CellProfiler Pipeline: http://www.cellprofiler.org Version:5 DateRevision:421 GitHash: ModuleCount:9 HasImagePlaneDetails:False LoadData:[module_num:1|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Input data file location:Elsewhere...| Name of the file:None Load images based on this data?:Yes Base image location:Default Input Folder| Process just a range of rows?:No Rows to process:1,100000 Group images by metadata?:No Select metadata tags for grouping: Rescale intensities?:Yes ImageMath:[module_num:2|svn_version:'Unknown'|variable_revision_number:5|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Operation:Add Raise the power of the result by:1.0 Multiply the result by:1.0 Add to result:0.0 Set values less than 0 equal to 0?:Yes Set values greater than 1 equal to 1?:Yes Replace invalid values with 0?:Yes Ignore the image masks?:No Name the output image:Membrane Image or measurement?:Image Select the first image:CD45 Multiply the first image by:1.0 Measurement: Image or measurement?:Image Select the second image:PanKeratin Multiply the second image by:1.0 Measurement: Image or measurement?:Image Select the third image:eCadherin Multiply the third image by:1.0 Measurement: IdentifyPrimaryObjects:[module_num:3|svn_version:'Unknown'|variable_revision_number:15|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:DNA1 Name the primary objects to be identified:Nuclei Typical diameter of objects, in pixel units (Min,Max):5,12 Discard objects outside the diameter range?:No Discard objects touching the border of the image?:No Method to distinguish clumped objects:Intensity Method to draw dividing lines between clumped objects:Intensity Size of smoothing filter:10 Suppress local maxima that are closer than this minimum allowed distance:7.0 Speed up by using lower-resolution image to find local maxima?:Yes Fill holes in identified objects?:After both thresholding and declumping Automatically calculate size of smoothing filter for declumping?:Yes Automatically calculate minimum allowed distance between local maxima?:Yes Handling of objects if excessive number of objects identified:Continue Maximum number of objects:500 Use advanced settings?:Yes Threshold setting version:12 Threshold strategy:Global Thresholding method:Otsu Threshold smoothing scale:1 Threshold correction factor:1.0 Lower and upper bounds on threshold:0.0,1.0 Manual threshold:0.0 Select the measurement to threshold with:None Two-class or three-class thresholding?:Two classes Log transform before thresholding?:No Assign pixels in the middle intensity class to the foreground or the background?:Foreground Size of adaptive window:50 Lower outlier fraction:0.05 Upper outlier fraction:0.05 Averaging method:Mean Variance method:Standard deviation # of deviations:2.0 Thresholding method:Otsu IdentifySecondaryObjects:[module_num:4|svn_version:'Unknown'|variable_revision_number:10|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:True] Select the input objects:Nuclei Name the objects to be identified:Cells Select the method to identify the secondary objects:Watershed - Image Select the input image:Membrane Number of pixels by which to expand the primary objects:45 Regularization factor:0.05 Discard secondary objects touching the border of the image?:No Discard the associated primary objects?:No Name the new primary objects:FilteredNuclei Fill holes in identified objects?:Yes Threshold setting version:12 Threshold strategy:Global Thresholding method:Otsu Threshold smoothing scale:0.0 Threshold correction factor:1.0 Lower and upper bounds on threshold:0.0,1.0 Manual threshold:0.0 Select the measurement to threshold with:None Two-class or three-class thresholding?:Two classes Log transform before thresholding?:No Assign pixels in the middle intensity class to the foreground or the background?:Foreground Size of adaptive window:50 Lower outlier fraction:0.05 Upper outlier fraction:0.05 Averaging method:Mean Variance method:Standard deviation # of deviations:2.0 Thresholding method:Otsu ConvertObjectsToImage:[module_num:5|svn_version:'Unknown'|variable_revision_number:1|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input objects:Cells Name the output image:CellImage Select the color format:uint16 Select the colormap:Default MeasureObjectIntensity:[module_num:6|svn_version:'Unknown'|variable_revision_number:4|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select images to measure:CAMK4, CD11c, CD16, CD20, CD27, CD3, CD34, CD4, CD45, CD45RA, CD45RO, CD68, CD8a, CollagenIV, DNA1, FoxP3, IFNA5, IgA, IgM, Ki67, PD1, PDL1, PanKeratin, SMA, VEGFc, Vimentin, eCadherin Select objects to measure:Cells MeasureObjectSizeShape:[module_num:7|svn_version:'Unknown'|variable_revision_number:3|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select object sets to measure:Cells Calculate the Zernike features?:No Calculate the advanced features?:No SaveImages:[module_num:8|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the type of image to save:Image Select the image to save:CellImage Select method for constructing file names:Single name Select image name for file prefix:None Enter single file name:\g-CellProfiler4-Cell_Mask Number of digits:4 Append a suffix to the image file name?:No Text to append to the image name: Saved file format:tiff Output file location:Default Output Folder| Image bit depth:16-bit integer Overwrite existing files without warning?:No When to save:Every cycle Record the file and path information to the saved image?:No Create subfolders in the output folder?:No Base image folder:Elsewhere...| How to save the series:T (Time) Save with lossless compression?:No ExportToSpreadsheet:[module_num:9|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the column delimiter:Comma (",") Add image metadata columns to your object data file?:Yes Add image file and folder names to your object data file?:No Select the measurements to export:Yes Calculate the per-image mean values for object measurements?:No Calculate the per-image median values for object measurements?:No Calculate the per-image standard deviation values for object measurements?:No Output file location:Default Output Folder| Create a GenePattern GCT file?:No Select source of sample row name:Metadata Select the image to use as the identifier:None Select the metadata to use as the identifier:None Export all measurement types?:No Press button to select measurements:Cells|Intensity_IntegratedIntensityEdge_Ki67,Cells|Intensity_IntegratedIntensityEdge_CD11c,Cells|Intensity_IntegratedIntensityEdge_CD68,Cells|Intensity_IntegratedIntensityEdge_CD16,Cells|Intensity_IntegratedIntensityEdge_CD4,Cells|Intensity_IntegratedIntensityEdge_PanKeratin,Cells|Intensity_IntegratedIntensityEdge_IgA,Cells|Intensity_IntegratedIntensityEdge_Vimentin,Cells|Intensity_IntegratedIntensityEdge_CD27,Cells|Intensity_IntegratedIntensityEdge_CD45RA,Cells|Intensity_IntegratedIntensityEdge_CD3,Cells|Intensity_IntegratedIntensityEdge_IgM,Cells|Intensity_IntegratedIntensityEdge_CD8a,Cells|Intensity_IntegratedIntensityEdge_CD20,Cells|Intensity_IntegratedIntensityEdge_CAMK4,Cells|Intensity_IntegratedIntensityEdge_VEGFc,Cells|Intensity_IntegratedIntensityEdge_IFNA5,Cells|Intensity_IntegratedIntensityEdge_CollagenIV,Cells|Intensity_IntegratedIntensityEdge_SMA,Cells|Intensity_IntegratedIntensityEdge_FoxP3,Cells|Intensity_IntegratedIntensityEdge_CD34,Cells|Intensity_IntegratedIntensityEdge_DNA1,Cells|Intensity_IntegratedIntensityEdge_PD1,Cells|Intensity_IntegratedIntensityEdge_CD45,Cells|Intensity_IntegratedIntensityEdge_CD45RO,Cells|Intensity_IntegratedIntensityEdge_eCadherin,Cells|Intensity_IntegratedIntensityEdge_PDL1,Cells|Intensity_IntegratedIntensity_DNA1,Cells|Intensity_IntegratedIntensity_CollagenIV,Cells|Intensity_IntegratedIntensity_Vimentin,Cells|Intensity_IntegratedIntensity_CD16,Cells|Intensity_IntegratedIntensity_CD27,Cells|Intensity_IntegratedIntensity_VEGFc,Cells|Intensity_IntegratedIntensity_CD8a,Cells|Intensity_IntegratedIntensity_Ki67,Cells|Intensity_IntegratedIntensity_SMA,Cells|Intensity_IntegratedIntensity_CD45RA,Cells|Intensity_IntegratedIntensity_CD11c,Cells|Intensity_IntegratedIntensity_CAMK4,Cells|Intensity_IntegratedIntensity_CD4,Cells|Intensity_IntegratedIntensity_eCadherin,Cells|Intensity_IntegratedIntensity_IFNA5,Cells|Intensity_IntegratedIntensity_CD45RO,Cells|Intensity_IntegratedIntensity_PD1,Cells|Intensity_IntegratedIntensity_FoxP3,Cells|Intensity_IntegratedIntensity_CD20,Cells|Intensity_IntegratedIntensity_CD45,Cells|Intensity_IntegratedIntensity_IgA,Cells|Intensity_IntegratedIntensity_PanKeratin,Cells|Intensity_IntegratedIntensity_CD3,Cells|Intensity_IntegratedIntensity_CD34,Cells|Intensity_IntegratedIntensity_IgM,Cells|Intensity_IntegratedIntensity_PDL1,Cells|Intensity_IntegratedIntensity_CD68,Cells|Intensity_LowerQuartileIntensity_CD8a,Cells|Intensity_LowerQuartileIntensity_CD11c,Cells|Intensity_LowerQuartileIntensity_PanKeratin,Cells|Intensity_LowerQuartileIntensity_CD68,Cells|Intensity_LowerQuartileIntensity_IgM,Cells|Intensity_LowerQuartileIntensity_SMA,Cells|Intensity_LowerQuartileIntensity_CD16,Cells|Intensity_LowerQuartileIntensity_CD27,Cells|Intensity_LowerQuartileIntensity_VEGFc,Cells|Intensity_LowerQuartileIntensity_CD4,Cells|Intensity_LowerQuartileIntensity_IgA,Cells|Intensity_LowerQuartileIntensity_FoxP3,Cells|Intensity_LowerQuartileIntensity_CD20,Cells|Intensity_LowerQuartileIntensity_eCadherin,Cells|Intensity_LowerQuartileIntensity_Vimentin,Cells|Intensity_LowerQuartileIntensity_Ki67,Cells|Intensity_LowerQuartileIntensity_PD1,Cells|Intensity_LowerQuartileIntensity_CD3,Cells|Intensity_LowerQuartileIntensity_CD45RO,Cells|Intensity_LowerQuartileIntensity_PDL1,Cells|Intensity_LowerQuartileIntensity_CAMK4,Cells|Intensity_LowerQuartileIntensity_DNA1,Cells|Intensity_LowerQuartileIntensity_CD34,Cells|Intensity_LowerQuartileIntensity_CD45,Cells|Intensity_LowerQuartileIntensity_CollagenIV,Cells|Intensity_LowerQuartileIntensity_IFNA5,Cells|Intensity_LowerQuartileIntensity_CD45RA,Cells|Intensity_MADIntensity_CD3,Cells|Intensity_MADIntensity_IFNA5,Cells|Intensity_MADIntensity_CD45RO,Cells|Intensity_MADIntensity_CD45,Cells|Intensity_MADIntensity_DNA1,Cells|Intensity_MADIntensity_SMA,Cells|Intensity_MADIntensity_CD20,Cells|Intensity_MADIntensity_IgM,Cells|Intensity_MADIntensity_CollagenIV,Cells|Intensity_MADIntensity_CD68,Cells|Intensity_MADIntensity_CD34,Cells|Intensity_MADIntensity_Ki67,Cells|Intensity_MADIntensity_PanKeratin,Cells|Intensity_MADIntensity_IgA,Cells|Intensity_MADIntensity_Vimentin,Cells|Intensity_MADIntensity_CD4,Cells|Intensity_MADIntensity_CD45RA,Cells|Intensity_MADIntensity_eCadherin,Cells|Intensity_MADIntensity_CAMK4,Cells|Intensity_MADIntensity_CD27,Cells|Intensity_MADIntensity_CD11c,Cells|Intensity_MADIntensity_CD8a,Cells|Intensity_MADIntensity_PDL1,Cells|Intensity_MADIntensity_CD16,Cells|Intensity_MADIntensity_PD1,Cells|Intensity_MADIntensity_VEGFc,Cells|Intensity_MADIntensity_FoxP3,Cells|Intensity_MinIntensity_CD45,Cells|Intensity_MinIntensity_PDL1,Cells|Intensity_MinIntensity_DNA1,Cells|Intensity_MinIntensity_CD4,Cells|Intensity_MinIntensity_FoxP3,Cells|Intensity_MinIntensity_SMA,Cells|Intensity_MinIntensity_PanKeratin,Cells|Intensity_MinIntensity_CD8a,Cells|Intensity_MinIntensity_CD20,Cells|Intensity_MinIntensity_CD34,Cells|Intensity_MinIntensity_VEGFc,Cells|Intensity_MinIntensity_IgM,Cells|Intensity_MinIntensity_CD45RO,Cells|Intensity_MinIntensity_CD16,Cells|Intensity_MinIntensity_eCadherin,Cells|Intensity_MinIntensity_IgA,Cells|Intensity_MinIntensity_PD1,Cells|Intensity_MinIntensity_Ki67,Cells|Intensity_MinIntensity_IFNA5,Cells|Intensity_MinIntensity_Vimentin,Cells|Intensity_MinIntensity_CD11c,Cells|Intensity_MinIntensity_CD45RA,Cells|Intensity_MinIntensity_CAMK4,Cells|Intensity_MinIntensity_CD3,Cells|Intensity_MinIntensity_CollagenIV,Cells|Intensity_MinIntensity_CD68,Cells|Intensity_MinIntensity_CD27,Cells|Intensity_MassDisplacement_CD45RO,Cells|Intensity_MassDisplacement_PanKeratin,Cells|Intensity_MassDisplacement_CD45,Cells|Intensity_MassDisplacement_PDL1,Cells|Intensity_MassDisplacement_CD3,Cells|Intensity_MassDisplacement_SMA,Cells|Intensity_MassDisplacement_CD16,Cells|Intensity_MassDisplacement_CD68,Cells|Intensity_MassDisplacement_VEGFc,Cells|Intensity_MassDisplacement_PD1,Cells|Intensity_MassDisplacement_CD11c,Cells|Intensity_MassDisplacement_IgM,Cells|Intensity_MassDisplacement_CD20,Cells|Intensity_MassDisplacement_IgA,Cells|Intensity_MassDisplacement_IFNA5,Cells|Intensity_MassDisplacement_eCadherin,Cells|Intensity_MassDisplacement_Ki67,Cells|Intensity_MassDisplacement_CAMK4,Cells|Intensity_MassDisplacement_FoxP3,Cells|Intensity_MassDisplacement_CD45RA,Cells|Intensity_MassDisplacement_Vimentin,Cells|Intensity_MassDisplacement_CD34,Cells|Intensity_MassDisplacement_CD27,Cells|Intensity_MassDisplacement_DNA1,Cells|Intensity_MassDisplacement_CollagenIV,Cells|Intensity_MassDisplacement_CD4,Cells|Intensity_MassDisplacement_CD8a,Cells|Intensity_MaxIntensity_CD4,Cells|Intensity_MaxIntensity_CollagenIV,Cells|Intensity_MaxIntensity_CD27,Cells|Intensity_MaxIntensity_CAMK4,Cells|Intensity_MaxIntensity_IgA,Cells|Intensity_MaxIntensity_PDL1,Cells|Intensity_MaxIntensity_SMA,Cells|Intensity_MaxIntensity_Vimentin,Cells|Intensity_MaxIntensity_FoxP3,Cells|Intensity_MaxIntensity_CD45,Cells|Intensity_MaxIntensity_VEGFc,Cells|Intensity_MaxIntensity_PD1,Cells|Intensity_MaxIntensity_CD34,Cells|Intensity_MaxIntensity_CD8a,Cells|Intensity_MaxIntensity_DNA1,Cells|Intensity_MaxIntensity_Ki67,Cells|Intensity_MaxIntensity_PanKeratin,Cells|Intensity_MaxIntensity_CD3,Cells|Intensity_MaxIntensity_CD20,Cells|Intensity_MaxIntensity_IgM,Cells|Intensity_MaxIntensity_CD11c,Cells|Intensity_MaxIntensity_CD45RO,Cells|Intensity_MaxIntensity_IFNA5,Cells|Intensity_MaxIntensity_eCadherin,Cells|Intensity_MaxIntensity_CD16,Cells|Intensity_MaxIntensity_CD45RA,Cells|Intensity_MaxIntensity_CD68,Cells|Intensity_UpperQuartileIntensity_CD45RO,Cells|Intensity_UpperQuartileIntensity_PanKeratin,Cells|Intensity_UpperQuartileIntensity_CD45RA,Cells|Intensity_UpperQuartileIntensity_FoxP3,Cells|Intensity_UpperQuartileIntensity_Vimentin,Cells|Intensity_UpperQuartileIntensity_CD20,Cells|Intensity_UpperQuartileIntensity_CD4,Cells|Intensity_UpperQuartileIntensity_DNA1,Cells|Intensity_UpperQuartileIntensity_CD27,Cells|Intensity_UpperQuartileIntensity_IFNA5,Cells|Intensity_UpperQuartileIntensity_VEGFc,Cells|Intensity_UpperQuartileIntensity_CD16,Cells|Intensity_UpperQuartileIntensity_eCadherin,Cells|Intensity_UpperQuartileIntensity_CAMK4,Cells|Intensity_UpperQuartileIntensity_SMA,Cells|Intensity_UpperQuartileIntensity_CD8a,Cells|Intensity_UpperQuartileIntensity_CollagenIV,Cells|Intensity_UpperQuartileIntensity_PDL1,Cells|Intensity_UpperQuartileIntensity_CD11c,Cells|Intensity_UpperQuartileIntensity_IgM,Cells|Intensity_UpperQuartileIntensity_Ki67,Cells|Intensity_UpperQuartileIntensity_CD3,Cells|Intensity_UpperQuartileIntensity_IgA,Cells|Intensity_UpperQuartileIntensity_CD68,Cells|Intensity_UpperQuartileIntensity_CD34,Cells|Intensity_UpperQuartileIntensity_CD45,Cells|Intensity_UpperQuartileIntensity_PD1,Cells|Intensity_MinIntensityEdge_Vimentin,Cells|Intensity_MinIntensityEdge_CD45,Cells|Intensity_MinIntensityEdge_CD68,Cells|Intensity_MinIntensityEdge_CD45RO,Cells|Intensity_MinIntensityEdge_CD8a,Cells|Intensity_MinIntensityEdge_CD20,Cells|Intensity_MinIntensityEdge_IgM,Cells|Intensity_MinIntensityEdge_IgA,Cells|Intensity_MinIntensityEdge_IFNA5,Cells|Intensity_MinIntensityEdge_CollagenIV,Cells|Intensity_MinIntensityEdge_CD3,Cells|Intensity_MinIntensityEdge_CD34,Cells|Intensity_MinIntensityEdge_PD1,Cells|Intensity_MinIntensityEdge_DNA1,Cells|Intensity_MinIntensityEdge_CD4,Cells|Intensity_MinIntensityEdge_CAMK4,Cells|Intensity_MinIntensityEdge_CD11c,Cells|Intensity_MinIntensityEdge_CD16,Cells|Intensity_MinIntensityEdge_eCadherin,Cells|Intensity_MinIntensityEdge_PanKeratin,Cells|Intensity_MinIntensityEdge_SMA,Cells|Intensity_MinIntensityEdge_FoxP3,Cells|Intensity_MinIntensityEdge_Ki67,Cells|Intensity_MinIntensityEdge_CD45RA,Cells|Intensity_MinIntensityEdge_VEGFc,Cells|Intensity_MinIntensityEdge_CD27,Cells|Intensity_MinIntensityEdge_PDL1,Cells|Intensity_StdIntensityEdge_PanKeratin,Cells|Intensity_StdIntensityEdge_CD16,Cells|Intensity_StdIntensityEdge_IFNA5,Cells|Intensity_StdIntensityEdge_PD1,Cells|Intensity_StdIntensityEdge_PDL1,Cells|Intensity_StdIntensityEdge_FoxP3,Cells|Intensity_StdIntensityEdge_IgA,Cells|Intensity_StdIntensityEdge_Ki67,Cells|Intensity_StdIntensityEdge_CD11c,Cells|Intensity_StdIntensityEdge_CD20,Cells|Intensity_StdIntensityEdge_CD8a,Cells|Intensity_StdIntensityEdge_CD4,Cells|Intensity_StdIntensityEdge_IgM,Cells|Intensity_StdIntensityEdge_eCadherin,Cells|Intensity_StdIntensityEdge_CD3,Cells|Intensity_StdIntensityEdge_CD68,Cells|Intensity_StdIntensityEdge_DNA1,Cells|Intensity_StdIntensityEdge_Vimentin,Cells|Intensity_StdIntensityEdge_VEGFc,Cells|Intensity_StdIntensityEdge_CD45,Cells|Intensity_StdIntensityEdge_CollagenIV,Cells|Intensity_StdIntensityEdge_SMA,Cells|Intensity_StdIntensityEdge_CD27,Cells|Intensity_StdIntensityEdge_CAMK4,Cells|Intensity_StdIntensityEdge_CD34,Cells|Intensity_StdIntensityEdge_CD45RO,Cells|Intensity_StdIntensityEdge_CD45RA,Cells|Intensity_MedianIntensity_PDL1,Cells|Intensity_MedianIntensity_PanKeratin,Cells|Intensity_MedianIntensity_CD45RA,Cells|Intensity_MedianIntensity_CD11c,Cells|Intensity_MedianIntensity_CD34,Cells|Intensity_MedianIntensity_DNA1,Cells|Intensity_MedianIntensity_eCadherin,Cells|Intensity_MedianIntensity_CD27,Cells|Intensity_MedianIntensity_VEGFc,Cells|Intensity_MedianIntensity_CD4,Cells|Intensity_MedianIntensity_CD68,Cells|Intensity_MedianIntensity_CD45RO,Cells|Intensity_MedianIntensity_Ki67,Cells|Intensity_MedianIntensity_SMA,Cells|Intensity_MedianIntensity_IgA,Cells|Intensity_MedianIntensity_CD16,Cells|Intensity_MedianIntensity_Vimentin,Cells|Intensity_MedianIntensity_CD8a,Cells|Intensity_MedianIntensity_CD3,Cells|Intensity_MedianIntensity_CD20,Cells|Intensity_MedianIntensity_IgM,Cells|Intensity_MedianIntensity_PD1,Cells|Intensity_MedianIntensity_IFNA5,Cells|Intensity_MedianIntensity_FoxP3,Cells|Intensity_MedianIntensity_CAMK4,Cells|Intensity_MedianIntensity_CollagenIV,Cells|Intensity_MedianIntensity_CD45,Cells|Intensity_StdIntensity_VEGFc,Cells|Intensity_StdIntensity_eCadherin,Cells|Intensity_StdIntensity_CAMK4,Cells|Intensity_StdIntensity_CD45RA,Cells|Intensity_StdIntensity_CD34,Cells|Intensity_StdIntensity_DNA1,Cells|Intensity_StdIntensity_FoxP3,Cells|Intensity_StdIntensity_IgA,Cells|Intensity_StdIntensity_Ki67,Cells|Intensity_StdIntensity_CD16,Cells|Intensity_StdIntensity_CD8a,Cells|Intensity_StdIntensity_CD27,Cells|Intensity_StdIntensity_CD68,Cells|Intensity_StdIntensity_CD3,Cells|Intensity_StdIntensity_PanKeratin,Cells|Intensity_StdIntensity_CD11c,Cells|Intensity_StdIntensity_SMA,Cells|Intensity_StdIntensity_CD4,Cells|Intensity_StdIntensity_CollagenIV,Cells|Intensity_StdIntensity_PDL1,Cells|Intensity_StdIntensity_CD20,Cells|Intensity_StdIntensity_Vimentin,Cells|Intensity_StdIntensity_IFNA5,Cells|Intensity_StdIntensity_CD45,Cells|Intensity_StdIntensity_IgM,Cells|Intensity_StdIntensity_CD45RO,Cells|Intensity_StdIntensity_PD1,Cells|Intensity_MeanIntensity_IgM,Cells|Intensity_MeanIntensity_CD68,Cells|Intensity_MeanIntensity_FoxP3,Cells|Intensity_MeanIntensity_eCadherin,Cells|Intensity_MeanIntensity_Ki67,Cells|Intensity_MeanIntensity_CD45RA,Cells|Intensity_MeanIntensity_DNA1,Cells|Intensity_MeanIntensity_VEGFc,Cells|Intensity_MeanIntensity_CD34,Cells|Intensity_MeanIntensity_SMA,Cells|Intensity_MeanIntensity_CD11c,Cells|Intensity_MeanIntensity_CollagenIV,Cells|Intensity_MeanIntensity_CD3,Cells|Intensity_MeanIntensity_PD1,Cells|Intensity_MeanIntensity_CD16,Cells|Intensity_MeanIntensity_CD45RO,Cells|Intensity_MeanIntensity_IgA,Cells|Intensity_MeanIntensity_Vimentin,Cells|Intensity_MeanIntensity_CD27,Cells|Intensity_MeanIntensity_CD8a,Cells|Intensity_MeanIntensity_CD4,Cells|Intensity_MeanIntensity_PanKeratin,Cells|Intensity_MeanIntensity_CD20,Cells|Intensity_MeanIntensity_PDL1,Cells|Intensity_MeanIntensity_CAMK4,Cells|Intensity_MeanIntensity_CD45,Cells|Intensity_MeanIntensity_IFNA5,Cells|Intensity_MeanIntensityEdge_FoxP3,Cells|Intensity_MeanIntensityEdge_CD3,Cells|Intensity_MeanIntensityEdge_CD16,Cells|Intensity_MeanIntensityEdge_PanKeratin,Cells|Intensity_MeanIntensityEdge_CollagenIV,Cells|Intensity_MeanIntensityEdge_DNA1,Cells|Intensity_MeanIntensityEdge_CD45RA,Cells|Intensity_MeanIntensityEdge_PD1,Cells|Intensity_MeanIntensityEdge_CAMK4,Cells|Intensity_MeanIntensityEdge_IFNA5,Cells|Intensity_MeanIntensityEdge_SMA,Cells|Intensity_MeanIntensityEdge_VEGFc,Cells|Intensity_MeanIntensityEdge_CD45,Cells|Intensity_MeanIntensityEdge_Ki67,Cells|Intensity_MeanIntensityEdge_IgM,Cells|Intensity_MeanIntensityEdge_Vimentin,Cells|Intensity_MeanIntensityEdge_CD27,Cells|Intensity_MeanIntensityEdge_CD8a,Cells|Intensity_MeanIntensityEdge_IgA,Cells|Intensity_MeanIntensityEdge_CD20,Cells|Intensity_MeanIntensityEdge_CD34,Cells|Intensity_MeanIntensityEdge_CD45RO,Cells|Intensity_MeanIntensityEdge_PDL1,Cells|Intensity_MeanIntensityEdge_eCadherin,Cells|Intensity_MeanIntensityEdge_CD4,Cells|Intensity_MeanIntensityEdge_CD11c,Cells|Intensity_MeanIntensityEdge_CD68,Cells|Intensity_MaxIntensityEdge_Vimentin,Cells|Intensity_MaxIntensityEdge_DNA1,Cells|Intensity_MaxIntensityEdge_CD34,Cells|Intensity_MaxIntensityEdge_CD11c,Cells|Intensity_MaxIntensityEdge_VEGFc,Cells|Intensity_MaxIntensityEdge_PD1,Cells|Intensity_MaxIntensityEdge_CD27,Cells|Intensity_MaxIntensityEdge_CD20,Cells|Intensity_MaxIntensityEdge_IgA,Cells|Intensity_MaxIntensityEdge_CD8a,Cells|Intensity_MaxIntensityEdge_CD45RO,Cells|Intensity_MaxIntensityEdge_CD16,Cells|Intensity_MaxIntensityEdge_Ki67,Cells|Intensity_MaxIntensityEdge_eCadherin,Cells|Intensity_MaxIntensityEdge_CD45RA,Cells|Intensity_MaxIntensityEdge_CD68,Cells|Intensity_MaxIntensityEdge_IgM,Cells|Intensity_MaxIntensityEdge_CD3,Cells|Intensity_MaxIntensityEdge_CD4,Cells|Intensity_MaxIntensityEdge_CD45,Cells|Intensity_MaxIntensityEdge_CollagenIV,Cells|Intensity_MaxIntensityEdge_PDL1,Cells|Intensity_MaxIntensityEdge_FoxP3,Cells|Intensity_MaxIntensityEdge_SMA,Cells|Intensity_MaxIntensityEdge_IFNA5,Cells|Intensity_MaxIntensityEdge_CAMK4,Cells|Intensity_MaxIntensityEdge_PanKeratin,Cells|Location_Center_Y,Cells|Location_Center_Z,Cells|Location_Center_X,Cells|AreaShape_BoundingBoxMinimum_Y,Cells|AreaShape_BoundingBoxMinimum_X,Cells|AreaShape_Center_X,Cells|AreaShape_Center_Y,Cells|AreaShape_Eccentricity,Cells|AreaShape_MinorAxisLength,Cells|AreaShape_MedianRadius,Cells|AreaShape_MaxFeretDiameter,Cells|AreaShape_Extent,Cells|AreaShape_MajorAxisLength,Cells|AreaShape_Compactness,Cells|AreaShape_BoundingBoxArea,Cells|AreaShape_EquivalentDiameter,Cells|AreaShape_MeanRadius,Cells|AreaShape_EulerNumber,Cells|AreaShape_BoundingBoxMaximum_X,Cells|AreaShape_BoundingBoxMaximum_Y,Cells|AreaShape_FormFactor,Cells|AreaShape_Perimeter,Cells|AreaShape_Orientation,Cells|AreaShape_MinFeretDiameter,Cells|AreaShape_MaximumRadius,Cells|AreaShape_Solidity,Cells|AreaShape_Area Representation of Nan/Inf:NaN Add a prefix to file names?:No Filename prefix:MyExpt_ Overwrite existing files without warning?:Yes Data to export:Cells Combine these object measurements with those of the previous object?:No File name:\g-CellProfiler4-Cells.csv Use the object name for the file name?:No