 119) 20.00 ml Trace element solution SL-10 (see below) 1.00 ml Resazurin 1.00 mg L-Cysteine-HCl x H2O 0.15 g Na2CO3 1.00 g Cellobiose 5.00 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except cysteine, carbonate, cellobiose and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture and add cysteine. Dispense under same gas atmosphere in culture vessels and autoclave. Add sulfide from a sterile anoxic stock solution prepared under N2 and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2. Sterilize cellobiose separately by filtration under N2 gas. Adjust pH of the completed medium to 7.0. Some strains can be adapted to cellulose as substrate using 10.0 g/l cellulose MN 301 (MACHEREY-NAGEL). 'Trace element solution SL-10: HCl (25%; 7.7 M) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. © 2012 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, potassium chloride, magnesium sulfate, trypticase peptone, yeast extract, clarified rumen fluid or sludge fluid, trace elements, resazurin, L-cysteine hydrochloride, sodium carbonate, cellobiose, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
An undefined organic chemical mixture comprised of the seed of corn (Zea mays), which has been ground, hydrated in water, and then heated. Finally, the liquid is filtered.
Thermoanaerobacterium thermosulfurogenes medium
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium211.pdf 211. THERMOANAEROBACTERIUM THERMOSULFUROGENES MEDIUM KH2PO4 0.3 g Na2HPO4 x 12 H2O 5.3 g NH4Cl 1.0 g MgCl2 x 6 H2O 0.2 g Trace elements solution (see medium 144) 10.0 ml FeSO4 x 7 H2O 1.5 mg Yeast extract 1.0 g Resazurin 0.5 mg D-Glucose 5.0 g Vitamin solution (see medium 141) 5.0 ml Na2S x 9 H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except glucose, vitamins and sulfide), adjust pH to 6.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add glucose and sulfide from anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized anoxic stock solution prepared under N2. Adjust pH of final medium to 6.0 – 6.5. For DSM 8683, DSM 8684 and DSM 16487 adjust pH of completed medium to 5.5. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, trace elements, ferrous sulfate, yeast extract, resazurin, D-glucose, vitamin solution, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
DSM 8683 is Thermoanaerobacterium sp. DSM 8684 is Thermoanaerobacterium sp. DSM 16487 is Thermoanaerobacterium aciditolerans
Similar to DSMZ Medium 211, except the pH is reduced to 5.5.
DSMZ Medium 211.1 -< for DSM 8683, DSM 8684 and DSM 16487
An organic-rich, solid culture medium comprised of nutrient broth, peptone, potassium phosphate, sodium chloride, glucose, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium237.pdf 237. ENB MEDIUM Nutrient Broth 8.0 g Peptone 5.0 g KH2PO4 1.5 g K2HPO4 3.5 g NaCl 5.0 g Glucose 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 6.8 - 7.0. Sterilize glucose separately. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, resazurin, sodium bicarbonate, vitamins, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium233.pdf 233. METHANOLOBUS TINDARIUS MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Resazurin 1.00 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml Methanol 5.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, methanol, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 6.5, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.5. © 2014 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium232b.pdf 232b. WEISSELA KOREENSIS MEDIUM Use medium 232 + 4 ml Resazurin solution (25 mg/100 ml). Cystein are added after the medium has been boiled and cooled und CO2. Distribute under N2 and autoclave. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 232, except resazurine is added. Prepared under an atmosphere of carbon dioxide and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium231.pdf 231. PEDIOCOCCUS DAMNOSUS MEDIUM Add 0.05% cysteine-hydrochloride to medium 11 and adjust to pH 5.2. Medium should be filled up into crew capped tubes (Wheaton). © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 11, except cysteine hydrochloride is added and the pH is reduced to 5.2.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium232.pdf 232. MRS MEDIUM WITH CYSTEINE Casein peptone, tryptic digest 10.00 g Meat extract 10.00 g Yeast extract 5.00 g Glucose 20.00 g Tween 80 1.00 g K2HPO4 2.00 g Na-acetate 5.00 g (NH4)2 citrate 2.00 g MgSO4 x 7 H2O 0.20 g MnSO4 x H2O 0.05 g Distilled water 1000.00 ml Adjust pH to 6.2 - 6.5. Add 0.05% cysteine-hydrochloride. © 2012 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of casein peptone, tryptic digest (casitone), meat extract, yeast extract, glucose, Tween 80 (polysorbate 80), potassium phosphate, sodium acetate, ammonium citrate, magnesium sulfate, manganese sulfate, and cysteine hydrochloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium227.pdf 227. PEDIOCOCCUS HALOPHILUS MEDIUM To medium 11 add 6.5% NaCl. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 11, except sodium chloride is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium225.pdf 225. SOUR DOUGH MEDIUM Maltose 20.0 g Yeast extract 3.0 g Fresh yeast extract 15.0 ml Tween 80 0.3 g Casein peptone, tryptic digest 6.0 g Distilled water up to 1000.0 ml Adjust pH to 5.6 with 20% lactic acid or HCl. Fresh yeast extract is prepared by autoclaving a 20% suspension of commercial baker's yeast in distilled water for 30 minutes at 121˚C, allowing the suspension to settle overnight at 2 to 8˚C, decanting and further clarifying the supernatant by centrifugation. The extract prepared in this manner contains 1.5% solids and if not to be used within a few days, should be frozen or freeze-dried immediately. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of maltose, yeast extract, autoclaved Baker's yeast, tween 80 (polysorbate 80), casein peptone, tryptic digest (casitone), lactic acid or hydrochloric acid.
An organic-rich, liquid culture medium comprised of casamino acids, yeast extract, glucose, ammonium citrate, potassium phosphate, magnesium sulfate, ferric chloride, and acetylacetone.
226. AUREOBACTERIUM TERREGENS MEDIUM Casamino acids 2.0 g Yeast extract 1.0 g Glucose 1.0 g (NH4) citrate 1.0 g K2HPO4 2.0 g MgSO4 x 7 H2O 0.5 g FeCl3 x 6 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 7.0. After sterilization, aseptically add 1 ml/l of a 10% solution of acetylacetone in ethanol. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium222.pdf 222. SP - MEDIUM Raffinose 1.00 g Sucrose 1.00 g Galactose 1.00 g Soluble starch 5.00 g Casitone 2.50 g MgSO4 x 7 H2O 0.50 g K2HPO4 0.25 g Agar (Difco) 15.00 g Distilled water 1000.00 ml Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of raffinose, sucrose, galactose, soluble starch, casitone, magnesium sulfate, potassium phosphate, and agar.
An organic-rich, liquid culture medium comprised of yeast extract, potassium phosphate, ferrous sulfate, sodium molybdate, manganese sulfate, magnesium sulfate, sodium chloride, calcium chloride, ammonium sulfate, biotin, glucose and sodium malate (disodium malate).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium221.pdf 221. AZOSPIRILLUM MEDIUM Yeast extract 0.05 g K2HPO4 0.25 g FeSO4 x 7 H2O 0.01 g Na2MoO4 x 2 H2O 1.00 mg MnSO4 x H2O 2.00 mg MgSO4 x 7 H2O 0.20 g NaCl 0.10 g CaCl2 x 2 H2O 0.02 g (NH4)2SO4 1.00 g Biotin 0.10 mg Distilled water 950.00 ml Adjust pH of the medium to 7.1. Add 1.5% agar if needed. Autoclave for 15 min at 121˚C. After sterilization add 25 ml each of filter-sterilized 20% glucose and 20% Na-malate. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium219.pdf 219. MYCOBACTERIUM MEDIUM Yeast extract 2.0 g Proteose peptone no. 3 2.0 g Casein peptone, tryptic digested 2.0 g Na2HPO4 x 12 H2O 2.5 g KH2PO4 1.0 g Na-citrate 1.5 g MgSO4 x 7 H2O 0.6 g Glycerol 50.0 ml Tween 0.5 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, proteose peptone, casein peptone tyrptic digest (casitone), potassium phosphate, sodium phosphate, sodium citrate, magnesium sulfate, glycerol, tween, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium218.pdf 218. BHI/3 MEDIUM To medium 215 add 10 g casein hydrolysate and 1 g starch. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of brain heart infusion, casein hydrolysate, and starch.
An organic-rich, liquid culture medium comprised of brain heart infusion and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium216.pdf 216. BHI/1 MEDIUM To medium 215 add 2.0 g yeast extract. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of brain heart infusion, casein hydrolysate, glucose, and yeast extract.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium217.pdf 217. BHI/2 MEDIUM To medium 215 add 10.0 g casein hydrolysate, 5.0 g glucose and 5.0 g yeast extract. © 2007 DSMZ GmbH - All rights reserved
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215b.pdf 215b. BHI MEDIUM WITH ADDITIONAL GLUCOSE Brain Heart Infusion (Difco) 37.0 g Glucose 5.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of brain heart infusion and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215c.pdf 215c. BHI MEDIUM FOR STRICT ANAEROBES Brain Heart Infusion (DIFCO) 37.00 g L-Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. For DSM 10643 supplement medium with 10.00 ml/l glycerol (87% w/v solution) from a sterile anoxic stock. For DSM 15692 prepare medium under 80% N2 and 20% CO2 gas atmosphere. Adjust final pH of the medium to 7.2 with a sterile anoxic solution of 10% (w/v) NaHCO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 19851 supplement medium with 10.0 ml/l haemin and 0.2 ml/l vitamin K1 solution (see medium 104). © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of brain heart infusion, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
Similar to DSMZ Medium 215c, except hemin and vitamin K solution are added.
starch that is soluble (having the physical quality of being dissolved) in water.
Charcoal that has been activated by a heating process or a chemical digestion process.
From: Schaedler Media (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Schaedler Agar is a base for several media formulations used for the recovery of anaerobic microorganisms. Principles of the Procedure The combination of three peptones derived from both animal and vegetable sources, dextrose and yeast extract render the basic formulation highly nutritious by providing nitrogenous growth factors, carbohydrates as energy sources and vitamins. The sheep blood and hemin also are important in stimulating the growth of fastidious microorganisms. As discussed above, the vitamin K1 additive is crucial for the recovery of certain anaerobes. The addition of the antimicrobial agents kanamycin and vancomycin in the agar medium renders the medium selective for gram-negative microorganisms. The kanamycin inhibits protein synthesis in susceptible organisms, whereas the vanco-mycin inhibits gram-positive bacteria by interfering with cell wall synthesis.8 Using Schaedler media, fastidious aerobes and anaerobes grow well; however, the type of organisms recovered is dependent on the environment utilized in the incubation process (aerobic, aerobic supplemented with carbon dioxide or anaerobic conditions). Formulae BBL™ Schaedler Agar Approximate Formula* Per Liter Pancreatic Digest of Casein.......................................... 8.2 g Peptic Digest of Animal Tissue...................................... 2.5 g Papaic Digest of Soybean Meal..................................... 1.0 g Dextrose...................................................................... 5.8 g Yeast Extract................................................................ 5.0 g Sodium Chloride.......................................................... 1.7 g Dipotassium Phosphate................................................ 0.8 g L-Cystine...................................................................... 0.4 g Hemin.......................................................................... 0.01 g Tris (hydroxymethyl) aminomethane............................. 3.0 g Agar.......................................................................... 13.5 g BBL™ Schaedler Broth Consists of the same ingredients without the agar. *Adjusted and/or supplemented as required to meet performance criteria. pH 7.6 ± 0.2
An organic-rich, liquid microbiological culture medium containing peptones, dextrose, cystine, and hemin. Used for the cultivation of anaerobic heterotrophic microorganisms.
Assays for the ability of a microorganism to assimilate L-lactate (L-lactic acid, (S)-lactate, (S)-lactic acid) as a sole source of carbon. Assimilation of lactate results in an increase in pH (alkalinization) as a result of decarboxylation of lactate into acetate and CO2 via lactate 2-monooxygenase.
An assay for the ability of a microorganism to metabolize the constituents of milk.
A chemical solution comprised of a mixture of isoamyl alcohol, para-dimethylaminobenzaldehyde, and hydrochloric acid. Used in the indole test assay.
Wikipedia: Kovac's reagent Kovac's reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde and concentrated hydrochloric acid. It is used for the diagnostical indole test, to determine the ability of the organism to split indole from the amino acid tryptophan. The indole produced yields a red complex with para-dimethylaminobenzaldehyde under the given conditions.[1]
not in Chebi or in any other ontologies- Jan 2018
2-nitrophenyl beta-D-galactopyranoside-6-phosphate-7
not in Chebi - Jan 2018 - added to request list
Similar to DSMZ Medium 215c, except prepared under an atmosphere of dinitrogen and carbon dioxide. pH is increased to 7.2 with sodium bicarbonate.
DSM 15692 is Atopostipes suicloacalis DSM 15692
Similar to DSMZ Medium 215c, except glycerol is added.
An organic-rich, liquid culture medium comprised of brain heart infusion and erythromycin.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215a.pdf 215a. BHI MEDIUM (modified) Brain heart infusion 37.0 g Erythromycin 10.0 mg Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 215
An organic-rich, liquid culture medium comprised of brain heart infusion in water.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium215.pdf 215. BHI MEDIUM Brain Heart Infusion (Difco) 37.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 214
Similar to DSMZ Medium 65, except starch is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium214.pdf 214. GYM+S MEDIUM To medium 65 add 20 g starch. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 212
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium212.pdf 212. SYNTHROPHOMONAS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Na2SO4 2.80 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). Mineral solution: KH2PO4 10.00 g MgCl2 x 6 H2O 6.60 g NaCl 8.00 g NH4Cl 8.00 g CaCl2 x 2 H2O 1.00 g Distilled water 1000.00 ml For DSM 2612A replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2805 replace butyric acid by 1.50 g/l Na-propionate and supplement medium with 1.00 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. For DSM 16706 replace butyric acid by 1.50 g/l Na-propionate. For DSM 26217 omit rumen fluid and butyric acid from solution A and add to the completed medium 7.00 g/l sucrose from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase (trypticase peptone), butryic acid, sodium sulfate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 212.1
Similar to DSMZ Medium 212, except butyric acid is omitted and sodium benzoate is added.
DSMZ Medium 212.2
Similar to DSMZ Medium 212, except butyric acid is omitted and sodium proptionate and glucose are added.
DSMZ Medium 212.3
Similar to DSMZ Medium 212, except butyric acid is omitted and sodium propionate is added.
DSM 16706 is Syntrophobacter sulfatireducens
DSMZ Medium 212.4
Similar to DSMZ Medium 212, except clarified rumen fluid and butyric acid are omitted, and sucrose is added.
Similar to Czapek Dox agar, except yeast extract, casamino acids, and tryptophan are added.
DSM strains: 44065 Saccharopolyspora hordei 44575 Saccharopolyspora thermophila 43368 Thermoactinomyces vulgaris 43369 Thermoactinomyces vulgaris 43796 Thermoactinomyces vulgaris 43795 Thermobifida alba 43793 Thermobifida fusca 43028 Thermocrispum municipale 43183 Thermomonospora curvata DSM 43183 17572 Tuberibacillus calidus DSM 17572
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium550.pdf 550. CYC-MEDIUM (modified following Cross and Attwell,1973) Czapek Dox agar (Merck) 48.00 g Yeast extract (Oxoid) 2.00 g Casamino acids (Difco) 6.10 g Tryptophan 0.02 g Distilled water 1000.00 ml pH 7.2 © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 213
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase (trypticase peptone), butyric acid, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium213.pdf 213. SYNTHROPHOMONAS MEDIUM (SULFATE FREE) Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Butyric acid 1.70 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution E: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 minutes. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D and E are autoclaved under 100% N2 gas. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Note: For agar stabs add 3.00 g/l agar to the completed medium from a sterile anoxic stock solution (2% w/v). For DSM 2612B replace butyric acid by 2.00 g/l Na-benzoate. For DSM 2909, DSM 2984, and DSM 21899 replace butyric acid by 1.50 g/l acetoin. For DSM 15682 and DSM 16215 replace butyric acid by 1.70 g/l crotonic acid. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 213.1
Similar to DSMZ Medium 213, except butyric acid is omitted and sodium benzoate is added.
DSMZ Medium 213.2
DSM 2909 is Geobacter sp. DSM 2984 is Pelobacter sp. DSM 21899 is Halobacterium salinarum
Similar to DSMZ Medium 213, except butyric acid is omitted and acetoin added.
DSMZ Medium 213.2 -< for DSM 2909, DSM 2984, and DSM 21899
DSMZ Medium 213.3
DSMZ Medium 213.3 -< for DSM 15682 and DSM 16215
Similar to DSMZ Medium 213, except butyric acid is omitted and crotonic acid is added.
DSM 15682 is Syntrophomonas curvata DSM 16215 is Syntrophomonas erecta
DSMZ Medium 210
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, potassium chloride, yeast extract, trypticase (trypticase peptone), vitamin solution, trace elements, thioglycolate, ascorbate, glucose, and sodium hydroxide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium210.pdf 210. HALOANAEROBIUM MEDIUM NaCl 130.0 g MgSO4 x 7 H2O 8.8 g KCl 1.0 g Yeast extract 10.0 g Trypticase (BBL) 10.0 g Vitamin solution (see medium 141) 10.0 ml Trace element solutionsee medium 144) 10.0 ml Distilled water 1000.0 ml Prepare the medium anaerobically under 100% N2. After autoclaving add from sterile solutions: Thioglycolate-ascorbate reducing agent (see below) 25.0 ml Glucose 10% (w/v) 25.0 ml 2 N NaOH 10.0 ml Final pH: 9.0. Thioglycolate-ascorbate solution: Na-thioglycolate 0.5 g Na-ascorbate 0.5 g Distilled water 25.0 ml Filter sterilize. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 230
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium230.pdf 230. TRYPTONE YEAST EXTRACT MINERAL MEDIUM Tryptone 10.0 g Yeast extract 10.0 g K2HPO4 0.5 g KH2PO4 0.5 g NaCl 1.0 g MgSO4 0.1 g CaCl2 0.1 g NaHCO3 6.0 g Resazurin 0.1 mg Cysteine-HCl x H2O 0.3 g Arginine 3.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, sodium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, resazurin, cyteine hydrochloride, and arginine.
mineral solution for DSMZ Medium 212
An inorganic salts solution comprised of potassium phosphate, magnesium chloride, sodium chloride, ammonium chloride, and calcium chloride.
DSMZ Medium 243
An organic-rich, solid culture medium comprised of peptone, yeast extract, agar, and filtered aged seawater or artificial sea water.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium243.pdf 243. SEAWATER YEAST PEPTONE AGAR Peptone 5.0 g Yeast extract 3.0 g Agar 12.0 g Distilled water 250.0 ml Filtered, aged seawater 750.0 ml (see below) Adjust pH to 7.8. Boil for 5 min, filter and readjust the pH to 7.3. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 246
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium246.pdf 246. SEA WATER AGAR Beef extract 10.0 g Peptone 10.0 g Agar 20.0 g Tap water 250.0 ml Sea water* 750.0 ml Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121˚C for 20 min. Directly after autoclaving, add warm (55˚C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. Artificial sea water: NaCl 28.13 g KCl 0.77 g CaCl2 x 2 H2O 1.60 g MgCl2 x 6 H2O 4.80 g NaHCO3 0.11 g MgSO4 x 7 H2O 3.50 g Distilled water © 2008 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of beef extract, peptone, agar, and filtered aged sea water or artificial sea water.
DSMZ Medium 248
An organic-rich, solid culture medium comprised of filtered, boiled rolled oats (rolled oats), trace elements, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium248.pdf 248. OAT FLAKES AGAR WITH YEAST EXTRACT Oat flakes 20.0 g Trace element (see medium 252) 1.0 ml Yeast extract 2.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 249
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium249.pdf 249. OROTIC ACID MEDIUM Tryptone 5.00 g Yeast extract 0.50 g KH2PO4 1.36 g K2HPO4 6.95 g Na-orotate 2.50 g Riboflavin 15.00 mg Resazurin 1.00 mg Na-thioglycolate 0.50 g Distilled water 1000.00 ml Adjust pH to 7.5. Gas atmosphere: 100% N2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, potassium phosphate, sodium orotate, riboflavin, resazurin, and sodium thioglycolate. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 250
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium250.pdf 250. PEPTONE MEAT EXTRACT GLYCEROL AGAR Proteose peptone no. 3 5.0 g Meat extract 3.0 g Glycerol 20.0 ml Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of proteose peptone, meat extract, glycerol, and agar.
DSMZ Medium 251
An organic-rich, solid culture medium comprised of proteose peptone, emat extract, glycerol, soil extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium251.pdf 251. PEPTONE - MEAT EXTRACT - SOIL EXTRACT AGAR (PFE) Proteose peptone no. 3 5.0 g Meat extract 3.0 g Glycerol 20.0 g Soil extract (see medium 80) 150.0 ml Agar 15.0 g Distilled water 850.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
Peptone - meat extract - soil extract agar
DSMZ Medium 253
An organic-rich, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium chloride, sodium chloride, sodium sulfate, sodium carbonate, trace elements, yeast extract, sodium succinate, sodium sulfide, vitamins, and sodium bicarbonate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium253.pdf 253. MEDIUM FOR ECTOTHIORHODOSPIRA KH2PO4 0.50 g CaCl2 x 2 H2O 0.05 g NH4Cl 0.80 g MgCl2 x 6 H2O 0.10 g NaCl 180.00 g Na2SO4 20.00 g Na2CO3 6.00 g Trace element solution (A) 1.00 ml Yeast extract 0.50 g Na-succinate 1.00 g Na2S x 9 H2O 1.00 g Distilled water 800.00 ml Adjust pH to 8.5. Sterilize at 120˚C for 15 minutes in screw-capped bottles. After sterilization add 1 ml/l filter-sterilized vitamin solution (B) and 200 ml of filter-sterilized solution (C). Trace element solution (A): FeCl2 x 4 H2O 1.80 g CoCl2 x 6 H2O 250.00 mg NiCl2 x 6 H2O 10.00 mg CuCl2 x 2 H2O 10.00 mg MnCl2 x 4 H2O 70.00 mg ZnCl2 100.00 mg H3BO3 500.00 mg Na2MoO4 x 2 H2O 30.00 mg Na2SeO3 x 5 H2O 10.00 mg Distilled water 1000.00 ml For dissolving adjust pH to about 3 with 1 N HCl. Vitamin solution (B): Biotin 10.0 mg Nicotinamide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml Bicarbonate solution (C): NaHCO3 14.0 g H2O © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 252
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf 252. STARCH - MINERAL SALT - AGAR (STMS) Starch, soluble 10.0 g (NH4)2SO4 2.0 g K2HPO4 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g CaCO3 2.0 g Trace element solution (see medium 84) 1.0 ml Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of soluble starch, ammonium sulfate, potassium phosphate, magnesium sulfate, sodium chloride, calcium carbonate, trace elements, and agar.
DSMZ Medium 255.1
Similar to DSMZ Medium 255, except cellobiose is omitted and glucose is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium255.pdf 255. CLOSTRIDIUM (GS) MEDIUM KH2PO4 0.50 g K2HPO4 x 3 H2O 1.00 g Urea 2.00 g MgCl2 x 6 H2O 0.50 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 1.25 mg Morpholinopropane sulfonic acid 10.00 g Resazurin 0.50 mg Yeast extract 6.00 g L-Cysteine-HCl x H2O 1.00 g Cellobiose 5.00 g Distilled water 1000.00 ml Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 – 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. For DSM 2360 replace cellobiose with 5.00 g/l D-glucose added to the medium after autoclaving from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved
DSM 2360 is Ruminiclostridium thermocellum DSM 2360
DSMZ Medium 255
An organic-rich, liquid culture medium comprised of potassium phosphate, urea, magnesium chloride, calcium chloride, ferrous sulfate, morpholinopropane sulfonic acid (MES), resazurin, yeast extract, L-cysteine hydrochloride, and cellobiose. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 254
An organic-rich, solid culture medium comprised of malt extract, yeast extract, agar, and ethanol.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium254.pdf 254. ACETOBACTER PEROXYDANS MEDIUM Malt extract 15.0 g Yeast extract 5.0 g Agar 15.0 g Distilled water 940.0 ml After sterilization add 60 ml ethanol (50% v/v), sterilized by filtration. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 256
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium256.pdf 256. THERMUS RUBER MEDIUM Universal peptone (Merck) 5.0 g Yeast extract 1.0 g Starch, soluble 1.0 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 8.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of universal peptone (M 66), yeast extract, soluble starch, and agar.
artificial sea water for DSMZ Medium 243
An atificial seawater comprised of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, and magnesium sulfate.
filtered, boiled rolled oats
trace elements solution for DSMZ Medium 84
A trace elements solution comprised of ferrous sulfate, manganese choride, and zinc sulfate.
trace elements solution A for DSMZ Medium 253
A trace elements solution comprised of ferrous chloride, cobalt chloride, nickel chloride, copper chloride, manganese chloride, zinc chloride, boric acid, sodium molybdate, and sodium selenite.
vitamin solution B for DSMZ Medium 253
A vitamin solution comprised of biotin, nicotinamide, thiamine hydrochloride, p-aminobenzoic acid (4-aminobenzoic acid), pyridoxal hydrochloride, calcium pantothenate, and vitamin B12 (cobalamin).
DSMZ Medium 300
Similar to DSMZ Medium 298, except butanediol is omitted and sodium L-tartrate, biotin, and yeast extract are added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium300.pdf 300. RUMINOCOCCUS PASTEURII MEDIUM Use medium 298, but replace butanediol by 2.0 g/l of sodium L-tartrate. Add 0.4 mg/l biotin or 1.0 g/l of yeast extract. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 301
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium301.pdf 301. FALCIVIBRIO MEDIUM Wilkins-Chalgren-Anaerobe-Broth (Oxoid) is supplemented with 0.3 g/l of L-cysteine and 1.0 mg/l of resazurin. Prepare the medium under 100% nitrogen gas. To the autoclaved medium add 50 ml/l of sterile serum (horse, bovine or lamb). © 2007 DSMZ GmbH - All rights reserved
Similar to Wilkins-Chalgren Anaerobe broth, except L-cysteine, rezasurin, and serum are added. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 298b
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298b.pdf 298b. SYNTROPHOBOTULUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Na-acetate 0.20 g Resazurin 0.50 mg NaHCO3 2.50 g Na2-glyoxalate 0.60 g Vitamins solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, glyoxalate, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add glyoxalate (sterilized by filtration), vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: When growth has started, feed culture once or twice with the same amount of glyoxalate. For DSM 6945 replace glyoxalate with 1.00 g/l Na-glycolate. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, sodium acetate, resazurin, sodium bicarbonate, sodium glyoxalate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 298b.1
DSM 6945 is an unclassified bacterium. No sequences in TaxBrowser.
Similar to DSMZ Medium 198b, except glyoxalate is omitted and sodium glycolate is added.
DSMZ Medium 298a
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, yeast extract, trace elements, selenite-tungstate, resazurin, sodium bicarbonate, sodium lactate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298a.pdf 298a. LuPhet1 MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Yeast extract 1.00 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg NaHCO3 5.00 g Na-DL-lactate 1.50 g Vitamins solution (see medium 503) 1.00 ml Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, lactate, vitamins, and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic tubes or bottles under the same gas atmosphere and autoclave. Add vitamins (sterilized by filtration), lactate and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 295
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, sodium nitrate, magnesium chloride, potassium chloride, calcium chloride, trace elements, selenite-tungstate, resazurin, sodium carbonate, D-glucose, vitamins, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium295.pdf 295. OPITUTUS MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g NaNO3 0.80 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Na2CO3 1.00 g D-Glucose 1.00 g Vitamins solution (see medium 141) 1.00 ml L-Cysteine-HCl x H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, glucose, vitamins, and cysteine. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add vitamins, glucose and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.3 - 7.5. For DSM 11249 replace glucose with 1.00 g/l cellobiose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. © 2014 DSMZ GmbH - All rights reserved
Thermoproteus tenax polar lipid fraction
Archaea cell extract, where the archaeon is Thermofilum pendens, and the cell extract is comprised of the lipid fraction of the cell.
http://www.sciencedirect.com/science/article/pii/S0723202083800356 Zillig G, Gierl A, Schreiber G, Wunderl S, Janekovic D, Stetter KO, Klenk HP. 1983. The Archaebacterium Thermofilum pendens Represents, a Novel Genus of the Thermophilic, Anaerobic Sulfur Respiring Thermoproteales. Syst Appl Microbiol 4(1):79-87. The growth of the organism requires peptides, sulfur and H2S and, in addition, a fraction of the polar lipids of the distantly related archaebacterium Thermoproteus tenax devoid of phosphate. This fraction cannot be replaced by an analogous fraction from Thermoplasma acidophilum.
Blood medium ingredient comprised of blood that has been definbrinated (where fibrin has been removed from the blood). Used in the cultivation of microorganisms.
DSMZ Medium 269.1
DSMZ Medium 269.1 -< for DSM 11237 and DSM 11244
Similar to DSMZ Medium 269, except the concentration of yeast extract is decreased and the pH is increased to 3.5.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium269.pdf 269. ACIDIPHILIUM MEDIUM (NH4)2SO4 2.0 g KCl 0.1 g K2HPO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.3 g D-Glucose 1.0 g Distilled water 1000.0 ml Autoclave glucose and yeast extract separately as a 10% and 3%, resp., solution. Adjust pH of completed medium to 3.0 with 1 N H2SO4. For DSM 11237 and DSM 11244 reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. For DSM 11245 replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. For DSM 100189 replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of completed medium to 2.5. © 2015 DSMZ GmbH - All rights reserved
DSM 11237 is Acidocella aminolytica 101 = DSM 11237 DSM 11244 is Acidobacterium capsulatum ATCC 51196
DSMZ Medium 269.2
Similar to DSMZ Medium 269, except yeast extract is omitted, and trypticase soy broth is added. The pH is increased to 3.5.
DSMZ Medium 269.3
Similar to DSMZ Medium 269, except glucose is omitted and galactose and ferrous sulfate are added. The pH is decreased to 2.5.
DSM 100189 is not in www.dsmz.de or in TaxBrowser or StrainInfo.
DSMZ Medium 269
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium chloride, potassium phosphate, magnesium sulfate, yeast extract, and D-glucose.
DSMZ Medium 265
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium265.pdf 265. THERMOFILUM PENDENS MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 x 7 H2O 0.01 mg Yeast extract 2.00 g Sucrose 2.00 g Sulfur, powdered 10.00 g Polar lipid fraction prepared from Thermoproteus tenax (DSM 2078) or from any other archaebacterium, aqueous suspension 6 - 12.00 ml Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Adjust final pH to 5.2. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for few minutes, not autoclaved; sucrose (20 ml of 10% w/v solution)-filter-sterilized; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; polar lipid fraction (6 - 12ml)-prepared as described by W. Zillig et al. (1983), Syst. Appl. Microbiol. 4: 79 - 87; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, yeast extract, sucrose, elemental sulfur, sodium sulfide, and the polar lipid fraction of Thermoproteus tenax. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 266
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium266.pdf 266. THERMOCOCCUS CELER MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 x 7 H2O 0.01 mg NaCl 40.00 g Resazurin 1.00 mg Yeast extract 2.00 g Sulfur, powdered 5.00 g Distilled water 1000.00 ml Adjust final pH to 5.8. Prepare the medium anaerobically under 100% nitrogen. The following constituents are prepared separately and added to the autoclaved mineral salt solution: Yeast extract (20 ml of 10% w/v solution)-boiled for a few minutes not autoclaved; sulfur (10 g)-sterilized by steaming for 3 h on each of three successive days; Na2S x 9 H2O (10 ml of 3% w/v solution)-autoclaved under nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfte, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, sodium choride, resazurin, yeast extract, and elemental sulfur. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 264
An organic-rich, liquid culture medium comprised of casein peptone, tryptic digested (casitone), yeast extract, filtered tomato juice, and Tween 80 (polysorbate 80).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium264.pdf 264. TOMATO JUICE MEDIUM Casein peptone, tryptic digested 10.0 g Yeast extract 10.0 g Filtered tomato juice 200.0 ml (pH 7.0) Tween 80 1.0 ml Distilled water 800.0 ml Adjust pH to 6.5. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 262
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, glucose, potassium phosphate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium262.pdf 262. J - AGAR Tryptone 5.0 g Yeast extract 15.0 g Glucose 2.0 g K2HPO4 3.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3 - 7.5. Sterilize glucose separately and add after sterilization. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 295, except glucose is omitted and cellobiose is added.
DSMZ Medium 261
Medium for chemilithotrophic growth of Bacillus schlegelii
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium261.pdf 261. MEDIUM FOR CHEMOLITHOTROPHIC GROWTH OF BACILLUS SCHLEGELII Use medium 260 without pyruvate. Incubate at 65˚C without agitation under 5% (v/v) O2, 10% CO2, 45% H2. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 260, except sodium pyruvate is omitted. Prepared under an atmosphere of dioxygen, carbon dioxide, and dihydrogen.
DSMZ Medium 259
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium259.pdf 259. YEAST EXTRACT MINERAL MEDIUM Na2HPO4 x 12 H2O 3.50 g K2HPO4 1.00 g MgSO4 x 7 H2O 0.03 g NH4Cl 0.50 g Yeast extract 4.00 g Agar 15.00 g Distilled water 1000.00 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, ammonium chloride, yeast extract, and agar.
DSMZ Medium 260
An organic-rich, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, manganese sulfate, magnesium sulfate, calcium chloride, ferric ammonium citrate, trace elements, and sodium pyruvate.
Bacillus schlegelii heterotrophic medium
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium260.pdf 260. BACILLUS SCHLEGELII HETEROTROPHIC MEDIUM Na2HPO4 x 2 H2O 4.50 g KH2PO4 1.50 g NH4Cl 1.00 g MnSO4 x H2O 0.01 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.01 g Ferric ammonium citrate 5.00 mg Trace element solution SL-6 3.00 ml (see medium 27) Na-Pyruvate 1.50 g Distilled water 1000.00 ml Adjust pH to 7.1. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121˚C. Incubate without agitation at 65˚C. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 238
Similar to DSMZ Medium 1, except streptomycin sulfate is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium238.pdf 238. STREPTOMYCIN NUTRIENT AGAR To medium 1 add 40 mg/l streptomycin sulfate. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 239
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium239.pdf 239. DIETHYL PHOSPHONATE MEDIUM KCl 0.20 g MgSO4 x 7 H2O 0.20 g NH4Cl 0.20 g Tris(hydroxymethyl)methylamine 6.00 g p-Hydroxybenzoate, Na-salt 0.75 g Agar 12.00 g Distilled water 900.00 ml Adjust pH to 7.4. After sterilization add 100 ml of a filter-sterilized solution of 1 mM diethyl phosphonate (Eastman Organic Chemical Div., Rochester, N.Y., USA) or of 20 mM phosphate buffer. © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, solid culture medium comprised of potassium chloride, magnesium sulfate, ammonium chloride, tris(hydroxymethyl)methylamine (tris), sodium p-hydroxybenzoate (sodium 4-hydroxybenzoate), agar, and diethyl phosphonate or a potassium or sodium phosphate buffer solution.
DSMZ Medium 242
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium242.pdf 242. BMM - MEDIUM (NH4)2SO4 3.0 g KH2PO4 2.0 g K2HPO4 7.0 g NaCl 2.0 g MgSO4 x 7 H2O 0.5 g Yeast extract 0.1 g Biotin 10.0 μg Thiamine-HCl x 2 H2O 100.0 μg MnSO4 x H2O 6.2 mg FeSO4 x 7 H2O 10.0 mg Methanol 10.0 ml Agar (if necessary) 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2 prior to autoclaving. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of ammonium sulfate, potassium phosphate, sodium chloride, magnesium sulfate, yeast extract, biotin, thiamine hydrochloride, manganese sulfate, methanol, and agar.
DSMZ Medium 240
Similar to DSMZ Medium 53, except defibrinated blood is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium240.pdf 240. CORYNEBACTERIUM MEDIUM WITH BLOOD Sterilize medium 53 and cool to about 45˚C. Aseptically add 5% defibrinated blood. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 245
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium245.pdf 245. BLOOD AGAR II Medium 220 supplemented with 5% defibrinated sheep blood. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 220, except defibrinated sheep blood is added.
Universal Peptone M 66
From: http://www.emdmillipore.com/US/en/product/Universal-peptone-M-66,MDA_CHEM-107043#documentation (See Universal Peptone M66 document under Supporting Documentation). Mode of Action Universal M 66 peptone is a mixture of casein peptone and meat peptone. Universal peptone M 66 combines the nutritive characteristics of casein and meat peptone. The well balanced peptone mix containing a representative mix of low and high molecular peptones, a broad range of free amino acids in growth supporting concentrations, vitamins and other growth factors. Universal peptone M 66 can be used as nutrient, for example as replacement of serum albumin, in cell culture and in fermentations. As an ingredient in culture media Universal Peptone M66 can be employed in media for the cultivation of a wide range of fastidious bacteria.
A microbiological medium ingredient, derived from enzymatic hydrolysis of mammalian protein. A mixture of casein peptone (casitone) and meat peptone.
DSMZ Medium 298
Pelobacter propionicus freshwater medium
Pelobacter propionicus medium (freshwater)
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, 2,3-butanediol (butane-2,3-diol), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium298.pdf 298. PELOBACTER PROPIONICUS MEDIUM (FRESHWATER) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g 2,3-butanediol 0.90 g Na2S x 9 H2O 0.36 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, 2,3-butanediol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes or serum vials under the same gas atmosphere and autoclave. Add 2,3-butanediol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For DSM 2395 replace 2,3-butanediol with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 2612M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 0.86 g/l crotonic acid added after autoclaving from an anoxic sterile stock solution neutralized with NaOH. For DSM 2805M supplement medium with 1.00 ml/l of a vitamins solution (see medium 503) and replace 2,3-butanediol by 1.25 g/l sodium pyruvate added after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 3246 replace 2,3-butanediol with 1.00 g/l acetoin. For DSM 6652 replace 2,3-butanediol with 2.60 g/l Na-glutarate and add 20.00 ml/l rumen fluid, clarified (see medium 1310) to the autoclaved medium. For DSM 6832 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace 2,3-butanediol by 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. For DSM 8385 supplement medium with 1.00 g/l yeast extract and replace 2,3-butanediol by 2.00 g/l D-galactose added from an anoxic stock solution sterilized by filtration. For DSM 8909 supplement medium with 1.00 ml/l of a vitamin solution (see medium 503) and replace 2,3-butanediol by 1.40 g/l triethanolamine hydrochloride added from a 1 M stock solution prepared and autoclaved under 100% N2 gas). For DSM 11264 supplement medium with 0.20 g/l yeast extract and 10.00 ml/l of a vitamins solution (see medium 141). Replace 2,3-butanediol by 1.24 g/l ethylene glycol added from a separately sterilized stock solution. For DSM 17541 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and 1.00 ml/l of selenite-tungstate solution (see medium 385). For DSM 29698 replace 2,3-butanediol with 2.20 g/l Na-gluconate. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 298.1
Similar to DSMZ Medium 298, except butanediol is omitted and poly(ethylene glycol) is added.
DSMZ Medium 298.2
Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and crotonic acid are added.
DSMZ Medium 298.3
Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and sodium pyruvate are added.
DSMZ Medium 298.4
Similar to DSMZ Medium 298, except butanediol is omitted and acetoin is added.
DSMZ Medium 298.5
Similar to DSMZ Medium 298, except butanediol is omitted and sodium glutarate and clarified rumen fluid are added.
DSMZ Medium 298.6
Similar to DSMZ Medium 298, except butanediol is omitted and vitamins and quinic acid are added.
DSMZ Medium 298.7
Similar to DSMZ Medium 298, except butanediol is omitted and yeast extract and D-galactose are added.
DSMZ Medium 298.8
Similar to DSMZ Medium 298, except butanediol is omitted and seven vitamins solution and triethanolamine hydrochloride are added.
DSM 8909 is Acetobacterium (no sequences in TaxBrowser)
DSMZ Medium 298.9
Similar to DSMZ Medium 298, except butanediol is omitted and yeast extract, vitamins and ethylene glycol are added.
DSMZ Medium 298.10
Similar to DSMZ Medium 298, except vitamins and selenite-tungstate are added.
DSM 17541 is anaerobic syntrophic bacterium NE23-3
DSMZ Medium 298.11
DSM 29698 is Clostridiales bacterium GluBS11
Similar to DSMZ Medium 298, except butanediol is omitted and sodium gluconate is added.
DSMZ Medium 294
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium294.pdf 294. HQGö1 MEDIUM KH2PO4 0.20 g NH4Cl 0.25 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 1.00 g Agar (for semi-solid media) 2.70 g Resazurin 0.50 mg NaHCO3 2.50 g Vitamin solution (see medium 503) 1.00 ml Gentisic acid 0.30 g Na2S x 9 H2O 0.10 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, gentisic acid and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in culture vessels and autoclave. Add vitamins, gentisic acid (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. If necessary, adjust the final pH of the medium to 7.2 with a bicarbonate stock solution. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth at the beginning. For transfers use 5 - 10% inoculum. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, selenite-tungstate, yeast extract, agar, resazurin, sodium bicarbonate, vitamins, gentisic acid (2,5-dihydroxybenzoic acid), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 293
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium293.pdf 293. PROPIONIGENIUM MODESTUM MEDIUM (MARINE) KH2PO4 0.20 g NH4Cl 0.25 g NaCl 20.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Na2-succinate 3.25 g Na2S x 9 H2O 0.36 g Distilled Water 1000.00 ml Dissolve ingredients except bicarbonate, succinate and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium in anoxic Hungate-type tubes under the same gas atmosphere and autoclave. Add succinate (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.2 - 7.5. For DSM 2376 supplement medium with 0.10% yeast extract (separately sterilized). For DSM 2377 and DSM 2378 replace Na2-succinate with 0.68 g/l of gallic acid. For DSM 2380 replace Na2-succinate with 0.90 g/l of 2,3-butanediol. For DSM 2382 replace Na2-succinate with 2.00 g/l of sodium L-tartrate. For DSM 2394 replace Na2-succinate with 1.00 g/l of polyethylene glycol (molecular weight 106 - 20000). For DSM 3247 replace Na2-succinate with 1.00 g/l acetoin. For DSM 6831 supplement medium with 10.00 ml/l of a vitamin solution (see medium 141) and replace Na2-succinate with 1.00 g/l of quinic acid added from a neutralized anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, sodium succinate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 293.1
Similar to DSMZ Medium 293, except yeast extract is added.
DSMZ Medium 293.2
Similar to DSMZ Medium 293, except sodium succinate is omitted and gallic acid is added.
DSM 2377 is Pelobacter acidigallici DSM 2378 is Pelobacter acidigallici
DSMZ Medium 293.2 -< for DSM 2377 and DSM 2378
DSMZ Medium 293.3
DSM 2380 is Pelobacter carbinolicus DSM 2380
Similar to DSMZ Medium 293, except sodium succinate is omitted and 2,3-butanediol (butane-2,3-diol) is added.
DSMZ Medium 293.4
Similar to DSMZ Medium 293, except sodium succinate is omitted and sodium L-tartrate is added.
DSMZ Medium 293.5
Similar to DSMZ Medium 293, except sodium succinate is omitted and polyethylene glycol (poly(ethylene glycol)) is added.
DSMZ Medium 293.6
Similar to DSMZ Medium 293, except sodium succinate is omitted and acetoin is added.
DSMZ Medium 293.7
Similar to DSMZ Medium 293, except sodium succinate is omitted and vitamins and quinic acid are added.
DSMZ Medium 292
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium292.pdf 292. ACIDAMINOBACTER MEDIUM Glycine 1.50 g Na2SO4 3.00 g KH2PO4 0.20 g MgCl2 x 6 H2O 0.40 g NaCl 1.20 g CaCl2 x 2 H2O 0.15 g KCl 0.40 g Yeast extract 0.20 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg NaHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins and sulfide), adjust pH to 6.5, then sparge with 100% N2 gas for 30 – 45 min to make it anoxic and dispense under same gas atmosphere in anoxic tubes or bottles. After autoclaving complete the medium by adding vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are sterilized by filtration. Adjust pH of the completed medium to 7.4 - 7.5. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of glycine, sodium sulfate, potassium phosphate, magnesium chloride, sodium chloride, calcium chloride, potassium chloride, yeast extract, trace elements, selenite-tungstate, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 291
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium291.pdf 291. PYROBACULUM NEUTROPHILUM MEDIUM Prepare medium 88 without yeast extract, then add per liter: Sulfur, powdered 8.0 g Resazurin 0.4 mg Adjust pH to 6.5 with NaOH, bring to boil for 5 min, then cool to room temperature under H2 + CO2 (80% + 20%) gas mixture. Add 0.85 g NaHCO3 per liter and equilibrate the pH to c. 6.5 by further gassing. Dispense in serum bottles (10 ml/30 ml bottle), seal and heat the bottles for one hour at 85˚C on each of three successive days. Before inoculation, add from anaerobic stock solutions 0.02% yeast extract (for mixotrophic growth only) and 0.01 ml Na-dithionite solution (250 mg Na-dithionite/10 ml dist. water, filter-sterilized). After inoculation, pressurize to 1 bar H2 + CO2 overpressure. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 88, except elemental sulfur, resazurin, sodium hydroxide, sodium dithionite and sodium bicarbonate are added. The pH is is increased to 6.5. Prepared under an atmosphere of dihydrogen and carbon dioxide.
DSMZ Medium 289
An organic-rich, liquid culture medium comprised of filtered sea water, mineral salt solution, ammonium chloride, yeast extract, potassium phosphate, resazurin, L-cysteine hydrochloride, and cellobiose. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium289.pdf 289. CLOSTRIDIUM PAPYROSOLVENS MEDIUM Sea water, filtered 200.00 ml Mineral salt solution (see below) 150.00 ml NH4Cl 1.00 g Yeast extract 0.60 g K2HPO4 1.65 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g Cellobiose 5.00 g Distilled water 650.00 ml Dissolve ingredients (except cysteine and cellobiose), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere and add cysteine. Adjust pH to 7.2 and dispense under 100% N2 gas in culture vessels and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. Mineral salt solution: (NH4)2SO4 6.00 g NaCl 6.00 g MgSO4 x 7 H2O 1.20 g CaCl2 x 2 H2O 0.80 g Distilled water 1000.00 ml © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 287
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, calcium chloride, magnesium sulfate, trace elements, ammonium chloride, sodium acetate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium287.pdf 287. METHANOGENIUM OLENTANGYI MEDIUM K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 0.61 g CaCl2 x H2O 0.14 g MgSO4 x 7 H2O 0.13 g Trace element solution (see medium 141) 10.00 ml NH4Cl 2.70 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and then dispense medium in anoxic Hungatetype tubes or serum bottles under 80% H2 and 20% CO2 gas atmosphere and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at one atmospheres of pressure. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 288
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium288.pdf 288. METHANOCOCCUS DELTAE MEDIUM K2HPO4 0.30 g KH2PO4 0.30 g (NH4)2SO4 0.30 g NaCl 35.00 g MgCl2 x 6 H2O 4.00 g CaCl2 x H2O 0.14 g Trace element solution (see medium 141) 10.00 ml NH4Cl 2.70 g Na-acetate 2.50 g Resazurin 0.50 mg NaHCO3 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, adjust pH to 6.8 and then dispense medium in anoxic Hungatetype tubes or serum bottles under 80% H2 and 20% CO2 gas atmosphere and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 – 7.0. For incubation use 80% H2 and 20% CO2 gas mixture at one atmospheres of pressure. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, sodium acetate, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
DSMZ Medium 286
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium286.pdf 286. CLOSTRIDIUM AMINOBUTYRICUM MEDIUM KH2PO4 1.30 g K2HPO4 7.00 g MgCl2 x 6 H2O 0.20 g FeCl3 x 6 H2O 0.01 g CaCl2 x 2 H2O 0.01 g Na2MoO4 x 2 H2O 1.00 mg Yeast extract 3.00 g gamma-Aminobutyric acid 5.00 g Agar 1.50 g Resazurin 0.50 mg Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except sulfide), adjust pH to 7.2, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under N2. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of potassium phosphate, magnesium chloride, ferric trichloride, calcium chloride, sodium molybdate, yeast extract, gamma-aminobutyric acid, agar, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 284
An organic-rich, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium selenite, sodium chloride, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, yeast extract, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium284.pdf 284. KOSMOTOGA MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg Na2SeO3 x 5 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 2.50 g Yeast extract 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, yeast extract, vitamins, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add vitamins (sterilized by filtration), yeast extract, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 7.0 – 7.2, if necessary. After inoculation add sterile 80% N2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 283
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium283.pdf 283. PYRODICTIUM MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Yeast extract 2.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sodium sulfide and adjust pH to 5.5 with 10 N sulfuric acid, boil and then cool under a stream of 80% H2 and 20% CO2 gas atmosphere. Add sodium sulfide, adjust the pH to 5.5, and distribute in appropriate vessels under 80% H2 and CO2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour before inoculation. For storage of medium at room temperature heat vessels at least on 2 successive days. Do not autoclave! After inoculation pressurize the vessels to 200 - 300 kPa overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 2709 reduce amount of yeast extract to 0.20 g/l. For DSM 6158 reduce amount of yeast extract to 0.50 g/l and omit citric acid. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, yeast extract, elemental sulfur, resazurin, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide.
DSMZ Medium 283.1
Similar to DSMZ Medium 283, except the concentration of yeast extract is decreased.
DSMZ Medium 283.2
Similar to DSMZ Medium 283, except the concentration of yeast extract is decreased and citric acid is omitted.
DSMZ Medium 282a
A minerals-salts, liquid culture medium comprised of potassium phosphate, calcium chloride, ammonium chloride, magnesium sulfate, magnesium chloride, potassium chloride, nickel chloride, sodium chloride, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282a.pdf 282a. METHANOCALDOCOCCUS VILLOSUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 18.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 282
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium282.pdf 282. METHANOCALDOCOCCUS MEDIUM K2HPO4 0.14 g CaCl2 x 2 H2O 0.14 g NH4Cl 0.25 g MgSO4 x 7 H2O 3.40 g MgCl2 x 6 H2O 4.10 g KCl 0.33 g NiCl2 x 6 H2O 0.50 mg NaCl 30.00 g Fe(NH4)2(SO4)2 x 6 H2O 0.01 g Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 1.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Note: Some strains grow within a few hours to stationary phase, thus check growth in regular intervals and do not incubate over night. For DSM 5666 adjust pH of completed medium to 6.5. For DSM 16983 adjust pH to 7.0 – 7.2. For DSM 26965 supplement medium with 3.00 g/l yeast extract and add 2.50 g/l maltose to the autoclaved medium from a sterile anoxic stock solution prepared under 100% N2 gas. Adjust pH of completed medium to 6.5 and use without overpressure of sterile 80% H2 and 20% CO2 gas mixture. For DSM 27223 adjust pH of completed medium to 7.0. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 282.1
Similar to DSMZ Medium 282, except the pH is increased to 6.5.
DSMZ Medium 282.2
DSM 16983 is Methanotorris formicicus Mc-S-70
Similar to DSMZ Medium 282, except the pH is increased to 7.0-7.2.
DSMZ Medium 282.3
Similar to DSMZ Medium 282, except yeast extract and maltose are added. The pH is increased to 6.5.
DSMZ Medium 282.4
Similar to DSMZ Medium 282, except the pH is increased to 7.0.
DSM 27223 is Methanocaldococcus bathoardescens
DSMZ Medium 281
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium281.pdf 281. HYPHOMONAS MEDIUM Casitone (Difco) 2.0 g Yeast extract (Difco) 1.0 g MgCl2 x 6 H2O 2.0 g Distilled water 1000.0 ml Adjust pH to 7.5 (use indicator paper). © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of casitone, yeast extract, and magnesium chloride.
DSMZ Medium 280.4
Similar to DSMZ Medium 280, except trimethylamine is omitted and methanol added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium280.pdf 280. METHANOCOCCOIDES MEDIUM (N2/CO2) KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Trimethylamine x HCl 3.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, trimethylamine, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trimethylamine, cysteine and sulfide from sterile anoxic stock solutions autoclaved under 100% N2 gas. Vitamins are prepared under N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 7.0 - 7.2. For DSM 2831 replace trimethylamine with 5.00 g/l sucrose added from a sterile anoxic stock solution prepared under N2. For DSM 4138 replace trimethylamine with 5.00 g/l D-glucose added from a sterile anoxic stock solution prepared under N2. Use an overpressure of 2 bar of 80% N2 and 20% CO2 gas mixture. For DSM 11916 adjust pH to 6.6. For DSM 14042 replace trimethylamine with 0.50 ml/l methanol added from a sterile anoxic stock solution prepared under N2 gas. For incubation use 80% N2 and 20% CO2 gas mixture at two atmospheres of pressure. For DSM 16625 supplement the autoclaved medium with 3.00 g/l Na2S2O3 from a sterile anoxic stock solution. © 2014 DSMZ GmbH - All rights reserved
DSM 14042 is Methanosarcina baltica GS1-A
DSMZ Medium 280.3
DSM 11916 is Methanosarcina siciliae C2J
Similar to DSMZ Medium 280, except the pH is decreased to 6.6.
DSMZ Medium 280.2
Similar to DSMZ Medium 280, except trimethylamine is omitted and glucose is added. The partial pressures of dinitrogen and carbon dioxide are increased.
DSMZ Medium 280.1
Similar to DSMZ Medium 280, except trimethylamine is omitted and sucrose is added.
DSM 2831 is not in TaxBrowser or StrainInfo.
DSMZ Medium 280.5
Similar to DSMZ Medium 280, except sodium thiosulfate is added.
DSM 16625 is Methanococcoides methylutens MM1
DSMZ Medium 280
An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypticase (trypticase peptone), resazurin, sodium bicarbonate, trimethylamine hydrochloride, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 277
An organic-rich, solid culture medium comprised of sour stone cherry extract and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium277.pdf 277. CHERRY AGAR (CBS formula) Add 1 l water to 200.0 g pulp of sour stone cherries. Heat to boiling and simmer gently for 2 h. Strain through cloth and sterilize at 110˚C for 30 minutes. Dissolve 20.0 g agar in 800 ml water and sterilize. Add 200 ml cherry extract, distribute in presterilized tubes and sterilize for 5 min at 120˚C. Final pH 3.8 - 4.6. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 276
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium276.pdf 276. HALOMONAS MEDIUM NaCl 80.00 g Casamino acids (with vitamins) 7.50 g Proteose peptone No. 3 5.00 g Yeast extract 1.00 g Na3-citrate 3.00 g MgSO4 x 7 H2O 20.00 g K2HPO4 0.50 g Fe(NH4)2(SO4)2 x 6 H2O 0.05 g Agar, if necessary 15.00 g Distilled water 1000.00 ml Before autoclaving adjust pH to 7.5 with KOH. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of sodium chloride, casamino acids, proteose peptone, yeast extract, sodium citrate, magnesium sulfate, potassium phosphate, potassium phosphate, ferrous ammonium citrate, and agar.
DSMZ Medium 275
An organic-rich, liquid culture medium comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, rumen fluid, yeast extract, peptone, ammonium sulfate, resazurin, cysteine hydrochloride, glucose, and sodium bicarbonate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium275.pdf 275. TREPONEMA SUCCINIFACIENS MEDIUM Solution A: CaCl2 x 2 H2O 0.100 g MgSO4 x 7 H2O 0.100 g KH2PO4 0.500 g K2HPO4 0.500 g NaCl 1.000 g Rumen fluid (clarified) 300.000 ml Yeast extract (Difco) 0.500 g Peptone (Difco) 0.500 g (NH4)2SO4 0.500 g Resazurin 0.001 g Cysteine-HCl x H2O 0.500 g Distilled water 575.000 ml Solution B: Glucose (20% w/v, heat-sterilized) 50.000 ml Solution C: Potassium phosphate buffer (1M, pH 7.4, heat-sterilized) 25.000 ml Solution D: NaHCO3 (5% w/v, filter-sterilized) 50.000 ml Final pH is 7.4. Prepare the medium anaerobically under 100% nitrogen atmosphere. Dissolve ingredients of part A, in the amount of water indicated, adjust pH to 6.2 - 6.3 with 4N HCl and heat to boiling point. Cool to room temperature under 100% nitrogen and distribute under the same gas in anaerobic tubes. Autoclave 20 minutes at 121˚C. After the broth had cooled, add appropriate amounts each of part B, C, and D, (all solutions prepared under nitrogen) to the tubes. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 274
An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, sodium bicarbonate, sodium acetate, resazurin, MOPS, vitamin solution, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium274.pdf 274. METHANOMICROBIUM PAYNTERI MEDIUM KCl 0.34 g MgCl2 x 2 H2O 2.75 g MgSO4 x 7 H2O 3.40 g NH4Cl 1.50 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 6.31 g Trace element solution (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg NaHCO3 5.00 g Na-acetate x 3 H2O 4.15 g Resazurin 0.50 mg MOPS (3-(N-morpholino) propane-sulfonic acid) 20.93 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except MOPS, vitamins, cysteine and sulfide in a total volume of 890 ml. The MOPS buffer is dissolved separately in 90 ml of distilled water, the pH is adjusted to 7.0 with 2 M KOH, then add the buffer to the salt solution. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense medium in anoxic Hungate-type tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 7.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 273
An organic-rich, liquid culture medium comprised of glucose, trypticase (trypticase peptone), yeast extract, tris, sea water, cysteine hydrochloride, and resazurin. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium273.pdf 273. SPIROCHAETA ISOVALERICA MEDIUM Glucose 2.000 g Trypticase (BBL) 1.000 g Yeast extract 0.500 g Tris-HCl-buffer (0.2 M; pH 7.5) 250.000 ml Sea water 750.000 ml Cysteine-HCl x H2O 0.050 g Resazurin 0.001 g Adjust pH to 7.5. Prepare the medium anaerobically under 100% nitrogen atmosphere. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 271
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium271.pdf 271. ACIDITHIOBACILLUS FERROOXIDANS (APH) MEDIUM (NH4)2SO4 2.00 g K2HPO4 0.50 g MgSO4 x 7 H2O 0.50 g KCl 0.10 g Ca(NO3)2 0.01 g FeSO4 x 7 H2O 8.00 g Distilled water 1000.00 ml Dissolve ingredients and adjust pH to 2.0 with 10 N H2SO4. Sterilize the medium by filtration. Alternatively, autoclave separately the basal medium (pH adjusted to 2.0) and the ferrous sulfate (8.00 g FeSO4 x 7 H2O in 50 ml distilled water, pH not adjusted, in a sealed vessel under nitrogen gas atmosphere). For DSM 11478 and DSM 24413: Omit the ferrous sulfate from the medium and add 10.00 g/l elemental sulfur as substrate. For sterilization place the sulfur in screw-capped tubes, add 1-2 drops of distilled water and incubate on 3 successive days for 3 h at 90-100˚C in a water bath. Before use, aseptically layer the sulfur onto the surface of autoclaved liquid basal medium. Adjust pH of completed medium to 2.0 for DSM 11478 and to 2.5 for DSM 24413. © 2015 DSMZ GmbH - All rights reserved
Acidithiobacillus ferrooxidans (APH) medium
A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, potassium chloride, calcium nitrate, and ferrous sulfate.
DSMZ Medium 271.1
Similar to DSMZ Medium 271, except ferrous sulfate is omitted and elemental sulfur is added.
DSMZ Medium 271.1 -< for DSM 11478 and DSM 24413
DSM 11478 is Acidithiobacillus thiooxidans DSM 24413 is Acidithiobacillus caldus
An extract of sour stone cherries (Prunus cerasus). Produces by heating in water, then straining and sterilizing the extract.
Wilkins-Chalgren Anaerobe Broth
http://www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0643&cat=&sec=1&c=UK&lang=EN WILKINS-CHALGREN ANAEROBE BROTH Code: CM0643 A medium for the general growth and antimicrobial susceptibility testing of anaerobic organisms. See Antimicrobial Susceptibility Testing Section 6 for details of the use of this medium in AST methodology. Typical Formula* gm/litre Tryptone 10.0 Gelatin peptone 10.0 Yeast extract 5.0 Glucose 1.0 Sodium chloride 5.0 L-Arginine 1.0 Sodium pyruvate 1.0 Menadione 0.0005 Haemin 0.005 pH 7.1 ± 0.2 @ 25°C * Adjusted as required to meet performance standards Directions Suspend 33g in 1 litre of warm distilled water. Mix well, distribute into final containers and sterilise by autoclaving at 121°C for 15 minutes. Description Wilkins-Chalgren Anaerobe Broth is derived from Wilkins-Chalgren Anaerobe Agar1 CM0619. Where studies on antimicrobial susceptibilities are being made both in broth and agar, standardisation is improved by using media of identical nutrient formulation. The growth of anaerobic organisms in this broth is particularly good. The formulation includes yeast extract to supply vitamins and other growth factors such as purines and pyrimidines, that are necessary for good growth of Peptostreptococcus anaerobius and Bacteroides melaninogenicus. Arginine is added to ensure sufficient amino-acid is available for the growth of Eubacterium lentum. Pyruvate is present as an energy source for asaccharolytic cocci such as Veillonella. It also acts similarly to catalase and degrades traces of hydrogen peroxide, which may be produced by the action of molecular oxygen on medium constituents and interfere with the metabolism of anaerobes. Haemin is found to be essential for the growth of Bacteroides species and menadione for Bacteroides melaninogenicus. Peptones derived from the single protein sources casein and gelatin are used to improve standardisation of the medium. The early heavy growth that is usual may reflect the absence of Eh-reducing substances that can be inhibitory to some organisms.
An organic-rich, liquid culture medium comprised of tryptone, gelatin peptone (pancreatic digest of gelatin), yeast extract, glucose, sodium chloride, L-arginine, sodium pyruvate, menadione, and hemin.
mineral salt solution for DSMZ Medium 289
An inorganic salts solution comprised of ammonium sulfate, sodium chloride, magnesium sulfate, and calcium chloride.
DSMZ Medium 329
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium329.pdf 329. METHANOHALOPHILUS HALOPHILUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g K2SO4 0.01 g NaCl 70.00 g Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 0.05 g Resazurin 0.50 mg NaHCO3 5.00 g MgCl2 x 6 H2O 0.33 g CaCl2 x 2 H2O 0.33 g Vitamin solution (see medium 141) 10.00 ml Trimethylamine-HCl 5.00 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.15 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, magnesium chloride, calcium chloride, vitamins, trimethylamine, cysteine, and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add bicarbonate and equilibrate pH to 6.9 - 7.0, then distribute under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium chloride, calcium chloride, vitamins (sterilized by filtration), trimethylamine, cysteine, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use adjust pH of completed medium to 7.2. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, potassium sulfate, sodium chloride, trace elements, yeast extract, resazurin, sodium bicarbonate, magnesium chloride, calcium chloride, vitamins, trimethylamine hydrochloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 328
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium328.pdf 328. PYG MEDIUM WITH VOLATILE FATTY ACIDS Beef extract 5.00 g Trypticase (BBL) 5.00 g Yeast extract 10.00 g Mineral solution (see below) 40.00 ml (NH4)2SO4 0.50 g Fatty acid mixture (see below) 3.10 ml Glucose 5.00 g Resazurin 1.00 mg Haemin (dissolved in 0.5 ml 1 N NaOH) 5.00 mg Vitamin K1 (0.5% sol. in ethanol) 0.20 ml Cysteine-HCl x H2O 0.50 g Distilled water 960.00 ml Adjust pH to 6.9. Dissolve ingredients except cysteine-HCl in water, boil for 5 min, cool to room temperature while gassing with 100% CO2, add cysteine-HCl and adjust pH to 6.9 with 8 N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under nitrogen, and autoclave. Mineral solution: CaCl2 x 2 H2O 0.30 g MgSO4 x 7 H2O 0.48 g K2HPO4 1.00 g KH2PO4 1.00 g NaHCO3 10.00 g NaCl 2.00 g Distilled water 1000.00 ml Fatty acid mixture: Acetic acid 17.0 ml Propionic acid 6.0 ml n-Butyric acid 4.0 ml n-Valeric acid 1.0 ml iso-Valeric acid 1.0 ml iso-Butyric acid 1.0 ml DL-2-Methylbutyric acid 1.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of beef extract, trypticase (trypticase peptone), yeast extract, mineral solution, ammonium sulfate, fatty acid mixture, glucose, resazurin, hemin, vitamin K1, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide.
mineral solution for DSMZ Medium 328
fatty acid mixture for DSMZ Medium 328
A fatty acid solution comprised of acetic acid, propionic acid, butyric acid, valeric acid, iso-valeric acid, iso-butyric acid, and 2-methylbutyric acid.
DSMZ Medium 309
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium309.pdf 309. NEOMYCIN AGAR Beef extract 1.5 g Yeast extract 3.0 g Peptone 6.0 g Glucose 1.0 g Casitone 4.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Aseptically add neomycin to a final concentration of 1.0 mg/ml of medium. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of beef extract, yeast extract, peptone, glucose, casitone, and neomycin.
DSMZ Medium 306
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium306.pdf 306. NY-AGAR To medium 1 add 0.5% yeast extract. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 1, except yeast extract is added.
DSMZ Medium 308
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium308.pdf 308. VIBRIO MEDIUM Tryptone 10.0 g NaCl 10.0 g MgCl2 x 6 H2O 4.0 g KCl 1.0 g Distilled water 1000.0 ml Adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, sodium chloride, magnesium chloride, and potassium chloride.
DSMZ Medium 305
An organic-rich, solid culture medium comprised of soluble starch, peptone, meat extract, yeast extract, potassium phosphate, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium305.pdf 305. BACILLUS THERMOGLUCOSIDASIUS MEDIUM Soluble starch 10.0 g Peptone 5.0 g Meat extract 3.0 g Yeast extract 3.0 g KH2PO4 3.0 g Agar 30.0 g Distilled water 1000.0 ml Adjust final pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 303
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium303.pdf 303. SIMONSIELLA MEDIUM Trypticase (BBL) 17.0 g Phytone (BBL) 3.0 g NaCl 5.0 g K2HPO4 2.5 g Yeast extract 4.0 g Horse serum 100.0 ml Agar, if necessary 15.0 g Distilled water 1000.0 ml Prepare the medium without horse serum. After autoclaving cool to 50˚C, then add 100 ml/l of sterile horse serum. Transfer cultures when actively growing. Cultures die rapidly in stationary growth phase! © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of trypticase (trypticase peptone), phytone (phytone peptone), sodium chloride, potassium phosphate, yeast xtract, horse serum, and agar.
DSMZ Medium 326
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium chloride, cobalt chloride, trace elements, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, sucrose, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium326.pdf 326. CLOSTRIDIUM STERCORARIUM (A-GÖ) MEDIUM KH2PO4 3.30 g MgCl2 x 6 H2O 0.16 g CoCl2 x 6 H2O 12.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 4.00 g Sucrose 5.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, sucrose, vitamins, cysteine, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add sucrose, cysteine and sulfide to the medium after autoclaving from sterile stock solutions autoclaved under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins are prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 6.8 – 7.2. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 323
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium323.pdf 323. TREPONEMA SACCHAROPHILUM MEDIUM Salt solution A 200.00 ml Salt solution B 200.00 ml Resazurin 1.00 mg Yeast extract (Oxoid) 2.00 g Trypticase (BBL) 2.00 g n-Butyric acid 0.40 ml iso-Butyric acid 0.40 ml DL-2-Methylbutyric acid 0.20 ml n-Valeric acid 0.20 ml iso-Valeric acid 0.20 ml Cysteine-HCl x H2O 1.00 g NaHCO3 5.00 g Glucose 2.00 g Distilled water 480.00 ml Adjust pH to 6.7 - 7.0. Gas atmosphere: 100% CO2. Dissolve ingredients except NaHCO3 and glucose in 880 ml water (including salt solutions). Adjust pH to 7.0 with KOH. Boil for 5min, then cool under CO2 gas to room temperature and distribute under CO2 gas atmosphere (8.8 ml/anaerobic culture tube). After autoclaving add 1 ml of a filter-sterilized NaHCO3 solution (5% w/v) and 0.2 ml of a filter-sterilized glucose solution (10% w/v) to each tube. Check final pH which should be 6.7 - 7.0. Salt solution A: CaCl2 x 2 H2O 0.59 g MgSO4 x 7 H2O 0.96 g Distilled water 1000.00 ml Salt solution B: KH2PO4 2.25 g K2HPO4 2.25 g NaCl 4.50 g (NH4)2SO4 4.50 g Distilled water 1000.00 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of calcium chloride, magnesium sulfate, potassium phosphate, sodium chloride, ammonium sulfate, resazurin, yeast extract, trypticase (trypticase peptone), butyric acid, isobutyric acid, 2-methylbutyric acid, valeric acid, isovaleric acid, cysteine hydrochloride, sodium bicarbonate, and glucose. Prepared under an atmosphere of carbon dioxide.
DSMZ Medium 325
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, resazurin, sodium bicarbonate, sodium caproate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium325.pdf 325. SYNTROPHOMONAS BRYANTII MEDIUM Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Trace element solution SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg NaHCO3 5.00 g Na-caproate 1.40 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, caproate, vitamins and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Dispense under same gas atmosphere in Hungate-type tubes or serum vials and autoclave. After sterilization add caproate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of final medium to 7.2 – 7.4. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 322
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium322.pdf 322. METHANOSPHAERA (MCB-3) MEDIUM Rumen fluid, clarified (see medium 1310) 100.00 ml Trypticase peptone (BD BBL) 2.00 g Yeast extract 2.00 g Na-acetate 0.50 g Na-formate 0.50 g Trace element solution (see medium 141) 10.00 ml Na2SeO4 1.90 mg NiCl2 x 6 H2O 0.70 mg FeSO4 x 7 H2O 3.00 mg K2HPO4 0.60 g KH2PO4 2.80 g (NH4)2SO4 0.30 g NH4Cl 1.00 g NaCl 0.60 g MgSO4 x 7 H2O 0.15 g CaCl2 x 2 H2O 0.08 g Resazurin 0.50 mg Methanol 5.00 ml Vitamin solution (see medium 141) 20.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.50 g Distilled water 900.00 ml Dissolve ingredients except methanol, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Adjust pH of medium to 6.8 – 7.0, then dispense in anoxic Hungate-type tubes under 80% H2 and 20% CO2 gas atmosphere and autoclave. Add methanol, vitamins (sterilized by filtration), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.7 - 6.9, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of clarified rumen fluid, trypticase peptone, yeast extract, sodium acetate, sodium formate, trace elements, sodium selenate, nickel chloride, ferrous sulfate, potassium phosphate, ammonium sulfate, ammonium chloride, sodium chloride, magnesium sulfate, calcium chloride, resazurin, methanol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
DSMZ Medium 321
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium321.pdf 321. METHANOCORPUSCULUM AGGREGANS MEDIUM NH4Cl 1.00 g K2HPO4 x 3 H2O 0.40 g MgCl2 x 6 H2O 0.40 g Mineral solution (see below) 50.00 ml Na-formate 5.00 g Na-acetate 1.00 g Trypticase (BD BBL) 1.00 g Yeast extract 1.00 g Trace element solution (see medium 141) 10.00 ml Sludge fluid (see medium 119) or 5.00 ml Rumen fluid, clarified (see medium 1310) 5.00 ml Resazurin 0.50 mg Na2CO3 1.50 g L-Cysteine-HCl x H2O 0.20 g Na2S x 9 H2O 0.20 g Distilled water 935.00 ml Dissolve ingredients except carbonate, cysteine and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use check pH of completed medium and adjust to 6.8 – 7.0, if necessary. After inoculation add sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, mineral solution, sodium formate, sodium acetate, trypticase (trypticase peptone), yeast extract, trace elements, sludge fluid, rumen fluid, resazurin, sodium carbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and carbon dioxide.
DSMZ Medium 319
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium319.pdf 319. SPOROHALOBACTER LORTETII MEDIUM Casamino acids 2.00 g Nutrient broth 2.00 g Yeast extract 2.00 g L-Glutamic acid 4.00 g NaCl 105.00 g KCl 0.75 g Trace element solution (see medium 141) 10.00 ml FeSO4 x 7 H2O 2.00 mg Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g MgCl2 x 6 H2O 10.00 g CaCl2 x 2 H2O 3.70 g Vitamin solution (see medium 141) 10.00 ml Distilled water 1000.00 ml Dissolve ingredients (except cysteine, magnesium chloride, calcium chloride and vitamins), bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Thereafter, add and dissolve cysteine, adjust pH to 6.5 and dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add magnesium chloride and calcium chloride from sterile anoxic stock solutions autoclaved under 100% N2 gas and vitamins from a filter-sterilized stock solution prepared under N2 gas. Note: For agar medium add 20.00 g/l agar, 5.00 g/l CaCO3, and 2.00 g/l soluble starch. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of casamino acids, nutrient broth, yeast extract, L-glutamic acid, sodium chloride, potassium chloride, trace elements, ferrous sulfate, resazurin, L-cysteine hydrochloride, magnesium chloride, calcium chloride, and vitamins. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 318c
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318c.pdf 318c. PELOBACTER ACIDIGALLICI MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BBL) 0.50 g Resazurin 0.50 mg KHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na-gallate 1.90 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, gallate, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, gallate, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and gallate are sterilized by filtration. Prior to use adjust pH of completed medium to 7.3. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamins, sodium gallate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 318b
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, yeast extract, trypticase (trypticase peptone), resazurin, potassium bicarbonate, vitamins, ethanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318b.pdf 318b. CLOSTRIDIUM NEOPROPIONICUM MEDIUM KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Yeast extract 0.50 g Trypticase (BD BBL) 0.50 g Resazurin 0.50 mg KHCO3 4.00 g Ethanol 1.30 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, ethanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add ethanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins are prepared under N2 gas and sterilized by filtration. Adjust final pH to 7.0 – 7.2, if necessary. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 318a
An organic-rich, liquid culture medium comprised of HEPES buffer, potassium phosphate, sodium chloride, magnesium chloride, calcium chloride, trace elements, ammonium chloride, resazurin, potassium bicarbonate, vitamins, 2-furoic acid, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium318a.pdf 318a. PELOBACTER (FUF) MEDIUM HEPES (SIGMA) 11.92 g KH2PO4 0.30 g NaCl 0.60 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.08 g Trace element solution (see medium 144) 10.00 ml NH4Cl 1.00 g Resazurin 0.50 mg KHCO3 2.00 g Vitamin solution (see medium 141) 10.00 ml 2-Furoic acid (SIGMA) 2.24 g L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate, vitamins, furoic acid, cysteine and sulfide and adjust pH of solution to 7.0. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add furoic acid (stock solution neutralized with NaOH), cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 7.0 – 7.2. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 317
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium317.pdf 317. METHANOSARCINA (MP) MEDIUM NH4Cl 1.00 g K2HPO4 0.40 g MgCl2 x 6 H2O 0.20 g Mineral solution (see below) 50.00 ml Trace element solution (see medium 141) 10.00 ml Yeast extract 1.00 g Resazurin 0.50 mg Na2CO3 1.00 g Methanol 5.00 ml L-Cysteine-HCl x H2O 0.50 g Na2S x 9 H2O 0.25 g Distilled water 950.00 ml Dissolve ingredients except carbonate, methanol, cysteine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense medium under the same gas atmosphere in anoxic Hungate-type tubes or serum vilas and autoclave. Add methanol, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of completed medium to 6.8 - 7.0. Minerals solution: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, mineral solution, trace elements, yeast extract, resazurin, sodium carbonate, methanol, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 316
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, sodium phosphate, magnesium chloride, trypticase (trypticase peptone), yeast extract, trace elements, vitamins, ferrous sulfate, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium316.pdf 316. MOORELLA THERMOACETICA (TYE-CO) MEDIUM NH4Cl 1.0 g KH2PO4 0.3 g Na2HPO4 x 12 H2O 2.8 g MgCl2 x 6 H2O 0.2 g Trypticase (BD BBL) 10.0 g Yeast extract 3.0 g Trace element solution (see medium 141) 10.0 ml Vitamin solution (see medium 141) 5.0 ml FeSO4 x 7 H2O 1.0 mg Resazurin 0.5 mg Na2S x 9 H2O 0.6 g Distilled water 1000.0 ml Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% carbon monoxide gas. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 315
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, ferrous sulfate, trace elements, vitamins, cellobiose or cellulose, resazurin, sodium bicarbonate, cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium315.pdf 315. BACTEROIDES CELLULOSOLVENS MEDIUM NH4Cl 0.68 g K2HPO4 0.30 g KH2PO4 0.18 g (NH4)2SO4 0.15 g MgSO4 x 7 H2O 0.12 g CaCl2 x 2 H2O 0.06 g FeSO4 x 7 H2O 0.02 g Trace element solution (see below) 10.00 ml Vitamin solution (see below) 10.00 ml Cellobiose 5.00 g or Cellulose (i.e. MN 300) 5.00 g Resazurin 1.00 mg NaHCO3 2.00 g Cysteine-HCl x H2O 0.25 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Adjust pH to 7.0. Gas atmosphere: 80% N2 + 20% CO2. Filter sterilize cellobiose separately. Trace element solution: Nitrilotriacetic acid 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g KAl(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg Distilled water 1000.000 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH). © 2009 DSMZ GmbH - All rights reserved
DSMZ Medium 314
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium314.pdf 314. ILYOBACTER POLYTROPUS MEDIUM NaCl 21.00 g MgCl2 x 6 H2O 3.10 g KCl 0.30 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.27 g KH2PO4 0.20 g Na2SO4 2.84 g Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Na-3-hydroxybutyrate 1.40 g Yeast extract 1.00 g Resazurin 0.50 mg NaHCO3 4.50 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.40 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, vitamins, and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium in anoxic Hungate-type tubes under 80% N2 and 20% CO2 gas atmosphere and autoclave. Add vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. The vitamin solution should be sterilized by filtration. Prior to use check pH of completed medium and adjust to 7.2 - 7.4, if necessary. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, potassium chloride, calcium chloride, ammonium chloride, potassium phosphate, sodium sulfate, trace elements, selenite-tungstate, sodium 3-hydroxybutyrate, yeast extract, resazurin, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 312
An organic-rich, liquid culture medium comprised of sucrose, casitone, yeast extract, trypticase soy broth without dextrose, thiamine hydrochloride, and vitamin B12 (cobalamin).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium312.pdf 312. SCY - MEDIUM Sucrose 1.00 g Casitone 0.75 g Yeast extract 0.25 g Trypticase soy broth without dextrose (BBL) 0.17 g Thiamine-HCl x 2 H2O 0.40 mg Vitamin B12 0.01 mg Distilled water 1000.00 ml Adjust pH to 7.1. Sterilize vitamins separately by filtration. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 311b
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311b.pdf 311b. SPOROMUSA MEDIUM (MARINE) NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 25.00 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.5 – 7.7. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, selenite-tungstate, yeast extract, casitone, betaine, resazurin, potassium phosphate, sodium bicarbonate, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 311a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311a.pdf 311a. SPOROMUSA MEDIUM (H2/CO2) NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml DL-Dithiothreitol 0.15 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins and dithiothreitol) and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins and dithiothreitol should be prepared under N2 gas and sterilized by filtration. Adjust pH of completed medium to pH 7.0. For DSM 11379, DSM 11380, DSM 11381 and DSM 11382 supplement medium with 2.50 g/l Na-pyruvate. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium sulfate, calcium chloride, sodium chloride, ferrous sulfate, trace elements, selenite-tungstate, yeast extract, casitone, resazurin, potassium phosphate, sodium bicarbonate, vitamins, and dithiothreitol (1,4-dithiothreitol). Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
DSMZ Medium 311a.1
Similar to DSMZ Medium 311a, except sodium pyruvate is added.
DSM 11379 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11380 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11381 is Sporomusa (not in StrainInfo, no sequences in TaxBrowser) DSM 11382 is Acetonema sp. DSM 11382
DSMZ Medium 311a.1 -< for DSM 11379, DSM 11380, DSM 11381 and DSM 11382
DSMZ Medium 310
An organic-rich, solid culture medium comprised of V-8 juice, calcium carbonate, and agar.
Salinity derived from the V-8 juice is 1.4g/L NaCl, or 0.14% NaCl).
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium310.pdf 310. V-8 JUICE AGAR *V-8 Juice 200.0 ml CaCO3 3.0 g Agar 15.0 g Fill up with distilled water to 1000.0 ml. Adjust pH to 7.2. * May be replaced by tomato mash if not available. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 336
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, yeast extract, sodium vanillate, vitamins, clarified rumen fluid, resazurin, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium336.pdf 336. OXOBACTER PFENNIGII MEDIUM Solution A: Mineral solution (see medium 335) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Na-vanillate 2.00 g Vitamin solution (see medium 503) 1.00 ml Rumen fluid, clarified (see medium 1310)) 300.00 ml Resazurin 0.50 mg Distilled water 540.00 ml Adjust pH to 6.9 before autoclaving. Solution B: NaHCO3 5.00 g Distilled water 100.00 ml Solution C: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution D: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute under same gas atmosphere 8.9 ml medium per Hungate-type tube and autoclave. Filter sterilize solution B and sparge for 20 min with 80% N2 and 20% CO2 gas mixture to make it anoxic. Autoclave solutions C and D under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.2. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 337
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium337.pdf 337. SYNTROPHOCOCCUS SUCROMUTANS MEDIUM Solution A: Mineral solution (see medium 335) 50.00 ml Trace element solution (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 300.00 ml Trypticase (BBL) or Casitone peptone 5.00 g Na-formate 0.60 g Resazurin 0.50 mg Distilled water 560.00 ml Solution B: NaHCO3 2.50 g Distilled water 50.00 ml Solution C: Vitamin solution (see medium 212) 5.00 ml Solution D: Lactose 5.00 g Distilled water 25.00 ml Solution E: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Dissolve ingredients of solution A and sparge under 80% N2 and 20% CO2 gas atmosphere for 30 - 45 min to make it anoxic. Thereafter, adjust pH to 6.4 and dispense under same gas atmosphere in culture vessels and autoclave. Anoxic stock solutions C, D, E and F are prepared separately under 100% N2 gas. Solution B is prepared und 80% N2 and 20% CO2 gas atmosphere. Filter sterilize solution C. Solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the completed medium to 6.4 - 6.8. Note: Rumen fluid may be replaced by supplementing the medium with 200 μg/ml of crude egg yolk phosphatidylcholine (Sigma, type IX-E). © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase peptone or casitone, sodium formate, resazurin, sodium bicarbonate, vitamins, lactose, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 338
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium338.pdf 338. CAMPYLOBACTER RECTUS MEDIUM Beef extract (Difco) 3.00 g Trypticase (BBL) 9.00 g Yeast extract 11.00 g NaCl 2.00 g Na2HPO4 0.40 g Na2CO3 0.25 g Haemin 5.00 mg Na-formate 2.00 g Na-fumarate 3.00 g Resazurin 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of beef extract, trypticase peptone, yeast extract, sodium chloride, sodium phosphate, sodium carbonate, hemin, sodium formate, sodium fumarate, and resazurin. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 342
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium342.pdf 342. METHANOBACTERIUM ALCALIPHILUM MEDIUM Solution A: NH4Cl 1.0 g MgCl2 x 6 H2O 0.1 g CaCl2 x 2 H2O 0.1 g K2HPO4 0.4 g Na2SeO3 x 5 H2O 0.2 mg Trace element solution (see medium 141) 10.0 ml Yeast extract 2.0 g Trypticase (BD BBL) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 780.0 ml Solution B: NaHCO3 10.0 g Na2CO3 0.5 g Distilled water 210.0 ml Solution C: TRIS-HCl buffer 2 M (pH 8.4) 10.0 ml Bring solution A (except cysteine) to the boil, then cool to room temperature under 100% H2 gas. Add cysteine and adjust pH to 7.0 if necessary. Dispense under 100% H2 gas atmosphere in anoxic Hungate-type tubes or serum vials vials (e.g., 3.9 ml per Hungate tube), then autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solution C is prepared under 100% N2 gas atmosphere. Complete the medium by adding appropriate amounts of solutions B and C to the sterile solution A. Adjust pH of completed medium to 8.3 - 8.4, if necessary. After inoculation add sterile H2 gas to 1 - 2 bar overpressure. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium chloride, magnesium chloride, calcium chloride, potassium phosphate, sodium selenite, trace elements, yeast extract, trypticase peptone, resazurin, L-cysteine hydrochloride, sodium bicarbonate, sodium carboante, and tris buffer. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
DSMZ Medium 340
An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, glucose, sodium chloride, potassium nitrate, and hemin. Prepared under an atmosphere of carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium340.pdf 340. CAPNOCYTOPHAGA MEDIUM Trypticase (BBL) 17.0 g Yeast extract 3.0 g Glucose 3.0 g NaCl 3.0 g KNO3 3.0 g Haemin 3.0 mg Distilled water 1000.0 ml Adjust pH to 7.0. Sterilize glucose separately. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 343a
An organic-rich, liquid culture medium comprised of soluble starch, potassium phosphate, trace elements, nickel chloride, sodium chloride, artificial seawater, yeast extract, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium343a.pdf 343a. THERMOSIPHO MELANESIENSIS MEDIUM Starch, soluble 5.0 g KH2PO4 0.5 g Trace element solution (see medium 141) 15.0 ml NiCl2 x 6 H2O 2.0 mg NaCl 20.0 g Artificial sea water (see medium 343) 250.0 ml Yeast extract (Difco) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 750.0 ml Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere, dispense under same gas atmosphere in culture vessels (30% of volume) and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of completed medium and adjust to 6.5, if necessary. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 343
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium343.pdf 343. THERMOTOGA NEAPOLITANA MEDIUM Starch, soluble 5.0 g KH2PO4 0.5 g Trace element solution (see medium 141) 15.0 ml NiCl2 x 6 H2O 2.0 mg Artificial sea water (see below) 250.0 ml Yeast extract (Difco) 2.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 750.0 ml Dissolve ingredients (except sulfide and cysteine) and adjust pH to 6.5. Boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere, dispense under same gas atmosphere in culture vessels (30% of volume) and autoclave. Add sulfide and cysteine from sterile anoxic stock solutions prepared under 100% N2 gas. Check pH of completed medium and adjust to 6.5, if necessary. Artificial sea water: NaCl 27.70 g MgSO4 x 7 H2O 7.00 g MgCl2 x 6 H2O 5.50 g KCl 0.65 g NaBr 0.10 g H3BO3 30.00 mg SrCl2 x 6 H2O 15.00 mg Citric acid 10.00 mg KI 0.05 mg CaCl2 x 2 H2O 2.25 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of soluble starch, potassium phosphate, trace elements, nickel chloride, artificial seawater, yeast extract, resazurin, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 344
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium344.pdf 344. FLEXIBACTER MEDIUM Na-glutamate 5.0 g MgSO4 x 7 H2O 1.0 g Yeast extract (Difco) 1.0 g Glucose (sterilized separately) 2.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic rich, solid culture medium comprised of monosodium glutamate, magnesium sulfate, yeast extract, glucose, and agar.
DSMZ Medium 347
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium347.pdf 347. SYNTROPHOMONAS SAPOVORANS MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BD BBL) 1.00 g PIPES (SIGMA) 15.00 g Resazurin 0.50 mg Distilled water 780.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-laurate 2.78 g Distilled water 25.00 ml Solution E: CaCl2 x 2 H2O 1.84 g Distilled water 10.00 ml Solution F: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution G: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.0, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in anoxic culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust final pH of medium to 7.2. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, clarified rumen fluid, trypticase peptone, PIPES, resazurin, sodium bicarbonate, vitamins, sodium laurate, calcium chloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 346
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium346.pdf 346. DESULFOTOMACULUM SAPOMANDENS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g CaCl2 x 2 H2O 0.15 g MgCl2 x 6 H2O 0.40 g NaCl 1.00 g Trace elements SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg Distilled water 965.00 ml Solution B: NaHCO3 1.00 g Distilled water 20.00 ml Solution C: Ethanol 1.00 ml Distilled water 10.00 ml Solution D: Na-benzoate, 7% w/v 0.70 g Distilled water 10.00 ml Solution E: Rumen fluid, clarified (see medium 1310) 1.00 ml Solution F: Vitamin solution (see medium 141) 10.00 ml Solution G: Na-dithionite 25.00 mg Distilled water 1.00 ml Solution H: Na2S x 9 H2O 50.00 mg Distilled water 1.70 ml Dissolve ingredients of solution A and sparge under 100% N2 gas atmosphere for 30 - 45 min to make it anoxic. Thereafter, dispense solution A under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Anoxic stock solutions C, D, E, F, G and H are prepared separately under 100% N2 gas atmosphere. Filter sterilize solutions D, F and G. Solutions B to H are added to the sterile solution A in the sequence as indicated. Adjust the pH of the completed medium to 7.2 - 7.5. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, resazurin, sodium bicarbonate, ethanol, sodium benzoate, clarified rumen fluid, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 347a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium347a.pdf 347a. SYNTROPHOMONAS ZEHNDERI MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Na2SO4 2.80 g PIPES (SIGMA) 15.00 g Resazurin 0.50 mg Distilled water 810.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-laurate 2.78 g Distilled water 25.00 ml Solution E: CaCl2 x 2 H2O 1.84 g Distilled water 10.00 ml Solution F: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution G: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.0, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E, F and G are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of the solutions B to G are added to solution A in the sequence indicated. Adjust final pH of medium to 7.2. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, sodium sulfate, PIPES, resazurin, sodium bicarbonate, vitamins, sodium laurate, calcium chloride, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 348
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium348.pdf 348. METHANOHALOPHILUS PORTUCALENSIS MEDIUM NaCl 120.00 g MgCl2 x 6 H2O 7.00 g MgSO4 x 7 H2O 6.00 g CaCl2 x 2 H2O 0.50 g K2HPO4 x 3 H2O 0.40 g NH4Cl 1.00 g KCl 3.80 g Trace elements SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Trypticase (BBL) 2.00 g Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g Na2CO3 1.50 g Trimethylamine-HCl 1.90 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients except cysteine, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add cysteine and adjust pH to 6.5, then distribute into anoxic Hungatetype tubes or serum vials under the same gas atmosphere and autoclave. Thereafter, add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Control final pH and adjust to pH 7.3 - 7.4, if necessary. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium phosphate, ammonium chloride, potassium chloride, trace elements, selenite-tungstate, yeast extract, trypticase peptone, resazurin, L-cysteine hydrochloride, sodium carbonate, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 350
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium350.pdf 350. CELLULOMONAS FERMENTANS MEDIUM K2HPO4 2.21 g KH2PO4 1.50 g (NH4)2SO4 1.30 g MgCl2 x 6 H2O 0.10 g CaCl2 x 2 H2O 0.02 g FeSO4 x 7 H2O 1.25 mg Yeast extract 5.00 g Cellobiose 5.00 g or Cellulose (MN 300) 5.00 g NaHCO3 0.80 g Resazurin 1.00 mg Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Adjust pH to 7.4 with 8 M NaOH. Prepare the medium anaerobically under 100% nitrogen. Cellobiose, NaHCO3, and cysteine are sterilized separately by autoclaving under N2. For aerobic growth omit resazurin and cysteine. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, magnesium chloride, calcium chloride, ferrous sulfate, yeast extract, cellobiose or cellulose, sodium bicarbonate, resazurin, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
vitamin solution VA for DSMZ Medium 351
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium351.pdf 351. RHODOBACTER ADRIATICUS MEDIUM Na acetate 0.82 g Yeast extract 0.1 g Na2S x 9H2O 0.06 d K2HPO4 1.0 g CaCl2 x 2 H2O 0.1 g MgCl2 x 6 H2O 0.5 g NH4Cl 1.0 g NaHCO3 3.0 g NaCl 25.0 g Na-ascorbate 0.5 g Trace element solution (SLA) 1.0 ml Distilled water 1000.0 ml Adjust pH to 7.0. The medium may be prepared without the addition of Na acetate, yeast extract and Na2S x 9H2O and may be added to the sterile medium from sterile stock solutions. Prepare the medium under an atmosphere of N2. Dispense into screw capped or crimp top bottles fitted with a rubber septum. Sterilize at 121˚C for 15 minutes in screw-caped bottles. After sterilization add 1.0 ml/l filter-sterilized vitamin solution (VA, see below). Incubate at 30˚C at a light intensity of 1500 lux under anaerobic conditions. Trace element solution (SLA): FeCl2 x 4 H2O 1.8 g CoCl2 x 6 H2O 250.0 mg NiCl2 x 6 H2O 10.0 mg CuCl2 x 2 H2O 10.0 mg MnCl2 x 4 H2O 70.0 mg ZnCl2 100.0 mg H3BO3 500.0 mg Na2MoO4 x 2 H2O 30.0 mg Na2SeO3 x 5 H2O 10.0 mg Distilled water 1000.0 ml Adjust pH to 2.0 - 3.0. Vitamin solution (VA): Biotin 10.0 mg Nicotinic acid amide 35.0 mg Thiamine-HCl x 2 H2O 30.0 mg p-Aminobenzoic acid 20.0 mg Pyridoxal hydrochloride 10.0 mg Ca-pantothenate 10.0 mg Vitamin B12 5.0 mg Distilled water 100.0 ml © 2007 DSMZ GmbH - All rights reserved
A vitamin solution comprised of biotin, nicotinic acid amide (nicotinamide), thiamine hydrochloride, p-aminobenzoic acid (4-aminobenzoic acid), pyridoxal hydrochloride, calcium pantothenate, and vitamin B12 (cobalamin).
trace elements solution SLA for DSMZ Medium 351
DSMZ Medium 351
An organic-rich, liquid culture medium comprised of sodium acetate, yeast extract, sodium sulfide, potassium phosphate, calcium chloride, magnesium chloride, ammonium chloride, sodium bicarbonate, sodium chloride, sodium ascorbate, trace elements and vitamins. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 352
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium352.pdf 352. AZOSPIRILLUM AMAZONENSE MEDIUM K2HPO4 0.200 g KH2PO4 0.600 g CaCl2 x 2 H2O 0.020 g MgSO4 x 7 H2O 0.200 g Na2MoO4 x 2 H2O 0.002 g FeCl3 0.010 g Bromothymol blue (0.5% in 0.2N KOH) 5.000 ml Sucrose 5.000 g Agar 1.750 g Distilled water 1000.000 ml Adjust pH to 6.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of potassium phosphate, calcium chloride, magnesium sulfate, sodium molybdate, ferric trichloride, bromothymol blue, sucrose, and agar.
DSMZ Medium 357
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium357.pdf 357. FLEXIBACTER CANADENSIS MEDIUM MgSO4 x 7 H2O 0.1 g KNO3 0.1 g CaCl2 x 2 H2O 0.1 g Na-glycerophosphate 0.1 g Trace elements SL-10 (see medium 320) 1.0 ml Tris 1.0 g Thiamine-HCl x 2 H2O 1.0 mg Casamino acids 1.0 g Glucose (sterilized separately) 1.0 g Vitamin B12 1.0 μg Agar 10.0 g Distilled water 1000.0 ml Adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 354
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, magnesium citrate, L-asparagine, potato flour, malachite green, glycerol, and homogenized chicken eggs.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium354.pdf 354. LÖWENSTEIN-JENSEN MEDIUM KH2PO4 2.50 g MgSO4 0.24 g Mg-citrate 0.60 g L-Asparagin 3.60 g Potato flour 30.00 g Malachite green 0.40 g Distilled water 600.00 ml Addition: Glycerol 12.00 ml *Fresh egg mixture (yolk and whites) 1000.00 ml pH 7.0 Sterilize medium for 30 min at 121˚C, cool to 30˚C and add fresh egg mixture carefully to the medium. * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 358
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358.pdf 358. ACIDIANUS MEDIUM (AEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 1.00 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature and adjust pH to 2.5 using 10 N H2SO4. Dispense under air atmosphere enriched with 1 - 10% CO2 in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, elemental sulfur, and yeast extract. Prepared under an atmosphere of carbon dioxide.
DSMZ Medium 358a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium358a.pdf 358a. ACIDIANUS MEDIUM (ANAEROBIC) (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Sulfur, powder 5.00 g Yeast extract (Difco) 0.50 g Distilled water 1000.00 ml Dissolve ingredients, bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas mixture and adjust pH to 2.5 using 10 N H2SO4. Dispense under same gas mixture in suitable culture vessels (e.g., 20 ml medium in 100 ml serum bottles) while keeping the sulfur suspended by magnetic stirring. For sterilization sealed bottles with medium are heated in a boiling water bath for 2 - 3 h on each of 3 successive days. Pressurize inoculated bottles to 1 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Inoculate with 5% (w/v) culture. Incubate without shaking. For DSM 3772 reduce amount of yeast extract to 0.02 g/l. For DSM 6296 reduce amount of yeast extract to 0.20 g/l and adjust pH to 2.5 – 3.0. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. For DSM 6334 reduce amount of yeast extract to 0.20 g/l. Pressurize inoculated bottles to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, elemental sulture, and yeast extract. Prepared under an atmosphere of dihydrogen and carbon dioxide.
DSMZ Medium 358a.3
Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased. The overpressure of dihydrogen and carbon dioxide are increased.
DSMZ Medium 358a.2
Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased and the pH is increased to 2.5-3.0. The overpressure of dihydrogen and carbon dioxide is increased.
DSM 6296 is Stygiolobus azoricus DSM 6296
DSMZ Medium 358a.1
Similar to DSMZ Medium 358a, except the concentration of yeast extract is decreased.
DSMZ Medium 359
An organic-rich, solid culture medium comprised of glucose, fructose, yeast extract, calcium carbonate, vitamin B12 (cobalamin), and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium359.pdf 359. GFY MEDIUM Glucose 5.000 g Fructose 5.000 g Yeast extract 5.000 g CaCO3 3.000 g Vitamin B12 0.002 g Agar 15.000 g Distilled water 1000.000 ml © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 360
An organic-rich, solid culture medium comprised of yeast extract, peptone, mannitol, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium360.pdf 360. YPM MEDIUM Yeast extract 5.0 g Peptone 3.0 g Mannitol 25.0 g Agar 12.0 g Distilled water 1000.0 ml pH not adjusted. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 362
Similar to DSMZ Medium 1, except ethanolamine and sodium chloride are added, and the pH is increased to 7.5.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium362.pdf 362. FLAVOBACTERIUM TIRRENICUM MEDIUM To medium 1 add 2 g/l of ethanolamine and 10 g/l NaCl and adjust pH to 7.5. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 364
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium364.pdf 364. PYEA MEDIUM Peptone 10.0 g Yeast extract 10.0 g NaCl 5.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of peptone, yeast extract, sodium chloride, and agar.
DSMZ Medium 363.1
Similar to DSMZ Medium 363, except antipyrin is omitted and L-phenylalanine is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium363.pdf 363. PHENYLOBACTERIUM MEDIUM Antipyrine 1.00 g KH2PO4 0.30 g Na2HPO4 x 12 H2O 0.70 g (NH4)2HPO4 0.70 g NH4H2PO4 0.30 g (NH4)2SO4 0.10 g CaCl2 x 6 H2O 0.05 g MgSO4 x 7 H2O 0.25 g H3BO3 0.50 mg CuSO4 x 5 H2O 0.04 mg KI 0.10 mg FeCl3 x 6 H2O 0.20 mg MnSO4 x 4 H2O 0.40 mg ZnSO4 x 7 H2O 0.40 mg (NH4)2MoO4 0.20 mg Biotin 0.10 mg Vitamin B12 0.03 mg Distilled water 1000.00 ml For DSM 2118, replace antipyrin by 1 g/l L-phenylalanine. © 2007 DSMZ GmbH - All rights reserved
DSM 2118 is not in www.dsmz.de or in TaxBrowser. StrainInof lists this as Phenylobacterium immobile.
DSMZ Medium 363
The pH of this medium should be about 6.9, given the ratio of potassium and sodium phosphates.
An organic-rich, liquid culture medium comprised of antipyrine, potassium phosphate, sodium phosphate, ammonium phosphate, calcium chloride, magnesium sulfate, boric acid, copper sulfate, potassium iodide, ferric trichloride, manganese sulfate, zinc sulfate, ammonium molybdate, biotin, and vitamin B12 (cobalamin).
DSMZ Medium 365
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium365.pdf 365. PSEUDOMONAS INDIGOFERA MEDIUM Yeast extract 10.0 g Glucose 5.0 g Na-acetate 0.5 g Agar 12.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. Rehydrate and grow lyophilized cells in liquid broth and on agar. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, glucose, sodium acetate, and agar.
DSMZ Medium 369
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium369.pdf 369. BACILLUS TUSCIAE MEDIUM Adjust medium 261 to pH 4.0. Incubate at 50˚C without agitation under 5% O2, 10% CO2, 45% H2 (v/v). © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 261, except the pH is decreased to 4.0.
DSMZ Medium 368
An organic-rich, liquid culture medium comprised of tryptone, peptone, yeast extract, glucose, tween 80 (polysorbate 80), filtered tomato juice and ethanol.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium368.pdf 368. KUNKEE MEDIUM Tryptone 20.0 g Peptone 5.0 g Yeast extract 5.0 g Glucose 5.0 g Tween 80 0.5 ml Filtered tomato juice 250.0 ml Distilled water 750.0 ml Adjust pH to 5.5. After sterilization aseptically add 10% (v/v) ethanol © 2007 DSMZ GmbH - All rights reserved
mineral solution for DSMZ Medium 321
DSMZ Medium 311.8
Similar to DSMZ Medium 311, except betaine, cysteine, and sulfide are omitted and sodium lactate and dithiothreitol (1,4-dithiothreitol) are added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium311.pdf 311. SPOROMUSA MEDIUM NH4Cl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.25 g NaCl 2.25 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Selenite-tungstate solution (see medium 385) 1.00 ml Yeast extract 2.00 g Casitone 2.00 g Betaine x H2O 6.70 g Resazurin 0.50 mg K2HPO4 0.35 g KH2PO4 0.23 g NaHCO3 4.00 g Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 1000.00 ml Dissolve ingredients (except phosphates, bicarbonate, vitamins, cysteine and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add phosphates, vitamins (sterilized by filtration), cysteine and sulfide to the medium after autoclaving from sterile stock solutions prepared under 100% N2 gas and bicarbonate from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to pH 7.0. For DSM 3132 and DSM 5090 prepare the medium without betaine; add 5.00 g/l Dfructose to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 4440 use 1.35 g/l betaine; cysteine and sulfide must be replaced by 0.15 g/l DLdithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 6539, DSM 6540, and DSM 17108 prepare the medium without betaine; add 2.00 g/l D-glucose to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 10669, DSM 12793, DSM 12797, and DSM 12993 prepare the medium without betaine; add 5.00 g/l D-fructose to the autoclaved medium from a filter-sterilized anoxic stock solution. Adjust pH of completed medium to 6.5. For DSM 14980 prepare the medium without betaine; add 8.00 g/l D-glucose and 2.00 g/l Na-pyruvate to the autoclaved medium from filter-sterilized anoxic stock solutions. For DSM 16652 prepare the medium without betaine; add 1.11 g/l N-acetyl-D-glucosamine to the autoclaved medium from a filter-sterilized anoxic stock solution. For DSM 17189 and DSM 17285 prepare the medium without betaine; add 3.60 g/l Dmannitol to the autoclaved medium from a filter-sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.30 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. For DSM 26827 prepare the medium without betaine; add 2.50 g/l Na-(DL)-lactate to the autoclaved medium from a separately sterilized anoxic stock solution. Cysteine and sulfide must be replaced by 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved
V-8 juice
Sodium content is 640 mg per 240 mL (= 0.267% Na+, or =0.68% NaCl).
http://www.campbellfoodservice.com/details.aspx?code=61 INGREDIENTS RECONSTITUTED VEGETABLE JUICE BLEND (WATER AND CONCENTRATED JUICES OF TOMATOES, CARROTS, CELERY, BEETS, PARSLEY, LETTUCE, WATERCRESS, SPINACH), CONTAINS LESS THAN 2% OF: SALT, VITAMIN C (ASCORBIC ACID), NATURAL FLAVORING, CITRIC ACID.
An undefined organic chemical mixture comprised of tomato juice (the fruit of Solanum lycopersicum), carrot juice (the root of Daucus carota), celery juice (the stems of Apium graveolens Dulce Group), beet juice (the root of Beta vulgaris), parsley juice (the leaf of Petroselinum crispum), lettuce juice (the leaf of Lactuca sativa), watercress juice (leaf of Nasturtium officinale), and spinach juice (leaf of Spinacia oleracea). Also contains salt (sodium chloride), vitamin C (ascorbic acid), natural flavoring (flavoring agent), and citric acid.
Tryptic Soy Broth without Dextrose
An organic-rich, liquid culture medium comprised of pancreatic digest of casein (casitone), enzymatic digest of soybean meal (soy peptone), sodium chloride, and dipotassium phosphate (potassium dibasic phosphate).
trace elements solution for DSMZ Medium 315
A trace elements solution comprised of nitrilotriacetic acid, magnesium sulfate, manganese sulfate, sodium chloride, ferrous sulfate, cobalt sulfate, calcium chloride, zinc sulfate, copper sulfate, potassium aluminum sulfate, boric acid, sodium molybdate, nickel chloride, and sodium selenite.
DSMZ Medium 311.7
DSMZ Medium 311.7 -< for DSM 17189 and DSM 17285
Similar to DSMZ Medium 311, except betaine, sulfide, and cysteine are omitted and D-mannitol and dithiothreitol are added.
DSM 17189 is Sporomusa intestinalis DSM 17285 is Sporomusa (not in TaxBrowser or StrainInfo)
DSMZ Medium 311.6
Similar to DSMZ Medium 311, except betaine is omitted and N-acetyl-D-glucosamine is added.
DSMZ Medium 311.5
Similar to DSMZ Medium 311, except betaine is omitted and glucose and sodium pyruvate are added.
DSMZ Medium 311.4
Similar to DSMZ Medium 311, except betaine is omitted and fructose is added. The pH is decreased to 6.5.
DSM 10669 is Sporomusa silvacetica DSM 12793 is Thermicanus aegyptius DSM 12793 DSM 12797 is Moorella thermoacetica DSM 12993 is Moorella thermoacetica
DSMZ Medium 311.3
DSM 6539 is Terrisporobacter mayombei DSM 6540 is Acetonema longum DSM 6540 DSM 17108 is Pelosinus fermentans DSM 17108
Similar to DSMZ Medium 311, except betaine, cysteine, and sulfide are omitted and glucose and dithiothreitol are added.
DSMZ Medium 311.3 -< for DSM 6539, DSM 6540, and DSM 17108
DSMZ Medium 311.2
Similar to DSMZ Medium 311, except the concentration of betaine in decreased, sulfide is omitted, and dithiothreitol is added.
DSMZ Medium 311.1
DSM 3132 is Sporomusa acidovorans DSM 5090 is Sporomusa malonica DSM 5090
DSMZ Medium 311.1 -< for DSM 3132 and DSM 5090
Similar to DSMZ Medium 311, except betaine is omitted and D-fructose is added.
DSMZ Medium 311
mineral solution for DSMZ Medium 317
DSMZ Medium 332
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, magnesium chloride, sodium formate, sodium acetate, trypticase peptone, yeast extract, resazurin, L-cysteine hydrochloride, sodium carbonate, and sodium sulfide. Prepared under an atmosphere of dihygrogen, carbon dioxide, and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium332.pdf 332. METHANOGENIUM BOURGENSE MEDIUM NH4Cl 1.0 g K2HPO4 x 3 H2O 0.4 g MgCl2 x 6 H2O 0.1 g Na-formate 5.0 g Na-acetate 1.0 g Trypticase peptone (BD BBL) 1.0 g Yeast extract (OXOID) 1.0 g Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Na2CO3 1.5 g Na2S x 9 H2O 0.2 g Distilled water 1000.0 ml Dissolve ingredients except cysteine, carbonate and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve cysteine, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes and autoclave. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture and sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 6.8 - 7.0, if necessary. Note: After growth has started and the culture is becoming turbid add sterile 80% H2 and 20% CO2 gas mixture to 0.5 - 1 bar overpressure. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 331
An organic-rich, liquid culture medium comprised of calcium chloride, ammonium chloride, manganese chloride, trace elements, vitamins, resazurin, yeast extract, glycine, potassium phosphate, sodium phosphate, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium331.pdf 331. ACETOBACTEROIDES GLYCINOPHILUS MEDIUM CaCl2 x 2 H2O 0.13 g NH4Cl 1.00 g MgCl2 x 6 H2O 0.20 g Trace elements (see medium 144) 10.00 ml Vitamin solution (see medium 141) 5.00 ml Resazurin 1.00 mg Yeast extract 0.50 g Glycine 1.50 g KH2PO4 3.00 g Na2HPO4 5.80 g NaHCO3 0.50 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Adjust pH to 7.2 - 7.4 with NaOH. Prepare the medium anaerobically under 100% nitrogen. Prepare concentrated solutions each of yeast extract, glycine, phosphates, NaHCO3 and Na2S separately and sterilize under nitrogen by autoclaving. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 333
A minerals-salts, solid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, trace elements, potassium tetrathionate, bromocresol purple, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium333.pdf 333. THIOBACILLUS TEPIDARIUS MEDIUM KH2PO4 4.0 g K2HPO4 4.0 g NH4Cl 0.4 g MgSO4 x 7 H2O 0.8 g Trace elements (see below) 10.0 ml K2S4O6 3.0 g Bromocresol purple (saturated aqu. sol.) 2.0 ml Bacto Agar DIFCO (for solid medium) 10.0 g Distilled water 1000.0 ml The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. Adjust pH of final medium to 6.9. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately. Trace element solution: Na2 - EDTA 50.0 g ZnSO4 x 7 H2O 11.0 g CaCl2 x 2 H2O 7.34 g MnCl2 x 4 H2O 2.5 g CoCl2 x 6 H2O 0.5 g (NH4)6 Mo7O24 x 4 H2O 0.5 g FeSO4 x 7 H2O 5.0 g CuSO4 x 5 H2O 0.2 g NaOH ca 11.0 g Distilled water 1000.0 ml First dissolve EDTA in distilled water by adjusting the pH to 7.0 - 8.0 using NaOH; then add other compounds. Adjust pH of final solution to 6.0. © 2007 DSMZ GmbH - All rights reserved
trace elements solution for DSMZ Medium 333
A trace elements solution comprised of disodium EDTA, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, hexaammonium heptamolybdate, ferrous sulfate, copper chloride, and sodium hydroxide.
DSMZ Medium 334.2
Similar to DSMZ Medium 334, except yeast extract and trypticase peptone are added.
DSM 17206 is not in www.dsmz.de. Is Methanosaeta harundinacea 8Ac according to Strain Info.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium334.pdf 334. METHANOTHRIX MEDIUM Solution A: KH2PO4 0.22 g Na2HPO4 x 2 H2O 0.86 g Na-acetate 6.80 g Na2-EDTA 1.00 mg Trace elements solution (see medium 144) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: NaHCO3 4.00 g Distilled water 40.00 ml Solution C: NaCl 0.30 g CaCl2 x 2 H2O 0.11 g MgCl2 x 6 H2O 0.10 g Distilled water 10.00 ml Solution D: NH4Cl 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 141, but 10x conc.) 1.00 ml Solution F: Na2S x 9 H2O 0.50 g Distilled water 10.00 ml Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, dispense under same gas atmosphere in anoxic serum vials (e.g., 20 ml medium in 50 ml serum bottles) and autoclave. Prior to inoculation complete the medium by adding appropriate amounts of sterile solutions C, D, E and F prepared under 100% N2 gas and solution B prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins are sterilized by filtration. Adjust the pH of the complete medium to 7.3 – 7.5. Note: Use 20% (v/v) inoculum. For DSM 3870, DSM 4774 and DSM 6194 supplement medium with 10.00 ml/l of a coenzyme M (2-mercaptoethanesulfonate) solution (1.42% w/v, prepared under N2) and adjust pH of final medium to 7.0. For DSM 17206 supplement medium with 0.50 g/l yeast extract and 0.50 g/l Trypticase peptone added after autoclaving from sterile anoxic stock solutions. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 334.1
Similar to DSMZ Medium 334, except coenzyme M is added, and the pH is decreased to 7.0.
DSM 3870 is Methanosaeta DSM 4774 is Methanosaeta thermophila DSM 6194 is Methanosaeta thermophila PT
DSMZ Medium 334.1 -< for DSM 3870, DSM 4774 and DSM 6194
DSMZ Medium 334
A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium phosphate, sodium acetate, disodium EDTA, trace elements, resazurin, sodium bicarbonate, sodium chloride, calcium chloride, magnesium chloride, ammonium chloride, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 335
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium335.pdf 335. EUBACTERIUM OXIDOREDUCENS MEDIUM Solution A: Mineral solution (see below) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 2.00 g Crotonic acid 5.00 g Vitamin solution (see medium 503) 1.00 ml Resazurin 1.00 mg Distilled water 840.00 ml Adjust pH to 6.9 before autoclaving. Solution B: NaHCO3 5.00 g Distilled water 100.00 ml Solution C: L-Cysteine-HCl x H2O 0.24 g Distilled water 10.00 ml Solution D: Na2S x 9 H2O 78.00 mg Distilled water 1.00 ml Prepare solution A under 80% N2 and 20% CO2 gas mixture, distribute 8.9 ml per Hungate-type tube under same gas atmosphere and autoclave. Filter sterilize NaHCO3 solution and gas for 20 min with 80% N2 and 20% CO2 gas mixture. Autoclave cysteine and sulfide under 100% N2 gas. Complete medium by adding the solutions B, C, and D with a syringe to solution A. Adjust final pH of medium to 7.0 - 7.5. Mineral solution: KH2PO4 10.0 g MgCl2 x 6 H2O 6.6 g NaCl 8.0 g NH4Cl 8.0 g CaCl2 x 2 H2O 1.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, yeast extract, crotonic acid, vitamins, resazurin, sodium bicarbonate, L-cysteine hydrochlrodie, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
mineral solution for DSMZ Medium 335
DSMZ Medium 339.3
Similar to DSMZ Medium 339, except dithiothreitol is added and the pH is increased to 7.4.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339.pdf 339. WILKINS-CHALGREN ANAEROBE BROTH Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving check pH and adjust to pH 6.8, if necessary. For DSM 5676 supplement medium with 10.0 g/l D-glucose. For DSM 12679 and DSM 12858 use medium at half-strength (16.5 g/l). For DSM 23669 supplement medium after autoclaving with 0.2 g/l dithiothreitol (DTT) added from a filter-sterilized anoxic stock solution and adjust pH of completed medium to 7.4 © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 339.2
DSMZ Medium 339.2 -< for DSM 12679 and DSM 12858
DSM 12679 is Caloramator coolhaasii DSM 12858 is Soehngenia saccharolytica DSM 12858
Similar to DSMZ Medium 339, except the medium is diluted.
DSMZ Medium 339.1
DSM 5676 is [Clostridium] scindens ATCC 35704
Similar to DSMZ Medium 339, except the concentration of glucose is increased.
DSMZ Medium 339
Wilkins-Chalgren Anaerobe Broth (N2/CO2)
An organic-rich, liquid culture medium comprised of Wilkins-Chalgren medium, resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 339a.4
DSMZ Medium 339a.4 -< for DSM 15243 and DSM 15248
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium339a.pdf 339a. WILKINS-CHALGREN ANAEROBE BROTH (N2/CO2) Dissolve dehydrated Wilkins-Chalgren medium (Oxoid CM0643) and resazurin (0.5 mg/l) in distilled water, bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Add L-cysteine-HCL x H2O (0.3 g/l), dispense the medium in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving supplement medium with 5.0 g/l D-glucose added from a sterile anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.8 with a sterile anoxic stock solution of Na2CO3 (5% w/v) prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 14428 replace D-glucose with 5.0 g/l cellobiose prepared under 100% N2 gas atmosphere and sterilized by filtration. For DSM 14924 adjust final pH of medium to 7.4 using a sterile anoxic stock solution of Na2CO3 prepared under 80% N2 and 20% CO2 gas atmosphere. For DSM 15176, DSM 15480, DSM 15481 and DSM 15567 omit D-glucose. For DSM 15243 and DSM 15248 omit Na2CO3 and adjust final pH of medium to 6.0. © 2014 DSMZ GmbH - All rights reserved
DSM 15243 is Clostridium carboxidivorans P7 DSM 15248 is Clostridium ragsdalei P11
Similar to DSMZ Medium 339a, except the final pH is decreased to 6.0.
DSMZ Medium 339a.3
DSM 15176 is Subdoligranulum variabile DSM 15176 DSM 15480 is Hespellia stercorisuis DSM 15480 DSM 15481 is Hespellia porcina DSM 15567 is Mahella australiensis 50-1 BON
DSMZ Medium 339a.3 -< for DSM 15176, DSM 15480, DSM 15481 and DSM 15567
Similar to DSMZ Medium 339a, except D-glucose is omitted.
DSMZ Medium 339a.2
Similar to DSMZ Medium 339a, except the pH is increased to 7.4.
DSMZ Medium 339a.1
Similar to DSMZ Medium 339a, except D-glucose is omitted and cellobiose added.
DSMZ Medium 339a
An organic-rich, liquid culture medium comprised of Wilkins-Chalgren medium, resazurin, L-cysteine hydrochloride, and D-glucose. Prepared under an atmosphere of dinitrogen and carbon dioxide.
potato flour
Flour made from ground, dried potato tubers.
DSMZ Medium 383.13
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383.pdf 383. DESULFOBACTERIUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Substrate (see below) Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. Substrates to be used and additional instructions: see next page! DSM 2056: Solution D: Na-butyrate 0.70 g Na-caproate 0.30 g Na-octanoate 0.15 g Distilled water 10.00 ml DSM 3383: Dissolve 0.3 g of indole in 100 ml water by heating and shaking in a closed bottle under nitrogen gas, autoclave, then add sterile anaerobic 30% NaCl solution (7 ml) and 40% MgCl2 x 6 H2O solution (0.7 ml). Store the indole solution in the dark. Reheat and shake before use. Add 30 ml/l medium in the beginning, 2 x 30 ml/l during growth. DSM 3384: Solution D: Na-benzoate 0.40 g Phenol 0.04 g Distilled water 10.00 ml DSM 4661: Solution D: Resorcinol 0.11 g Distilled water 10.00 ml During growth the culture is fed once with the same amount of resorcinol. DSM 5091 and DSM 9788: Solution D: Malonic acid 2.00 g Distilled water 10.00 ml DSM 7044, DSM 7120, DSM 7467, DSM 12567, and DSM 13228: Solution D: Na-benzoate 0.40 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) and 0.1 g/l of yeast extract added to the autoclaved medium from anoxic stock solutions sterilized by filtration. DSM 8540: Solution D: Na-4-hydroxybenzoate 0.30 g Distilled water 10.00 ml DSM 8541, DSM 10085 and DSM 16918: Solution D: Na-pyruvate 2.50 g Distilled water 10.00 ml DSM 9705: Solution D: Na-glycolate 1.00 g Distilled water 10.00 ml Sterilize by filtration! DSM 12861 and DSM 12883: Solution D: Valeric acid 1.00 g Distilled water 10.00 ml DSM 12888: Solution D: Na-butyrate 1.10 g Caproic acid 1.10 g Distilled water 10.00 ml DSM 13036: Solution D: Betaine 1.00 g Distilled water 10.00 ml DSM 14454: Solution D: Naphthalene 0.40 g Heptamethylnonane 20.00 ml Alternatively: Na-pyruvate 1.10 g Distilled water 10.00 ml DSM 15576 and DSM 16219: Solution D: Na-caprylate 1.00 g Distilled water 10.00 ml Supplement medium with 1 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. DSM 17291: Solution D: Casamino acids (BD BBL) 3.00 g Distilled water 20.00 ml DSM 17477: Solution D: Sodium glutamate x H2O 1.90 g Yeast extract 1.00 g Distilled water 20.00 ml DSM 28890: Solution D: Sodium formate 0.68 g Yeast extract 0.50 g Distilled water 20.00 ml © 2015 DSMZ GmbH - All rights reserved
DSM 14454 is delta proteobacterium NaphS2
Similar to DSMZ Medium 383, except naphthalene and heptamethylnonane (isocetane) are added (or sodium pyruvate is added).
DSMZ Medium 383.12
Similar to DSMZ Medium 383, except betaine (glycine betaine) is added.
DSMZ Medium 383.11
Similar to DSMZ Medium 383, except sodium buytrate and caproic acid (hexanoic acid) is added.
DSMZ Medium 383.10
DSM 12861 is delta proteobacterium S2550 DSM 12883 is delta proteobacterium S2551
DSMZ Medium 383.10 -< for DSM 12861 and DSM 12883
Similar to DSMZ Medium 383, except veralic acid is added.
DSMZ Medium 383.9
DSM 9705 is Desulfofustis glycolicus DSM 9705
Similar to DSMZ Medium 383, except sodium glycolate is added.
DSMZ Medium 383.8
DSM 8541 is Desulfococcus sp. DSM 8541 DSM 10085 is Desulfospira joergensenii DSM 10085 DSM 16918 is Desulfovibrio sp. M2
DSMZ Medium 383.8 -< for DSM 8541, DSM 10085 and DSM 16918
Similar to DSMZ Medium 383, except sodium pyruvate is added.
DSMZ Medium 383.7
Similar to DSMZ Medium 383, except sodium 4-hydroxybenzoate is added.
DSMZ Medium 383.6
DSM 7044 is Desulfotignum balticum DSM 7044 DSM 7120 is Desulfobacterium DSM 7467 is Desulfobacula toluolica Tol2 DSM 12567 is sulfate-reducing bacterium mXyS1 DSM 13228 is Desulfosarcina ovata
Similar to DSMZ Medium 383, except sodium benzoate, seven vitamins solution, and yeast extract are added.
DSMZ Medium 383.5
DSM 5091 is Malonomonas rubra DSM 5091 DSM 9788 is Malonomonas rubra
Similar to DSMZ Medium 383, except malonic acid is added.
DSMZ Medium 383.5 -< for DSM 5091 and DSM 9788
DSMZ Medium 383.4
DSM 4661 is an unclassified bacterium with no sequences.
Similar to DSMZ Medium 383, except resorcinol is added.
DSMZ Medium 383.3
Similar to DSMZ Medium 383, except sodium benzoate and phenol are added.
DSMZ Medium 383.2
Similar to DSMZ Medium 383, except indole is added. The concentrations of sodium chloride and magnesium dichloride are increased.
DSMZ Medium 383.1
Similar to DSMZ Medium 383, except sodium butyrate, sodium caproate, and sodium octanoate are added.
DSMZ Medium 383
A mineral-salts, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, vitamins, sodium dithionite and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 383a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383a.pdf 383a. DESULFOTIGNUM MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 15.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 1.00 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-pyruvate 1.0 g Distilled water 50.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solutions D and E are prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium pyruvate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 383b.3
DSMZ Medium 383b.3 -< for DSM 15286 and DSM 21156
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium383b.pdf 383b. DESULFONAUTICUS MEDIUM Solution A: Na2SO4 3.00 g KH2PO4 0.20 g NH4Cl 0.30 g NaCl 21.00 g MgCl2 x 6 H2O 3.00 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Selenite-tungstate solution (see medium 385) 1.00 ml Resazurin 0.50 mg Distilled water 930.00 ml Solution B: Trace elements SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate 1.00 g Distilled water 10.00 ml Solution E: Vitamin solution (see medium 141) 10.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed in anoxic cultivation vials and autoclaved under the same gas atmosphere. Solutions B, D and F are autoclaved separately under 100% N2 gas. Solution C is autoclaved under 80% N2 and 20% CO2 gas atmosphere. Solution E is prepared under 100% N2 gas and filter-sterilized. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.0 – 7.2. After inoculation pressurize vials to 2 bar overpressure with 80% H2 and 20% CO2 gas mixture. Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% inoculum. Incubate all strains in the dark. For DSM 3382 omit Na-acetate. For DSM 4206 and DSM 15269 supplement medium with 2.00 g/l Trypticase peptone and 2.00 g/l yeast extract added to the autoclaved medium from sterile anoxic stock solutions prepared under N2 gas. For DSM 15286 and DSM 21156 supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of medium to 6.5 – 6.7 prior to inoculation. © 2015 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 383b, except seven vitamins solution added and the pH is decreased to 6.5-6.7.
DSM 15286 is Thermodesulfatator indicus DSM 15286 DSM 21156 is Thermodesulfatator atlanticus DSM 21156
DSMZ Medium 383b.2
DSM 4206 is Desulfonauticus autotrophicus DSM 4206 DSM 15269 is Desulfonauticus submarinus
DSMZ Medium 383b.2 -< for DSM 4206 and DSM 15269
Similar to DSMZ Medium 383b, except seven vitamins solution is added. The pH is decreased to 6.5-6.7.
DSMZ Medium 383b.1
DSM 3382 is Desulfobacterium autotrophicum HRM2
Similar to DSMZ Medium 383b, sodium acetate is omitted.
DSMZ Medium 383b
An organic-rich, liquid culture medium comprised of sodium sulfate, potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, selenite-tungstate, resazurin, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium dithionite, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
DSMZ Medium 386.1
Similar to DSMZ Medium 386, except sodium pyrosulfite is omitted and sodium thiosulfate is added.
DSM 7269 is Desulfocapsa thiozymogenes DSM 7269
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium386.pdf 386. DESULFOVIBRIO SULFODISMUTANS MEDIUM Solution A: KH2PO4 0.20 g NH4Cl 0.30 g NaCl 1.00 g MgCl2 x 6 H2O 0.40 g KCl 0.50 g CaCl2 x 2 H2O 0.15 g Distilled water 920.00 ml Solution B: Trace element solution SL-10 (see medium 320) 1.00 ml Solution C: NaHCO3 2.50 g Distilled water 50.00 ml Solution D: Na-acetate x 3 H2O 0.30 g Distilled water 10.00 ml Solution E: Vitamins solution (see medium 503) 1.00 ml Solution F: Na2S x 9 H2O 0.40 g Distilled water 10.00 ml Solution G: Na2S2O5 (= sodium pyrosulfite) 0.95 g Distilled water 10.00 ml Adjust pH to 7.5-8.0 with NaOH. Solution A is sparged 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then distributed in anoxic Hungate-type tubes or serum vials under the same gas mixture and autoclaved. Solutions B, D and F are sparged with 100% N2 gas and autoclaved separately. Solution C is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Solutions E and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of the solutions B to G are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be at 7.1 - 7.4. Note: When growth has started feed culture again with same amount of pyrosulfite. After a further two days repeat feeding once more. For DSM 7269 replace solution G with 2.50 g/l Na2S2O3 x 5 H2O added to the medium after autoclaving from a sterile anoxic stock solution. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 386
A minerals-salts, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium chloride, magnesium chloride, potassium chloride, calcium chloride, trace elements, sodium bicarbonate, sodium acetate, vitamins, sodium sulfide, and sodium pyrosulfite (sodium disulfite). Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 388
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium chloride, calcium chloride, ferrous ammonium sulfate, cobalt chloride, sodium molybdate, disodium selenite, manganese chloride, zinc sulfate, yeast extract, polypeptone, soluble starch, vitamins, resazurin, sodium carbonate, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium388.pdf 388. DICTYOGLOMUS MEDIUM KH2PO4 1.50 g Na2HPO4 x 12 H2O 4.20 g NH4Cl 0.50 g MgCl2 x 6 H2O 0.38 g CaCl2 x 2 H2O 0.06 g Fe(NH4)2(SO4)2 x 6 H2O 0.04 g CoCl2 x 6 H2O 2.90 mg Na2MoO4 x 2 H2O 2.40 mg Na2SeO3 x 5 H2O 0.17 mg MnCl2 x 4 H2O 2.00 mg ZnSO4 2.80 mg Yeast extract 2.00 g Polypeptone 2.00 g Starch, soluble 5.00 g Vitamin solution (see medium 141) 10.00 ml Resazurin 0.50 mg Na2CO3 1.00 g L-Cysteine-HCl x H2O 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except carbonate and cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas. Add and dissolve carbonate and cysteine, adjust pH to 7.2, then dispense under same gas atmosphere in culture vessels and autoclave. Adjust pH of final medium to 7.2. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 389
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium chloride, magnesium sulfate, agar, trace elements, and sodium thiosulfate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium389.pdf 389. THIOBACILLUS AQUAESULIS MEDIUM Solution A: Na2HPO4 x 2 H2O 7.9 g KH2PO4 1.5 g Distilled water 100.0 ml Adjust to pH 7.6. Solution B: NH4Cl 0.4 g MgSO4 x 7 H2O 0.1 g Agar (for solid medium) 15.0 g Distilled water 870.0 ml Solution C: Trace elements (see medium 333) 10.0 ml Solution D: Na2S2O3 x 5 H2O 5.0 g Distilled water 20.0 ml Autoclave solutions A, B and C and filter sterilize solution D. To complete the medium combine sterile solutions when cool. © 2012 DSMZ GmbH - All rights reserved
TyC solution for DSMZ Medium 391
An undefined organic chemical mixture comprised of L-tryptophan, casamino acids, and yeast extract. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium391.pdf 391. METHANOCOCCUS VOLTAE MEDIUM Mineral salt solution 2xW (see below) 500.0 ml Trace element solution (see medium 141) 10.0 ml LIP-solution (see below) 50.0 ml Na-acetate 1.0 g Resazurin 0.5 mg TYC-solution (see below) 50.0 ml NaHCO3 5.0 g L-Cysteine-HCl x H2O 0.5 g Na2S x 9 H2O 0.5 g Distilled water 400.0 ml Dissolve ingredients except TYC solution, bicarbonate, cystein and sulfide. Sparge medium with 80% H2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Before use add TYC solution, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.2. Mineral solution 2xW: NaCl 40.00 g MgCl2 x 6 H2O 5.60 g MgSO4 x 7 H2O 0.70 g KCl 0.68 g NH4Cl 0.50 g K2HPO4 0.28 g CaCl2 x 2 H2O 0.28 g Distilled water 1000.00 ml May be stored at room temperature in the dark. LIP-solution: L-Leucine 5.0 g L-Isoleucine 10.0 g Pantothenate 0.1 g Distilled water 1000.0 ml May be stored frozen without sterilization. TYC-solution: Casamino acids 100.0 g Yeast extract 50.0 g L-Tryptophan 1.0 g Distilled water 1000.0 ml Prepare and autoclave under 100% N2 gas. For strain DSM 4254 supplement medium with 80.0 mg/l L-histidine added from a sterile anoxic stock solution sterilized by filtration. For strain DSM 4310 add 32.0 mg/l of hypoxanthine to the medium before autoclaving; supplement medium with 1.0 ml/l of a vitamins solution (see medium 503) added from a sterile anoxic stock solution sterilized by filtration. © 2010 DSMZ GmbH - All rights reserved
LIP solution for DSMZ Medium 391
A defined organic chemical mixture containing L-leucine, L-sioleucine, and calcium pantothenate.
mineral solution 2xW for DSMZ Medium 391
An inorganic salts solution comprised of sodium chloride, magnesium chloride, magnesium sulfate, potassium chloride, ammonium chloride, potassium phosphate, and calcium chloride.
DSMZ Medium 391.2
Similar to DSMZ Medium 391, except hypoxanthine and seven vitamin solution are added.
DSMZ Medium 391.1
Similar to DSMZ Medium 391, except histidine is added.
DSMZ Medium 391
An organic-rich, liquid culture medium comprised of mineral solution 2xW, trace elements, LIP solution, resazurin, TYC solution, sodium bicarbonate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
artificial sea water for DSMZ Medium 343
An artificial seawater comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, citric acid, potassium iodide, and calcium chloride.
DSMZ Medium 465
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465.pdf 465. MINERAL MEDIUM PH 7.25 Na2HPO4 x 2 H2O 3.50 g KH2PO4 1.00 g (NH4)2SO4 0.50 g MgCl2 x 6 H2O 0.10 g Ca(NO3)2 x 4 H2O 0.05 g Trace element solution SL-4 (see below) 1.00 ml Distilled water 1000.00 ml pH 7.25 Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220, 535 or 830). After this reactivation, cultivate on mineral medium with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved
DSMZ Medium 383.14
DSM 15576 is Desulfatibacillum aliphaticivorans DSM 15576 DSM 16219 is Desulfatibacillum alkenivorans DSM 16219
DSMZ Medium 383.14 -< for DSM 15576 and DSM 16219
Similar to DSMZ Medium 383, except sodium caprylate (sodium octanoate) and seven vitamins solution are added.
DSMZ Medium 383.15
Similar to DSMZ Medium 383, except casamino acids are added.
DSM 17291 is Thermovirga lienii DSM 17291
DSMZ Medium 383.16
Similar to DSMZ Medium 383, except sodium glutamate (monosodium glutamate) and yeast extract are added.
DSM 17477 is Dethiosulfatibacter aminovorans DSM 17477
DSMZ Medium 381a
An organic-rich, liquid culture medium comprised of peptone, yeast extract, and sea salt.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381a.pdf 381a. MODIFIED LB Peptone 10.0 g Yeast Extract 5.0 g Sea Salt 25.0 g Distilled water 1000.0 ml Adjust to pH 7.2 and sterilize by autoclaving. The medium may be solidified by adding 12.0 g/l agar. © 2012 DSMZ GmbH - All rights reserved
DSMZ Medium 382
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium382.pdf 382. MINERAL MEDIUM M9 for E. coli JM strains 10 x M9 salts (see below) 100.0 ml 1 M MgSO4 1.0 ml 0.1 M CaCl2 1.0 ml 1 M Thiamine-HCl x 2 H2O (filter-sterilized) 1.0 ml Glucose (20%) 10.0 ml Proline 20.0 mg Distilled water 900.0 ml The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. 10 x M9 salts (per l): Na2HPO4 60.0 g KH2PO4 30.0 g NH4Cl 10.0 g NaCl 5.0 g Adjust pH to 7.4. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of 10 x M9 salts, magnesium sulfate, calcium chloride, thiamine hydrochloride, glucose, and proline.
10 x M9 salts solution for DSMZ Medium 382
An inorganic salts solution comprised of sodium phosphate, potassium phosphate, ammonium chloride, and sodium chloride.
DSMZ MEdium 381
An organic-rich, solid culture medium comprised of tryptone, yeast extract, sodium chloride, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium381.pdf 381. LB (Luria-Bertani) MEDIUM Tryptone 10.0 g Yeast extract 5.0 g NaCl 10.0 g Agar 20.0 g Distilled water 1000.0 ml Adjust pH to 7.0. For DSM 26804 add 100g NaCl (10%) for 1000 ml medium. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 381.1
DSM 26804 is not in www.dsmz.de or in TaxBrower. However there is a publication that lists this as the type strain for Marinobacter piscensis (Curr Microbiol 70(4):544 2015). TaxBrowser lists this species as Marinobacter sp. Abdou3.
Similar to DSMZ Medium 381, except the concentration of sodium chloride is increased.
DSMZ Medium 379
An organic-rich, liquid culture medium comprised of disodium EDTA, magnesium sulfate, calcium chloride, ammonium chloride, sodium chloride, ammonium acetate or sodium pyruvate, potassium phosphate, sodium bicarbonate, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium379.pdf 379. CHROMATIUM TEPIDUM MEDIUM Na2-EDTA 10.0 mg MgSO4 x 7 H2O 200.0 mg CaCl2 x 2 H2O 50.0 mg NH4Cl 400.0 mg NaCl 400.0 mg (NH4) acetate or Na-pyruvate 0.5 mg KH2PO4 0.5 mg NaHCO3 2.0 g Na2S x 9 H2O 1.0 g Trace element solution (see below) 1.0 ml Deionized water 1000.0 ml Prepare bicarbonate and sulfide as separate solutions. Sterilize all solutions by autoclaving. After cooling mix all three solutions, adding the sulfide last. Adjust pH to 7.0 and dispense in completely filled screw-capped tubes or bottles. Incubate at 500 - 1000 Lux light intensity. Trace element solution: Na2-EDTA 5.2 g FeCl2 x 4 H2O 1.5 g ZnCl2 70.0 mg MnCl2 x 4 H2O 100.0 mg H3BO3 6.0 mg CoCl2 x 6 H2O 190.0 mg CuCl2 x 2 H2O 2.0 mg NiCl2 x 6 H2O 25.0 mg Na2MoO4 x 2 H2O 188.0 mg VOSO4 x 2 H2O 30.0 mg Na2WO4 x 2 H2O 2.0 mg Deionized water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
trace elements solution for DSMZ Medium 379
A trace elements solution comprised of disodium EDTA, ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, sodium molybdate, vanadyl sulfate, and sodium tungstate.
DSMZ Medium 380
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium380.pdf 380. MAGNETOSPIRILLUM MEDIUM KH2PO4 0.68 g NaNO3 0.12 g L(+)-Tartaric acid 0.37 g Succinic acid 0.37 g Na-acetate 0.05 g Vitamin solution (see medium 141) 10.00 ml Trace elements (see medium 141) 5.00 ml Fe(III) quinate solution (see below) 2.00 ml Agar (for semi-solid medium) 1.30 g Resazurin 0.50 mg Na-thioglycolate 0.05 g Distilled water 1000.00 ml Dissolve ingredients (except thioglycolate) in the order given and adjust pH to 6.75 with NaOH. Preparation of liquid medium: Purge medium with 100% N2 gas for 30 -45 min and dispense under the same gas atmosphere in anoxic vials to 30% of their volume. Seal vials with screw caps and gas tight rubber closures. Autoclave at 121˚C for 15 min. Before inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to 1% O2 concentration in the gas phase. Preparation of semi solid medium: Supplement medium with agar, bring medium to the boil and cool under 100% N2 gas atmosphere. Dispense under same gas atmosphere aliquots of 10 ml semi-solid medium into 16 x 150 mm Hungate-type tubes. After autoclaving, tubes may be stored at room temperature. Prior to inoculation add thioglycolate from a 3% (w/v) solution, freshly prepared under 100% N2 gas and filter-sterilized. Then add sterile air (with hypodermic syringe through the rubber closure) to a concentration of 1% (v/v) in the vial. Note: Prior to inoculation media should be slightly pink in color. Strongly reduced conditions will not support growth of the organism. Incubate tubes with semi-solid medium without agitation in an upright position. During growth O2 will be consumed, resazurin decolorized and the pH increase. Feed oxygen (by adding air) and succinic acid from sterile 0.05 M solution (to maintain pH below 7). If higher densities of magnetic cell are wanted, ferric quinate also has to be fed. Ferric Quinate Solution, 0.01 M : FeCl3 x 6 H2O 0.45 g Quinic acid 0.19 g Distilled water 100.00 ml Sterilize by filtration under 100% N2 gas atmosphere. For DSM 6361 increase the amount of added O2 to a concentration of 10% (v/v) in the headspace gas atmosphere. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of potassium phosphate, sodium nitrate, tartaric acid, succinic acid, sodium acetate, vitamins, trace elements, ferric quinate solution, agar, resazurin, and sodium thioglycolate. Prepared under an atmosphere of dinitrogen and dioxygen.
DSMZ Medium 380.1
Similar to DSMZ Medium 380, except the concentration of dioxygen is increased.
DSM 6361 is Magnetospirillum gryphiswaldense MSR-1
ferric quinate solution for DSMZ Medium 380
A chemical mixture, comprised of ferric trichloride and quinic acid. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 399
An organic-rich, liquid culture comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, yeast extract, sodium lactate, ferrous ammonium sulfate, trace elements, resazurin, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen, carbon dioxide, and dihydrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium399.pdf 399. ARCHAEOGLOBUS MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Yeast extract (Difco) 0.50 g Na-L-Lactate 1.50 g Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg Trace element solution (see medium 141) 10.00 ml Resazurin 0.50 mg NaHCO3 3.00 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except bicarbonate and sulfide, bring to the boil, then cool quickly to room temperature while gassing with 80% N2 and 20% CO2 gas mixture, add sodium bicarbonate and adjust pH to 6.9. Distribute medium under same gas atmosphere in serum bottles (20 ml per 100 ml bottle), seal, then pressurize bottles up to 2 bar overpressure with 80% N2 and 20% CO2 gas mixture and autoclave. Before use, release excess pressure and reduce the medium with sodium sulfide from a sterile anoxic stock solution prepared under 100% N2 gas. After inoculation, add 2 bar overpressure of 80% H2 and 20% CO2 gas mixture. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 411
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium411.pdf 411. CLOSTRIDIUM ACETOBUTYLICUM MEDIUM Fresh potatoes, washed, peeled and sliced 200.0 g CaCO3 2.0 g Resazurin 0.5 mg D-Glucose 6.0 g L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Mix potatoes and CaCO3 then autoclave 30 min at 121˚C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere in anoxic Hungatetype tubes or serum vials and autoclave 20 min at 121˚C. Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium: Milk Medium: Fresh milk 100.0 ml Resazurin 0.1 mg Adjust pH to 7.1. Tube and autoclave under 100% N2 gas atmosphere for 12 min at 121˚C. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potato infusion, calcium carbonate, resazurin, D-glucose, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 378
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium378.pdf 378. ARTHROBACTER MEDIUM K2HPO4 1.770 g KH2PO4 0.680 g NaCl 0.140 g CaCl2 0.132 g MgSO4 x 7 H2O 0.200 g Mannitol 10.000 g Yeast extract 0.080 g FeSO4 x 7 H2O 2.500 mg H3BO3 2.900 mg CoSO4 x 7 H2O 1.200 mg CuSO4 x 5 H2O 0.100 mg MnCl2 x 4 H2O 0.090 mg Na2MoO4 x 2 H2O 2.500 mg ZnSO4 x 7 H2O 1.200 mg Distilled water 1000.000 ml Adjust pH to 7.0. Incubate under 5% O2 + 95% N2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium chloride, calcium chloride, magnesium sulfate, mannitol, yeast extract, ferrous sulfate, boric acid, cobalt sulfate, copper sulfate, manganese chloride, sodium molybdate, and zinc sulfate. Prepared under an atmosphere of dioxygen and dinitrogen.
DSMZ Medium 377a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377a.pdf 377a. PALAEOCOCCUS MEDIUM NaCl 30.00 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Peptone 5.00 g Yeast extract 1.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without vitamins and sulfide, bring medium to the boil and then cool under a stream of 100% N2 gas. Add sulfide, adjust the pH to 6.5, and distribute in appropriate vessels under 100% N2 gas atmosphere, thereby taking care to transfer also the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour on 3 successive days. Do not autoclave! Prior to inoculation add vitamins from an anoxic stock solution sterilized by filtration. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, peptone, yeast extract, elemental sulfur, resazurin, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
DSMZ Medium 377
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium sulfate, magnesium chloride, potassium chloride, sodium bromide, boric acid, strontium chloride, ammonium sulfate, citric acid, potassium iodide, calcium chloride, potassium phosphate, nickel chloride, trace elements, peptone, elemental sulfur, yeast extract, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium377.pdf 377. PYROCOCCUS MEDIUM NaCl 13.85 g MgSO4 x 7 H2O 3.50 g MgCl2 x 6 H2O 2.75 g KCl 0.33 g NaBr 0.05 g H3BO3 15.00 mg SrCl2 x 6 H2O 7.50 mg (NH4)2SO4 10.00 mg Citric acid 5.00 mg KI 0.05 mg CaCl2 x 2 H2O 0.75 g KH2PO4 0.50 g NiCl2 x 6 H2O 2.00 mg Trace elements (see medium 141) 10.00 ml Peptone 5.00 g Yeast extract 1.00 g Sulfur, powdered 30.00 g Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Prepare the medium without sulfide, bring medium to the boil and then cool under a stream of 100% N2 gas. Add sulfide, adjust the pH to 6.5, and distribute in appropriate vessels under 100% N2 gas atmosphere, thereby taking care to transfer the necessary amount of sulfur. Heat the vessels containing the medium in boiling water for 1 hour on 3 successive days. Do not autoclave! © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 376
An organic-rich, liquid culture medium comprised of trypticase peptone, potassium phosphate, sodium chloride, sodium thioglycolate, elemental sulfur, and resazurin. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium376.pdf 376. AN1 MEDIUM Trypticase (BBL) 10.0 g K2HPO4 1.5 g NaCl 2.5 g Na-thioglycolate 1.0 g Sulfur, powdered 8.0 g Resazurin 1.0 mg Distilled water 1000.0 ml Adjust pH to 7.3. Prepare the medium anaerobically under 100% nitrogen. Sterilize thioglycolate and sulfur separately. Sterilize the sulfur by steaming for 3 h on each of three successive days. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 374
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, ammonium chloride, calcium chloride, ferrous sulfate, trace elements, yeast extract, sodium acetate, sodium formate, sludge fluid, fatty acid mixture, resazurin, sodium bicarbonate, isopropyl alcohol (propan-2-ol), and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium374.pdf 374. METHANOSPIRILLUM SK MEDIUM KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O 2.00 mg Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 1.00 g Na-acetate 1.00 g Na-formate 2.00 g Sludge fluid (see medium 119) 50.00 ml Fatty acid mixture (see medium 119) 20.00 ml Resazurin 0.50 mg NaHCO3 4.00 g Isopropyl alcohol 7.50 ml Na2S x 9 H2O 0.50 g Distilled water 940.00 ml Dissolve ingredients except bicarbonate, isopropyl alcohol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 – 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add isopropyl alcohol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of completed medium and adjust to 7.0 – 7.2, if necessary. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 375
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium375.pdf 375. METHANOHALOBIUM MEDIUM NaCl 250.00 g NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.33 g MgCl2 x 6 H2O 0.33 g MgSO4 x 7 H2O 4.00 g Resazurin 0.50 mg Na2CO3 2.50 g Trace element solution SL-10 (see medium 320) 1.00 ml Yeast extract 0.05 g Trimethylamine-HCl 5.00 g Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate, trace elements, yeast extract, trimethylamine and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for at least 30 – 45 min to make it anoxic, then dispense under same gas atmosphere in anoxic Hungatetype tubes or serum vials and autoclave. Add trace elements, yeast extract, trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of completed medium to 7.4 with an anoxic sterile solution of 5% w/v Na2CO3, if necessary. Note: A white precipitate that forms after autoclaving of the mineral base will dissolve again after storage at room temperature for 2 – 3 days. © 2015 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of sodium chloride, ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, magnesium sulfate, resazurin, sodium carbonate, trace elements, yeast extract, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 373
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium373.pdf 373. METHANOGENIUM CV MEDIUM KCl 0.34 g MgCl2 x 6 H2O 4.00 g MgSO4 x 7 H2O 3.45 g NH4Cl 0.25 g CaCl2 x 2 H2O 0.14 g K2HPO4 0.14 g NaCl 18.00 g Trace elements (see medium 141) 10.00 ml Fe(NH4)2(SO4)2 x 6 H2O 2.00 mg Na-acetate 1.00 g Yeast extract (Oxoid) 2.00 g Trypticase peptone (BD BBL) 2.00 g Resazurin 0.50 mg NaHCO3 5.00 g Isopropyl alcohol 7.50 ml Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except bicarbonate, isopropyl alcohol, vitamins and sulfide), bring medium to the boil, then cool to room temperature under 80% N2 and 20% CO2 gas mixture. Add and dissolve bicarbonate and adjust pH to 7.0, then dispense under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add isopropyl alcohol and cysteine from sterile anoxic stock solutions autoclaved under 100% N2 gas atmosphere. Vitamins are prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of final medium to 6.8 - 7.0. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium chloride, magnesium chloride, magnesium sulfate, ammonium chloride, calcium chloride, potassium phosphate, sodium chloride, trace elements, ferrous ammonium sulfate, sodium acetate, yeast extract, trypticase peptone, resazurin, sodium bicarbonate, isopropyl alcohol (propan-2-ol), vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 372
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium372.pdf 372. HALOBACTERIA MEDIUM Yeast extract 5.00 g Casamino acids 5.00 g Na-glutamate 1.00 g KCl 2.00 g Na3-citrate 3.00 g MgSO4 x 7 H2O 20.00 g NaCl 200.00 g FeCl2 x 4 H2O 36.00 mg MnCl2 x 4 H2O 0.36 mg Agar 20.00 g Add distilled water to give a final volume of 1000 ml. Adjust pH to 7.0 - 7.2 © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of yeast extract, casamino acids, monosodium glutamate, potassium chloride, sodium citrate, magnesium sulfate, sodium chloride, ferrous chloride, manganese chloride, and agar.
DSMZ Medium 370
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, yeast extract, and sodium ascorbate. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium370.pdf 370. HELIOBACTERIUM CHLORUM MEDIUM K2HPO4 1.0 g MgSO4 x 7 H2O 1.0 g Yeast extract 10.0 g Distilled water 1000.0 ml Adjust pH to 7.0. Boil the medium for few minutes under nitrogen gas. Add sodium ascorbate (0.5 g) and continue to bubble with nitrogen gas. Adjust the pH to 6.8. Fill immediately about 45 ml medium into 50 ml screw-capped bottles with rubber septum and screw tighten. Autoclave at 121˚C for 15 min. Incubate in low light. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 371
An organic-rich, solid culture medium comprised of potassium phosphate, potassium chloride, ammonium chloride, magnesium sulfate, calcium sulfate, trace elements, agar, sodium chloride, disodium glutamate, yeast extract, casamino acids, and sodium carbonate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium371.pdf 371. NATRONOBACTERIA MEDIUM KH2PO4 1.00 g KCl 1.00 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.24 g CaSO4 x 2 H2O 0.17 g Trace element solution SL-10 (see medium 320) 1.00 ml Agar, if necessary 20.00 g NaCl 200.00 g Na2-glutamate 1.00 g Yeast extract 5.00 g Casamino acids 5.00 g Na2CO3 5.00 g Add distilled water to give a final volume of 1000 ml. Adjust pH to 6.5 before autoclaving! Sterilize Na2CO3 separate from medium, add after cooling. Check final pH to be 9.0 - 9.5. If agar medium is prepared, heat and dissolve agar before adding sodium chloride. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 390
A minerals-salts, liquid culture medium comprised of ammonium sulfate, potassium phosphate, magnesium sulfate, calcium chloride, ferric trichloride, manganese chloride, sodium tetraborate, zinc sulfate, copper chloride, sodium molybdate, vanadyl sulfate, cobalt sulfate, resazurin, and sodium sulfide. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium390.pdf 390. PYROBACULUM MEDIUM (NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g MnCl2 x 4 H2O 1.80 mg Na2B4O7 x 10 H2O 4.50 mg ZnSO4 x 7 H2O 0.22 mg CuCl2 x 2 H2O 0.05 mg Na2MoO4 x 2 H2O 0.03 mg VOSO4 x 2 H2O 0.03 mg CoSO4 0.01 mg Resazurin 0.50 mg Na2S x 9 H2O 0.50 g Substrates (sse below) Distilled water 1000.00 ml Dissolve ingredients (except sulfide and substrates), adjust pH to 6.0 and sparge with 100% N2 gas for at least 30 min to remove dissolved oxygen. After autoclaving add substrates as listed below from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of substrates can be autoclaved with the exception of sodium thiosulfate which should be sterilized by filtration. Prior to inoculation add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of the completed medium to the appropriate value. Substrates and further instructions are given below: For DSM 4184: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.0. For DSM 4185: Trypticase peptone 0.50 g Yeast extract 0.20 g Sulfur 20.00 g Add peptone and yeast extract from sterile, anoxic stock solutions and then aseptically distribute medium under N2 gas atmosphere in sterile, anoxic culture vessels containing already the appropriate amount of sterile sulfur powder (sterilized by steaming for 3 h on each of 3 succesive days). Adjust pH of completed medium to 6.0. For DSM 13380: Yeast extract 1.00 g Na2S2O3 x 5 H2O 1.00 g Adjust pH of completed medium to 7.0. For DSM 13514: Trypticase peptone 0.50 g Yeast extract 0.20 g Na2S2O3 x 5 H2O 2.00 g Adjust pH of completed medium to 6.8. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 390.1
DSM 4184 is Pyrobaculum islandicum DSM 4184
Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and sodium thiosulfate added.
DSMZ Medium 390.2
Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and elemental sulfur are added.
DSMZ Medium 390.3
Similar to DSMZ Medium 390, except yeast extract and sodium thiosulfate are added. The pH is increased to 7.0.
DSMZ Medium 390.4
DSM 13514 is Pyrobaculum arsenaticum DSM 13514
Similar to DSMZ Medium 390, except trypticase peptone, yeast extract, and sodium thiosulfate are added. The pH is increased to 6.8.
DSMZ Medium 392
An organic-rich, solid culture medium comprised of glucose, peptone, yeast extract, potassium phosphate, magnesium sulfate, ferric trichloride, zinc sulfate, manganese sulfate, calcium chloride, thiamine hydrochloride, biotin, folic acid, inositol, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium392.pdf 392. BAF Medium Glucose 30.0 g Peptone 2.0 g Yeast extract 0.2 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.5 g FeCl3 x 6 H2O 10.0 mg ZnSO4 x 7 H2O 1.0 mg MnSO4 5.0 mg CaCl2 x 2 H2O 100.0 mg Thiamin HCl 50.0 μg Biotin 1.0 μg Folic acid 100.0 μg Inositol 50.0 μg Agar 15.0 g Distilled water 1000.0 ml pH 5.8 - 6.3. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 394
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium394.pdf 394. CHI 1776 MEDIUM Tryptone 25.0 g Yeast extract 7.5 g 1M Tris-HCl (pH 7.5) 20.0 ml Distilled water 1000.0 ml Autoclave, cool, and then add: 1M MgCl2 5.0 ml 1% Diaminopimelic acid 10.0 ml 0.4% Thymidine 10.0 ml 20% Glucose 25.0 ml Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, tris, magnesium chloride, diaminopimelic acid (2,6-diaminopimelic acid', thymidine, and glucose.
DSMZ Medium 393
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium393.pdf 393. YPD MEDIUM Yeast extract 10.0 g Peptone 20.0 g Glucose 20.0 g Distilled water 1000.0 ml Adjust pH to 6.5. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of yeast extract, peptone, and glucose.
DSMZ Medium 395
An organic-rich, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, yeast extract, starch, resazurin, elemental sulfur, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395.pdf 395. DESULFUROCOCCUS AMYLOLYTICUS MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Yeast extract 0.20 g Starch 5.00 g Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% N2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% N2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. For DSM 16532 omit sulfur. For DSM 18924 omit bicarbonate and supplement medium after autoclaving with 2.50 g/l D-glucose. Adjust pH of completed medium to 6.5. For DSM 19380 omit sulfur and increase amount of yeast extract to 0.50 g/l. Replace starch with 2.00 g/l Trypticase peptone as substrate. © 2013 DSMZ GmbH - All rights reserved
DSMZ Medium 395.1
Similar to DSMZ Medium 395, except elemental sulfur is omitted.
DSM 16532 is Desulfurococcus fermentans DSM 16532
DSMZ Medium 395.2
Similar to DSMZ Medium 395, except bicarbonate is omitted, and D-glucose is added. The pH is increased to 6.5.
DSM 18924 is Desulfurococcus kamchatkensis 1221n
DSMZ Medium 395.3
Similar to DSMZ Medium 395, except elemental sulfur and starch are omitted and trypticase peptone is added and the concentration of yeast extract is increased.
DSM 19380 is Fervidicoccus fontis Kam940
DSMZ Medium 395a
A minerals-salts, liquid culture medium comprised of ammonium chloride, potassium phosphate, potassium chloride, calcium chloride, magnesium chloride, sodium chloride, trace elements, resazurin, elemental sulfur, sodium bicarbonate, vitamins, and sodium sulfide. Prepared under an atmosphere of dinitrogen, dihydrogen, and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium395a.pdf 395a. BROCKIA LITHOTROPHICA MEDIUM NH4Cl 0.33 g KH2PO4 0.33 g KCl 0.33 g CaCl2 x 2 H2O 0.44 g MgCl2 x 6 H2O 0.70 g NaCl 0.50 g Trace elements SL-10 (see medium 320) 1.00 ml Resazurin 0.50 mg Sulfur, powdered 10.00 g NaHCO3 0.80 g Vitamin solution (see medium 141) 10.00 ml Na2S x 9 H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients (except sulfur, bicarbonate, vitamins and sulfide), boil medium for 1 min, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Adjust pH to 6.2 - 6.4, dispense under 80% H2 and 20% CO2 gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add sulfur aseptically to the medium while retaining anoxic conditions. Sulfur is sterilized separately by steaming for 3 h on each of 3 successive days. Bicarbonate is added from a sterile stock solution prepared under 80% N2 and 20% CO2 gas mixture. Vitamins (sterilized by filtration) and sulfide are added to the medium from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of completed medium to 6.5. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 396
An organic-rich, liquid culture medium comprised of yeast extract, trypticase peptone, sodium chloride, ammonium chloride, magnesium chloride, magnesium sulfate, potassium chloride, potassium phosphate, trace elements, resazurin, L-cysteine hydrochloride, sodium bicarbonate, sodium carbonate, trimethylamine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium396.pdf 396. METHANOSALSUM ZHILINAE MEDIUM Yeast extract (Difco) 2.00 g Trypticase (BBL) 2.00 g NaCl 40.00 g NH4Cl 1.00 g MgCl2 x 6 H2O 3.50 g MgSO4 x 7 H2O 3.00 g KCl 1.00 g K2HPO4 0.40 g Trace element solution (see medium 141) 5.00 ml Resazurin 0.50 mg L-Cysteine-HCl x H2O 0.50 g NaHCO3 3.00 g Na2CO3 2.00 g Trimethylamine-HCl 2.00 g Na2S x 9 H2O 0.25 g Distilled water 1000.00 ml Dissolve ingredients except cysteine, bicarbonate, carbonate, trimethylamine and sulfide, bring medium to the boil, then cool to room temperature under 100% N2 gas atmosphere. Add cysteine and dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. Add trimethylamine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas, whereas bicarbonate and carbonate are added from sterile anoxic stock solutions prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of completed medium to 9.2 - 9.4. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 398
An organic-rich liquid culture medium comprised of potassium phosphate, magnesium sulfate, calcium chloride, ammonium sulfate, yeast extract, glucose, and elemental sulfur.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium398.pdf 398. THERMOPLASMA VOLCANIUM MEDIUM KH2PO4 3.00 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.25 g (NH4)2 SO4 0.20 g Yeast extract 1.00 g Glucose 5.00 g Sulfur (for anaerobic media only) 4.00 g Distilled water 1000.00 ml Adjust pH of the mineral base to about 2 with sulfuric acid and autoclave. Add glucose and yeast extract from filter-sterilized stock solutions. For DSM 4301 add 0.5 g/l meat extract (Merck); aerobic growth of that strain is sometimes difficult to achieve. Check pH to be 2 to 3. For anaerobic growth, the acidified mineral base containing sulfur is filled into serum bottles (20 ml per 100 ml bottle) under N2 + CO2 (80 + 20) gas mixture. Then, the bottles are sterilized by tyndallisation. Yeast extract, glucose, and meat extract (for DSM 4301 only) are added from separately sterilized stock solutions. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 398.1
Similar to DSMZ Medium 398, except meat extract is added.
DSMZ Medium 398.2
Similar to DSMZ Medium 398, except prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 398.2 -< for DSM 4301, anaerobic growth
DSMZ Medium 397
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium397.pdf 397. FERULATE MEDIUM Use medium 212 without Na2SO4 and butyrate. Add sodium ferulate to 10 mM final concentration from filter-sterilized anaerobic stock solution. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 212, except sodium sulfate and butyrate are omitted. Sodium ferulate is added.
DSMZ Medium 413
An organic-rich, liquid culture medium comprised of lean ground beef hydrolysate, casitone, yeast extract, potassium phosphate, resazurin, cysteine, chopped meat, hemin solution, vitamin K solution, sodium formiate (sodium formate), and sodium fumarate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium413.pdf 413. BTU MEDIUM Medium 78 + Formiate/Fumarate: 78. Chopped Meat Medium Ground beef (fat free) 500.0 g Distilled water 1000.0 ml NaOH 1 N 25.0 ml Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add: Casitone 30.0 g Yeast extract 5.0 g K2HPO4 5.0 g Resazurin 1.0 mg Boil, cool under nitrogen atmosphere, add 0.5 g/l cysteine and adjust pH to 7.0. Dispense anoxically 7 ml medium into Hungate tubes containing meat particles (use 1 part meat particles to 4 or 5 parts fluid). Autoclave at 121˚C for 30 min. For agar slants use 15 g agar per 1000.0 ml medium. In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000 ml of medium after autoclaving: Haemin solution (see below) 10.0 ml Vitamin K1 or Vitamin K3 solution (see below) 0.2 ml Haemin solution: Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated. Vitamin K1 solution: Dissolve 0.1 ml of vitamin K1/K3 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Vitamin K3 solution: Dissolve 5 mg/ml of vitamin K3 in 10 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. Formiate/Fumarate Solution Na-formiate 6.0 g Na-fumarate 6.0 g Distilled water 100.0 ml Filter sterilize. Add 30 μl per ml of medium 78 before inoculation. © 2012 DSMZ GmbH - All rights reserved
DSMZ Medium 419
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium sulfate, magnesium sulfate, trace elements, sodium acetate, yeast extract, trypticase (peptone), sodium oxalate, resazurin, sodium carbonate, and L-cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium419.pdf 419. OXALOBACTER MEDIUM K2HPO4 0.25 g KH2PO4 0.25 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 25.00 mg Trace element sol. SL-10 (see medium 320) 1.00 ml Na-acetate 0.82 g Yeast extract 1.00 g Trypticase 1.00 g Na-oxalate 5.00 g Resazurin 0.50 mg Na2CO3 4.00 g L-Cysteine-HCl x H2O 0.50 g Distilled water 1000.00 ml Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere in anoxic Hungate-type tubes or seruim vials and autoclave. © 2015 DSMZ GmbH - All rights reserved
DSMZ Medium 414
An organic-rich, liquid culture medium comprised of trypticase peptone, casamino acids, yeast extract, glucose, monosodium glutamate, arginine, glycine, tryptopha, potassium phosphate, tween 80 (polysorbate 80), and cysteine hydrochloride.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium414.pdf 414. ACIDAMINOCOCCUS FERMENTANS MEDIUM Trypticase peptone 5.0 g Casamino acids 10.0 g Yeast extract 5.0 g Glucose 5.0 g Na-glutamate 4.0 g Arginine 1.0 g Glycine 1.0 g DL-Tryptophan 0.1 g KH2PO4 2.0 g Tween 80 0.5 ml Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml pH 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 421
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium421.pdf 421. CHROMATIUM SALEXIGENS MEDIUM Supplement medium 28 with NaCl (10%), MgCl2 x 6 H2O (0.3%) Na-acetate (0.05%) and sodium thiosulfate (0.05%). Incubate at 500 to 1000 Lux light intensity. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 28, except sodium chloride, magnesium chloride, sodium acetate, and sodium thiosulfate are added. The salinity is increased to hypersaline.
DSMZ Medium 420
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium420.pdf 420. BLOOD AGAR I Sterilize blood agar No 2 (Oxoid). After autoclaving cool to 50˚C, then add 40 ml/l of sterile horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). © 2007 DSMZ GmbH - All rights reserved
Similar to Oxoid blood agar No. 2, except horse blood is added.
DSMZ Medium 422
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium422.pdf 422. AMOEBOBACTER MEDIUM Supplement medium 28 with 2 mmol acetate and 1 mmol thiosulfate. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 28, except acetate and thiosulfate are added.
DSMZ Medium 423
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium423.pdf 423. XENORHABDUS MEDIUM Peptone 10.0 g Yeast extract 5.0 g NaCl 5.0 g Distilled water 1000.0 ml Dissolve and adjust pH to 7.2. Add 1.5% agar if required. Sterilize at 121˚C for 20 min. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of peptone, yeast extract, and sodium chloride.
DSMZ Medium 425
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium425.pdf 425. OATMEAL AGAR Oat flakes 10.0 g Oatmeal 10.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0 - 7.2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of oat flakes (rolled oats), oat meal, and agar.
DSMZ Medium 426
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium426.pdf 426. ORGANIC MEDIUM 79 Dextrose 10.0 g Peptone 10.0 g Casein peptone 2.0 g Yeast extract 2.0 g NaCl 6.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.8. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of dextrose (D-glucose), peptone, casein peptone (casitone), yeast extract, sodium chloride, and agar.
DSMZ Medium 428
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium428.pdf 428. HETEROTROPHIC MEDIUM H3P Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 850.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solution D: Yeast extract 1.000 g Na-acetate 1.000 g Na2-succinate 1.000 g DL-Malate 1.000 g Distilled water 30.000 ml pH 7.0 Solution E: Na-lactate 1.000 g Na-pyruvate 1.000 g D-Mannitol 1.000 g D-Glucose 2.000 g Distilled water 50.000 ml pH 7.0 filter-sterilized Standard vitamin solution: See next page Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 ml H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367). Solutions A, B, C and D are autoclaved separately for 15 min at 121˚C, cooled down to 50˚C and then mixed aseptically with filter-sterilized solution E (at 50˚C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, sodium phosphate, ammonium chloride, magnesium sulfate, calcium chloride, manganese chloride, sodium metavanadate, trace elements, ferric ammonium citrate, yeast extract, sodium acetate, sodium succinate, sodium malate, sodium lactate, sodium pyruvate, mannitol, glucose, and vitamins.
DSMZ Medium 427
An organic-rich, solid culture medium comprised of malt extract, glucose, asparagine, casein hydrolysate, potassium phosphate, magnesium sulfate, yeast extract, and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium427.pdf 427. MYKORRHIZA MEDIUM Malt extract 8.0 g Glucose 7.0 g Asparagine 0.5 g Casein hydrolysate 1.0 g KH2PO4 0.5 g MgSO4 x 7 H2O 0.5 g Yeast extract 1.0 g Agar 15.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 429
Similar to Columbia gar base, except horse blood is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429.pdf 429. COLUMBIA BLOOD AGAR Columbia agar base supplemented with 4% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). For rehydration of Helicobacter and Campylobacter spp., use medium 215 (brain heart infusion). 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. The same as Caso Bouillon (Merck or Oxoid, Germany). 215. BHI MEDIUM Brain Heart Infusion (Difco) 37.0 g Distilled water 1000.0 ml 1. NUTRIENT BROTH Peptone 5.0 g Meat extract 3.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 429b
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429b.pdf 429b. CHOCOLATE AGAR Mix autoclaved Columbia agar base solution with 10% horse or sheep blood, keep at 80˚C for 10 min. Mix well and pour plates after cooling to 50˚C. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. © 2007 DSMZ GmbH - All rights reserved
Similar to Columbia agar base, except horse blood or sheep blood is added.
DSMZ Medium 429a
Similar to Columbia agar base, except activated charcoal and horse blood are added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429a.pdf 429a. COLUMBIA BLOOD AGAR WITH CHARCOAL Add 2 g/l activated charcoal to the columbia agar base solution before autoclaving. Add 4% horse blood after cooling to 50˚C. Swirl agar during suspending. To prevent dry agar surface, spread about 1 ml of sterile medium or water over the agar plates and briefly allow to soak into the agar before inoculation. Incubate Neisseria strains in a humid microaerobic atmosphere. Rehydrate pellets of freeze-dried ampoules in liquid medium 220, 215 or 1. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 429d
Similar to Columbia agar base, except artificial seawater, horse blood or sheep blood, glucose, iron trichloride, and cysteine are addd. The concentration of agar is increased.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429d.pdf 429d. FRANCISELLA HALIOTICIDA MEDIUM Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50˚C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. © 2010 DSMZ GmbH - All rights reserved
DSMZ Medium 429c
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium429c.pdf 429c. COLUMBIA-GLUCOSE-CYSTEINE-AGAR Mix autoclaved Columbia agar base solution with 4% horse or sheep blood. After cooling to 50˚C add 1% glucose and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. © 2007 DSMZ GmbH - All rights reserved
Similar to Columbia agar base, except horse blood or sheep blood is added. Gluose and cysteine are also added.
DSMZ Medium 430
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, calcium chloride, magnesium chloride, sodium chlordie, sodium sulfate, sodium bicarbonate, sodium carbonate, vitamins, trace elements, sodium sulfide, and sodium acetate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium430.pdf 430. ECTOTHIORHODOSPIRA ABDELMALEKII MEDIUM KH2PO4 0.80 g NH4Cl 0.80 g CaCl2 x 2 H2O 0.05 g MgCl2 x 6 H2O 0.10 g NaCl 120.00 g Na2SO4 15.00 g NaHCO3 10.00 g Na2CO3 4 - 5.00g Vitamin solution VA * 1.00 ml Trace element solution SLA * 1.00 ml Na2S x 9 H2O 0.50 g Na-acetate 1.00 g Distilled water 1000.00 ml Adjust pH to 8.5, filter-sterilize and add appropriate amount of a sterile Na2S solution. *see medium 351 © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 432
Similar to DSMZ Medium 144, except the sodium chloride concentration is increased.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium432.pdf 432. HALOBACTEROIDES ACETOETHYLICUS MEDIUM Use medium no. 144 supplemented with 100 g/l of NaCl. Sterilize glucose, yeast extract and sodium sulfide separately (autoclave under nitrogen atmosphere). © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 431
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium chloride, calcium chloride, sodium bicarbonate, sodium chloride, trace elements, vitamins, sodium sulfide, sodium thiosulfate, and sodium malate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium431.pdf 431. ECTOTHIORHODOSPIRA VACUOLATA MEDIUM KH2PO4 1.00 g NH4Cl 0.50 g MgCl2 x 6 H2O 0.20 g CaCl2 x 2 H2O 0.10 g NaHCO3 3.00 g NaCl 30.00 g Trace element solution SLA* 1.00 ml Vitamin solution VA* 1.00 ml Na2S x 9 H2O 0.05 g Na2S2O3 x 5 H2O 0.50 g Na-malate 1.00 g Distilled water 1000.00 ml Adjust pH to 8.7, filter sterilize and add appropriate amount of Na2S solution. * see medium 351 © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 434
An organic-rich, liquid culture medium comprised of sodium chloride, magnesium chloride, magnesium sulfate, calcium chloride, potassium chloride, sodium bicarbonate, sodium bromide, proteose peptone, yeast extract, and glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium434.pdf 434. MARINOCOCCUS ALBUS - MEDIUM NaCl 81.000 g MgCl2 x 6 H2O 7.000 g MgSO4 x 7 H2O 9.600 g CaCl2 0.360 g KCl 2.000 g NaHCO3 0.060 g NaBr 0.026 g Proteose Peptone No. 3 5.000 g Yeast extract 10.000 g Glucose 1.000 g Distilled water 1000.000 ml Adjust pH to 7.2. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 435
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium435.pdf 435. KDM-2 MEDIUM Peptone 10.0 g Yeast extract 0.5 g Cysteine-HCl x H2O 1.0 g Agar 15.0 g Calf serum 200.0 ml Distilled water 800.0 ml Adjust pH to 6.5. Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. Add 2 g/l activated charcoal to the agar base solution before autoclaving. After autoclaving, cool to 45°C and add serum. © 2010 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of peptone, yeast extract, cysteine hydrochloride, agar, calf serum, and activated charcoal.
DSMZ Medium 438
Bordet Gengou Agar base supplemented with horse blood.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium438.pdf 438. BORDET-GENGOU-MEDIUM (DIFCO) Bordet-Gengou-Agar-Base supplemented with 15% horse blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 1. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 436
An organic-rich, liquid culture medium comprised of tryptone, yeast extract, glucose, cellobiose, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, resazurin, sodium carbonate, isobutyric acid, isovaleric acid, and 2-methylbutyric acid, and cysteine hydrochloride. Prepared under an atmosphere of carbon dioxide and dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium436.pdf 436. RUMINOCOCCUS ALBUS MEDIUM Tryptone 5.0 g Yeast extract 2.0 g Glucose 3.0 g Cellobiose 2.0 g Mineral solution 1 40.0 ml Mineral solution 2 40.0 ml Resazurin 1.0 mg Distilled water 920.0 ml Boil and cool under CO2. Under a gentle stream of CO2 add: Na2CO3 4.0 g Fatty acid mixture 1.0 ml Cysteine-HCl x H2O 500.0 mg Adjust pH to 7.0, distribute under N2 into appropriate vessels and sterilize. Mineral solution 1: K2HPO4 0.6 % Mineral solution 2: KH2PO4 0.6 % (NH4)2SO4 2.0 % NaCl 1.2 % MgSO4 x 7 H2O 0.25 % CaCl2 x 7 H2O 0.16 % Fatty acid mixture: Isobutyric acid 10.0 ml Isovaleric acid 10.0 ml 2-Methylbutyric acid 10.0 ml Distilled water 70.0 ml Inoculate under CO2 and incubate at 37˚C. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 439
Casman Agar base supplemented with sheep blood and lysed blood solution.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium439.pdf 439. CASMAN-MEDIUM Casman-Agar-Base (Difco) supplemented with 5% sheep blood and 0.15% water-lysed sheep blood. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 383.17
dsm 28890 is Desulfoplanes formicivorans
Similar to DSMZ Medium 383, except sodium formate and yeast extract are added.
amino acid solution
Defined organic solution comprised of a mixture of amino acids.
DSMZ Medium 412
The pH of this medium should be about 6.7 given the ratios of potassium phosphate.
An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, glucose, potassium phosphate, ammonium sulfate, sodium chloride, magnesium sulfate, calcium chloride, sodium acetate, vitamins, trace elements, resazurin, sodium bicarbonate, and cysteine hydrochloride. Prepared under an atmosphere of dinitrogen and carbon dioxide.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium412.pdf 412. ACETOMICROBIUM FAECALIS MEDIUM Trypticase peptone 2.000 g Yeast extract 2.000 g Glucose 4.000 g K2HPO4 0.225 g KH2PO4 0.255 g (NH4)2SO4 0.255 g NaCl 0.500 g MgSO4 x 7 H2O 0.100 g CaCl2 x 2 H2O 0.070 g Na-acetate 5.000 g Vitamin solution (see medium 141) 10.000 ml Trace element solution (see medium 141) 10.000 ml Resazurin 1.000 mg NaHCO3 6.000 g Cysteine-HCl x H2O 0.500 g Distilled water 1000.000 ml Autoclave under 2 bar N2:CO2 (80:20). © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 410
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, sodium sulfate, sodium chloride, calcium chloride, magnesium sulfate, sodium lactate, yeast extract, resazurin, ferrous sulfate, sodium thioglycolate, and ascorbic acid. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium410.pdf 410. DESULFOVIBRIO MEDIUM (BRACKISH WATER) Solution A: K2HPO4 0.5 g NH4Cl 1.0 g Na2SO4 1.0 g NaCl 10.0 g CaCl2 x 2 H2O 0.1 g MgSO4 x 7 H2O 2.0 g Na-DL-lactate 2.0 g Yeast extract 1.0 g Resazurin 1.0 mg Distilled water 980.0 ml Solution B: FeSO4 x 7 H2O 0.5 g Distilled water 10.0 ml Solution C: Na-thioglycolate 0.1 g Ascorbic acid 0.1 g Distilled water 10.0 ml Bring solution A to the boil, then cool to room temperature while gassing with 100% N2 gas. Add solutions B and C, adjust pH to 7.8 with NaOH, and distribute under N2 gas in anoxic Hungate-type tubes. During distribution continuously swirl the medium to keep the grey precipitate suspended. Autoclave 15 min at 121˚C. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 408
An organic-rich, liquid culture medium comprised of peptone, yeast extract, casamino acids, L-glutamic acid, ammonium chloride, potassium phosphate, sodium chloride, trace elements, resazurin, and L-cysteine hydrochloride. Prepared under an atmosphere of dinitrogen.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium408.pdf 408. PY-GLUTAMATE MEDIUM Peptone (Difco) 10.0 g Yeast extract 3.0 g Casamino acids 10.0 g L-Glutamic acid 20.0 g NH4Cl 2.0 g K2HPO4 x 3 H2O 2.0 g NaCl 25.0 g Trace elements solution (sse medium 141) 10.0 ml Resazurin 0.5 mg L-Cysteine-HCl x H2O 0.5 g Distilled water 1000.0 ml Dissolve ingredients (except cysteine), bring medium to the boil, then cool to room temperature under 100% N2 gas. Add and dissolve cysteine, adjust pH to 7.0 and dispense medium under 100% N2 gas atmosphere in culture vessels and autoclave. © 2014 DSMZ GmbH - All rights reserved
DSMZ Medium 407
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium407.pdf 407. SYNTROPHOMONAS SD2 MEDIUM Solution A: Mineral solution (see medium 212) 50.00 ml Trace element solution SL-10 (see medium 320) 1.00 ml Rumen fluid, clarified (see medium 1310) 50.00 ml Trypticase (BBL) 1.00 g Na2SO4 2.80 g Resazurin 1.00 mg Distilled water 790.00 ml Solution B: NaHCO3 3.50 g Distilled water 70.00 ml Solution C: Vitamin solution (see medium 503) 1.00 ml Solution D: Na-stearate 0.61 g Distilled water 20.00 ml Dissolve stearate by heating in water bath. Solution E: L-Cysteine-HCl x H2O 0.30 g Distilled water 10.00 ml Solution F: Na2S x 9 H2O 0.30 g Distilled water 10.00 ml Add and dissolve ingredients of solution A, adjust pH to 7.2, bring to the boil, cool to room temperature under a stream of 80% N2 and 20% CO2 gas mixture, then distribute under the same gas atmosphere in culture vials and autoclave. Solution B is filter-sterilized and then equilibrated with the 80% N2 and 20% CO2 gas mixture for at least 15 min. Solution C is prepared under 100% N2 gas and sterilized by filtration. Solutions D, E and F are autoclaved under 100% N2 gas. To complete the medium, appropriate amounts of solutions B, C, D, E, and F are added to solution A. Adjust pH of final medium to 7.2. Note: Use 25 - 50% inoculum. Growth can be followed by the clearing of stearate. Maintain cultures at 25˚C. © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solution, trace elements, rumen fluid, trypticase (peptone), sodium sulfate, resazurin, sodium bicarbonate, vitamins, sodium stearate, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 405
An organic-rich, solid culture medium comprised of casitone, yeast extract, sodium acette, agar, and natural seawater or artificial seawater.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium405.pdf 405. SP2 MEDIUM Casitone (Difco) 1.00 g Yeast extract (Difco) 0.20 g Na-acetate 0.02 g Agar (Difco), if necessary 15.00 g Sea water-natural or artificial 1000.00 ml Adjust pH to 7.2. If natural sea water is used, the pH need not be adjusted. Artificial sea water: NaCl 24.70 g KCl 0.70 g MgSO4 x 7 H2O 6.30 g MgCl2 x 6 H2O 4.60 g CaCl2 x 2 H2O 1.30 g NaHCO3 0.20 g Distilled water 1000.00 ml To prevent precipitation, CaCl2 and NaHCO3 are autoclaved each as separate stock solutions in 10 ml distilled water. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 404
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium404.pdf 404. METHANOSPHAERA CUNICULI MEDIUM Mineral solution 1 (see below) 40.00 ml Mineral solution 2 (see below) 40.00 ml Fe(NH4)2(SO4)2 x 7 H2O 2.00 mg NiCl2 x 6 H2O 0.20 mg Trace elements (see medium 141) 10.00 ml Na-acetate x 3 H2O 3.00 g Yeast extract 1.00 g Trypticase 1.00 g Resazurin 0.50 mg NaHCO3 3.00 g Methanol 5.00 ml Vitamin solution (see medium 141) 10.00 ml L-Cysteine-HCl x H2O 0.30 g Na2S x 9 H2O 0.30 g Distilled water 900.00 ml Dissolve ingredients (except bicarbonate, methanol, vitamins, cysteine and sulfide), bring medium to the boil, then cool to room temperature under 80% H2 and 20% CO2 gas atmosphere. Add and dissolve bicarbonate and adjust pH to 7.0, then distribute under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add methanol, vitamins, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of final medium to 7.0 – 7.2. After inoculation, pressurize culture bottles with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. Mineral solution 1: K2HPO4 6.00 g Distilled water 1000.00 ml Mineral solution 2: KH2PO4 6.00 g (NH4)2SO4 6.00 g NaCl 12.00 g MgSO4 x 7 H2O 2.60 g CaCl2 x 2 H2O 0.16 g Distilled water 1000.00 ml © 2014 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of mineral solutions, ferrous ammonium sulfate, nickel chloride, trace elements, sodium acetate, yeast extract, trypticase (peptone), resazurin, sodium bicarbonate, methanol, vitamins, L-cysteine hydrochloride, and sodium sulfide. Prepared under an atmosphere of dihydrogen, carbon dioxide, and dinitrogen.
DSMZ Medium 403
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium403.pdf 403. BSK-MEDIUM Solution A: CMRL 1066/10 x Glutamin (GIBCO 042-1545) 100.0 ml Proteose peptone No 2 (DIFCO 0121-01-3) 5.0 g Proteose tryptone (DIFCO 0123-01) 1.0 g Proteose yeastolate (TC) (DIFCO 5577-15) 1.0 g HEPES buffer (acid) (SIGMA H3375) 6.0 g Na-citrate 0.7 g Glucose (α-, D+) 3.0 g Na-pyruvate 0.8 g N-Acetylglucosamine 0.4 g MgCl2 x 6 H2O 0.3 g L-Glutamine 0.1 g Aqua bidest. filter-sterilized 900.0 ml Stir slowly at 4 - 10˚C for 3 hours, adjust pH to 7.6 with 5 M NaOH. Filter sterilize. Solution B: Dissolve 14.0 g gelatine in 200 ml bidest. water, autoclave 15 minutes at 115˚C. Solution C: Dissolve 50.05 g bovine serum albumine, fract. V (important: Sigma No A9647) in 143 ml bidest. water, or use BSA-solution Sigma A7409. Solution D: Rabbit serum, inactivated (GIBCO 037-06120M) 86.0 ml Combine the warm (37˚C) solutions A, B, C, D, and filter-sterilize the warm medium. Solution B can also be added after filtration. Alternatively, buy supplemented or un-supplemented BSK-H medium (Fa. Bio&SELL or Sigma) and supplement the medium according to manufacturer’s advice. Sterilize the mixed and warm (37˚C) medium by filtration. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of CMRL 1066 medium with glutamine, Proteose Peptone No. 2, Proteose Tryptone (Bacto Tryptone), Proteose Yeastolate (Bacto TC Yeastolate, or Yeastolate), HEPES, sodium citrate, D-glucose, sodium pyruvate, N-acetylglucosamine, magnesium chloride, L-glutamine, gelatin, bovine serum albumin, and rabbit serum.
DSMZ Medium 402
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium402.pdf 402. ALICYCLOBACILLUS MEDIUM Solution A: CaCl2 x 2 H2O 0.25 g MgSO4 x 7 H2O 0.50 g (NH4)2 SO4 0.20 g Yeast extract 2.00 g Glucose 5.00 g KH2PO4 3.00 g Distilled water (for liquid medium) 1000.00 ml Distilled water (for solid medium) 500.00 ml Adjust pH to 4.0 Solution B: Trace element sol. SL-6 (see medium 27) 1.00 ml Solution C: Agar 15.00 g Distilled water 500.00 ml Sterilize separately. For liquid medium combine solution A (with 1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of calcium chloride, magnesium sulfate, ammonium sulfate, yeast extract, glucose, potassium phosphate, trace elements, and agar.
Oxoid Blood Agar Base No. 2
http://www.oxoid.com/uk/blue/prod_detail/prod_detail.asp?pr=CM0271&org=153&c=uk&lang=EN BLOOD AGAR BASE NO.2 Code: CM0271 An improved Blood Agar Base possessing enhanced nutritional properties suitable for the cultivation of fastidious pathogens and other micro-organisms. Typical Formula* gm/litre Proteose peptone 15.0 Liver digest 2.5 Yeast extract 5.0 Sodium chloride 5.0 Agar 12.0 pH 7.4 ± 0.2 @ 25°C * Adjusted as required to meet performance standards Directions Suspend 40g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 7% sterile blood. Mix with gentle rotation and pour into sterile dishes or other containers. Description Oxoid Blood Agar Base No.2 was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of delicate organisms without interfering with their haemolytic reactions. In comparison with fresh digest agar, Blood Agar Base No.2 may be shown to have equal or superior growth promoting properties and chromogenic bacteria grown on the Oxoid medium show enhanced pigment formation. Comparison with many other blood agars has shown that with Oxoid Blood Agar Base No.2 growth of many bacteria - especially the fastidious streptococci and pneumococci - is considerably improved, as shown by luxuriant and early colonial development. Oxoid Blood Agar Base No.2 is specified by the American Food and Drug Administration for the preparation of sheep blood agar1. Phillips2, described an improved medium for sporulation of Clostridium perfringens based on Blood Agar Base No.2 to which are added lysed horse blood, bile, sodium bicarbonate and quinoline. The medium induced significant sporulation in all of 100 strains of Clostridium perfringens isolated from human faeces.
An organic-rich, solid culture medium comprised of proteose peptone, liver digest, yeast extract, sodium chloride, and agar.
lean ground beef hydrolysate
A beef extract prepared from lean ground beef (chopped lean ground beef) and sodium hydroxide that has been treated using heating and filtration.
rabbit serum
A serum medium ingredient, derived from the serum (coagulated blood) from a rabbit (Oryctolagus cuniculus).
Proteose Peptone No. 2
A Proteose Peptone made from only porcine (Sus scrofa) protein (muscle tissue).
From: https://m.bdbiosciences.com/ca/cell-culture/media-supplements/media-supplements/ao-animal-origin/porcine/proteose-peptone-no-2/p/212120 BD Bacto™ Proteose Peptone No. 2 is an animal-origin, enzymatic digest of protein. Similar to BD Bacto™ Proteose Peptone, this supplement provides relatively high proteose content and varying concentrations of nutrients, vitamins, minerals, peptides of varying sizes, buffering capabilities, and nitrogen content. However, BD Bacto™ Proteose Peptone No. 2 contains only porcine origin protein. Customers have successfully used this supplement to produce toxin-based vaccines, such as Bordetella pertusis for human health and other animal health vaccines.
mineral solution 1 for DSMZ Medium 404
An inorganic salts solution comprised of potassium phosphate.
mineral solution 2 for DSMZ Medium 404
oat meal
Wikipedia:Oatmeal Oatmeal is oat groats (i.e. grains) that have been ground, steel-cut, crushed, or rolled. Ground oats are also called 'white oats'. The term 'oatmeal' is also used in the U.S. and parts of Canada to mean oat porridge.
Rolled oats that have been ground or crushed or steel-cut.
standard vitamin solution for DSMZ Medium 428
Bordet Gengou Agar Base
From: Bordet Gengou Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Bordet Gengou Agar Base, with the addition of glycerol and sterile blood, is used in qualitative procedures for the detection and isolation of Bordetella pertussis from clinical specimens. Summary and Explanation Bordet Gengou Blood Agar is used in clinical laboratories as a method of diagnosing whooping cough. Bordetella pertussis, the etiologic agent of this disease, may be isolated from aspirated bronchial or nasopharyngeal secretions, perinasal swabs or, perhaps with greater difficulty due to the diversity of flora, from throat swabs.1 Bordet and Gengou introduced the medium in 1906 as a method of maintaining stock cultures.2 In 1934, Kendrick and Eldering replaced the 50% human or rabbit blood recommended in the original formulation with 15% sheep blood to make the medium more practical for laboratories to produce for routine clinical procedures.3 Principles of the Procedure Bordet Gengou Blood Agar contains potato infusion and glycerol to supply the nutrients necessary to support the growth of B. pertussis. Defibrinated animal blood supplies additional nutrients and enables the detection of hemolytic reactions, which aid in the identification of B. pertussis. Formula Difco™ Bordet Gengou Agar Base Approximate Formula* Per Liter Potato, Infusion from 125 g......................................... 4.5 g Sodium Chloride.......................................................... 5.5 g Agar.......................................................................... 20.0 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 30 g of the powder in 1 L of purified water containing 10 g of glycerol. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Aseptically add 15% sterile, defibrinated blood to the medium at 45-50°C. Mix well. 5. Test samples of the finished product for performance using stable, typical control cultures.
An organic-rich, solid culture medium comprised of potato infusion, sodium chloride, agar, glycerol, and defibrinated blood.
water-lysed sheep blood
Lysed blood solution where the blood is sheep's (Ovis aries) blood.
corn starch
lysed blood solution
Prepared by lysing blood by adding water.
Casman Agar Base
From: Casman Agar Base (Becton-Dickinson Difco and BBL Manual of Microbiological Culture Media, 2nd edition): Intended Use Casman Agar Base is used for the cultivation of fastidious pathogenic organisms, such as Haemophilus influenzae and Neisseria gonorrhoeae, from clinical specimens. Summary and Explanation Members of the genus Haemophilus are fastidious microorganisms that require the addition of X and/or V growth factors for in vitro cultivation.1 Neisseria are also fastidious microorganisms with complex growth requirements.2 In 1947, Casman described a blood-enriched medium prepared without an infusion of fresh meat for cultivation of Haemophilus and gonococci.1 The medium was developed to replace previous formulations that required time-consuming preparations of fresh and heated blood and fresh meat infusion to supply the nutrients necessary for the growth of these fastidious organisms.2,3 Casman found that nicotinamide interfered with the activity of an enzyme in blood that inactivates V factor (NAD). Using unheated human blood, he found that amount of nicotinamide required for good growth of H. influenzae was inhibitory to gonococci.2 Therefore, he reduced the nicotinamide to a level that allowed good growth of gonococci. Formula BBL™ Casman Agar Base Approximate Formula* Per Liter Pancreatic Digest of Casein........................................ 11.5 g Peptic Digest of Animal Tissue...................................... 5.0 g Yeast Extract................................................................ 3.5 g Beef Extract.................................................................. 3.0 g Nicotinamide................................................................ 0.05 g p-Aminobenzoic Acid................................................... 0.05 g Dextrose...................................................................... 0.5 g Cornstarch................................................................... 1.0 g Sodium Chloride.......................................................... 5.0 g Agar.......................................................................... 13.5 g *Adjusted and/or supplemented as required to meet performance criteria. Directions for Preparation from Dehydrated Product 1. Suspend 43 g of powder in 1 L of purified water. Mix thoroughly. 2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3. Autoclave at 121°C for 15 minutes. 4. Cool to 45-50°C and add 5% sterile blood and 0.15% blood solution, made by lysing 1 part of blood with 3 parts of water. Alternatively, add 5% partially lysed blood. 5. Test samples of the finished product for performance using stable, typical control cultures.
An organic-rich, solid culture medium comprised of pancreatic digest of casein (casitone), peptic digest of animal tissue, yeast extract (peptone), beef extract, nicotinamide, p-aminobenzoic acid, dextrose (D-glucose), cornstarch, sodium chloride, agar, and lysed blood solution.
DSMZ Medium 465a
Similar to DSMZ Medium 465, except 2-hydroxybiphenyl (biphenyl-2-ol) is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium465a.pdf 465a. MINERAL MEDIUM WITH 2-HYDROXYBIPHENYL Prepare medium 465. Dissolve 2-hydroxybiphenyl in ethanol (50 g/l) and filter sterilize. Pipette a volume of this stock solution into a sterile culture vessel so that the final concentration of 2-hydroxybiphenyl will be 0.5 g/l medium. Let the ethanol evaporate under sterile conditions. Add medium 465. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 464
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium464.pdf 464. PLATE COUNT AGAR Tryptone 5.0 g Yeast extract 2.5 g Dextrose 1.0 g Agar 15.0 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of tryptone, yeast extract, dextrose (D-glucose), and agar.
DSMZ Medium 463a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463a.pdf 463a. EDTA-MEDIUM Na2HPO4 x 2 H2O 0.41 g KH2PO4 0.26 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.40 g Trace element solution 1.00 ml Vitamin solution 1.00 ml Ethylenediamintetraacetic acid (EDTA), di- or trisodium salt 0.50 g Distilled water 1000.00 ml pH 8.0 Trace element solution: FeCl2 x 4 H2O 1.50 g ZnCl2 68.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 62.00 mg CoCl2 x 6 H2O 120.00 mg CuCl2 x 2 H2O 17.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 24.00 mg HCl, 0.05 molar 1000.00 ml Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. © 2010 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, calcium chloride, trace elements, vitamins, and EDTA.
DSMZ Medium 462a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462a.pdf 462a. PLATES WITH FLUORANTHENE Pour plates of medium 462. Dissolve 2 mg/ml fluoranthene in acetone and spread 1 ml of this solution on the surface of every pre-dried plate. Allow the acetone to evaporate under sterile conditions. Inoculate as usual. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 462, except fluoranthene is added.
DSMZ Medium 463
A minerals-salts, liquid medium comprised of sodium phosphate, potassium phosphate, magnesium sulfate, calcium chloride, trace elements, vitamins, and sodium nitrilotriacetate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium463.pdf 463. NITRILOTRIACETATE MEDIUM Na2HPO4 x 2 H2O 0.41 g KH2PO4 0.26 g MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.20 g Trace element solution 1.00 ml Vitamin solution 1.00 ml Nitrilotriacetate 1.00 g Distilled water 1000.00 ml pH 6.5 Trace element solution: FeCl2 x 4 H2O 1.50 g ZnCl2 68.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 62.00 mg CoCl2 x 6 H2O 120.00 mg CuCl2 x 2 H2O 17.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 24.00 mg HCl, 0.05 molar 1000.00 ml Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. © 2008 DSMZ GmbH - All rights reserved
trace elements solution for DSMZ Medium 463
A trace elements solution comprised of ferrous chloride, zinc chloride, manganese chloride, boric acid, cobalt chloride, copper chloride, nickel chloride, sodium molybdate, and hydrogen chloride.
DSMZ Medium 461c
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461c.pdf 461c. MINERAL MEDIUM WITH O-NITROPHENOL Dissolve 0.5 g o-nitrophenol per liter phosphate buffer (50 mM, pH 7.5) and sterilize by filtration. Prepare and autoclave medium 461 (vitamins not necessary). Add 200 ml nitrophenol solution to 800 ml medium 461. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 461, except o-nitrophenol (2-nitrophenol) is added.
DSMZ Medium 462
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, trace elements, and vitamins.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium462.pdf 462. MINERAL MEDIUM (BRUNNER) WITH VITAMINS Na2HPO4 2.44 g KH2PO4 1.52 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.05 g Trace element sol. SL-4 (see below) 10.00 ml Vitamin solution (Schlegel, see below) 2.50 ml Distilled water 1000.00 ml Adjust pH to 6.9. Rehydrate and cultivate lyophilized cells in the complex medium recommended for the specific strain (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 462 with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml Vitamin solution: p-Aminobenzoate 1.0 mg Biotin 0.2 mg Nicotinic acid 2.0 mg Thiamine-HCl x 2 H2O 1.0 mg Ca-pantothenate 0.5 mg Pyridoxamine 5.0 mg Vitamin B12 2.0 mg Distilled water 100.0 ml © 2010 DSMZ GmbH - All rights reserved
vitamin solution for DSMZ Medium 462
A vitamin solution comprised of p-aminobenzoate (4-aminobenzoate), biotin, nicotinic acid, thiamine hydrochloride, calcium pantothenate, pyridoxamine, and vitamin B12 (cobalamin).
DSMZ Medium 461b
Similar to DSMZ Meidum 461, except pyrrole-2-carboxylic acid and phosphate buffer are added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461b.pdf 461b. MINERAL MEDIUM WITH PYRROLIC ACID Dissolve 10 mol pyrrole-2-carboxylic acid per liter potassium phosphate buffer (50 mM; pH 7.2) and sterilize the solution by membrane filtration. Prepare and autoclave medium 461 (vitamins not necessary). Add 10 ml of pyrrolic acid solution per liter mineral medium. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 461
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, calcium chloride, magnesium sulfate, manganese sulfate, ammonium chloride, sodium chloride, vitamins, and trace elements.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461.pdf 461. MINERAL MEDIUM (NAGEL AND ANDREESEN) Distilled water 1000.00 ml Na2HPO4 x 2 H2O 1.45 g KH2PO4 0.25 g CaCl2 0.01 g MgSO4 x 7 H2O 0.50 g MnSO4 0.01 g NH4Cl 0.30 g NaCl 0.05 g Vitamin Solution 5.00 ml Trace element sol. SL-10 1.00 ml Dissolve and autoclave the phosphates separately. Add the vitamin solution and trace element solution after autoclaving. After mixing, pH should be 7.5. Rehydrate and cultivate the lyophilized cells in complex medium recommended for the specific strain (e.g. medium 1, 220 or 535) first. After this reactivation, cultivate on mineral medium 461 with the appropriate carbon source. Vitamin solution: Vitamin B12 50.00 mg Pantothenic acid 50.00 mg Riboflavin 50.00 mg Pyridoxamine-HCl 10.00 mg Biotin 20.00 mg Folic acid 20.00 mg Nicotinic acid 25.00 mg Nicotine amide 25.00 mg α-lipoic acid 50.00 mg p-aminobenzoic acid 50.00 mg Thiamine-HCl x 2 H2O 50.00 mg Distilled water 1000.00 ml Stir for some hours, filter sterilize the solution. Trace element solution SL-10: HCl (25%; 7.7 M) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml. © 2010 DSMZ GmbH - All rights reserved
vitamin solution for DSMZ Medium 461
A vitamin solution comprised of vitamin B12 (cobalamin), pantothenic acid, riboflavin, pyridoxamine hydrochloride, biotin, folic acid, nicotinic acid, nicotine amide (nicotinamide), alpha-lipoic acid (lipoic acid), p-aminobenzoic acid (4-aminobenzoic acid), and thiamine hydrochloride.
DSMZ Medium 461a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium461a.pdf 461a. MINERAL MEDIUM WITH QUINOLINE Prepare an emulsion of 3 g/l quinoline in 50 mM phosphate buffer by stirring or ultrasonication. Autoclave in a gas tight vessel. Add 100 ml of this emulsion to 1 l autoclaved medium 461. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 461, except quinoline is added.
DSMZ Medium 460
An organic-rich, liquid culture medium comprised of potassium phosphate, magnesium sulfate, sodium chloride, yeast extract, ferric chloride, and sodium malate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium460.pdf 460. NITROGEN-FREE MEDIUM FOR PSEUDOMONAS STUTZERI K2HPO4 0.5 g MgSO4 x 7 H2O 0.2 g NaCl 0.1 g Yeast extract 0.2 g FeCl3 x 6 H2O 15.0 mg DL-Na-malate 6.6 g Distilled water 1000.0 ml Adjust pH to 7.0. © 2007 DSMZ GmbH - All rights reserved
nitrogen-free medium for Pseudomonas stutzeri
DSMZ Medium 458
phenol degrading medium for Bacillus stearothermophilus
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium458.pdf 458. MEDIUM FOR BACILLUS STEAROTHERMOPHILUS - PHENOL DEGRADING K2HPO4 0.50 g NH4Cl 1.00 g MgSO4 x 7 H2O 0.02 g Yeast extract 0.20 g Casamino acids 0.10 g Distilled water 900.00 ml pH 7.4 Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l). © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of potassium phosphate, ammonium chloride, magnesium sulfate, yeast extract, casamino acids, trace elements, and phenol.
medium for Bacillus stearothermophilus - phenol degrading
DSMZ Medium 459
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium459.pdf 459. SUCROSE-PEPTONE-MEDIUM Peptone 20.0 g Sucrose 20.0 g Distilled water 1000.0 ml © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid culture medium comprised of peptone and sucrose.
DSMZ Medium 457d
Similar to DSMZ Medium 457, except biphenyl is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457d.pdf 457d. MEDIUM WITH BIPHENYL Prepare medium 457. Make a stock solution of 10 g/l biphenyl in ethanol and filter sterilize it using a cellulose filter membrane. Add an aliquot of this stock solution to a sterile flask so that the final concentration will be 0.25 g/l biphenyl, and let the ethanol evaporate. Add sterile medium to the crystal-layered flask. Alternatively, add biphenyl crystals directly to the liquid medium or to the lid of inversely incubated petri dishes containing 457-agar medium. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 457c
Similar to DSMZ Medium 457, except 3-chloroacrylic acid is added.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457c.pdf 457c. MEDIUM WITH CHLOROACRYLIC ACID Prepare medium 457. Make a stock solution of 4 g/l 3-chloroacrylic acid in water, neutralize and filter sterilize. Add 20 ml of this stock solution to 1 l of autoclaved medium. For better growth, 1 g/l glucose may be supplied additionally or instead of the chloroacrylic acid. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 457b.2
DSM 13022 is Pseudomonas frederiksbergensis
medium with fluoranthrene or phenanthrene
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457b.pdf 457b. MEDIUM WITH FLUORANTHENE OR PHENANTHRENE For DSM 7526, prepare medium 457 with 200 mg/l Tween 80. Prepare a solution of 2 g/l fluoranthene in acetone and filter sterilize using a cellulose filter membrane. Add 1 ml of this stock solution to the sterilized culture flask. Let acetone evaporate. Add 20 ml medium to the flask. Alternatively, the direct addition of about 1-2 mg fluoranthene to the medium is possible. For DSM 13022, prepare medium 457 with 0.3 g/l casamino acids. Add phenanthrene instead of fluoranthene to the culture vessel. 0.1 ml phenanthrene stock solution in acetone (5 mg/ml) are used per flask. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 457, except casamino acids and phenanthrene are added.
DSMZ Medium 457b.1
Similar to DSMZ Medium 457, except Tween 80 (polysorbate 80) and fluoranthene are added.
DSMZ Medium 457a
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457a.pdf 457a. MINERAL MEDIUM WITH 2-CHLOROBENZOATE Dissolve 78.3 g/l 2-chlorobenzoic acid in water by slow addition of concentrated NaOH (pH should reach 7.4). Filter-sterilize the solution and add 10.0 ml per liter autoclaved medium 457. Adjust the pH of the final medium to 7.4. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 457, except 2-chlorobenzoic acid (neutralized with sodium hydroxide) is added.
DSMZ Medium 456
An organic-rich, solid culture medium comprised of lactic acid whey, filtered white table wine, glucose, galactose, Tween 80 (polysorbate 80), and agar.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium456.pdf 456. KPL MEDIUM Lactic acid whey (see below) 1000.0 ml White table wine (see below) 140.0 ml Glucose 10.0 g Galactose 10.0 g Tween 80 1.0 ml Agar 15.0 g Adjust pH to 5.5 Lactic acid whey: Adjust 10% skim milk to pH 5.5 with lactic acid and heat at 100˚C for 30 minutes. Remove precipitate by filtration. For preparing broth medium, adjust filtrate to pH 7.0 with 2 N NaOH and heat at 100˚C for 30 minutes. Remove precipitate by filtration. Adjust filtrate to pH 5.5 with 2 N HCl. White table wine: Filter-sterilize and add to autoclaved basal medium. © 2007 DSMZ GmbH - All rights reserved
lactic acid whey
An undefined organic chemical mixture formed by heating diluted skim milk and lactic acid, then filtered. The pH is increased with sodium hydroxide, sample heated and re-filtered. The final pH is then adjusted by adding HCl (hydrogen chloride).
filtered white table wine
An undefined organic chemical mixture derived from the juice of the wine grape (the fruit of Vitis vinifera), formed through the process of maceration (homogenization) and fermentation, followed by filtration.
DSMZ Medium 457
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium457.pdf 457. MINERAL MEDIUM (BRUNNER) Na2HPO4 2.44 g KH2PO4 1.52 g (NH4)2SO4 0.50 g MgSO4 x 7 H2O 0.20 g CaCl2 x 2 H2O 0.05 g Trace element solution SL-4 (see below) 10.00 ml Distilled water 1000.00 ml Adjust pH to 6.9. Prepare a separate solution of the phosphates and autoclave separately. Combine the two solutions after cooling. Rehydrate and cultivate lyophilized cells in complex medium (e.g. medium 1, 220 or 535). After this reactivation, cultivate in mineral medium 457 with the appropriate carbon source. Trace element solution SL-4: EDTA 0.50 g FeSO4 x 7 H2O 0.20 g Trace element solution SL-6 (see below) 100.00 ml Distilled water 900.00 ml Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml © 2012 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of sodium phosphate, potassium phosphate, ammonium sulfate, magnesium sulfate, calcium chloride, and trace elements.
DSMZ Medium 454
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium454.pdf 454. EBA MEDIUM KH2PO4 0.20 g NaCl 1.00 g KCl 0.50 g MgSO4 x 7 H2O 0.50 g CaCl2 x 2 H2O 0.15 g NH4Cl 0.25 g Trace element sol. SL-10 (see medium 320) 1.00 ml Resazurin 1.00 mg Na2SeO3 x 5 H2O 1.00 ml (0.26 g/ 1000 ml 10 mM NaOH) Glycine 2.00 g Distilled water 950.00 ml pH 7.2 to 7.4 Before inoculation add per 100 ml of medium: Biotin (10 mg/l) 0.20 ml (autoclave for 10 minutes under N2) NaHCO3 (84 g/l) 3.00 ml (autoclaved under CO2) Na2S (60 g/l) 1.00 ml (autoclaved under N2) © 2007 DSMZ GmbH - All rights reserved
A minerals-salts, liquid culture medium comprised of potassium phosphate, sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, ammonium chloride, trace elements, resazurin, sodium selenite, glycine, biotin, sodium bicarbonate, and sodium sulfide. Prepared under an atmosphere of dinitrogen and carbon dioxide.
DSMZ Medium 455
An organic-rich, liquid culture medium comprised of trypticase peptone, yeast extract, soluble starch, meat infusion (meat extract), glucose, cysteine, calcium chloride, magnesium sulfate, ammonium sulfate, and potassium phosphate.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium455.pdf 455. AMB MEDIUM Trypticase peptone 4.00 g Yeast extract 5.00 g Starch, soluble 1.00 g Meat infusion 25.00 g Glucose 5.00 g Cysteine 1.00 g CaCl2 0.01 g MgSO4 0.20 g (NH4)2SO4 1.00 g K2HPO4 15.00 g Distilled water 1000.00 ml pH: 6.9. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 453
An organic-rich, liquid culture medium comprised of peptone from meat (meat peptone), peptone from casein (casitone), yeast extract, sodium chloride, and D-glucose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium453.pdf 453. STANDARD I MEDIUM Peptone from meat 7.8 g Peptone from caseine 7.8 g Yeast extract 2.8 g NaCl 5.6 g D(+)-Glucose 1.0 g Distilled water 1000.0 ml Autoclave for 15 min at 121˚C. pH of the final medium is 7.5. © 2012 DSMZ GmbH - All rights reserved
DSMZ Medium 452
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium452.pdf 452. GLUCOSE MEDIUM (NAKAYAMA) Glucose 10.00 g Peptone 10.00 g Yeast extract 15.00 g Agar 10.00 g Distilled water 980.00 ml Solution A 10.00 ml Solution B 10.00 ml Solution C 1.00 ml Solution A: KH2PO4 0.50 g K2HPO4 0.50 g Distilled water 100.00 ml Solution B: MgSO4 x 7 H2O 3.00 g NaCl 0.10 g MnSO4 x 5 H2O 0.10 g CuSO4 x 5 H2O 0.01 g Distilled water 100.00 ml Solution C: FeSO4 x 7 H2O 0.10 g Na3-Citrate 2.00 g Distilled water 100.00 ml Autoclave all solutions separately, final pH is 6.8. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, solid culture medium comprised of glucose, peptone, yeast extract, agar, potassium phosphate, magnesium sulfate, sodium chloride, manganese sulfate, coppersulfate, ferrous sulfate, and sodium citrate.
DSMZ Medium 449
An organic-rich, liquid culture medium comprised of peptone from casein (casitone), soya peptone (soy peptone), peptone bacteriological (peptone), yeast extract, ascorbic acid, magnesium sulfate, sodium glycerophosphate, and lactose.
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium449.pdf 449. M17 MEDIUM FOR LACTIC STREPTOCOCCI Peptone from casein 5.00 g Soya peptone 5.00 g Peptone bacteriological 5.00 g Yeast extract 2.50 g Ascorbic acid 0.50 g MgSO4 x 7 H2O 0.25 g Na2-ß-glycerophosphate 19.00 g Lactose 5.00 g Distilled water 1000.00 ml Adjust pH to 6.9 ± 0.2. Lactose should be sterilized by filtration. © 2007 DSMZ GmbH - All rights reserved
DSMZ Medium 450
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium450.pdf 450. SUPPLEMENTED (ARGININE) M9 MEDIUM To medium 382 add 20 mg/l arginine. © 2007 DSMZ GmbH - All rights reserved
Similar to DSMZ Medium 382, except arginine is added.
DSMZ Medium 441
http://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium441.pdf 441. DIAZOTROPHIC MEDIUM (RBA) Solution A: KH2PO4 0.100 g K2HPO4 0.900 g NaCl 0.100 g CaCl2 x 2 H2O 0.100 g MgSO4 x 7 H2O 0.100 g Na2MoO4 x 2 H2O 0.005 g NaVO3 x H2O 0.005 g MnSO4 x H2O 0.005 g FeSO4 x 7 H2O 0.010 g Yeast extract 0.050 g Trace element sol. SL-6 (see medium 27) 3.000 ml Distilled water 950.000 ml Agar (if necessary) 15.000 g Adjust pH to 7.3. Solution B: Na2-succinate 1.000 g DL-Malate 2.000 g Na-pyruvate 1.000 g D-Mannitol 2.000 g D-Glucose 2.000 g Distilled water 50.000 ml Adjust pH to 7.3. Sterilize solution A separately at 121˚C for 15 min., cool to 50˚C and then mix aseptically with filter-sterilized solution B and 5.0 ml of filter-sterilized standard vitamin solution (see medium 428). RBA is an ammonium-free medium which has successfully been used for the isolation, growth and purity check of a broad spectrum of nitrogen fixing bacteria (Ref. 3363). For microaerophilic nitrogen-fixing bacteria use semisolid medium with 0.3% end concentration of agar and incubate the liquid cultures under 10% (v/v) air and 90% (v/v) N2. © 2007 DSMZ GmbH - All rights reserved
An organic-rich, liquid medium comprised of potassium phosphate, sodium chloride, calcium chloride, magnesium sulfate, sodium molybdate, sodium metavanadate, manganese sulfate, ferrous sulfate, yeast extract, and trace elements. Prepared under an atmosphere of air and dinitrogen.
DSMZ
DSMZ Medium 784.1
DSM 24923 is Natranaerobaculum magadiense
Similar to DSMZ Medium 784, except ethanol is omitted and sodium thiosulfate is added.
differentiated cyanobacterial filament
A cyanobacterial filament that is differentiated.
prokaryotic colony
A colony comprised of a prokaryotic cells.
cell pigmentation
Prokaryotic cell quality, where the cell is pigmented (in that it has a color imparted by a chemical pigment compound).
cell granulation
A prokaryotic cell quality, where the cell has intracellular granules.
cell size quality
Prokaryotic cell quality, where the cell has a distinctive size (width).
thylakoid quality
A prokaryotic cell part quality, where the cell contains thylakoids membranes.
pico-sized
Cell size quality, where the width of the cell is between 0.2 and 2 microns (um).
nano-sized
Cell size quality, where the width of the cell is between 2 and 20 microns (um).
filterability
Cell size quality, where the size of a prokaryotic cell is small enough to pass through a filter with a defined pore size.
Berkefeld N filterable cell size
A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with an intermediate pore size N (for Normal). Pore sizes average 0.43 microns in diameter.
Berkefeld V filterable cell size
A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with a smaller pore size V (for Viel). Pore sizes average 0.38 microns in diameter. This is the most common filter size used to study the filterability of microorganisms.
Berkefeld filterable cell size
Cell size quality, where the cell has a filterable cell size that is small enough to pass through a Berkefeld filter candle with a defined pore size (e.g. V, N, or W), the largest of which is 0.45 microns.
Berkefeld W filterable cell size
A Berkefeld filterable cell size that is small enough to pass through a Berkefeld filter candle with a wider pore size W (for Wenig). Pore sizes average 0.45 microns in diameter.
A rapid commercial standardized kit containing 32 assays to distinguish streptococci and enterococci.
www.mediray.co.nz/.../om_biomerieux_test-kits_ot-32600_package_insert-32600.pdf "rapid ID 32 STREP is a standardized system for the identification of streptococci and enterococci, and those most common related organisms, in 4 hours, which uses 32 miniaturized enzymatic tests, as well as a specific database. The complete list of those organisms that it is possible to identify with this system is given in the Identification Table at the end of this package insert. Reading and interpretation are carried out automatically or manually."
2,4,6-trichlorophenol fermentation assay
Assays for the ability of a microorganism to ferment 2,4,6-trichlorophenol.
Assays for the ability of a microorganism to ferment 2,4-dichlorophenol.
2,5-didehydro-D-gluconic acid fermentation assay
Assays for the ability of a microorganism to ferment 2,5-didehydro-D-gluconate (2,5-didehydro-D-gluconic acid).
2,5-dihydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 2,5-dihydroxybenzoate (2,5-dihydroxybenzoic acid).
2,6-dihydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 2,6-dihydroxybenzoate (2,6-dihydroxybenzoic acid).
2-amino-2-deoxy-D-gluconic acid fermentation assay
2-amino-2-deoxy-D-gluconate fermentation
Assays for the ability of a microorganism to ferment 2-amino-2-deoxy-D-gluconate (2-amino-2-deoxy-D-gluconic acid).
2-amino-2-deoxy-D-gluconate acidification
2,4,6-trichlorophenol assimilation assay
Assays for the ability of a microorganism to assimilate 2,4,6-trichlorophenol as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate caffeic acid as a sole source of carbon and energy.
2,6-dihydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2,6-dihydroxybenzoate (2,6-dihydroxybenzoic acid) as a sole source of carbon and energy.
2,5-dihydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2,5-dihydroxybenzoate (2,5-dihydroxybenzoic acid) as a sole source of carbon and energy.
2,5-didehydro-D-gluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2,5-didehydro-D-gluconate (2,5-didehydro-D-gluconic acid) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate 2,4-dichlorophenol as a sole source of carbon and energy.
A rapid commercial standardized kit containing 32 assays to distinguish anaerobic bacteria.
Clinical Microbiology and Infection Volume 5, Issue 6, June 1999, Pages 319-326 Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. J Downes, A King, J Hardie, and I Phillips. https://doi.org/10.1111/j.1469-0691.1999.tb00150.x
cadaverine assimilation assay
Assays for the ability of a microorganism to assimilate cadaverine as a sole source of carbon and energy.
butyric acid assimilation assay
Assays for the ability of a microorganism to assimilate butyrate as a sole source of carbon and energy.
butanone assimilation assay
Assays for the ability of a microorganism to assimilate butanone as a sole source of carbon and energy.
4-hydroxybenzaldehyde assimilation assay
Assays for the ability of a microorganism to assimilate 4-hydroxybenzaldehyde as a sole source of carbon and energy.
4-hydroxy-L-proline assimilation assay
Assays for the ability of a microorganism to assimilate 4-hydroxy-L-proline as a sole source of carbon and energy.
4-coumaric acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-coumarate (4-coumaric acid) as a sole source of carbon and energy.
4-chlorophenol assimilation assay
Assays for the ability of a microorganism to assimilate 4-chlorophenol as a sole source of carbon and energy.
4-aminobenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-aminobenzoate (4-aminobenzoic acid) as a sole source of carbon and energy.
3-phenylpropionic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-phenylpropionate (3-phenylpropionic acid) as a sole source of carbon and energy.
3-phenyllactic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-phenyllactate (3-phenyllactic acid) as a sole source of carbon and energy.
azelaic acid assimilation assay
Assays for the ability of a microorganism to assimilate azelaate (azelaic acid) as a sole source of carbon and energy.
3-hydroxy-4-methoxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-hydroxy-4-methoxybenzoate (3-hydroxy-4-methoxybenzoic acid) as a sole source of carbon and energy.
3-hydroxy-4-methoxybenzoate assimilation
3-coumaric acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-coumarate (3-coumaric acid) as a sole source of carbon and energy.
3-aminobenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3-aminobenzoate (3-aminobenzoic acid) as a sole source of carbon and energy.
3,4-dimethoxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3,4-dimethoxybenzoic acid as a sole source of carbon and energy.
3-(3,4-dihydroxyphenyl)propanoic acid assimilation assay
3-(3,4-dihydroxyphenyl)propanoic acid assimilation
Assays for the ability of a microorganism to assimilate 3-(3,4-dihydroxyphenyl)propanoic acid or 3-(3,4-dihydroxyphenyl)propanoate as a sole source of carbon and energy.
3,4-dihydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3,4-dihydroxybenzoate (3,4-dihydroxybenzoic acid) as a sole source of carbon and energy.
3,4,5-trimethoxycinnamic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3,4,5-trimethoxycinnamate (3,4,5-trimethoxycinnamic acid) as a sole source of carbon and energy.
3,4,5-trimethoxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 3,4,5-trimethoxybenzoate (3,4,5-trimethoxybenzoic acid) as a sole source of carbon and energy.
2-phenylethylamine assimilation assay
Assays for the ability of a microorganism to assimilate 2-phenylethylamine as a sole source of carbon and energy.
2-phenylethanol assimilation assay
Assays for the ability of a microorganism to assimilate 2-phenylethanol as a sole source of carbon and energy.
2-methylbutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-methylbutyrate (2-methylbutyric acid) as a sole source of carbon and energy.
2-methoxyethanol assimilation assay
Assays for the ability of a microorganism to assimilate 2-methoxyethanol as a sole source of carbon and energy.
2-ethoxyethanol assimilation assay
Assays for the ability of a microorganism to assimilate 2-ethoxyethanol as a sole source of carbon and energy.
2-deoxy-D-glucose assimilation assay
Assays for the ability of a microorganism to assimilate 2-deoxy-D-glucose as a sole source of carbon and energy.
2-coumaric acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-coumarate (2-coumaric acid) as a sole source of carbon and energy.
2-aminopentanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 2-aminopentanoic acid as a sole source of carbon and energy.
isovanillin assimilation assay
Assays for the ability of a microorganism to assimilate isovanillin as a sole source of carbon and energy.
2-aminopentanoic acid fermentation assay
Assays for the ability of a microorganism to ferment 2-aminopentanoic acid.
2-coumaric acid fermentation assay
Assays for the ability of a microorganism to ferment 2-coumarate (2-coumaric acid).
2-deoxy-D-glucose fermentation assay
Assays for the ability of a microorganism to ferment 2-deoxy-D-glucose.
2-ethoxyethanol fermentation assay
Assays for the ability of a microorganism to ferment 2-ethoxyethanol.
2-hydroxybutyric acid fermentation assay
Assays for the ability of a microorganism to ferment 2-hydroxybutyrate (2-hydroxybutyric acid).
2-methoxyethanol fermentation assay
Assays for the ability of a microorganism to ferment 2-methoxyethanol.
2-methylbutyric acid fermentation assay
Assays for the ability of a microorganism to ferment 2-methylbutyrate (2-methylbutyric acid).
2-oxobutanoic acid fermentation assay
Assays for the ability of a microorganism to ferment 2-oxobutanoate (2-oxobutanoic acid).
2-oxoglutaric acid fermentation assay
Assays for the ability of a microorganism to ferment 2-oxoglutaric acid (2-oxoglutarate).
2-oxopentanoic acid fermentation assay
Assays for the ability of a microorganism to ferment 2-oxopentanoate (2-oxopentanoic acid).
2-phenylethanol fermentation assay
Assays for the ability of a microorganism to ferment 2-phenylethanol.
2-phenylethylamine fermentation assay
Assays for the ability of a microorganism to ferment 2-phenylethylamine.
2'-deoxyadenosine fermentation assay
Assays for the ability of a microorganism to ferment 2'-deoxyadenosine.
3,4,5-trimethoxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 3,4,5-trimethoxybenzoate (3,4,5-trimethoxybenzoic acid).
3,4,5-trimethoxycinnamic acid fermentation assay
Assays for the ability of a microorganism to ferment 3,4,5-trimethoxycinnamate (3,4,5-trimethoxycinnamic acid).
3,4-dihydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 3,4-dihydroxybenzoate (3,4-dihydroxybenzoic acid).
3-(3,4-dihydroxyphenyl)propanoic acid fermentation assay
3-(3,4-dihydroxyphenyl)propanoic acid acidification
3-(3,4-dihydroxyphenyl)propanoic acid fermentation
Assays for the ability of a microorganism to ferment 3-(3,4-dihydroxyphenyl)propanoic acid (or 3-(3,4-dihydroxyphenyl)prpanoate).
3,4-dimethoxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 3,4-dimethoxybenzoic acid.
3-aminobenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 3-aminobenzoate (3-aminobenzoic acid).
3-coumaric acid fermentation assay
Assays for the ability of a microorganism to ferment 3-coumarate (3-coumaric acid).
3-hydroxy-4-methoxybenzoic acid fermentation assay
3-hydroxy-4-methoxybenzoate acidification
Assays for the ability of a microorganism to ferment 3-hydroxy-4-methoxybenzoate (3-hydroxy-4-methoxybenzoic acid).
3-hydroxy-4-methoxybenzoate fermentation
3-hydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 3-hydroxybenzoate (3-hydroxybenzoic acid).
3-hydroxybutyric acid fermentation assay
Assays for the ability of a microorganism to ferment 3-hydroxybutyrate (3-hydroxybutyric acid).
3-O-methyl-D-glucose fermentation assay
Assays for the ability of a microorganism to ferment 3-O-methyl-D-glucose.
3-phenyllactic acid fermentation assay
Assays for the ability of a microorganism to ferment 3-phenyllactate (3-phenyllactic acid).
3-phenylpropionic acid fermentation assay
Assays for the ability of a microorganism to ferment 3-phenylpropionate (3-phenylpropionic acid).
4-aminobenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 4-aminobenzoate (4-aminobenzoic acid).
4-chlorophenol fermentation assay
Assays for the ability of a microorganism to ferment 4-chlorophenol.
4-coumaric acid fermentation assay
Assays for the ability of a microorganism to ferment 4-coumarate (4-coumaric acid).
4-hydroxy-L-proline fermentation assay
Assays for the ability of a microorganism to ferment 4-hydroxy-L-proline.
4-hydroxybenzaldehyde fermentation assay
Assays for the ability of a microorganism to ferment 4-hydroxybenzaldehyde.
4-hydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment 4-hydroxybenzoate (4-hydroxybenzoic acid).
L-arginine fermentation assay
Assays for the ability of a microorganism to ferment L-arginine.
4-hydroxybutyric acid fermentation assay
Assays for the ability of a microorganism to ferment 4-hydroxybutyrate (4-hydroxybutyric acid).
4-hydroxyphenylacetic acid fermentation assay
Assays for the ability of a microorganism to ferment 4-hydroxyphenylacetate (4-hydroxyphenylacetic acid).
4-methoxycinnamic acid fermentation assay
Assays for the ability of a microorganism to ferment 4-methoxycinnamic acid.
4-oxopentanoic acid fermentation assay
Assays for the ability of a microorganism to ferment 4-oxopentanoate (4-oxopentanoic acid).
5-aminopentanoic acid fermentation assay
Assays for the ability of a microorganism to ferment 5-aminopentanoate (5-aminopentanoic acid).
5-oxoproline fermentation assay
Assays for the ability of a microorganism to ferment 5-oxoprolinate (5-oxoproline).
6-O-alpha-D-glucopyranosyl-D-fructofuranose fermentation assay
6-O-alpha-D-glucopyranosyl-D-fructofuranose fermentation
6-O-alpha-D-glucopyranosyl-D-fructofuranose acidification
Assays for the ability of a microorganism to ferment 6-O-alpha-D-glucopyranosyl-D-fructofuranose.
6-O-acetyl-D-glucose fermentation assay
Assays for the ability of a microorganism to ferment 6-O-acetyl-D-glucose.
alpha-cyclodextrin fermentation assay
Assays for the ability of a microorganism to ferment alpha-cyclodextrin.
acetamide fermentation assay
Assays for the ability of a microorganism to ferment acetamide.
acetic acid fermentation assay
Assays for the ability of a microorganism to ferment acetate (acetic acid).
acetoacetic acid fermentation assay
Assays for the ability of a microorganism to ferment acetoacetate (acetoacetic acid).
acetoin fermentation assay
Assays for the ability of a microorganism to ferment acetoin.
acetone fermentation assay
Assays for the ability of a microorganism to ferment acetone.
aconitic acid fermentation assay
Assays for the ability of a microorganism to ferment aconitic acid (aconitate).
acrylic acid fermentation assay
Assays for the ability of a microorganism to ferment acrylate (acrylic acid).
adenine fermentation assay
Assays for the ability of a microorganism to ferment adenine.
adenosine fermentation assay
Assays for the ability of a microorganism to ferment adenosine.
adenosine 5'-monophosphate fermentation assay
adenosine 5'-monophosphate acidification
Assays for the ability of a microorganism to ferment adenosine 5'-monophosphate.
adipic acid fermentation assay
Assays for the ability of a microorganism to ferment adipic acid (adipate).
agar fermentation assay
Assays for the ability of a microorganism to ferment agar.
agarose fermentation assay
Assays for the ability of a microorganism to ferment agarose.
Ala-Gly fermentation assay
Assays for the ability of a microorganism to ferment Ala-Gly.
L-alanyl-L-glutamic acid fermentation assay
Assays for the ability of a microorganism to ferment L-alanyl-L-glutamic acid.
Ala-His fermentation assay
Assays for the ability of a microorganism to ferment Ala-His.
Ala-Thr fermentation assay
Assays for the ability of a microorganism to ferment Ala-Thr.
alanine fermentation assay
Assays for the ability of a microorganism to ferment alanine.
alginic acid fermentation assay
Assays for the ability of a microorganism to ferment alginate (alginic acid).
allantoin fermentation assay
Assays for the ability of a microorganism to ferment allantoin.
aminobenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment aminobenzoate (aminobenzoic acid).
ammonium betaine fermentation assay
Assays for the ability of a microorganism to ferment ammonium betaine.
amylopectin fermentation assay
Assays for the ability of a microorganism to ferment amylopectin.
amylose fermentation assay
Assays for the ability of a microorganism to ferment amylose.
anthranilic acid fermentation assay
Assays for the ability of a microorganism to ferment anthranilate.
arabinitol fermentation assay
Assays for the ability of a microorganism to ferment arabinitol.
arabinogalactan fermentation assay
Assays for the ability of a microorganism to ferment arabinogalactan.
arabinose fermentation assay
Assays for the ability of a microorganism to ferment arabinose.
arginine fermentation assay
Assays for the ability of a microorganism to ferment arginine.
ascorbic acid fermentation assay
Assays for the ability of a microorganism to ferment ascorbate (ascorbic acid).
asparagine fermentation assay
Assays for the ability of a microorganism to ferment asparagine.
aspartic acid fermentation assay
Assays for the ability of a microorganism to ferment aspartic acid (aspartate).
azelaic acid fermentation assay
Assays for the ability of a microorganism to ferment azelaate (azelaic acid).
Assays for the ability of a microorganism to assimilate methyl D-galactoside as a sole source of carbon and energy.
beta-alanine fermentation assay
Assays for the ability of a microorganism to ferment beta-alanine (beta-alaninate).
beta-cyclodextrin fermentation assay
Assays for the ability of a microorganism to ferment beta-cyclodextrin.
beta-D-glucuronamide fermentation assay
Assays for the ability of a microorganism to ferment beta-D-glucuronamide.
benzoic acid fermentation assay
Assays for the ability of a microorganism to ferment benzoate (benzoic acid).
bromosuccinic acid fermentation assay
Assays for the ability of a microorganism to ferment bromosuccinate (bromosuccinic acid).
butan-1-ol fermentation assay
Assays for the ability of a microorganism to ferment butan-1-ol.
butane-2,3-dione assimilation assay
Assays for the ability of a microorganism to assimilate butane-2,3-dione as a sole source of carbon and energy.
butane-1,3-diol assimilation assay
Assays for the ability of a microorganism to assimilate butane-1,3-diol as a sole source of carbon and energy.
butan-1-ol assimilation assay
Assays for the ability of a microorganism to assimilate butan-1-ol as a sole source of carbon and energy.
Assays for the ability of a microorganism to ferment methyl D-glucoside.
benzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate benzoate (benzoic acid) as a sole source of carbon and energy.
beta-alanine assimilation assay
Assays for the ability of a microorganism to assimilate beta-alanine (beta-alaninate) as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate methyl D-glucoside as a sole source of carbon and energy.
aspartic acid assimilation assay
Assays for the ability of a microorganism to assimilate aspartic acid (aspartate) as a sole source of carbon and energy.
asparagine assimilation assay
Assays for the ability of a microorganism to assimilate asparagine as a sole source of carbon and energy.
ascorbic acid assimilation assay
Assays for the ability of a microorganism to assimilate ascorbate as a sole source of carbon and energy.
arginine assimilation assay
Assays for the ability of a microorganism to assimilate arginine as a sole source of carbon and energy.
arabinose assimilation assay
Assays for the ability of a microorganism to assimilate arabinose as a sole source of carbon and energy.
arabinogalactan assimilation assay
Assays for the ability of a microorganism to assimilate arabinogalactan as a sole source of carbon and energy.
arabinitol assimilation assay
Assays for the ability of a microorganism to assimilate arabinitol as a sole source of carbon and energy.
anthranilic acid assimilation assay
Assays for the ability of a microorganism to assimilate anthranilate as a sole source of carbon and energy.
amylose assimilation assay
Assays for the ability of a microorganism to assimilate amylose as a sole source of carbon and energy.
amylopectin assimilation assay
Assays for the ability of a microorganism to assimilate amylopectin as a sole source of carbon and energy.
ammonium betaine assimilation assay
Assays for the ability of a microorganism to assimilate ammonium betaine as a sole source of carbon and energy.
aminobenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate aminobenzoate (aminobenzoic acid) as a sole source of carbon and energy.
allantoin assimilation assay
Assays for the ability of a microorganism to assimilate allantoin as a sole source of carbon and energy.
alginic acid assimilation assay
Assays for the ability of a microorganism to assimilate alginate (alginic acid) as a sole source of carbon and energy.
alanine assimilation assay
Assays for the ability of a microorganism to assimilate alanine as a sole source of carbon and energy.
agarose assimilation assay
Assays for the ability of a microorganism to assimilate agarose as a sole source of carbon and energy.
agar assimilation assay
Assays for the ability of a microorganism to assimilate agar as a sole source of carbon and energy.
phenylethylamine assimilation assay
Assays for the ability of a microorganism to assimilate phenylethylamine as a sole source of carbon and energy.
adenine assimilation assay
Assays for the ability of a microorganism to assimilate adenine as a sole source of carbon and energy.
acrylic acid assimilation assay
Assays for the ability of a microorganism to assimilate acrylate (acrylic acid) as a sole source of carbon and energy.
acetone assimilation assay
Assays for the ability of a microorganism to assimilate acetone as a sole source of carbon and energy.
acetoin assimilation assay
Assays for the ability of a microorganism to assimilate acetoin as a sole source of carbon and energy.
acetamide assimilation assay
Assays for the ability of a microorganism to assimilate acetamide as a sole source of carbon and energy.
6-O-acetyl-D-glucose assimilation assay
Assays for the ability of a microorganism to assimilate 6-O-acetyl-D-glucose as a sole source of carbon and energy.
xylooligosaccharide assimilation assay
Assays for the ability of a microorganism to assimilate xylooligosaccharide as a sole source of carbon and energy.
5-aminopentanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 5-aminopentanoate (5-aminopentanoic acid) as a sole source of carbon and energy.
4-oxopentanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-oxopentanoate (4-oxopentanoic acid) as a sole source of carbon and energy.
4-methoxycinnamic acid assimilation assay
Assays for the ability of a microorganism to assimilate 4-methoxycinnamic acid as a sole source of carbon and energy.
carboxymethylcellulose assimilation assay
Assays for the ability of a microorganism to assimilate carboxymethylcellulose as a sole source of carbon and energy.
catechol assimilation assay
Assays for the ability of a microorganism to assimilate catechol as a sole source of carbon and energy.
cellobiose assimilation assay
Assays for the ability of a microorganism to assimilate cellobiose as a sole source of carbon and energy.
chitin assimilation assay
Assays for the ability of a microorganism to assimilate chitin as a sole source of carbon and energy.
chitosan assimilation assay
Assays for the ability of a microorganism to assimilate chitosan as a sole source of carbon and energy.
cholesterol assimilation assay
Assays for the ability of a microorganism to assimilate cholesterol as a sole source of carbon and energy.
choline assimilation assay
Assays for the ability of a microorganism to assimilate choline as a sole source of carbon and energy.
cinnamic acid assimilation assay
Assays for the ability of a microorganism to assimilate cinnamate (cinnamic acid) as a sole source of carbon and energy.
cinnamyl alcohol assimilation assay
Assays for the ability of a microorganism to assimilate cinnamyl alcohol as a sole source of carbon and energy.
citrulline assimilation assay
Assays for the ability of a microorganism to assimilate citrulline as a sole source of carbon and energy.
coniferol assimilation assay
Assays for the ability of a microorganism to assimilate coniferol as a sole source of carbon and energy.
coumaric acid assimilation assay
Assays for the ability of a microorganism to assimilate coumarate (coumaric acid) as a sole source of carbon and energy.
creatine assimilation assay
Assays for the ability of a microorganism to assimilate creatine as a sole source of carbon and energy.
creatinine assimilation assay
Assays for the ability of a microorganism to assimilate creatinine as a sole source of carbon and energy.
crotonic acid assimilation assay
Assays for the ability of a microorganism to assimilate crotonate (crotonic acid) as a sole source of carbon and energy.
crystalline cellulose assimilation assay
Assays for the ability of a microorganism to assimilate crystalline cellulose as a sole source of carbon and energy.
(1->3)-beta-D-glucan assimilation assay
Assays for the ability of a microorganism to assimilate (1->3)-beta-D-glucan as a sole source of carbon and energy.
cyclodextrin assimilation assay
Assays for the ability of a microorganism to assimilate cyclodextrin as a sole source of carbon and energy.
cyclohexanecarboxylic acid assimilation assay
Assays for the ability of a microorganism to assimilate cyclohexanecarboxylate (cyclohexanecarboxylic acid) as a sole source of carbon and energy.
cyclohexanol assimilation assay
Assays for the ability of a microorganism to assimilate cyclohexanol as a sole source of carbon and energy.
cysteine assimilation assay
Assays for the ability of a microorganism to assimilate cysteine as a sole source of carbon and energy.
L-cysteine assimilation assay
Assays for the ability of a microorganism to assimilate L-cysteine as a sole source of carbon and energy.
cystine assimilation assay
Assays for the ability of a microorganism to assimilate cystine as a sole source of carbon and energy.
L-cystine assimilation assay
Assays for the ability of a microorganism to assimilate L-cystine as a sole source of carbon and energy.
dodecanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate dodecanoate (dodecanoic acid) as a sole source of carbon and energy.
dimethylamine assimilation assay
Assays for the ability of a microorganism to assimilate dimethylamine as a sole source of carbon and energy.
dimethyl sulfoxide assimilation assay
Assays for the ability of a microorganism to assimilate dimethyl sulfoxide as a sole source of carbon and energy.
dimethyl sulfide assimilation assay
Assays for the ability of a microorganism to assimilate dimethyl sulfide as a sole source of carbon and energy.
dihydroxyacetone assimilation assay
Assays for the ability of a microorganism to assimilate dihydroxyacetone as a sole source of carbon and energy.
dextran assimilation assay
Assays for the ability of a microorganism to assimilate dextran as a sole source of carbon and energy.
deoxyribonucleic acid assimilation assay
Assays for the ability of a microorganism to assimilate deoxyribonucleic acid as a sole source of carbon and energy.
D-glucopyranose 6-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate D-glucopyranose 6-phosphate as a sole source of carbon and energy.
D-glucopyranose 6-phosphate assimilation
D-glucopyranose 1-phosphate assimilation assay
D-glucopyranose 1-phosphate assimilation
Assays for the ability of a microorganism to assimilate D-glucopyranose 1-phosphate as a sole source of carbon and energy.
cytosine assimilation assay
Assays for the ability of a microorganism to assimilate cytosine as a sole source of carbon and energy.
cyclohexanecarboxylic acid fermentation assay
Assays for the ability of a microorganism to ferment cyclohexanecarboxylate (cyclohexanecarboxylic acid).
cyclohexanol fermentation assay
Assays for the ability of a microorganism to ferment cyclohexanol.
cysteine fermentation assay
Assays for the ability of a microorganism to ferment cysteine.
L-cysteine fermentation assay
Assays for the ability of a microorganism to ferment L-cysteine.
cystine fermentation assay
Assays for the ability of a microorganism to ferment cystine.
L-cystine fermentation assay
Assays for the ability of a microorganism to ferment L-cystine.
alpha-D-glucose 1-phosphate fermentation assay
alpha-D-glucose 1-phosphate fermentation
Assays for the ability of a microorganism to ferment alpha-D-glucose 1-phosphate.
alpha-D-glucose 1-phosphate acidification
(1->3)-beta-D-glucan fermentation assay
Assays for the ability of a microorganism to ferment (1->3)-beta-D-glucan.
crystalline cellulose fermentation assay
Assays for the ability of a microorganism to ferment crystalline cellulose.
crotonic acid fermentation assay
Assays for the ability of a microorganism to ferment crotonate (crotonic acid).
creatine fermentation assay
Assays for the ability of a microorganism to ferment creatine.
coniferol fermentation assay
Assays for the ability of a microorganism to ferment coniferol.
citrulline fermentation assay
Assays for the ability of a microorganism to ferment citrulline.
cis-aconitic acid fermentation assay
Assays for the ability of a microorganism to ferment cis-aconitic acid (cis-aconitate).
cinnamyl alcohol fermentation assay
Assays for the ability of a microorganism to ferment cinnamyl alcohol.
cinnamic acid fermentation assay
Assays for the ability of a microorganism to ferment cinnamate (cinnamic acid).
choline fermentation assay
Assays for the ability of a microorganism to ferment choline.
cholesterol fermentation assay
Assays for the ability of a microorganism to ferment cholesterol.
chitosan fermentation assay
Assays for the ability of a microorganism to ferment chitosan.
chitin fermentation assay
Assays for the ability of a microorganism to ferment chitin.
L-alanine fermentation assay
Assays for the ability of a microorganism to ferment L-alanine.
L-aspartic acid fermentation assay
Assays for the ability of a microorganism to ferment L-aspartic acid (L-aspartate).
D-aspartic acid fermentation assay
Assays for the ability of a microorganism to ferment D-aspartic acid (D-aspartate).
D-alanine fermentation assay
Assays for the ability of a microorganism to ferment D-alanine.
D-glucopyranose 6-phosphate fermentation assay
D-glucopyranose 6-phosphate fermentation
Assays for the ability of a microorganism to ferment D-glucopyranose 6-phosphate.
D-glucopyranose 6-phosphate acidification
cellobiose fermentation assay
Assays for the ability of a microorganism to ferment cellobiose.
catechol fermentation assay
Assays for the ability of a microorganism to ferment catechol.
carnitine fermentation assay
Assays for the ability of a microorganism to ferment carnitine.
carboxymethylcellulose fermentation assay
Assays for the ability of a microorganism to ferment carboxymethylcellulose.
caffeic acid fermentation assay
Assays for the ability of a microorganism to ferment caffeic acid (cis-caffeate or trans-caffeate).
cadaverine fermentation assay
Assays for the ability of a microorganism to ferment cadaverine.
butyric acid fermentation assay
Assays for the ability of a microorganism to ferment butyrate (butyric acid).
butanone fermentation assay
Assays for the ability of a microorganism to ferment butanone.
butane-2,3-dione fermentation assay
Assays for the ability of a microorganism to ferment butane-2,3-dione.
butane-2,3-diol fermentation assay
Assays for the ability of a microorganism to ferment butane-2,3-diol.
butane-1,3-diol fermentation assay
Assays for the ability of a microorganism to ferment butane-1,3-diol.
gellan gum fermentation assay
Assays for the ability of a microorganism to ferment gellan gum.
gelatin fermentation assay
Assays for the ability of a microorganism to ferment gelatin.
gallic acid fermentation assay
Assays for the ability of a microorganism to ferment gallate (gallic acid).
alpha-D-galacturonic acid fermentation assay
Assays for the ability of a microorganism to ferment alpha-D-galacturonate (D-galacturonic acid).
galacturonic acid fermentation assay
Assays for the ability of a microorganism to ferment galacturonate (galacturonic acid).
L-lyxose fermentation assay
Assays for the ability of a microorganism to ferment L-lyxose.
galactose fermentation assay
Assays for the ability of a microorganism to ferment galactose.
L-galactono-1,4-lactone fermentation assay
Assays for the ability of a microorganism to ferment L-galactono-1,4-lactone.
galactonolactone fermentation assay
Assays for the ability of a microorganism to ferment galactonolactone.
galactaric acid fermentation assay
Assays for the ability of a microorganism to ferment galactaric acid (galactarate).
gamma-cyclodextrin fermentation assay
Assays for the ability of a microorganism to ferment gamma-cyclodextrin.
gamma-aminobutyric acid fermentation assay
Assays for the ability of a microorganism to ferment gamma-aminobutyrate (gamma-aminobutyric acid).
fusidic acid fermentation assay
Assays for the ability of a microorganism to ferment fusidic acid (fusidate).
fumaric acid fermentation assay
Assays for the ability of a microorganism to ferment fumaric acid (fumarate).
(R)-lactic acid fermentation assay
Assays for the ability of a microorganism to ferment (R)-lactate.
isovanillin fermentation assay
Assays for the ability of a microorganism to ferment isovanillin.
fucose fermentation assay
Assays for the ability of a microorganism to ferment fucose.
D-fructose 6-phosphate fermentation assay
Assays for the ability of a microorganism to ferment D-fructose 6-phosphate.
fructose 6-phosphate fermentation assay
Assays for the ability of a microorganism to ferment fructose 6-phosphate.
fructose fermentation assay
Assays for the ability of a microorganism to ferment fructose.
formic acid fermentation assay
Assays for the ability of a microorganism to ferment formate (formic acid).
ferulic acid fermentation assay
Assays for the ability of a microorganism to ferment ferulate (ferulic acid).
ethylene glycol fermentation assay
Assays for the ability of a microorganism to ferment ethylene glycol.
ethanolamine fermentation assay
Assays for the ability of a microorganism to ferment ethanolamine.
ethanol fermentation assay
Assays for the ability of a microorganism to ferment ethanol.
erythrose fermentation assay
Assays for the ability of a microorganism to ferment erythrose.
dodecanoic acid fermentation assay
Assays for the ability of a microorganism to ferment dodecanoate (dodecanoic acid).
An assay for the activity of beta-galactosidase in a microorganism. Uses the substrate resorufin-beta-D-galactopyranoside. Beta-galactosidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange.
dimethylamine fermentation assay
Assays for the ability of a microorganism to ferment dimethylamine.
dimethyl sulfoxide fermentation assay
Assays for the ability of a microorganism to ferment dimethyl sulfoxide.
dimethyl sulfide fermentation assay
Assays for the ability of a microorganism to ferment dimethyl sulfide.
dihydroxyacetone fermentation assay
Assays for the ability of a microorganism to ferment dihydroxyacetone.
dextrin fermentation assay
Assays for the ability of a microorganism to ferment dextrin.
dextran fermentation assay
Assays for the ability of a microorganism to ferment dextran.
deoxyribonucleic acid fermentation assay
Assays for the ability of a microorganism to ferment deoxyribonucleic acid.
decanoic acid fermentation assay
Assays for the ability of a microorganism to ferment decanoate (decanoic acid).
D-glucose 6-phosphate fermentation assay
Assays for the ability of a microorganism to ferment D-glucose 6-phosphate.
D-glucopyranose 1-phosphate fermentation assay
D-glucopyranose 1-phosphate fermentation
Assays for the ability of a microorganism to ferment D-glucopyranose 1-phosphate.
D-glucopyranose 1-phosphate acidification
cytosine fermentation assay
Assays for the ability of a microorganism to ferment cytosine.
erythrose assimilation assay
Assays for the ability of a microorganism to assimilate erythrose as a sole source of carbon and energy.
esculin assimilation assay
Assays for the ability of a microorganism to assimilate esculin as a sole source of carbon and energy.
ethanol assimilation assay
Assays for the ability of a microorganism to assimilate ethanol as a sole source of carbon and energy.
ethylene glycol assimilation assay
Assays for the ability of a microorganism to assimilate ethylene glycol as a sole source of carbon and energy.
ferulic acid assimilation assay
Assays for the ability of a microorganism to assimilate ferulate (ferulic acid) as a sole source of carbon and energy.
fructose assimilation assay
Assays for the ability of a microorganism to assimilate fructose as a sole source of carbon and energy.
fructose 6-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate fructose 6-phosphate as a sole source of carbon and energy.
fucose assimilation assay
Assays for the ability of a microorganism to assimilate fucose as a sole source of carbon and energy.
fusidic acid assimilation assay
Assays for the ability of a microorganism to assimilate fusidic acid (fusidate) as a sole source of carbon and energy.
gamma-cyclodextrin assimilation assay
Assays for the ability of a microorganism to assimilate gamma-cyclodextrin as a sole source of carbon and energy.
galactonolactone assimilation assay
Assays for the ability of a microorganism to assimilate galactonolactone as a sole source of carbon and energy.
galactose assimilation assay
Assays for the ability of a microorganism to assimilate galactose as a sole source of carbon and energy.
galacturonic acid assimilation assay
Assays for the ability of a microorganism to assimilate galacturonate (galacturonic acid) as a sole source of carbon and energy.
gallic acid assimilation assay
Assays for the ability of a microorganism to assimilate gallate (gallic acid) as a sole source of carbon and energy.
gellan gum assimilation assay
Assays for the ability of a microorganism to assimilate gellan gum as a sole source of carbon and energy.
Glu-Glu assimilation assay
Assays for the ability of a microorganism to assimilate Glu-Glu as a sole source of carbon and energy.
glucaric acid assimilation assay
Assays for the ability of a microorganism to assimilate glucaric acid (glucarate) as a sole source of carbon and energy.
glucitol assimilation assay
Assays for the ability of a microorganism to assimilate glucitol as a sole source of carbon and energy.
gluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate gluconate (gluconic acid) as a sole source of carbon and energy.
glucosamine assimilation assay
Assays for the ability of a microorganism to assimilate glucosamine as a sole source of carbon and energy.
glucose assimilation assay
Assays for the ability of a microorganism to assimilate glucose as a sole source of carbon and energy.
rac-methyl lactate assimilation assay
Assays for the ability of a microorganism to assimilate rac-methyl lactate as a sole source of carbon and energy.
N-acetylglucosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetylglucosamine as a sole source of carbon and energy.
methylamine assimilation assay
Assays for the ability of a microorganism to assimilate methylamine as a sole source of carbon and energy.
alpha-D-glucose 6-phosphate fermentation assay
alpha-D-glucose 6-phosphate fermentation
Assays for the ability of a microorganism to ferment alpha-D-glucose 6-phosphate.
alpha-D-glucose 6-phosphate acidification
glucoside fermentation assay
Assays for the ability of a microorganism to ferment glucoside.
glucosiduronic acid fermentation assay
Assays for the ability of a microorganism to ferment glucosiduronic acid (alpha-D-glucosiduronate or beta-D-glucosiduronate).
glucuronic acid fermentation assay
Assays for the ability of a microorganism to ferment glucuronate (glucuronic acid).
D-glucuronic acid fermentation assay
Assays for the ability of a microorganism to ferment D-glucuronate (D-glucuronic acid).
glutamic acid fermentation assay
Assays for the ability of a microorganism to ferment glutamic acid (glutamate).
L-glutamic acid fermentation assay
Assays for the ability of a microorganism to ferment L-glutamic acid (L-glutamate).
gamma-poly(L-glutamic acid) fermentation assay
Assays for the ability of a microorganism to ferment gamma-poly(L-glutamic acid).
gamma-poly(L-glutamic acid) acidification
gamma-poly(L-glutamic acid) fermentation
glutamine fermentation assay
Assays for the ability of a microorganism to ferment glutamine.
L-glutamine fermentation assay
Assays for the ability of a microorganism to ferment L-glutamine.
glutaric acid fermentation assay
Assays for the ability of a microorganism to ferment glutarate (glutaric acid).
glutathione fermentation assay
Assays for the ability of a microorganism to ferment glutathione.
Gly-Asp fermentation assay
Assays for the ability of a microorganism to ferment Gly-Asp.
Gly-Glu fermentation assay
Assays for the ability of a microorganism to ferment Gly-Glu.
Gly-Pro fermentation assay
Assays for the ability of a microorganism to ferment Gly-Pro.
glyceric acid fermentation assay
Assays for the ability of a microorganism to ferment glycerate (glyceric acid).
glycerol 1-phosphate fermentation assay
Assays for the ability of a microorganism to ferment glycerol 1-phosphate.
glycine fermentation assay
Assays for the ability of a microorganism to ferment glycine.
glycine betaine fermentation assay
Assays for the ability of a microorganism to ferment glycine betaine.
glycolaldehyde fermentation assay
Assays for the ability of a microorganism to ferment glycolaldehyde.
glycolic acid fermentation assay
Assays for the ability of a microorganism to ferment glycolate (glycolic acid).
Gly-Gln fermentation assay
Assays for the ability of a microorganism to ferment Gly-Gln.
Gly-Met fermentation assay
Assays for the ability of a microorganism to ferment Gly-Met.
glycyl-glycyl-glycine fermentation assay
Assays for the ability of a microorganism to ferment glycyl-glycyl-glycine.
glycylglycine fermentation assay
Assays for the ability of a microorganism to ferment glycylglycine.
glyoxylic acid fermentation assay
Assays for the ability of a microorganism to ferment glyoxylate (glyoxylic acid).
guanidine fermentation assay
Assays for the ability of a microorganism to ferment guanidine.
guanine fermentation assay
Assays for the ability of a microorganism to ferment guanine.
hemicellulose fermentation assay
Assays for the ability of a microorganism to ferment hemicellulose.
hexadecanoic acid fermentation assay
Assays for the ability of a microorganism to ferment hexadecanoate (hexadecanoic acid).
trans-caffeic acid fermentation assay
Assays for the ability of a microorganism to ferment trans-caffeate.
glucose fermentation assay
Assays for the ability of a microorganism to ferment glucose.
glucosamine fermentation assay
Assays for the ability of a microorganism to ferment glucosamine.
D-gluconic acid fermentation assay
Assays for the ability of a microorganism to ferment D-gluconate (D-gluconic acid).
cis-caffeic acid fermentation assay
Assays for the ability of a microorganism to ferment cis-caffeate.
glucitol fermentation assay
Assays for the ability of a microorganism to ferment glucitol.
D-glucaric acid fermentation assay
Assays for the ability of a microorganism to ferment D-glucaric acid (D-glucarate).
glucaric acid fermentation assay
Assays for the ability of a microorganism to ferment glucaric acid (glucarate).
Glu-Glu fermentation assay
Assays for the ability of a microorganism to ferment Glu-Glu.
propane-1,3-diol assimilation assay
Assays for the ability of a microorganism to assimilate propane-1,3-diol as a sole source of carbon and energy.
propane-1,2-diol assimilation assay
Assays for the ability of a microorganism to assimilate propane-1,2-diol as a sole source of carbon and energy.
propan-2-ol assimilation assay
Assays for the ability of a microorganism to assimilate propan-2-ol as a sole source of carbon and energy.
propan-1-ol assimilation assay
Assays for the ability of a microorganism to assimilate propan-1-ol as a sole source of carbon and energy.
proline assimilation assay
Assays for the ability of a microorganism to assimilate proline as a sole source of carbon and energy.
polysorbate 60 assimilation assay
Assays for the ability of a microorganism to assimilate polysorbate 60 as a sole source of carbon and energy.
hemicellulose assimilation assay
Assays for the ability of a microorganism to assimilate hemicellulose as a sole source of carbon and energy.
guanine assimilation assay
Assays for the ability of a microorganism to assimilate guanine as a sole source of carbon and energy.
guanidine assimilation assay
Assays for the ability of a microorganism to assimilate guanidine as a sole source of carbon and energy.
glycylglycine assimilation assay
Assays for the ability of a microorganism to assimilate glycylglycine as a sole source of carbon and energy.
glycyl-glycyl-glycine assimilation assay
Assays for the ability of a microorganism to assimilate glycyl-glycyl-glycine as a sole source of carbon and energy.
glycolic acid assimilation assay
Assays for the ability of a microorganism to assimilate glycolate as a sole source of carbon and energy.
glycolaldehyde assimilation assay
Assays for the ability of a microorganism to assimilate glycolaldehyde as a sole source of carbon and energy.
glycine betaine assimilation assay
Assays for the ability of a microorganism to assimilate glycine betaine as a sole source of carbon and energy.
glycine assimilation assay
Assays for the ability of a microorganism to assimilate glycine as a sole source of carbon and energy.
glycerol 1-phosphate assimilation assay
Assays for the ability of a microorganism to assimilate glycerol 1-phosphate as a sole source of carbon and energy.
glyceric acid assimilation assay
Assays for the ability of a microorganism to assimilate glycerate (glyceric acid) as a sole source of carbon and energy.
glutathione assimilation assay
Assays for the ability of a microorganism to assimilate glutathione as a sole source of carbon and energy.
glutaric acid assimilation assay
Assays for the ability of a microorganism to assimilate glutarate (glutaric acid) as a sole source of carbon and energy.
glutamine assimilation assay
Assays for the ability of a microorganism to assimilate glutamine as a sole source of carbon and energy.
gamma-poly(L-glutamic acid) assimilation assay
gamma-poly(L-glutamic acid) assimilation
Assays for the ability of a microorganism to assimilate gamma-poly(L-glutamic acid) as a sole source of carbon and energy.
glutamic acid assimilation assay
Assays for the ability of a microorganism to assimilate glutamic acid (glutamate) as a sole source of carbon and energy.
glucuronic acid assimilation assay
Assays for the ability of a microorganism to assimilate glucuronate (glucuronic acid) as a sole source of carbon and energy.
glucosiduronic acid assimilation assay
Assays for the ability of a microorganism to assimilate glucosiduronic acid (alpha-D-glucosiduronate or beta-D-glucosiduronate) as a sole source of carbon and energy.
glucoside assimilation assay
Assays for the ability of a microorganism to assimilate glucoside as a sole source of carbon and energy.
heptanoic acid fermentation assay
Assays for the ability of a microorganism to ferment heptanoate (heptanoic acid).
hexanoic acid fermentation assay
Assays for the ability of a microorganism to ferment hexanoate (hexanoic acid).
hippurate fermentation assay
Assays for the ability of a microorganism to ferment hippurate.
histidine fermentation assay
Assays for the ability of a microorganism to ferment histidine.
L-histidine fermentation assay
Assays for the ability of a microorganism to ferment L-histidine.
hyaluronic acid fermentation assay
Assays for the ability of a microorganism to ferment hyaluronate (hyaluronic acid).
hydantoin fermentation assay
Assays for the ability of a microorganism to ferment hydantoin.
hydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment hydroxybenzoate (hydroxybenzoic acid).
hydroxybutyric acid fermentation assay
Assays for the ability of a microorganism to ferment hydroxybutyric acid (hydroxybutyrate).
hydroxyproline fermentation assay
Assays for the ability of a microorganism to ferment hydroxyproline.
hydroxyquinoline fermentation assay
Assays for the ability of a microorganism to ferment hydroxyquinoline.
hypoxanthine fermentation assay
Assays for the ability of a microorganism to ferment hypoxanthine.
indole fermentation assay
Assays for the ability of a microorganism to ferment indole.
indole-3-acetic acid fermentation assay
Assays for the ability of a microorganism to ferment indole-3-acetate (indole-3-acetic acid).
inosine fermentation assay
Assays for the ability of a microorganism to ferment inosine.
isoamylol fermentation assay
Assays for the ability of a microorganism to ferment isoamylol.
isobutanol fermentation assay
Assays for the ability of a microorganism to ferment isobutanol.
isobutyric acid fermentation assay
Assays for the ability of a microorganism to ferment isobutyrate (isobutyric acid).
isocaproic acid fermentation assay
Assays for the ability of a microorganism to ferment isocaproate (isocaproic acid).
isocitric acid fermentation assay
Assays for the ability of a microorganism to ferment isocitric acid (isocitrate).
isoferulic acid fermentation assay
Assays for the ability of a microorganism to ferment isoferulic acid.
isoleucine fermentation assay
Assays for the ability of a microorganism to ferment isoleucine.
L-isoleucine fermentation assay
Assays for the ability of a microorganism to ferment L-isoleucine.
isovaleric acid fermentation assay
Assays for the ability of a microorganism to ferment isovalerate (isovaleric acid).
itaconic acid fermentation assay
Assays for the ability of a microorganism to ferment itaconic acid (itaconate).
ketogluconic acid fermentation assay
Assays for the ability of a microorganism to ferment ketogluconate (ketogluconic acid).
alpha-aminobutyric acid fermentation assay
Assays for the ability of a microorganism to ferment alpha-aminobutyrate (alpha-aminobutyric acid).
l-alaninamide fermentation assay
Assays for the ability of a microorganism to ferment l-alaninamide.
lactamide fermentation assay
Assays for the ability of a microorganism to ferment lactamide.
lactic acid fermentation assay
Assays for the ability of a microorganism to ferment lactate (rac-lactic acid, or 2-hydroxypropanoic acid).
(S)-lactic acid fermentation assay
Assays for the ability of a microorganism to ferment (S)-lactate.
lactose fermentation assay
Assays for the ability of a microorganism to ferment lactose.
alpha-lactose fermentation assay
Assays for the ability of a microorganism to ferment alpha-lactose.
lactulose fermentation assay
Assays for the ability of a microorganism to ferment lactulose.
laminarin fermentation assay
Assays for the ability of a microorganism to ferment laminarin.
lecithin fermentation assay
Assays for the ability of a microorganism to ferment lecithin.
Leu-Gly fermentation assay
Assays for the ability of a microorganism to ferment Leu-Gly.
leucine fermentation assay
Assays for the ability of a microorganism to ferment leucine.
L-leucine fermentation assay
Assays for the ability of a microorganism to ferment L-leucine.
lichenin fermentation assay
Assays for the ability of a microorganism to ferment lichenin.
lignin fermentation assay
Assays for the ability of a microorganism to ferment lignin.
lysine fermentation assay
Assays for the ability of a microorganism to ferment lysine.
L-lysine fermentation assay
Assays for the ability of a microorganism to ferment L-lysine.
lyxose fermentation assay
Assays for the ability of a microorganism to ferment lyxose.
methoxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate methoxybenzoate (methoxybenzoic acid) as a sole source of carbon and energy.
methoxybenzene assimilation assay
Assays for the ability of a microorganism to assimilate methoxybenzene as a sole source of carbon and energy.
methionine assimilation assay
Assays for the ability of a microorganism to assimilate methionine as a sole source of carbon and energy.
methanol assimilation assay
Assays for the ability of a microorganism to assimilate methanol as a sole source of carbon and energy.
L-lysine assimilation assay
Assays for the ability of a microorganism to assimilate L-lysine as a sole source of carbon and energy.
lysine assimilation assay
Assays for the ability of a microorganism to assimilate lysine as a sole source of carbon and energy.
lignin assimilation assay
Assays for the ability of a microorganism to assimilate lignin as a sole source of carbon and energy.
lichenin assimilation assay
Assays for the ability of a microorganism to assimilate lichenin as a sole source of carbon and energy.
leucine assimilation assay
Assays for the ability of a microorganism to assimilate leucine as a sole source of carbon and energy.
Leu-Gly assimilation assay
Assays for the ability of a microorganism to assimilate Leu-Gly as a sole source of carbon and energy.
lecithin assimilation assay
Assays for the ability of a microorganism to assimilate lecithin as a sole source of carbon and energy.
laminarin assimilation assay
Assays for the ability of a microorganism to assimilate laminarin as a sole source of carbon and energy.
lactose assimilation assay
Assays for the ability of a microorganism to assimilate lactose as a sole source of carbon and energy.
ketogluconic acid assimilation assay
Assays for the ability of a microorganism to assimilate ketogluconate (ketogluconic acid) as a sole source of carbon and energy.
isovaleric acid assimilation assay
Assays for the ability of a microorganism to assimilate isovalerate (isovaleric acid) as a sole source of carbon and energy.
L-isoleucine assimilation assay
Assays for the ability of a microorganism to assimilate L-isoleucine as a sole source of carbon and energy.
isoleucine assimilation assay
Assays for the ability of a microorganism to assimilate isoleucine as a sole source of carbon and energy.
isoferulic acid assimilation assay
Assays for the ability of a microorganism to assimilate isoferulic acid as a sole source of carbon and energy.
isocitric acid assimilation assay
Assays for the ability of a microorganism to assimilate isocitric acid (isocitrate) as a sole source of carbon and energy.
isocaproic acid assimilation assay
Assays for the ability of a microorganism to assimilate isocaproate (isocaproic acid) as a sole source of carbon and energy.
isobutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate isobutyrate (isobutyric acid) as a sole source of carbon and energy.
isobutanol assimilation assay
Assays for the ability of a microorganism to assimilate isobutanol as a sole source of carbon and energy.
isoamylol assimilation assay
Assays for the ability of a microorganism to assimilate isoamylol as a sole source of carbon and energy.
indole-3-acetic acid assimilation assay
Assays for the ability of a microorganism to assimilate indole-3-acetate (indole-3-acetic acid) as a sole source of carbon and energy.
indole assimilation assay
Assays for the ability of a microorganism to assimilate indole as a sole source of carbon and energy.
hypoxanthine assimilation assay
Assays for the ability of a microorganism to assimilate hypoxanthine as a sole source of carbon and energy.
hydroxyquinoline assimilation assay
Assays for the ability of a microorganism to assimilate hydroxyquinoline as a sole source of carbon and energy.
hydroxybutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate hydroxybutyric acid (hydroxybutyrate) as a sole source of carbon and energy.
hydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate hydroxybenzoate (hydroxybenzoic acid) as a sole source of carbon and energy.
hydantoin assimilation assay
Assays for the ability of a microorganism to assimilate hydantoin as a sole source of carbon and energy.
hyaluronic acid assimilation assay
Assays for the ability of a microorganism to assimilate hyaluronate (hyaluronic acid) as a sole source of carbon and energy.
histidine assimilation assay
Assays for the ability of a microorganism to assimilate histidine as a sole source of carbon and energy.
hippurate assimilation assay
Assays for the ability of a microorganism to assimilate hippurate as a sole source of carbon and energy.
hexanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate hexanoate (hexanoic acid) as a sole source of carbon and energy.
hexadecanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate hexadecanoate (hexadecanoic acid) as a sole source of carbon and energy.
heptanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate heptanoate (heptanoic acid) as a sole source of carbon and energy.
malic acid fermentation assay
Assays for the ability of a microorganism to ferment malate (malic acid).
Assays for the ability of a microorganism to ferment methyl D-galactoside.
(S)-malic acid fermentation assay
Assays for the ability of a microorganism to ferment (S)-malate(2-) or (S)-malic acid.
maleic acid fermentation assay
Assays for the ability of a microorganism to ferment maleate (maleic acid).
maltitol fermentation assay
Assays for the ability of a microorganism to ferment maltitol.
maltodextrin fermentation assay
Assays for the ability of a microorganism to ferment maltodextrin.
maltose fermentation assay
Assays for the ability of a microorganism to ferment maltose.
malvalic acid fermentation assay
Assays for the ability of a microorganism to ferment malvalic acid.
mannan fermentation assay
Assays for the ability of a microorganism to ferment mannan.
mannitol fermentation assay
Assays for the ability of a microorganism to ferment mannitol.
mannose fermentation assay
Assays for the ability of a microorganism to ferment mannose.
mannoside fermentation assay
Assays for the ability of a microorganism to ferment mannoside.
heptadecanoic acid fermentation assay
Assays for the ability of a microorganism to ferment margarate (heptadecanoic acid).
melibiose fermentation assay
Assays for the ability of a microorganism to ferment melibiose.
mesaconic acid fermentation assay
Assays for the ability of a microorganism to ferment mesaconic acid (mesaconate).
meso-2,6-diaminopimelic acid fermentation assay
meso-2,6-diaminopimelic acid acidification
meso-2,6-diaminopimelic acid fermentation
Assays for the ability of a microorganism to ferment meso-2,6-diaminopimelic acid (meso-2,6-diaminopimelate).
meso-tartaric acid fermentation assay
Assays for the ability of a microorganism to ferment meso-tartaric acid (meso-tartrate).
methanethiol fermentation assay
Assays for the ability of a microorganism to ferment methanethiol.
methanol fermentation assay
Assays for the ability of a microorganism to ferment methanol.
methionine fermentation assay
Assays for the ability of a microorganism to ferment methionine.
L-methionine fermentation assay
Assays for the ability of a microorganism to ferment L-methionine.
methoxybenzene fermentation assay
Assays for the ability of a microorganism to ferment methoxybenzene.
methoxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment methoxybenzoate (methoxybenzoic acid).
rac-methyl lactate fermentation assay
Assays for the ability of a microorganism to ferment rac-methyl lactate.
methyl (R)-lactate fermentation assay
Assays for the ability of a microorganism to ferment methyl (R)-lactate.
methyl alpha-D-galactoside fermentation assay
Assays for the ability of a microorganism to ferment methyl alpha-D-galactoside.
methyl alpha-D-galactoside acidification
methyl beta-D-galactoside fermentation assay
Assays for the ability of a microorganism to ferment methyl beta-D-galactoside.
N-acetylglucosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetylglucosamine.
methyl pyruvate fermentation assay
Assays for the ability of a microorganism to ferment methyl pyruvate.
methylamine fermentation assay
Assays for the ability of a microorganism to ferment methylamine.
Assays for the ability of a microorganism to ferment methyl glucoside.
methylmalonic acid fermentation assay
Assays for the ability of a microorganism to ferment methylmalonic acid (methylmalonate).
monomethyl succinate fermentation assay
Assays for the ability of a microorganism to ferment monomethyl succinate.
myo-inositol fermentation assay
Assays for the ability of a microorganism to ferment myo-inositol.
N-acetyl-beta-D-galactosamine fermentation assay
N-acetyl-beta-D-galactosamine acidification
Assays for the ability of a microorganism to ferment N-acetyl-beta-D-galactosamine.
N-acetyl-beta-D-galactosamine fermentation
N-acetyl-beta-D-mannosamine fermentation assay
N-acetyl-beta-D-mannosamine acidification
Assays for the ability of a microorganism to ferment N-acetyl-beta-D-mannosamine.
N-acetyl-beta-D-mannosamine fermentation
N-acetyl-L-glutamic acid fermentation assay
Assays for the ability of a microorganism to ferment N-acetyl-L-glutamic acid.
N-acetylneuraminic acid fermentation assay
Assays for the ability of a microorganism to ferment N-acetylneuraminic acid (N-acetylneuraminate).
N-carbamoylglycine fermentation assay
Assays for the ability of a microorganism to ferment N-carbamoylglycine.
N-carbamoylsarcosine fermentation assay
Assays for the ability of a microorganism to ferment N-carbamoylsarcosine.
N-methyl-D-aspartic acid fermentation assay
Assays for the ability of a microorganism to ferment N-methyl-D-aspartic acid.
N-methyl-L-alanine fermentation assay
Assays for the ability of a microorganism to ferment N-methyl-L-alanine.
N-methylhydantoin fermentation assay
Assays for the ability of a microorganism to ferment N-methylhydantoin.
N-methylnicotinic acid fermentation assay
Assays for the ability of a microorganism to ferment N-methylnicotinate (N-methylnicotinic acid).
N,N-dimethylethanolamine fermentation assay
Assays for the ability of a microorganism to ferment N,N-dimethylethanolamine.
N,N-dimethylglycine fermentation assay
Assays for the ability of a microorganism to ferment N,N-dimethylglycine.
nonanoic acid fermentation assay
Assays for the ability of a microorganism to ferment nonanoate (nonanoic acid).
o-cresol fermentation assay
Assays for the ability of a microorganism to ferment o-cresol.
methanethiol assimilation assay
Assays for the ability of a microorganism to assimilate methanethiol as a sole source of carbon and energy.
meso-2,6-diaminopimelic acid assimilation assay
meso-2,6-diaminopimelic acid assimilation
Assays for the ability of a microorganism to assimilate meso-2,6-diaminopimelic acid (meso-2,6-diaminopimelate) as a sole source of carbon and energy.
mesaconic acid assimilation assay
Assays for the ability of a microorganism to assimilate mesaconic acid (mesaconate) as a sole source of carbon and energy.
melibiose assimilation assay
Assays for the ability of a microorganism to assimilate melibiose as a sole source of carbon and energy.
heptadecanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate margarate (heptadecanoic acid) as a sole source of carbon and energy.
mannoside assimilation assay
Assays for the ability of a microorganism to assimilate mannoside as a sole source of carbon and energy.
mannose assimilation assay
Assays for the ability of a microorganism to assimilate mannose as a sole source of carbon and energy.
mannitol assimilation assay
Assays for the ability of a microorganism to assimilate mannitol as a sole source of carbon and energy.
malvalic acid assimilation assay
Assays for the ability of a microorganism to assimilate malvalic acid as a sole source of carbon and energy.
maltose assimilation assay
Assays for the ability of a microorganism to assimilate maltose as a sole source of carbon and energy.
maltodextrin assimilation assay
Assays for the ability of a microorganism to assimilate maltodextrin as a sole source of carbon and energy.
maltitol assimilation assay
Assays for the ability of a microorganism to assimilate maltitol as a sole source of carbon and energy.
maleic acid assimilation assay
Assays for the ability of a microorganism to assimilate maleate (maleic acid) as a sole source of carbon and energy.
malic acid assimilation assay
Assays for the ability of a microorganism to assimilate malate (malic acid) as a sole source of carbon and energy.
D-lyxose assimilation assay
Assays for the ability of a microorganism to assimilate D-lyxose as a sole source of carbon and energy.
lyxose assimilation assay
Assays for the ability of a microorganism to assimilate lyxose as a sole source of carbon and energy.
polysorbate 20 assimilation assay
Assays for the ability of a microorganism to assimilate polysorbate 20 as a sole source of carbon and energy.
polysorbate assimilation assay
Assays for the ability of a microorganism to assimilate polysorbate as a sole source of carbon and energy.
poly(hydroxybutyrate) assimilation assay
Assays for the ability of a microorganism to assimilate poly(hydroxybutyrate) or poly-beta-hydroxybutyrate as a sole source of carbon and energy.
polygalacturonates assimilation assay
Assays for the ability of a microorganism to assimilate polygalacturonates as a sole source of carbon and energy.
Assays for the ability of a microorganism to assimilate methyl glucoside as a sole source of carbon and energy.
picolinic acid assimilation assay
Assays for the ability of a microorganism to assimilate picolinate (picolinic acid) as a sole source of carbon and energy.
phthalic acid assimilation assay
Assays for the ability of a microorganism to assimilate phthalic acid (phthalate) as a sole source of carbon and energy.
phosphocholine assimilation assay
Assays for the ability of a microorganism to assimilate phosphocholine as a sole source of carbon and energy.
phosphatidylcholine assimilation assay
Assays for the ability of a microorganism to assimilate phosphatidylcholine as a sole source of carbon and energy.
phloroglucinol assimilation assay
Assays for the ability of a microorganism to assimilate phloroglucinol as a sole source of carbon and energy.
phenylalanine assimilation assay
Assays for the ability of a microorganism to assimilate phenylalanine as a sole source of carbon and energy.
phenol assimilation assay
Assays for the ability of a microorganism to assimilate phenol as a sole source of carbon and energy.
perseitol assimilation assay
Assays for the ability of a microorganism to assimilate perseitol as a sole source of carbon and energy.
pentenoic acid assimilation assay
Assays for the ability of a microorganism to assimilate pentenoate (pentanoic acid) as a sole source of carbon and energy.
pentan-1-ol assimilation assay
Assays for the ability of a microorganism to assimilate pentan-1-ol as a sole source of carbon and energy.
pentadecanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate pentadecanoate (pentadecanoic acid) as a sole source of carbon and energy.
p-cresol assimilation assay
Assays for the ability of a microorganism to assimilate p-cresol as a sole source of carbon and energy.
oxopentanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate oxopentanoates (oxopentanoic acid) as a sole source of carbon and energy.
oxaloacetic acid assimilation assay
Assays for the ability of a microorganism to assimilate oxaloacetic acid (oxaloacetate) as a sole source of carbon and energy.
oxalic acid assimilation assay
Assays for the ability of a microorganism to assimilate oxalate (oxalic acid) as a sole source of carbon and energy.
ornithine assimilation assay
Assays for the ability of a microorganism to assimilate ornithine as a sole source of carbon and energy.
oleic acid assimilation assay
Assays for the ability of a microorganism to assimilate oleic acid (oleate) as a sole source of carbon and energy.
octanol assimilation assay
Assays for the ability of a microorganism to assimilate octanol as a sole source of carbon and energy.
octanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate octanoate (octanoic acid) as a sole source of carbon and energy.
o-cresol assimilation assay
Assays for the ability of a microorganism to assimilate o-cresol as a sole source of carbon and energy.
nonanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate nonanoate (nonanoic acid) as a sole source of carbon and energy.
N,N-dimethylglycine assimilation assay
Assays for the ability of a microorganism to assimilate N,N-dimethylglycine as a sole source of carbon and energy.
N,N-dimethylethanolamine assimilation assay
Assays for the ability of a microorganism to assimilate N,N-dimethylethanolamine as a sole source of carbon and energy.
N-methylnicotinic acid assimilation assay
Assays for the ability of a microorganism to assimilate N-methylnicotinate (N-methylnicotinic acid) as a sole source of carbon and energy.
N-methylhydantoin assimilation assay
Assays for the ability of a microorganism to assimilate N-methylhydantoin as a sole source of carbon and energy.
N-methyl-L-alanine assimilation assay
Assays for the ability of a microorganism to assimilate N-methyl-L-alanine as a sole source of carbon and energy.
N-methyl-D-aspartic acid assimilation assay
Assays for the ability of a microorganism to assimilate N-methyl-D-aspartic acid as a sole source of carbon and energy.
N-carbamoylsarcosine assimilation assay
Assays for the ability of a microorganism to assimilate N-carbamoylsarcosine as a sole source of carbon and energy.
N-carbamoylglycine assimilation assay
Assays for the ability of a microorganism to assimilate N-carbamoylglycine as a sole source of carbon and energy.
N-acetyl-beta-D-mannosamine assimilation assay
N-acetyl-beta-D-mannosamine assimilation
Assays for the ability of a microorganism to assimilate N-acetyl-beta-D-mannosamine as a sole source of carbon and energy.
methylmalonic acid assimilation assay
Assays for the ability of a microorganism to assimilate methylmalonic acid (methylmalonate) as a sole source of carbon and energy.
beta-lactose assimilation assay
Assays for the ability of a microorganism to assimilate beta-lactose as a sole source of carbon and energy.
octanoic acid fermentation assay
Assays for the ability of a microorganism to ferment octanoate (octanoic acid).
octanol fermentation assay
Assays for the ability of a microorganism to ferment octanol.
oleic acid fermentation assay
Assays for the ability of a microorganism to ferment oleic acid (oleate).
ornithine fermentation assay
Assays for the ability of a microorganism to ferment ornithine.
L-ornithine fermentation assay
Assays for the ability of a microorganism to ferment L-ornithine.
oxalic acid fermentation assay
Assays for the ability of a microorganism to ferment oxalate (oxalic acid).
oxaloacetic acid fermentation assay
Assays for the ability of a microorganism to ferment oxaloacetic acid (oxaloacetate).
oxopentanoic acid fermentation assay
Assays for the ability of a microorganism to ferment oxopentanoates.
p-cresol fermentation assay
Assays for the ability of a microorganism to ferment p-cresol.
pectin fermentation assay
Assays for the ability of a microorganism to ferment pectin.
pentadecanoic acid fermentation assay
Assays for the ability of a microorganism to ferment pentadecanoate (pentadecanoic acid).
pentan-1-ol fermentation assay
Assays for the ability of a microorganism to ferment pentan-1-ol.
pentenoic acid fermentation assay
Assays for the ability of a microorganism to ferment pentenoate (pentanoic acid).
perseitol fermentation assay
Assays for the ability of a microorganism to ferment perseitol.
phenol fermentation assay
Assays for the ability of a microorganism to ferment phenol.
trehalose fermentation assay
Assays for the ability of a microorganism to ferment trehalose.
salicyl alcohol fermentation assay
Assays for the ability of a microorganism to ferment salicyl alcohol.
rutin fermentation assay
Assays for the ability of a microorganism to ferment rutin.
ribose fermentation assay
Assays for the ability of a microorganism to ferment ribose.
ribitol fermentation assay
Assays for the ability of a microorganism to ferment ribitol.
rhamnose fermentation assay
Assays for the ability of a microorganism to ferment rhamnose.
quinic acid fermentation assay
Assays for the ability of a microorganism to ferment quinate (quinic acid).
L-pyrrolysine fermentation assay
Assays for the ability of a microorganism to ferment L-pyrrolysine.
pyrrolysine fermentation assay
Assays for the ability of a microorganism to ferment pyrrolysine.
pyrogallol fermentation assay
Assays for the ability of a microorganism to ferment pyrogallol.
pyrazinecarboxamide fermentation assay
Assays for the ability of a microorganism to ferment pyrazinecarboxamide.
putrescine fermentation assay
Assays for the ability of a microorganism to ferment putrescine.
purines fermentation assay
Assays for the ability of a microorganism to ferment purines.
D-psicose fermentation assay
Assays for the ability of a microorganism to ferment D-psicose.
psicose fermentation assay
Assays for the ability of a microorganism to ferment psicose.
propionic acid fermentation assay
Assays for the ability of a microorganism to ferment propionate (propionic acid).
propanediol fermentation assay
Assays for the ability of a microorganism to ferment propanediol.
propane-1,3-diol fermentation assay
Assays for the ability of a microorganism to ferment propane-1,3-diol.
propane-1,2-diol fermentation assay
Assays for the ability of a microorganism to ferment propane-1,2-diol.
propan-2-ol fermentation assay
Assays for the ability of a microorganism to ferment propan-2-ol.
propan-1-ol fermentation assay
Assays for the ability of a microorganism to ferment propan-1-ol.
L-proline fermentation assay
Assays for the ability of a microorganism to ferment L-proline.
proline fermentation assay
Assays for the ability of a microorganism to ferment proline.
polysorbate 80 fermentation assay
Assays for the ability of a microorganism to ferment polysorbate 80.
polysorbate 60 fermentation assay
Assays for the ability of a microorganism to ferment polysorbate 60.
polysorbate 40 fermentation assay
Assays for the ability of a microorganism to ferment polysorbate 40.
polysorbate 20 fermentation assay
Assays for the ability of a microorganism to ferment polysorbate 20.
polysorbate fermentation assay
Assays for the ability of a microorganism to ferment polysorbate.
poly(hydroxybutyrate) fermentation assay
Assays for the ability of a microorganism to ferment poly(hydroxybutyrate) or poly-beta-hydroxybutyrate.
polygalacturonates fermentation assay
Assays for the ability of a microorganism to ferment polygalacturonates.
poly(ethylene glycol) fermentation assay
Assays for the ability of a microorganism to ferment poly(ethylene glycol).
picolinic acid fermentation assay
Assays for the ability of a microorganism to ferment picolinate (picolinic acid).
phthalic acid fermentation assay
Assays for the ability of a microorganism to ferment phthalic acid (phthalate).
phosphocholine fermentation assay
Assays for the ability of a microorganism to ferment phosphocholine.
phosphatidylcholine fermentation assay
Assays for the ability of a microorganism to ferment phosphatidylcholine.
phloroglucinol fermentation assay
Assays for the ability of a microorganism to ferment phloroglucinol.
L-phenylalanine fermentation assay
Assays for the ability of a microorganism to ferment L-phenylalanine.
phenylalanine fermentation assay
Assays for the ability of a microorganism to ferment phenylalanine.
phenylacetic acid fermentation assay
Assays for the ability of a microorganism to ferment phenylacetate (phenylacetic acid).
propanediol assimilation assay
Assays for the ability of a microorganism to assimilate propanediol as a sole source of carbon and energy.
psicose assimilation assay
Assays for the ability of a microorganism to assimilate psicose as a sole source of carbon and energy.
pullulan assimilation assay
Assays for the ability of a microorganism to assimilate pullulan as a sole source of carbon and energy.
purines assimilation assay
Assays for the ability of a microorganism to assimilate purines as a sole source of carbon and energy.
pyrazinecarboxamide assimilation assay
Assays for the ability of a microorganism to assimilate pyrazinecarboxamide as a sole source of carbon and energy.
pyrogallol assimilation assay
Assays for the ability of a microorganism to assimilate pyrogallol as a sole source of carbon and energy.
pyrrolysine assimilation assay
Assays for the ability of a microorganism to assimilate pyrrolysine as a sole source of carbon and energy.
L-pyrrolysine assimilation assay
Assays for the ability of a microorganism to assimilate L-pyrrolysine as a sole source of carbon and energy.
rhamnose assimilation assay
Assays for the ability of a microorganism to assimilate rhamnose as a sole source of carbon and energy.
D-ribitol assimilation assay
Assays for the ability of a microorganism to assimilate D-ribitol as a sole source of carbon and energy.
ribose assimilation assay
Assays for the ability of a microorganism to assimilate ribose as a sole source of carbon and energy.
rutin assimilation assay
Assays for the ability of a microorganism to assimilate rutin as a sole source of carbon and energy.
salicyl alcohol assimilation assay
Assays for the ability of a microorganism to assimilate salicyl alcohol as a sole source of carbon and energy.
sarcosine assimilation assay
Assays for the ability of a microorganism to assimilate sarcosine as a sole source of carbon and energy.
sedoheptulose assimilation assay
Assays for the ability of a microorganism to assimilate sedoheptulose as a sole source of carbon and energy.
selenocysteine assimilation assay
Assays for the ability of a microorganism to assimilate selenocysteine as a sole source of carbon and energy.
serine assimilation assay
Assays for the ability of a microorganism to assimilate serine as a sole source of carbon and energy.
sorbose assimilation assay
Assays for the ability of a microorganism to assimilate sorbose as a sole source of carbon and energy.
spermidine assimilation assay
Assays for the ability of a microorganism to assimilate spermidine as a sole source of carbon and energy.
spermine assimilation assay
Assays for the ability of a microorganism to assimilate spermine as a sole source of carbon and energy.
starch assimilation assay
Assays for the ability of a microorganism to assimilate starch as a sole source of carbon and energy.
sterculic acid assimilation assay
Assays for the ability of a microorganism to assimilate sterculic acid as a sole source of carbon and energy.
syringic acid assimilation assay
Assays for the ability of a microorganism to assimilate syringate (syringic acid) as a sole source of carbon and energy.
tagatose assimilation assay
Assays for the ability of a microorganism to assimilate tagatose as a sole source of carbon and energy.
tartaric acid assimilation assay
Assays for the ability of a microorganism to assimilate tartrate (tartaric acid) as a sole source of carbon and energy.
tetradecanoic acid assimilation assay
Assays for the ability of a microorganism to assimilate tetradecanoate (tetradecanoic acid) as a sole source of carbon and energy.
thioacetamide assimilation assay
Assays for the ability of a microorganism to assimilate thioacetamide as a sole source of carbon and energy.
thiocyanate assimilation assay
Assays for the ability of a microorganism to assimilate thiocyanate as a sole source of carbon and energy.
thioglycolic acid assimilation assay
Assays for the ability of a microorganism to assimilate thioglycolic acid (or thioglycolate) as a sole source of carbon and energy.
threonine assimilation assay
Assays for the ability of a microorganism to assimilate threonine as a sole source of carbon and energy.
threose assimilation assay
Assays for the ability of a microorganism to assimilate threose as a sole source of carbon and energy.
thymine assimilation assay
Assays for the ability of a microorganism to assimilate thymine as a sole source of carbon and energy.
toluene assimilation assay
Assays for the ability of a microorganism to assimilate toluene as a sole source of carbon and energy.
trans-sinapic acid assimilation assay
Assays for the ability of a microorganism to assimilate trans-sinapate (trans-sinapic acid) as a sole source of carbon and energy.
xylene assimilation assay
Assays for the ability of a microorganism to assimilate xylene as a sole source of carbon and energy.
xylan assimilation assay
Assays for the ability of a microorganism to assimilate xylan as a sole source of carbon and energy.
xanthine assimilation assay
Assays for the ability of a microorganism to assimilate xanthine as a sole source of carbon and energy.
veratraldehyde assimilation assay
Assays for the ability of a microorganism to assimilate veratraldehyde as a sole source of carbon and energy.
trans-sinapic acid fermentation assay
Assays for the ability of a microorganism to ferment trans-sinapate (trans-sinapic acid).
trans-aconitic acid fermentation assay
Assays for the ability of a microorganism to ferment trans-aconitic acid (trans-aconitate).
toluene fermentation assay
Assays for the ability of a microorganism to ferment toluene.
thymine fermentation assay
Assays for the ability of a microorganism to ferment thymine.
thymidine 5'-monophosphate fermentation assay
thymidine 5'-monophosphate acidification
Assays for the ability of a microorganism to ferment thymidine 5'-monophosphate.
thymidine fermentation assay
Assays for the ability of a microorganism to ferment thymidine.
threose fermentation assay
Assays for the ability of a microorganism to ferment threose.
L-threonine fermentation assay
Assays for the ability of a microorganism to ferment L-threonine.
threonine fermentation assay
Assays for the ability of a microorganism to ferment threonine.
thioglycolic acid fermentation assay
Assays for the ability of a microorganism to ferment thioglycolic acid (thioglycolate).
thiocyanate fermentation assay
Assays for the ability of a microorganism to ferment thiocyanate.
thioacetamide fermentation assay
Assays for the ability of a microorganism to ferment thioacetamide.
tetradecanoic acid fermentation assay
Assays for the ability of a microorganism to ferment tetradecanoate (tetradecanoic acid).
tartaric acid fermentation assay
Assays for the ability of a microorganism to ferment tartrate (tartaric acid).
tagatose fermentation assay
Assays for the ability of a microorganism to ferment tagatose.
syringic acid fermentation assay
Assays for the ability of a microorganism to ferment syringate (syringic acid).
succinic acid fermentation assay
Assays for the ability of a microorganism to ferment succinate (succinic acid).
succinamic acid fermentation assay
Assays for the ability of a microorganism to ferment succinamic acid.
suberic acid fermentation assay
Assays for the ability of a microorganism to ferment suberate (suberic acid).
sterculic acid fermentation assay
Assays for the ability of a microorganism to ferment sterculic acid.
stachyose fermentation assay
Assays for the ability of a microorganism to ferment stachyose.
spermine fermentation assay
Assays for the ability of a microorganism to ferment spermine.
spermidine fermentation assay
Assays for the ability of a microorganism to ferment spermidine.
sorbose fermentation assay
Assays for the ability of a microorganism to ferment sorbose.
D-serine fermentation assay
Assays for the ability of a microorganism to ferment D-serine.
L-serine fermentation assay
Assays for the ability of a microorganism to ferment L-serine.
serine fermentation assay
Assays for the ability of a microorganism to ferment serine.
selenocysteine fermentation assay
Assays for the ability of a microorganism to ferment selenocysteine.
sedoheptulose fermentation assay
Assays for the ability of a microorganism to ferment sedoheptulose.
sedoheptulosan fermentation assay
Assays for the ability of a microorganism to ferment sedoheptulosan.
sebacic acid fermentation assay
Assays for the ability of a microorganism to ferment sebacate (sebacic acid).
sarcosine fermentation assay
Assays for the ability of a microorganism to ferment sarcosine.
glycerol phosphate fermentation assay
Assays for the ability of a microorganism to ferment glycerol phosphate.
tributyrin fermentation assay
Assays for the ability of a microorganism to ferment tributyrin.
tricarballylic acid fermentation assay
Assays for the ability of a microorganism to ferment tricarballylate (tricarballylic acid).
trihydroxybenzoic acid fermentation assay
Assays for the ability of a microorganism to ferment trihydroxybenzoate (trihydroxybenzoic acid).
trimethylamine fermentation assay
Assays for the ability of a microorganism to ferment trimethylamine.
tryptamine fermentation assay
Assays for the ability of a microorganism to ferment tryptamine.
tryptophan fermentation assay
Assays for the ability of a microorganism to ferment tryptophan.
L-tryptophan fermentation assay
Assays for the ability of a microorganism to ferment L-tryptophan.
tyrosine fermentation assay
Assays for the ability of a microorganism to ferment tyrosine.
L-tyrosine fermentation assay
Assays for the ability of a microorganism to ferment L-tyrosine.
uracil fermentation assay
Assays for the ability of a microorganism to ferment uracil.
urea fermentation assay
Assays for the ability of a microorganism to ferment urea.
uric acid fermentation assay
Assays for the ability of a microorganism to ferment uric acid (urate).
uridine fermentation assay
Assays for the ability of a microorganism to ferment uridine.
UMP fermentation assay
Assays for the ability of a microorganism to ferment UMP.
urocanic acid fermentation assay
Assays for the ability of a microorganism to ferment urocanate (urocanic acid).
valeric acid fermentation assay
Assays for the ability of a microorganism to ferment valerate (valeric acid).
valine fermentation assay
Assays for the ability of a microorganism to ferment valine.
L-valine fermentation assay
Assays for the ability of a microorganism to ferment L-valine.
vanillic acid fermentation assay
Assays for the ability of a microorganism to ferment vanillate (vanillic acid).
vanillin fermentation assay
Assays for the ability of a microorganism to ferment vanillin.
veratraldehyde fermentation assay
Assays for the ability of a microorganism to ferment veratraldehyde.
xanthine fermentation assay
Assays for the ability of a microorganism to ferment xanthine.
methyl mannoside fermentation assay
Assays for the ability of a microorganism to ferment methyl mannoside.
vanillin assimilation assay
Assays for the ability of a microorganism to assimilate vanillin as a sole source of carbon and energy.
vanillic acid assimilation assay
Assays for the ability of a microorganism to assimilate vanillate (vanillic acid) as a sole source of carbon and energy.
valine assimilation assay
Assays for the ability of a microorganism to assimilate valine as a sole source of carbon and energy.
uric acid assimilation assay
Assays for the ability of a microorganism to assimilate uric acid (urate) as a sole source of carbon and energy.
urea assimilation assay
Assays for the ability of a microorganism to assimilate urea as a sole source of carbon and energy.
uracil assimilation assay
Assays for the ability of a microorganism to assimilate uracil as a sole source of carbon and energy.
L-tyrosine assimilation assay
Assays for the ability of a microorganism to assimilate L-tyrosine as a sole source of carbon and energy.
tyrosine assimilation assay
Assays for the ability of a microorganism to assimilate tyrosine as a sole source of carbon and energy.
L-tryptophan assimilation assay
Assays for the ability of a microorganism to assimilate L-tryptophan as a sole source of carbon and energy.
tryptophan assimilation assay
Assays for the ability of a microorganism to assimilate tryptophan as a sole source of carbon and energy.
tryptamine assimilation assay
Assays for the ability of a microorganism to assimilate tryptamine as a sole source of carbon and energy.
trimethylamine assimilation assay
Assays for the ability of a microorganism to assimilate trimethylamine as a sole source of carbon and energy.
trihydroxybenzoic acid assimilation assay
Assays for the ability of a microorganism to assimilate trihydroxybenzoate (trihydroxybenzoic acid) as a sole source of carbon and energy.
tricarballylic acid assimilation assay
Assays for the ability of a microorganism to assimilate tricarballylate (tricarballylic acid) as a sole source of carbon and energy.
tributyrin assimilation assay
Assays for the ability of a microorganism to assimilate tributyrin as a sole source of carbon and energy.
trehalose assimilation assay
Assays for the ability of a microorganism to assimilate trehalose as a sole source of carbon and energy.
xylose assimilation assay
Assays for the ability of a microorganism to assimilate xylose as a sole source of carbon and energy.
methyl mannoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl mannoside as a sole source of carbon and energy.
methyl galactoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl galactoside as a sole source of carbon and energy.
N-acetylgalactosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetylgalactosamine as a sole source of carbon and energy.
N-acetylmannosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetylmannosamine as a sole source of carbon and energy.
N-acetyl-D-glucosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetyl-D-glucosamine as a sole source of carbon and energy.
N-acetyl-D-galactosamine assimilation assay
Assays for the ability of a microorganism to assimilate N-acetyl-D-galactosamine as a sole source of carbon and energy.
alpha-D-glucose assimilation assay
Assays for the ability of a microorganism to assimilate alpha-D-glucose as a sole source of carbon and energy.
xylene fermentation assay
Assays for the ability of a microorganism to ferment xylene.
xylooligosaccharide fermentation assay
Assays for the ability of a microorganism to ferment xylooligosaccharide.
xylose fermentation assay
Assays for the ability of a microorganism to ferment xylose.
methyl galactoside fermentation assay
Assays for the ability of a microorganism to ferment methyl galactoside.
N-acetylgalactosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetylgalactosamine.
N-acetylmannosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetylmannosamine.
N-acetyl-D-glucosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetyl-D-glucosamine.
N-acetyl-D-galactosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetyl-D-galactosamine.
L-mannitol fermentation assay
Assays for the ability of a microorganism to ferment L-mannitol.
alpha-D-mannoside fermentation assay
Assays for the ability of a microorganism to ferment alpha-D-mannoside.
glycerol monophosphate fermentation assay
Assays for the ability of a microorganism to ferment glycerol monophosphate.
D-galactono-1,4-lactone fermentation assay
Assays for the ability of a microorganism to ferment D-galactono-1,4-lactone.
glucuronolactone fermentation assay
Assays for the ability of a microorganism to ferment glucuronolactone.
alpha-D-glucose fermentation assay
Assays for the ability of a microorganism to ferment alpha-D-glucose.
L-arabinitol fermentation assay
trans-caffeic acid assimilation assay
Assays for the ability of a microorganism to assimilate trans-caffeate as a sole source of carbon and energy.
cis-caffeic acid assimilation assay
Assays for the ability of a microorganism to assimilate cis-caffeate as a sole source of carbon and energy.
D-arabinose assimilation assay
Assays for the ability of a microorganism to assimilate D-arabinose as a sole source of carbon and energy.
L-arabinitol assimilation assay
Assays for the ability of a microorganism to assimilate L-arabinitol as a sole source of carbon and energy.
alpha-D-glucose 6-phosphate assimilation assay
alpha-D-glucose 6-phosphate assimilation
Assays for the ability of a microorganism to assimilate alpha-D-glucose 6-phosphate as a sole source of carbon and energy.
(R)-lactic acid assimilation assay
Assays for the ability of a microorganism to assimilate (R)-lactate as a sole source of carbon and energy.
L-lyxose assimilation assay
Assays for the ability of a microorganism to assimilate L-lyxose as a sole source of carbon and energy.
L-mannitol assimilation assay
Assays for the ability of a microorganism to assimilate L-mannitol as a sole source of carbon and energy.
alpha-D-mannoside assimilation assay
Assays for the ability of a microorganism to assimilate alpha-D-mannoside as a sole source of carbon and energy.
glycerol monophosphate assimilation assay
Assays for the ability of a microorganism to assimilate glycerol monophosphate as a sole source of carbon and energy.
D-galacturonic acid assimilation assay
Assays for the ability of a microorganism to assimilate D-galacturonic acid (D-galacturonate) as a sole source of carbon and energy.
L-glucitol assimilation assay
Assays for the ability of a microorganism to assimilate L-glucitol as a sole source of carbon and energy.
D-glucosamine assimilation assay
Assays for the ability of a microorganism to assimilate D-glucosamine as a sole source of carbon and energy.
beta-D-glucosamine assimilation assay
Assays for the ability of a microorganism to assimilate beta-D-glucosamine as a sole source of carbon and energy.
alpha-glucoside assimilation assay
Assays for the ability of a microorganism to assimilate alpha-glucoside as a sole source of carbon and energy.
beta-glucoside assimilation assay
Assays for the ability of a microorganism to assimilate beta-glucoside as a sole source of carbon and energy.
glucuronolactone assimilation assay
Assays for the ability of a microorganism to assimilate glucuronolactone as a sole source of carbon and energy.
D-galactono-1,5-lactone fermentation assay
Assays for the ability of a microorganism to ferment D-galactono-1,5-lactone.
monounsaturated fatty acid fermentation assay
Assays for the ability of a microorganism to ferment monounsaturated fatty acid.
monounsaturated fatty acid acidification
beta-D-fucose fermentation assay
Assays for the ability of a microorganism to ferment beta-D-fucose.
beta-L-fucose fermentation assay
Assays for the ability of a microorganism to ferment beta-L-fucose.
alpha-L-fucose fermentation assay
Assays for the ability of a microorganism to ferment alpha-L-fucose.
D-galacturonic acid fermentation assay
Assays for the ability of a microorganism to ferment D-galacturonic acid (D-galacturonate).
L-glucitol fermentation assay
Assays for the ability of a microorganism to ferment L-glucitol.
D-glucosamine fermentation assay
Assays for the ability of a microorganism to ferment D-glucosamine.
beta-D-glucosamine fermentation assay
Assays for the ability of a microorganism to ferment beta-D-glucosamine.
alpha-glucoside fermentation assay
Assays for the ability of a microorganism to ferment alpha-glucoside.
beta-glucoside fermentation assay
Assays for the ability of a microorganism to ferment beta-glucoside.
fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate fatty acid as a sole source of carbon and energy.
L-citrulline assimilation assay
Assays for the ability of a microorganism to assimilate L-citrulline as a sole source of carbon and energy.
beta-D-fructofuranose assimilation assay
Assays for the ability of a microorganism to assimilate beta-D-fructofuranose as a sole source of carbon and energy.
alpha-D-fructofuranose assimilation assay
Assays for the ability of a microorganism to assimilate alpha-D-fructofuranose as a sole source of carbon and energy.
L-fructose assimilation assay
Assays for the ability of a microorganism to assimilate L-fructose as a sole source of carbon and energy.
beta-D-fucose assimilation assay
Assays for the ability of a microorganism to assimilate beta-D-fucose as a sole source of carbon and energy.
beta-L-fucose assimilation assay
Assays for the ability of a microorganism to assimilate beta-L-fucose as a sole source of carbon and energy.
alpha-L-fucose assimilation assay
Assays for the ability of a microorganism to assimilate alpha-L-fucose as a sole source of carbon and energy.
D-galactono-1,5-lactone assimilation assay
Assays for the ability of a microorganism to assimilate D-galactono-1,5-lactone as a sole source of carbon and energy.
D-galactono-1,4-lactone assimilation assay
Assays for the ability of a microorganism to assimilate D-galactono-1,4-lactone as a sole source of carbon and energy.
L-fructose fermentation assay
Assays for the ability of a microorganism to ferment L-fructose.
alpha-D-fructofuranose fermentation assay
Assays for the ability of a microorganism to ferment alpha-D-fructofuranose.
beta-D-fructofuranose fermentation assay
Assays for the ability of a microorganism to ferment beta-D-fructofuranose.
L-citrulline fermentation assay
Assays for the ability of a microorganism to ferment L-citrulline.
creatinine fermentation assay
Assays for the ability of a microorganism to ferment creatinine.
long-chain fatty acid fermentation assay
Assays for the ability of a microorganism to ferment long-chain fatty acid.
branched-chain fatty acid fermentation assay
Assays for the ability of a microorganism to ferment branched-chain fatty acid.
hydroxy fatty acid fermentation assay
Assays for the ability of a microorganism to ferment hydroxy fatty acid.
medium-chain fatty acid fermentation assay
Assays for the ability of a microorganism to ferment medium-chain fatty acid.
short-chain fatty acid fermentation assay
Assays for the ability of a microorganism to ferment short-chain fatty acid.
straight-chain fatty acid fermentation assay
Assays for the ability of a microorganism to ferment straight-chain fatty acid.
unsaturated fatty acid fermentation assay
Assays for the ability of a microorganism to ferment unsaturated fatty acid.
fatty acid fermentation assay
Assays for the ability of a microorganism to ferment fatty acid.
methyl-branched fatty acid fermentation assay
methyl-branched fatty acid acidification
Assays for the ability of a microorganism to ferment methyl-branched fatty acid.
cyclic fatty acid fermentation assay
Assays for the ability of a microorganism to ferment cyclic fatty acid.
cyclopropeny fatty acid fermentation assay
Assays for the ability of a microorganism to ferment cyclopropeny fatty acid.
nitrogen-containing fatty acid fermentation assay
nitrogen-containing fatty acid fermentation
nitrogen-containing fatty acid acidification
Assays for the ability of a microorganism to ferment nitrogen-containing fatty acid.
L-asparagine fermentation assay
Assays for the ability of a microorganism to ferment L-asparagine.
unsaturated fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate unsaturated fatty acid as a sole source of carbon and energy.
straight-chain fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate straight-chain fatty acid as a sole source of carbon and energy.
short-chain fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate short-chain fatty acid as a sole source of carbon and energy.
medium-chain fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate medium-chain fatty acid as a sole source of carbon and energy.
hydroxy fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate hydroxy fatty acid as a sole source of carbon and energy.
branched-chain fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate branched-chain fatty acid as a sole source of carbon and energy.
long-chain fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate long-chain fatty acid as a sole source of carbon and energy.
monounsaturated fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate monounsaturated fatty acid as a sole source of carbon and energy.
methyl-branched fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate methyl-branched fatty acid as a sole source of carbon and energy.
cyclic fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate cyclic fatty acid as a sole source of carbon and energy.
cyclopropeny fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate cyclopropeny fatty acid as a sole source of carbon and energy.
nitrogen-containing fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate nitrogen-containing fatty acid as a sole source of carbon and energy.
nitrogen-containing fatty acid assimilation
oxo fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate oxo fatty acid as a sole source of carbon and energy.
saturated fatty acid assimilation assay
Assays for the ability of a microorganism to assimilate saturated fatty acid as a sole source of carbon and energy.
methylbutyric acid assimilation assay
Assays for the ability of a microorganism to assimilate methylbutyric acid as a sole source of carbon and energy.
lipoic acid assimilation assay
Assays for the ability of a microorganism to assimilate lipoic acid as a sole source of carbon and energy.
straight-chain saturated fatty acid assimilation assay
straight-chain saturated fatty acid assimilation
Assays for the ability of a microorganism to assimilate straight-chain saturated fatty acid as a sole source of carbon and energy.
oxo fatty acid fermentation assay
Assays for the ability of a microorganism to ferment oxo fatty acid.
saturated fatty acid fermentation assay
Assays for the ability of a microorganism to ferment saturated fatty acid.
methylbutyric acid fermentation assay
Assays for the ability of a microorganism to ferment methylbutyric acid.
lipoic acid fermentation assay
Assays for the ability of a microorganism to ferment lipoic acid.
carbohydrate fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate.
alditol fermentation assay
Assays for the ability of a microorganism to ferment alditol.
hexitol fermentation assay
Assays for the ability of a microorganism to ferment hexitol.
pentitol fermentation assay
Assays for the ability of a microorganism to ferment pentitol.
tetritol fermentation assay
Assays for the ability of a microorganism to ferment tetritol.
polysaccharide derivative fermentation assay
Assays for the ability of a microorganism to ferment polysaccharide derivative.
methyl glycoside fermentation assay
Assays for the ability of a microorganism to ferment methyl glycoside.
straight-chain saturated fatty acid fermentation assay
straight-chain saturated fatty acid fermentation
Assays for the ability of a microorganism to ferment straight-chain saturated fatty acid.
straight-chain saturated fatty acid acidification
carbohydrate assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate as a sole source of carbon and energy.
alditol assimilation assay
Assays for the ability of a microorganism to assimilate alditol as a sole source of carbon and energy.
hexitol assimilation assay
Assays for the ability of a microorganism to assimilate hexitol as a sole source of carbon and energy.
pentitol assimilation assay
Assays for the ability of a microorganism to assimilate pentitol as a sole source of carbon and energy.
tetritol assimilation assay
Assays for the ability of a microorganism to assimilate tetritol as a sole source of carbon and energy.
aromatic ether assimilation assay
Assays for the ability of a microorganism to assimilate aromatic ether as a sole source of carbon and energy.
dimethoxybenzene assimilation assay
Assays for the ability of a microorganism to assimilate dimethoxybenzene as a sole source of carbon and energy.
monomethoxybenzene assimilation assay
Assays for the ability of a microorganism to assimilate monomethoxybenzene as a sole source of carbon and energy.
carbohydrate acid assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate acid as a sole source of carbon and energy.
aldaric acid assimilation assay
Assays for the ability of a microorganism to assimilate aldaric acid as a sole source of carbon and energy.
soluble starch fermentation assay
Assays for the ability of a microorganism to ferment soluble starch.
polysaccharide fermentation assay
Assays for the ability of a microorganism to ferment polysaccharide.
trisaccharide fermentation assay
Assays for the ability of a microorganism to ferment trisaccharide.
raffinose family oligosaccharide fermentation assay
Assays for the ability of a microorganism to ferment raffinose family oligosaccharide.
raffinose family oligosaccharide fermentation
raffinose family oligosaccharide acidification
maltooligosaccharide fermentation assay
Assays for the ability of a microorganism to ferment maltooligosaccharide.
disaccharide fermentation assay
Assays for the ability of a microorganism to ferment disaccharide.
cyclic oligosaccharide fermentation assay
Assays for the ability of a microorganism to ferment cyclic oligosaccharide.
oligosaccharide fermentation assay
Assays for the ability of a microorganism to ferment oligosaccharide.
diketoaldonic acid fermentation assay
Assays for the ability of a microorganism to ferment diketoaldonic acid.
deoxy sugar fermentation assay
Assays for the ability of a microorganism to ferment deoxy sugar.
ketoaldonic acid fermentation assay
Assays for the ability of a microorganism to ferment ketoaldonic acid.
uronic acid fermentation assay
Assays for the ability of a microorganism to ferment uronic acid.
hexaric acid fermentation assay
Assays for the ability of a microorganism to ferment hexaric acid.
tetraric acid fermentation assay
Assays for the ability of a microorganism to ferment tetraric acid.
hexonic acid fermentation assay
Assays for the ability of a microorganism to ferment hexonic acid.
aldonic acid fermentation assay
Assays for the ability of a microorganism to ferment aldonic acid.
aldaric acid fermentation assay
Assays for the ability of a microorganism to ferment aldaric acid.
carbohydrate acid fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate acid.
monomethoxybenzene fermentation assay
Assays for the ability of a microorganism to ferment monomethoxybenzene.
dimethoxybenzene fermentation assay
Assays for the ability of a microorganism to ferment dimethoxybenzene.
aromatic ether fermentation assay
Assays for the ability of a microorganism to ferment aromatic ether.
aldonic acid assimilation assay
Assays for the ability of a microorganism to assimilate aldonic acid as a sole source of carbon and energy.
hexonic acid assimilation assay
Assays for the ability of a microorganism to assimilate hexonic acid as a sole source of carbon and energy.
tetraric acid assimilation assay
Assays for the ability of a microorganism to assimilate tetraric acid as a sole source of carbon and energy.
hexaric acid assimilation assay
Assays for the ability of a microorganism to assimilate hexaric acid as a sole source of carbon and energy.
uronic acid assimilation assay
Assays for the ability of a microorganism to assimilate uronic acid as a sole source of carbon and energy.
ketoaldonic acid assimilation assay
Assays for the ability of a microorganism to assimilate ketoaldonic acid as a sole source of carbon and energy.
deoxy sugar assimilation assay
Assays for the ability of a microorganism to assimilate deoxy sugar as a sole source of carbon and energy.
diketoaldonic acid assimilation assay
Assays for the ability of a microorganism to assimilate diketoaldonic acid as a sole source of carbon and energy.
oligosaccharide assimilation assay
Assays for the ability of a microorganism to assimilate oligosaccharide as a sole source of carbon and energy.
cyclic oligosaccharide assimilation assay
Assays for the ability of a microorganism to assimilate cyclic oligosaccharide as a sole source of carbon and energy.
disaccharide assimilation assay
Assays for the ability of a microorganism to assimilate disaccharide as a sole source of carbon and energy.
maltooligosaccharide assimilation assay
Assays for the ability of a microorganism to assimilate maltooligosaccharide as a sole source of carbon and energy.
raffinose family oligosaccharide assimilation assay
raffinose family oligosaccharide assimilation
Assays for the ability of a microorganism to assimilate raffinose family oligosaccharide as a sole source of carbon and energy.
trisaccharide assimilation assay
Assays for the ability of a microorganism to assimilate trisaccharide as a sole source of carbon and energy.
polysaccharide assimilation assay
Assays for the ability of a microorganism to assimilate polysaccharide as a sole source of carbon and energy.
inulobiose assimilation assay
Assays for the ability of a microorganism to assimilate inulobiose as a sole source of carbon and energy.
soluble starch assimilation assay
Assays for the ability of a microorganism to assimilate soluble starch as a sole source of carbon and energy.
monosaccharide assimilation assay
Assays for the ability of a microorganism to assimilate monosaccharide as a sole source of carbon and energy.
aldohexose assimilation assay
Assays for the ability of a microorganism to assimilate aldohexose as a sole source of carbon and energy.
aldose assimilation assay
Assays for the ability of a microorganism to assimilate aldose as a sole source of carbon and energy.
aldotetrose assimilation assay
Assays for the ability of a microorganism to assimilate aldotetrose as a sole source of carbon and energy.
ahydro sugar assimilation assay
Assays for the ability of a microorganism to assimilate ahydro sugar as a sole source of carbon and energy.
hexose assimilation assay
Assays for the ability of a microorganism to assimilate hexose as a sole source of carbon and energy.
pentose assimilation assay
Assays for the ability of a microorganism to assimilate pentose as a sole source of carbon and energy.
carbohydrate derivative assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate derivative as a sole source of carbon and energy.
alditol phosphate assimilation assay
Assays for the ability of a microorganism to assimilate alditol phosphate as a sole source of carbon and energy.
amino sugar assimilation assay
Assays for the ability of a microorganism to assimilate amino sugar as a sole source of carbon and energy.
carbohydrate acid derivative assimilation assay
carbohydrate acid derivative assimilation
Assays for the ability of a microorganism to assimilate carbohydrate acid derivative as a sole source of carbon and energy.
carbohydrate lactone assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate lactone as a sole source of carbon and energy.
carbohydrate phosphate assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate phosphate as a sole source of carbon and energy.
glycoside assimilation assay
Assays for the ability of a microorganism to assimilate glycoside as a sole source of carbon and energy.
disaccharide derivative assimilation assay
Assays for the ability of a microorganism to assimilate disaccharide derivative as a sole source of carbon and energy.
monosaccharide derivative assimilation assay
Assays for the ability of a microorganism to assimilate monosaccharide derivative as a sole source of carbon and energy.
disaccharide derivative fermentation assay
Assays for the ability of a microorganism to ferment disaccharide derivative.
glycoside fermentation assay
Assays for the ability of a microorganism to ferment glycoside.
carbohydrate phosphate fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate phosphate.
carbohydrate lactone fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate lactone.
carbohydrate acid derivative fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate acid derivative.
carbohydrate acid derivative acidification
carbohydrate acid derivative fermentation
amino sugar fermentation assay
Assays for the ability of a microorganism to ferment amino sugar.
alditol phosphate fermentation assay
Assays for the ability of a microorganism to ferment alditol phosphate.
carbohydrate derivative fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate derivative.
pentose fermentation assay
Assays for the ability of a microorganism to ferment pentose.
hexose fermentation assay
Assays for the ability of a microorganism to ferment hexose.
ahydro sugar fermentation assay
Assays for the ability of a microorganism to ferment ahydro sugar.
aldotetrose fermentation assay
Assays for the ability of a microorganism to ferment aldotetrose.
aldose fermentation assay
Assays for the ability of a microorganism to ferment aldose.
aldohexose fermentation assay
Assays for the ability of a microorganism to ferment aldohexose.
monosaccharide fermentation assay
Assays for the ability of a microorganism to ferment monosaccharide.
methyl glycoside assimilation assay
Assays for the ability of a microorganism to assimilate methyl glycoside as a sole source of carbon and energy.
hexoside assimilation assay
Assays for the ability of a microorganism to assimilate hexoside as a sole source of carbon and energy.
galactoside assimilation assay
Assays for the ability of a microorganism to assimilate galactoside as a sole source of carbon and energy.
polysaccharide derivative assimilation assay
Assays for the ability of a microorganism to assimilate polysaccharide derivative as a sole source of carbon and energy.
aminoglycan assimilation assay
Assays for the ability of a microorganism to assimilate aminoglycan as a sole source of carbon and energy.
carbohydrate sulfate assimilation assay
Assays for the ability of a microorganism to assimilate carbohydrate sulfate as a sole source of carbon and energy.
nucleobase assimilation assay
Assays for the ability of a microorganism to assimilate nucleobase as a sole source of carbon and energy.
quinolines assimilation assay
Assays for the ability of a microorganism to assimilate quinolines as a sole source of carbon and energy.
proteinogenic amino acid assimilation assay
Assays for the ability of a microorganism to assimilate proteinogenic amino acid as a sole source of carbon and energy.
organonitrogen compound assimilation assay
Assays for the ability of a microorganism to assimilate organonitrogen compound as a sole source of carbon and energy.
monosaccharide derivative fermentation assay
Assays for the ability of a microorganism to ferment monosaccharide derivative.
aminoglycan fermentation assay
Assays for the ability of a microorganism to ferment aminoglycan.
carbohydrate sulfate fermentation assay
Assays for the ability of a microorganism to ferment carbohydrate sulfate.
organonitrogen compound fermentation assay
Assays for the ability of a microorganism to ferment organonitrogen compound.
proteinogenic amino acid fermentation assay
Assays for the ability of a microorganism to ferment proteinogenic amino acid.
quinolines fermentation assay
Assays for the ability of a microorganism to ferment quinolines.
nucleobase fermentation assay
Assays for the ability of a microorganism to ferment nucleobase.
lactam fermentation assay
Assays for the ability of a microorganism to ferment lactam.
galactoside fermentation assay
Assays for the ability of a microorganism to ferment galactoside.
hexoside fermentation assay
Assays for the ability of a microorganism to ferment hexoside.
lactam assimilation assay
Assays for the ability of a microorganism to assimilate lactam as a sole source of carbon and energy.
pyrimidines assimilation assay
Assays for the ability of a microorganism to assimilate pyrimidines as a sole source of carbon and energy.
amino acid assimilation assay
Assays for the ability of a microorganism to assimilate amino acid as a sole source of carbon and energy.
carboxylic acid assimilation assay
Assays for the ability of a microorganism to assimilate carboxylic acid as a sole source of carbon and energy.
organosulfur compound assimilation assay
Assays for the ability of a microorganism to assimilate organosulfur compound as a sole source of carbon and energy.
organochlorine compound assimilation assay
Assays for the ability of a microorganism to assimilate organochlorine compound as a sole source of carbon and energy.
benzenes assimilation assay
Assays for the ability of a microorganism to assimilate benzenes as a sole source of carbon and energy.
phenols assimilation assay
Assays for the ability of a microorganism to assimilate phenols as a sole source of carbon and energy.
cinnamic acids assimilation assay
Assays for the ability of a microorganism to assimilate cinnamic acids as a sole source of carbon and energy.
polyol assimilation assay
Assays for the ability of a microorganism to assimilate polyol as a sole source of carbon and energy.
alcohol assimilation assay
Assays for the ability of a microorganism to assimilate alcohol as a sole source of carbon and energy.
lipid assimilation assay
Assays for the ability of a microorganism to assimilate lipid as a sole source of carbon and energy.
benzoic acids assimilation assay
Assays for the ability of a microorganism to assimilate benzoic acids as a sole source of carbon and energy.
amino acid derivative assimilation assay
Assays for the ability of a microorganism to assimilate amino acid derivative as a sole source of carbon and energy.
carboxamide assimilation assay
Assays for the ability of a microorganism to assimilate carboxamide as a sole source of carbon and energy.
pyrimidines fermentation assay
Assays for the ability of a microorganism to ferment pyrimidines.
amino acid fermentation assay
Assays for the ability of a microorganism to ferment amino acid.
carboxylic acid fermentation assay
Assays for the ability of a microorganism to ferment carboxylic acid.
ketone fermentation assay
Assays for the ability of a microorganism to ferment ketone.
exopolysaccharide fermentation assay
Assays for the ability of a microorganism to ferment exopolysaccharide.
biomacromolecule fermentation assay
Assays for the ability of a microorganism to ferment biomacromolecule.
information biomacromolecule fermentation assay
information biomacromolecule fermentation
Assays for the ability of a microorganism to ferment information biomacromolecule.
information biomacromolecule acidification
phenylethylamine fermentation assay
Assays for the ability of a microorganism to ferment phenylethylamine.
organic amino compound fermentation assay
Assays for the ability of a microorganism to ferment organic amino compound.
amine fermentation assay
Assays for the ability of a microorganism to ferment amine.
peptide fermentation assay
Assays for the ability of a microorganism to ferment peptide.
carboxamide fermentation assay
Assays for the ability of a microorganism to ferment carboxamide.
amino acid derivative fermentation assay
Assays for the ability of a microorganism to ferment amino acid derivative.
benzoic acids fermentation assay
Assays for the ability of a microorganism to ferment benzoic acids.
lipid fermentation assay
Assays for the ability of a microorganism to ferment lipid.
alcohol fermentation assay
Assays for the ability of a microorganism to ferment alcohol.
polyol fermentation assay
Assays for the ability of a microorganism to ferment polyol.
cinnamic acids fermentation assay
Assays for the ability of a microorganism to ferment cinnamic acids.
phenols fermentation assay
Assays for the ability of a microorganism to ferment phenols.
benzenes fermentation assay
Assays for the ability of a microorganism to ferment benzenes.
organochlorine compound fermentation assay
Assays for the ability of a microorganism to ferment organochlorine compound.
organosulfur compound fermentation assay
Assays for the ability of a microorganism to ferment organosulfur compound.
peptide assimilation assay
Assays for the ability of a microorganism to assimilate peptide as a sole source of carbon and energy.
D-tartaric acid assimilation assay
Assays for the ability of a microorganism to assimilate D-tartaric acid (D-tartrate) as a sole source of carbon and energy.
talose assimilation assay
Assays for the ability of a microorganism to assimilate talose as a sole source of carbon and energy.
D-talose assimilation assay
Assays for the ability of a microorganism to assimilate D-talose as a sole source of carbon and energy.
D-sorbose assimilation assay
Assays for the ability of a microorganism to assimilate D-sorbose as a sole source of carbon and energy.
L-ribose assimilation assay
Assays for the ability of a microorganism to assimilate L-ribose as a sole source of carbon and energy.
alpha-L-rhamnopyranose assimilation assay
Assays for the ability of a microorganism to assimilate alpha-L-rhamnopyranose as a sole source of carbon and energy.
methylamines assimilation assay
Assays for the ability of a microorganism to assimilate methylamines as a sole source of carbon and energy.
amine assimilation assay
Assays for the ability of a microorganism to assimilate amine as a sole source of carbon and energy.
organic amino compound assimilation assay
Assays for the ability of a microorganism to assimilate organic amino compound as a sole source of carbon and energy.
D-rhamnose assimilation assay
Assays for the ability of a microorganism to assimilate D-rhamnose as a sole source of carbon and energy.
information biomacromolecule assimilation assay
information biomacromolecule assimilation
Assays for the ability of a microorganism to assimilate information biomacromolecule as a sole source of carbon and energy.
biomacromolecule assimilation assay
Assays for the ability of a microorganism to assimilate biomacromolecule as a sole source of carbon and energy.
exopolysaccharide assimilation assay
Assays for the ability of a microorganism to assimilate exopolysaccharide as a sole source of carbon and energy.
ketone assimilation assay
Assays for the ability of a microorganism to assimilate ketone as a sole source of carbon and energy.
methylamines fermentation assay
Assays for the ability of a microorganism to ferment methylamines.
alpha-L-rhamnopyranose fermentation assay
Assays for the ability of a microorganism to ferment alpha-L-rhamnopyranose.
D-rhamnose fermentation assay
Assays for the ability of a microorganism to ferment D-rhamnose.
N-acetyl-D-mannosamine fermentation assay
Assays for the ability of a microorganism to ferment N-acetyl-D-mannosamine.
mixture fermentation assay
Assays for the ability of a microorganism to ferment mixture.
mixture metabolic assay
Assays for the ability of a microorganize to metabolize a mixture.
D-threonine fermentation assay
Assays for the ability of a microorganism to ferment D-threonine.
L-tartartic acid fermentation assay
Assays for the ability of a microorganism to ferment L-tartartic acid (L-tartrate).
D-tartaric acid fermentation assay
Assays for the ability of a microorganism to ferment D-tartaric acid (D-tartrate).
talose fermentation assay
Assays for the ability of a microorganism to ferment talose.
D-talose fermentation assay
Assays for the ability of a microorganism to ferment D-talose.
D-sorbose fermentation assay
Assays for the ability of a microorganism to ferment D-sorbose.
L-ribose fermentation assay
Assays for the ability of a microorganism to ferment L-ribose.
L-tartartic acid assimilation assay
Assays for the ability of a microorganism to assimilate L-tartartic acid (L-tartrate) as a sole source of carbon and energy.
D-threonine assimilation assay
Assays for the ability of a microorganism to assimilate D-threonine as a sole source of carbon and energy.
L-xylose assimilation assay
Assays for the ability of a microorganism to assimilate L-xylose as a sole source of carbon and energy.
chemical entity metabolic assay
Assays for the ability of a microorganism to metabolize (oxidize, reduce, or ferment) a chemical entity as a sole source of carbon and/or energy.
mixture assimilation assay
Assays for the ability of a microorganism to assimilate mixture as a sole source of carbon and energy.
undefined organic chemical mixture assimilation assay
Assays for the ability of a microorganism to assimilate undefined organic chemical mixture as a sole source of carbon and energy.
undefined organic chemical mixture assimilation
yeast extract assimilation assay
Assays for the ability of a microorganism to assimilate yeast extract as a sole source of carbon and energy.
tryptone assimilation assay
Assays for the ability of a microorganism to assimilate tryptone as a sole source of carbon and energy.
Trypticase peptone assimilation assay
Assays for the ability of a microorganism to assimilate Trypticase peptone as a sole source of carbon and energy.
soy peptone assimilation assay
Assays for the ability of a microorganism to assimilate soy peptone as a sole source of carbon and energy.
proteose peptone assimilation assay
Assays for the ability of a microorganism to assimilate proteose peptone as a sole source of carbon and energy.
protein assimilation assay
Assays for the ability of a microorganism to assimilate protein as a sole source of carbon and energy.
peptone assimilation assay
Assays for the ability of a microorganism to assimilate peptone as a sole source of carbon and energy.
diol fermentation assay
Assays for the ability of a microorganism to ferment diol.
chopped meat fermentation assay
Assays for the ability of a microorganism to ferment chopped meat.
peptone fermentation assay
Assays for the ability of a microorganism to ferment peptone substrates.
protein fermentation assay
Assays for the ability of a microorganism to ferment protein.
proteose peptone fermentation assay
Assays for the ability of a microorganism to ferment proteose peptone.
soy peptone fermentation assay
Assays for the ability of a microorganism to ferment soy peptone.
Trypticase peptone fermentation assay
Assays for the ability of a microorganism to ferment Trypticase peptone.
tryptone fermentation assay
Assays for the ability of a microorganism to ferment tryptone.
yeast extract fermentation assay
Assays for the ability of a microorganism to ferment yeast extract.
undefined organic chemical mixture fermentation assay
Assays for the ability of a microorganism to ferment undefined organic chemical mixture.
undefined organic chemical mixture fermentation
undefined organic chemical mixture acidification
chopped meat assimilation assay
Assays for the ability of a microorganism to assimilate chopped meat as a sole source of carbon and energy.
diol assimilation assay
Assays for the ability of a microorganism to assimilate diol as a sole source of carbon and energy.
casitone assimilation assay
Assays for the ability of a microorganism to assimilate casitone as a sole source of carbon and energy.
casein hydrolysate assimilation assay
Assays for the ability of a microorganism to assimilate casein hydrolysate as a sole source of carbon and energy.
casein assimilation assay
Assays for the ability of a microorganism to assimilate casein as a sole source of carbon and energy.
benzyl alcohol assimilation assay
Assays for the ability of a microorganism to assimilate benzyl alcohol as a sole source of carbon and energy.
benzene assimilation assay
Assays for the ability of a microorganism to assimilate benzene as a sole source of carbon and energy.
nicotinate fermentation assay
Assays for the ability of a microorganism to ferment nicotinate (nicotinic acid).
orotic acid fermentation assay
Assays for the ability of a microorganism to ferment orotic acid (orotate).
salicylic acid fermentation assay
Assays for the ability of a microorganism to ferment salicylic acid (salicylate).
sorbic acid fermentation assay
Assays for the ability of a microorganism to ferment sorbic acid (sorbate).
alpha-mannosidase assay with BrNaphthol
An assay for the activity of alpha-mannosidase in a microorganism. An assay for alpha-mannosidase activity using 6-bromo-2-naphthyl-alpha-L-mannoside. Alpha-mannosidase will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (a bromo subsituted azo dye) that is purple in color.
casein fermentation assay
Assays for the ability of a microorganism to ferment casein.
casein hydrolysate fermentation assay
Assays for the ability of a microorganism to ferment casein hydrolysate.
casitone fermentation assay
Assays for the ability of a microorganism to ferment casitone.
An assay for the activity of beta-glucuronidase in a microorganism. Uses the substrate resorufin-beta-D-glucuronide. Beta-glucuronidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange.
L-proline arylamidase assay using methoxy-NA
Uses the substrate L-proline 4-methoxy-2-naphthylamide at pH 7.5. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
L-arginine dihydrolase assay
The purpose of this assay is to determine if a microbial isolate is capable of metabolizing arginine under anaerobic conditions. When arginine is metabolized, the pH of the medium increases due to the accumulation of ammonia and organic amines (e.g. putrescine). This turns color of the pH indicator (e.g. bromcresol purple, cresol red) to red/orange. A positive test yields a red/orange color; a negative test is yellow. Arginine dihydrolase (arginine deiminase) catalyzes the reaction: L-arginine + H2O <=> L-citrulline + NH3a
Chymotrypsin assay that uses the substrate N-glutaryl-phenylalanine 2-naphthylamide. Trypsin activity will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is purple in color.
An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase.
An assay for the activity of beta-D-fucosidase in a microorganism using various chromogenic substrates.
An alpha-amino acid arylamidase assay. Proline arylamidase activity (which could be from proline aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing a colored product.
L-arginine arylamidase assay using methoxy-NA
An L-arginine arylamidase assay that uses the substrate L-arginine-4-methoxy-2-naphthylamide (L-arginine-4-methoxy-beta-naphthylamide) at pH 7.5. Arginine arylamidase activity (which could be from arginine aminopeptidase as well as other dipeptidase enzymes) will cleave the substrate, releasing 2-naphthylamide. When reacted with Fast Blue BB it forms a colored insoluble precipitate that is orange in color. A positive reaction is orange; a negative reaction is colorless.
An alpha-amino acid arylamidase assay that assays for L-arginine arylamidase activity.
A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism.
N-acetyl-beta-glucosaminidase assay with BrNaphthol
An assay for the activity of N-acetyl-beta-glucosaminidase in a microorganism. Uses the substrate 6-bromo-2-naphthyl-N-acetyl-beta-D-glucosaminide. N-acetyl-beta-glucosaminidase activity (catalyzing the hydrolysis of terminal beta-N-acetylglucosamine residues from oligosaccharides) will cleave the substrate, producing 6-bromo-2-naphthol. When reacted with Fast Blue BB it forms a colored insoluble precipitate (an azo dye) that is brown in color.
A carbohydrate hydrolysis assay for the activity of beta-mannosidase in a microorganism using the substrate 4-nitrophenyl-beta-D-mannopyranoside. beta-Mannosidase activity (catalyzing the hydrolysis of terminal non-reducing beta-D-mannose residues in beta-D-mannosides) will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
An L-arabinosidase assay for the activity of L-arabinofuranosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal L-arabinofuranoside residues in alpha-L-arabinofuranosides.
A carbohydrate hydrolysis assay for the activity of L-arabinosidase in a microorganism using various chromogenic substrates which carries out the hydrolysis of terminal L-arabinoside residues in L-arabinosides.
gamma glutamyl transferase assay using nitroanilide
An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using L-glutamic acid gamma-(4-nitroanilide). Gamma-glutamyl transferase activity cleaves the substrate, releasing p-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
gamma glutamyl transferase assay using carboxy-nitroanilide
An amino acid arylamidase assay that measures the activity of gamma glutamyl transferase using L-glutamic acid gamma-(3-carboxy-4-nitroanilide). Gamma-glutamyl transferase activity cleaves the substrate, releasing 3-carboxy-4-nitroaniline which is bright yellow in color. A positive test is yellow; a negative test is colorless.
bovine serum albumin assimilation assay
Assays for the ability of a microorganism to assimilate bovine serum albumin as a sole source of carbon and energy.
diphenylamine assimilation assay
Assays for the ability of a microorganism to assimilate diphenylamine as a sole source of carbon and energy.
fibrin assimilation assay
Assays for the ability of a microorganism to assimilate fibrin as a sole source of carbon and energy.
fucoidan assimilation assay
Assays for the ability of a microorganism to assimilate fucoidan as a sole source of carbon and energy.
galactan assimilation assay
Assays for the ability of a microorganism to assimilate galactan as a sole source of carbon and energy.
galactomannan assimilation assay
Assays for the ability of a microorganism to assimilate galactomannan as a sole source of carbon and energy.
methyl-3,4-dimethoxycinnamate assimilation assay
methyl-3,4-dimethoxycinnamate assimilation
Assays for the ability of a microorganism to assimilate methyl-3,4-dimethoxycinnamate (methyl-3,4-dimethoxycinnamic acid) as a sole source of carbon and energy.
naphthalene assimilation assay
Assays for the ability of a microorganism to assimilate naphthalene as a sole source of carbon and energy.
nicotinate assimilation assay
Assays for the ability of a microorganism to assimilate nicotinate (nicotinic acid) as a sole source of carbon and energy.
orotic acid assimilation assay
Assays for the ability of a microorganism to assimilate orotic acid (orotate) as a sole source of carbon and energy.
salicylic acid assimilation assay
Assays for the ability of a microorganism to assimilate salicylic acid (salicylate) as a sole source of carbon and energy.
naphthalene fermentation assay
Assays for the ability of a microorganism to ferment naphthalene.
methyl-3,4-dimethoxycinnamate fermentation assay
methyl-3,4-dimethoxycinnamate fermentation
methyl-3,4-dimethoxycinnamate acidification
Assays for the ability of a microorganism to ferment methyl-3,4-dimethoxycinnamate (methyl-3,4-dimethoxycinnamic acid).
galactomannan fermentation assay
Assays for the ability of a microorganism to ferment galactomannan.
galactan fermentation assay
Assays for the ability of a microorganism to ferment galactan.
fucoidan fermentation assay
Assays for the ability of a microorganism to ferment fucoidan.
fibrin fermentation assay
Assays for the ability of a microorganism to ferment fibrin.
diphenylamine fermentation assay
Assays for the ability of a microorganism to ferment diphenylamine.
bovine serum albumin fermentation assay
Assays for the ability of a microorganism to ferment bovine serum albumin.
benzyl alcohol fermentation assay
Assays for the ability of a microorganism to ferment benzyl alcohol.
benzene fermentation assay
Assays for the ability of a microorganism to ferment benzene.
sorbic acid assimilation assay
Assays for the ability of a microorganism to assimilate sorbic acid (sorbate) as a sole source of carbon and energy.
soya extract assimilation assay
Assays for the ability of a microorganism to assimilate soya extract as a sole source of carbon and energy.
trimethylamine N-oxide assimilation assay
Assays for the ability of a microorganism to assimilate trimethylamine N-oxide as a sole source of carbon and energy.
benzenoid aromatic compound assimilation assay
Assays for the ability of a microorganism to assimilate benzenoid aromatic compound as a sole source of carbon and energy.
benzenoid aromatic compound assimilation
organic aromatic compound assimilation assay
Assays for the ability of a microorganism to assimilate organic aromatic compound as a sole source of carbon and energy.
keratin assimilation assay
Assays for the ability of a microorganism to assimilate keratin as a sole source of carbon and energy.
beta-glucosidase with resorufin
An assay for the activity of beta-glucosidase in a microorganism. Uses the substrate resorufin-beta-D-glucopyranoside. Beta-glucosidase will cleave the substrate, producing resorufin, which is pink. A positive result is fluorescent pink/red-orange; a negative result is orange.
soya extract fermentation assay
Assays for the ability of a microorganism to ferment soya extract.
trimethylamine N-oxide fermentation assay
Assays for the ability of a microorganism to ferment trimethylamine N-oxide.
benzenoid aromatic compound fermentation assay
benzenoid aromatic compound acidification
Assays for the ability of a microorganism to ferment benzenoid aromatic compound.
benzenoid aromatic compound fermentation
organic aromatic compound fermentation assay
Assays for the ability of a microorganism to ferment organic aromatic compound.
alkaline phosphatase assay using pNP-CHA
Alkaline phosphatase assay that uses the substrate 4-nitrophenyl-beta-D-galactopyranoside-2-CHA (para-nitrophenyl-beta-D-galactopyranoside-2-CHA). Under alkaline conditions, alkaline phosphatase will cleave the substrate, producing 4-nitrophenol which is yellow. A positive result is yellow; a negative result is colorless.
keratin fermentation assay
Assays for the ability of a microorganism to ferment keratin.
isovanillin-8
pubchem term is: 3-Hydroxy-4-methoxybenzaldehyde
6-bromo-2-naphthyl alpha-D-mannoside-9
4-nitrophenyl beta-D-fucoside-8
4-nitrophenyl-beta-D-galactopyranoside-2-CHA-8
cyclohexyl ammonium salt, e.g. http://www.melford.co.uk/index.php?sid=396401522&t=sub_pages&cat=39
4-nitroaniline-8
resorufin-beta-D-glucuronide-8
resorufin-beta-D-glucopyranoside-8
resorufin-beta-D-galactopyranoside-8
resorufin-8
N-(gamma-L-glutamyl)-4-methoxy-2-naphthylamide-8
L-proline 4-methoxy-2-naphthylamide-8
L-arginine 4-methoxy-2-naphthylamide-8
6-bromo-2-naphthyl-N-acetyl-beta-D-glucosaminide-8
3-carboxy-4-nitroaniline-8
pubchem term is 5-Amino-2-nitrobenzoic acid
temp2
temp3
temp4
temp5
temp6
temp7
temp8
temp9
L-glutamic acid gamma-(3-carboxy-4-nitroanilide)-8
L-glutamic acid gamma-(4-nitroanilide)-8
L-proline 4-nitroanilide-8
temp13
temp14
temp15
temp16
temp17
temp18
temp19
temp20
Unit of cell width defined as an integer number of cells (i.e. 2 cells' width or 3 cells' width).
Wikipedia: pH In chemistry, pH (/piːˈeɪtʃ/) is a numeric scale used to specify the acidity or alkalinity of an aqueous solution. It is the negative of the logarithm to base 10 of the activity of the hydrogen ion. Solutions with a pH less than 7 are acidic and solutions with a pH greater than 7 are alkaline or basic. Pure water is neutral, being neither an acid nor a base. Contrary to popular belief, the pH value can be less than 0 or greater than 14 for very strong acids and bases respectively.[1]
The log of the hydrogen ion concentration.
Unit of atmospheric pressure at the surface of the Earth.
Micrometers (or microns), a measurement of length, 10^-6 of a meter.
A measurement of concentration of solution in weight per 100 g (w/w), or in weight per 100 mL (w/v).