eco.owl
type: Ontology
version IRI: eco.owl
comment: For more details, see https://github.com/evidenceontology/evidenceontology
auto-generated-by: OBO-Edit 2.3.1
default-namespace: eco
date: 03:28:2017 15:22
saved-by: rctauber
has_obo_format_version: 1.2

definition
type: AnnotationProperty

go groupings
type: AnnotationProperty
comment: Grouping classes used by GO
sub property of: subset_property

valid with biological process
type: AnnotationProperty
comment: GO biological process terms should be used in the GO with/from field
sub property of: subset_property

valid with cellular component
type: AnnotationProperty
comment: GO cellular component terms should be used in the GO with/from field
sub property of: subset_property

valid with chemical entity
type: AnnotationProperty
comment: Chemical entity IDs should be used in the GO with/from field
sub property of: subset_property

valid with gene
type: AnnotationProperty
comment: Gene IDs should be used in the GO with/from field
sub property of: subset_property

valid with molecular function
type: AnnotationProperty
comment: GO molecular function terms should be used in the GO with/from field
sub property of: subset_property

valid with protein
type: AnnotationProperty
comment: Protein IDs should be used in the GO with/from field
sub property of: subset_property

valid with protein complex
type: AnnotationProperty
comment: Protein complex IDs should be used in the GO with/from field
sub property of: subset_property

valid with transcript
type: AnnotationProperty
comment: Transcript IDs should be used in the GO with/from field
sub property of: subset_property

subset_property
type: AnnotationProperty

has_alternative_id
type: AnnotationProperty

has_broad_synonym
type: AnnotationProperty

database_cross_reference
type: AnnotationProperty

has_exact_synonym
type: AnnotationProperty

has_narrow_synonym
type: AnnotationProperty

has_obo_format_version
type: AnnotationProperty

has_obo_namespace
type: AnnotationProperty

has_related_synonym
type: AnnotationProperty

in_subset
type: AnnotationProperty

shorthand
type: AnnotationProperty

used_in
type: ObjectProperty
inverse of: uses
domain: evidence
range: assertion method
definition: A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence.
created by: mchibucos
creation date: 2010-12-09T05:00:20Z
database_cross_reference: ECO:9000000
has_obo_namespace: eco
id: used_in
shorthand: used_in
comment: In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI.

Axiom
annotated source: used_in
annotated property: definition
annotated target: A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence.
database_cross_reference: GOC:cjm

uses
type: ObjectProperty
database_cross_reference: ECO:9000001
has_obo_namespace: eco
id: uses
shorthand: uses

evidence
type: Class
disjoint with: assertion method
definition: A type of information that is used to support an assertion.
has_obo_namespace: eco
has_related_synonym: evidence code
has_related_synonym: evidence_code
id: ECO:0000000

Axiom
annotated source: evidence
annotated property: definition
annotated target: A type of information that is used to support an assertion.
database_cross_reference: ECO:MCC

inference from background scientific knowledge
type: Class
subclass of: curator inference
definition: A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator.
has_obo_namespace: eco
id: ECO:0000001

Axiom
annotated source: inference from background scientific knowledge
annotated property: definition
annotated target: A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go

direct assay evidence
type: Class
subclass of: experimental evidence
definition: Experimental evidence that is based on direct measurement of some aspect of a biological feature.
has_obo_namespace: eco
id: ECO:0000002

Axiom
annotated source: direct assay evidence
annotated property: definition
annotated target: Experimental evidence that is based on direct measurement of some aspect of a biological feature.
database_cross_reference: ECO:MCC

reconstitution assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state.
has_obo_namespace: eco
id: ECO:0000003

Axiom
annotated source: reconstitution assay evidence
annotated property: definition
annotated target: A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state.
database_cross_reference: ECO:KAV
database_cross_reference: PMID:26029343

cell fractionation evidence
type: Class
subclass of: fractionation evidence
definition: A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient.
has_obo_namespace: eco
has_related_synonym: IDA: cell fractionation
id: ECO:0000004
comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology.

Axiom
annotated source: cell fractionation evidence
annotated property: definition
annotated target: A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient.
database_cross_reference: ECO:KIM
database_cross_reference: TAIR:TED

enzyme assay evidence
type: Class
subclass of: protein assay evidence
definition: Used when an annotation is made based on assays that determine the catalytic activity of enzymes.
database_cross_reference: PSI-MI:MI:0415
has_obo_namespace: eco
has_related_synonym: IDA: enzyme assays
id: ECO:0000005

Axiom
annotated source: enzyme assay evidence
annotated property: definition
annotated target: Used when an annotation is made based on assays that determine the catalytic activity of enzymes.
database_cross_reference: TAIR:TED

enzymatic study
type: Axiom
annotated source: enzyme assay evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0415

experimental evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on the results of a laboratory assay.
has_obo_namespace: eco
id: ECO:0000006
in_subset: go groupings

Axiom
annotated source: experimental evidence
annotated property: definition
annotated target: An evidence type that is based on the results of a laboratory assay.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:ecd

immunofluorescence evidence
type: Class
subclass of: protein detection assay evidence
definition: A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell.
has_obo_namespace: eco
has_related_synonym: IDA: immunofluorescence
id: ECO:0000007

Axiom
annotated source: immunofluorescence evidence
annotated property: definition
annotated target: A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

expression pattern evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence that is based on characterization of gene expression.
has_obo_namespace: eco
id: ECO:0000008
comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process.

Axiom
annotated source: expression pattern evidence
annotated property: definition
annotated target: A type of experimental evidence that is based on characterization of gene expression.
database_cross_reference: ECO:MCC
database_cross_reference: GO:IEP

transcript expression evidence
type: Class
subclass of: expression pattern evidence
has_obo_namespace: eco
id: ECO:0000009

protein expression evidence
type: Class
subclass of: expression pattern evidence
subclass of: protein assay evidence
has_obo_namespace: eco
id: ECO:0000010

genetic interaction evidence
type: Class
subclass of: experimental phenotypic evidence
database_cross_reference: TAIR:TED:0000006
has_obo_namespace: eco
id: ECO:0000011

functional complementation evidence
type: Class
subclass of: genetic interaction evidence
definition: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene.
has_obo_namespace: eco
has_related_synonym: IGI: functional complementation
id: ECO:0000012

Axiom
annotated source: functional complementation evidence
annotated property: definition
annotated target: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene.
database_cross_reference: TAIR:TED

transgenic rescue experiment evidence
type: Class
subclass of: functional complementation evidence
has_obo_namespace: eco
id: ECO:0000013

mutant phenotype evidence
type: Class
subclass of: experimental phenotypic evidence
definition: The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible.
has_obo_namespace: eco
id: ECO:0000015

Axiom
annotated source: mutant phenotype evidence
annotated property: definition
annotated target: The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible.
database_cross_reference: GO:IMP

loss-of-function mutant phenotype evidence
type: Class
subclass of: mutant phenotype evidence
has_obo_namespace: eco
id: ECO:0000016

ectopic expression evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question.
has_obo_namespace: eco
has_related_synonym: IMP: analysis of overexpression/ectopic expression phenotype
id: ECO:0000017

Axiom
annotated source: ectopic expression evidence
annotated property: definition
annotated target: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question.
database_cross_reference: TAIR:TED

anti-sense experiment evidence
type: Class
subclass of: mutant phenotype evidence
definition: Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background.
has_obo_namespace: eco
has_related_synonym: IMP: anti-sense experiments
id: ECO:0000018

Axiom
annotated source: anti-sense experiment evidence
annotated property: definition
annotated target: Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background.
database_cross_reference: TAIR:TED

RNAi evidence
type: Class
subclass of: mutant phenotype evidence
definition: A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed.
has_obo_namespace: eco
has_related_synonym: IMP: RNAi experiment
id: ECO:0000019

Axiom
annotated source: RNAi evidence
annotated property: definition
annotated target: A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed.
comment: ECO:MCC

specific protein inhibition evidence
type: Class
subclass of: protein assay evidence
definition: A type of protein assay evidence based on the inhibition of the molecular function of a protein.
has_obo_namespace: eco
id: ECO:0000020

Axiom
annotated source: specific protein inhibition evidence
annotated property: definition
annotated target: A type of protein assay evidence based on the inhibition of the molecular function of a protein.
database_cross_reference: ECO:MCC

physical interaction evidence
type: Class
subclass of: experimental evidence
definition: Experimental evidence that is based on characterization of an interaction between a gene product and another molecule.
database_cross_reference: PSI-MI:MI:0045
has_obo_namespace: eco
id: ECO:0000021
comment: Molecules interacted with might include protein, nucleic acid, ion, or complex.

Axiom
annotated source: physical interaction evidence
annotated property: definition
annotated target: Experimental evidence that is based on characterization of an interaction between a gene product and another molecule.
database_cross_reference: ECO:MCC

experimental interaction detection
type: Axiom
annotated source: physical interaction evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0045

co-purification evidence
type: Class
subclass of: physical interaction evidence
definition: A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex.
database_cross_reference: PSI-MI:MI:0025
has_obo_namespace: eco
has_related_synonym: IPI: co-purification
id: ECO:0000022

Axiom
annotated source: co-purification evidence
annotated property: definition
annotated target: A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex.
database_cross_reference: TAIR:TED

copurification
type: Axiom
annotated source: co-purification evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0025

affinity evidence
type: Class
subclass of: physical interaction evidence
definition: A type of physical interaction evidence that depends on the strength of the interaction between two entities.
database_cross_reference: PSI-MI:MI:0400
has_narrow_synonym: ligand binding evidence
has_obo_namespace: eco
id: ECO:0000023

Axiom
annotated source: affinity evidence
annotated property: definition
annotated target: A type of physical interaction evidence that depends on the strength of the interaction between two entities.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0400

affinity technology
type: Axiom
annotated source: affinity evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0400

protein binding evidence
type: Class
subclass of: affinity evidence
subclass of: protein assay evidence
has_obo_namespace: eco
id: ECO:0000024

hybrid interaction evidence
type: Class
subclass of: affinity evidence
definition: A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.
database_cross_reference: PSI-MI:MI:0090
has_obo_namespace: eco
id: ECO:0000025
comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.

Axiom
annotated source: hybrid interaction evidence
annotated property: definition
annotated target: A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0090

protein complementation assay
type: Axiom
annotated source: hybrid interaction evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0090

nucleic acid hybridization evidence
type: Class
subclass of: experimental genomic evidence
has_obo_namespace: eco
id: ECO:0000026

structural similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes.
has_obo_namespace: eco
id: ECO:0000027
comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field.

Axiom
annotated source: structural similarity evidence
annotated property: definition
annotated target: A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

motif similarity evidence
type: Class
subclass of: match to sequence model evidence
definition: Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence.
has_obo_namespace: eco
has_related_synonym: ISS: recognized domains
id: ECO:0000028

Axiom
annotated source: motif similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence.
database_cross_reference: TAIR:TED

match to InterPro member signature evidence
type: Class
subclass of: match to sequence model evidence
has_obo_namespace: eco
id: ECO:0000029

BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence
subclass of: pairwise sequence alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of BLAST evidence that is used in a manual assertion.
has_obo_namespace: eco
has_related_synonym: curated BLAST analysis
id: ECO:0000030

Axiom
annotated source: BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: pairwise sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: BLAST evidence used in manual assertion
annotated property: definition
annotated target: A type of BLAST evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence used in manual assertion
subclass of: protein BLAST evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Protein BLAST evidence that is used in a manual assertion.
database_cross_reference: GO_REF:0000012
database_cross_reference: GO_REF:0000027
has_obo_namespace: eco
has_related_synonym: curated protein BLAST analysis
id: ECO:0000031

Axiom
annotated source: protein BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: BLAST evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein BLAST evidence used in manual assertion
annotated property: definition
annotated target: Protein BLAST evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Pairwise alignment (TIGR)
type: Axiom
annotated source: protein BLAST evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000012

BLAST search criteria for ISS assignment in PAMGO_GAT
type: Axiom
annotated source: protein BLAST evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000027

nucleotide BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nucleotide BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence used in manual assertion
subclass of: nucleotide BLAST evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Nucleotide BLAST evidence that is used in a manual assertion.
has_obo_namespace: eco
has_related_synonym: curated nucleic acid BLAST analysis
id: ECO:0000032

Axiom
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: BLAST evidence used in manual assertion
is inferred: true

Axiom
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: definition
annotated target: Nucleotide BLAST evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

traceable author statement
type: Class
subclass of: author statement
disjoint with: non-traceable author statement
definition: A type of author statement that is based on a cited reference.
has_obo_namespace: eco
id: ECO:0000033
comment: The traceable author statement (TAS) evidence code covers author statements that are attributed to a cited source. Typically this type of information comes from review articles. Material from the introductions and discussion sections of non-review papers may also be suitable if another reference is cited as the source of experimental work or analysis. When annotating with this code the curator should use caution and be aware that authors often cite papers dealing with experiments that were performed in organisms different from the one being discussed in the paper at hand. Thus a problem with the TAS code is that it may turn out from following up the references in the paper that no experiments were performed on the gene in the organism actually being characterized in the primary paper. For this reason we recommend (when time and resources allow) that curators track down the cited paper and annotate directly from the experimental paper using the appropriate experimental evidence code. When this is not possible and it is necessary to annotate from reviews, the TAS code is the appropriate code to use for statements that are associated with a cited reference. Once an annotation has been made to a given term using an experimental evidence code, we recommend removing any annotations made to the same term using the TAS evidence code.

Axiom
annotated source: traceable author statement
annotated property: definition
annotated target: A type of author statement that is based on a cited reference.
database_cross_reference: ECO:MCC
database_cross_reference: GO:TAS

non-traceable author statement
type: Class
subclass of: author statement
definition: An author statement that is not associated with results presented or a cited reference.
has_obo_namespace: eco
id: ECO:0000034
comment: The non-traceable author statement evidence code should be used in all cases where the author makes a statement that a curator wants to capture but for which there are neither results presented nor a specific reference cited in the source used to make the annotation. The source of the information may be peer reviewed papers, textbooks, database records or vouchered specimens.

Axiom
annotated source: non-traceable author statement
annotated property: definition
annotated target: An author statement that is not associated with results presented or a cited reference.
database_cross_reference: ECO:MCC

no biological data found
type: Class
subclass of: curator inference
definition: A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources.
has_obo_namespace: eco
id: ECO:0000035
comment: An assertion of "no biological data found" carries the assumption that a more-or-less exhaustive search has been conducted.

Axiom
annotated source: no biological data found
annotated property: definition
annotated target: A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources.
database_cross_reference: ECO:MCC
database_cross_reference: GO:ND

not_recorded
type: Class
definition: An evidence that reflects an annotation was made before curators began tracking evidence types.
has_exact_synonym: NR
has_exact_synonym: not recorded
has_obo_namespace: eco
id: ECO:0000037
comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations.
deprecated: true

Axiom
annotated source: not_recorded
annotated property: definition
annotated target: An evidence that reflects an annotation was made before curators began tracking evidence types.
database_cross_reference: ECO:MCC
database_cross_reference: GO:NR

Axiom
annotated source: not_recorded
annotated property: has_exact_synonym
annotated target: NR
database_cross_reference: GO:NR

Axiom
annotated source: not_recorded
annotated property: has_exact_synonym
annotated target: not recorded
database_cross_reference: GO:NR

transient rescue experiment evidence
type: Class
subclass of: functional complementation evidence
has_obo_namespace: eco
id: ECO:0000038

protein assay evidence
type: Class
subclass of: direct assay evidence
has_obo_namespace: eco
id: ECO:0000039

immunological assay evidence
type: Class
subclass of: affinity evidence
has_obo_namespace: eco
id: ECO:0000040

similarity evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on comparing likeness of distinct biological entities.
has_exact_synonym: IS
has_obo_namespace: eco
has_related_synonym: inferred from similarity
id: ECO:0000041

Axiom
annotated source: similarity evidence
annotated property: definition
annotated target: An evidence type that is based on comparing likeness of distinct biological entities.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IS

gain-of-function mutant phenotype evidence
type: Class
subclass of: mutant phenotype evidence
has_obo_namespace: eco
id: ECO:0000042

sequence similarity evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity based on biomolecular sequence.
has_obo_namespace: eco
id: ECO:0000044
comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes.

Axiom
annotated source: sequence similarity evidence
annotated property: definition
annotated target: A type of similarity based on biomolecular sequence.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

spatial pattern of protein expression evidence
type: Class
subclass of: protein expression evidence
has_obo_namespace: eco
id: ECO:0000045

protein expression level evidence
type: Class
subclass of: protein expression evidence
has_obo_namespace: eco
id: ECO:0000046

spatial pattern of transcript expression evidence
type: Class
subclass of: transcript expression evidence
has_obo_namespace: eco
id: ECO:0000047

transcript expression level evidence
type: Class
subclass of: transcript expression evidence
has_obo_namespace: eco
id: ECO:0000048

reporter gene assay evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response.
has_obo_namespace: eco
has_related_synonym: IEP: expression of a reporter gene
id: ECO:0000049

Axiom
annotated source: reporter gene assay evidence
annotated property: definition
annotated target: Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response.
database_cross_reference: TAIR:TED

voucher specimen analysis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype.
has_exact_synonym: IVS
has_obo_namespace: eco
id: ECO:0000050

Axiom
annotated source: voucher specimen analysis evidence
annotated property: definition
annotated target: Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype.
database_cross_reference: PhenoScape:DOA

genetic similarity evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity based on genotype without respect to expression.
has_exact_synonym: IGTS
has_obo_namespace: eco
has_related_synonym: inferred from genetic similarity
id: ECO:0000051
comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology.

Axiom
annotated source: genetic similarity evidence
annotated property: definition
annotated target: A type of similarity based on genotype without respect to expression.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IGTS

suppressor/enhancer interaction evidence
type: Class
subclass of: genetic interaction evidence
database_cross_reference: TAIR:TED:0000063
has_obo_namespace: eco
has_related_synonym: IGI: 'traditional' genetic interactions (e.g. suppressors, synthetic lethals)
id: ECO:0000052

computational combinatorial evidence
type: Class
subclass of: combinatorial evidence
definition: A type of combinatorial analysis where data are combined and evaluated by an algorithm.
database_cross_reference: TAIR:TED:0000004
has_obo_namespace: eco
id: ECO:0000053
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.

Axiom
annotated source: computational combinatorial evidence
annotated property: definition
annotated target: A type of combinatorial analysis where data are combined and evaluated by an algorithm.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go

double mutant phenotype evidence
type: Class
subclass of: genetic interaction evidence
definition: Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus.
has_obo_namespace: eco
has_related_synonym: IGI: double mutant analysis
id: ECO:0000054

Axiom
annotated source: double mutant phenotype evidence
annotated property: definition
annotated target: Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus.
database_cross_reference: TAIT:TED

array experiment evidence
type: Class
subclass of: expression pattern evidence
has_obo_namespace: eco
id: ECO:0000055

epistatic interaction evidence
type: Class
subclass of: genetic interaction evidence
definition: Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus.
has_obo_namespace: eco
has_related_synonym: IGI: epistatic interactions
id: ECO:0000056

Axiom
annotated source: epistatic interaction evidence
annotated property: definition
annotated target: Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus.
database_cross_reference: TAIR:TED

phenotypic similarity evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity based on the expression of a genotype in an environment.
has_exact_synonym: IPTS
has_exact_synonym: phenotype similarity evidence
has_obo_namespace: eco
has_related_synonym: inferred from phenotypic similarity
id: ECO:0000057
comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms.

Axiom
annotated source: phenotypic similarity evidence
annotated property: definition
annotated target: A type of similarity based on the expression of a genotype in an environment.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IPTS

expression microarray evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000058

experimental phenotypic evidence
type: Class
subclass of: experimental evidence
definition: Experimental evidence that is based on the expression of a genotype in an environment.
has_alternative_id: ECO:0000014
has_obo_namespace: eco
has_related_synonym: inferred from phenotype
id: ECO:0000059

Axiom
annotated source: experimental phenotypic evidence
annotated property: definition
annotated target: Experimental evidence that is based on the expression of a genotype in an environment.
database_cross_reference: ECO:MCC

positional similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: Used when an annotation is made based on the similarity of the location and or arrangement of structures.
has_exact_synonym: IPS
has_obo_namespace: eco
id: ECO:0000060

Axiom
annotated source: positional similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the similarity of the location and or arrangement of structures.
database_cross_reference: PhenoScape:IPS

quantitative trait analysis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus.
has_obo_namespace: eco
has_related_synonym: IGI: quantitative trait analysis
id: ECO:0000061

Axiom
annotated source: quantitative trait analysis evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus.
database_cross_reference: TAIR:tn

genomic microarray evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000062

compositional similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: Used when an annotation is made based on the similarity of the histological makeup of structures.
has_exact_synonym: ICS
has_obo_namespace: eco
id: ECO:0000063

Axiom
annotated source: compositional similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the similarity of the histological makeup of structures.
database_cross_reference: PhenoScape:ICS

functional complementation in heterologous system evidence
type: Class
subclass of: functional complementation evidence
definition: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene.
has_obo_namespace: eco
has_related_synonym: IGI: functional complementation in heterologous system
id: ECO:0000064

Axiom
annotated source: functional complementation in heterologous system evidence
annotated property: definition
annotated target: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene.
database_cross_reference: TAIR:TED

CpG island microarray evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000065

yeast one-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
definition: A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors.
database_cross_reference: PSI-MI:MI:0432
has_obo_namespace: eco
has_related_synonym: IPI: yeast one-hybrid assay
id: ECO:0000066
comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED).

Axiom
annotated source: yeast one-hybrid evidence
annotated property: definition
annotated target: A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0432
database_cross_reference: TAIR:TED

one hybrid
type: Axiom
annotated source: yeast one-hybrid evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0432

developmental similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures.
has_exact_synonym: IDS
has_obo_namespace: eco
id: ECO:0000067

Axiom
annotated source: developmental similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures.
database_cross_reference: PhenoScape:IDS

yeast 2-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
definition: Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed.
has_obo_namespace: eco
has_related_synonym: IPI: yeast two-hybrid assay
id: ECO:0000068

Axiom
annotated source: yeast 2-hybrid evidence
annotated property: definition
annotated target: Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed.
database_cross_reference: TAIR:TED

differential methylation hybridization evidence
type: Class
subclass of: nucleic acid hybridization evidence
has_obo_namespace: eco
id: ECO:0000069

co-immunoprecipitation evidence
type: Class
subclass of: physical interaction evidence
subclass of: immunoprecipitation evidence
definition: A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification.
database_cross_reference: PSI-MI:MI:0019
has_obo_namespace: eco
has_related_synonym: IPI: co-immunoprecipitation
id: ECO:0000070
comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column.

Axiom
annotated source: co-immunoprecipitation evidence
annotated property: definition
annotated target: A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification.
database_cross_reference: ECO:MCC

coimmunoprecipitation
type: Axiom
annotated source: co-immunoprecipitation evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0019

morphological similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures.
has_exact_synonym: IMS
has_obo_namespace: eco
id: ECO:0000071

Axiom
annotated source: morphological similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures.
database_cross_reference: PhenoScape:IMS

Sos-recruitment assay evidence
type: Class
definition: A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout.
has_obo_namespace: eco
has_related_synonym: IPI: Sos-recruitment assay
id: ECO:0000072
deprecated: true

Axiom
annotated source: Sos-recruitment assay evidence
annotated property: definition
annotated target: A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout.
database_cross_reference: TAIR:TED

experimental genomic evidence
type: Class
subclass of: experimental evidence
definition: Experimental evidence that characterizes an attribute of the genome underlying a gene product.
has_exact_synonym: inferred from genomic analysis
has_obo_namespace: eco
id: ECO:0000073

Axiom
annotated source: experimental genomic evidence
annotated property: definition
annotated target: Experimental evidence that characterizes an attribute of the genome underlying a gene product.
database_cross_reference: ECO:MCC

split-ubiquitin assay evidence
type: Class
subclass of: physical interaction evidence
definition: Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs.
database_cross_reference: PSI-MI:MI:0112
has_obo_namespace: eco
has_related_synonym: IPI: split-ubiquitin assay
id: ECO:0000074

Axiom
annotated source: split-ubiquitin assay evidence
annotated property: definition
annotated target: Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs.
database_cross_reference: TAIR:TED

ubiquitin reconstruction
type: Axiom
annotated source: split-ubiquitin assay evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0112

gene expression similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: Used when an annotation is made based on the similarity of expression of genes in structures.
has_exact_synonym: IGES
has_obo_namespace: eco
id: ECO:0000075

Axiom
annotated source: gene expression similarity evidence
annotated property: definition
annotated target: Used when an annotation is made based on the similarity of expression of genes in structures.
database_cross_reference: PhenoScape:IGES

far-Western blotting evidence
type: Class
subclass of: physical interaction evidence
definition: Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column.
database_cross_reference: PSI-MI:MI:0047
has_obo_namespace: eco
has_related_synonym: IPI: far-Western analysis
id: ECO:0000076

Axiom
annotated source: far-Western blotting evidence
annotated property: definition
annotated target: Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column.
database_cross_reference: TAIR:TED

methylation-specific polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
has_exact_synonym: methylation-specific PCR evidence
has_obo_namespace: eco
id: ECO:0000077

southern hybridization evidence
type: Class
subclass of: DNA detection assay evidence
definition: Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question.
has_exact_synonym: Southern blot
has_obo_namespace: eco
has_related_synonym: ISS: Southern blotting
id: ECO:0000078

Axiom
annotated source: southern hybridization evidence
annotated property: definition
annotated target: Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question.
database_cross_reference: TAIR:TED

affinity chromatography evidence
type: Class
subclass of: affinity evidence
definition: A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound.
database_cross_reference: PSI-MI:MI:0004
has_obo_namespace: eco
has_related_synonym: IDA: affinity chromatography
id: ECO:0000079
comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand.

Axiom
annotated source: affinity chromatography evidence
annotated property: definition
annotated target: A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

affinity chromatography technology
type: Axiom
annotated source: affinity chromatography evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0004

phylogenetic evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity that indicates common ancestry.
has_exact_synonym: IP
has_obo_namespace: eco
id: ECO:0000080

Axiom
annotated source: phylogenetic evidence
annotated property: definition
annotated target: A type of similarity that indicates common ancestry.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IP

Axiom
annotated source: phylogenetic evidence
annotated property: has_exact_synonym
annotated target: IP
database_cross_reference: PhenoScape:IP

targeting sequence prediction evidence
type: Class
subclass of: motif similarity evidence
definition: Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence.
has_obo_namespace: eco
has_related_synonym: ISS: targeting sequence prediction
id: ECO:0000081

Axiom
annotated source: targeting sequence prediction evidence
annotated property: definition
annotated target: Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence.
database_cross_reference: TAIR:TED

polymerase chain reaction evidence
type: Class
subclass of: experimental genomic evidence
has_exact_synonym: PCR evidence
has_obo_namespace: eco
id: ECO:0000082

transmembrane domain prediction evidence
type: Class
subclass of: motif similarity evidence
definition: Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence.
has_obo_namespace: eco
has_related_synonym: ISS: transmembrane domain prediction
id: ECO:0000083

Axiom
annotated source: transmembrane domain prediction evidence
annotated property: definition
annotated target: Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence.
database_cross_reference: TAIR:TED

gene neighbors evidence
type: Class
subclass of: genomic context evidence
definition: Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes.
created by: mgiglio
creation date: 2009-03-20T11:55:18Z
database_cross_reference: GO_REF:0000025
has_exact_synonym: inferred from genome cluster
has_obo_namespace: eco
has_related_synonym: ICL
id: ECO:0000084
comment: Genomic cluster analyses include synteny and operon structure.

Axiom
annotated source: gene neighbors evidence
annotated property: definition
annotated target: Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:mg

Operon structure as IGC evidence
type: Axiom
annotated source: gene neighbors evidence
annotated property: database_cross_reference
annotated target: GO_REF:0000025

immunoprecipitation evidence
type: Class
subclass of: protein assay evidence
definition: A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation.
has_obo_namespace: eco
has_related_synonym: IDA: immunoprecipitation
id: ECO:0000085
comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding.

Axiom
annotated source: immunoprecipitation evidence
annotated property: definition
annotated target: A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: TAIR:TED

intermethylated site amplification evidence
type: Class
subclass of: experimental genomic evidence
has_obo_namespace: eco
id: ECO:0000086

immunolocalization evidence
type: Class
subclass of: microscopy evidence
subclass of: immunodetection assay evidence
definition: A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues.
has_obo_namespace: eco
has_related_synonym: IDA: immunolocalization
id: ECO:0000087

Axiom
annotated source: immunolocalization evidence
annotated property: definition
annotated target: A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SN

biological system reconstruction evidence
type: Class
subclass of: evidence
definition: A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system.
created by: mgiglio
creation date: 2009-03-20T12:00:17Z
has_obo_namespace: eco
has_related_synonym: ISR
has_related_synonym: inferred from system reconstruction
id: ECO:0000088
comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak.

Axiom
annotated source: biological system reconstruction evidence
annotated property: definition
annotated target: A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:mg

restriction landmark genomic scanning evidence
type: Class
subclass of: DNA detection assay evidence
has_alternative_id: ECO:0001125
has_exact_synonym: RLGS evidence
has_exact_synonym: restriction landmark genome scanning evidence
has_obo_namespace: eco
id: ECO:0000089

immunogold labelling evidence
type: Class
subclass of: immunolocalization evidence
definition: A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy.
has_obo_namespace: eco
has_related_synonym: IDA: immunogold labelling
id: ECO:0000090

Axiom
annotated source: immunogold labelling evidence
annotated property: definition
annotated target: A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy.
database_cross_reference: ECO:MCC

epitope-tagged protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
definition: Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies.
has_obo_namespace: eco
has_related_synonym: IDA: immunolocalization of epitope-tagged protein
id: ECO:0000092

Axiom
annotated source: epitope-tagged protein immunolocalization evidence
annotated property: definition
annotated target: Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies.
database_cross_reference: TAIR:TED

oligonucleotide microarray evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000093

biological assay evidence
type: Class
subclass of: direct assay evidence
definition: Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition.
has_obo_namespace: eco
has_related_synonym: IDA: bioassay
id: ECO:0000094

Axiom
annotated source: biological assay evidence
annotated property: definition
annotated target: Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

cell growth assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect.
created by: mchibucos
creation date: 2014-10-15T00:58:50Z
has_obo_namespace: eco
has_related_synonym: growth curve analysis
id: ECO:0000095
comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter.

Axiom
annotated source: cell growth assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect.
database_cross_reference: ECO:SW
database_cross_reference: PomBase:MAH

electrophoretic mobility shift assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions.
database_cross_reference: PSI-MI:MI:0413
has_exact_synonym: EMSA: electrophoretic mobility shift assay
has_obo_namespace: eco
has_related_synonym: Gel retardation assay
has_related_synonym: IDA: electrophoretic mobility shift assay
id: ECO:0000096
comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid.

Axiom
annotated source: electrophoretic mobility shift assay evidence
annotated property: definition
annotated target: A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions.
database_cross_reference: ECO:KIM
database_cross_reference: ECO:SW
database_cross_reference: PMID:17703195
database_cross_reference: TAIR:TED

electrophoretic mobility shift assay
type: Axiom
annotated source: electrophoretic mobility shift assay evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0413

Axiom
annotated source: electrophoretic mobility shift assay evidence
annotated property: has_related_synonym
annotated target: Gel retardation assay
database_cross_reference: PMID:17703195

cDNA microarray evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000097

in situ hybridization evidence
type: Class
subclass of: nucleic acid hybridization evidence
definition: Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence.
has_obo_namespace: eco
has_related_synonym: IDA: in situ hybridization
id: ECO:0000098

Axiom
annotated source: in situ hybridization evidence
annotated property: definition
annotated target: Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence.
database_cross_reference: TAIR:TED

fractionation evidence
type: Class
subclass of: direct assay evidence
has_obo_namespace: eco
id: ECO:0000100

Affymetrix array experiment evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000101

co-fractionation evidence
type: Class
subclass of: fractionation evidence
definition: Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules.
has_obo_namespace: eco
has_related_synonym: IDA: co-fractionation
id: ECO:0000102

Axiom
annotated source: co-fractionation evidence
annotated property: definition
annotated target: Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules.
database_cross_reference: TAIR:TED

microarray RNA expression level evidence
type: Class
subclass of: transcript expression level evidence
subclass of: expression microarray evidence
definition: Used when an annotation is made based on levels of RNA expression determined by microarray experiment.
has_obo_namespace: eco
has_related_synonym: IEP: transcript levels (e.g. microarray data)
id: ECO:0000104

Axiom
annotated source: microarray RNA expression level evidence
annotated property: definition
annotated target: Used when an annotation is made based on levels of RNA expression determined by microarray experiment.
database_cross_reference: TAIR:TED

Nimblegen array evidence
type: Class
subclass of: array experiment evidence
has_obo_namespace: eco
id: ECO:0000105

northern assay evidence
type: Class
subclass of: transcript expression level evidence
definition: A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples.
has_exact_synonym: Northern blot
has_obo_namespace: eco
has_related_synonym: IEP: transcript levels (e.g. Northerns)
id: ECO:0000106

Axiom
annotated source: northern assay evidence
annotated property: definition
annotated target: A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples.
database_cross_reference: ECO:SW
database_cross_reference: TAIR:TED

reverse transcription polymerase chain reaction transcription evidence
type: Class
subclass of: transcript expression level evidence
subclass of: reverse transcription polymerase chain reaction evidence
definition: Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise).
has_exact_synonym: RT-PCR transcription evidence
has_obo_namespace: eco
has_related_synonym: IEP: transcript levels (e.g. RT-PCR)
id: ECO:0000108

Axiom
annotated source: reverse transcription polymerase chain reaction transcription evidence
annotated property: definition
annotated target: Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise).
database_cross_reference: TAIR:TED

reverse transcription polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
definition: A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR.
has_exact_synonym: RT-PCR evidence
has_exact_synonym: qRT-PCR
has_obo_namespace: eco
id: ECO:0000109
comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate.

Axiom
annotated source: reverse transcription polymerase chain reaction evidence
annotated property: definition
annotated target: A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11013345
database_cross_reference: PMID:12901609

Axiom
annotated source: reverse transcription polymerase chain reaction evidence
annotated property: has_exact_synonym
annotated target: qRT-PCR
database_cross_reference: PMID:12901609

RNA protection assay evidence
type: Class
subclass of: nuclease protection assay evidence
definition: A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography.
has_exact_synonym: ribonuclease protection assay
has_narrow_synonym: RPA
has_obo_namespace: eco
has_related_synonym: IEP: RNA protection assay
has_related_synonym: RNAse protection assay
id: ECO:0000110

Axiom
annotated source: RNA protection assay evidence
annotated property: definition
annotated target: A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23457339
database_cross_reference: TAIR:TED

western blot evidence
type: Class
subclass of: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
subclass of: immunodetection assay evidence
definition: A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique.
has_obo_namespace: eco
has_related_synonym: IEP: protein levels (e.g. Western blots)
has_related_synonym: Western blot expression analysis
has_related_synonym: protein immunoblot
id: ECO:0000112
comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.

Axiom
annotated source: western blot evidence
annotated property: definition
annotated target: A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23050259
database_cross_reference: TAIR:TED

expression library screen evidence
type: Class
subclass of: protein expression evidence
definition: A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein.
has_obo_namespace: eco
has_related_synonym: IEP: expression library screening
id: ECO:0000114

Axiom
annotated source: expression library screen evidence
annotated property: definition
annotated target: A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED

differential hybridization evidence
type: Class
subclass of: transcript expression evidence
subclass of: nucleic acid hybridization evidence
definition: Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another.
has_obo_namespace: eco
has_related_synonym: IEP: differential hybridization
id: ECO:0000116

Axiom
annotated source: differential hybridization evidence
annotated property: definition
annotated target: Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another.
database_cross_reference: TAIR:TED

subtractive hybridization evidence
type: Class
subclass of: transcript expression evidence
subclass of: nucleic acid hybridization evidence
definition: Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another.
has_obo_namespace: eco
has_related_synonym: IEP: subtractive hybridization
id: ECO:0000118

Axiom
annotated source: subtractive hybridization evidence
annotated property: definition
annotated target: Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another.
database_cross_reference: TAIR:TED

over expression analysis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question.
has_obo_namespace: eco
has_related_synonym: IMP: analysis of overexpression/ectopic expression phenotype
id: ECO:0000120

Axiom
annotated source: over expression analysis evidence
annotated property: definition
annotated target: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question.
database_cross_reference: TAIR:TED

protein localization evidence
type: Class
subclass of: protein assay evidence
has_obo_namespace: eco
id: ECO:0000122

fusion protein localization evidence
type: Class
subclass of: protein localization evidence
has_obo_namespace: eco
id: ECO:0000124

green fluorescent protein fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
has_exact_synonym: GFP fusion protein localization evidence
has_obo_namespace: eco
has_related_synonym: IDA: localization of GFP/YFP fusion protein
id: ECO:0000126

yellow fluorescent protein fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
has_exact_synonym: YFP fusion protein localization evidence
has_obo_namespace: eco
has_related_synonym: IDA: localization of GFP/YFP fusion protein
id: ECO:0000128

beta-glucuronidase fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
has_exact_synonym: GUS fusion protein localization evidence
has_narrow_synonym: GUS staining evidence
has_obo_namespace: eco
has_related_synonym: IDA: localization of GUS fusion protein
id: ECO:0000130

beta-galactosidase fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
has_exact_synonym: LacZ fusion protein localization evidence
has_obo_namespace: eco
id: ECO:0000132

transport assay evidence
type: Class
subclass of: direct assay evidence
has_obo_namespace: eco
has_related_synonym: IDA: transport assay
id: ECO:0000134

nucleic acid binding evidence
type: Class
subclass of: affinity evidence
has_obo_namespace: eco
id: ECO:0000136

ribohomopolymer binding assay evidence
type: Class
subclass of: nucleic acid binding evidence
has_obo_namespace: eco
has_related_synonym: IDA: ribohomopolymer binding assay
id: ECO:0000138

thin layer chromatography evidence
type: Class
subclass of: chromatography evidence
definition: A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate.
has_exact_synonym: TLC evidence
has_obo_namespace: eco
has_related_synonym: IDA: thin layer chromatography
id: ECO:0000140

Axiom
annotated source: thin layer chromatography evidence
annotated property: definition
annotated target: A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate.
database_cross_reference: ECO:MCC

protein:ion binding evidence
type: Class
subclass of: protein binding evidence
has_obo_namespace: eco
id: ECO:0000142

Southwestern blot evidence
type: Class
subclass of: nucleic acid binding evidence
definition: Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins.
has_obo_namespace: eco
has_related_synonym: IDA: Southwestern analysis
id: ECO:0000144

Axiom
annotated source: Southwestern blot evidence
annotated property: definition
annotated target: Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins.
database_cross_reference: TAIR:TED

Northwestern blot evidence
type: Class
subclass of: nucleic acid binding evidence
definition: Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins.
has_obo_namespace: eco
has_related_synonym: IDA: Northwestern analysis
id: ECO:0000146

Axiom
annotated source: Northwestern blot evidence
annotated property: definition
annotated target: Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins.
database_cross_reference: TAIR:TED

in vitro binding evidence
type: Class
has_obo_namespace: eco
has_related_synonym: IDA: in vitro binding assay
id: ECO:0000148
deprecated: true

in vitro transcription reconstitution assay evidence
type: Class
subclass of: reconstitution assay evidence
has_obo_namespace: eco
has_related_synonym: IDA: in vitro reconstitution assay with recombinant protein
id: ECO:0000150

in vitro recombinant protein transcription reconstitution assay evidence
type: Class
subclass of: in vitro transcription reconstitution assay evidence
has_obo_namespace: eco
has_related_synonym: IDA: in vitro reconstitution assay with recombinant protein
id: ECO:0000152

heterologous protein expression evidence
type: Class
subclass of: protein expression evidence
definition: A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed.
has_obo_namespace: eco
has_related_synonym: IDA: protein expression in heterologous system
id: ECO:0000154

Axiom
annotated source: heterologous protein expression evidence
annotated property: definition
annotated target: A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed.
database_cross_reference: ECO:KAV

protein separation evidence
type: Class
subclass of: fractionation evidence
has_obo_namespace: eco
id: ECO:0000156

protein separation followed by direct sequencing evidence
type: Class
subclass of: protein separation evidence
has_obo_namespace: eco
has_related_synonym: IDA: protein separation and direct sequencing
id: ECO:0000158

protein separation followed by fragment identification evidence
type: Class
subclass of: protein separation evidence
has_obo_namespace: eco
has_related_synonym: IDA: protein separation and fragment identification
id: ECO:0000160

heterologous system uptake evidence
type: Class
subclass of: transport assay evidence
definition: Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein.
has_obo_namespace: eco
has_related_synonym: IDA: uptake assay in heterologous system
id: ECO:0000162

Axiom
annotated source: heterologous system uptake evidence
annotated property: definition
annotated target: Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein.
database_cross_reference: TAIR:TED

electrophysiology assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where electrical properties of cells or tissues are studied.
has_obo_namespace: eco
id: ECO:0000164
comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart.

Axiom
annotated source: electrophysiology assay evidence
annotated property: definition
annotated target: A type of direct assay evidence where electrical properties of cells or tissues are studied.
database_cross_reference: ECO:KAV

two-electrode voltage clamp experiment evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current.
has_obo_namespace: eco
has_related_synonym: IDA: two-electrode voltage clamp technique
id: ECO:0000166

Axiom
annotated source: two-electrode voltage clamp experiment evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current.
database_cross_reference: ECO:SN
database_cross_reference: PMID:23529422

transcription assay evidence
type: Class
subclass of: direct assay evidence
has_obo_namespace: eco
id: ECO:0000168

transcriptional activation assay
type: Class
subclass of: transcription assay evidence
definition: Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene.
has_obo_namespace: eco
has_related_synonym: IDA: transcriptional activation assay
id: ECO:0000170

Axiom
annotated source: transcriptional activation assay
annotated property: definition
annotated target: Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene.
database_cross_reference: TAIR:TED

biochemical trait analysis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate.
has_obo_namespace: eco
has_related_synonym: IMP: analysis of biochemical trait
id: ECO:0000172

Axiom
annotated source: biochemical trait analysis evidence
annotated property: definition
annotated target: Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate.
database_cross_reference: TAIR:TED

mutant physiological response evidence
type: Class
subclass of: mutant phenotype evidence
definition: Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions.
has_obo_namespace: eco
has_related_synonym: IMP: analysis of physiological response
id: ECO:0000174

Axiom
annotated source: mutant physiological response evidence
annotated property: definition
annotated target: Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions.
database_cross_reference: TAIR:TED

mutant visible phenotype evidence
type: Class
subclass of: mutant phenotype evidence
definition: Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology.
has_obo_namespace: eco
has_related_synonym: IMP: analysis of visible trait
id: ECO:0000176

Axiom
annotated source: mutant visible phenotype evidence
annotated property: definition
annotated target: Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology.
database_cross_reference: TAIR:TED

genomic context evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome.
has_obo_namespace: eco
id: ECO:0000177
comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis.

Axiom
annotated source: genomic context evidence
annotated property: definition
annotated target: An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go

in vivo assay evidence
type: Class
subclass of: biological assay evidence
has_obo_namespace: eco
id: ECO:0000178

animal model system study evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence arising from the investigation of an animal model system.
has_obo_namespace: eco
id: ECO:0000179

Axiom
annotated source: animal model system study evidence
annotated property: definition
annotated target: A type of experimental evidence arising from the investigation of an animal model system.
database_cross_reference: ECO:MCC

clinical study evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence arising from a controlled investigation that uses human subjects.
has_obo_namespace: eco
id: ECO:0000180

Axiom
annotated source: clinical study evidence
annotated property: definition
annotated target: A type of experimental evidence arising from a controlled investigation that uses human subjects.
database_cross_reference: ECO:MCC

in vitro assay evidence
type: Class
subclass of: biological assay evidence
has_obo_namespace: eco
id: ECO:0000181

in vitro culture assay evidence
type: Class
subclass of: biological assay evidence
has_obo_namespace: eco
id: ECO:0000182

cell-free assay evidence
type: Class
subclass of: in vitro assay evidence
has_broad_synonym: in vitro assay
has_obo_namespace: eco
id: ECO:0000183

enzyme inhibition evidence
type: Class
subclass of: enzyme assay evidence
subclass of: specific protein inhibition evidence
definition: A type of specific protein inhibition evidence where the protein under study is an enzyme.
has_obo_namespace: eco
id: ECO:0000184

Axiom
annotated source: enzyme inhibition evidence
annotated property: definition
annotated target: A type of specific protein inhibition evidence where the protein under study is an enzyme.
database_cross_reference: ECO:MCC

sequence alignment evidence
type: Class
subclass of: sequence similarity evidence
definition: A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments.
created by: mchibucos
creation date: 2010-03-18T12:21:31Z
has_alternative_id: ECO:00000057
has_obo_namespace: eco
id: ECO:0000200

Axiom
annotated source: sequence alignment evidence
annotated property: definition
annotated target: A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments.
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml

sequence orthology evidence
type: Class
subclass of: sequence similarity evidence
definition: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors.
created by: mchibucos
creation date: 2010-03-18T12:30:06Z
has_alternative_id: ECO:00000060
has_obo_namespace: eco
has_related_synonym: ortholog evidence
id: ECO:0000201

Axiom
annotated source: sequence orthology evidence
annotated property: definition
annotated target: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors.
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml

match to sequence model evidence
type: Class
subclass of: sequence similarity evidence
definition: Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product.
created by: mchibucos
creation date: 2010-03-18T12:32:30Z
has_alternative_id: ECO:00000063
has_obo_namespace: eco
id: ECO:0000202
comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN.

Axiom
annotated source: match to sequence model evidence
annotated property: definition
annotated target: Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product.
database_cross_reference: ECO:MCC
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml

automatic assertion
type: Class
subclass of: assertion method
disjoint with: manual assertion
definition: An assertion method that does not involve human review.
created by: mchibucos
creation date: 2010-03-18T12:36:04Z
has_alternative_id: ECO:00000067
has_obo_namespace: eco
id: ECO:0000203
comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically.

Axiom
annotated source: automatic assertion
annotated property: definition
annotated target: An assertion method that does not involve human review.
database_cross_reference: ECO:MCC

author statement
type: Class
equivalent class: Class
  union of: traceable author statement
  union of: non-traceable author statement
subclass of: evidence
definition: An evidence type that is based on an assertion by the author of a paper, which is read by a curator.
created by: mchibucos
creation date: 2010-06-21T11:17:21Z
has_obo_namespace: eco
id: ECO:0000204

Axiom
annotated source: author statement
annotated property: definition
annotated target: An evidence type that is based on an assertion by the author of a paper, which is read by a curator.
database_cross_reference: ECO:MCC

curator inference
type: Class
subclass of: inferential evidence
definition: A type of inferential evidence that is based on a conclusion drawn by a curator.
created by: mchibucos
creation date: 2010-08-18T05:55:12Z
has_obo_namespace: eco
id: ECO:0000205

Axiom
annotated source: curator inference
annotated property: definition
annotated target: A type of inferential evidence that is based on a conclusion drawn by a curator.
database_cross_reference: ECO:MCC

BLAST evidence
type: Class
subclass of: pairwise sequence alignment evidence
definition: A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST).
created by: mchibucos
creation date: 2010-08-06T04:48:24Z
has_obo_namespace: eco
id: ECO:0000206

Axiom
annotated source: BLAST evidence
annotated property: definition
annotated target: A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST).
database_cross_reference: ECO:MCC

nucleotide BLAST evidence
type: Class
subclass of: BLAST evidence
definition: BLAST evidence from aligning nucleotide sequence with nucleotide sequence.
created by: mchibucos
creation date: 2010-08-06T04:49:58Z
has_obo_namespace: eco
id: ECO:0000207

Axiom
annotated source: nucleotide BLAST evidence
annotated property: definition
annotated target: BLAST evidence from aligning nucleotide sequence with nucleotide sequence.
database_cross_reference: ECO:MCC

protein BLAST evidence
type: Class
subclass of: BLAST evidence
definition: BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence.
created by: mchibucos
creation date: 2010-08-06T04:50:45Z
has_obo_namespace: eco
id: ECO:0000208

Axiom
annotated source: protein BLAST evidence
annotated property: definition
annotated target: BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence.
database_cross_reference: ECO:MCC

BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: BLAST evidence
subclass of: pairwise sequence alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of BLAST evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-08-06T04:55:21Z
has_obo_namespace: eco
id: ECO:0000209

Axiom
annotated source: BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: pairwise sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: BLAST evidence used in automatic assertion
annotated property: definition
annotated target: A type of BLAST evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

nucleotide BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: nucleotide BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: nucleotide BLAST evidence
subclass of: BLAST evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Nucleotide BLAST evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-08-06T05:09:51Z
has_obo_namespace: eco
id: ECO:0000210

Axiom
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: BLAST evidence used in automatic assertion
is inferred: true

Axiom
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: definition
annotated target: Nucleotide BLAST evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

protein BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: protein BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: protein BLAST evidence
subclass of: BLAST evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Protein BLAST evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-08-06T05:11:04Z
has_obo_namespace: eco
id: ECO:0000211

Axiom
annotated source: protein BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: BLAST evidence used in automatic assertion
is inferred: true

Axiom
annotated source: protein BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: protein BLAST evidence used in automatic assertion
annotated property: definition
annotated target: Protein BLAST evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

combinatorial evidence
type: Class
subclass of: evidence
definition: A type of evidence that arises out of the integration of more than one line of evidence.
created by: mchibucos
creation date: 2010-08-06T05:24:57Z
has_alternative_id: ECO:0000036
has_alternative_id: ECO:0000043
has_obo_namespace: eco
has_related_synonym: inferred from in-silico analysis
id: ECO:0000212
comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence.

Axiom
annotated source: combinatorial evidence
annotated property: definition
annotated target: A type of evidence that arises out of the integration of more than one line of evidence.
database_cross_reference: ECO:MCC

combinatorial evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: combinatorial evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of combinatorial analysis that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-08-06T05:28:33Z
has_obo_namespace: eco
id: ECO:0000213
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.

Axiom
annotated source: combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: combinatorial evidence used in automatic assertion
annotated property: definition
annotated target: A type of combinatorial analysis that is used in an automatic assertion.
database_cross_reference: ECO:MCC

biological aspect of descendant evidence
type: Class
subclass of: phylogenetic evidence
definition: A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene.
created by: mchibucos
creation date: 2010-10-05T11:16:50Z
has_obo_namespace: eco
id: ECO:0000214
comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones.

Axiom
annotated source: biological aspect of descendant evidence
annotated property: definition
annotated target: A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene.
database_cross_reference: ECO:MCC

rapid divergence from ancestral sequence evidence
type: Class
subclass of: phylogenetic evidence
definition: A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event.
created by: mchibucos
creation date: 2010-10-05T03:41:34Z
has_obo_namespace: eco
id: ECO:0000215
comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence.

Axiom
annotated source: rapid divergence from ancestral sequence evidence
annotated property: definition
annotated target: A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event.
database_cross_reference: ECO:MCC

phylogenetic determination of loss of key residues evidence
type: Class
subclass of: phylogenetic evidence
definition: A type of phylogenetic evidence characterized by the absence of key sequence residues.
created by: mchibucos
creation date: 2010-10-05T03:43:17Z
has_obo_namespace: eco
id: ECO:0000216
comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function.

Axiom
annotated source: phylogenetic determination of loss of key residues evidence
annotated property: definition
annotated target: A type of phylogenetic evidence characterized by the absence of key sequence residues.
database_cross_reference: ECO:MCC

assertion method
type: Class
equivalent class: Class
  union of: automatic assertion
  union of: manual assertion
definition: A means by which a statement is made about an entity.
created by: mchibucos
creation date: 2010-11-12T04:17:37Z
has_obo_namespace: eco
id: ECO:0000217

Axiom
annotated source: assertion method
annotated property: definition
annotated target: A means by which a statement is made about an entity.
database_cross_reference: ECO:MCC

manual assertion
type: Class
subclass of: assertion method
definition: An assertion method that involves human review.
created by: mchibucos
creation date: 2010-11-12T04:20:02Z
has_obo_namespace: eco
id: ECO:0000218
comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information.

Axiom
annotated source: manual assertion
annotated property: definition
annotated target: An assertion method that involves human review.
database_cross_reference: ECO:MCC

nucleotide sequencing assay evidence
type: Class
subclass of: sequencing assay evidence
definition: A sequencing assay result for a biopolymer that comprises nucleotides.
created by: mchibucos
creation date: 2010-11-15T12:22:40Z
has_obo_namespace: eco
has_related_synonym: DNA sequencing
id: ECO:0000219

Axiom
annotated source: nucleotide sequencing assay evidence
annotated property: definition
annotated target: A sequencing assay result for a biopolymer that comprises nucleotides.
database_cross_reference: ECO:MCC

Axiom
annotated source: nucleotide sequencing assay evidence
annotated property: has_related_synonym
annotated target: DNA sequencing
database_cross_reference: OBI:0000626

sequencing assay evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence where the order of molecules that constitute a biopolymer is determined.
created by: mchibucos
creation date: 2010-11-15T01:44:45Z
has_obo_namespace: eco
id: ECO:0000220

Axiom
annotated source: sequencing assay evidence
annotated property: definition
annotated target: A type of experimental evidence where the order of molecules that constitute a biopolymer is determined.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0600047

high throughput nucleotide sequencing assay evidence
type: Class
subclass of: nucleotide sequencing assay evidence
definition: A nucleotide sequencing assay result that is derived from a parallelized sequencing technique.
created by: mchibucos
creation date: 2010-11-15T02:19:34Z
has_obo_namespace: eco
id: ECO:0000221
comment: Typically thousands to millions of reads are produced in parallel.

Axiom
annotated source: high throughput nucleotide sequencing assay evidence
annotated property: definition
annotated target: A nucleotide sequencing assay result that is derived from a parallelized sequencing technique.
database_cross_reference: ECO:MCC

Illumina sequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
definition: A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.
created by: mchibucos
creation date: 2010-11-15T02:38:03Z
has_obo_namespace: eco
has_related_synonym: Solexa sequencing result
id: ECO:0000222
comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging.

Axiom
annotated source: Illumina sequencing evidence
annotated property: definition
annotated target: A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.
database_cross_reference: ECO:MCC

Axiom
annotated source: Illumina sequencing evidence
annotated property: has_related_synonym
annotated target: Solexa sequencing result
database_cross_reference: OBI:0000724

454 pyrosequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
definition: A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology.
created by: mchibucos
creation date: 2010-11-15T03:50:14Z
has_broad_synonym: pyrosequencing
has_obo_namespace: eco
id: ECO:0000223
comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.

Axiom
annotated source: 454 pyrosequencing evidence
annotated property: definition
annotated target: A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology.
database_cross_reference: ECO:MCC

Axiom
annotated source: 454 pyrosequencing evidence
annotated property: has_broad_synonym
annotated target: pyrosequencing
database_cross_reference: OBI:0000730

SOLiD sequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
definition: A high throughput nucleotide sequencing result derived by SOLiD sequencing technology.
created by: mchibucos
creation date: 2010-11-15T04:17:41Z
has_obo_namespace: eco
id: ECO:0000224
comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag.

Axiom
annotated source: SOLiD sequencing evidence
annotated property: definition
annotated target: A high throughput nucleotide sequencing result derived by SOLiD sequencing technology.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0000706

chain termination sequencing evidence
type: Class
subclass of: nucleotide sequencing assay evidence
definition: A nucleotide sequencing assay result derived by the chain termination sequencing method.
created by: mchibucos
creation date: 2010-11-15T04:46:31Z
has_exact_synonym: Sanger sequencing
has_exact_synonym: dye terminator sequencing
has_obo_namespace: eco
id: ECO:0000225
comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.

Axiom
annotated source: chain termination sequencing evidence
annotated property: definition
annotated target: A nucleotide sequencing assay result derived by the chain termination sequencing method.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0000695

chromatin immunoprecipitation evidence
type: Class
subclass of: immunoprecipitation evidence
definition: A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence.
created by: mchibucos
creation date: 2010-11-16T01:29:08Z
database_cross_reference: PSI-MI:MI:0402
has_exact_synonym: ChIP evidence
has_obo_namespace: eco
id: ECO:0000226
comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).

Axiom
annotated source: chromatin immunoprecipitation evidence
annotated property: definition
annotated target: A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence.
database_cross_reference: ECO:MCC

chromatin immunoprecipitation assay
type: Axiom
annotated source: chromatin immunoprecipitation evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0402

chromatin immunoprecipitation-PCR evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
definition: A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection.
created by: mchibucos
creation date: 2010-11-16T05:02:00Z
has_exact_synonym: ChIP-PCR evidence
has_obo_namespace: eco
id: ECO:0000227
comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results.

Axiom
annotated source: chromatin immunoprecipitation-PCR evidence
annotated property: definition
annotated target: A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:18388953

chromatin immunoprecipitation-qPCR evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
definition: A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection.
created by: mchibucos
creation date: 2010-11-16T05:26:47Z
has_exact_synonym: ChIP-qPCR evidence
has_obo_namespace: eco
id: ECO:0000228

Axiom
annotated source: chromatin immunoprecipitation-qPCR evidence
annotated property: definition
annotated target: A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection.
database_cross_reference: ECO:MCC

chromatin immunoprecipitation-seq evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
definition: A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins.
created by: mchibucos
creation date: 2010-11-16T05:30:41Z
has_exact_synonym: ChIP-SEQ evidence
has_exact_synonym: ChIP-seq evidence
has_obo_namespace: eco
id: ECO:0000229
comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used.

Axiom
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: definition
annotated target: A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: OBI:0000716
database_cross_reference: PMID:22955991

Axiom
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: has_exact_synonym
annotated target: ChIP-SEQ evidence
database_cross_reference: OBI:0000716

Axiom
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: has_exact_synonym
annotated target: ChIP-seq evidence
database_cross_reference: PMID:22955991

chromatin immunoprecipitation-chip evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
definition: A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions.
created by: mchibucos
creation date: 2010-11-16T05:43:55Z
database_cross_reference: PSI-MI:MI:0225
has_exact_synonym: ChIP-chip evidence
has_exact_synonym: ChIP-on-chip evidence
has_obo_namespace: eco
id: ECO:0000230
comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp.

Axiom
annotated source: chromatin immunoprecipitation-chip evidence
annotated property: definition
annotated target: A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:18388953
database_cross_reference: PMID:19381927

chromatin immunoprecipitation array
type: Axiom
annotated source: chromatin immunoprecipitation-chip evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0225

quantitative polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
definition: A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample.
created by: mchibucos
creation date: 2010-11-16T05:57:20Z
has_exact_synonym: Q-PCR evidence
has_exact_synonym: quantitative PCR evidence
has_exact_synonym: real-time PCR evidence
has_exact_synonym: real-time polymerase chain reaction evidence
has_obo_namespace: eco
has_related_synonym: qRT-PCR evidence
id: ECO:0000231
comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA. Although qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).

Axiom
annotated source: quantitative polymerase chain reaction evidence
annotated property: definition
annotated target: A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW

chromosome conformation capture-based evidence
type: Class
subclass of: experimental genomic evidence
definition: A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation.
created by: mchibucos
creation date: 2010-11-17T12:27:14Z
has_narrow_synonym: 3C
has_narrow_synonym: chromosome conformation capture
has_obo_namespace: eco
id: ECO:0000232
comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C.

Axiom
annotated source: chromosome conformation capture-based evidence
annotated property: definition
annotated target: A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation.
database_cross_reference: ECO:MCC

chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
definition: A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step.
created by: mchibucos
creation date: 2010-11-17T12:46:15Z
has_exact_synonym: 3C
has_obo_namespace: eco
id: ECO:0000233
comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation.

Axiom
annotated source: chromosome conformation capture evidence
annotated property: definition
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step.
database_cross_reference: ECO:MCC

circularized chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
definition: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray.
created by: mchibucos
creation date: 2010-11-19T01:20:28Z
has_exact_synonym: 4C
has_exact_synonym: chromosome conformation capture on chip
has_exact_synonym: circularized 3C
has_obo_namespace: eco
id: ECO:0000234
comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation.

Axiom
annotated source: circularized chromosome conformation capture evidence
annotated property: definition
annotated target: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray.
database_cross_reference: ECO:MCC

carbon-copy chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
definition: A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation.
created by: mchibucos
creation date: 2010-11-19T01:22:20Z
has_exact_synonym: 5C
has_exact_synonym: carbon-copy 3C
has_obo_namespace: eco
id: ECO:0000235
comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation.

Axiom
annotated source: carbon-copy chromosome conformation capture evidence
annotated property: definition
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation.
database_cross_reference: ECO:MCC

chromosome conformation capture-PCR evidence
type: Class
subclass of: chromosome conformation capture evidence
definition: A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step.
created by: mchibucos
creation date: 2010-11-19T03:00:47Z
has_broad_synonym: 3C
has_exact_synonym: 3C-PCR
has_obo_namespace: eco
id: ECO:0000236

Axiom
annotated source: chromosome conformation capture-PCR evidence
annotated property: definition
annotated target: A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step.
database_cross_reference: ECO:MCC

chromosome conformation capture-qPCR evidence
type: Class
subclass of: chromosome conformation capture evidence
definition: A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step.
created by: mchibucos
creation date: 2010-11-19T03:06:47Z
has_exact_synonym: 3C-qPCR
has_exact_synonym: 3C-quantitative PCR
has_exact_synonym: 3C-real-time PCR
has_obo_namespace: eco
id: ECO:0000237

Axiom
annotated source: chromosome conformation capture-qPCR evidence
annotated property: definition
annotated target: A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step.
database_cross_reference: ECO:MCC

Hi-C evidence
type: Class
subclass of: chromosome conformation capture-based evidence
definition: A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation.
created by: mchibucos
creation date: 2010-11-19T04:24:03Z
has_obo_namespace: eco
id: ECO:0000238
comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step.

Axiom
annotated source: Hi-C evidence
annotated property: definition
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation.
database_cross_reference: ECO:MCC

chromosome conformation capture sequencing evidence
type: Class
subclass of: chromosome conformation capture-based evidence
definition: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing.
created by: mchibucos
creation date: 2010-11-30T03:22:25Z
has_exact_synonym: 3C sequencing
has_exact_synonym: 3C-seq
has_exact_synonym: 4C sequencing
has_exact_synonym: 4C-seq
has_exact_synonym: circularized 3C sequencing
has_exact_synonym: circularized 3C-seq
has_obo_namespace: eco
id: ECO:0000239
comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation.

Axiom
annotated source: chromosome conformation capture sequencing evidence
annotated property: definition
annotated target: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing.
database_cross_reference: ECO:MCC

anatomical perturbation evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Experimental phenotypic evidence where a structural feature of an organism is disrupted.
created by: mchibucos
creation date: 2010-12-06T12:24:16Z
has_obo_namespace: eco
id: ECO:0000240

Axiom
annotated source: anatomical perturbation evidence
annotated property: definition
annotated target: Experimental phenotypic evidence where a structural feature of an organism is disrupted.
database_cross_reference: ECO:MCC

environmental perturbation evidence
type: Class
subclass of: experimental phenotypic evidence
definition: Experimental phenotypic evidence where the extra-organismal context of an organism is modified.
created by: mchibucos
creation date: 2010-12-06T12:33:58Z
has_obo_namespace: eco
has_related_synonym: mechanical constraint evidence
id: ECO:0000241
comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth.

Axiom
annotated source: environmental perturbation evidence
annotated property: definition
annotated target: Experimental phenotypic evidence where the extra-organismal context of an organism is modified.
database_cross_reference: ECO:MCC

tissue ablation evidence
type: Class
subclass of: anatomical perturbation evidence
definition: A type of anatomical perturbation that is based on disruption of tissue.
created by: mchibucos
creation date: 2010-12-06T12:33:58Z
has_exact_synonym: ablated tissue evidence
has_obo_namespace: eco
id: ECO:0000242
comment: A tissue ablation experiment involves observing the phenotype of an entity whose tissues have been disrupted through genetic or biochemical manipulation, physical destruction, or some other means.

Axiom
annotated source: tissue ablation evidence
annotated property: definition
annotated target: A type of anatomical perturbation that is based on disruption of tissue.
database_cross_reference: ECO:MCC

tissue grafting evidence
type: Class
subclass of: anatomical perturbation evidence
definition: A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure.
created by: mchibucos
creation date: 2010-12-06T12:33:58Z
has_obo_namespace: eco
id: ECO:0000243

Axiom
annotated source: tissue grafting evidence
annotated property: definition
annotated target: A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure.
database_cross_reference: ECO:MCC

combinatorial evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: combinatorial evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of combinatorial analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-09T02:02:12Z
has_obo_namespace: eco
id: ECO:0000244
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.

Axiom
annotated source: combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: combinatorial evidence used in manual assertion
annotated property: definition
annotated target: A type of combinatorial analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

computational combinatorial evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: computational combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: computational combinatorial evidence
subclass of: combinatorial evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of computational combinatorial analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-09T02:37:54Z
database_cross_reference: GOECO:RCA
has_exact_synonym: RCA
has_exact_synonym: inferred from reviewed computational analysis
has_obo_namespace: eco
id: ECO:0000245
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: combinatorial evidence used in manual assertion
is inferred: true

Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: definition
annotated target: A type of computational combinatorial analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from reviewed computational analysis
type: Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:RCA

Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: RCA
database_cross_reference: GOECO:RCA

Axiom
annotated source: computational combinatorial evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from reviewed computational analysis
database_cross_reference: GOECO:RCA

computational combinatorial evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: computational combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: computational combinatorial evidence
subclass of: combinatorial evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of computational combinatorial analysis that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-12-09T02:37:54Z
has_obo_namespace: eco
id: ECO:0000246

Axiom
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: combinatorial evidence used in automatic assertion
is inferred: true

Axiom
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: definition
annotated target: A type of computational combinatorial analysis that is used in an automatic assertion.
database_cross_reference: ECO:MCC

sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Sequence alignment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-09T05:12:27Z
database_cross_reference: GOECO:ISA
has_exact_synonym: ISA
has_exact_synonym: inferred from sequence alignment
has_obo_namespace: eco
id: ECO:0000247
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: definition
annotated target: Sequence alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from sequence alignment
type: Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:ISA

Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: ISA
database_cross_reference: GOECO:ISA

Axiom
annotated source: sequence alignment evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from sequence alignment
database_cross_reference: GOECO:ISA

sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Sequence alignment evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-12-09T05:12:27Z
has_obo_namespace: eco
id: ECO:0000248

Axiom
annotated source: sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: sequence alignment evidence used in automatic assertion
annotated property: definition
annotated target: Sequence alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

sequence similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence similarity evidence
subclass of: similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Sequence similarity evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-12-09T05:22:30Z
has_obo_namespace: eco
id: ECO:0000249

Axiom
annotated source: sequence similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: sequence similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: sequence similarity evidence used in automatic assertion
annotated property: definition
annotated target: Sequence similarity evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

sequence similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence similarity evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of sequence similarity evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-09T05:22:30Z
database_cross_reference: GOECO:ISS
has_exact_synonym: ISS
has_exact_synonym: inferred from sequence or structural similarity
has_obo_namespace: eco
id: ECO:0000250
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: definition
annotated target: A type of sequence similarity evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from sequence or structural similarity
type: Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:ISS

Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: ISS
database_cross_reference: GOECO:ISS

Axiom
annotated source: sequence similarity evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from sequence or structural similarity
database_cross_reference: GOECO:ISS

similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: similarity evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Similarity evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-12-09T05:28:12Z
has_obo_namespace: eco
id: ECO:0000251

Axiom
annotated source: similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: similarity evidence used in automatic assertion
annotated property: definition
annotated target: Similarity evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: similarity evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Similarity evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-09T05:28:12Z
has_obo_namespace: eco
id: ECO:0000252
in_subset: go groupings

Axiom
annotated source: similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: similarity evidence used in manual assertion
annotated property: definition
annotated target: Similarity evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

genetic similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetic similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genetic similarity evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Genetic similarity evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T01:42:44Z
has_obo_namespace: eco
id: ECO:0000253

Axiom
annotated source: genetic similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetic similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: genetic similarity evidence used in manual assertion
annotated property: definition
annotated target: Genetic similarity evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

genetic similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: genetic similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: genetic similarity evidence
subclass of: similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Genetic similarity evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T01:42:44Z
has_obo_namespace: eco
id: ECO:0000254

Axiom
annotated source: genetic similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: genetic similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: genetic similarity evidence used in automatic assertion
annotated property: definition
annotated target: Genetic similarity evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

match to sequence model evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: match to sequence model evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: match to sequence model evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A match to sequence model evidence that is employed in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T02:26:35Z
database_cross_reference: GOECO:ISM
database_cross_reference: GO_REF:0000011
has_exact_synonym: ISM
has_exact_synonym: inferred from sequence model
has_obo_namespace: eco
id: ECO:0000255

Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: definition
annotated target: A match to sequence model evidence that is employed in a manual assertion.
database_cross_reference: ECO:MCC

inferred from sequence model
type: Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:ISM

Hidden Markov Models (TIGR)
type: Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000011

Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: ISM
database_cross_reference: GOECO:ISM

Axiom
annotated source: match to sequence model evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from sequence model
database_cross_reference: GOECO:ISM

match to sequence model evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: match to sequence model evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: match to sequence model evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A match to sequence model evidence that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T02:26:35Z
database_cross_reference: GO_REF:0000002
database_cross_reference: GO_REF:0000107
has_obo_namespace: eco
id: ECO:0000256

Axiom
annotated source: match to sequence model evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: match to sequence model evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: match to sequence model evidence used in automatic assertion
annotated property: definition
annotated target: A match to sequence model evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

Gene Ontology annotation through association of InterPro records with GO terms.
type: Axiom
annotated source: match to sequence model evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000002

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl
type: Axiom
annotated source: match to sequence model evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000107

motif similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motif similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence
subclass of: match to sequence model evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Motif similarity evidence that is employed in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T02:44:34Z
has_obo_namespace: eco
id: ECO:0000257

Axiom
annotated source: motif similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in manual assertion
is inferred: true

Axiom
annotated source: motif similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: motif similarity evidence used in manual assertion
annotated property: definition
annotated target: Motif similarity evidence that is employed in a manual assertion.
database_cross_reference: ECO:MCC

motif similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: motif similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence
subclass of: match to sequence model evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Motif similarity evidence that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T02:44:34Z
has_obo_namespace: eco
id: ECO:0000258

Axiom
annotated source: motif similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in automatic assertion
is inferred: true

Axiom
annotated source: motif similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: motif similarity evidence used in automatic assertion
annotated property: definition
annotated target: Motif similarity evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

match to InterPro member signature evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: match to InterPro member signature evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: match to InterPro member signature evidence
subclass of: match to sequence model evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A match to InterPro member signature evidence that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T02:54:51Z
has_obo_namespace: eco
id: ECO:0000259

Axiom
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in automatic assertion
is inferred: true

Axiom
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: definition
annotated target: A match to InterPro member signature evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

match to InterPro member signature evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: match to InterPro member signature evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: match to InterPro member signature evidence
subclass of: match to sequence model evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A match to InterPro member signature evidence that is employed in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T02:54:51Z
has_obo_namespace: eco
id: ECO:0000260

Axiom
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in manual assertion
is inferred: true

Axiom
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: definition
annotated target: A match to InterPro member signature evidence that is employed in a manual assertion.
database_cross_reference: ECO:MCC

targeting sequence prediction evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: targeting sequence prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: targeting sequence prediction evidence
subclass of: motif similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A targeting sequence prediction that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T03:02:49Z
has_obo_namespace: eco
id: ECO:0000261

Axiom
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: definition
annotated target: A targeting sequence prediction that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

targeting sequence prediction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: targeting sequence prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: targeting sequence prediction evidence
subclass of: motif similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A targeting sequence prediction that is employed in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T03:02:49Z
has_obo_namespace: eco
id: ECO:0000262

Axiom
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: definition
annotated target: A targeting sequence prediction that is employed in a manual assertion.
database_cross_reference: ECO:MCC

transmembrane domain prediction evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: transmembrane domain prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: transmembrane domain prediction evidence
subclass of: motif similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Transmembrane domain prediction that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T03:28:35Z
has_obo_namespace: eco
id: ECO:0000263

Axiom
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: definition
annotated target: Transmembrane domain prediction that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

transmembrane domain prediction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transmembrane domain prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transmembrane domain prediction evidence
subclass of: motif similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: Transmembrane domain prediction that is employed in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T03:28:35Z
has_obo_namespace: eco
id: ECO:0000264

Axiom
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: definition
annotated target: Transmembrane domain prediction that is employed in a manual assertion.
database_cross_reference: ECO:MCC

sequence orthology evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence orthology evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: Sequence orthology evidence that is employed in an automatic assertion.
created by: mchibucos
creation date: 2010-12-10T03:39:41Z
database_cross_reference: GO_REF:0000019
database_cross_reference: GO_REF:0000020
database_cross_reference: GO_REF:0000035
database_cross_reference: GO_REF:0000049
has_obo_namespace: eco
has_related_synonym: Ortholog evidence
id: ECO:0000265

Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: definition
annotated target: Sequence orthology evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
type: Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000019

Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins.
type: Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000020

Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara
type: Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000035

Automatic transfer of experimentally verified manual GO annotation data to fungal orthologs using Ensembl Compara
type: Axiom
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000049

sequence orthology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence orthology evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of sequence orthology evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-10T03:39:41Z
database_cross_reference: GOECO:ISO
has_exact_synonym: ISO
has_exact_synonym: inferred from sequence orthology
has_obo_namespace: eco
has_related_synonym: Ortholog evidence
id: ECO:0000266
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: definition
annotated target: A type of sequence orthology evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from sequence orthology
type: Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:ISO

Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: ISO
database_cross_reference: GOECO:ISO

GOECO:ISO
type: Axiom
annotated source: sequence orthology evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from sequence orthology

enzyme-linked immunoabsorbent assay evidence
type: Class
subclass of: protein detection assay evidence
definition: A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate.
created by: mchibucos
creation date: 2010-12-14T01:57:13Z
database_cross_reference: PSI-MI:MI:0411
has_exact_synonym: ELISA evidence
has_narrow_synonym: enzyme-linked immunosorbent assay
has_obo_namespace: eco
id: ECO:0000267
comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein.

Axiom
annotated source: enzyme-linked immunoabsorbent assay evidence
annotated property: definition
annotated target: A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:23949770

enzyme linked immunosorbent assay
type: Axiom
annotated source: enzyme-linked immunoabsorbent assay evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0411

flow cytometry evidence
type: Class
subclass of: direct assay evidence
definition: A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles.
created by: mchibucos
creation date: 2010-12-14T02:07:08Z
has_exact_synonym: FCM
has_obo_namespace: eco
id: ECO:0000268

Axiom
annotated source: flow cytometry evidence
annotated property: definition
annotated target: A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles.
database_cross_reference: ECO:MCC

experimental evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: experimental evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of experimental evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:36:49Z
database_cross_reference: GOECO:EXP
has_exact_synonym: EXP
has_exact_synonym: inferred from experiment
has_obo_namespace: eco
id: ECO:0000269

Axiom
annotated source: experimental evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: experimental evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: experimental evidence used in manual assertion
annotated property: definition
annotated target: A type of experimental evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from experiment
type: Axiom
annotated source: experimental evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:EXP

Axiom
annotated source: experimental evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: EXP
database_cross_reference: GOECO:EXP

Axiom
annotated source: experimental evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from experiment
database_cross_reference: GOECO:EXP

expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression pattern evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of expression pattern evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:46:39Z
database_cross_reference: GOECO:IEP
has_exact_synonym: IEP
has_exact_synonym: inferred from expression pattern
has_obo_namespace: eco
id: ECO:0000270

Axiom
annotated source: expression evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: expression evidence used in manual assertion
annotated property: definition
annotated target: A type of expression pattern evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from expression pattern
type: Axiom
annotated source: expression evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IEP

Axiom
annotated source: expression evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IEP
database_cross_reference: GOECO:IEP

Axiom
annotated source: expression evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from expression pattern
database_cross_reference: GOECO:IEP

array experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: array experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: array experiment evidence
subclass of: expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of array experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:51:58Z
has_obo_namespace: eco
id: ECO:0000271

Axiom
annotated source: array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: array experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of array experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Affymetrix array experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Affymetrix array experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Affymetrix array experiment evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Affymetrix array experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:53:36Z
has_obo_namespace: eco
id: ECO:0000272

Axiom
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of Affymetrix array experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

cDNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cDNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: cDNA microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:55:49Z
has_obo_namespace: eco
id: ECO:0000273

Axiom
annotated source: cDNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: cDNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cDNA microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

CpG island microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: CpG island microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: CpG island microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T05:58:44Z
has_obo_namespace: eco
id: ECO:0000274

Axiom
annotated source: CpG island microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: CpG island microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: CpG island microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

expression microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of expression microarray evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:01:26Z
has_obo_namespace: eco
id: ECO:0000275

Axiom
annotated source: expression microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: expression microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: expression microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of expression microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

genomic microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genomic microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genomic microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence arising from a genomic microarray experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:03:28Z
has_obo_namespace: eco
id: ECO:0000276

Axiom
annotated source: genomic microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: genomic microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: genomic microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence arising from a genomic microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

Nimblegen array evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Nimblegen array evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Nimblegen array evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:05:20Z
has_obo_namespace: eco
id: ECO:0000277

Axiom
annotated source: Nimblegen array evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: Nimblegen array evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Nimblegen array evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

oligonucleotide microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: oligonucleotide microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: oligonucleotide microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:09:45Z
has_obo_namespace: eco
id: ECO:0000278

Axiom
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

western blot evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: western blot evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: western blot evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Western blot assay that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:20:01Z
has_obo_namespace: eco
id: ECO:0000279

Axiom
annotated source: western blot evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: western blot evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: western blot evidence used in manual assertion
annotated property: definition
annotated target: A type of Western blot assay that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: protein expression level evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein expression level analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:21:54Z
has_obo_namespace: eco
id: ECO:0000280

Axiom
annotated source: protein expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: protein expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein expression level evidence used in manual assertion
annotated property: definition
annotated target: A type of protein expression level analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

expression library screen evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression library screen evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression library screen evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of expression library screen evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:24:05Z
has_obo_namespace: eco
id: ECO:0000281

Axiom
annotated source: expression library screen evidence used in manual assertion
annotated property: subClassOf
annotated target: protein expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: expression library screen evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: expression library screen evidence used in manual assertion
annotated property: definition
annotated target: A type of expression library screen evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

heterologous protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: heterologous protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: heterologous protein expression evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of heterologous protein expression analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:27:04Z
has_obo_namespace: eco
id: ECO:0000282

Axiom
annotated source: heterologous protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: protein expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: heterologous protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: heterologous protein expression evidence used in manual assertion
annotated property: definition
annotated target: A type of heterologous protein expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

spatial pattern of protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spatial pattern of protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: spatial pattern of protein expression evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein expression spatial pattern analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:31:44Z
has_obo_namespace: eco
id: ECO:0000283

Axiom
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: protein expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated property: definition
annotated target: A type of protein expression spatial pattern analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: protein expression evidence
subclass of: expression evidence used in manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein expression analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:33:46Z
has_obo_namespace: eco
id: ECO:0000284

Axiom
annotated source: protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein expression evidence used in manual assertion
annotated property: definition
annotated target: A type of protein expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

microarray RNA expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: microarray RNA expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microarray RNA expression level evidence
subclass of: expression microarray evidence used in manual assertion
subclass of: transcript expression level evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of microarray RNA expression level data that is used in manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:41:33Z
has_obo_namespace: eco
id: ECO:0000285

Axiom
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: expression microarray evidence used in manual assertion
is inferred: true

Axiom
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression level evidence used in manual assertion
is inferred: true

Axiom
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: definition
annotated target: A type of microarray RNA expression level data that is used in manual assertion.
database_cross_reference: ECO:MCC

transcript expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transcript expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression level evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of transcript expression level data that is used in manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:52:03Z
has_obo_namespace: eco
id: ECO:0000286

Axiom
annotated source: transcript expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: transcript expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: transcript expression level evidence used in manual assertion
annotated property: definition
annotated target: A type of transcript expression level data that is used in manual assertion.
database_cross_reference: ECO:MCC

differential hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: differential hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: differential hybridization evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of differential hybridization experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:55:14Z
has_obo_namespace: eco
id: ECO:0000287

Axiom
annotated source: differential hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: differential hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: differential hybridization evidence used in manual assertion
annotated property: definition
annotated target: A type of differential hybridization experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

RNA protection assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA protection assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: RNA protection assay evidence
subclass of: nuclease protection assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of RNA protection assay that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:57:33Z
has_obo_namespace: eco
id: ECO:0000288

Axiom
annotated source: RNA protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: nuclease protection assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: RNA protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: RNA protection assay evidence used in manual assertion
annotated property: definition
annotated target: A type of RNA protection assay that is used in a manual assertion.
database_cross_reference: ECO:MCC

spatial pattern of transcript expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spatial pattern of transcript expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: spatial pattern of transcript expression evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of spatial pattern of transcript expression analysis that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T06:59:18Z
has_obo_namespace: eco
id: ECO:0000289

Axiom
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated property: definition
annotated target: A type of spatial pattern of transcript expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC

subtractive hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: subtractive hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: subtractive hybridization evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of subtractive hybridization experiment that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T07:00:48Z
has_obo_namespace: eco
id: ECO:0000290

Axiom
annotated source: subtractive hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: subtractive hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: subtractive hybridization evidence used in manual assertion
annotated property: definition
annotated target: A type of subtractive hybridization experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC

transcript expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transcript expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression evidence
subclass of: expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of transcript expression evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2010-12-20T07:02:08Z
has_obo_namespace: eco
id: ECO:0000291

Axiom
annotated source: transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: transcript expression evidence used in manual assertion
annotated property: definition
annotated target: A type of transcript expression evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

morpholino experiment evidence
type: Class
subclass of: mutant phenotype evidence
definition: A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell.
created by: mchibucos
creation date: 2011-01-10T03:36:25Z
has_broad_synonym: anti-sense
has_obo_namespace: eco
id: ECO:0000292
comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method.

Axiom
annotated source: morpholino experiment evidence
annotated property: definition
annotated target: A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell.
database_cross_reference: ECO:MCC

systematic evolution of ligands by exponential amplification evidence
type: Class
subclass of: physical interaction evidence
definition: A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand.
created by: mchibucos
creation date: 2011-01-10T04:23:50Z
database_cross_reference: PSI-MI:MI:0657
has_exact_synonym: SELEX evidence
has_exact_synonym: in vitro selection evidence
has_obo_namespace: eco
has_related_synonym: in vitro evolution evidence
id: ECO:0000293
comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein.

Axiom
annotated source: systematic evolution of ligands by exponential amplification evidence
annotated property: definition
annotated target: A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand.
database_cross_reference: ECO:MCC
database_cross_reference: url:http://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment

systematic evolution of ligands by exponential enrichment
type: Axiom
annotated source: systematic evolution of ligands by exponential amplification evidence
annotated property: database_cross_reference
annotated target: PSI-MI:MI:0657

bacterial one-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
definition: A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey).
created by: mchibucos
creation date: 2011-01-10T06:16:20Z
has_exact_synonym: B1H evidence
has_obo_namespace: eco
id: ECO:0000294
comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes.

Axiom
annotated source: bacterial one-hybrid evidence
annotated property: definition
annotated target: A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey).
database_cross_reference: ECO:MCC

RNA-seq evidence
type: Class
subclass of: transcript expression evidence
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules.
created by: mchibucos
creation date: 2011-01-11T12:54:50Z
has_exact_synonym: WTSS
has_exact_synonym: whole transcriptome shotgun sequencing
has_obo_namespace: eco
has_related_synonym: RNA sequencing
id: ECO:0000295
comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.

Axiom
annotated source: RNA-seq evidence
annotated property: definition
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules.
database_cross_reference: ECO:MCC

Axiom
annotated source: RNA-seq evidence
annotated property: has_exact_synonym
annotated target: whole transcriptome shotgun sequencing
database_cross_reference: PMID:18611170

green fluorescent protein transcript localization evidence
type: Class
subclass of: transcriptional activation assay
definition: A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene.
created by: mchibucos
creation date: 2011-01-11T02:31:19Z
has_exact_synonym: localization of GFP transcript
has_obo_namespace: eco
id: ECO:0000296
comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of green fluorescent protein".

Axiom
annotated source: green fluorescent protein transcript localization evidence
annotated property: definition
annotated target: A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene.
database_cross_reference: ECO:MCC

LacZ transcript localization evidence
type: Class
subclass of: transcriptional activation assay
definition: A transcriptional activation assay that uses a LacZ reporter gene.
created by: mchibucos
creation date: 2011-01-11T02:53:19Z
has_obo_namespace: eco
id: ECO:0000297
comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of LacZ protein".

Axiom
annotated source: LacZ transcript localization evidence
annotated property: definition
annotated target: A transcriptional activation assay that uses a LacZ reporter gene.
database_cross_reference: ECO:MCC

cleavage arrested development evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked.
created by: mchibucos
creation date: 2011-01-11T03:28:49Z
has_obo_namespace: eco
id: ECO:0000298
comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest.

Axiom
annotated source: cleavage arrested development evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked.
database_cross_reference: ECO:MCC

cytochalasin experiment evidence
type: Class
subclass of: cleavage arrested development evidence
definition: A type of cleavage arrested development that arises after treatment with cytochalasin.
created by: mchibucos
creation date: 2011-01-11T03:30:29Z
has_obo_namespace: eco
id: ECO:0000299
comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments.

Axiom
annotated source: cytochalasin experiment evidence
annotated property: definition
annotated target: A type of cleavage arrested development that arises after treatment with cytochalasin.
database_cross_reference: ECO:MCC

green fluorescent protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
definition: A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker.
created by: mchibucos
creation date: 2011-01-11T03:48:50Z
has_exact_synonym: GFP immunolocalization evidence
has_obo_namespace: eco
id: ECO:0000300
comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript".

Axiom
annotated source: green fluorescent protein immunolocalization evidence
annotated property: definition
annotated target: A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker.
database_cross_reference: ECO:MCC

beta-galactosidase protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
definition: A type of immunolocalization evidence that was generated using LacZ protein as a marker.
created by: mchibucos
creation date: 2011-01-11T04:06:40Z
has_exact_synonym: LacZ protein immunolocalization evidence
has_obo_namespace: eco
id: ECO:0000301
comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript".

Axiom
annotated source: beta-galactosidase protein immunolocalization evidence
annotated property: definition
annotated target: A type of immunolocalization evidence that was generated using LacZ protein as a marker.
database_cross_reference: ECO:MCC

author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: author statement
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of author statement that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:26:47Z
has_obo_namespace: eco
id: ECO:0000302

Axiom
annotated source: author statement used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: author statement used in manual assertion
annotated property: definition
annotated target: A type of author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC

non-traceable author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: non-traceable author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: non-traceable author statement
subclass of: author statement used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of non-traceable author statement that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:29:50Z
database_cross_reference: GOECO:NAS
has_exact_synonym: NAS
has_exact_synonym: non-traceable author statement
has_obo_namespace: eco
id: ECO:0000303

Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: author statement used in manual assertion
is inferred: true

Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: definition
annotated target: A type of non-traceable author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC

non-traceable author statement
type: Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:NAS

Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: has_exact_synonym
annotated target: NAS
database_cross_reference: GOECO:NAS

Axiom
annotated source: non-traceable author statement used in manual assertion
annotated property: has_exact_synonym
annotated target: non-traceable author statement
database_cross_reference: GOECO:NAS

traceable author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: traceable author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: traceable author statement
subclass of: author statement used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of traceable author statement that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:31:03Z
database_cross_reference: GOECO:TAS
has_exact_synonym: TAS
has_exact_synonym: traceable author statement
has_obo_namespace: eco
id: ECO:0000304

Axiom
annotated source: traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: author statement used in manual assertion
is inferred: true

Axiom
annotated source: traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: traceable author statement used in manual assertion
annotated property: definition
annotated target: A type of traceable author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC

traceable author statement
type: Axiom
annotated source: traceable author statement used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:TAS

Axiom
annotated source: traceable author statement used in manual assertion
annotated property: has_exact_synonym
annotated target: TAS
database_cross_reference: GOECO:TAS

Axiom
annotated source: traceable author statement used in manual assertion
annotated property: has_exact_synonym
annotated target: traceable author statement
database_cross_reference: GOECO:TAS

curator inference used in manual assertion
type: Class
equivalent class: Class
  intersection of: curator inference
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: curator inference
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of curator inference that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:33:32Z
database_cross_reference: GOECO:IC
has_exact_synonym: IC
has_exact_synonym: inferred by curator
has_obo_namespace: eco
id: ECO:0000305
in_subset: valid with biological process
in_subset: valid with cellular component
in_subset: valid with molecular function

Axiom
annotated source: curator inference used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: curator inference used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: curator inference used in manual assertion
annotated property: definition
annotated target: A type of curator inference that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred by curator
type: Axiom
annotated source: curator inference used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IC

Axiom
annotated source: curator inference used in manual assertion
annotated property: has_exact_synonym
annotated target: IC
database_cross_reference: GOECO:IC

Axiom
annotated source: curator inference used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred by curator
database_cross_reference: GOECO:IC

inference from background scientific knowledge used in manual assertion
type: Class
equivalent class: Class
  intersection of: inference from background scientific knowledge
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: inference from background scientific knowledge
subclass of: curator inference used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of inference from background scientific knowledge that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:35:11Z
has_obo_namespace: eco
id: ECO:0000306

Axiom
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: curator inference used in manual assertion
is inferred: true

Axiom
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: definition
annotated target: A type of inference from background scientific knowledge that is used in a manual assertion.
database_cross_reference: ECO:MCC

no biological data found used in manual assertion
type: Class
equivalent class: Class
  intersection of: no biological data found
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: no biological data found
subclass of: curator inference used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of no biological data found that is used in a manual assertion.
created by: mchibucos
creation date: 2011-01-20T02:43:18Z
database_cross_reference: GOECO:ND
has_exact_synonym: ND
has_exact_synonym: no biological data available
has_obo_namespace: eco
id: ECO:0000307

Axiom
annotated source: no biological data found used in manual assertion
annotated property: subClassOf
annotated target: curator inference used in manual assertion
is inferred: true

Axiom
annotated source: no biological data found used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: no biological data found used in manual assertion
annotated property: definition
annotated target: A type of no biological data found that is used in a manual assertion.
database_cross_reference: ECO:MCC

no biological data available
type: Axiom
annotated source: no biological data found used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:ND

Axiom
annotated source: no biological data found used in manual assertion
annotated property: has_exact_synonym
annotated target: ND
database_cross_reference: GOECO:ND

Axiom
annotated source: no biological data found used in manual assertion
annotated property: has_exact_synonym
annotated target: no biological data available
database_cross_reference: GOECO:ND

biological aspect of ancestor evidence
type: Class
subclass of: phylogenetic evidence
definition: A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene.
created by: mchibucos
creation date: 2011-02-04T05:41:26Z
has_obo_namespace: eco
id: ECO:0000308
comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations.

Axiom
annotated source: biological aspect of ancestor evidence
annotated property: definition
annotated target: A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene.
database_cross_reference: ECO:MCC

cap analysis of gene expression evidence
type: Class
subclass of: transcript expression evidence
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system.
created by: mchibucos
creation date: 2011-02-17T01:33:09Z
has_exact_synonym: CAGE
has_obo_namespace: eco
id: ECO:0000309
comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.

Axiom
annotated source: cap analysis of gene expression evidence
annotated property: definition
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system.
database_cross_reference: ECO:MCC

Axiom
annotated source: cap analysis of gene expression evidence
annotated property: has_exact_synonym
annotated target: CAGE
database_cross_reference: PMID:16489339

nano-cap analysis of gene expression evidence
type: Class
subclass of: transcript expression evidence
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching.
created by: mchibucos
creation date: 2011-02-17T01:33:34Z
has_obo_namespace: eco
has_related_synonym: nanoCAGE
id: ECO:0000310
comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA.

Axiom
annotated source: nano-cap analysis of gene expression evidence
annotated property: definition
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching.
database_cross_reference: ECO:MCC

Axiom
annotated source: nano-cap analysis of gene expression evidence
annotated property: has_related_synonym
annotated target: nanoCAGE
database_cross_reference: PMID:20543846

imported information
type: Class
subclass of: evidence
definition: An evidence type that is based on work performed by a person or group prior to a use by different person or group.
created by: mchibucos
creation date: 2011-03-02T05:24:13Z
has_obo_namespace: eco
id: ECO:0000311

Axiom
annotated source: imported information
annotated property: definition
annotated target: An evidence type that is based on work performed by a person or group prior to a use by different person or group.
database_cross_reference: ECO:MCC

imported information used in manual assertion
type: Class
equivalent class: Class
  intersection of: imported information
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: imported information
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of imported information that is used in a manual assertion.
created by: mchibucos
creation date: 2011-03-02T05:29:44Z
has_obo_namespace: eco
id: ECO:0000312

Axiom
annotated source: imported information used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: imported information used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: imported information used in manual assertion
annotated property: definition
annotated target: A type of imported information that is used in a manual assertion.
database_cross_reference: ECO:MCC

imported information used in automatic assertion
type: Class
equivalent class: Class
  intersection of: imported information
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: imported information
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of imported information that is used in an automatic assertion.
created by: mchibucos
creation date: 2011-03-02T05:33:43Z
has_obo_namespace: eco
id: ECO:0000313

Axiom
annotated source: imported information used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: imported information used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: imported information used in automatic assertion
annotated property: definition
annotated target: A type of imported information that is used in an automatic assertion.
database_cross_reference: ECO:MCC

direct assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: direct assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of direct assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T04:59:31Z
database_cross_reference: GOECO:IDA
has_exact_synonym: inferred from direct assay
has_obo_namespace: eco
has_related_synonym: IDA
id: ECO:0000314
in_subset: valid with chemical entity

Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: definition
annotated target: A type of direct assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from direct assay
type: Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IDA

Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from direct assay
database_cross_reference: GOECO:IDA

Axiom
annotated source: direct assay evidence used in manual assertion
annotated property: has_related_synonym
annotated target: IDA
database_cross_reference: GOECO:IDA

mutant phenotype evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mutant phenotype evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: mutant phenotype evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of mutant phenotype evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:12:49Z
database_cross_reference: GOECO:IMP
has_obo_namespace: eco
has_related_synonym: IMP
has_related_synonym: inferred from mutant phenotype
id: ECO:0000315
in_subset: valid with chemical entity
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: definition
annotated target: A type of mutant phenotype evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from mutant phenotype
type: Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IMP

Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: has_related_synonym
annotated target: IMP
database_cross_reference: GOECO:IMP

Axiom
annotated source: mutant phenotype evidence used in manual assertion
annotated property: has_related_synonym
annotated target: inferred from mutant phenotype
database_cross_reference: GOECO:IMP

genetic interaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetic interaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genetic interaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genetic interaction experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:14:42Z
database_cross_reference: GOECO:IGI
has_exact_synonym: IGI
has_exact_synonym: inferred from genetic interaction
has_obo_namespace: eco
id: ECO:0000316
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: definition
annotated target: A type of genetic interaction experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from genetic interaction
type: Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IGI

Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IGI
database_cross_reference: GOECO:IGI

Axiom
annotated source: genetic interaction evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from genetic interaction
database_cross_reference: GOECO:IGI

genomic context evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genomic context evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genomic context evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genomic context evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:22:53Z
database_cross_reference: GOECO:IGC
has_exact_synonym: IGC
has_exact_synonym: inferred from genomic context
has_obo_namespace: eco
id: ECO:0000317
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: definition
annotated target: A type of genomic context evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from genomic context
type: Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IGC

Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IGC
database_cross_reference: GOECO:IGC

Axiom
annotated source: genomic context evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from genomic context
database_cross_reference: GOECO:IGC

biological aspect of ancestor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological aspect of ancestor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: similarity evidence used in manual assertion
subclass of: biological aspect of ancestor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological aspect of ancestor evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:23:51Z
database_cross_reference: GOECO:IBA
has_exact_synonym: IBA
has_exact_synonym: inferred from biological aspect of ancestor
has_obo_namespace: eco
id: ECO:0000318
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: definition
annotated target: A type of biological aspect of ancestor evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from biological aspect of ancestor
type: Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IBA

Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IBA
database_cross_reference: GOECO:IBA

Axiom
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from biological aspect of ancestor
database_cross_reference: GOECO:IBA

biological aspect of descendant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological aspect of descendant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biological aspect of descendant evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological aspect of descendant evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:25:09Z
database_cross_reference: GOECO:IBD
has_exact_synonym: IBD
has_exact_synonym: inferred from biological aspect of descendant
has_obo_namespace: eco
id: ECO:0000319
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: definition
annotated target: A type of biological aspect of descendant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from biological aspect of descendant
type: Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IBD

Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IBD
database_cross_reference: GOECO:IBD

Axiom
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from biological aspect of descendant
database_cross_reference: GOECO:IBD

phylogenetic determination of loss of key residues evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phylogenetic determination of loss of key residues evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: phylogenetic determination of loss of key residues evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:25:48Z
database_cross_reference: GOECO:IKR
database_cross_reference: GOECO:IMR
has_exact_synonym: IKR
has_exact_synonym: IMR
has_exact_synonym: inferred from key residues
has_exact_synonym: inferred from missing residues
has_obo_namespace: eco
id: ECO:0000320

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: definition
annotated target: A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from key residues
type: Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IKR

inferred from missing residues
type: Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IMR

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IKR
database_cross_reference: GOECO:IKR

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IMR
database_cross_reference: GOECO:IKR

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from key residues
database_cross_reference: GOECO:IKR

Axiom
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from missing residues
database_cross_reference: GOECO:IKR

rapid divergence from ancestral sequence evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: rapid divergence from ancestral sequence evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: rapid divergence from ancestral sequence evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2011-10-28T05:26:42Z
database_cross_reference: GOECO:IRD
has_exact_synonym: IRD
has_exact_synonym: inferred from rapid divergence
has_obo_namespace: eco
id: ECO:0000321
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: subClassOf
annotated target: similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: definition
annotated target: A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from rapid divergence
type: Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IRD

Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IRD
database_cross_reference: GOECO:IRD

Axiom
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from rapid divergence
database_cross_reference: GOECO:IRD

imported manually asserted information used in automatic assertion
type: Class
subclass of: imported information used in automatic assertion
definition: Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
created by: mchibucos
creation date: 2011-12-13T12:04:48Z
database_cross_reference: GO_REF:0000037
database_cross_reference: GO_REF:0000039
database_cross_reference: GO_REF:0000041
has_obo_namespace: eco
id: ECO:0000322

Axiom
annotated source: imported manually asserted information used in automatic assertion
annotated property: definition
annotated target: Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
database_cross_reference: ECO:MCC

Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries.
type: Axiom
annotated source: imported manually asserted information used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000037

Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries.
type: Axiom
annotated source: imported manually asserted information used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000039

IEA (UniProt UniPathway)
type: Axiom
annotated source: imported manually asserted information used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000041

imported automatically asserted information used in automatic assertion
type: Class
subclass of: imported information used in automatic assertion
definition: A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
created by: mchibucos
creation date: 2011-12-13T02:06:25Z
database_cross_reference: GO_REF:0000038
database_cross_reference: GO_REF:0000040
has_obo_namespace: eco
id: ECO:0000323

Axiom
annotated source: imported automatically asserted information used in automatic assertion
annotated property: definition
annotated target: A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
database_cross_reference: ECO:MCC

Gene Ontology annotation based on automatic assignment of UniProtKB keywords in UniProtKB/TrEMBL entries.
type: Axiom
annotated source: imported automatically asserted information used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000038

Gene Ontology annotation based on the automatic assignment of UniProtKB Subcellular Location terms in UniProtKB/TrEMBL entries.
type: Axiom
annotated source: imported automatically asserted information used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000040

imaging assay evidence
type: Class
subclass of: direct assay evidence
definition: Direct assay evidence derived from analysis of an image.
created by: mchibucos
creation date: 2012-02-23T12:01:43Z
has_alternative_id: ECO:0001122
has_narrow_synonym: radiologic test evidence
has_obo_namespace: eco
id: ECO:0000324

Axiom
annotated source: imaging assay evidence
annotated property: definition
annotated target: Direct assay evidence derived from analysis of an image.
database_cross_reference: ECO:MCC

chromatography evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase.
created by: mchibucos
creation date: 2012-05-16T11:46:27Z
has_exact_synonym: chromatographic evidence
has_obo_namespace: eco
id: ECO:0000325

Axiom
annotated source: chromatography evidence
annotated property: definition
annotated target: A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase.
database_cross_reference: ECO:MCC

transcript splice pattern evidence
type: Class
subclass of: sequence alignment evidence
definition: A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript.
created by: mchibucos
creation date: 2012-06-08T11:03:59Z
has_obo_namespace: eco
id: ECO:0000326

Axiom
annotated source: transcript splice pattern evidence
annotated property: definition
annotated target: A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript.
database_cross_reference: ECO:MCC

whole transcript splice pattern evidence
type: Class
subclass of: transcript splice pattern evidence
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features.
created by: mchibucos
creation date: 2012-06-08T11:11:24Z
has_obo_namespace: eco
id: ECO:0000327
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.

Axiom
annotated source: whole transcript splice pattern evidence
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features.
database_cross_reference: ECO:MCC

coding sequence splice pattern evidence
type: Class
subclass of: transcript splice pattern evidence
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript.
created by: mchibucos
creation date: 2012-06-08T11:21:57Z
has_obo_namespace: eco
id: ECO:0000328
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.

Axiom
annotated source: coding sequence splice pattern evidence
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript.
database_cross_reference: ECO:MCC

whole transcript splice pattern evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: whole transcript splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: whole transcript splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion.
created by: mchibucos
creation date: 2012-06-08T11:40:03Z
has_obo_namespace: eco
id: ECO:0000329
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.

Axiom
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion.
database_cross_reference: ECO:MCC

coding sequence splice pattern evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: coding sequence splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: coding sequence splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion.
created by: mchibucos
creation date: 2012-06-08T11:43:47Z
has_obo_namespace: eco
id: ECO:0000330
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.

Axiom
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion.
database_cross_reference: ECO:MCC

coding sequence splice pattern evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: coding sequence splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: coding sequence splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion.
created by: mchibucos
creation date: 2012-06-08T11:48:09Z
has_obo_namespace: eco
id: ECO:0000331
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.

Axiom
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion.
database_cross_reference: ECO:MCC

whole transcript splice pattern evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: whole transcript splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: whole transcript splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion.
created by: mchibucos
creation date: 2012-06-08T11:49:17Z
has_obo_namespace: eco
id: ECO:0000332
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.

Axiom
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: definition
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion.
database_cross_reference: ECO:MCC

sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
definition: A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility.
created by: mchibucos
creation date: 2012-07-31T02:56:43Z
has_exact_synonym: SDS-PAGE evidence
has_obo_namespace: eco
id: ECO:0000333
comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size.

Axiom
annotated source: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
annotated property: definition
annotated target: A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility.
database_cross_reference: ECO:MCC
database_cross_reference: PubMed:4861258
database_cross_reference: url:http://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html

particle size and count assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined.
created by: mchibucos
creation date: 2012-07-31T03:19:32Z
has_obo_namespace: eco
id: ECO:0000334
comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system.

Axiom
annotated source: particle size and count assay evidence
annotated property: definition
annotated target: A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined.
database_cross_reference: ECO:MCC

substance quantification evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where the quantity of a substance used as part of an assay is measured.
created by: mchibucos
creation date: 2012-07-31T03:52:46Z
has_obo_namespace: eco
id: ECO:0000335
comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme.

Axiom
annotated source: substance quantification evidence
annotated property: definition
annotated target: A type of direct assay evidence where the quantity of a substance used as part of an assay is measured.
database_cross_reference: ECO:MCC

competitive growth assay evidence
type: Class
subclass of: biological assay evidence
definition: A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed.
created by: mchibucos
creation date: 2012-08-24T12:03:58Z
has_exact_synonym: fitness profiling
has_obo_namespace: eco
id: ECO:0000336

Axiom
annotated source: competitive growth assay evidence
annotated property: definition
annotated target: A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed.
database_cross_reference: GOC:MAH
database_cross_reference: PMID:14718172
database_cross_reference: PMID:20537132

Axiom
annotated source: competitive growth assay evidence
annotated property: has_exact_synonym
annotated target: fitness profiling
database_cross_reference: GOC:MAH
database_cross_reference: PMID:20537132

gel electrophoresis evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field.
created by: mchibucos
creation date: 2012-08-24T12:11:51Z
has_obo_namespace: eco
id: ECO:0000337
comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch.

Axiom
annotated source: gel electrophoresis evidence
annotated property: definition
annotated target: A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field.
database_cross_reference: ECO:MCC
database_cross_reference: wikipedia:Gel_electrophoresis

pulsed-field gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
definition: Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel.
created by: mchibucos
creation date: 2012-08-24T12:34:01Z
has_exact_synonym: PFGE
has_obo_namespace: eco
id: ECO:0000338

Axiom
annotated source: pulsed-field gel electrophoresis evidence
annotated property: definition
annotated target: Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel.
database_cross_reference: PMID:6373014

two-dimensional agarose gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
definition: A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide.
created by: mchibucos
creation date: 2012-08-24T12:42:37Z
has_exact_synonym: 2-D agarose gel electrophoresis
has_obo_namespace: eco
id: ECO:0000339
comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape.

Axiom
annotated source: two-dimensional agarose gel electrophoresis evidence
annotated property: definition
annotated target: A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide.
database_cross_reference: PMID:16118435
database_cross_reference: PMID:8594382

plasmid maintenance assay evidence
type: Class
subclass of: experimental evidence
definition: Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated.
created by: mchibucos
creation date: 2012-08-24T01:27:58Z
has_exact_synonym: minichromosome maintenance assay evidence
has_obo_namespace: eco
id: ECO:0000340

Axiom
annotated source: plasmid maintenance assay evidence
annotated property: definition
annotated target: Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated.
database_cross_reference: ECO:MCC
database_cross_reference: PMID:6323245

Axiom
annotated source: plasmid maintenance assay evidence
annotated property: has_exact_synonym
annotated target: minichromosome maintenance assay evidence
database_cross_reference: PMID:6323245

specific protein inhibition by antibody evidence
type: Class
subclass of: specific protein inhibition evidence
definition: A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody.
created by: mchibucos
creation date: 2012-08-24T01:48:24Z
has_obo_namespace: eco
id: ECO:0000341

Axiom
annotated source: specific protein inhibition by antibody evidence
annotated property: definition
annotated target: A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody.
database_cross_reference: ECO:MCC

Support of intron position by RNA-seq alignment evidence
type: Class
subclass of: RNA-seq evidence
definition: A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.
created by: mchibucos
creation date: 2013-04-03T04:22:05Z
has_obo_namespace: eco
id: ECO:0000342
comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data.

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence
annotated property: definition
annotated target: A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.
database_cross_reference: ECO:MCC

Full support of intron position by RNA-seq alignment evidence
type: Class
subclass of: Support of intron position by RNA-seq alignment evidence
definition: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript.
created by: mchibucos
creation date: 2013-04-03T04:41:48Z
has_obo_namespace: eco
id: ECO:0000343
comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not.

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence
annotated property: definition
annotated target: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript.
database_cross_reference: ECO:MCC

Partial support of intron position by RNA-seq alignment evidence
type: Class
subclass of: Support of intron position by RNA-seq alignment evidence
definition: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript.
created by: mchibucos
creation date: 2013-04-03T04:50:46Z
has_obo_namespace: eco
id: ECO:0000344
comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample.

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence
annotated property: definition
annotated target: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript.
database_cross_reference: ECO:MCC

single exon transcript confirmation via alignment evidence
type: Class
subclass of: sequence alignment evidence
created by: mchibucos
creation date: 2013-04-03T05:10:34Z
has_obo_namespace: eco
id: ECO:0000345

Support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression evidence used in manual assertion
subclass of: Support of intron position by RNA-seq alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2013-04-04T04:37:35Z
has_obo_namespace: eco
id: ECO:0000346

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition
annotated target: A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Support of intron position by RNA-seq alignment evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2013-04-04T04:38:45Z
has_obo_namespace: eco
id: ECO:0000347

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition
annotated target: A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

Full support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Full support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Full support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2013-04-04T04:40:19Z
has_obo_namespace: eco
id: ECO:0000348

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Support of intron position by RNA-seq alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition
annotated target: A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

Full support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Full support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Full support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2013-04-04T04:41:34Z
has_obo_namespace: eco
id: ECO:0000349

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Support of intron position by RNA-seq alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition
annotated target: A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Partial support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Partial support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
created by: mchibucos
creation date: 2013-04-04T04:42:53Z
has_obo_namespace: eco
id: ECO:0000350

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Support of intron position by RNA-seq alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition
annotated target: A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC

Partial support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Partial support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Partial support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2013-04-04T04:43:56Z
has_obo_namespace: eco
id: ECO:0000351

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Support of intron position by RNA-seq alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition
annotated target: A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence that is used in an manual assertion.
created by: mchibucos
has_obo_namespace: eco
id: ECO:0000352

Axiom
annotated source: evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence
is inferred: true

Axiom
annotated source: evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence that is used in an manual assertion.
database_cross_reference: ECO:MCC

physical interaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: physical interaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of physical interaction evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2013-10-31T21:01:27Z
database_cross_reference: GOECO:IPI
has_exact_synonym: IPI
has_exact_synonym: inferred from physical interaction
has_obo_namespace: eco
id: ECO:0000353
in_subset: valid with chemical entity
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: definition
annotated target: A type of physical interaction evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

inferred from physical interaction
type: Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GOECO:IPI

Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: IPI
database_cross_reference: GOECO:IPI

Axiom
annotated source: physical interaction evidence used in manual assertion
annotated property: has_exact_synonym
annotated target: inferred from physical interaction
database_cross_reference: GOECO:IPI

gene neighbors evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: gene neighbors evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: gene neighbors evidence
subclass of: genomic context evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of gene neighbors evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2013-11-19T13:37:24Z
database_cross_reference: GO_REF:0000025
has_obo_namespace: eco
id: ECO:0000354

Axiom
annotated source: gene neighbors evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: gene neighbors evidence used in manual assertion
annotated property: definition
annotated target: A type of gene neighbors evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

operon structure as IGC evidence
type: Axiom
annotated source: gene neighbors evidence used in manual assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000025

phylogenetic distribution evidence
type: Class
subclass of: phylogenetic evidence
definition: A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony.
created by: mchibucos
creation date: 2014-04-22T17:02:31Z
has_obo_namespace: eco
id: ECO:0000355
comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci.

Axiom
annotated source: phylogenetic distribution evidence
annotated property: definition
annotated target: A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony.
database_cross_reference: ECO:MCC
database_cross_reference: PMID:21238344

differential gene expression evidence from microarray experiment
type: Class
subclass of: transcript expression evidence
created by: mchibucos
creation date: 2014-09-26T16:21:45Z
has_obo_namespace: eco
id: ECO:0000356

differential gene expression evidence from RNA-seq experiment
type: Class
subclass of: transcript expression evidence
created by: mchibucos
creation date: 2014-09-26T16:22:57Z
has_obo_namespace: eco
id: ECO:0000357

differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact)
type: Class
subclass of: transcript expression evidence
created by: mchibucos
creation date: 2014-09-26T16:25:47Z
has_obo_namespace: eco
id: ECO:0000358

differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact)
type: Class
subclass of: transcript expression evidence
created by: mchibucos
creation date: 2014-09-26T16:26:48Z
has_obo_namespace: eco
id: ECO:0000359

biological target-disease association via drug
type: Class
subclass of: experimental evidence
created by: mchibucos
creation date: 2014-09-26T16:39:11Z
has_obo_namespace: eco
id: ECO:0000360

inferential evidence
type: Class
subclass of: evidence
definition: A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means.
created by: mchibucos
creation date: 2015-07-14T11:19:37Z
has_obo_namespace: eco
id: ECO:0000361
comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true.

Axiom
annotated source: inferential evidence
annotated property: definition
annotated target: A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means.
database_cross_reference: ECO:MCC

evidence based on computational logical inference
type: Class
subclass of: inferential evidence
definition: A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is computationally derived.
created by: mchibucos
creation date: 2015-07-14T11:24:27Z
has_obo_namespace: eco
id: ECO:0000362

Axiom
annotated source: evidence based on computational logical inference
annotated property: definition
annotated target: A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is computationally derived.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:MC

evidence based on computational logical inference used in automatic assertion
type: Class
equivalent class: Class
  intersection of: evidence based on computational logical inference
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence based on computational logical inference
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of evidence based on computational logical inference that is used in automatic assertion.
created by: mchibucos
creation date: 2015-07-14T11:35:08Z
database_cross_reference: GO_REF:0000108
has_obo_namespace: eco
id: ECO:0000363

Axiom
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: definition
annotated target: A type of evidence based on computational logical inference that is used in automatic assertion.
database_cross_reference: ECO:MCC

Automatic assignment of GO terms using logical inference, based on on inter-ontology links
type: Axiom
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000108

evidence based on logical inference from manual annotation used in automatic assertion
type: Class
subclass of: evidence based on computational logical inference used in automatic assertion
definition: A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion.
created by: mchibucos
creation date: 2015-07-14T11:44:19Z
has_obo_namespace: eco
id: ECO:0000364

Axiom
annotated source: evidence based on logical inference from manual annotation used in automatic assertion
annotated property: definition
annotated target: A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion.
database_cross_reference: ECO:MCC

evidence based on logical inference from automatic annotation used in automatic assertion
type: Class
subclass of: evidence based on computational logical inference used in automatic assertion
definition: A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion.
created by: mchibucos
creation date: 2015-07-14T11:45:25Z
has_obo_namespace: eco
id: ECO:0000366

Axiom
annotated source: evidence based on logical inference from automatic annotation used in automatic assertion
annotated property: definition
annotated target: A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion.
database_cross_reference: ECO:MCC

evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of evidence that is used in an automatic assertion.
created by: cjm
database_cross_reference: GOECO:IEA
database_cross_reference: GO_REF:0000003
database_cross_reference: GO_REF:0000004
database_cross_reference: GO_REF:0000023
has_exact_synonym: IEA
has_exact_synonym: inferred from electronic annotation
has_obo_namespace: eco
id: ECO:0000501
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
annotated source: evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence
is inferred: true

Axiom
annotated source: evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: evidence used in automatic assertion
annotated property: definition
annotated target: A type of evidence that is used in an automatic assertion.
database_cross_reference: ECO:cjm

inferred from electronic annotation
type: Axiom
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GOECO:IEA

Gene Ontology annotation based on Enzyme Commission mapping.
type: Axiom
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000003

Gene Ontology annotation based on Swiss-Prot keyword mapping.
type: Axiom
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000004

Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
type: Axiom
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference
annotated target: GO_REF:0000023

Axiom
annotated source: evidence used in automatic assertion
annotated property: has_exact_synonym
annotated target: IEA
database_cross_reference: GOECO:IEA

Axiom
annotated source: evidence used in automatic assertion
annotated property: has_exact_synonym
annotated target: inferred from electronic annotation
database_cross_reference: GOECO:IEA

3D cell culture evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001001

[3H]arachidonic acid release assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001002

[3H]-thymidine incorporation assay evidence
type: Class
subclass of: DNA synthesis cell proliferation assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001003

51Cr release assay evidence
type: Class
subclass of: cytotoxicity assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001004

7-aminoactinomycin staining evidence
type: Class
subclass of: staining evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: 7-AAD staining evidence
has_exact_synonym: 7-amino-actinomycin D staining evidence
has_obo_namespace: eco
id: ECO:0001005

adhesion assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001006

adoptive cell transfer evidence
type: Class
subclass of: ex vivo assay evidence
definition: A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: Adoptive immunotherapy
has_exact_synonym: passive immunization
has_obo_namespace: eco
id: ECO:0001007

Axiom
annotated source: adoptive cell transfer evidence
annotated property: definition
annotated target: A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.
database_cross_reference: ECO:SN
database_cross_reference: url:http://jco.ascopubs.org/content/23/10/2346.full.pdf+html
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf

Alamar blue assay evidence
type: Class
subclass of: cell viability assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001008

allograft transplantation experiment evidence
type: Class
subclass of: anatomical perturbation evidence
definition: A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: Allotransplantation
has_exact_synonym: allografting
has_narrow_synonym: tissue grafting
has_obo_namespace: eco
id: ECO:0001009

Axiom
annotated source: allograft transplantation experiment evidence
annotated property: definition
annotated target: A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/subjects/allograft
database_cross_reference: url:http://www.organdonor.gov/about/terms_and_topics/

anion-exchange chromatography evidence
type: Class
subclass of: chromatography evidence
definition: A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001010

Axiom
annotated source: anion-exchange chromatography evidence
annotated property: definition
annotated target: A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules.
database_cross_reference: ECO:SN
database_cross_reference: PMID:20978968
database_cross_reference: url:http://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography

annexin-V staining evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001011

behavioral assay evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001012

blocking monoclonal antibody evidence
type: Class
subclass of: immunological assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001013

blocking peptide evidence
type: Class
subclass of: immunological assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001014

blocking polyclonal antibody evidence
type: Class
subclass of: immunological assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001015

blood test evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001016

Axiom
annotated source: blood test evidence
annotated property: definition
annotated target: A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components.
database_cross_reference: ECO:SN
database_cross_reference: url:https://www.nhlbi.nih.gov/health/health-topics/topics/bdt

Boyden chamber assay evidence
type: Class
subclass of: chemotaxis assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001017

bromodeoxyuridine incorporation assay evidence
type: Class
subclass of: nucleotide analog incorporation evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: 5-bromo-2'-deoxyuridine incorporation assay evidence
has_exact_synonym: BUdR incorporation assay evidence
has_exact_synonym: BrdU incorporation assay evidence
has_exact_synonym: BrdUrd incorporation assay evidence
has_obo_namespace: eco
id: ECO:0001018

caspase assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001019

cell counting evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001020

cell permeability assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001021

carboxyfluorescein diacetate succinimidyl ester staining evidence
type: Class
subclass of: staining evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: CFDA-SE staining evidence
has_exact_synonym: CFSE-staining evidence
has_obo_namespace: eco
id: ECO:0001022

chemiluminescence-linked immunoassay evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: CLIA evidence
has_obo_namespace: eco
id: ECO:0001023

chimeric protein evidence
type: Class
subclass of: mutant phenotype evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001024

co-electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001025

co-localization evidence
type: Class
subclass of: imaging assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001026

colony counting evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001027

co-sedimentation assay evidence
type: Class
subclass of: physical interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001028

comet assay evidence
type: Class
subclass of: gel electrophoresis evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001029

conditional knockout evidence
type: Class
subclass of: knockout evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001030

conditional knockin evidence
type: Class
subclass of: knockin evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001031

constitutively active mutant evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001032

cross-linking evidence
type: Class
subclass of: protein binding evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001033

crystallography evidence
type: Class
subclass of: structure determination evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001034

cytochemistry evidence
type: Class
subclass of: expression pattern evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001035

cytochrome C release assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001036

4',6-diamidino-2-phenylindole staining evidence
type: Class
subclass of: staining evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: DAPI staining evidence
has_obo_namespace: eco
id: ECO:0001037

deletion evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001038

DNA laddering assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001039

RNA dot blot assay evidence
type: Class
subclass of: RNA detection assay evidence
definition: A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
has_related_synonym: RNA dot blot
id: ECO:0001040

Axiom
annotated source: RNA dot blot assay evidence
annotated property: definition
annotated target: A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21424648

RNA microarray evidence
type: Class
subclass of: RNA detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001041

dominant-negative mutant evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001042

Edman degradation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Edman degradation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: Edman degradation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Edman degradation evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:59:00Z
has_obo_namespace: eco
id: ECO:0001043

Axiom
annotated source: Edman degradation evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: Edman degradation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Edman degradation evidence used in manual assertion
annotated property: definition
annotated target: A type of Edman degradation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Edman degradation evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001044

eTag assay evidence
type: Class
subclass of: affinity evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001045

filter binding assay evidence
type: Class
subclass of: physical interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001046

fluorescence in situ hybridization evidence
type: Class
subclass of: expression pattern evidence
subclass of: in situ hybridization evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001047

fluorescence resonance energy transfer evidence
type: Class
subclass of: physical interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: FRET
has_obo_namespace: eco
id: ECO:0001048

gel-filtration evidence
type: Class
subclass of: chromatography evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001049

histochemistry evidence
type: Class
subclass of: expression pattern evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001050

histology evidence
type: Class
subclass of: imaging assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001051

high-performance liquid chromatography evidence
type: Class
subclass of: chromatography evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: HPLC evidence
has_obo_namespace: eco
id: ECO:0001052

immunocytochemistry evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001053

immunodepletion evidence
type: Class
subclass of: immunological assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001054

immunohistochemistry evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001055

induced mutation evidence
type: Class
subclass of: genetic transformation evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001056

in vitro acetylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001057

Axiom
annotated source: in vitro acetylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml

in vitro cleavage assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001058

Axiom
annotated source: in vitro cleavage assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments.
database_cross_reference: ECO:SN
database_cross_reference: PMID:21121091
database_cross_reference: SIB:PG

in vitro deubiquitination assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001059

Axiom
annotated source: in vitro deubiquitination assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG

in vitro deacetylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001060

Axiom
annotated source: in vitro deacetylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml

in vitro defarnesylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001061

Axiom
annotated source: in vitro defarnesylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:16126733
database_cross_reference: SIB:PG

in vitro demethylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001062

Axiom
annotated source: in vitro demethylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Demethylation

in vitro desumoylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001063

Axiom
annotated source: in vitro desumoylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/

in vitro farnesylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001064

Axiom
annotated source: in vitro farnesylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Farnesyltransferase

in vitro methylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001065

Axiom
annotated source: in vitro methylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Methylation

in vitro palmitoylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001066

Axiom
annotated source: in vitro palmitoylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17077383
database_cross_reference: SIB:PG

in vitro phosphatase assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001067

Axiom
annotated source: in vitro phosphatase assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Phosphatase

in vitro protein kinase assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001068

Axiom
annotated source: in vitro protein kinase assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_kinase
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_phosphorylation

in vitro polyADP-ribosylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001069

Axiom
annotated source: in vitro polyADP-ribosylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:21870253
database_cross_reference: SIB:PG

in vitro sumoylation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001070

Axiom
annotated source: in vitro sumoylation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/

in vitro transcription assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001071

Axiom
annotated source: in vitro transcription assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW
database_cross_reference: PMID:21125481
database_cross_reference: SIB:PG

in vitro translation assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001072

Axiom
annotated source: in vitro translation assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:18230759
database_cross_reference: SIB:PG

in vitro ubiquitination assay evidence
type: Class
subclass of: in vitro assay evidence
definition: A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001073

Axiom
annotated source: in vitro ubiquitination assay evidence
annotated property: definition
annotated target: A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG

in vivo acetylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001074

Axiom
annotated source: in vivo acetylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml

in vivo cleavage assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001075

Axiom
annotated source: in vivo cleavage assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22154596
database_cross_reference: SIB:PG

in vivo deacetylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001076

Axiom
annotated source: in vivo deacetylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml

in vivo defarnesylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001077

Axiom
annotated source: in vivo defarnesylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15556768
database_cross_reference: PMID:16507103
database_cross_reference: SIB:PG

in vivo demethylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001078

Axiom
annotated source: in vivo demethylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Demethylation

in vivo deubiquitination assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001079

Axiom
annotated source: in vivo deubiquitination assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG

in vivo desumoylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001080

Axiom
annotated source: in vivo desumoylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/

in vivo farnesylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001081

Axiom
annotated source: in vivo farnesylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:9030603
database_cross_reference: SIB:PG

in vivo methylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001082

Axiom
annotated source: in vivo methylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Methylation

in vivo palmitoylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001083

Axiom
annotated source: in vivo palmitoylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:10329400
database_cross_reference: SIB:PG

in vivo phosphatase assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001084

Axiom
annotated source: in vivo phosphatase assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Phosphatase

in vivo protein kinase assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001085

Axiom
annotated source: in vivo protein kinase assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_kinase
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_phosphorylation

in vivo sumoylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001086

Axiom
annotated source: in vivo sumoylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/

in vivo transcription assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where transcription is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001087

Axiom
annotated source: in vivo transcription assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where transcription is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:12181418
database_cross_reference: SIB:PG

in vivo translation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001088

Axiom
annotated source: in vivo translation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:24901308
database_cross_reference: SIB:PG

in vivo ubiquitination assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001089

Axiom
annotated source: in vivo ubiquitination assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG

knockin evidence
type: Class
subclass of: genetic transformation evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001090

knockout evidence
type: Class
subclass of: genetic transformation evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001091

lipid binding assay evidence
type: Class
subclass of: physical interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001092

luminescence-based mammalian interactome mapping assay evidence
type: Class
subclass of: physical interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: LUMIER assay
has_obo_namespace: eco
id: ECO:0001093

macroscopy evidence
type: Class
subclass of: experimental evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001094

mammalian 2-hybrid assay evidence
type: Class
subclass of: hybrid interaction evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001095

mass spectrometry evidence
type: Class
subclass of: spectrometry evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001096

medical imaging evidence
type: Class
subclass of: imaging assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001097

microscopy evidence
type: Class
subclass of: imaging assay evidence
definition: A type of direct assay evidence where a microscope was used to view a sample.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001098

Axiom
annotated source: microscopy evidence
annotated property: definition
annotated target: A type of direct assay evidence where a microscope was used to view a sample.
database_cross_reference: ECO:MCC

motility wound healing assay evidence
type: Class
subclass of: in vitro assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001099

MTS assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
has_obo_namespace: eco
id: ECO:0001100

MTT assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
has_obo_namespace: eco
id: ECO:0001101

multiplex bead-based immunoassay evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001102

natural variation mutant evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001103

nuclear fragmentation evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001104

nuclear magnetic resonance spectroscopy evidence
type: Class
subclass of: structure determination evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: NMR spectroscopy
has_obo_namespace: eco
id: ECO:0001105

nuclease protection assay evidence
type: Class
subclass of: RNA detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001106

nucleotide analog incorporation evidence
type: Class
subclass of: DNA synthesis cell proliferation assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001107

phage display evidence
type: Class
subclass of: protein binding evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001108

phosphoamino acid analysis evidence
type: Class
subclass of: protein assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001109

peptide affinity enrichment evidence
type: Class
subclass of: affinity evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: phosphopeptide affinity enrichment evidence
has_obo_namespace: eco
id: ECO:0001110

physical examination evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001111

peptide array evidence
type: Class
subclass of: protein assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: phosphopeptide array evidence
has_obo_namespace: eco
id: ECO:0001112

point mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001113

propidium iodide staining evidence
type: Class
subclass of: staining evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001114

fluorescence evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001115

protein dot blot assay evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001116

protein microarray evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001117

protein sequencing assay evidence
type: Class
subclass of: sequencing assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001118

quantitative mass spectrometry evidence
type: Class
subclass of: mass spectrometry evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001119

radioisotope assay evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: radioassay
has_exact_synonym: radioisotopic assay
has_obo_namespace: eco
id: ECO:0001120

radioimmunoassay evidence
type: Class
subclass of: protein detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001121

resonant mirror biosensor evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001123

restriction fragment detection evidence
type: Class
subclass of: DNA detection assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001124

spectrophotometry evidence
type: Class
subclass of: spectrometry evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001126

surface plasmon resonance evidence
type: Class
subclass of: physical interaction evidence
definition: A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: SPR evidence
has_obo_namespace: eco
id: ECO:0001127
comment: Plasmons are generated by an incident light beam.

Axiom
annotated source: surface plasmon resonance evidence
annotated property: definition
annotated target: A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11578932
database_cross_reference: PMID:19151937
database_cross_reference: PMID:8574707

Axiom
annotated source: surface plasmon resonance evidence
annotated property: has_exact_synonym
annotated target: SPR evidence
database_cross_reference: PMID:11578932

syngeneic transplantation experiment evidence
type: Class
subclass of: experimental phenotypic evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001128

TACE activity assay evidence
type: Class
subclass of: enzyme assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: Tumor necrosis factor - alpha converting enzyme activity assay
has_obo_namespace: eco
id: ECO:0001129

tissue microarray evidence
type: Class
subclass of: array experiment evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001130

transgenic organism evidence
type: Class
subclass of: genetic transformation evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001131

tryptic phosphopeptide mapping assay evidence
type: Class
subclass of: mass spectrometry evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001132

terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
type: Class
subclass of: apoptotic assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: TUNEL assay evidence
has_exact_synonym: TdT-mediated dUTP-biotin nick end labeling assay
has_exact_synonym: terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence
has_obo_namespace: eco
id: ECO:0001133

urine test evidence
type: Class
subclass of: direct assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001134

wound healing assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001135

WST-1 assay evidence
type: Class
subclass of: cell proliferation assay evidence
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_obo_namespace: eco
id: ECO:0001136

xenotransplantation experiment evidence
type: Class
subclass of: anatomical perturbation evidence
definition: A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient.
created by: mchibucos
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: Xenograft transplantation
has_exact_synonym: Xenografting
has_exact_synonym: Xenotransplantation
has_narrow_synonym: tissue grafting
has_obo_namespace: eco
id: ECO:0001137

Axiom
annotated source: xenotransplantation experiment evidence
annotated property: definition
annotated target: A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient.
database_cross_reference: ECO:SN
database_cross_reference: url:http://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html
database_cross_reference: url:http://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/

3D cell culture evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 3D cell culture evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 3D cell culture evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of 3D cell culture evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:18:35Z
has_obo_namespace: eco
id: ECO:0001138

Axiom
annotated source: 3D cell culture evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: 3D cell culture evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: 3D cell culture evidence used in manual assertion
annotated property: definition
annotated target: A type of 3D cell culture evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

51Cr release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 51Cr release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 51Cr release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of 51Cr release assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:20:55Z
has_obo_namespace: eco
id: ECO:0001139

Axiom
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: definition
annotated target: A type of 51Cr release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

7-aminoactinomycin staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 7-aminoactinomycin staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 7-aminoactinomycin staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of 7-aminoactinomycin staining evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:22:01Z
has_obo_namespace: eco
id: ECO:0001140

Axiom
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: definition
annotated target: A type of 7-aminoactinomycin staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

[3H]-thymidine incorporation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: [3H]-thymidine incorporation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: [3H]-thymidine incorporation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:31:52Z
has_obo_namespace: eco
id: ECO:0001141

Axiom
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

[3H]arachidonic acid release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: [3H]arachidonic acid release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: [3H]arachidonic acid release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:32:44Z
has_obo_namespace: eco
id: ECO:0001142

Axiom
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: definition
annotated target: A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

adhesion assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: adhesion assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: adhesion assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of adhesion assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:33:38Z
has_obo_namespace: eco
id: ECO:0001143

Axiom
annotated source: adhesion assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: adhesion assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: adhesion assay evidence used in manual assertion
annotated property: definition
annotated target: A type of adhesion assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

adoptive cell transfer evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: adoptive cell transfer evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: adoptive cell transfer evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of adoptive cell transfer evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:36:19Z
has_obo_namespace: eco
id: ECO:0001144

Axiom
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: definition
annotated target: A type of adoptive cell transfer evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Alamar blue assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Alamar blue assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Alamar blue assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Alamar blue assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:37:21Z
has_obo_namespace: eco
id: ECO:0001145

Axiom
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: definition
annotated target: A type of Alamar blue assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

allograft transplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: allograft transplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: allograft transplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of allograft transplantation experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:38:22Z
has_obo_namespace: eco
id: ECO:0001146

Axiom
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of allograft transplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

anion-exchange chromatography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: anion-exchange chromatography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: anion-exchange chromatography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of anion-exchange chromatography evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:44:30Z
has_obo_namespace: eco
id: ECO:0001147

Axiom
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: definition
annotated target: A type of anion-exchange chromatography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Axiom
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

annexin-V staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: annexin-V staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: annexin-V staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of annexin-V staining evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:52:01Z
has_obo_namespace: eco
id: ECO:0001148

Axiom
annotated source: annexin-V staining evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: annexin-V staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: annexin-V staining evidence used in manual assertion
annotated property: definition
annotated target: A type of annexin-V staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

behavioral assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: behavioral assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: behavioral assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of behavioral assay evidence used that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:53:38Z
has_obo_namespace: eco
id: ECO:0001149

Axiom
annotated source: behavioral assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: behavioral assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: behavioral assay evidence used in manual assertion
annotated property: definition
annotated target: A type of behavioral assay evidence used that is used in a manual assertion.
database_cross_reference: ECO:MCC

blocking monoclonal antibody evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking monoclonal antibody evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking monoclonal antibody evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of blocking monoclonal antibody evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:54:44Z
has_obo_namespace: eco
id: ECO:0001150

Axiom
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: definition
annotated target: A type of blocking monoclonal antibody evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

blocking peptide evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking peptide evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking peptide evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of blocking peptide evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T15:55:33Z
has_obo_namespace: eco
id: ECO:0001151

Axiom
annotated source: blocking peptide evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: blocking peptide evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: blocking peptide evidence used in manual assertion
annotated property: definition
annotated target: A type of blocking peptide evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

blocking polyclonal antibody evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking polyclonal antibody evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking polyclonal antibody evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of blocking polyclonal antibody evidence tthat is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:01:39Z
has_obo_namespace: eco
id: ECO:0001152

Axiom
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: definition
annotated target: A type of blocking polyclonal antibody evidence tthat is used in a manual assertion.
database_cross_reference: ECO:MCC

blood test evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blood test evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: blood test evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of blood test evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:02:47Z
has_obo_namespace: eco
id: ECO:0001153

Axiom
annotated source: blood test evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: blood test evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: blood test evidence used in manual assertion
annotated property: definition
annotated target: A type of blood test evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

Boyden chamber assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Boyden chamber assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Boyden chamber assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Boyden chamber assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:03:53Z
has_obo_namespace: eco
id: ECO:0001154

Axiom
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: definition
annotated target: A type of Boyden chamber assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

bromodeoxyuridine incorporation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: bromodeoxyuridine incorporation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: bromodeoxyuridine incorporation assay evidence
subclass of: nucleotide analog incorporation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of BrdU incorporation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:04:49Z
has_obo_namespace: eco
id: ECO:0001155

Axiom
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: nucleotide analog incorporation evidence used in manual assertion
is inferred: true

Axiom
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of BrdU incorporation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

caspase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: caspase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: caspase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of caspase assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:06:06Z
has_obo_namespace: eco
id: ECO:0001156

Axiom
annotated source: caspase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: caspase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: caspase assay evidence used in manual assertion
annotated property: definition
annotated target: A type of caspase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

cell counting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell counting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cell counting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cell counting evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:07:13Z
has_obo_namespace: eco
id: ECO:0001157

Axiom
annotated source: cell counting evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: cell counting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cell counting evidence used in manual assertion
annotated property: definition
annotated target: A type of cell counting evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

cell permeability assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell permeability assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cell permeability assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cell permeability assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:07:58Z
has_obo_namespace: eco
id: ECO:0001158

Axiom
annotated source: cell permeability assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: cell permeability assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cell permeability assay evidence used in manual assertion
annotated property: definition
annotated target: A type of cell permeability assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: carboxyfluorescein diacetate succinimidyl ester staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: carboxyfluorescein diacetate succinimidyl ester staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:08:54Z
has_obo_namespace: eco
id: ECO:0001159

Axiom
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: definition
annotated target: A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

chemiluminescence-linked immunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chemiluminescence-linked immunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: chemiluminescence-linked immunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:09:55Z
has_obo_namespace: eco
id: ECO:0001160

Axiom
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: definition
annotated target: A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

chimeric protein evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chimeric protein evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: mutant phenotype evidence used in manual assertion
subclass of: chimeric protein evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chimeric protein evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:10:40Z
has_obo_namespace: eco
id: ECO:0001161

Axiom
annotated source: chimeric protein evidence used in manual assertion
annotated property: subClassOf
annotated target: mutant phenotype evidence used in manual assertion
is inferred: true

Axiom
annotated source: chimeric protein evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: chimeric protein evidence used in manual assertion
annotated property: definition
annotated target: A type of chimeric protein evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

co-electrophoresis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-electrophoresis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: co-electrophoresis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of co-electrophoresis evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:11:22Z
has_obo_namespace: eco
id: ECO:0001162

Axiom
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: definition
annotated target: A type of co-electrophoresis evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

co-localization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-localization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: co-localization evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of co-localization evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:12:04Z
has_obo_namespace: eco
id: ECO:0001163

Axiom
annotated source: co-localization evidence used in manual assertion
annotated property: subClassOf
annotated target: imaging assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: co-localization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: co-localization evidence used in manual assertion
annotated property: definition
annotated target: A type of co-localization evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

co-sedimentation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-sedimentation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: co-sedimentation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of co-sedimentation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:12:55Z
has_obo_namespace: eco
id: ECO:0001164

Axiom
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of co-sedimentation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

colony counting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: colony counting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: colony counting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of colony counting evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:14:57Z
has_obo_namespace: eco
id: ECO:0001165

Axiom
annotated source: colony counting evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: colony counting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: colony counting evidence used in manual assertion
annotated property: definition
annotated target: A type of colony counting evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

comet assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: comet assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: comet assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of comet assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:16:01Z
has_obo_namespace: eco
id: ECO:0001166

Axiom
annotated source: comet assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: comet assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: comet assay evidence used in manual assertion
annotated property: definition
annotated target: A type of comet assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

conditional knockin evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: conditional knockin evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: conditional knockin evidence
subclass of: knockin evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of conditional knockin evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:17:22Z
has_obo_namespace: eco
id: ECO:0001167

Axiom
annotated source: conditional knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: knockin evidence used in manual assertion
is inferred: true

Axiom
annotated source: conditional knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: conditional knockin evidence used in manual assertion
annotated property: definition
annotated target: A type of conditional knockin evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

conditional knockout evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: conditional knockout evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: conditional knockout evidence
subclass of: knockout evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of conditional knockout evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:17:58Z
has_obo_namespace: eco
id: ECO:0001168

Axiom
annotated source: conditional knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: knockout evidence used in manual assertion
is inferred: true

Axiom
annotated source: conditional knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: conditional knockout evidence used in manual assertion
annotated property: definition
annotated target: A type of conditional knockout evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

constitutively active mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: constitutively active mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: constitutively active mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of constitutively active mutant evidence that is used a in manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:20:23Z
has_obo_namespace: eco
id: ECO:0001169

Axiom
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: definition
annotated target: A type of constitutively active mutant evidence that is used a in manual assertion.
database_cross_reference: ECO:MCC

cross-linking evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cross-linking evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: cross-linking evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cross-linking evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:21:29Z
has_obo_namespace: eco
id: ECO:0001170

Axiom
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cross-linking evidence used in manual assertion
annotated property: definition
annotated target: A type of cross-linking evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

crystallography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: crystallography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: crystallography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of crystallography evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:22:20Z
has_obo_namespace: eco
id: ECO:0001171

Axiom
annotated source: crystallography evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: crystallography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: crystallography evidence used in manual assertion
annotated property: definition
annotated target: A type of crystallography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

cytochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cytochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: cytochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cytochemistry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:23:10Z
has_obo_namespace: eco
id: ECO:0001172

Axiom
annotated source: cytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: cytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cytochemistry evidence used in manual assertion
annotated property: definition
annotated target: A type of cytochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

cytochrome C release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cytochrome C release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cytochrome C release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cytochrome C release assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:23:53Z
has_obo_namespace: eco
id: ECO:0001173

Axiom
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: definition
annotated target: A type of cytochrome C release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

4',6-diamidino-2-phenylindole staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 4',6-diamidino-2-phenylindole staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 4',6-diamidino-2-phenylindole staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:25:03Z
has_obo_namespace: eco
id: ECO:0001174

Axiom
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: definition
annotated target: A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

deletion evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: deletion evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: deletion evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of deletion evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:25:54Z
has_obo_namespace: eco
id: ECO:0001175

Axiom
annotated source: deletion evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: deletion evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: deletion evidence used in manual assertion
annotated property: definition
annotated target: A type of deletion evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

DNA laddering assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA laddering assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: DNA laddering assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNA laddering assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:26:46Z
has_obo_namespace: eco
id: ECO:0001176

Axiom
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: definition
annotated target: A type of DNA laddering assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

RNA dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: RNA dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of RNA dot blot assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:45:52Z
has_obo_namespace: eco
id: ECO:0001177

Axiom
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: definition
annotated target: A type of RNA dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

RNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: RNA microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of RNA microarray evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:46:50Z
has_obo_namespace: eco
id: ECO:0001178

Axiom
annotated source: RNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: RNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: RNA microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of RNA microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

dominant-negative mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: dominant-negative mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: dominant-negative mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of dominant-negative mutant evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:47:44Z
has_obo_namespace: eco
id: ECO:0001179

Axiom
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: definition
annotated target: A type of dominant-negative mutant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

eTag assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: eTag assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: eTag assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of eTag assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T16:59:39Z
has_obo_namespace: eco
id: ECO:0001180

Axiom
annotated source: eTag assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: eTag assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: eTag assay evidence used in manual assertion
annotated property: definition
annotated target: A type of eTag assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

filter binding assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: filter binding assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: filter binding assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of filter binding assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:03:28Z
has_obo_namespace: eco
id: ECO:0001181

Axiom
annotated source: filter binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: filter binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: filter binding assay evidence used in manual assertion
annotated property: definition
annotated target: A type of filter binding assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

fluorescence in situ hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence in situ hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: fluorescence in situ hybridization evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of fluorescence in situ hybridization evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:04:20Z
has_obo_namespace: eco
id: ECO:0001182

Axiom
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: definition
annotated target: A type of fluorescence in situ hybridization evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

fluorescence resonance energy transfer evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence resonance energy transfer evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: fluorescence resonance energy transfer evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of fluorescence resonance energy transfer evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:05:22Z
has_obo_namespace: eco
id: ECO:0001183

Axiom
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: definition
annotated target: A type of fluorescence resonance energy transfer evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

gel-filtration evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: gel-filtration evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: gel-filtration evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of gel-filtration evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:06:14Z
has_obo_namespace: eco
id: ECO:0001184

Axiom
annotated source: gel-filtration evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: gel-filtration evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: gel-filtration evidence used in manual assertion
annotated property: definition
annotated target: A type of gel-filtration evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

histochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: histochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: histochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of histochemistry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:07:19Z
has_obo_namespace: eco
id: ECO:0001185

Axiom
annotated source: histochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: histochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: histochemistry evidence used in manual assertion
annotated property: definition
annotated target: A type of histochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

immunocytochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunocytochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: immunocytochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunocytochemistry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:12:39Z
has_obo_namespace: eco
id: ECO:0001186

Axiom
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: definition
annotated target: A type of immunocytochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

histology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: histology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: histology evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of histology evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:09:50Z
has_obo_namespace: eco
id: ECO:0001187

Axiom
annotated source: histology evidence used in manual assertion
annotated property: subClassOf
annotated target: imaging assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: histology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: histology evidence used in manual assertion
annotated property: definition
annotated target: A type of histology evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

immunodepletion evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunodepletion evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: immunodepletion evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunodepletion evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:16:19Z
has_obo_namespace: eco
id: ECO:0001188

Axiom
annotated source: immunodepletion evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunodepletion evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunodepletion evidence used in manual assertion
annotated property: definition
annotated target: A type of immunodepletion evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

immunohistochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunohistochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: immunohistochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunohistochemistry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:17:22Z
has_obo_namespace: eco
id: ECO:0001189

Axiom
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: definition
annotated target: A type of immunohistochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro acetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro acetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro acetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro acetylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:19:24Z
has_obo_namespace: eco
id: ECO:0001190

Axiom
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro acetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro cleavage assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro cleavage assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro cleavage assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro cleavage assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:21:22Z
has_obo_namespace: eco
id: ECO:0001191

Axiom
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro cleavage assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro deacetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro deacetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro deacetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro deacetylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:23:42Z
has_obo_namespace: eco
id: ECO:0001192

Axiom
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro deacetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro defarnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro defarnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro defarnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro defarnesylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:25:45Z
has_obo_namespace: eco
id: ECO:0001193

Axiom
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro defarnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro demethylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro demethylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro demethylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro demethylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:27:00Z
has_obo_namespace: eco
id: ECO:0001194

Axiom
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro demethylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro desumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro desumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro desumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro desumoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:29:01Z
has_obo_namespace: eco
id: ECO:0001195

Axiom
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro desumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro deubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro deubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro deubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro deubiquitination assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:29:48Z
has_obo_namespace: eco
id: ECO:0001196

Axiom
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro deubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro farnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro farnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro farnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro farnesylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:30:50Z
has_obo_namespace: eco
id: ECO:0001197

Axiom
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro farnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro methylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro methylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro methylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro methylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:31:54Z
has_obo_namespace: eco
id: ECO:0001198

Axiom
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro methylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro palmitoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro palmitoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro palmitoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro palmitoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:32:57Z
has_obo_namespace: eco
id: ECO:0001199

Axiom
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro palmitoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro phosphatase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro phosphatase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro phosphatase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro phosphatase assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:34:16Z
has_obo_namespace: eco
id: ECO:0001200

Axiom
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro phosphatase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro polyADP-ribosylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro polyADP-ribosylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro polyADP-ribosylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:34:57Z
has_obo_namespace: eco
id: ECO:0001201

Axiom
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro protein kinase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro protein kinase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro protein kinase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro protein kinase assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:39:57Z
has_obo_namespace: eco
id: ECO:0001202

Axiom
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro protein kinase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro sumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro sumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro sumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro sumoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:41:25Z
has_obo_namespace: eco
id: ECO:0001203

Axiom
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro sumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro transcription assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro transcription assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro transcription assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro transcription assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:44:40Z
has_obo_namespace: eco
id: ECO:0001204

Axiom
annotated source: in vitro transcription assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro transcription assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro transcription assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro transcription assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro translation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro translation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro translation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro translation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:46:30Z
has_obo_namespace: eco
id: ECO:0001205

Axiom
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro translation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vitro ubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro ubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro ubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vitro ubiquitination assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:47:18Z
has_obo_namespace: eco
id: ECO:0001206

Axiom
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vitro ubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo acetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo acetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo acetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo acetylation assay evidence that is used in manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:48:56Z
has_obo_namespace: eco
id: ECO:0001207

Axiom
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo acetylation assay evidence that is used in manual assertion.
database_cross_reference: ECO:MCC

in vivo cleavage assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo cleavage assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo cleavage assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo cleavage assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:49:43Z
has_obo_namespace: eco
id: ECO:0001208

Axiom
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo cleavage assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo deacetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo deacetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo deacetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo deacetylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:50:47Z
has_obo_namespace: eco
id: ECO:0001209

Axiom
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo deacetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo defarnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo defarnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo defarnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo defarnesylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:52:41Z
has_obo_namespace: eco
id: ECO:0001210

Axiom
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo defarnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo demethylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo demethylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo demethylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo demethylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:53:40Z
has_obo_namespace: eco
id: ECO:0001211

Axiom
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo demethylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo desumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo desumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo desumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo desumoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:54:36Z
has_obo_namespace: eco
id: ECO:0001212

Axiom
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo desumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo deubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo deubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo deubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo deubiquitination assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:56:28Z
has_obo_namespace: eco
id: ECO:0001213

Axiom
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo deubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo farnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo farnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo farnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo farnesylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T17:57:18Z
has_obo_namespace: eco
id: ECO:0001214

Axiom
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo farnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo methylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo methylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo methylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo methylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:03:16Z
has_obo_namespace: eco
id: ECO:0001215

Axiom
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo methylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo palmitoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo palmitoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo palmitoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo palmitoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:04:07Z
has_obo_namespace: eco
id: ECO:0001216

Axiom
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo palmitoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo phosphatase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo phosphatase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo phosphatase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo phosphatase assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:06:08Z
has_obo_namespace: eco
id: ECO:0001217

Axiom
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo phosphatase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo protein kinase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo protein kinase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo protein kinase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo protein kinase assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:07:58Z
has_obo_namespace: eco
id: ECO:0001218

Axiom
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo protein kinase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo sumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo sumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo sumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo sumoylation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:09:49Z
has_obo_namespace: eco
id: ECO:0001219

Axiom
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo sumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo transcription assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo transcription assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo transcription assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo transcription assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:11:20Z
has_obo_namespace: eco
id: ECO:0001220

Axiom
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo transcription assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo translation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo translation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo translation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo translation assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:42:50Z
has_obo_namespace: eco
id: ECO:0001221

Axiom
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo translation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

in vivo ubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo ubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo ubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo ubiquitination assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:44:11Z
has_obo_namespace: eco
id: ECO:0001222

Axiom
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo ubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

induced mutation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: induced mutation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: induced mutation evidence
subclass of: genetic transformation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of induced mutation evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:45:56Z
has_obo_namespace: eco
id: ECO:0001223

Axiom
annotated source: induced mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: genetic transformation evidence used in manual assertion
is inferred: true

Axiom
annotated source: induced mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: induced mutation evidence used in manual assertion
annotated property: definition
annotated target: A type of induced mutation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

knockin evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: knockin evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: knockin evidence
subclass of: genetic transformation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of knockin evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:49:35Z
has_obo_namespace: eco
id: ECO:0001224

Axiom
annotated source: knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: genetic transformation evidence used in manual assertion
is inferred: true

Axiom
annotated source: knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: knockin evidence used in manual assertion
annotated property: definition
annotated target: A type of knockin evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

knockout evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: knockout evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: knockout evidence
subclass of: genetic transformation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of knockout evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:51:31Z
has_obo_namespace: eco
id: ECO:0001225

Axiom
annotated source: knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: genetic transformation evidence used in manual assertion
is inferred: true

Axiom
annotated source: knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: knockout evidence used in manual assertion
annotated property: definition
annotated target: A type of knockout evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

lipid binding assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: lipid binding assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: lipid binding assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of lipid binding assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:52:19Z
has_obo_namespace: eco
id: ECO:0001226

Axiom
annotated source: lipid binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: lipid binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: lipid binding assay evidence used in manual assertion
annotated property: definition
annotated target: A type of lipid binding assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

luminescence-based mammalian interactome mapping assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: luminescence-based mammalian interactome mapping assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: luminescence-based mammalian interactome mapping assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of LUMIER assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:55:16Z
has_obo_namespace: eco
id: ECO:0001227

Axiom
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: definition
annotated target: A type of LUMIER assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

macroscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: macroscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: macroscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of macroscopy evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:56:21Z
has_obo_namespace: eco
id: ECO:0001228

Axiom
annotated source: macroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: macroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: macroscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of macroscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

mammalian 2-hybrid assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mammalian 2-hybrid assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: mammalian 2-hybrid assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of mammalian 2-hybrid assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:57:25Z
has_obo_namespace: eco
id: ECO:0001229

Axiom
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: definition
annotated target: A type of mammalian 2-hybrid assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: mass spectrometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of mass spectrometry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T18:59:50Z
has_obo_namespace: eco
id: ECO:0001230

Axiom
annotated source: mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: mass spectrometry evidence used in manual assertion
annotated property: definition
annotated target: A type of mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

medical imaging evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: medical imaging evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: medical imaging evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of medical imaging evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:04:43Z
has_obo_namespace: eco
id: ECO:0001231

Axiom
annotated source: medical imaging evidence used in manual assertion
annotated property: subClassOf
annotated target: imaging assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: medical imaging evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: medical imaging evidence used in manual assertion
annotated property: definition
annotated target: A type of medical imaging evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of microscopy evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:05:37Z
has_obo_namespace: eco
id: ECO:0001232

Axiom
annotated source: microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: imaging assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

motility wound healing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motility wound healing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: motility wound healing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of motility wound healing assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:08:13Z
has_obo_namespace: eco
id: ECO:0001233

Axiom
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: definition
annotated target: A type of motility wound healing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

MTS assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: MTS assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: MTS assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of MTS assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:08:51Z
has_obo_namespace: eco
id: ECO:0001234

Axiom
annotated source: MTS assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: MTS assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: MTS assay evidence used in manual assertion
annotated property: definition
annotated target: A type of MTS assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

MTT assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: MTT assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: MTT assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of MTT assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:13:17Z
has_obo_namespace: eco
id: ECO:0001235

Axiom
annotated source: MTT assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: MTT assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: MTT assay evidence used in manual assertion
annotated property: definition
annotated target: A type of MTT assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

multiplex bead-based immunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: multiplex bead-based immunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: multiplex bead-based immunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of multiplex bead-based immunoassay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:14:03Z
has_obo_namespace: eco
id: ECO:0001236

Axiom
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: definition
annotated target: A type of multiplex bead-based immunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

natural variation mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: natural variation mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: natural variation mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of natural variation mutant evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:15:48Z
has_obo_namespace: eco
id: ECO:0001237

Axiom
annotated source: natural variation mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: natural variation mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: natural variation mutant evidence used in manual assertion
annotated property: definition
annotated target: A type of natural variation mutant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

nuclear magnetic resonance spectroscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclear magnetic resonance spectroscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: nuclear magnetic resonance spectroscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:18:50Z
has_obo_namespace: eco
id: ECO:0001238

Axiom
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

nuclear fragmentation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclear fragmentation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: nuclear fragmentation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of nuclear fragmentation evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:19:55Z
has_obo_namespace: eco
id: ECO:0001239

Axiom
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: definition
annotated target: A type of nuclear fragmentation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

nuclease protection assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclease protection assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: nuclease protection assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of nuclease protection assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:23:41Z
has_obo_namespace: eco
id: ECO:0001240

Axiom
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: definition
annotated target: A type of nuclease protection assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

nucleotide analog incorporation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nucleotide analog incorporation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: nucleotide analog incorporation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of nucleotide analog incorporation evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:25:17Z
has_obo_namespace: eco
id: ECO:0001241

Axiom
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: definition
annotated target: A type of nucleotide analog incorporation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

phage display evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phage display evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: phage display evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of phage display evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:27:17Z
has_obo_namespace: eco
id: ECO:0001242

Axiom
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: phage display evidence used in manual assertion
annotated property: definition
annotated target: A type of phage display evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

phosphoamino acid analysis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phosphoamino acid analysis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: phosphoamino acid analysis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of phosphoamino acid analysis evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:28:59Z
has_obo_namespace: eco
id: ECO:0001243

Axiom
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: definition
annotated target: A type of phosphoamino acid analysis evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

peptide affinity enrichment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: peptide affinity enrichment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: peptide affinity enrichment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of peptide affinity enrichment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T19:30:13Z
has_obo_namespace: eco
id: ECO:0001244

Axiom
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: definition
annotated target: A type of peptide affinity enrichment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

peptide array evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: peptide array evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: peptide array evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of peptide array evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:16:45Z
has_obo_namespace: eco
id: ECO:0001245

Axiom
annotated source: peptide array evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: peptide array evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: peptide array evidence used in manual assertion
annotated property: definition
annotated target: A type of peptide array evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

physical examination evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: physical examination evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical examination evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of physical examination evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:17:27Z
has_obo_namespace: eco
id: ECO:0001246

Axiom
annotated source: physical examination evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: physical examination evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: physical examination evidence used in manual assertion
annotated property: definition
annotated target: A type of physical examination evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

point mutation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: point mutation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: point mutation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of point mutation evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:18:10Z
has_obo_namespace: eco
id: ECO:0001247

Axiom
annotated source: point mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: point mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: point mutation evidence used in manual assertion
annotated property: definition
annotated target: A type of point mutation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

propidium iodide staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: propidium iodide staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: propidium iodide staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of propidium iodide staining evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:18:58Z
has_obo_namespace: eco
id: ECO:0001248

Axiom
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: definition
annotated target: A type of propidium iodide staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

fluorescence evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: fluorescence evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein detection by fluorescence evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:19:35Z
has_obo_namespace: eco
id: ECO:0001249

Axiom
annotated source: fluorescence evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: fluorescence evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: fluorescence evidence used in manual assertion
annotated property: definition
annotated target: A type of protein detection by fluorescence evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein dot blot assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:20:30Z
has_obo_namespace: eco
id: ECO:0001250

Axiom
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: definition
annotated target: A type of protein dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein microarray evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:21:11Z
has_obo_namespace: eco
id: ECO:0001251

Axiom
annotated source: protein microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of protein microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein sequencing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein sequencing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein sequencing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of protein sequencing assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:22:08Z
has_obo_namespace: eco
id: ECO:0001252

Axiom
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: definition
annotated target: A type of protein sequencing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

quantitative mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: quantitative mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: quantitative mass spectrometry evidence
subclass of: mass spectrometry evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of quantitative mass spectrometry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:23:15Z
has_obo_namespace: eco
id: ECO:0001253

Axiom
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: mass spectrometry evidence used in manual assertion
is inferred: true

Axiom
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: definition
annotated target: A type of quantitative mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

radioisotope assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: radioisotope assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: radioisotope assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of radioisotope assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:27:05Z
has_obo_namespace: eco
id: ECO:0001254

Axiom
annotated source: radioisotope assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: radioisotope assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: radioisotope assay evidence used in manual assertion
annotated property: definition
annotated target: A type of radioisotope assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

radioimmunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: radioimmunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: radioimmunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of radioimmunoassay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:28:04Z
has_obo_namespace: eco
id: ECO:0001255

Axiom
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: definition
annotated target: A type of radioimmunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

imaging assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: imaging assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: imaging assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of imaging assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:00Z
has_obo_namespace: eco
id: ECO:0001256

Axiom
annotated source: imaging assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: imaging assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: imaging assay evidence used in manual assertion
annotated property: definition
annotated target: A type of imaging assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

restriction fragment detection evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: restriction fragment detection evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: restriction fragment detection evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of restriction fragment detection evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:05Z
has_obo_namespace: eco
id: ECO:0001257

Axiom
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: definition
annotated target: A type of restriction fragment detection evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

spectrophotometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spectrophotometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: spectrophotometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of spectrophotometry evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:09Z
has_obo_namespace: eco
id: ECO:0001258

Axiom
annotated source: spectrophotometry evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: spectrophotometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: spectrophotometry evidence used in manual assertion
annotated property: definition
annotated target: A type of spectrophotometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

syngeneic transplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: syngeneic transplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: syngeneic transplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of syngeneic transplantation experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:13Z
has_obo_namespace: eco
id: ECO:0001259

Axiom
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of syngeneic transplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

xenotransplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: xenotransplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: xenotransplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of xenotransplantation experiment evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:18Z
has_obo_namespace: eco
id: ECO:0001260

Axiom
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of xenotransplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

WST-1 assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: WST-1 assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: WST-1 assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of WST-1 assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:22Z
has_obo_namespace: eco
id: ECO:0001261

Axiom
annotated source: WST-1 assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: WST-1 assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: WST-1 assay evidence used in manual assertion
annotated property: definition
annotated target: A type of WST-1 assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

wound healing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: wound healing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: wound healing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of wound healing assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:26Z
has_obo_namespace: eco
id: ECO:0001262

Axiom
annotated source: wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: wound healing assay evidence used in manual assertion
annotated property: definition
annotated target: A type of wound healing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

urine test evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: urine test evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: urine test evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of urine test evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:30Z
has_obo_namespace: eco
id: ECO:0001263

Axiom
annotated source: urine test evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: urine test evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: urine test evidence used in manual assertion
annotated property: definition
annotated target: A type of urine test evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of TUNEL assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:34Z
has_obo_namespace: eco
id: ECO:0001264

Axiom
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: definition
annotated target: A type of TUNEL assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

tryptic phosphopeptide mapping assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: tryptic phosphopeptide mapping assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: tryptic phosphopeptide mapping assay evidence
subclass of: mass spectrometry evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:38Z
has_obo_namespace: eco
id: ECO:0001265

Axiom
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: mass spectrometry evidence used in manual assertion
is inferred: true

Axiom
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: definition
annotated target: A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

transgenic organism evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transgenic organism evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transgenic organism evidence
subclass of: genetic transformation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of transgenic organism evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:41Z
has_obo_namespace: eco
id: ECO:0001266

Axiom
annotated source: transgenic organism evidence used in manual assertion
annotated property: subClassOf
annotated target: genetic transformation evidence used in manual assertion
is inferred: true

Axiom
annotated source: transgenic organism evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: transgenic organism evidence used in manual assertion
annotated property: definition
annotated target: A type of transgenic organism evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

tissue microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: tissue microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: array experiment evidence used in manual assertion
subclass of: tissue microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of tissue microarray evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:45Z
has_obo_namespace: eco
id: ECO:0001267

Axiom
annotated source: tissue microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: array experiment evidence used in manual assertion
is inferred: true

Axiom
annotated source: tissue microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: tissue microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of tissue microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

TACE activity assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: TACE activity assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: TACE activity assay evidence
subclass of: enzyme assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of TACE activity assay evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:49Z
has_obo_namespace: eco
id: ECO:0001268

Axiom
annotated source: TACE activity assay evidence used in manual assertion
annotated property: subClassOf
annotated target: enzyme assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: TACE activity assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: TACE activity assay evidence used in manual assertion
annotated property: definition
annotated target: A type of TACE activity assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

surface plasmon resonance evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: surface plasmon resonance evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: surface plasmon resonance evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of surface plasmon resonance evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:54Z
has_obo_namespace: eco
id: ECO:0001269

Axiom
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: definition
annotated target: A type of surface plasmon resonance evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

restriction landmark genomic scanning evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: restriction landmark genomic scanning evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: restriction landmark genomic scanning evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of restriction landmark genome scanning evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:29:58Z
has_obo_namespace: eco
id: ECO:0001270

Axiom
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: definition
annotated target: A type of restriction landmark genome scanning evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

resonant mirror biosensor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: resonant mirror biosensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: resonant mirror biosensor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of resonant mirror biosensor evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T20:30:02Z
has_obo_namespace: eco
id: ECO:0001271

Axiom
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: definition
annotated target: A type of resonant mirror biosensor evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

high-performance liquid chromatography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: high-performance liquid chromatography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: high-performance liquid chromatography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of high-performance liquid chromatography evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-16T20:49:54Z
has_obo_namespace: eco
id: ECO:0001272

Axiom
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: definition
annotated target: A type of high-performance liquid chromatography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

ectopic expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ectopic expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: ectopic expression evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of ectopic expression evidence that is used in a manual assertion.
created by: mchibucos
creation date: 2014-03-17T22:34:56Z
has_obo_namespace: eco
id: ECO:0001273

Axiom
annotated source: ectopic expression evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: ectopic expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: ectopic expression evidence used in manual assertion
annotated property: definition
annotated target: A type of ectopic expression evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC

protein-binding microarray evidence
type: Class
subclass of: oligonucleotide microarray evidence
subclass of: nucleic acid binding evidence
definition: A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites.
created by: swolfish
creation date: 2015-06-05T14:36:00Z
has_exact_synonym: PBM evidence
has_obo_namespace: eco
id: ECO:0001601

Axiom
annotated source: protein-binding microarray evidence
annotated property: definition
annotated target: A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites.
database_cross_reference: PMID:22146299

in vivo cell staining evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death.
created by: swolfish
creation date: 2015-06-08T11:51:37Z
has_obo_namespace: eco
has_related_synonym: vital staining evidence
id: ECO:0001603

Axiom
annotated source: in vivo cell staining evidence
annotated property: definition
annotated target: A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death.
database_cross_reference: GOC:MAH

Axiom
annotated source: in vivo cell staining evidence
annotated property: has_related_synonym
annotated target: vital staining evidence
database_cross_reference: GOC:MAH

alkaline phosphatase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing.
created by: swolfish
creation date: 2015-10-29T15:53:45Z
has_obo_namespace: eco
has_related_synonym: SEAP reporter assay
has_related_synonym: Secreted Embryonic Alkaline Phosphatase reporter assay
id: ECO:0001801
comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane.

Axiom
annotated source: alkaline phosphatase reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11823238

beta-galactosidase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.
created by: swolfish
creation date: 2015-10-29T15:57:10Z
has_exact_synonym: beta-gal reporter gene assay
has_obo_namespace: eco
has_related_synonym: lacZ reporter gene assay
id: ECO:0001802
comment: The assay is often performed using a plasmid borne construction on a lacZ strain.

Axiom
annotated source: beta-galactosidase reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20439410

chloramphenicol acetyltransferase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic.
created by: swolfish
creation date: 2015-10-29T16:06:42Z
has_exact_synonym: CAT reporter gene assay
has_obo_namespace: eco
id: ECO:0001803
comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present.

Axiom
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic.
database_cross_reference: ECO:SW
database_cross_reference: PMID:1630936

Axiom
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence
annotated property: has_exact_synonym
annotated target: CAT reporter gene assay
database_cross_reference: PMID:1630936

beta-glucuronidase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection.
created by: swolfish
creation date: 2015-10-29T16:11:02Z
has_exact_synonym: GUS reporter gene assay
has_obo_namespace: eco
id: ECO:0001804

Axiom
annotated source: beta-glucuronidase reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection.
database_cross_reference: ECO:SW

luciferase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence.
created by: swolfish
creation date: 2015-10-29T16:15:39Z
has_obo_namespace: eco
id: ECO:0001805

Axiom
annotated source: luciferase reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17623934

chromatin immunoprecipitation- exonuclease evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
definition: A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data.
created by: swolfish
creation date: 2015-10-29T16:37:36Z
has_exact_synonym: ChIP-exo
has_obo_namespace: eco
id: ECO:0001806

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence
annotated property: definition
annotated target: A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data.
database_cross_reference: ECO:SW
database_cross_reference: PMID:25249628

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence
annotated property: has_exact_synonym
annotated target: ChIP-exo
database_cross_reference: PMID:25249628

electrophoretic mobility shift assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: electrophoretic mobility shift assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophoretic mobility shift assay evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of electrophoretic mobility shift that is used in a manual assertion.
created by: swolfish
creation date: 2015-11-03T12:13:17Z
has_obo_namespace: eco
id: ECO:0001807

Axiom
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: definition
annotated target: A type of electrophoretic mobility shift that is used in a manual assertion.
database_cross_reference: ECO:SW

reverse transcription polymerase chain reaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: reverse transcription polymerase chain reaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: reverse transcription polymerase chain reaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion.
created by: swolfish
creation date: 2015-11-03T12:22:46Z
has_obo_namespace: eco
id: ECO:0001808

Axiom
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: definition
annotated target: A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion.
database_cross_reference: ECO:SW

DNA affinity chromatography evidence
type: Class
subclass of: chromatography evidence
definition: A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins.
created by: swolfish
creation date: 2015-11-03T13:48:49Z
has_obo_namespace: eco
has_related_synonym: DNA affinity purification
id: ECO:0001809
comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture.

Axiom
annotated source: DNA affinity chromatography evidence
annotated property: definition
annotated target: A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11694305
database_cross_reference: PMID:11725488

DNAse footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions.
created by: swolfish
creation date: 2015-11-03T13:55:15Z
has_obo_namespace: eco
has_related_synonym: DNase protection
id: ECO:0001810
comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites.

Axiom
annotated source: DNAse footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions.
database_cross_reference: ECO:SW
database_cross_reference: PMID:212715
database_cross_reference: PMID:22194258

fluorescence anisotropy evidence
type: Class
subclass of: fluorescence evidence
definition: A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules.
created by: swolfish
creation date: 2015-11-03T14:01:36Z
has_exact_synonym: FA
has_obo_namespace: eco
has_related_synonym: FP
has_related_synonym: Fluorescence polarization
id: ECO:0001811
comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous.

Axiom
annotated source: fluorescence anisotropy evidence
annotated property: definition
annotated target: A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20232898

Axiom
annotated source: fluorescence anisotropy evidence
annotated property: has_related_synonym
annotated target: FP
database_cross_reference: PMCID:3277431
database_cross_reference: PMID:20232898

Axiom
annotated source: fluorescence anisotropy evidence
annotated property: has_related_synonym
annotated target: Fluorescence polarization
database_cross_reference: PMCID:3277431
database_cross_reference: PMID:20232898

genomic systematic evolution of ligands by exponential amplification evidence
type: Class
subclass of: systematic evolution of ligands by exponential amplification evidence
definition: A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing.
created by: swolfish
creation date: 2015-11-03T14:05:37Z
has_exact_synonym: genomic SELEX
has_obo_namespace: eco
id: ECO:0001812
comment: The reported sequences should always be verified for presence in the genome sequence.

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence
annotated property: definition
annotated target: A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20541015
database_cross_reference: PMID:21720957

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence
annotated property: has_exact_synonym
annotated target: genomic SELEX
database_cross_reference: PMID:20541015

heteronuclear single quantum coherence spectroscopy evidence
type: Class
subclass of: nuclear magnetic resonance spectroscopy evidence
definition: A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum.
created by: swolfish
creation date: 2015-11-03T14:11:06Z
has_exact_synonym: HSQC
has_obo_namespace: eco
id: ECO:0001813
comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site.

Axiom
annotated source: heteronuclear single quantum coherence spectroscopy evidence
annotated property: definition
annotated target: A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum.
database_cross_reference: ECO:SW
database_cross_reference: PMID:19856946

Axiom
annotated source: heteronuclear single quantum coherence spectroscopy evidence
annotated property: has_exact_synonym
annotated target: HSQC
database_cross_reference: PMID:19856946

methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA.
created by: swolfish
creation date: 2015-11-03T14:24:04Z
has_obo_namespace: eco
has_related_synonym: MPE Fe(II) footprinting
has_related_synonym: MPE-EDTA Fe(II) footprinting
has_related_synonym: Methidiumpropyl-EDTA Fe(II) footprinting
id: ECO:0001814

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:6225070

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym
annotated target: MPE Fe(II) footprinting
database_cross_reference: PMID:6225070

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym
annotated target: MPE-EDTA Fe(II) footprinting
database_cross_reference: PMID:6225070

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym
annotated target: Methidiumpropyl-EDTA Fe(II) footprinting
database_cross_reference: PMID:6225070

copper-phenanthroline footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts.
created by: swolfish
creation date: 2015-11-04T08:37:51Z
has_exact_synonym: 1,10-Phenanthroline-copper footprinting
has_obo_namespace: eco
has_related_synonym: OP-Cu Complex
id: ECO:0001815

Axiom
annotated source: copper-phenanthroline footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11691942

Axiom
annotated source: copper-phenanthroline footprinting evidence
annotated property: has_exact_synonym
annotated target: 1,10-Phenanthroline-copper footprinting
database_cross_reference: PMID:1384472

Axiom
annotated source: copper-phenanthroline footprinting evidence
annotated property: has_related_synonym
annotated target: OP-Cu Complex
database_cross_reference: PMID:1384472

green fluorescent protein reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light.
created by: swolfish
creation date: 2015-11-04T08:58:42Z
has_exact_synonym: GFP reporter gene assay
has_obo_namespace: eco
has_related_synonym: GFP promoter fusion
has_related_synonym: green fluorescent protein promoter fusion
id: ECO:0001816

Axiom
annotated source: green fluorescent protein reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11989662

Axiom
annotated source: green fluorescent protein reporter gene assay evidence
annotated property: has_exact_synonym
annotated target: GFP reporter gene assay
database_cross_reference: PMID:11989662

glutathione S-transferase pull-down assay evidence
type: Class
subclass of: protein binding evidence
definition: A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions.
created by: swolfish
creation date: 2015-11-04T09:05:56Z
has_exact_synonym: GST pull-down assay
has_obo_namespace: eco
id: ECO:0001817
comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing.

Axiom
annotated source: glutathione S-transferase pull-down assay evidence
annotated property: definition
annotated target: A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions.
database_cross_reference: ECO:SW
database_cross_reference: PMID:26096507

Axiom
annotated source: glutathione S-transferase pull-down assay evidence
annotated property: has_exact_synonym
annotated target: GST pull-down assay
database_cross_reference: PMID:26096507

hydroxyl-radical footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel.
created by: swolfish
creation date: 2015-11-04T09:20:26Z
has_obo_namespace: eco
id: ECO:0001818

Axiom
annotated source: hydroxyl-radical footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel.
database_cross_reference: ECO:SW
database_cross_reference: PMID:18546600
database_cross_reference: PMID:19378159
database_cross_reference: PMID:3090544

primer extension assay evidence
type: Class
subclass of: transcript expression level evidence
definition: A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA.
created by: swolfish
creation date: 2015-11-04T09:33:18Z
has_obo_namespace: eco
id: ECO:0001819
comment: The most commonly used radiolabel is 32P.

Axiom
annotated source: primer extension assay evidence
annotated property: definition
annotated target: A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23378648

rapid amplification of cDNA ends polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
definition: A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript.
created by: swolfish
creation date: 2015-11-04T09:48:38Z
has_exact_synonym: RACE PCR
has_obo_namespace: eco
id: ECO:0001820
comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression.

Axiom
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence
annotated property: definition
annotated target: A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17498297

Axiom
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence
annotated property: has_exact_synonym
annotated target: RACE PCR
database_cross_reference: PMI:17498297

RNA sequencing assay evidence
type: Class
subclass of: nucleotide sequencing assay evidence
definition: A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms.
created by: swolfish
creation date: 2015-11-04T10:15:57Z
has_exact_synonym: RNA-Seq
has_exact_synonym: WTTS
has_exact_synonym: Whole Transcriptome Shotgun Sequencing
has_obo_namespace: eco
id: ECO:0001821

Axiom
annotated source: RNA sequencing assay evidence
annotated property: definition
annotated target: A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms.
database_cross_reference: ECO:SW
database_cross_reference: PMID:19015660

Axiom
annotated source: RNA sequencing assay evidence
annotated property: has_exact_synonym
annotated target: RNA-Seq
database_cross_reference: PMID:23851085

Axiom
annotated source: RNA sequencing assay evidence
annotated property: has_exact_synonym
annotated target: Whole Transcriptome Shotgun Sequencing
database_cross_reference: PMID:23851085

survival rate analysis evidence
type: Class
subclass of: knockout evidence
definition: A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter.
created by: swolfish
creation date: 2015-11-04T10:48:59Z
has_obo_namespace: eco
id: ECO:0001822

Axiom
annotated source: survival rate analysis evidence
annotated property: definition
annotated target: A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter.
database_cross_reference: ECO:SW

x-ray crystallography evidence
type: Class
subclass of: crystallography evidence
definition: A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules.
created by: swolfish
creation date: 2015-11-04T10:56:22Z
has_obo_namespace: eco
id: ECO:0001823
comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components.

Axiom
annotated source: x-ray crystallography evidence
annotated property: definition
annotated target: A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23135450
database_cross_reference: PMID:24648090

DNA adenine methyltransferase identification evidence
type: Class
subclass of: affinity evidence
definition: A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site.
created by: swolfish
creation date: 2015-11-05T15:56:13Z
has_exact_synonym: DamID
has_obo_namespace: eco
id: ECO:0001824
comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII.

Axiom
annotated source: DNA adenine methyltransferase identification evidence
annotated property: definition
annotated target: A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site.
database_cross_reference: ECO:SW
database_cross_reference: PMID:16938559
database_cross_reference: PMID:17545983
database_cross_reference: PMID:19588092

Axiom
annotated source: DNA adenine methyltransferase identification evidence
annotated property: has_exact_synonym
annotated target: DamID
database_cross_reference: PMID:19588092

isothermal titration calorimetry evidence
type: Class
subclass of: affinity evidence
definition: A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction.
created by: swolfish
creation date: 2015-11-05T16:06:20Z
has_exact_synonym: ITC
has_obo_namespace: eco
id: ECO:0001825
comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)).

Axiom
annotated source: isothermal titration calorimetry evidence
annotated property: definition
annotated target: A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10527727

Axiom
annotated source: isothermal titration calorimetry evidence
annotated property: has_exact_synonym
annotated target: ITC
database_cross_reference: PMID:10527727

ultraviolet light footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts.
created by: swolfish
creation date: 2015-11-05T16:13:36Z
has_obo_namespace: eco
has_related_synonym: UV footprinting
has_related_synonym: ultraviolet footprinting
id: ECO:0001826

Axiom
annotated source: ultraviolet light footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts.
database_cross_reference: ECO:SW
database_cross_reference: PMID:2842760
database_cross_reference: PMID:7602584

premethylation interference footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel.
created by: swolfish
creation date: 2015-11-05T16:16:27Z
has_obo_namespace: eco
has_related_synonym: methylation interference footprinting
id: ECO:0001827

Axiom
annotated source: premethylation interference footprinting evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel.
database_cross_reference: ECO:SW
database_cross_reference: PMID:1583685

visual sequence inspection evidence
type: Class
subclass of: inference from background scientific knowledge used in manual assertion
definition: A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites.
created by: swolfish
creation date: 2015-11-10T14:40:16Z
has_obo_namespace: eco
id: ECO:0001828

Axiom
annotated source: visual sequence inspection evidence
annotated property: definition
annotated target: A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites.
database_cross_reference: ECO:SW

ferric uptake regulator titration assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.
created by: swolfish
creation date: 2015-11-10T14:55:36Z
has_exact_synonym: FURTA
has_obo_namespace: eco
has_related_synonym: Fluorescence polarization
id: ECO:0001829

Axiom
annotated source: ferric uptake regulator titration assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10713425
database_cross_reference: PMID:7642488
database_cross_reference: PMID:8107138

Axiom
annotated source: ferric uptake regulator titration assay evidence
annotated property: has_exact_synonym
annotated target: FURTA
database_cross_reference: PMID:10713425

Axiom
annotated source: ferric uptake regulator titration assay evidence
annotated property: has_related_synonym
annotated target: Fluorescence polarization
database_cross_reference: PMID:15689115

cell viability assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: snadendla
creation date: 2015-02-13T12:08:01Z
has_obo_namespace: eco
id: ECO:0005004

cell proliferation assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: snadendla
creation date: 2015-02-11T16:27:13Z
has_obo_namespace: eco
id: ECO:0005007

DNA synthesis cell proliferation assay evidence
type: Class
subclass of: cell proliferation assay evidence
created by: snadendla
creation date: 2015-02-11T16:30:10Z
has_obo_namespace: eco
id: ECO:0005008

metabolic cell proliferation assay evidence
type: Class
subclass of: cell proliferation assay evidence
created by: snadendla
creation date: 2015-02-11T16:36:33Z
has_obo_namespace: eco
id: ECO:0005009

cell proliferation marker detection assay evidence
type: Class
subclass of: cell proliferation assay evidence
created by: snadendla
creation date: 2015-02-11T16:37:23Z
has_obo_namespace: eco
id: ECO:0005010

ATP bioluminescence assay evidence
type: Class
subclass of: cell proliferation assay evidence
created by: snadendla
creation date: 2015-02-11T16:47:30Z
has_obo_namespace: eco
id: ECO:0005011

cytotoxicity assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: snadendla
creation date: 2015-02-12T11:39:35Z
has_obo_namespace: eco
id: ECO:0005012

in vitro cell based assay evidence
type: Class
subclass of: in vitro assay evidence
created by: snadendla
creation date: 2015-02-12T13:26:55Z
has_obo_namespace: eco
id: ECO:0005014

staining evidence
type: Class
subclass of: direct assay evidence
created by: snadendla
creation date: 2015-02-19T11:48:57Z
has_obo_namespace: eco
id: ECO:0005019

chemotaxis assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: snadendla
creation date: 2015-02-19T13:10:20Z
has_obo_namespace: eco
id: ECO:0005021

genetic transformation evidence
type: Class
subclass of: mutant phenotype evidence
definition: A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA.
created by: snadendla
creation date: 2015-03-16T15:33:50Z
has_obo_namespace: eco
id: ECO:0005027

Axiom
annotated source: genetic transformation evidence
annotated property: definition
annotated target: A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA.
database_cross_reference: ECO:RCT

structure determination evidence
type: Class
subclass of: experimental evidence
created by: snadendla
creation date: 2015-03-20T12:16:17Z
has_obo_namespace: eco
id: ECO:0005031

electron microscopy evidence
type: Class
subclass of: microscopy evidence
subclass of: structure determination evidence
created by: snadendla
creation date: 2015-03-20T12:21:48Z
has_obo_namespace: eco
id: ECO:0005033

apoptotic assay evidence
type: Class
subclass of: in vitro cell based assay evidence
created by: snadendla
creation date: 2015-04-07T21:58:24Z
has_obo_namespace: eco
id: ECO:0005034

in vivo polyADP-ribosylation assay evidence
type: Class
subclass of: in vivo assay evidence
definition: A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo.
created by: snadendla
creation date: 2015-06-15T15:35:05Z
has_obo_namespace: eco
id: ECO:0005500

Axiom
annotated source: in vivo polyADP-ribosylation assay evidence
annotated property: definition
annotated target: A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:2820766
database_cross_reference: SIB:PG

in vivo polyADP-ribosylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo polyADP-ribosylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo polyADP-ribosylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-06-15T15:37:23Z
has_obo_namespace: eco
id: ECO:0005501

Axiom
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

ex vivo assay evidence
type: Class
subclass of: biological assay evidence
definition: A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions.
created by: snadendla
creation date: 2015-06-26T11:46:15Z
has_obo_namespace: eco
id: ECO:0005502

Axiom
annotated source: ex vivo assay evidence
annotated property: definition
annotated target: A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions.
database_cross_reference: ECO:SN
database_cross_reference: PMID:16305312
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf

two-dimensional polyacrylamide gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
definition: A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape.
created by: snadendla
creation date: 2015-10-01T16:23:20Z
has_exact_synonym: 2D PAGE
has_exact_synonym: 2D-PAGE
has_obo_namespace: eco
id: ECO:0005503
comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes.

Axiom
annotated source: two-dimensional polyacrylamide gel electrophoresis evidence
annotated property: definition
annotated target: A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape.
database_cross_reference: ECO:SW
database_cross_reference: url:http://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html

spectrometry evidence
type: Class
subclass of: experimental evidence
created by: snadendla
creation date: 2015-08-14T15:48:08Z
has_obo_namespace: eco
id: ECO:0005504

regular expression motif search evidence
type: Class
subclass of: motif similarity evidence
definition: A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression.
created by: snadendla
creation date: 2015-10-09T13:51:52Z
has_obo_namespace: eco
id: ECO:0005505
comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667).

Axiom
annotated source: regular expression motif search evidence
annotated property: definition
annotated target: A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17337630

missense mutation evidence
type: Class
subclass of: point mutation evidence
created by: snadendla
creation date: 2015-09-18T16:52:34Z
has_obo_namespace: eco
id: ECO:0005506

nonsense mutation evidence
type: Class
subclass of: point mutation evidence
created by: snadendla
creation date: 2015-09-18T16:55:32Z
has_obo_namespace: eco
id: ECO:0005507

silent mutation evidence
type: Class
subclass of: point mutation evidence
created by: snadendla
creation date: 2015-09-18T17:01:12Z
has_obo_namespace: eco
id: ECO:0005508

insertion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:02:03Z
has_obo_namespace: eco
id: ECO:0005509

deletion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:02:45Z
has_obo_namespace: eco
id: ECO:0005510

duplication mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:03:25Z
has_obo_namespace: eco
id: ECO:0005511

frameshift mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:05:49Z
has_obo_namespace: eco
id: ECO:0005512

repeat expansion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:06:34Z
has_obo_namespace: eco
id: ECO:0005513

splice site mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:07:29Z
has_obo_namespace: eco
id: ECO:0005514

translocation mutation evidence
type: Class
subclass of: experimental phenotypic evidence
created by: snadendla
creation date: 2015-09-18T17:08:01Z
has_obo_namespace: eco
id: ECO:0005515

molecule detection assay evidence
type: Class
subclass of: experimental evidence
created by: snadendla
creation date: 2015-09-24T15:54:21Z
has_obo_namespace: eco
id: ECO:0005516

protein detection assay evidence
type: Class
subclass of: molecule detection assay evidence
created by: snadendla
creation date: 2015-09-25T14:27:40Z
has_obo_namespace: eco
id: ECO:0005517

RNA detection assay evidence
type: Class
subclass of: molecule detection assay evidence
created by: snadendla
creation date: 2015-09-25T14:43:53Z
has_obo_namespace: eco
id: ECO:0005518

DNA detection assay evidence
type: Class
subclass of: molecule detection assay evidence
created by: snadendla
creation date: 2015-09-25T19:33:25Z
has_obo_namespace: eco
id: ECO:0005519

interferometric reflectance imaging sensor evidence
type: Class
subclass of: molecule detection assay evidence
definition: A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels.
created by: snadendla
creation date: 2015-09-28T16:01:01Z
has_exact_synonym: IRIS
has_exact_synonym: SRIB
has_exact_synonym: Spectral Reflectance Imaging Biosensor
has_obo_namespace: eco
id: ECO:0005520
comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively.

Axiom
annotated source: interferometric reflectance imaging sensor evidence
annotated property: definition
annotated target: A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21587155
database_cross_reference: PMID:24271115

S1 nuclease protection assay evidence
type: Class
subclass of: nuclease protection assay evidence
definition: A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA.
created by: snadendla
creation date: 2015-09-28T17:16:56Z
has_obo_namespace: eco
id: ECO:0005521

Axiom
annotated source: S1 nuclease protection assay evidence
annotated property: definition
annotated target: A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21390683

DNA dot blot assay evidence
type: Class
subclass of: DNA detection assay evidence
created by: snadendla
creation date: 2015-09-30T10:03:03Z
has_obo_namespace: eco
id: ECO:0005522

DNA dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: DNA dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNA dot blot assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-09-30T10:04:00Z
has_obo_namespace: eco
id: ECO:0005523

Axiom
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: definition
annotated target: A type of DNA dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW

DNA microarray evidence
type: Class
subclass of: DNA detection assay evidence
definition: A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner.
created by: snadendla
creation date: 2015-09-30T10:12:48Z
has_obo_namespace: eco
has_related_synonym: DNA chip
has_related_synonym: DNA-array expression analysis
id: ECO:0005524
comment: The mRNA extraction is performed in induced and non-induced cells and reverse transcribed with different fluorophore labeling for controls and experiment. Fluorescence is measured at two different wavelengths and the ratio between the two fluorescence intensities corresponds to the induction level.

Axiom
annotated source: DNA microarray evidence
annotated property: definition
annotated target: A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17660860

Axiom
annotated source: DNA microarray evidence
annotated property: has_related_synonym
annotated target: DNA chip
database_cross_reference: PMID:10398077

DNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: DNA microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNA microarray evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-09-30T10:13:09Z
has_obo_namespace: eco
id: ECO:0005525

Axiom
annotated source: DNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNA microarray evidence used in manual assertion
annotated property: definition
annotated target: A type of DNA microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
type: Class
subclass of: protein detection assay evidence
definition: A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology.
created by: snadendla
creation date: 2015-09-30T10:51:56Z
has_exact_synonym: MALDI-TOF Mass Spectrometry
has_exact_synonym: MALDI-TOF-MS
has_obo_namespace: eco
id: ECO:0005526
comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available.

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
annotated property: definition
annotated target: A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21964792
database_cross_reference: PMID:25354905

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-09-30T10:58:51Z
has_obo_namespace: eco
id: ECO:0005527

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: definition
annotated target: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW

site-directed mutagenesis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence.
created by: snadendla
creation date: 2015-09-30T11:34:41Z
has_exact_synonym: site directed mutagenesis
has_obo_namespace: eco
id: ECO:0005528
comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site.

Axiom
annotated source: site-directed mutagenesis evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21204030
database_cross_reference: PMID:24011050
database_cross_reference: PMID:3541892

random mutagenesis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis.
created by: snadendla
creation date: 2015-09-30T11:55:03Z
has_obo_namespace: eco
id: ECO:0005529
comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination.

Axiom
annotated source: random mutagenesis evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:15153637
database_cross_reference: PMID:18265275

random mutagenesis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: random mutagenesis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: random mutagenesis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of random mutagenesis evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-09-30T11:57:00Z
has_obo_namespace: eco
id: ECO:0005530

Axiom
annotated source: random mutagenesis evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: random mutagenesis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

motif discovery evidence
type: Class
subclass of: multiple sequence alignment evidence
definition: A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs.
created by: snadendla
creation date: 2015-09-30T15:04:45Z
has_obo_namespace: eco
id: ECO:0005531
comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF (PMID: 19892760), from peaks in ChIP-seq experiments with the TF (PMID: 16264194), or from the comparative genomics analysis of the promoter regions of orthologs (PMID: 23032607).

Axiom
annotated source: motif discovery evidence
annotated property: definition
annotated target: A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10812473
database_cross_reference: PMID:16845028
database_cross_reference: PMID:8211139

consensus search evidence
type: Class
subclass of: motif similarity evidence
definition: A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match.
created by: snadendla
creation date: 2015-10-09T14:12:54Z
has_obo_namespace: eco
id: ECO:0005532
comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555.

Axiom
annotated source: consensus search evidence
annotated property: definition
annotated target: A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match.
database_cross_reference: ECO:SW
database_cross_reference: PMID:15130839

phylogenetic footprinting evidence
type: Class
subclass of: motif discovery evidence
definition: A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'.
created by: snadendla
creation date: 2015-09-30T16:23:02Z
has_obo_namespace: eco
id: ECO:0005533
comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340).

Axiom
annotated source: phylogenetic footprinting evidence
annotated property: definition
annotated target: A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'.
database_cross_reference: ECO:SW
database_cross_reference: PMID:16477324
database_cross_reference: PMID:3199442

comparative genomics motif search evidence
type: Class
subclass of: motif similarity evidence
definition: A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes.
created by: snadendla
creation date: 2015-10-09T14:30:38Z
has_obo_namespace: eco
id: ECO:0005534
comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used.

Axiom
annotated source: comparative genomics motif search evidence
annotated property: definition
annotated target: A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10854408

machine learning prediction of motif instance evidence
type: Class
subclass of: match to sequence model evidence
definition: A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences.
created by: snadendla
creation date: 2015-10-01T11:58:36Z
has_obo_namespace: eco
id: ECO:0005535
comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297).

Axiom
annotated source: machine learning prediction of motif instance evidence
annotated property: definition
annotated target: A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17117497
database_cross_reference: PMID:2014171

position-specific scoring matrix motif search evidence
type: Class
subclass of: motif similarity evidence
definition: A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis.
created by: snadendla
creation date: 2015-10-09T15:04:35Z
has_exact_synonym: PSSM motif search
has_narrow_synonym: PSSM
has_obo_namespace: eco
id: ECO:0005536
comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457).

Axiom
annotated source: position-specific scoring matrix motif search evidence
annotated property: definition
annotated target: A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10812473

xylE reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
definition: A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene.
created by: snadendla
creation date: 2015-10-28T15:31:28Z
has_obo_namespace: eco
id: ECO:0005537

Axiom
annotated source: xylE reporter gene assay evidence
annotated property: definition
annotated target: A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene.
database_cross_reference: ECO:SW
database_cross_reference: PMID:2592344

computationally derived logical inference
type: Class
created by: snadendla
creation date: 2015-11-17T15:15:50Z
has_obo_namespace: eco
id: ECO:0005538
deprecated: true

computationally derived logical inference used in automatic assertion
type: Class
created by: snadendla
creation date: 2015-11-17T15:17:47Z
has_obo_namespace: eco
id: ECO:0005539
deprecated: true

computationally derived logical inference from automatic assertion used in automatic assertion
type: Class
created by: snadendla
creation date: 2015-11-17T15:24:42Z
has_obo_namespace: eco
id: ECO:0005540
deprecated: true

computationally derived logical inference from manual assertion used in automatic assertion
type: Class
created by: snadendla
creation date: 2015-11-17T15:32:03Z
has_obo_namespace: eco
id: ECO:0005541
deprecated: true

biological system reconstruction evidence by experimental evidence from single species used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence by experimental evidence from single species
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence by experimental evidence from single species
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion.
created by: snadendla
creation date: 2015-12-16T15:32:41Z
has_obo_namespace: eco
id: ECO:0005542
comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof.

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19450514

biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence by experimental evidence from mixed species
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence by experimental evidence from mixed species
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion.
created by: snadendla
creation date: 2015-12-16T15:45:01Z
has_obo_namespace: eco
id: ECO:0005543
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22232657

biological system reconstruction evidence based on orthology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biological system reconstruction evidence based on orthology evidence
subclass of: biological system reconstruction evidence based on homology evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-12-18T16:20:49Z
has_obo_namespace: eco
id: ECO:0005544
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence based on orthology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on orthology evidence used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

biological system reconstruction evidence based on orthology evidence
type: Class
subclass of: biological system reconstruction evidence based on homology evidence
definition: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species.
created by: snadendla
creation date: 2015-12-16T15:57:37Z
has_obo_namespace: eco
id: ECO:0005545
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence based on orthology evidence
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128

biological system reconstruction evidence based on paralogy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on paralogy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biological system reconstruction evidence based on paralogy evidence
subclass of: biological system reconstruction evidence based on homology evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2015-12-16T16:05:15Z
has_obo_namespace: eco
id: ECO:0005546
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence based on paralogy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on paralogy evidence used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128

biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on inference from background scientific knowledge
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence based on inference from background scientific knowledge
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion.
created by: snadendla
creation date: 2015-12-16T16:07:42Z
has_obo_namespace: eco
id: ECO:0005547
comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components.

Axiom
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17876790

biological system reconstruction evidence based on inference from background scientific knowledge
type: Class
subclass of: biological system reconstruction evidence
definition: A type of biological system reconstruction based solely on background scientific knowledge of that biological system.
created by: snadendla
creation date: 2015-12-18T15:32:15Z
has_obo_namespace: eco
id: ECO:0005548
comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components.

Axiom
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge
annotated property: definition
annotated target: A type of biological system reconstruction based solely on background scientific knowledge of that biological system.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17876790

biological system reconstruction evidence based on homology evidence
type: Class
subclass of: biological system reconstruction evidence
definition: A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex.
created by: snadendla
creation date: 2015-12-18T15:49:46Z
has_obo_namespace: eco
id: ECO:0005549
comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species.

Axiom
annotated source: biological system reconstruction evidence based on homology evidence
annotated property: definition
annotated target: A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128

biological system reconstruction evidence based on paralogy evidence
type: Class
subclass of: biological system reconstruction evidence based on homology evidence
definition: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species.
created by: snadendla
creation date: 2015-12-18T16:24:08Z
has_obo_namespace: eco
id: ECO:0005550
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence based on paralogy evidence
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128

biological system reconstruction evidence by experimental evidence
type: Class
subclass of: biological system reconstruction evidence
definition: A type of biological system reconstruction evidence that uses experimental evidence as support.
created by: snadendla
creation date: 2015-12-18T16:34:13Z
has_obo_namespace: eco
id: ECO:0005551

Axiom
annotated source: biological system reconstruction evidence by experimental evidence
annotated property: definition
annotated target: A type of biological system reconstruction evidence that uses experimental evidence as support.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19450514
database_cross_reference: PMID:22232657

biological system reconstruction evidence by experimental evidence from mixed species
type: Class
subclass of: biological system reconstruction evidence by experimental evidence
definition: A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment.
created by: snadendla
creation date: 2015-12-18T16:35:13Z
has_obo_namespace: eco
id: ECO:0005552
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments.

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from mixed species
annotated property: definition
annotated target: A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22232657

biological system reconstruction evidence by experimental evidence from single species
type: Class
subclass of: biological system reconstruction evidence by experimental evidence
definition: A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species.
created by: snadendla
creation date: 2015-12-18T16:36:44Z
has_obo_namespace: eco
id: ECO:0005553
comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof.

Axiom
annotated source: biological system reconstruction evidence by experimental evidence from single species
annotated property: definition
annotated target: A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species.
database_cross_reference: ECO:SN

pairwise sequence alignment evidence
type: Class
subclass of: sequence alignment evidence
definition: A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).
created by: snadendla
creation date: 2016-02-08T14:30:46Z
has_obo_namespace: eco
id: ECO:0005554

Axiom
annotated source: pairwise sequence alignment evidence
annotated property: definition
annotated target: A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.ebi.ac.uk/Tools/psa/

multiple sequence alignment evidence
type: Class
subclass of: sequence alignment evidence
definition: A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied.
created by: snadendla
creation date: 2016-02-08T14:31:24Z
has_obo_namespace: eco
id: ECO:0005555

Axiom
annotated source: multiple sequence alignment evidence
annotated property: definition
annotated target: A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.ebi.ac.uk/Tools/msa/

multiple sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: multiple sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: multiple sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of multiple sequence alignment evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-08T14:59:18Z
has_obo_namespace: eco
id: ECO:0005556

Axiom
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: definition
annotated target: A type of multiple sequence alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

multiple sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: multiple sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: multiple sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of multiple sequence alignment evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-02-08T15:00:50Z
has_obo_namespace: eco
id: ECO:0005557

Axiom
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: definition
annotated target: A type of multiple sequence alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

motif discovery evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motif discovery evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif discovery evidence
subclass of: multiple sequence alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of motif discovery evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-08T15:07:47Z
has_obo_namespace: eco
id: ECO:0005558

Axiom
annotated source: motif discovery evidence used in manual assertion
annotated property: subClassOf
annotated target: multiple sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: motif discovery evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: motif discovery evidence used in manual assertion
annotated property: definition
annotated target: A type of motif discovery evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

motif discovery evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: motif discovery evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif discovery evidence
subclass of: multiple sequence alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of motif discovery evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-02-08T15:08:19Z
has_obo_namespace: eco
id: ECO:0005559

Axiom
annotated source: motif discovery evidence used in automatic assertion
annotated property: subClassOf
annotated target: multiple sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: motif discovery evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: motif discovery evidence used in automatic assertion
annotated property: definition
annotated target: A type of motif discovery evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

pairwise sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: pairwise sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: pairwise sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of pairwise sequence alignment evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-08T15:17:05Z
has_obo_namespace: eco
id: ECO:0005560

Axiom
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in manual assertion
is inferred: true

Axiom
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: definition
annotated target: A type of pairwise sequence alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

pairwise sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: pairwise sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: pairwise sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of pairwise sequence alignment evidence that is used in an automatic evidence.
created by: snadendla
creation date: 2016-02-08T15:17:52Z
has_obo_namespace: eco
id: ECO:0005561

Axiom
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: sequence alignment evidence used in automatic assertion
is inferred: true

Axiom
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: definition
annotated target: A type of pairwise sequence alignment evidence that is used in an automatic evidence.
database_cross_reference: ECO:SN

in gel protein kinase assay evidence
type: Class
subclass of: in vitro assay evidence
created by: snadendla
creation date: 2016-02-09T12:05:29Z
has_obo_namespace: eco
id: ECO:0005562

macroscopic current trace evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording.
created by: snadendla
creation date: 2016-02-09T12:40:03Z
has_obo_namespace: eco
id: ECO:0005563

Axiom
annotated source: macroscopic current trace evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17938230
database_cross_reference: url:http://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf

current density evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane.
created by: snadendla
creation date: 2016-02-09T12:43:07Z
has_obo_namespace: eco
id: ECO:0005564

Axiom
annotated source: current density evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/102/11/1298.full
database_cross_reference: url:http://www.bem.fi/book/04/04.htm

single channel conductance evidence
type: Class
created by: snadendla
creation date: 2016-02-09T12:45:42Z
has_obo_namespace: eco
id: ECO:0005565
deprecated: true

sustained current evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels.
created by: snadendla
creation date: 2016-02-09T12:46:54Z
has_obo_namespace: eco
id: ECO:0005566

Axiom
annotated source: sustained current evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html

steady state activation curve evidence
type: Class
created by: snadendla
creation date: 2016-02-09T12:48:17Z
has_obo_namespace: eco
id: ECO:0005567
deprecated: true

steady state inactivation curve evidence
type: Class
created by: snadendla
creation date: 2016-02-09T12:50:45Z
has_obo_namespace: eco
id: ECO:0005568
deprecated: true

window current trace evidence
type: Class
created by: snadendla
creation date: 2016-02-09T12:57:16Z
has_obo_namespace: eco
id: ECO:0005569
deprecated: true

use dependence of inactivation evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course.
created by: snadendla
creation date: 2016-02-09T12:58:18Z
has_obo_namespace: eco
id: ECO:0005570

Axiom
annotated source: use dependence of inactivation evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/89/8/700.full

current clamp voltage recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell.
created by: snadendla
creation date: 2016-02-12T13:58:32Z
database_cross_reference: birnlex:2278
has_obo_namespace: eco
id: ECO:0005571

Axiom
annotated source: current clamp voltage recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html

current clamp voltage recording protocol
type: Axiom
annotated source: current clamp voltage recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2278

whole-cell voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage.
created by: snadendla
creation date: 2016-02-12T14:06:40Z
database_cross_reference: birnlex:2281
has_obo_namespace: eco
id: ECO:0005572

Axiom
annotated source: whole-cell voltage clamp recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/72/1/91.full.pdf

whole-cell voltage clamp recording protocol
type: Axiom
annotated source: whole-cell voltage clamp recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2281

cell-attached single-channel recording evidence
type: Class
subclass of: patch-clamp recording evidence
definition: A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction.
created by: snadendla
creation date: 2016-02-12T14:07:39Z
database_cross_reference: birnlex:2283
has_exact_synonym: cell-attached patch-clamp recording evidence
has_obo_namespace: eco
id: ECO:0005573

Axiom
annotated source: cell-attached single-channel recording evidence
annotated property: definition
annotated target: A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf

cell-attached single-channel recording protocol
type: Axiom
annotated source: cell-attached single-channel recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2283

cell-detached inside-out single-channel recording evidence
type: Class
subclass of: patch-clamp recording evidence
definition: A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution.
created by: snadendla
creation date: 2016-02-12T14:16:53Z
database_cross_reference: birnlex:2286
has_exact_synonym: cell-detached inside-out patch-clamp recording evidence
has_obo_namespace: eco
id: ECO:0005574

Axiom
annotated source: cell-detached inside-out single-channel recording evidence
annotated property: definition
annotated target: A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.acnp.org/g4/GN401000005/CH005.html

cell-detached inside-out single-channel recording protocol
type: Axiom
annotated source: cell-detached inside-out single-channel recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2286

reconstituted bilayer single-channel patch recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane.
created by: snadendla
creation date: 2016-02-12T14:17:46Z
database_cross_reference: birnlex:2287
has_obo_namespace: eco
id: ECO:0005575

Axiom
annotated source: reconstituted bilayer single-channel patch recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane.
database_cross_reference: ECO:SN
database_cross_reference: url:http://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf

reconstituted bilayer single-channel patch recording protocol
type: Axiom
annotated source: reconstituted bilayer single-channel patch recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2287

voltage clamp current recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage.
created by: snadendla
creation date: 2016-02-12T14:18:36Z
database_cross_reference: birnlex:2279
has_obo_namespace: eco
id: ECO:0005576

Axiom
annotated source: voltage clamp current recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage.
database_cross_reference: ECO:SN
database_cross_reference: url:http://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf
database_cross_reference: url:http://www.acnp.org/g4/GN401000005/CH005.html

voltage clamp current recording protocol
type: Axiom
annotated source: voltage clamp current recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2279

electroencephalography recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head.
created by: snadendla
creation date: 2016-02-12T14:19:21Z
database_cross_reference: birnlex:2293
has_obo_namespace: eco
id: ECO:0005577

Axiom
annotated source: electroencephalography recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.jove.com/science-education/5420/electro-encephalography-eeg

electroencephalography recording protocol
type: Axiom
annotated source: electroencephalography recording evidence
annotated property: database_cross_reference
annotated target: birnlex:2293

super-resolution microscopy evidence
type: Class
subclass of: microscopy evidence
definition: A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures.
created by: snadendla
creation date: 2016-02-12T15:20:47Z
has_obo_namespace: eco
id: ECO:0005578

Axiom
annotated source: super-resolution microscopy evidence
annotated property: definition
annotated target: A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures.
database_cross_reference: ECO:SN
database_cross_reference: url:http://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full

immunogold labelling evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunogold labelling evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunogold labelling evidence
subclass of: immunolocalization evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunogold labelling evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-12T15:38:03Z
has_obo_namespace: eco
id: ECO:0005579

Axiom
annotated source: immunogold labelling evidence used in manual assertion
annotated property: subClassOf
annotated target: immunolocalization evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunogold labelling evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunogold labelling evidence used in manual assertion
annotated property: definition
annotated target: A type of immunogold labelling evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

flow cytometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: flow cytometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: flow cytometry evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of flow cytometry evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-12T16:04:23Z
has_obo_namespace: eco
id: ECO:0005580

Axiom
annotated source: flow cytometry evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: flow cytometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: flow cytometry evidence used in manual assertion
annotated property: definition
annotated target: A type of flow cytometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

enzyme-linked immunoabsorbent assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: enzyme-linked immunoabsorbent assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: enzyme-linked immunoabsorbent assay evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-02-12T16:23:38Z
has_obo_namespace: eco
id: ECO:0005581

Axiom
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: definition
annotated target: A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

cell-detached outside-out single-channel recording evidence
type: Class
subclass of: patch-clamp recording evidence
definition: A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch).
created by: snadendla
creation date: 2016-03-02T10:47:25Z
has_exact_synonym: cell-detached outside-out patch-clamp evidence
has_obo_namespace: eco
id: ECO:0005582

Axiom
annotated source: cell-detached outside-out single-channel recording evidence
annotated property: definition
annotated target: A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch).
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html

cut-open oocyte voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway.
created by: snadendla
creation date: 2016-03-02T11:29:32Z
has_obo_namespace: eco
id: ECO:0005583

Axiom
annotated source: cut-open oocyte voltage clamp recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway.
database_cross_reference: ECO:SN
database_cross_reference: PMID:9711615

macropatch voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch).
created by: snadendla
creation date: 2016-03-02T11:33:48Z
has_obo_namespace: eco
id: ECO:0005584

Axiom
annotated source: macropatch voltage clamp recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch).
database_cross_reference: ECO:SN
database_cross_reference: url:http://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf

high throughput mass spectrometry evidence
type: Class
subclass of: mass spectrometry evidence
definition: A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies.
created by: snadendla
creation date: 2016-03-02T12:33:07Z
has_obo_namespace: eco
id: ECO:0005585
comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec. For HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead.

Axiom
annotated source: high throughput mass spectrometry evidence
annotated property: definition
annotated target: A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies.
database_cross_reference: ECO:SN
database_cross_reference: PMID:20805795

high throughput mass spectrometry evidence used in manual assertion
type: Class
subclass of: high throughput mass spectrometry evidence
definition: A type of high throughput mass spectrometry evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-02T12:50:02Z
has_obo_namespace: eco
id: ECO:0005586

Axiom
annotated source: high throughput mass spectrometry evidence used in manual assertion
annotated property: definition
annotated target: A type of high throughput mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

confocal microscopy evidence
type: Class
subclass of: microscopy evidence
definition: A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis.
created by: snadendla
creation date: 2016-03-17T12:29:45Z
has_obo_namespace: eco
id: ECO:0005587

Axiom
annotated source: confocal microscopy evidence
annotated property: definition
annotated target: A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf

wide-field microscopy evidence
type: Class
subclass of: microscopy evidence
definition: A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times.
created by: snadendla
creation date: 2016-03-17T12:30:27Z
has_obo_namespace: eco
id: ECO:0005588

Axiom
annotated source: wide-field microscopy evidence
annotated property: definition
annotated target: A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times.
database_cross_reference: ECO:SN
database_cross_reference: url:http://jcb.rupress.org/content/172/1/9.full
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/
database_cross_reference: url:http://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html

confocal microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: confocal microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: confocal microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type confocal microscopy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-17T12:32:24Z
has_obo_namespace: eco
id: ECO:0005589

Axiom
annotated source: confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: confocal microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type confocal microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

wide-field microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: wide-field microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: wide-field microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of wide-field microscopy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-17T12:34:42Z
has_obo_namespace: eco
id: ECO:0005590

Axiom
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of wide-field microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

immunogold labelling electron microscopy assay evidence
type: Class
subclass of: immunogold labelling evidence
subclass of: electron microscopy evidence
definition: A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles.
created by: snadendla
creation date: 2016-03-17T15:43:00Z
has_obo_namespace: eco
id: ECO:0005591

Axiom
annotated source: immunogold labelling electron microscopy assay evidence
annotated property: definition
annotated target: A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles.
database_cross_reference: ECO:SN
database_cross_reference: PMID:4110101

immunogold labelling electron microscopy assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunogold labelling electron microscopy assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunogold labelling evidence used in manual assertion
subclass of: immunogold labelling electron microscopy assay evidence
subclass of: electron microscopy evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-17T16:39:37Z
has_obo_namespace: eco
id: ECO:0005592

Axiom
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: subClassOf
annotated target: immunogold labelling evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: subClassOf
annotated target: electron microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: definition
annotated target: A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

immunodetection assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence that involves the use of antibodies to detect biomolecules.
created by: snadendla
creation date: 2016-03-17T17:07:33Z
has_obo_namespace: eco
id: ECO:0005593

Axiom
annotated source: immunodetection assay evidence
annotated property: definition
annotated target: A type of direct assay evidence that involves the use of antibodies to detect biomolecules.
database_cross_reference: ECO:SN

immunoperoxidase immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
definition: A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody.
created by: snadendla
creation date: 2016-03-17T17:11:34Z
has_obo_namespace: eco
id: ECO:0005594

Axiom
annotated source: immunoperoxidase immunolocalization evidence
annotated property: definition
annotated target: A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody.
database_cross_reference: ECO:SN

immunoperoxidase immunolocalization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunoperoxidase immunolocalization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunoperoxidase immunolocalization evidence
subclass of: immunolocalization evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-17T17:14:05Z
has_obo_namespace: eco
id: ECO:0005595

Axiom
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: subClassOf
annotated target: immunolocalization evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: definition
annotated target: A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

immunoperoxidase immunolocalization electron microscopy evidence
type: Class
subclass of: electron microscopy evidence
subclass of: immunoperoxidase immunolocalization evidence
definition: A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody.
created by: snadendla
creation date: 2016-03-17T17:16:38Z
has_exact_synonym: Immunoperoxidase labelling electron microscopy
has_obo_namespace: eco
id: ECO:0005596

Axiom
annotated source: immunoperoxidase immunolocalization electron microscopy evidence
annotated property: definition
annotated target: A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody.
database_cross_reference: ECO:SN

immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunoperoxidase immunolocalization electron microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunoperoxidase immunolocalization evidence used in manual assertion
subclass of: immunoperoxidase immunolocalization electron microscopy evidence
subclass of: electron microscopy evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-17T17:23:56Z
has_obo_namespace: eco
id: ECO:0005597

Axiom
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: immunoperoxidase immunolocalization evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: electron microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

wide-field fluorescence microscopy evidence
type: Class
subclass of: wide-field microscopy evidence
definition: A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter.
created by: snadendla
creation date: 2016-03-18T11:53:56Z
has_obo_namespace: eco
id: ECO:0005598
comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained.

Axiom
annotated source: wide-field fluorescence microscopy evidence
annotated property: definition
annotated target: A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.bristol.ac.uk/synaptic/research/techniques/widefield.html

immunofluorescence wide-field microscopy evidence
type: Class
subclass of: immunofluorescence evidence
subclass of: wide-field fluorescence microscopy evidence
definition: A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens.
created by: snadendla
creation date: 2016-03-18T11:55:40Z
has_exact_synonym: wide-field epifluorescence microscopy
has_obo_namespace: eco
id: ECO:0005599

Axiom
annotated source: immunofluorescence wide-field microscopy evidence
annotated property: definition
annotated target: A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens.
database_cross_reference: ECO:SN
database_cross_reference: url:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135

immunofluorescence confocal microscopy evidence
type: Class
subclass of: immunofluorescence evidence
subclass of: confocal microscopy evidence
definition: A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection.
created by: snadendla
creation date: 2016-03-18T12:48:42Z
has_obo_namespace: eco
id: ECO:0005600
comment: This allows visualization of sub cellular distribution of biomolecules of interest.

Axiom
annotated source: immunofluorescence confocal microscopy evidence
annotated property: definition
annotated target: A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.physics.emory.edu/faculty/weeks/confocal/
database_cross_reference: url:https://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf

immunofluorescence confocal microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunofluorescence confocal microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: confocal microscopy evidence used in manual assertion
subclass of: immunofluorescence confocal microscopy evidence
subclass of: immunofluorescence evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-18T12:52:04Z
has_obo_namespace: eco
id: ECO:0005601

Axiom
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: confocal microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: immunofluorescence evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

protein expression level evidence based on western blot
type: Class
subclass of: protein expression level evidence
subclass of: western blot evidence
definition: A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique.
created by: snadendla
creation date: 2016-03-18T13:02:29Z
has_obo_namespace: eco
id: ECO:0005602

Axiom
annotated source: protein expression level evidence based on western blot
annotated property: definition
annotated target: A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique.
database_cross_reference: ECO:SN

protein mass spectrometry evidence
type: Class
subclass of: protein assay evidence
subclass of: mass spectrometry evidence
definition: A type of mass spectrometry evidence where one or more protein analyses are performed.
created by: snadendla
creation date: 2016-03-18T13:11:37Z
has_obo_namespace: eco
id: ECO:0005603

Axiom
annotated source: protein mass spectrometry evidence
annotated property: definition
annotated target: A type of mass spectrometry evidence where one or more protein analyses are performed.
database_cross_reference: ECO:SN

cross-streak test evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics.
created by: snadendla
creation date: 2016-03-29T15:40:54Z
has_obo_namespace: eco
id: ECO:0005604

Axiom
annotated source: cross-streak test evidence
annotated property: definition
annotated target: A type of experimental evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics.
database_cross_reference: ECO:SN
database_cross_reference: url:www.jstor.org/stable/3792785

disk diffusion test evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism.
created by: snadendla
creation date: 2016-03-29T16:02:22Z
has_exact_synonym: zone of inhibition evidence
has_obo_namespace: eco
id: ECO:0005605

Axiom
annotated source: disk diffusion test evidence
annotated property: definition
annotated target: A type of experimental evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.cdc.gov/nczved/resources/cholera/ch9.pdf

cell transfection experiment evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function.
created by: snadendla
creation date: 2016-03-29T16:59:17Z
has_obo_namespace: eco
id: ECO:0005606

Axiom
annotated source: cell transfection experiment evidence
annotated property: definition
annotated target: A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function.
database_cross_reference: ECO:SN
database_cross_reference: url:http://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/

tethered cell assay evidence
type: Class
subclass of: chemotaxis assay evidence
definition: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by observing the rotational behavior of the organism tethered to a coverslip by their flagella.
created by: snadendla
creation date: 2016-03-31T15:51:10Z
has_obo_namespace: eco
id: ECO:0005607

Axiom
annotated source: tethered cell assay evidence
annotated property: definition
annotated target: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by observing the rotational behavior of the organism tethered to a coverslip by their flagella.
database_cross_reference: ECO:SN
database_cross_reference: PMID:1103143

tumble frequency assay evidence
type: Class
subclass of: chemotaxis assay evidence
definition: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another.
created by: snadendla
creation date: 2016-03-31T16:42:35Z
has_exact_synonym: Temporal assay
has_obo_namespace: eco
id: ECO:0005608

Axiom
annotated source: tumble frequency assay evidence
annotated property: definition
annotated target: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another.
database_cross_reference: ECO:SN
database_cross_reference: PMID:4576832

capillary assay evidence
type: Class
subclass of: chemotaxis assay evidence
definition: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined (typically bacteria or archaea) by measuring the rate of accumulation of the organism into a capillary.
created by: snadendla
creation date: 2016-03-31T16:45:29Z
has_obo_namespace: eco
id: ECO:0005609
comment: To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary. To determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured.

Axiom
annotated source: capillary assay evidence
annotated property: definition
annotated target: A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined (typically bacteria or archaea) by measuring the rate of accumulation of the organism into a capillary.
database_cross_reference: ECO:SN
database_cross_reference: PMID:4632978

biological system reconstruction evidence based on homology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on homology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence based on homology evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-03-31T17:00:03Z
has_obo_namespace: eco
id: ECO:0005610
comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species.

Axiom
annotated source: biological system reconstruction evidence based on homology evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on homology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: biological system reconstruction evidence based on homology evidence used in manual assertion
annotated property: definition
annotated target: A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

inference from experimental data evidence
type: Class
subclass of: direct assay evidence used in manual assertion
definition: A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease.
created by: snadendla
creation date: 2016-04-12T16:55:16Z
has_exact_synonym: IED
has_exact_synonym: inferred from experimental data
has_obo_namespace: eco
id: ECO:0005611

Axiom
annotated source: inference from experimental data evidence
annotated property: definition
annotated target: A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease.
database_cross_reference: ECO:SN

inference from phenotype manipulation evidence
type: Class
subclass of: mutant phenotype evidence used in manual assertion
definition: A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined.
created by: snadendla
creation date: 2016-04-12T17:09:43Z
has_exact_synonym: IPM
has_exact_synonym: inferred from phenotype manipulation
has_obo_namespace: eco
id: ECO:0005612
comment: Changes can include any gene mutation/knockout, over-expression or ectopic expression of wild-type or mutant genes, anti-sense experiments, RNAi experiments, specific protein inhibitors.

Axiom
annotated source: inference from phenotype manipulation evidence
annotated property: definition
annotated target: A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined.
database_cross_reference: ECO:SN

inference by association of genotype from phenotype
type: Class
subclass of: natural variation mutant evidence used in manual assertion
definition: A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments.
created by: snadendla
creation date: 2016-04-12T17:24:00Z
has_exact_synonym: IAGP
has_exact_synonym: inferred by association of genotype from phenotype
has_obo_namespace: eco
id: ECO:0005613
comment: Used for 1) polymorphism or segregation of genetic markers (SNPs, mutations, RFLPs, microsatellites); 2) polymorphism or segregation of physical markers (FISH, centromeric or heterochromatic regions, chromosomal banding patterns); 3) detection of polymorphisms in inbred stock.

Axiom
annotated source: inference by association of genotype from phenotype
annotated property: definition
annotated target: A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments.
database_cross_reference: ECO:SN

two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: two-dimensional polyacrylamide gel electrophoresis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: two-dimensional polyacrylamide gel electrophoresis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T11:03:08Z
has_obo_namespace: eco
id: ECO:0005614

Axiom
annotated source: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion
annotated property: definition
annotated target: A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

alkaline phosphatase reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: alkaline phosphatase reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: alkaline phosphatase reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T11:05:59Z
has_obo_namespace: eco
id: ECO:0005615

Axiom
annotated source: alkaline phosphatase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: alkaline phosphatase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: alkaline phosphatase reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

beta-galactosidase reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: beta-galactosidase reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: beta-galactosidase reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T11:08:57Z
has_obo_namespace: eco
id: ECO:0005616

Axiom
annotated source: beta-galactosidase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: beta-galactosidase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: beta-galactosidase reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chloramphenicol acetyltransferase reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: chloramphenicol acetyltransferase reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T11:11:07Z
has_obo_namespace: eco
id: ECO:0005617

Axiom
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

chromatin immunoprecipitation-chip evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation-chip evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: chromatin immunoprecipitation-chip evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T11:17:04Z
has_obo_namespace: eco
id: ECO:0005618

Axiom
annotated source: chromatin immunoprecipitation-chip evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-chip evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-chip evidence used in automatic assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

chromatin immunoprecipitation- exonuclease evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation- exonuclease evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: chromatin immunoprecipitation- exonuclease evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T11:22:25Z
has_obo_namespace: eco
id: ECO:0005619

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

chromatin immunoprecipitation-PCR evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation-PCR evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: chromatin immunoprecipitation-PCR evidence
subclass of: immunoprecipitation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T11:25:01Z
has_obo_namespace: eco
id: ECO:0005620

Axiom
annotated source: chromatin immunoprecipitation-PCR evidence used in manual assertion
annotated property: subClassOf
annotated target: immunoprecipitation evidence used in manual assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-PCR evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-PCR evidence used in manual assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

chromatin immunoprecipitation-seq evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation-seq evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: chromatin immunoprecipitation-seq evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T11:29:13Z
has_obo_namespace: eco
id: ECO:0005621

Axiom
annotated source: chromatin immunoprecipitation-seq evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-seq evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: chromatin immunoprecipitation-seq evidence used in automatic assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

comparative genomics motif search evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: comparative genomics motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence used in manual assertion
subclass of: comparative genomics motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of comparative genomics motif search evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T14:49:36Z
has_obo_namespace: eco
id: ECO:0005622

Axiom
annotated source: comparative genomics motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: comparative genomics motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: comparative genomics motif search evidence used in manual assertion
annotated property: definition
annotated target: A type of comparative genomics motif search evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

comparative genomics motif search evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: comparative genomics motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence used in automatic assertion
subclass of: comparative genomics motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of comparative genomics motif search evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T14:52:21Z
has_obo_namespace: eco
id: ECO:0005623

Axiom
annotated source: comparative genomics motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: comparative genomics motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: comparative genomics motif search evidence used in automatic assertion
annotated property: definition
annotated target: A type of comparative genomics motif search evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

consensus search evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: consensus search evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence used in manual assertion
subclass of: consensus search evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of consensus search evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T14:54:53Z
has_obo_namespace: eco
id: ECO:0005624

Axiom
annotated source: consensus search evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: consensus search evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: consensus search evidence used in manual assertion
annotated property: definition
annotated target: A type of consensus search evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

consensus search evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: consensus search evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence used in automatic assertion
subclass of: consensus search evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of consensus search evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T14:58:22Z
has_obo_namespace: eco
id: ECO:0005625

Axiom
annotated source: consensus search evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: consensus search evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: consensus search evidence used in automatic assertion
annotated property: definition
annotated target: A type of consensus search evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

copper-phenanthroline footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: copper-phenanthroline footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: copper-phenanthroline footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of copper-phenanthroline footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:01:25Z
has_obo_namespace: eco
id: ECO:0005626

Axiom
annotated source: copper-phenanthroline footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: copper-phenanthroline footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: copper-phenanthroline footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of copper-phenanthroline footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

DNA adenine methyltransferase identification evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA adenine methyltransferase identification evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: DNA adenine methyltransferase identification evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:05:14Z
has_obo_namespace: eco
id: ECO:0005627

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in manual assertion
annotated property: definition
annotated target: A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

DNA adenine methyltransferase identification evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: DNA adenine methyltransferase identification evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: DNA adenine methyltransferase identification evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T15:06:46Z
has_obo_namespace: eco
id: ECO:0005628

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: DNA adenine methyltransferase identification evidence used in automatic assertion
annotated property: definition
annotated target: A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

DNA affinity chromatography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA affinity chromatography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: DNA affinity chromatography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNA affinity chromatography evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:08:40Z
has_obo_namespace: eco
id: ECO:0005629

Axiom
annotated source: DNA affinity chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNA affinity chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNA affinity chromatography evidence used in manual assertion
annotated property: definition
annotated target: A type of DNA affinity chromatography evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

DNA microarray evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: DNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: DNA microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of DNA microarray evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T15:31:51Z
has_obo_namespace: eco
id: ECO:0005630

Axiom
annotated source: DNA microarray evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: DNA microarray evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: DNA microarray evidence used in automatic assertion
annotated property: definition
annotated target: A type of DNA microarray evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

DNAse footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNAse footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: DNAse footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of DNAse footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:35:41Z
has_obo_namespace: eco
id: ECO:0005631

Axiom
annotated source: DNAse footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: DNAse footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: DNAse footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of DNAse footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

fluorescence anisotropy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence anisotropy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: fluorescence evidence used in manual assertion
subclass of: fluorescence anisotropy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of fluorescence anisotropy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:45:40Z
has_obo_namespace: eco
id: ECO:0005632

Axiom
annotated source: fluorescence anisotropy evidence used in manual assertion
annotated property: subClassOf
annotated target: fluorescence evidence used in manual assertion
is inferred: true

Axiom
annotated source: fluorescence anisotropy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: fluorescence anisotropy evidence used in manual assertion
annotated property: definition
annotated target: A type of fluorescence anisotropy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

ferric uptake regulator titration assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ferric uptake regulator titration assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: ferric uptake regulator titration assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of ferric uptake regulator titration assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T15:49:18Z
has_obo_namespace: eco
id: ECO:0005633

Axiom
annotated source: ferric uptake regulator titration assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: ferric uptake regulator titration assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: ferric uptake regulator titration assay evidence used in manual assertion
annotated property: definition
annotated target: A type of ferric uptake regulator titration assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genomic systematic evolution of ligands by exponential amplification evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: genomic systematic evolution of ligands by exponential amplification evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T16:41:30Z
has_obo_namespace: eco
id: ECO:0005634

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion
annotated property: definition
annotated target: A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: genomic systematic evolution of ligands by exponential amplification evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: genomic systematic evolution of ligands by exponential amplification evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T16:43:03Z
has_obo_namespace: eco
id: ECO:0005635

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion
annotated property: definition
annotated target: A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

green fluorescent protein reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: green fluorescent protein reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: green fluorescent protein reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T16:46:32Z
has_obo_namespace: eco
id: ECO:0005636

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

green fluorescent protein reporter gene assay evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: green fluorescent protein reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: green fluorescent protein reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T16:48:29Z
has_obo_namespace: eco
id: ECO:0005637

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: green fluorescent protein reporter gene assay evidence used in automatic assertion
annotated property: definition
annotated target: A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

cell growth assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell growth assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: cell growth assay evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cell growth assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T16:52:15Z
has_obo_namespace: eco
id: ECO:0005638

Axiom
annotated source: cell growth assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: cell growth assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: cell growth assay evidence used in manual assertion
annotated property: definition
annotated target: A type of cell growth assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

cell growth assay evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: cell growth assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: cell growth assay evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of cell growth assay evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T16:53:28Z
has_obo_namespace: eco
id: ECO:0005639

Axiom
annotated source: cell growth assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: cell growth assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: cell growth assay evidence used in automatic assertion
annotated property: definition
annotated target: A type of cell growth assay evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

glutathione S-transferase pull-down assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: glutathione S-transferase pull-down assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: glutathione S-transferase pull-down assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T16:56:39Z
has_obo_namespace: eco
id: ECO:0005640

Axiom
annotated source: glutathione S-transferase pull-down assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: glutathione S-transferase pull-down assay evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: glutathione S-transferase pull-down assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: glutathione S-transferase pull-down assay evidence used in manual assertion
annotated property: definition
annotated target: A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

beta-glucuronidase reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: beta-glucuronidase reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: beta-glucuronidase reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T16:58:29Z
has_obo_namespace: eco
id: ECO:0005641

Axiom
annotated source: beta-glucuronidase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: beta-glucuronidase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: beta-glucuronidase reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

heteronuclear single quantum coherence spectroscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: heteronuclear single quantum coherence spectroscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: nuclear magnetic resonance spectroscopy evidence used in manual assertion
subclass of: heteronuclear single quantum coherence spectroscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:10:13Z
has_obo_namespace: eco
id: ECO:0005642

Axiom
annotated source: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: nuclear magnetic resonance spectroscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

hydroxyl-radical footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: hydroxyl-radical footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: hydroxyl-radical footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of hydroxyl-radical footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:14:48Z
has_obo_namespace: eco
id: ECO:0005643

Axiom
annotated source: hydroxyl-radical footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: hydroxyl-radical footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: hydroxyl-radical footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of hydroxyl-radical footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

immunoprecipitation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunoprecipitation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunoprecipitation evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunoprecipitation evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:20:49Z
has_obo_namespace: eco
id: ECO:0005644

Axiom
annotated source: immunoprecipitation evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunoprecipitation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: immunoprecipitation evidence used in manual assertion
annotated property: definition
annotated target: A type of immunoprecipitation evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

interferometric reflectance imaging sensor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: interferometric reflectance imaging sensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: interferometric reflectance imaging sensor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:27:58Z
has_obo_namespace: eco
id: ECO:0005645

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in manual assertion
annotated property: definition
annotated target: A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

interferometric reflectance imaging sensor evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: interferometric reflectance imaging sensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: interferometric reflectance imaging sensor evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T23:29:23Z
has_obo_namespace: eco
id: ECO:0005646

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: interferometric reflectance imaging sensor evidence used in automatic assertion
annotated property: definition
annotated target: A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

isothermal titration calorimetry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: isothermal titration calorimetry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: isothermal titration calorimetry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of isothermal titration calorimetry evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:36:26Z
has_obo_namespace: eco
id: ECO:0005647

Axiom
annotated source: isothermal titration calorimetry evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: isothermal titration calorimetry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: isothermal titration calorimetry evidence used in manual assertion
annotated property: definition
annotated target: A type of isothermal titration calorimetry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

luciferase reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: luciferase reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: luciferase reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of luciferase reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:44:52Z
has_obo_namespace: eco
id: ECO:0005648

Axiom
annotated source: luciferase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: luciferase reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: luciferase reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of luciferase reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

machine learning prediction of motif instance evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: machine learning prediction of motif instance evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: match to sequence model evidence used in manual assertion
subclass of: machine learning prediction of motif instance evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of machine learning prediction of motif instance evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-06T23:51:30Z
has_obo_namespace: eco
id: ECO:0005649

Axiom
annotated source: machine learning prediction of motif instance evidence used in manual assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in manual assertion
is inferred: true

Axiom
annotated source: machine learning prediction of motif instance evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: machine learning prediction of motif instance evidence used in manual assertion
annotated property: definition
annotated target: A type of machine learning prediction of motif instance evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

machine learning prediction of motif instance evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: machine learning prediction of motif instance evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: match to sequence model evidence used in automatic assertion
subclass of: machine learning prediction of motif instance evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of machine learning prediction of motif instance evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T23:54:00Z
has_obo_namespace: eco
id: ECO:0005650

Axiom
annotated source: machine learning prediction of motif instance evidence used in automatic assertion
annotated property: subClassOf
annotated target: match to sequence model evidence used in automatic assertion
is inferred: true

Axiom
annotated source: machine learning prediction of motif instance evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: machine learning prediction of motif instance evidence used in automatic assertion
annotated property: definition
annotated target: A type of machine learning prediction of motif instance evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-06T23:57:30Z
has_obo_namespace: eco
id: ECO:0005651

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion
annotated property: definition
annotated target: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T14:42:28Z
has_obo_namespace: eco
id: ECO:0005652

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

northern assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: northern assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: northern assay evidence
subclass of: transcript expression level evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of northern assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T14:48:03Z
has_obo_namespace: eco
id: ECO:0005653

Axiom
annotated source: northern assay evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression level evidence used in manual assertion
is inferred: true

Axiom
annotated source: northern assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: northern assay evidence used in manual assertion
annotated property: definition
annotated target: A type of northern assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

phylogenetic footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phylogenetic footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: phylogenetic footprinting evidence
subclass of: motif discovery evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of phylogenetic footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T14:50:52Z
has_obo_namespace: eco
id: ECO:0005654

Axiom
annotated source: phylogenetic footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: motif discovery evidence used in manual assertion
is inferred: true

Axiom
annotated source: phylogenetic footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: phylogenetic footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of phylogenetic footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

phylogenetic footprinting evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: phylogenetic footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: phylogenetic footprinting evidence
subclass of: motif discovery evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of phylogenetic footprinting evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-09T14:51:55Z
has_obo_namespace: eco
id: ECO:0005655

Axiom
annotated source: phylogenetic footprinting evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif discovery evidence used in automatic assertion
is inferred: true

Axiom
annotated source: phylogenetic footprinting evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: phylogenetic footprinting evidence used in automatic assertion
annotated property: definition
annotated target: A type of phylogenetic footprinting evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

premethylation interference footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: premethylation interference footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: premethylation interference footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of premethylation interference footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T14:55:25Z
has_obo_namespace: eco
id: ECO:0005656

Axiom
annotated source: premethylation interference footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: premethylation interference footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: premethylation interference footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of premethylation interference footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

primer extension assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: primer extension assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression level evidence used in manual assertion
subclass of: primer extension assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of primer extension assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T14:58:38Z
has_obo_namespace: eco
id: ECO:0005657

Axiom
annotated source: primer extension assay evidence used in manual assertion
annotated property: subClassOf
annotated target: transcript expression level evidence used in manual assertion
is inferred: true

Axiom
annotated source: primer extension assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: primer extension assay evidence used in manual assertion
annotated property: definition
annotated target: A type of primer extension assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

position-specific scoring matrix motif search evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: position-specific scoring matrix motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence used in manual assertion
subclass of: position-specific scoring matrix motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of position-specific scoring matrix motif search evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T15:01:31Z
has_obo_namespace: eco
id: ECO:0005658

Axiom
annotated source: position-specific scoring matrix motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: position-specific scoring matrix motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: position-specific scoring matrix motif search evidence used in manual assertion
annotated property: definition
annotated target: A type of position-specific scoring matrix motif search evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

position-specific scoring matrix motif search evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: position-specific scoring matrix motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence used in automatic assertion
subclass of: position-specific scoring matrix motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-09T15:02:31Z
has_obo_namespace: eco
id: ECO:0005659

Axiom
annotated source: position-specific scoring matrix motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: position-specific scoring matrix motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: position-specific scoring matrix motif search evidence used in automatic assertion
annotated property: definition
annotated target: A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

quantitative polymerase chain reaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: quantitative polymerase chain reaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: quantitative polymerase chain reaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of quantitative polymerase chain reaction evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T15:09:01Z
has_obo_namespace: eco
id: ECO:0005660

Axiom
annotated source: quantitative polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: quantitative polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: quantitative polymerase chain reaction evidence used in manual assertion
annotated property: definition
annotated target: A type of quantitative polymerase chain reaction evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: rapid amplification of cDNA ends polymerase chain reaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: rapid amplification of cDNA ends polymerase chain reaction evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T15:19:20Z
has_obo_namespace: eco
id: ECO:0005661

Axiom
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion
annotated property: definition
annotated target: A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

regular expression motif search evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: regular expression motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence used in manual assertion
subclass of: regular expression motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of regular expression motif search evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:13:55Z
has_obo_namespace: eco
id: ECO:0005662

Axiom
annotated source: regular expression motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in manual assertion
is inferred: true

Axiom
annotated source: regular expression motif search evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: regular expression motif search evidence used in manual assertion
annotated property: definition
annotated target: A type of regular expression motif search evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

regular expression motif search evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: regular expression motif search evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence used in automatic assertion
subclass of: regular expression motif search evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of regular expression motif search evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-09T16:15:03Z
has_obo_namespace: eco
id: ECO:0005663

Axiom
annotated source: regular expression motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: motif similarity evidence used in automatic assertion
is inferred: true

Axiom
annotated source: regular expression motif search evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: regular expression motif search evidence used in automatic assertion
annotated property: definition
annotated target: A type of regular expression motif search evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

RNA sequencing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA sequencing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: RNA sequencing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of RNA sequencing assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:18:35Z
has_obo_namespace: eco
id: ECO:0005664

Axiom
annotated source: RNA sequencing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: RNA sequencing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: RNA sequencing assay evidence used in manual assertion
annotated property: definition
annotated target: A type of RNA sequencing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

RNA sequencing assay evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: RNA sequencing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: RNA sequencing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of RNA sequencing assay evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-09T16:19:38Z
has_obo_namespace: eco
id: ECO:0005665

Axiom
annotated source: RNA sequencing assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: RNA sequencing assay evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: RNA sequencing assay evidence used in automatic assertion
annotated property: definition
annotated target: A type of RNA sequencing assay evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

S1 nuclease protection assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: S1 nuclease protection assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: nuclease protection assay evidence used in manual assertion
subclass of: S1 nuclease protection assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of S1 nuclease protection assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:22:58Z
has_obo_namespace: eco
id: ECO:0005666

Axiom
annotated source: S1 nuclease protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: nuclease protection assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: S1 nuclease protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: S1 nuclease protection assay evidence used in manual assertion
annotated property: definition
annotated target: A type of S1 nuclease protection assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

site-directed mutagenesis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: site-directed mutagenesis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: site-directed mutagenesis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of site-directed mutagenesis evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:29:13Z
has_obo_namespace: eco
id: ECO:0005667

Axiom
annotated source: site-directed mutagenesis evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: site-directed mutagenesis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: site-directed mutagenesis evidence used in manual assertion
annotated property: definition
annotated target: A type of site-directed mutagenesis evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

survival rate analysis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: survival rate analysis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: knockout evidence used in manual assertion
subclass of: survival rate analysis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of survival rate analysis evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:31:08Z
has_obo_namespace: eco
id: ECO:0005668

Axiom
annotated source: survival rate analysis evidence used in manual assertion
annotated property: subClassOf
annotated target: knockout evidence used in manual assertion
is inferred: true

Axiom
annotated source: survival rate analysis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: survival rate analysis evidence used in manual assertion
annotated property: definition
annotated target: A type of survival rate analysis evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

ultraviolet light footprinting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ultraviolet light footprinting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: ultraviolet light footprinting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of ultraviolet light footprinting evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-09T16:33:44Z
has_obo_namespace: eco
id: ECO:0005669

Axiom
annotated source: ultraviolet light footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: ultraviolet light footprinting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: ultraviolet light footprinting evidence used in manual assertion
annotated property: definition
annotated target: A type of ultraviolet light footprinting evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

x-ray crystallography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: x-ray crystallography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: crystallography evidence used in manual assertion
subclass of: x-ray crystallography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of x-ray crystallography evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-10T11:53:00Z
has_obo_namespace: eco
id: ECO:0005670

Axiom
annotated source: x-ray crystallography evidence used in manual assertion
annotated property: subClassOf
annotated target: crystallography evidence used in manual assertion
is inferred: true

Axiom
annotated source: x-ray crystallography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: x-ray crystallography evidence used in manual assertion
annotated property: definition
annotated target: A type of x-ray crystallography evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

x-ray crystallography evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: x-ray crystallography evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: x-ray crystallography evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of x-ray crystallography evidence that is used in an automatic assertion.
created by: snadendla
creation date: 2016-05-10T11:54:03Z
has_obo_namespace: eco
id: ECO:0005671

Axiom
annotated source: x-ray crystallography evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: x-ray crystallography evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion
is inferred: true

Axiom
annotated source: x-ray crystallography evidence used in automatic assertion
annotated property: definition
annotated target: A type of x-ray crystallography evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN

xylE reporter gene assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: xylE reporter gene assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: xylE reporter gene assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of xylE reporter gene assay evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-10T11:57:15Z
has_obo_namespace: eco
id: ECO:0005672

Axiom
annotated source: xylE reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: xylE reporter gene assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: xylE reporter gene assay evidence used in manual assertion
annotated property: definition
annotated target: A type of xylE reporter gene assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

ad-hoc qualitative phenotype observation evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor.
created by: snadendla
creation date: 2016-05-10T12:57:41Z
has_obo_namespace: eco
id: ECO:0005673

Axiom
annotated source: ad-hoc qualitative phenotype observation evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15150239

ad-hoc qualitative phenotype observation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ad-hoc qualitative phenotype observation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: ad-hoc qualitative phenotype observation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of ad-hoc qualitative phenotype observation that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-10T14:28:47Z
has_obo_namespace: eco
id: ECO:0005674

Axiom
annotated source: ad-hoc qualitative phenotype observation evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: ad-hoc qualitative phenotype observation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: ad-hoc qualitative phenotype observation evidence used in manual assertion
annotated property: definition
annotated target: A type of ad-hoc qualitative phenotype observation that is used in a manual assertion.
database_cross_reference: ECO:SN

ad-hoc quantitative phenotype observation evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor.
created by: snadendla
creation date: 2016-05-10T14:30:50Z
has_obo_namespace: eco
id: ECO:0005675

Axiom
annotated source: ad-hoc quantitative phenotype observation evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15150239

ad-hoc quantitative phenotype observation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ad-hoc quantitative phenotype observation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: ad-hoc quantitative phenotype observation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion.
created by: snadendla
creation date: 2016-05-10T14:34:50Z
has_obo_namespace: eco
id: ECO:0005676

Axiom
annotated source: ad-hoc quantitative phenotype observation evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: ad-hoc quantitative phenotype observation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion
is inferred: true

Axiom
annotated source: ad-hoc quantitative phenotype observation evidence used in manual assertion
annotated property: definition
annotated target: A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

dilution assay evidence
type: Class
subclass of: direct assay evidence
definition: A direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response.
has_exact_synonym: critical dilution assay
has_exact_synonym: spot assay
comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay.

Axiom
annotated source: dilution assay evidence
annotated property: definition
annotated target: A direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response.
database_cross_reference: http://www.sciencedirect.com/science/article/pii/016770129400068I

enzyme assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: enzyme assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: enzyme assay evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of enzyme assay evidence that is used in a manual assertion.
has_obo_namespace: eco
id: ECO:0005801

Axiom
annotated source: enzyme assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: enzyme assay evidence used in manual assertion
annotated property: definition
annotated target: A type of enzyme assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

cell transfection experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell transfection experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: cell transfection experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cell transfection experiment evidence that is used in a manual assertion.
has_obo_namespace: eco
id: ECO:0005802

Axiom
annotated source: cell transfection experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: cell transfection experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of cell transfection experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

motility assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where the motility of a cell or cells is determined.
has_obo_namespace: eco
id: ECO:0005803

Axiom
annotated source: motility assay evidence
annotated property: definition
annotated target: A type of direct assay evidence where the motility of a cell or cells is determined.
database_cross_reference: ECO:SN

immunofluorescence evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunofluorescence evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunofluorescence evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunofluorescence evidence that is used in a manual assertion.
has_obo_namespace: eco
id: ECO:0005804

Axiom
annotated source: immunofluorescence evidence used in manual assertion
annotated property: definition
annotated target: A type of immunofluorescence evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

yeast 2-hybrid evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: yeast 2-hybrid evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: yeast 2-hybrid evidence
subclass of: physical interaction evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of yeast 2-hybrid evidence that is used in a manual assertion.
has_obo_namespace: eco
id: ECO:0005805

Axiom
annotated source: yeast 2-hybrid evidence used in manual assertion
annotated property: definition
annotated target: A type of yeast 2-hybrid evidence that is used in a manual assertion.
database_cross_reference: ECO:SN

Cya fusion reporter assay evidence
type: Class
subclass of: ectopic expression evidence
subclass of: reporter gene assay evidence
definition: A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications.
created by: rctauber
creation date: 2016-06-15T08:42:16Z
has_obo_namespace: eco
id: ECO:0006001

Axiom
annotated source: Cya fusion reporter assay evidence
annotated property: definition
annotated target: A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications.
database_cross_reference: PMID:10217833
database_cross_reference: PMID:14702323

Cya fusion reporter assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Cya fusion reporter assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: ectopic expression evidence used in manual assertion
subclass of: Cya fusion reporter assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Cya fusion reporter assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2010-06-15T08:44:10Z
has_obo_namespace: eco
id: ECO:0006002

Axiom
annotated source: Cya fusion reporter assay evidence used in manual assertion
annotated property: subClassOf
annotated target: ectopic expression evidence used in manual assertion
is inferred: true

Axiom
annotated source: Cya fusion reporter assay evidence used in manual assertion
annotated property: definition
annotated target: A type of Cya fusion reporter assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

electron microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: electron microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: electron microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of electron microscopy evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-12T12:32:29Z
has_obo_namespace: eco
id: ECO:0006003

Axiom
annotated source: electron microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: electron microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of electron microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

super-resolution microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: super-resolution microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: super-resolution microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of super-resolution microscopy evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-12T12:32:29Z
has_obo_namespace: eco
id: ECO:0006004

Axiom
annotated source: super-resolution microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: super-resolution microscopy evidence used in manual assertion
annotated property: definition
annotated target: A type of super-resolution microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

fractionation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fractionation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: fractionation evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of fractionation evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-12T12:32:29Z
has_obo_namespace: eco
id: ECO:0006005

Axiom
annotated source: fractionation evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: fractionation evidence used in manual assertion
annotated property: definition
annotated target: A type of fractionation evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

electrophysiology assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: electrophysiology assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophysiology assay evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of electrophysiology assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-12T12:32:29Z
has_obo_namespace: eco
id: ECO:0006006

Axiom
annotated source: electrophysiology assay evidence used in manual assertion
annotated property: subClassOf
annotated target: direct assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: electrophysiology assay evidence used in manual assertion
annotated property: definition
annotated target: A type of electrophysiology assay evidence that is used in a manual assertion.
comment: ECO:RCT

chromatin immunoprecipitation-chip evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation-chip evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: chromatin immunoprecipitation-chip evidence
subclass of: immunoprecipitation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-21T11:55:53Z
has_obo_namespace: eco
id: ECO:0006007

Axiom
annotated source: chromatin immunoprecipitation-chip evidence used in manual assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

chromatin immunoprecipitation- exonuclease evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation- exonuclease evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: chromatin immunoprecipitation- exonuclease evidence
subclass of: immunoprecipitation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-21T11:55:53Z
has_obo_namespace: eco
id: ECO:0006008

Axiom
annotated source: chromatin immunoprecipitation- exonuclease evidence used in manual assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

chromatin immunoprecipitation-seq evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chromatin immunoprecipitation-seq evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: chromatin immunoprecipitation-seq evidence
subclass of: immunoprecipitation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-21T11:55:53Z
has_obo_namespace: eco
id: ECO:0006009

Axiom
annotated source: chromatin immunoprecipitation-seq evidence used in manual assertion
annotated property: definition
annotated target: A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

mRNA interactome capture evidence
type: Class
subclass of: nucleic acid binding evidence
definition: A type of nucleic acid binding evidence where RNA and proteins are cross-linked by ultraviolet light and RNA-protein complexes are captured on oligo(dT) beads. After RNAse treatment, the RNA-bound proteins are identified by mass spectrometry.
created by: rctauber
creation date: 2016-10-21T12:28:17Z
has_exact_synonym: ultraviolet light cross-linking RNA binding evidence
has_obo_namespace: eco
id: ECO:0006010

Axiom
annotated source: mRNA interactome capture evidence
annotated property: definition
annotated target: A type of nucleic acid binding evidence where RNA and proteins are cross-linked by ultraviolet light and RNA-protein complexes are captured on oligo(dT) beads. After RNAse treatment, the RNA-bound proteins are identified by mass spectrometry.
database_cross_reference: PMID:27729395

mRNA interactome capture evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mRNA interactome capture evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: mRNA interactome capture evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of mRNA interactome capture evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-10-21T12:28:17Z
has_obo_namespace: eco
id: ECO:0006011

Axiom
annotated source: mRNA interactome capture evidence used in manual assertion
annotated property: definition
annotated target: A type of mRNA interactome capture evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

patch-clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels.
created by: rctauber
creation date: 2016-11-28T11:44:05Z
has_obo_namespace: eco
id: ECO:0006012

Axiom
annotated source: patch-clamp recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels.
database_cross_reference: ECO:RCT

patch-clamp recording evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: patch-clamp recording evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophysiology assay evidence used in manual assertion
subclass of: patch-clamp recording evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of patch-clamp recording evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-11-28T11:44:05Z
has_obo_namespace: eco
id: ECO:0006013

Axiom
annotated source: patch-clamp recording evidence used in manual assertion
annotated property: definition
annotated target: A type of patch-clamp recording evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

whole-cell patch-clamp recording evidence
type: Class
subclass of: patch-clamp recording evidence
definition: A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell.
created by: rctauber
creation date: 2016-11-28T11:44:05Z
has_obo_namespace: eco
id: ECO:0006014

Axiom
annotated source: whole-cell patch-clamp recording evidence
annotated property: definition
annotated target: A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell.
database_cross_reference: PMID:27341060

whole-cell patch-clamp recording evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: whole-cell patch-clamp recording evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: patch-clamp recording evidence used in manual assertion
subclass of: whole-cell patch-clamp recording evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of whole-cell patch-clamp recording evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-11-28T11:44:05Z
has_obo_namespace: eco
id: ECO:0006015

Axiom
annotated source: whole-cell patch-clamp recording evidence used in manual assertion
annotated property: definition
annotated target: A type of whole-cell patch-clamp recording evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

traceable author statement from published clinical study
type: Class
subclass of: traceable author statement
definition: A type of traceable author statement that is based on a publication about a clinical study.
created by: rctauber
creation date: 2016-11-30T09:44:47Z
has_exact_synonym: published clinical study evidence
has_obo_namespace: eco
id: ECO:0006016

traceable author statement from published clinical study used in manual assertion
type: Class
equivalent class: Class
  intersection of: traceable author statement from published clinical study
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: traceable author statement used in manual assertion
subclass of: traceable author statement from published clinical study
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of traceable author statement from published clinical study that is used in a manual assertion.
created by: rctauber
creation date: 2016-11-30T09:44:47Z
has_exact_synonym: PCS
has_obo_namespace: eco
id: ECO:0006017
comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used when evidence for an HPO annotation is information extracted from articles in the medical literature.

Axiom
annotated source: traceable author statement from published clinical study used in manual assertion
annotated property: has_exact_synonym
annotated target: PCS
database_cross_reference: HPO

inference based on individual clinical experience
type: Class
subclass of: curator inference
definition: A type of curator inference that is based on the individual clinical experience of a clinician
created by: rctauber
creation date: 2016-11-30T09:44:47Z
has_exact_synonym: individual clinical experience evidence
has_obo_namespace: eco
id: ECO:0006018

inference based on individual clinical experience used in manual assertion
type: Class
equivalent class: Class
  intersection of: inference based on individual clinical experience
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: curator inference used in manual assertion
subclass of: inference based on individual clinical experience
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of inference based on individual clinical experience that is used in a manual assertion.
created by: rctauber
creation date: 2016-11-30T09:44:47Z
has_exact_synonym: ICE
has_obo_namespace: eco
id: ECO:0006019
comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used for annotating disorders with a limited amount of published data, and is accompanied by a reference to the individual or center performing the annotation.

Axiom
annotated source: inference based on individual clinical experience used in manual assertion
annotated property: has_exact_synonym
annotated target: ICE
database_cross_reference: HPO

biofilm formation assay evidence
type: Class
subclass of: in vitro culture assay evidence
definition: A type of in vitro culture assay evidence in which biofilm growth is monitored and detected from attachment to development.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006020

Axiom
annotated source: biofilm formation assay evidence
annotated property: definition
annotated target: A type of in vitro culture assay evidence in which biofilm growth is monitored and detected from attachment to development.
database_cross_reference: PMID:10547784

biofilm formation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biofilm formation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: biofilm formation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of biofilm formation assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006021

Axiom
annotated source: biofilm formation assay evidence used in manual assertion
annotated property: definition
annotated target: A type of biofilm formation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

microtiter plate biofilm assay evidence
type: Class
subclass of: biofilm formation assay evidence
definition: A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006022

Axiom
annotated source: microtiter plate biofilm assay evidence
annotated property: definition
annotated target: A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes.
database_cross_reference: PMID:10547784

microtiter plate biofilm assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: microtiter plate biofilm assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biofilm formation assay evidence used in manual assertion
subclass of: microtiter plate biofilm assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of microtiter plate biofilm assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006023

Axiom
annotated source: microtiter plate biofilm assay evidence used in manual assertion
annotated property: definition
annotated target: A type of microtiter plate biofilm assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

air-liquid interface assay evidence
type: Class
subclass of: biofilm formation assay evidence
definition: A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_exact_synonym: ALI biofilm assay evidence
has_obo_namespace: eco
id: ECO:0006024
comment: Tilting of the plate positions the air-liquid interface on a clear portion to improve visability.

Axiom
annotated source: air-liquid interface assay evidence
annotated property: definition
annotated target: A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate.
database_cross_reference: PMID:18770545

air-liquid interface assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: air-liquid interface assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biofilm formation assay evidence used in manual assertion
subclass of: air-liquid interface assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of air-liquid interface assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006025

Axiom
annotated source: air-liquid interface assay evidence used in manual assertion
annotated property: definition
annotated target: A type of air-liquid interface assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

colony biofilm assay evidence
type: Class
subclass of: biofilm formation assay evidence
definition: A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate to continously supply nutrients by relocating to fresh plates.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006026
comment: The plates commonly contain different carbon sources or antibiotic treatments to observe properties of the cells.

Axiom
annotated source: colony biofilm assay evidence
annotated property: definition
annotated target: A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate to continously supply nutrients by relocating to fresh plates.
database_cross_reference: PMID:18770545

colony biofilm assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: colony biofilm assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biofilm formation assay evidence used in manual assertion
subclass of: colony biofilm assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of colony biofilm assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006027

Axiom
annotated source: colony biofilm assay evidence used in manual assertion
annotated property: definition
annotated target: A type of colony biofilm assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

Kadouri drip-fed biofilm assay evidence
type: Class
subclass of: biofilm formation assay evidence
definition: A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006028

Axiom
annotated source: Kadouri drip-fed biofilm assay evidence
annotated property: definition
annotated target: A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out.
database_cross_reference: PMID:18770545

Kadouri drip-fed biofilm assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Kadouri drip-fed biofilm assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biofilm formation assay evidence used in manual assertion
subclass of: Kadouri drip-fed biofilm assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-12-05T09:33:18Z
has_obo_namespace: eco
id: ECO:0006029

Axiom
annotated source: Kadouri drip-fed biofilm assay evidence used in manual assertion
annotated property: definition
annotated target: A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

co-immunoprecipitation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-immunoprecipitation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: co-immunoprecipitation evidence
subclass of: physical interaction evidence used in manual assertion
subclass of: immunoprecipitation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of co-immunoprecipitation evidence that is used in a manual assertion.
created by: rctauber
creation date: 2016-01-13T11:32:26Z
has_obo_namespace: eco
id: ECO:0006030

Axiom
annotated source: co-immunoprecipitation evidence used in manual assertion
annotated property: subClassOf
annotated target: physical interaction evidence used in manual assertion
is inferred: true

Axiom
annotated source: co-immunoprecipitation evidence used in manual assertion
annotated property: subClassOf
annotated target: immunoprecipitation evidence used in manual assertion
is inferred: true

Axiom
annotated source: co-immunoprecipitation evidence used in manual assertion
annotated property: definition
annotated target: A type of co-immunoprecipitation evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

immunolocalization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunolocalization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunolocalization evidence
subclass of: microscopy evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of immunolocalization evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T07:54:37Z
has_obo_namespace: eco
id: ECO:0006031

Axiom
annotated source: immunolocalization evidence used in manual assertion
annotated property: subClassOf
annotated target: microscopy evidence used in manual assertion
is inferred: true

Axiom
annotated source: immunolocalization evidence used in manual assertion
annotated property: definition
annotated target: A type of immunolocalization evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

optogenetic evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_exact_synonym: optogenetic actuator evidence
has_obo_namespace: eco
id: ECO:0006032

Axiom
annotated source: optogenetic evidence
annotated property: definition
annotated target: A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators.
database_cross_reference: GO:DOS
database_cross_reference: PMID:17035522

optogenetic evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: optogenetic evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: optogenetic evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of optogenetic evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006033

Axiom
annotated source: optogenetic evidence used in manual assertion
annotated property: subClassOf
annotated target: experimental evidence used in manual assertion
is inferred: true

Axiom
annotated source: optogenetic evidence used in manual assertion
annotated property: definition
annotated target: A type of optogenetic evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

fluorescent sensor evidence
type: Class
subclass of: fluorescence evidence
definition: A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006034
comment: Examples include sensors for ion concentration and potential difference across a membrane.

Axiom
annotated source: fluorescent sensor evidence
annotated property: definition
annotated target: A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor.
database_cross_reference: GO:DOS

fluorescent sensor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescent sensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: fluorescence evidence used in manual assertion
subclass of: fluorescent sensor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of fluorescent sensor evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006035

Axiom
annotated source: fluorescent sensor evidence used in manual assertion
annotated property: subClassOf
annotated target: fluorescence evidence used in manual assertion
is inferred: true

Axiom
annotated source: fluorescent sensor evidence used in manual assertion
annotated property: definition
annotated target: A type of fluorescent sensor evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

genetically encoded fluorescent sensor evidence
type: Class
subclass of: fluorescent sensor evidence
definition: A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_exact_synonym: optogenetic sensor evidence
has_obo_namespace: eco
id: ECO:0006036

Axiom
annotated source: genetically encoded fluorescent sensor evidence
annotated property: definition
annotated target: A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor.
database_cross_reference: GO:DOS

genetically encoded fluorescent sensor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetically encoded fluorescent sensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: fluorescent sensor evidence used in manual assertion
subclass of: genetically encoded fluorescent sensor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006037

Axiom
annotated source: genetically encoded fluorescent sensor evidence used in manual assertion
annotated property: subClassOf
annotated target: fluorescent sensor evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetically encoded fluorescent sensor evidence used in manual assertion
annotated property: definition
annotated target: A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

genetically encoded fluorescent electrophysiology assay evidence
type: Class
subclass of: electrophysiology assay evidence
subclass of: fluorescent sensor evidence
definition: A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_exact_synonym: electrophysiology - optical assay evidence
has_obo_namespace: eco
id: ECO:0006038
comment: Examples include genetically encoded sensors that detect potential difference across plasma membrane.

Axiom
annotated source: genetically encoded fluorescent electrophysiology assay evidence
annotated property: definition
annotated target: A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors.
database_cross_reference: GO:DOS

genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetically encoded fluorescent electrophysiology assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophysiology assay evidence used in manual assertion
subclass of: fluorescent sensor evidence used in manual assertion
subclass of: genetically encoded fluorescent electrophysiology assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006039

Axiom
annotated source: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
annotated property: subClassOf
annotated target: electrophysiology assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
annotated property: subClassOf
annotated target: fluorescent sensor evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
annotated property: definition
annotated target: A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

genetically encoded fluorescent ion concentration sensor assay evidence
type: Class
subclass of: genetically encoded fluorescent electrophysiology assay evidence
definition: A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006040
comment: Examples include the genetically encoded calcium indicator GCaMP.

Axiom
annotated source: genetically encoded fluorescent ion concentration sensor assay evidence
annotated property: definition
annotated target: A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor.
database_cross_reference: GO:DOS

genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetically encoded fluorescent ion concentration sensor assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
subclass of: genetically encoded fluorescent ion concentration sensor assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-01-23T11:58:48Z
has_obo_namespace: eco
id: ECO:0006041

Axiom
annotated source: genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion
annotated property: subClassOf
annotated target: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion
annotated property: definition
annotated target: A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

cell fractionation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell fractionation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: cell fractionation evidence
subclass of: fractionation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of cell fractionation evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006042

Axiom
annotated source: cell fractionation evidence used in manual assertion
annotated property: subClassOf
annotated target: fractionation evidence used in manual assertion
is inferred: true

Axiom
annotated source: cell fractionation evidence used in manual assertion
annotated property: definition
annotated target: A type of cell fractionation evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

extracellular recording evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006043

Axiom
annotated source: extracellular recording evidence
annotated property: definition
annotated target: A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons.
database_cross_reference: url:http://www.nature.com/subjects/extracellular-recording

extracellular recording evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: extracellular recording evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophysiology assay evidence used in manual assertion
subclass of: extracellular recording evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of extracellular recording evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006044

Axiom
annotated source: extracellular recording evidence used in manual assertion
annotated property: subClassOf
annotated target: electrophysiology assay evidence used in manual assertion
is inferred: true

Axiom
annotated source: extracellular recording evidence used in manual assertion
annotated property: definition
annotated target: A type of extracellular recording evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

single-unit extracellular recording evidence
type: Class
subclass of: extracellular recording evidence
definition: A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006045

Axiom
annotated source: single-unit extracellular recording evidence
annotated property: definition
annotated target: A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron.
database_cross_reference: doi:10.1385/0-89603-185-3:1

single-unit extracellular recording evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: single-unit extracellular recording evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: extracellular recording evidence used in manual assertion
subclass of: single-unit extracellular recording evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of single-unit extracellular recording evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006046

Axiom
annotated source: single-unit extracellular recording evidence used in manual assertion
annotated property: subClassOf
annotated target: extracellular recording evidence used in manual assertion
is inferred: true

Axiom
annotated source: single-unit extracellular recording evidence used in manual assertion
annotated property: definition
annotated target: A type of single-unit extracellular recording evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

field potential recording evidence
type: Class
subclass of: extracellular recording evidence
definition: A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_narrow_synonym: local field potential recording evidence
has_obo_namespace: eco
id: ECO:0006047

Axiom
annotated source: field potential recording evidence
annotated property: definition
annotated target: A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes.
database_cross_reference: ECO:RCT

field potential recording evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: field potential recording evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: extracellular recording evidence used in manual assertion
subclass of: field potential recording evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of field potential recording evidence that is used in a manual assertion.
created by: rctauber
creation date: 2017-02-21T07:26:28Z
has_obo_namespace: eco
id: ECO:0006048

Axiom
annotated source: field potential recording evidence used in manual assertion
annotated property: subClassOf
annotated target: extracellular recording evidence used in manual assertion
is inferred: true

Axiom
annotated source: field potential recording evidence used in manual assertion
annotated property: definition
annotated target: A type of field potential recording evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

genetic transformation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetic transformation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: mutant phenotype evidence used in manual assertion
subclass of: genetic transformation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of genetic transformation evidence that is used in a manual assertion
created by: rctauber
creation date: 02-21-2017T18:98:58Z
has_obo_namespace: eco
id: ECO:0006049

Axiom
annotated source: genetic transformation evidence used in manual assertion
annotated property: subClassOf
annotated target: mutant phenotype evidence used in manual assertion
is inferred: true

Axiom
annotated source: genetic transformation evidence used in manual assertion
annotated property: definition
annotated target: A type of genetic transformation evidence that is used in a manual assertion
database_cross_reference: ECO:RCT

anti-sense experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: anti-sense experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: anti-sense experiment evidence
subclass of: mutant phenotype evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of anti-sense experiment evidence that is used in a manual assertion.
created by: rctauber
creation date: 02-21-2017T18:98:58Z
has_obo_namespace: eco
id: ECO:0006050

Axiom
annotated source: anti-sense experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: mutant phenotype evidence used in manual assertion
is inferred: true

Axiom
annotated source: anti-sense experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of anti-sense experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

morpholino experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: morpholino experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: morpholino experiment evidence
subclass of: mutant phenotype evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of morpholino experiment evidence that is used in a manual assertion.
created by: rctauber
creation date: 02-21-2017T18:98:58Z
has_obo_namespace: eco
id: ECO:0006051

Axiom
annotated source: morpholino experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: mutant phenotype evidence used in manual assertion
is inferred: true

Axiom
annotated source: morpholino experiment evidence used in manual assertion
annotated property: definition
annotated target: A type of morpholino experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

RNAi evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNAi evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: RNAi evidence
subclass of: mutant phenotype evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of RNAi evidence that is used in a manual assertion.
created by: rctauber
creation date: 02-21-2017T18:98:58Z
has_obo_namespace: eco
id: ECO:0006052

Axiom
annotated source: RNAi evidence used in manual assertion
annotated property: subClassOf
annotated target: mutant phenotype evidence used in manual assertion
is inferred: true

Axiom
annotated source: RNAi evidence used in manual assertion
annotated property: definition
annotated target: A type of RNAi evidence that is used in a manual assertion.
database_cross_reference: ECO:RCT

high throughput evidence
type: Class
subclass of: evidence
definition: A type of evidence where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
created by: mchibucos
creation date: 2017-03-28T14:48:39Z
has_exact_synonym: HT evidence
has_exact_synonym: high-throughput evidence
has_narrow_synonym: high throughput cell biology evidence
has_narrow_synonym: high throughput screening evidence
has_obo_namespace: eco
id: ECO:0006055
comment: Some relevant articles include: PMID: 23340846, doi:10.1038/nmeth0607-523

Axiom
annotated source: high throughput evidence
annotated property: definition
annotated target: A type of evidence where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
database_cross_reference: ECO:MCC

high throughput evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: high throughput evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: high throughput evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
created by: rctauber
creation date: 2017-03-28T14:48:39Z
has_obo_namespace: eco
id: ECO:0006056

Axiom
annotated source: high throughput evidence used in manual assertion
annotated property: subClassOf
annotated target: evidence used in manual assertion
is inferred: true

Axiom
annotated source: high throughput evidence used in manual assertion
annotated property: definition
annotated target: A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
database_cross_reference: ECO:RCT

high throughput evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: high throughput evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence used in automatic assertion
subclass of: high throughput evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
created by: rctauber
creation date: 2017-03-28T14:48:39Z
has_obo_namespace: eco
id: ECO:0006057

Axiom
annotated source: high throughput evidence used in automatic assertion
annotated property: subClassOf
annotated target: evidence used in automatic assertion
is inferred: true

Axiom
annotated source: high throughput evidence used in automatic assertion
annotated property: definition
annotated target: A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel.
database_cross_reference: ECO:RCT

high throughput cell biology evidence
type: Class
subclass of: high throughput evidence
definition: A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
created by: mchibucos
creation date: 2017-03-28T14:48:39Z
has_obo_namespace: eco
has_related_synonym: omics experiment
id: ECO:0006058
comment: HT methodologies may incorporate techniques from optics, chemistry, biology or image analysis (https://en.wikipedia.org/wiki/High_throughput_biology)

Axiom
annotated source: high throughput cell biology evidence
annotated property: definition
annotated target: A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
database_cross_reference: ECO:MCC

high throughput cell biology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: high throughput cell biology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: high throughput evidence used in manual assertion
subclass of: high throughput cell biology evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
created by: rctauber
creation date: 2017-03-28T14:48:39Z
has_obo_namespace: eco
id: ECO:0006059

Axiom
annotated source: high throughput cell biology evidence used in manual assertion
annotated property: subClassOf
annotated target: high throughput evidence used in manual assertion
is inferred: true

Axiom
annotated source: high throughput cell biology evidence used in manual assertion
annotated property: definition
annotated target: A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
database_cross_reference: ECO:RCT

high throughput cell biology evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: high throughput cell biology evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: high throughput evidence used in automatic assertion
subclass of: high throughput cell biology evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
created by: rctauber
creation date: 2017-03-28T14:48:39Z
has_obo_namespace: eco
id: ECO:0006060

Axiom
annotated source: high throughput cell biology evidence used in automatic assertion
annotated property: subClassOf
annotated target: high throughput evidence used in automatic assertion
is inferred: true

Axiom
annotated source: high throughput cell biology evidence used in automatic assertion
annotated property: definition
annotated target: A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying large numbers of biomolecules.
database_cross_reference: ECO:RCT