eco.owl
type: Ontology
has_obo_format_version: 1.2
date: 18:03:2016 13:15
comment: For more details, see https://github.com/evidenceontology/evidenceontology
auto-generated-by: OBO-Edit 2.3.1
default-namespace: eco
saved-by: snadendla
version IRI: eco.owl

definition
type: AnnotationProperty

valid with biological process
type: AnnotationProperty
comment: GO biological process terms should be used in the GO with/from field
sub property of: subset_property

valid with cellular component
type: AnnotationProperty
comment: GO cellular component terms should be used in the GO with/from field
sub property of: subset_property

valid with chemical entity
type: AnnotationProperty
comment: Chemical entity IDs should be used in the GO with/from field
sub property of: subset_property

valid with gene
type: AnnotationProperty
comment: Gene IDs should be used in the GO with/from field
sub property of: subset_property

valid with molecular function
type: AnnotationProperty
comment: GO molecular function terms should be used in the GO with/from field
sub property of: subset_property

valid with protein
type: AnnotationProperty
comment: Protein IDs should be used in the GO with/from field
sub property of: subset_property

valid with protein complex
type: AnnotationProperty
comment: Protein complex IDs should be used in the GO with/from field
sub property of: subset_property

valid with transcript
type: AnnotationProperty
comment: Transcript IDs should be used in the GO with/from field
sub property of: subset_property

subset_property
type: AnnotationProperty

has_alternative_id
type: AnnotationProperty

has_broad_synonym
type: AnnotationProperty

database_cross_reference
type: AnnotationProperty

has_exact_synonym
type: AnnotationProperty

has_narrow_synonym
type: AnnotationProperty

has_obo_format_version
type: AnnotationProperty

has_obo_namespace
type: AnnotationProperty

has_related_synonym
type: AnnotationProperty

in_subset
type: AnnotationProperty

shorthand
type: AnnotationProperty

used_in
type: ObjectProperty
creation date: 2010-12-09T05:00:20Z
definition: A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence.
database_cross_reference: ECO:9000000
comment: In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI.
has_obo_namespace: eco
created by: mchibucos
id: used_in
shorthand: used_in
domain: evidence
range: assertion method

Axiom
annotated target: A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence.
database_cross_reference: GOC:cjm
annotated source: used_in
annotated property: definition

uses
type: ObjectProperty
database_cross_reference: ECO:9000001
has_obo_namespace: eco
id: uses
shorthand: uses
inverse of: used_in

evidence
type: Class
disjoint with: assertion method
definition: A type of information that is used to support an assertion.
id: ECO:0000000
has_obo_namespace: eco
has_related_synonym: evidence code
has_related_synonym: evidence_code

Axiom
annotated target: A type of information that is used to support an assertion.
database_cross_reference: ECO:MCC
annotated source: evidence
annotated property: definition

inference from background scientific knowledge
type: Class
subclass of: curator inference
definition: A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator.
id: ECO:0000001
has_exact_synonym: IC
has_obo_namespace: eco
has_exact_synonym: inferred by curator

Axiom
database_cross_reference: GO:IC
annotated target: inferred by curator
annotated source: inference from background scientific knowledge
annotated property: has_exact_synonym

Axiom
annotated target: A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go
annotated source: inference from background scientific knowledge
annotated property: definition

Axiom
database_cross_reference: GO:IC
annotated target: IC
annotated source: inference from background scientific knowledge
annotated property: has_exact_synonym

direct assay evidence
type: Class
subclass of: experimental evidence
id: ECO:0000002
definition: Experimental evidence that is based on direct measurement of some aspect of a biological feature.
has_obo_namespace: eco

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence that is based on direct measurement of some aspect of a biological feature.
database_cross_reference: GO:IDA
annotated source: direct assay evidence
annotated property: definition

reconstitution assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state.
id: ECO:0000003
has_obo_namespace: eco

Axiom
annotated target: A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state.
database_cross_reference: ECO:KAV
database_cross_reference: PMID:26029343
annotated source: reconstitution assay evidence
annotated property: definition

cell fractionation evidence
type: Class
subclass of: fractionation evidence
definition: A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient.
id: ECO:0000004
has_related_synonym: IDA: cell fractionation
comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology.
has_obo_namespace: eco

Axiom
annotated target: A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient.
database_cross_reference: ECO:KIM
database_cross_reference: TAIR:TED
annotated source: cell fractionation evidence
annotated property: definition

enzyme assay evidence
type: Class
subclass of: protein assay evidence
id: ECO:0000005
has_related_synonym: IDA: enzyme assays
database_cross_reference: PSI-MI:MI:0415
definition: Used when an annotation is made based on assays that determine the catalytic activity of enzymes.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on assays that determine the catalytic activity of enzymes.
annotated source: enzyme assay evidence
annotated property: definition

enzymatic study
type: Axiom
annotated target: PSI-MI:MI:0415
annotated source: enzyme assay evidence
annotated property: database_cross_reference

experimental evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on the results of a laboratory assay.
id: ECO:0000006
has_exact_synonym: EXP
has_obo_namespace: eco
has_exact_synonym: inferred from experiment

Axiom
annotated target: EXP
database_cross_reference: GO:EXP
annotated source: experimental evidence
annotated property: has_exact_synonym

Axiom
annotated target: An evidence type that is based on the results of a laboratory assay.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:ecd
annotated source: experimental evidence
annotated property: definition

Axiom
database_cross_reference: GO:EXP
annotated target: inferred from experiment
annotated source: experimental evidence
annotated property: has_exact_synonym

immunofluorescence evidence
type: Class
subclass of: protein detection assay evidence
definition: A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell.
id: ECO:0000007
has_related_synonym: IDA: immunofluorescence
has_obo_namespace: eco

Axiom
annotated target: A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: immunofluorescence evidence
annotated property: definition

expression pattern evidence
type: Class
subclass of: experimental evidence
id: ECO:0000008
definition: Experimental evidence that is based on characterization of gene expression.
has_exact_synonym: IEP
comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process.
has_obo_namespace: eco
has_exact_synonym: inferred from expression pattern

Axiom
database_cross_reference: GO:IEP
annotated target: IEP
annotated source: expression pattern evidence
annotated property: has_exact_synonym

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence that is based on characterization of gene expression.
database_cross_reference: GO:IEP
annotated source: expression pattern evidence
annotated property: definition

Axiom
database_cross_reference: GO:IEP
annotated target: inferred from expression pattern
annotated source: expression pattern evidence
annotated property: has_exact_synonym

transcript expression evidence
type: Class
subclass of: expression pattern evidence
id: ECO:0000009
has_obo_namespace: eco

protein expression evidence
type: Class
subclass of: expression pattern evidence
subclass of: protein assay evidence
id: ECO:0000010
has_obo_namespace: eco

genetic interaction evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000011
database_cross_reference: TAIR:TED:0000006
has_obo_namespace: eco

functional complementation evidence
type: Class
subclass of: genetic interaction evidence
id: ECO:0000012
has_related_synonym: IGI: functional complementation
definition: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene.
annotated source: functional complementation evidence
annotated property: definition

transgenic rescue experiment evidence
type: Class
subclass of: functional complementation evidence
id: ECO:0000013
has_obo_namespace: eco

mutant phenotype evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000015
definition: The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible.
has_obo_namespace: eco

Axiom
database_cross_reference: GO:IMP
annotated target: The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible.
annotated source: mutant phenotype evidence
annotated property: definition

loss-of-function mutant phenotype evidence
type: Class
subclass of: mutant phenotype evidence
id: ECO:0000016
has_obo_namespace: eco

ectopic expression evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000017
has_related_synonym: IMP: analysis of overexpression/ectopic expression phenotype
definition: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question.
annotated source: ectopic expression evidence
annotated property: definition

anti-sense experiment evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000018
has_related_synonym: IMP: anti-sense experiments
definition: Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background.
annotated source: anti-sense experiment evidence
annotated property: definition

RNAi evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000019
has_related_synonym: IMP: RNAi experiment
definition: Inference based on a phenotype observed when expressing an RNAi construct that includes a fragment of the gene product in a wild-type (for that gene product) background. A double-stranded RNA specifically suppresses the expression of the gene bearing its complementary sequence.
has_obo_namespace: eco

Axiom
annotated target: Inference based on a phenotype observed when expressing an RNAi construct that includes a fragment of the gene product in a wild-type (for that gene product) background. A double-stranded RNA specifically suppresses the expression of the gene bearing its complementary sequence.
database_cross_reference: TAIR:TED
annotated source: RNAi evidence
annotated property: definition

specific protein inhibition evidence
type: Class
subclass of: protein assay evidence
definition: A type of protein assay evidence based on the inhibition of the molecular function of a protein.
id: ECO:0000020
has_obo_namespace: eco

Axiom
annotated target: A type of protein assay evidence based on the inhibition of the molecular function of a protein.
database_cross_reference: ECO:MCC
annotated source: specific protein inhibition evidence
annotated property: definition

physical interaction evidence
type: Class
subclass of: experimental evidence
id: ECO:0000021
definition: Experimental evidence that is based on characterization of an interaction between a gene product and another molecule.
comment: Molecules interacted with might include protein, nucleic acid, ion, or complex.
database_cross_reference: PSI-MI:MI:0045
has_obo_namespace: eco

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence that is based on characterization of an interaction between a gene product and another molecule.
annotated source: physical interaction evidence
annotated property: definition

experimental interaction detection
type: Axiom
annotated target: PSI-MI:MI:0045
annotated source: physical interaction evidence
annotated property: database_cross_reference

co-purification evidence
type: Class
subclass of: physical interaction evidence
definition: A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex.
id: ECO:0000022
has_related_synonym: IPI: co-purification
database_cross_reference: PSI-MI:MI:0025
has_obo_namespace: eco

copurification
type: Axiom
annotated target: PSI-MI:MI:0025
annotated source: co-purification evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex.
database_cross_reference: TAIR:TED
annotated source: co-purification evidence
annotated property: definition

affinity evidence
type: Class
subclass of: physical interaction evidence
definition: A type of physical interaction evidence that depends on the strength of the interaction between two entities.
id: ECO:0000023
database_cross_reference: PSI-MI:MI:0400
has_obo_namespace: eco
has_narrow_synonym: ligand binding evidence

affinity technology
type: Axiom
annotated target: PSI-MI:MI:0400
annotated source: affinity evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of physical interaction evidence that depends on the strength of the interaction between two entities.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0400
annotated source: affinity evidence
annotated property: definition

protein binding evidence
type: Class
subclass of: affinity evidence
subclass of: protein assay evidence
id: ECO:0000024
has_obo_namespace: eco

hybrid interaction evidence
type: Class
subclass of: affinity evidence
definition: A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.
id: ECO:0000025
database_cross_reference: PSI-MI:MI:0090
comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode.
has_obo_namespace: eco

protein complementation assay
type: Axiom
annotated target: PSI-MI:MI:0090
annotated source: hybrid interaction evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0090
annotated source: hybrid interaction evidence
annotated property: definition

nucleic acid hybridization evidence
type: Class
subclass of: experimental genomic evidence
id: ECO:0000026
has_obo_namespace: eco

structural similarity evidence
type: Class
subclass of: phenotypic similarity evidence
definition: A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes.
id: ECO:0000027
comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field.
has_obo_namespace: eco

Axiom
annotated target: A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: structural similarity evidence
annotated property: definition

motif similarity evidence
type: Class
subclass of: match to sequence model evidence
id: ECO:0000028
has_related_synonym: ISS: recognized domains
definition: Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence.
annotated source: motif similarity evidence
annotated property: definition

match to InterPro member signature evidence
type: Class
subclass of: match to sequence model evidence
id: ECO:0000029
has_obo_namespace: eco

BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence
subclass of: pairwise sequence alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of BLAST evidence that is used in a manual assertion.
id: ECO:0000030
has_related_synonym: curated BLAST analysis
has_obo_namespace: eco

Axiom
is inferred: true
annotated source: BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of BLAST evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: BLAST evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: BLAST evidence used in manual assertion
annotated target: pairwise sequence alignment evidence used in manual assertion
annotated property: subClassOf

protein BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence used in manual assertion
subclass of: protein BLAST evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
id: ECO:0000031
database_cross_reference: GO_REF:0000012
database_cross_reference: GO_REF:0000027
definition: Protein BLAST evidence that is used in a manual assertion.
has_related_synonym: curated protein BLAST analysis
has_obo_namespace: eco

Axiom
database_cross_reference: ECO:MCC
annotated target: Protein BLAST evidence that is used in a manual assertion.
annotated source: protein BLAST evidence used in manual assertion
annotated property: definition

Pairwise alignment (TIGR)
type: Axiom
annotated target: GO_REF:0000012
annotated source: protein BLAST evidence used in manual assertion
annotated property: database_cross_reference

BLAST search criteria for ISS assignment in PAMGO_GAT
type: Axiom
annotated target: GO_REF:0000027
annotated source: protein BLAST evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated target: BLAST evidence used in manual assertion
annotated source: protein BLAST evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: protein BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

nucleotide BLAST evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nucleotide BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: BLAST evidence used in manual assertion
subclass of: nucleotide BLAST evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
id: ECO:0000032
definition: Nucleotide BLAST evidence that is used in a manual assertion.
has_related_synonym: curated nucleic acid BLAST analysis
has_obo_namespace: eco

Axiom
is inferred: true
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: BLAST evidence used in manual assertion
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Nucleotide BLAST evidence that is used in a manual assertion.
annotated source: nucleotide BLAST evidence used in manual assertion
annotated property: definition

traceable author statement
type: Class
subclass of: author statement
disjoint with: non-traceable author statement
definition: An author statement that is based on a cited reference.
id: ECO:0000033
comment: The traceable author statement (TAS) evidence code covers author statements that are attributed to a cited source. Typically this type of information comes from review articles. Material from the introductions and discussion sections of non-review papers may also be suitable if another reference is cited as the source of experimental work or analysis. When annotating with this code the curator should use caution and be aware that authors often cite papers dealing with experiments that were performed in organisms different from the one being discussed in the paper at hand. Thus a problem with the TAS code is that it may turn out from following up the references in the paper that no experiments were performed on the gene in the organism actually being characterized in the primary paper. For this reason we recommend (when time and resources allow) that curators track down the cited paper and annotate directly from the experimental paper using the appropriate experimental evidence code. When this is not possible and it is necessary to annotate from reviews, the TAS code is the appropriate code to use for statements that are associated with a cited reference. Once an annotation has been made to a given term using an experimental evidence code, we recommend removing any annotations made to the same term using the TAS evidence code.
has_obo_namespace: eco

Axiom
annotated target: An author statement that is based on a cited reference.
database_cross_reference: ECO:MCC
database_cross_reference: GO:TAS
annotated source: traceable author statement
annotated property: definition

non-traceable author statement
type: Class
subclass of: author statement
definition: An author statement that is not associated with results presented or a cited reference.
id: ECO:0000034
comment: The non-traceable author statement evidence code should be used in all cases where the author makes a statement that a curator wants to capture but for which there are neither results presented nor a specific reference cited in the source used to make the annotation. The source of the information may be peer reviewed papers, textbooks, database records or vouchered specimens.
has_obo_namespace: eco

Axiom
annotated target: An author statement that is not associated with results presented or a cited reference.
database_cross_reference: ECO:MCC
annotated source: non-traceable author statement
annotated property: definition

no biological data found
type: Class
subclass of: curator inference
definition: A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources.
comment: An assertion of "no biological data found" carries the assumption that a more-or-less exhaustive search has been conducted.
id: ECO:0000035
has_exact_synonym: ND
has_obo_namespace: eco
has_exact_synonym: no biological data available

Axiom
database_cross_reference: GO:ND
annotated target: no biological data available
annotated source: no biological data found
annotated property: has_exact_synonym

Axiom
database_cross_reference: GO:ND
annotated target: ND
annotated source: no biological data found
annotated property: has_exact_synonym

Axiom
annotated target: A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources.
database_cross_reference: ECO:MCC
database_cross_reference: GO:ND
annotated source: no biological data found
annotated property: definition

not_recorded
type: Class
definition: An evidence that reflects an annotation was made before curators began tracking evidence types.
id: ECO:0000037
has_exact_synonym: NR
comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations.
has_obo_namespace: eco
has_exact_synonym: not recorded
deprecated: true

Axiom
database_cross_reference: GO:NR
annotated target: NR
annotated source: not_recorded
annotated property: has_exact_synonym

Axiom
database_cross_reference: GO:NR
annotated target: not recorded
annotated source: not_recorded
annotated property: has_exact_synonym

Axiom
annotated target: An evidence that reflects an annotation was made before curators began tracking evidence types.
database_cross_reference: ECO:MCC
database_cross_reference: GO:NR
annotated source: not_recorded
annotated property: definition

transient rescue experiment evidence
type: Class
subclass of: functional complementation evidence
id: ECO:0000038
has_obo_namespace: eco

protein assay evidence
type: Class
subclass of: direct assay evidence
id: ECO:0000039
has_obo_namespace: eco

immunological assay evidence
type: Class
subclass of: affinity evidence
id: ECO:0000040
has_obo_namespace: eco

similarity evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on comparing likeness of distinct biological entities.
id: ECO:0000041
has_exact_synonym: IS
has_obo_namespace: eco
has_related_synonym: inferred from similarity

Axiom
annotated target: An evidence type that is based on comparing likeness of distinct biological entities.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IS
annotated source: similarity evidence
annotated property: definition

gain-of-function mutant phenotype evidence
type: Class
subclass of: mutant phenotype evidence
id: ECO:0000042
has_obo_namespace: eco

sequence similarity evidence
type: Class
subclass of: similarity evidence
comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes.
definition: A type of similarity based on biomolecular sequence.
id: ECO:0000044
has_broad_synonym: ISS
has_obo_namespace: eco
has_broad_synonym: inferred from sequence or structural similarity
has_exact_synonym: inferred from sequence similarity

Axiom
database_cross_reference: GO:ISS
annotated target: ISS
annotated source: sequence similarity evidence
annotated property: has_broad_synonym

Axiom
database_cross_reference: GO:ISS
annotated target: inferred from sequence or structural similarity
annotated source: sequence similarity evidence
annotated property: has_broad_synonym

Axiom
annotated target: A type of similarity based on biomolecular sequence.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: sequence similarity evidence
annotated property: definition

spatial pattern of protein expression evidence
type: Class
subclass of: protein expression evidence
id: ECO:0000045
has_obo_namespace: eco

protein expression level evidence
type: Class
subclass of: protein expression evidence
id: ECO:0000046
has_obo_namespace: eco

spatial pattern of transcript expression evidence
type: Class
subclass of: transcript expression evidence
id: ECO:0000047
has_obo_namespace: eco

transcript expression level evidence
type: Class
subclass of: transcript expression evidence
id: ECO:0000048
has_obo_namespace: eco

reporter gene assay evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000049
has_related_synonym: IEP: expression of a reporter gene
definition: Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response.
annotated source: reporter gene assay evidence
annotated property: definition

voucher specimen analysis evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000050
has_exact_synonym: IVS
definition: Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:DOA
annotated target: Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype.
annotated source: voucher specimen analysis evidence
annotated property: definition

genetic similarity evidence
type: Class
subclass of: similarity evidence
comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology.
definition: A type of similarity based on genotype without respect to expression.
id: ECO:0000051
has_exact_synonym: IGTS
has_obo_namespace: eco
has_related_synonym: inferred from genetic similarity

Axiom
annotated target: A type of similarity based on genotype without respect to expression.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IGTS
annotated source: genetic similarity evidence
annotated property: definition

suppressor/enhancer interaction evidence
type: Class
subclass of: genetic interaction evidence
id: ECO:0000052
has_related_synonym: IGI: 'traditional' genetic interactions (e.g. suppressors, synthetic lethals)
database_cross_reference: TAIR:TED:0000063
has_obo_namespace: eco

computational combinatorial evidence
type: Class
subclass of: combinatorial evidence
definition: A type of combinatorial analysis where data are combined and evaluated by an algorithm.
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.
id: ECO:0000053
database_cross_reference: TAIR:TED:0000004
has_obo_namespace: eco

Axiom
annotated target: A type of combinatorial analysis where data are combined and evaluated by an algorithm.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go
annotated source: computational combinatorial evidence
annotated property: definition

double mutant phenotype evidence
type: Class
subclass of: genetic interaction evidence
id: ECO:0000054
has_related_synonym: IGI: double mutant analysis
definition: Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIT:TED
annotated target: Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus.
annotated source: double mutant phenotype evidence
annotated property: definition

array experiment evidence
type: Class
subclass of: expression pattern evidence
id: ECO:0000055
has_obo_namespace: eco

epistatic interaction evidence
type: Class
subclass of: genetic interaction evidence
id: ECO:0000056
has_related_synonym: IGI: epistatic interactions
definition: Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus.
annotated source: epistatic interaction evidence
annotated property: definition

phenotypic similarity evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity based on the expression of a genotype in an environment.
id: ECO:0000057
has_exact_synonym: IPTS
comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms.
has_obo_namespace: eco
has_related_synonym: inferred from phenotypic similarity

Axiom
annotated target: A type of similarity based on the expression of a genotype in an environment.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IPTS
annotated source: phenotypic similarity evidence
annotated property: definition

expression microarray evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000058
has_obo_namespace: eco

experimental phenotypic evidence
type: Class
subclass of: experimental evidence
has_alternative_id: ECO:0000014
id: ECO:0000059
definition: Experimental evidence that is based on the expression of a genotype in an environment.
has_obo_namespace: eco
has_related_synonym: inferred from phenotype

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence that is based on the expression of a genotype in an environment.
annotated source: experimental phenotypic evidence
annotated property: definition

positional similarity evidence
type: Class
subclass of: phenotypic similarity evidence
id: ECO:0000060
has_exact_synonym: IPS
definition: Used when an annotation is made based on the similarity of the location and or arrangement of structures.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:IPS
annotated target: Used when an annotation is made based on the similarity of the location and or arrangement of structures.
annotated source: positional similarity evidence
annotated property: definition

quantitative trait analysis evidence
type: Class
subclass of: experimental phenotypic evidence
definition: A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus.
id: ECO:0000061
has_related_synonym: IGI: quantitative trait analysis
has_obo_namespace: eco

Axiom
annotated target: A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus.
database_cross_reference: TAIR:tn
annotated source: quantitative trait analysis evidence
annotated property: definition

genomic microarray evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000062
has_obo_namespace: eco

compositional similarity evidence
type: Class
subclass of: phenotypic similarity evidence
id: ECO:0000063
has_exact_synonym: ICS
definition: Used when an annotation is made based on the similarity of the histological makeup of structures.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:ICS
annotated target: Used when an annotation is made based on the similarity of the histological makeup of structures.
annotated source: compositional similarity evidence
annotated property: definition

functional complementation in heterologous system evidence
type: Class
subclass of: functional complementation evidence
id: ECO:0000064
has_related_synonym: IGI: functional complementation in heterologous system
definition: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene.
annotated source: functional complementation in heterologous system evidence
annotated property: definition

CpG island microarray evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000065
has_obo_namespace: eco

yeast one-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
definition: A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors.
id: ECO:0000066
has_related_synonym: IPI: yeast one-hybrid assay
database_cross_reference: PSI-MI:MI:0432
comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED).
has_obo_namespace: eco

one hybrid
type: Axiom
annotated target: PSI-MI:MI:0432
annotated source: yeast one-hybrid evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors.
database_cross_reference: ECO:MCC
database_cross_reference: PSI-MI:MI:0432
database_cross_reference: TAIR:TED
annotated source: yeast one-hybrid evidence
annotated property: definition

developmental similarity evidence
type: Class
subclass of: phenotypic similarity evidence
id: ECO:0000067
has_exact_synonym: IDS
definition: Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:IDS
annotated target: Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures.
annotated source: developmental similarity evidence
annotated property: definition

yeast 2-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
id: ECO:0000068
has_related_synonym: IPI: yeast two-hybrid assay
definition: Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed.
annotated source: yeast 2-hybrid evidence
annotated property: definition

differential methylation hybridization evidence
type: Class
subclass of: nucleic acid hybridization evidence
id: ECO:0000069
has_obo_namespace: eco

co-immunoprecipitation evidence
type: Class
subclass of: immunoprecipitation evidence
definition: A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification.
id: ECO:0000070
has_related_synonym: IPI: co-immunoprecipitation
comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column.
database_cross_reference: PSI-MI:MI:0019
has_obo_namespace: eco

Axiom
annotated target: A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification.
database_cross_reference: ECO:MCC
annotated source: co-immunoprecipitation evidence
annotated property: definition

coimmunoprecipitation
type: Axiom
annotated target: PSI-MI:MI:0019
annotated source: co-immunoprecipitation evidence
annotated property: database_cross_reference

morphological similarity evidence
type: Class
subclass of: phenotypic similarity evidence
id: ECO:0000071
has_exact_synonym: IMS
definition: Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:IMS
annotated target: Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures.
annotated source: morphological similarity evidence
annotated property: definition

Sos-recruitment assay evidence
type: Class
definition: A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout.
id: ECO:0000072
has_related_synonym: IPI: Sos-recruitment assay
has_obo_namespace: eco
deprecated: true

Axiom
annotated target: A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout.
database_cross_reference: TAIR:TED
annotated source: Sos-recruitment assay evidence
annotated property: definition

experimental genomic evidence
type: Class
subclass of: experimental evidence
id: ECO:0000073
definition: Experimental evidence that characterizes an attribute of the genome underlying a gene product.
has_obo_namespace: eco
has_exact_synonym: inferred from genomic analysis

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence that characterizes an attribute of the genome underlying a gene product.
annotated source: experimental genomic evidence
annotated property: definition

split-ubiquitin assay evidence
type: Class
subclass of: physical interaction evidence
id: ECO:0000074
has_related_synonym: IPI: split-ubiquitin assay
database_cross_reference: PSI-MI:MI:0112
definition: Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs.
has_obo_namespace: eco

ubiquitin reconstruction
type: Axiom
annotated target: PSI-MI:MI:0112
annotated source: split-ubiquitin assay evidence
annotated property: database_cross_reference

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs.
annotated source: split-ubiquitin assay evidence
annotated property: definition

gene expression similarity evidence
type: Class
subclass of: phenotypic similarity evidence
id: ECO:0000075
has_exact_synonym: IGES
definition: Used when an annotation is made based on the similarity of expression of genes in structures.
has_obo_namespace: eco

Axiom
database_cross_reference: PhenoScape:IGES
annotated target: Used when an annotation is made based on the similarity of expression of genes in structures.
annotated source: gene expression similarity evidence
annotated property: definition

far-Western blotting evidence
type: Class
subclass of: physical interaction evidence
id: ECO:0000076
has_related_synonym: IPI: far-Western analysis
database_cross_reference: PSI-MI:MI:0047
definition: Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column.
annotated source: far-Western blotting evidence
annotated property: definition

methylation-specific polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
id: ECO:0000077
has_obo_namespace: eco
has_exact_synonym: methylation-specific PCR evidence

southern hybridization evidence
type: Class
subclass of: DNA detection assay evidence
id: ECO:0000078
has_related_synonym: ISS: Southern blotting
has_exact_synonym: Southern blot
definition: Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question.
annotated source: southern hybridization evidence
annotated property: definition

affinity chromatography evidence
type: Class
subclass of: affinity evidence
comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand.
definition: A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound.
id: ECO:0000079
has_related_synonym: IDA: affinity chromatography
database_cross_reference: PSI-MI:MI:0004
has_obo_namespace: eco

Axiom
annotated target: A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: affinity chromatography evidence
annotated property: definition

affinity chromatography technology
type: Axiom
annotated target: PSI-MI:MI:0004
annotated source: affinity chromatography evidence
annotated property: database_cross_reference

phylogenetic evidence
type: Class
subclass of: similarity evidence
definition: A type of similarity that indicates common ancestry.
id: ECO:0000080
has_exact_synonym: IP
has_obo_namespace: eco

Axiom
annotated target: A type of similarity that indicates common ancestry.
database_cross_reference: ECO:MCC
database_cross_reference: PhenoScape:IP
annotated source: phylogenetic evidence
annotated property: definition

Axiom
annotated target: IP
database_cross_reference: PhenoScape:IP
annotated source: phylogenetic evidence
annotated property: has_exact_synonym

targeting sequence prediction evidence
type: Class
subclass of: motif similarity evidence
id: ECO:0000081
has_related_synonym: ISS: targeting sequence prediction
definition: Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence.
annotated source: targeting sequence prediction evidence
annotated property: definition

polymerase chain reaction evidence
type: Class
subclass of: experimental genomic evidence
id: ECO:0000082
has_exact_synonym: PCR evidence
has_obo_namespace: eco

transmembrane domain prediction evidence
type: Class
subclass of: motif similarity evidence
id: ECO:0000083
has_related_synonym: ISS: transmembrane domain prediction
definition: Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence.
annotated source: transmembrane domain prediction evidence
annotated property: definition

gene neighbors evidence
type: Class
subclass of: genomic context evidence
creation date: 2009-03-20T11:55:18Z
id: ECO:0000084
definition: Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes.
database_cross_reference: GO_REF:0000025
comment: Genomic cluster analyses include synteny and operon structure.
has_related_synonym: ICL
has_obo_namespace: eco
has_exact_synonym: inferred from genome cluster
created by: mgiglio

Axiom
database_cross_reference: ECO:MCC
annotated target: Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes.
database_cross_reference: GOC:mg
annotated source: gene neighbors evidence
annotated property: definition

Operon structure as IGC evidence
type: Axiom
annotated target: GO_REF:0000025
annotated source: gene neighbors evidence
annotated property: database_cross_reference

immunoprecipitation evidence
type: Class
subclass of: protein assay evidence
definition: A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation.
id: ECO:0000085
has_related_synonym: IDA: immunoprecipitation
comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding.
has_obo_namespace: eco

Axiom
annotated target: A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: TAIR:TED
annotated source: immunoprecipitation evidence
annotated property: definition

intermethylated site amplification evidence
type: Class
subclass of: experimental genomic evidence
id: ECO:0000086
has_obo_namespace: eco

immunolocalization evidence
type: Class
subclass of: microscopy evidence
subclass of: immunodetection assay evidence
definition: A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues.
id: ECO:0000087
has_related_synonym: IDA: immunolocalization
has_obo_namespace: eco

Axiom
annotated target: A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SN
annotated source: immunolocalization evidence
annotated property: definition

biological system reconstruction evidence
type: Class
subclass of: evidence
creation date: 2009-03-20T12:00:17Z
definition: A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system.
id: ECO:0000088
has_related_synonym: ISR
comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak.
has_obo_namespace: eco
has_related_synonym: inferred from system reconstruction
created by: mgiglio

Axiom
annotated target: A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:mg
annotated source: biological system reconstruction evidence
annotated property: definition

restriction landmark genomic scanning evidence
type: Class
subclass of: DNA detection assay evidence
id: ECO:0000089
has_alternative_id: ECO:0001125
has_exact_synonym: RLGS evidence
has_obo_namespace: eco
has_exact_synonym: restriction landmark genome scanning evidence

immunogold labelling evidence
type: Class
subclass of: immunolocalization evidence
definition: A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy.
id: ECO:0000090
has_related_synonym: IDA: immunogold labelling
has_obo_namespace: eco

Axiom
annotated target: A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy.
database_cross_reference: ECO:MCC
annotated source: immunogold labelling evidence
annotated property: definition

epitope-tagged protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
id: ECO:0000092
has_related_synonym: IDA: immunolocalization of epitope-tagged protein
definition: Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies.
annotated source: epitope-tagged protein immunolocalization evidence
annotated property: definition

oligonucleotide microarray evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000093
has_obo_namespace: eco

biological assay evidence
type: Class
subclass of: direct assay evidence
definition: Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition.
id: ECO:0000094
has_related_synonym: IDA: bioassay
has_obo_namespace: eco

Axiom
annotated target: Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: biological assay evidence
annotated property: definition

cell growth assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-10-15T00:58:50Z
definition: A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect.
comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter.
id: ECO:0000095
has_obo_namespace: eco
has_related_synonym: growth curve analysis
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect.
database_cross_reference: ECO:SW
database_cross_reference: PomBase:MAH
annotated source: cell growth assay evidence
annotated property: definition

electrophoretic mobility shift assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions.
id: ECO:0000096
comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid.
has_exact_synonym: EMSA: electrophoretic mobility shift assay
has_related_synonym: Gel retardation assay
has_related_synonym: IDA: electrophoretic mobility shift assay
database_cross_reference: PSI-MI:MI:0413
has_obo_namespace: eco

Axiom
annotated target: Gel retardation assay
database_cross_reference: PMID:17703195
annotated source: electrophoretic mobility shift assay evidence
annotated property: has_related_synonym

Axiom
annotated target: A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions.
database_cross_reference: ECO:KIM
database_cross_reference: ECO:SW
database_cross_reference: PMID:17703195
database_cross_reference: TAIR:TED
annotated source: electrophoretic mobility shift assay evidence
annotated property: definition

electrophoretic mobility shift assay
type: Axiom
annotated target: PSI-MI:MI:0413
annotated source: electrophoretic mobility shift assay evidence
annotated property: database_cross_reference

cDNA microarray evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000097
has_obo_namespace: eco

in situ hybridization evidence
type: Class
subclass of: nucleic acid hybridization evidence
id: ECO:0000098
has_related_synonym: IDA: in situ hybridization
definition: Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence.
annotated source: in situ hybridization evidence
annotated property: definition

fractionation evidence
type: Class
subclass of: direct assay evidence
id: ECO:0000100
has_obo_namespace: eco

Affymetrix array experiment evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000101
has_obo_namespace: eco

co-fractionation evidence
type: Class
subclass of: fractionation evidence
id: ECO:0000102
has_related_synonym: IDA: co-fractionation
definition: Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules.
annotated source: co-fractionation evidence
annotated property: definition

microarray RNA expression level evidence
type: Class
subclass of: transcript expression level evidence
subclass of: expression microarray evidence
id: ECO:0000104
has_related_synonym: IEP: transcript levels (e.g. microarray data)
definition: Used when an annotation is made based on levels of RNA expression determined by microarray experiment.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on levels of RNA expression determined by microarray experiment.
annotated source: microarray RNA expression level evidence
annotated property: definition

Nimblegen array evidence
type: Class
subclass of: array experiment evidence
id: ECO:0000105
has_obo_namespace: eco

northern assay evidence
type: Class
subclass of: transcript expression level evidence
definition: A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples.
id: ECO:0000106
has_related_synonym: IEP: transcript levels (e.g. Northerns)
has_exact_synonym: Northern blot
has_obo_namespace: eco

Axiom
annotated target: A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples.
database_cross_reference: ECO:SW
database_cross_reference: TAIR:TED
annotated source: northern assay evidence
annotated property: definition

reverse transcription polymerase chain reaction transcription evidence
type: Class
subclass of: transcript expression level evidence
subclass of: reverse transcription polymerase chain reaction evidence
id: ECO:0000108
has_related_synonym: IEP: transcript levels (e.g. RT-PCR)
has_exact_synonym: RT-PCR transcription evidence
definition: Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise).
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise).
annotated source: reverse transcription polymerase chain reaction transcription evidence
annotated property: definition

reverse transcription polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
definition: A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR.
id: ECO:0000109
has_exact_synonym: RT-PCR evidence
comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate.
has_obo_namespace: eco
has_exact_synonym: qRT-PCR

Axiom
database_cross_reference: PMID:12901609
annotated target: qRT-PCR
annotated source: reverse transcription polymerase chain reaction evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11013345
database_cross_reference: PMID:12901609
annotated source: reverse transcription polymerase chain reaction evidence
annotated property: definition

RNA protection assay evidence
type: Class
subclass of: nuclease protection assay evidence
definition: A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography.
id: ECO:0000110
has_related_synonym: IEP: RNA protection assay
has_related_synonym: RNAse protection assay
has_narrow_synonym: RPA
has_obo_namespace: eco
has_exact_synonym: ribonuclease protection assay

Axiom
annotated target: A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23457339
database_cross_reference: TAIR:TED
annotated source: RNA protection assay evidence
annotated property: definition

western blot evidence
type: Class
subclass of: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
subclass of: immunodetection assay evidence
definition: A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique.
id: ECO:0000112
has_related_synonym: IEP: protein levels (e.g. Western blots)
has_related_synonym: Western blot expression analysis
comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies.
has_obo_namespace: eco
has_related_synonym: protein immunoblot

Axiom
annotated target: A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23050259
database_cross_reference: TAIR:TED
annotated source: western blot evidence
annotated property: definition

expression library screen evidence
type: Class
subclass of: protein expression evidence
definition: A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein.
id: ECO:0000114
has_related_synonym: IEP: expression library screening
has_obo_namespace: eco

Axiom
annotated target: A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein.
database_cross_reference: ECO:MCC
database_cross_reference: TAIR:TED
annotated source: expression library screen evidence
annotated property: definition

differential hybridization evidence
type: Class
subclass of: transcript expression evidence
subclass of: nucleic acid hybridization evidence
id: ECO:0000116
has_related_synonym: IEP: differential hybridization
definition: Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another.
annotated source: differential hybridization evidence
annotated property: definition

subtractive hybridization evidence
type: Class
subclass of: transcript expression evidence
subclass of: nucleic acid hybridization evidence
id: ECO:0000118
has_related_synonym: IEP: subtractive hybridization
definition: Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another.
annotated source: subtractive hybridization evidence
annotated property: definition

over expression analysis evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000120
has_related_synonym: IMP: analysis of overexpression/ectopic expression phenotype
definition: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question.
annotated source: over expression analysis evidence
annotated property: definition

protein localization evidence
type: Class
subclass of: protein assay evidence
id: ECO:0000122
has_obo_namespace: eco

fusion protein localization evidence
type: Class
subclass of: protein localization evidence
id: ECO:0000124
has_obo_namespace: eco

green fluorescent protein fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
id: ECO:0000126
has_exact_synonym: GFP fusion protein localization evidence
has_related_synonym: IDA: localization of GFP/YFP fusion protein
has_obo_namespace: eco

yellow fluorescent protein fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
id: ECO:0000128
has_related_synonym: IDA: localization of GFP/YFP fusion protein
has_exact_synonym: YFP fusion protein localization evidence
has_obo_namespace: eco

beta-glucuronidase fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
id: ECO:0000130
has_exact_synonym: GUS fusion protein localization evidence
has_narrow_synonym: GUS staining evidence
has_related_synonym: IDA: localization of GUS fusion protein
has_obo_namespace: eco

beta-galactosidase fusion protein localization evidence
type: Class
subclass of: fusion protein localization evidence
id: ECO:0000132
has_exact_synonym: LacZ fusion protein localization evidence
has_obo_namespace: eco

transport assay evidence
type: Class
subclass of: direct assay evidence
id: ECO:0000134
has_related_synonym: IDA: transport assay
has_obo_namespace: eco

nucleic acid binding evidence
type: Class
subclass of: affinity evidence
id: ECO:0000136
has_obo_namespace: eco

ribohomopolymer binding assay evidence
type: Class
subclass of: nucleic acid binding evidence
id: ECO:0000138
has_related_synonym: IDA: ribohomopolymer binding assay
has_obo_namespace: eco

thin layer chromatography evidence
type: Class
subclass of: chromatography evidence
definition: A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate.
id: ECO:0000140
has_related_synonym: IDA: thin layer chromatography
has_exact_synonym: TLC evidence
has_obo_namespace: eco

Axiom
annotated target: A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate.
database_cross_reference: ECO:MCC
annotated source: thin layer chromatography evidence
annotated property: definition

protein:ion binding evidence
type: Class
subclass of: protein binding evidence
id: ECO:0000142
has_obo_namespace: eco

Southwestern blot evidence
type: Class
subclass of: nucleic acid binding evidence
id: ECO:0000144
has_related_synonym: IDA: Southwestern analysis
definition: Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins.
annotated source: Southwestern blot evidence
annotated property: definition

Northwestern blot evidence
type: Class
subclass of: nucleic acid binding evidence
id: ECO:0000146
has_related_synonym: IDA: Northwestern analysis
definition: Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins.
annotated source: Northwestern blot evidence
annotated property: definition

in vitro binding evidence
type: Class
id: ECO:0000148
has_related_synonym: IDA: in vitro binding assay
has_obo_namespace: eco
deprecated: true

in vitro transcription reconstitution assay evidence
type: Class
subclass of: reconstitution assay evidence
id: ECO:0000150
has_related_synonym: IDA: in vitro reconstitution assay with recombinant protein
has_obo_namespace: eco

in vitro recombinant protein transcription reconstitution assay evidence
type: Class
subclass of: in vitro transcription reconstitution assay evidence
id: ECO:0000152
has_related_synonym: IDA: in vitro reconstitution assay with recombinant protein
has_obo_namespace: eco

heterologous protein expression evidence
type: Class
subclass of: protein expression evidence
definition: A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed.
id: ECO:0000154
has_related_synonym: IDA: protein expression in heterologous system
has_obo_namespace: eco

Axiom
annotated target: A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed.
database_cross_reference: ECO:KAV
annotated source: heterologous protein expression evidence
annotated property: definition

protein separation evidence
type: Class
subclass of: fractionation evidence
id: ECO:0000156
has_obo_namespace: eco

protein separation followed by direct sequencing evidence
type: Class
subclass of: protein separation evidence
id: ECO:0000158
has_related_synonym: IDA: protein separation and direct sequencing
has_obo_namespace: eco

protein separation followed by fragment identification evidence
type: Class
subclass of: protein separation evidence
id: ECO:0000160
has_related_synonym: IDA: protein separation and fragment identification
has_obo_namespace: eco

heterologous system uptake evidence
type: Class
subclass of: transport assay evidence
id: ECO:0000162
has_related_synonym: IDA: uptake assay in heterologous system
definition: Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein.
annotated source: heterologous system uptake evidence
annotated property: definition

electrophysiology assay evidence
type: Class
subclass of: direct assay evidence
definition: A type of direct assay evidence where electrical properties of cells or tissues are studied.
id: ECO:0000164
comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart.
has_obo_namespace: eco

Axiom
annotated target: A type of direct assay evidence where electrical properties of cells or tissues are studied.
database_cross_reference: ECO:KAV
annotated source: electrophysiology assay evidence
annotated property: definition

two-electrode voltage clamp experiment evidence
type: Class
subclass of: electrophysiology assay evidence
definition: A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current.
id: ECO:0000166
has_related_synonym: IDA: two-electrode voltage clamp technique
has_obo_namespace: eco

Axiom
annotated target: A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current.
database_cross_reference: ECO:SN
database_cross_reference: PMID:23529422
annotated source: two-electrode voltage clamp experiment evidence
annotated property: definition

transcription assay
type: Class
subclass of: direct assay evidence
id: ECO:0000168
has_obo_namespace: eco

transcriptional activation assay
type: Class
subclass of: transcription assay
id: ECO:0000170
has_related_synonym: IDA: transcriptional activation assay
definition: Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene.
annotated source: transcriptional activation assay
annotated property: definition

biochemical trait analysis evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000172
has_related_synonym: IMP: analysis of biochemical trait
definition: Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate.
annotated source: biochemical trait analysis evidence
annotated property: definition

mutant physiological response evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000174
has_related_synonym: IMP: analysis of physiological response
definition: Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions.
annotated source: mutant physiological response evidence
annotated property: definition

mutant visible phenotype evidence
type: Class
subclass of: experimental phenotypic evidence
id: ECO:0000176
has_related_synonym: IMP: analysis of visible trait
definition: Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology.
has_obo_namespace: eco

Axiom
database_cross_reference: TAIR:TED
annotated target: Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology.
annotated source: mutant visible phenotype evidence
annotated property: definition

genomic context evidence
type: Class
subclass of: evidence
definition: An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome.
id: ECO:0000177
comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis.
has_obo_namespace: eco

Axiom
annotated target: An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:go
annotated source: genomic context evidence
annotated property: definition

in vivo assay evidence
type: Class
subclass of: biological assay evidence
id: ECO:0000178
has_obo_namespace: eco

animal model system study evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence arising from the investigation of an animal model system.
id: ECO:0000179
has_obo_namespace: eco

Axiom
annotated target: A type of experimental evidence arising from the investigation of an animal model system.
database_cross_reference: ECO:MCC
annotated source: animal model system study evidence
annotated property: definition

clinical study evidence
type: Class
subclass of: experimental evidence
definition: A type of experimental evidence arising from a controlled investigation that uses human subjects.
id: ECO:0000180
has_obo_namespace: eco

Axiom
annotated target: A type of experimental evidence arising from a controlled investigation that uses human subjects.
database_cross_reference: ECO:MCC
annotated source: clinical study evidence
annotated property: definition

in vitro assay evidence
type: Class
subclass of: biological assay evidence
id: ECO:0000181
has_obo_namespace: eco

in vitro culture assay evidence
type: Class
subclass of: biological assay evidence
id: ECO:0000182
has_obo_namespace: eco

cell-free assay evidence
type: Class
subclass of: in vitro assay evidence
id: ECO:0000183
has_obo_namespace: eco
has_broad_synonym: in vitro assay

enzyme inhibition evidence
type: Class
subclass of: enzyme assay evidence
subclass of: specific protein inhibition evidence
definition: A type of specific protein inhibition evidence where the protein under study is an enzyme.
id: ECO:0000184
has_obo_namespace: eco

Axiom
annotated target: A type of specific protein inhibition evidence where the protein under study is an enzyme.
database_cross_reference: ECO:MCC
annotated source: enzyme inhibition evidence
annotated property: definition

sequence alignment evidence
type: Class
subclass of: sequence similarity evidence
creation date: 2010-03-18T12:21:31Z
definition: A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments.
has_alternative_id: ECO:00000057
id: ECO:0000200
has_exact_synonym: ISA
has_obo_namespace: eco
has_exact_synonym: inferred from sequence alignment
created by: mchibucos

Axiom
database_cross_reference: GO:ISA
annotated target: ISA
annotated source: sequence alignment evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments.
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml
annotated source: sequence alignment evidence
annotated property: definition

Axiom
database_cross_reference: GO:ISA
annotated target: inferred from sequence alignment
annotated source: sequence alignment evidence
annotated property: has_exact_synonym

sequence orthology evidence
type: Class
subclass of: sequence similarity evidence
creation date: 2010-03-18T12:30:06Z
has_alternative_id: ECO:00000060
id: ECO:0000201
has_exact_synonym: ISO
definition: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors.
has_obo_namespace: eco
has_exact_synonym: inferred from sequence orthology
created by: mchibucos
has_related_synonym: ortholog evidence

Axiom
database_cross_reference: GO:ISO
annotated target: inferred from sequence orthology
annotated source: sequence orthology evidence
annotated property: has_exact_synonym

Axiom
annotated target: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors.
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml
annotated source: sequence orthology evidence
annotated property: definition

Axiom
database_cross_reference: GO:ISO
annotated target: ISO
annotated source: sequence orthology evidence
annotated property: has_exact_synonym

match to sequence model evidence
type: Class
subclass of: sequence similarity evidence
creation date: 2010-03-18T12:32:30Z
has_alternative_id: ECO:00000063
id: ECO:0000202
has_exact_synonym: ISM
definition: Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product.
comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN.
has_obo_namespace: eco
has_exact_synonym: inferred from sequence model
created by: mchibucos

Axiom
database_cross_reference: GO:ISM
annotated target: ISM
annotated source: match to sequence model evidence
annotated property: has_exact_synonym

Axiom
database_cross_reference: GO:ISM
annotated target: inferred from sequence model
annotated source: match to sequence model evidence
annotated property: has_exact_synonym

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product.
database_cross_reference: url:http://www.geneontology.org/GO.evidence.shtml
annotated source: match to sequence model evidence
annotated property: definition

automatic assertion
type: Class
subclass of: assertion method
disjoint with: manual assertion
creation date: 2010-03-18T12:36:04Z
definition: An assertion method that does not involve human review.
comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically.
has_alternative_id: ECO:00000067
id: ECO:0000203
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: An assertion method that does not involve human review.
database_cross_reference: ECO:MCC
annotated source: automatic assertion
annotated property: definition

author statement
type: Class
equivalent class: Class
  union of: traceable author statement
  union of: non-traceable author statement
subclass of: evidence
creation date: 2010-06-21T11:17:21Z
definition: An evidence type that is based on an assertion by the author of a paper, which is read by a curator.
id: ECO:0000204
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: An evidence type that is based on an assertion by the author of a paper, which is read by a curator.
database_cross_reference: ECO:MCC
annotated source: author statement
annotated property: definition

curator inference
type: Class
subclass of: inferential evidence
creation date: 2010-08-18T05:55:12Z
definition: A type of inferential evidence that is based on a conclusion drawn by a curator.
id: ECO:0000205
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of inferential evidence that is based on a conclusion drawn by a curator.
database_cross_reference: ECO:MCC
annotated source: curator inference
annotated property: definition

BLAST evidence
type: Class
subclass of: pairwise sequence alignment evidence
creation date: 2010-08-06T04:48:24Z
definition: A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST).
id: ECO:0000206
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST).
database_cross_reference: ECO:MCC
annotated source: BLAST evidence
annotated property: definition

nucleotide BLAST evidence
type: Class
subclass of: BLAST evidence
creation date: 2010-08-06T04:49:58Z
definition: BLAST evidence from aligning nucleotide sequence with nucleotide sequence.
id: ECO:0000207
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: BLAST evidence from aligning nucleotide sequence with nucleotide sequence.
database_cross_reference: ECO:MCC
annotated source: nucleotide BLAST evidence
annotated property: definition

protein BLAST evidence
type: Class
subclass of: BLAST evidence
creation date: 2010-08-06T04:50:45Z
definition: BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence.
id: ECO:0000208
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence.
database_cross_reference: ECO:MCC
annotated source: protein BLAST evidence
annotated property: definition

BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: BLAST evidence
subclass of: pairwise sequence alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-08-06T04:55:21Z
definition: A type of BLAST evidence that is used in an automatic assertion.
id: ECO:0000209
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated source: BLAST evidence used in automatic assertion
annotated target: pairwise sequence alignment evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of BLAST evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: BLAST evidence used in automatic assertion
annotated property: definition

nucleotide BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: nucleotide BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: nucleotide BLAST evidence
subclass of: BLAST evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-08-06T05:09:51Z
id: ECO:0000210
definition: Nucleotide BLAST evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: BLAST evidence used in automatic assertion
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
database_cross_reference: ECO:MCC
annotated target: Nucleotide BLAST evidence that is used in an automatic assertion.
annotated source: nucleotide BLAST evidence used in automatic assertion
annotated property: definition

protein BLAST evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: protein BLAST evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: protein BLAST evidence
subclass of: BLAST evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-08-06T05:11:04Z
id: ECO:0000211
definition: Protein BLAST evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Protein BLAST evidence that is used in an automatic assertion.
annotated source: protein BLAST evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated target: BLAST evidence used in automatic assertion
annotated source: protein BLAST evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: protein BLAST evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

combinatorial evidence
type: Class
subclass of: evidence
creation date: 2010-08-06T05:24:57Z
comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence.
definition: A type of evidence that arises out of the integration of more than one line of evidence.
has_alternative_id: ECO:0000036
has_alternative_id: ECO:0000043
id: ECO:0000212
has_obo_namespace: eco
has_related_synonym: inferred from in-silico analysis
created by: mchibucos

Axiom
annotated target: A type of evidence that arises out of the integration of more than one line of evidence.
database_cross_reference: ECO:MCC
annotated source: combinatorial evidence
annotated property: definition

combinatorial evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: combinatorial evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-08-06T05:28:33Z
definition: A type of combinatorial analysis that is used in an automatic assertion.
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.
id: ECO:0000213
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
annotated target: A type of combinatorial analysis that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: combinatorial evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: combinatorial evidence used in automatic assertion
annotated target: evidence used in automatic assertion
annotated property: subClassOf

biological aspect of descendant evidence
type: Class
subclass of: phylogenetic evidence
creation date: 2010-10-05T11:16:50Z
definition: A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene.
id: ECO:0000214
comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene.
database_cross_reference: ECO:MCC
annotated source: biological aspect of descendant evidence
annotated property: definition

rapid divergence from ancestral sequence evidence
type: Class
subclass of: phylogenetic evidence
creation date: 2010-10-05T03:41:34Z
definition: A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event.
id: ECO:0000215
comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event.
database_cross_reference: ECO:MCC
annotated source: rapid divergence from ancestral sequence evidence
annotated property: definition

phylogenetic determination of loss of key residues evidence
type: Class
subclass of: phylogenetic evidence
creation date: 2010-10-05T03:43:17Z
definition: A type of phylogenetic evidence characterized by the absence of key sequence residues.
id: ECO:0000216
comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phylogenetic evidence characterized by the absence of key sequence residues.
database_cross_reference: ECO:MCC
annotated source: phylogenetic determination of loss of key residues evidence
annotated property: definition

assertion method
type: Class
equivalent class: Class
  union of: automatic assertion
  union of: manual assertion
creation date: 2010-11-12T04:17:37Z
definition: A means by which a statement is made about an entity.
id: ECO:0000217
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A means by which a statement is made about an entity.
database_cross_reference: ECO:MCC
annotated source: assertion method
annotated property: definition

manual assertion
type: Class
subclass of: assertion method
creation date: 2010-11-12T04:20:02Z
comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information.
definition: An assertion method that involves human review.
id: ECO:0000218
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: An assertion method that involves human review.
database_cross_reference: ECO:MCC
annotated source: manual assertion
annotated property: definition

nucleotide sequencing assay evidence
type: Class
subclass of: sequencing assay evidence
creation date: 2010-11-15T12:22:40Z
definition: A sequencing assay result for a biopolymer that comprises nucleotides.
has_related_synonym: DNA sequencing
id: ECO:0000219
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A sequencing assay result for a biopolymer that comprises nucleotides.
database_cross_reference: ECO:MCC
annotated source: nucleotide sequencing assay evidence
annotated property: definition

Axiom
annotated target: DNA sequencing
database_cross_reference: OBI:0000626
annotated source: nucleotide sequencing assay evidence
annotated property: has_related_synonym

sequencing assay evidence
type: Class
subclass of: experimental evidence
creation date: 2010-11-15T01:44:45Z
definition: A type of experimental evidence where the order of molecules that constitute a biopolymer is determined.
id: ECO:0000220
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of experimental evidence where the order of molecules that constitute a biopolymer is determined.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0600047
annotated source: sequencing assay evidence
annotated property: definition

high throughput nucleotide sequencing assay evidence
type: Class
subclass of: nucleotide sequencing assay evidence
creation date: 2010-11-15T02:19:34Z
definition: A nucleotide sequencing assay result that is derived from a parallelized sequencing technique.
id: ECO:0000221
comment: Typically thousands to millions of reads are produced in parallel.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A nucleotide sequencing assay result that is derived from a parallelized sequencing technique.
database_cross_reference: ECO:MCC
annotated source: high throughput nucleotide sequencing assay evidence
annotated property: definition

Illumina sequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
creation date: 2010-11-15T02:38:03Z
definition: A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.
id: ECO:0000222
comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging.
has_related_synonym: Solexa sequencing result
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.
database_cross_reference: ECO:MCC
annotated source: Illumina sequencing evidence
annotated property: definition

Axiom
database_cross_reference: OBI:0000724
annotated target: Solexa sequencing result
annotated source: Illumina sequencing evidence
annotated property: has_related_synonym

454 pyrosequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
creation date: 2010-11-15T03:50:14Z
comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.
definition: A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology.
id: ECO:0000223
has_obo_namespace: eco
created by: mchibucos
has_broad_synonym: pyrosequencing

Axiom
annotated target: A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology.
database_cross_reference: ECO:MCC
annotated source: 454 pyrosequencing evidence
annotated property: definition

Axiom
database_cross_reference: OBI:0000730
annotated target: pyrosequencing
annotated source: 454 pyrosequencing evidence
annotated property: has_broad_synonym

SOLiD sequencing evidence
type: Class
subclass of: high throughput nucleotide sequencing assay evidence
creation date: 2010-11-15T04:17:41Z
definition: A high throughput nucleotide sequencing result derived by SOLiD sequencing technology.
id: ECO:0000224
comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A high throughput nucleotide sequencing result derived by SOLiD sequencing technology.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0000706
annotated source: SOLiD sequencing evidence
annotated property: definition

chain termination sequencing evidence
type: Class
subclass of: nucleotide sequencing assay evidence
creation date: 2010-11-15T04:46:31Z
definition: A nucleotide sequencing assay result derived by the chain termination sequencing method.
comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.
id: ECO:0000225
has_exact_synonym: Sanger sequencing
has_exact_synonym: dye terminator sequencing
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A nucleotide sequencing assay result derived by the chain termination sequencing method.
database_cross_reference: ECO:MCC
database_cross_reference: OBI:0000695
annotated source: chain termination sequencing evidence
annotated property: definition

chromatin immunoprecipitation evidence
type: Class
subclass of: immunoprecipitation evidence
creation date: 2010-11-16T01:29:08Z
definition: A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence.
has_exact_synonym: ChIP evidence
comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq).
id: ECO:0000226
database_cross_reference: PSI-MI:MI:0402
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence.
database_cross_reference: ECO:MCC
annotated source: chromatin immunoprecipitation evidence
annotated property: definition

chromatin immunoprecipitation assay
type: Axiom
annotated target: PSI-MI:MI:0402
annotated source: chromatin immunoprecipitation evidence
annotated property: database_cross_reference

chromatin immunoprecipitation-PCR evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
creation date: 2010-11-16T05:02:00Z
definition: A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection.
has_exact_synonym: ChIP-PCR evidence
comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results.
id: ECO:0000227
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:18388953
annotated source: chromatin immunoprecipitation-PCR evidence
annotated property: definition

chromatin immunoprecipitation-qPCR evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
creation date: 2010-11-16T05:26:47Z
definition: A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection.
has_exact_synonym: ChIP-qPCR evidence
id: ECO:0000228
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection.
database_cross_reference: ECO:MCC
annotated source: chromatin immunoprecipitation-qPCR evidence
annotated property: definition

chromatin immunoprecipitation-seq evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
creation date: 2010-11-16T05:30:41Z
definition: A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins.
has_exact_synonym: ChIP-SEQ evidence
comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used.
has_exact_synonym: ChIP-seq evidence
id: ECO:0000229
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: ChIP-seq evidence
database_cross_reference: PMID:22955991
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: OBI:0000716
database_cross_reference: PMID:22955991
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: definition

Axiom
annotated target: ChIP-SEQ evidence
database_cross_reference: OBI:0000716
annotated source: chromatin immunoprecipitation-seq evidence
annotated property: has_exact_synonym

chromatin immunoprecipitation-chip evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
creation date: 2010-11-16T05:43:55Z
comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp.
definition: A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions.
has_exact_synonym: ChIP-chip evidence
has_exact_synonym: ChIP-on-chip evidence
id: ECO:0000230
database_cross_reference: PSI-MI:MI:0225
has_obo_namespace: eco
created by: mchibucos

chromatin immunoprecipitation array
type: Axiom
annotated target: PSI-MI:MI:0225
annotated source: chromatin immunoprecipitation-chip evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:18388953
database_cross_reference: PMID:19381927
annotated source: chromatin immunoprecipitation-chip evidence
annotated property: definition

quantitative polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
creation date: 2010-11-16T05:57:20Z
definition: A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample.
id: ECO:0000231
has_exact_synonym: Q-PCR evidence
comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA. Although qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).
has_obo_namespace: eco
created by: mchibucos
has_related_synonym: qRT-PCR evidence
has_exact_synonym: quantitative PCR evidence
has_exact_synonym: real-time PCR evidence
has_exact_synonym: real-time polymerase chain reaction evidence

Axiom
annotated target: A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
annotated source: quantitative polymerase chain reaction evidence
annotated property: definition

chromosome conformation capture-based evidence
type: Class
subclass of: experimental genomic evidence
creation date: 2010-11-17T12:27:14Z
has_narrow_synonym: 3C
definition: A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation.
comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C.
id: ECO:0000232
has_narrow_synonym: chromosome conformation capture
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation.
database_cross_reference: ECO:MCC
annotated source: chromosome conformation capture-based evidence
annotated property: definition

chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
creation date: 2010-11-17T12:46:15Z
has_exact_synonym: 3C
comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation.
definition: A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step.
id: ECO:0000233
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step.
database_cross_reference: ECO:MCC
annotated source: chromosome conformation capture evidence
annotated property: definition

circularized chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
creation date: 2010-11-19T01:20:28Z
has_exact_synonym: 4C
comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation.
definition: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray.
id: ECO:0000234
has_exact_synonym: chromosome conformation capture on chip
has_exact_synonym: circularized 3C
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray.
database_cross_reference: ECO:MCC
annotated source: circularized chromosome conformation capture evidence
annotated property: definition

carbon-copy chromosome conformation capture evidence
type: Class
subclass of: chromosome conformation capture-based evidence
creation date: 2010-11-19T01:22:20Z
has_exact_synonym: 5C
comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation.
definition: A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation.
id: ECO:0000235
has_exact_synonym: carbon-copy 3C
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation.
database_cross_reference: ECO:MCC
annotated source: carbon-copy chromosome conformation capture evidence
annotated property: definition

chromosome conformation capture-PCR evidence
type: Class
subclass of: chromosome conformation capture evidence
creation date: 2010-11-19T03:00:47Z
has_broad_synonym: 3C
has_exact_synonym: 3C-PCR
definition: A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step.
id: ECO:0000236
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step.
database_cross_reference: ECO:MCC
annotated source: chromosome conformation capture-PCR evidence
annotated property: definition

chromosome conformation capture-qPCR evidence
type: Class
subclass of: chromosome conformation capture evidence
creation date: 2010-11-19T03:06:47Z
has_exact_synonym: 3C-qPCR
has_exact_synonym: 3C-quantitative PCR
has_exact_synonym: 3C-real-time PCR
definition: A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step.
id: ECO:0000237
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step.
database_cross_reference: ECO:MCC
annotated source: chromosome conformation capture-qPCR evidence
annotated property: definition

Hi-C evidence
type: Class
subclass of: chromosome conformation capture-based evidence
creation date: 2010-11-19T04:24:03Z
definition: A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation.
id: ECO:0000238
comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation.
database_cross_reference: ECO:MCC
annotated source: Hi-C evidence
annotated property: definition

chromosome conformation capture sequencing evidence
type: Class
subclass of: chromosome conformation capture-based evidence
creation date: 2010-11-30T03:22:25Z
has_exact_synonym: 3C sequencing
has_exact_synonym: 3C-seq
comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation.
has_exact_synonym: 4C sequencing
has_exact_synonym: 4C-seq
definition: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing.
id: ECO:0000239
has_exact_synonym: circularized 3C sequencing
has_exact_synonym: circularized 3C-seq
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing.
database_cross_reference: ECO:MCC
annotated source: chromosome conformation capture sequencing evidence
annotated property: definition

anatomical perturbation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2010-12-06T12:24:16Z
id: ECO:0000240
definition: Experimental phenotypic evidence where a structural feature of an organism is disrupted.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental phenotypic evidence where a structural feature of an organism is disrupted.
annotated source: anatomical perturbation evidence
annotated property: definition

environmental perturbation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2010-12-06T12:33:58Z
id: ECO:0000241
definition: Experimental phenotypic evidence where the extra-organismal context of an organism is modified.
comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth.
has_obo_namespace: eco
created by: mchibucos
has_related_synonym: mechanical constraint evidence

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental phenotypic evidence where the extra-organismal context of an organism is modified.
annotated source: environmental perturbation evidence
annotated property: definition

tissue ablation evidence
type: Class
subclass of: anatomical perturbation evidence
creation date: 2010-12-06T12:33:58Z
comment: A tissue ablation experiment involves observing the phenotype of an entity whose tissues have been disrupted through genetic or biochemical manipulation, physical destruction, or some other means.
definition: A type of anatomical perturbation that is based on disruption of tissue.
id: ECO:0000242
has_exact_synonym: ablated tissue evidence
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of anatomical perturbation that is based on disruption of tissue.
database_cross_reference: ECO:MCC
annotated source: tissue ablation evidence
annotated property: definition

tissue grafting evidence
type: Class
subclass of: anatomical perturbation evidence
creation date: 2010-12-06T12:33:58Z
definition: A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure.
id: ECO:0000243
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure.
database_cross_reference: ECO:MCC
annotated source: tissue grafting evidence
annotated property: definition

combinatorial evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: combinatorial evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-09T02:02:12Z
definition: A type of combinatorial analysis that is used in a manual assertion.
comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments.
id: ECO:0000244
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of combinatorial analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: combinatorial evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: combinatorial evidence used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

computational combinatorial evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: computational combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: computational combinatorial evidence
subclass of: combinatorial evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-09T02:37:54Z
definition: A type of computational combinatorial analysis that is used in a manual assertion.
id: ECO:0000245
database_cross_reference: GOECO:RCA
has_exact_synonym: RCA
has_obo_namespace: eco
has_exact_synonym: inferred from reviewed computational analysis
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
database_cross_reference: GOECO:RCA
annotated target: RCA
annotated source: computational combinatorial evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
database_cross_reference: GOECO:RCA
annotated target: inferred from reviewed computational analysis
annotated source: computational combinatorial evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: combinatorial evidence used in manual assertion
annotated source: computational combinatorial evidence used in manual assertion
annotated property: subClassOf

inferred from reviewed computational analysis
type: Axiom
annotated target: GOECO:RCA
annotated source: computational combinatorial evidence used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of computational combinatorial analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: computational combinatorial evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: computational combinatorial evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

computational combinatorial evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: computational combinatorial evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: computational combinatorial evidence
subclass of: combinatorial evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-09T02:37:54Z
definition: A type of computational combinatorial analysis that is used in an automatic assertion.
id: ECO:0000246
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of computational combinatorial analysis that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: combinatorial evidence used in automatic assertion
annotated source: computational combinatorial evidence used in automatic assertion
annotated property: subClassOf

sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-09T05:12:27Z
id: ECO:0000247
database_cross_reference: GOECO:ISA
has_exact_synonym: ISA
definition: Sequence alignment evidence that is used in a manual assertion.
has_obo_namespace: eco
has_exact_synonym: inferred from sequence alignment
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein

inferred from sequence alignment
type: Axiom
annotated target: GOECO:ISA
annotated source: sequence alignment evidence used in manual assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence alignment evidence that is used in a manual assertion.
annotated source: sequence alignment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:ISA
annotated target: ISA
annotated source: sequence alignment evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: sequence alignment evidence used in manual assertion
annotated target: sequence similarity evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: GOECO:ISA
annotated target: inferred from sequence alignment
annotated source: sequence alignment evidence used in manual assertion
annotated property: has_exact_synonym

sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-09T05:12:27Z
id: ECO:0000248
definition: Sequence alignment evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence alignment evidence that is used in an automatic assertion.
annotated source: sequence alignment evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated source: sequence alignment evidence used in automatic assertion
annotated target: sequence similarity evidence used in automatic assertion
annotated property: subClassOf

sequence similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence similarity evidence
subclass of: similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-09T05:22:30Z
id: ECO:0000249
definition: Sequence similarity evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: sequence similarity evidence used in automatic assertion
annotated target: similarity evidence used in automatic assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence similarity evidence that is used in an automatic assertion.
annotated source: sequence similarity evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: sequence similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

sequence similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence similarity evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-09T05:22:30Z
id: ECO:0000250
database_cross_reference: GOECO:ISS
definition: Sequence similarity evidence that is used in a manual assertion.
has_obo_namespace: eco
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence similarity evidence that is used in a manual assertion.
annotated source: sequence similarity evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: sequence similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: sequence similarity evidence used in manual assertion
annotated target: similarity evidence used in manual assertion
annotated property: subClassOf

inferred from sequence or structural similarity
type: Axiom
annotated target: GOECO:ISS
annotated source: sequence similarity evidence used in manual assertion
annotated property: database_cross_reference

similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: similarity evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-09T05:28:12Z
id: ECO:0000251
definition: Similarity evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated source: similarity evidence used in automatic assertion
annotated target: evidence used in automatic assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Similarity evidence that is used in an automatic assertion.
annotated source: similarity evidence used in automatic assertion
annotated property: definition

similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: similarity evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-09T05:28:12Z
id: ECO:0000252
definition: Similarity evidence that is used in a manual assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: similarity evidence used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Similarity evidence that is used in a manual assertion.
annotated source: similarity evidence used in manual assertion
annotated property: definition

genetic similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetic similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genetic similarity evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T01:42:44Z
id: ECO:0000253
definition: Genetic similarity evidence that is used in a manual assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: similarity evidence used in manual assertion
annotated source: genetic similarity evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Genetic similarity evidence that is used in a manual assertion.
annotated source: genetic similarity evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: genetic similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

genetic similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: genetic similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: genetic similarity evidence
subclass of: similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T01:42:44Z
id: ECO:0000254
definition: Genetic similarity evidence that is used in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Genetic similarity evidence that is used in an automatic assertion.
annotated source: genetic similarity evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: genetic similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: similarity evidence used in automatic assertion
annotated source: genetic similarity evidence used in automatic assertion
annotated property: subClassOf

match to sequence model evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: match to sequence model evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: match to sequence model evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T02:26:35Z
definition: A match to sequence model evidence that is employed in a manual assertion.
id: ECO:0000255
database_cross_reference: GOECO:ISM
database_cross_reference: GO_REF:0000011
has_obo_namespace: eco
created by: mchibucos

inferred from sequence model
type: Axiom
annotated target: GOECO:ISM
annotated source: match to sequence model evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: match to sequence model evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A match to sequence model evidence that is employed in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: match to sequence model evidence used in manual assertion
annotated property: definition

Hidden Markov Models (TIGR)
type: Axiom
annotated target: GO_REF:0000011
annotated source: match to sequence model evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated target: sequence similarity evidence used in manual assertion
annotated source: match to sequence model evidence used in manual assertion
annotated property: subClassOf

match to sequence model evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: match to sequence model evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: match to sequence model evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T02:26:35Z
definition: A match to sequence model evidence that is employed in an automatic assertion.
id: ECO:0000256
database_cross_reference: GO_REF:0000002
database_cross_reference: GO_REF:0000019
database_cross_reference: GO_REF:0000107
has_obo_namespace: eco
created by: mchibucos

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
type: Axiom
annotated target: GO_REF:0000019
annotated source: match to sequence model evidence used in automatic assertion
annotated property: database_cross_reference

Gene Ontology annotation through association of InterPro records with GO terms.
type: Axiom
annotated target: GO_REF:0000002
annotated source: match to sequence model evidence used in automatic assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: match to sequence model evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: sequence similarity evidence used in automatic assertion
annotated source: match to sequence model evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A match to sequence model evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: match to sequence model evidence used in automatic assertion
annotated property: definition

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl
type: Axiom
annotated target: GO_REF:0000107
annotated source: match to sequence model evidence used in automatic assertion
annotated property: database_cross_reference

motif similarity evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motif similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif similarity evidence
subclass of: match to sequence model evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T02:44:34Z
id: ECO:0000257
definition: Motif similarity evidence that is employed in a manual assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: motif similarity evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: match to sequence model evidence used in manual assertion
annotated source: motif similarity evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Motif similarity evidence that is employed in a manual assertion.
annotated source: motif similarity evidence used in manual assertion
annotated property: definition

motif similarity evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: motif similarity evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif similarity evidence
subclass of: match to sequence model evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T02:44:34Z
id: ECO:0000258
definition: Motif similarity evidence that is employed in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: motif similarity evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: match to sequence model evidence used in automatic assertion
annotated source: motif similarity evidence used in automatic assertion
annotated property: subClassOf

Axiom
database_cross_reference: ECO:MCC
annotated target: Motif similarity evidence that is employed in an automatic assertion.
annotated source: motif similarity evidence used in automatic assertion
annotated property: definition

match to InterPro member signature evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: match to InterPro member signature evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: match to InterPro member signature evidence
subclass of: match to sequence model evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T02:54:51Z
definition: A match to InterPro member signature evidence that is employed in an automatic assertion.
id: ECO:0000259
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: match to sequence model evidence used in automatic assertion
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
annotated target: A match to InterPro member signature evidence that is employed in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: match to InterPro member signature evidence used in automatic assertion
annotated property: definition

match to InterPro member signature evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: match to InterPro member signature evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: match to InterPro member signature evidence
subclass of: match to sequence model evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T02:54:51Z
definition: A match to InterPro member signature evidence that is employed in a manual assertion.
id: ECO:0000260
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A match to InterPro member signature evidence that is employed in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: match to sequence model evidence used in manual assertion
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: match to InterPro member signature evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

targeting sequence prediction evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: targeting sequence prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: targeting sequence prediction evidence
subclass of: motif similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T03:02:49Z
definition: A targeting sequence prediction that is employed in an automatic assertion.
id: ECO:0000261
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A targeting sequence prediction that is employed in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: motif similarity evidence used in automatic assertion
annotated source: targeting sequence prediction evidence used in automatic assertion
annotated property: subClassOf

targeting sequence prediction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: targeting sequence prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: targeting sequence prediction evidence
subclass of: motif similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T03:02:49Z
definition: A targeting sequence prediction that is employed in a manual assertion.
id: ECO:0000262
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: motif similarity evidence used in manual assertion
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A targeting sequence prediction that is employed in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: targeting sequence prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

transmembrane domain prediction evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: transmembrane domain prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: transmembrane domain prediction evidence
subclass of: motif similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T03:28:35Z
id: ECO:0000263
definition: Transmembrane domain prediction that is employed in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: motif similarity evidence used in automatic assertion
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
database_cross_reference: ECO:MCC
annotated target: Transmembrane domain prediction that is employed in an automatic assertion.
annotated source: transmembrane domain prediction evidence used in automatic assertion
annotated property: definition

transmembrane domain prediction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transmembrane domain prediction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transmembrane domain prediction evidence
subclass of: motif similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T03:28:35Z
id: ECO:0000264
definition: Transmembrane domain prediction that is employed in a manual assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Transmembrane domain prediction that is employed in a manual assertion.
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: motif similarity evidence used in manual assertion
annotated source: transmembrane domain prediction evidence used in manual assertion
annotated property: subClassOf

sequence orthology evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: sequence orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence orthology evidence
subclass of: sequence similarity evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2010-12-10T03:39:41Z
id: ECO:0000265
database_cross_reference: GO_REF:0000003
database_cross_reference: GO_REF:0000019
database_cross_reference: GO_REF:0000035
has_related_synonym: Ortholog evidence
definition: Sequence orthology evidence that is employed in an automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: sequence orthology evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
type: Axiom
annotated target: GO_REF:0000019
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated target: sequence similarity evidence used in automatic assertion
annotated source: sequence orthology evidence used in automatic assertion
annotated property: subClassOf

Gene Ontology annotation based on Enzyme Commission mapping.
type: Axiom
annotated target: GO_REF:0000003
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference

Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara
type: Axiom
annotated target: GO_REF:0000035
annotated source: sequence orthology evidence used in automatic assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence orthology evidence that is employed in an automatic assertion.
annotated source: sequence orthology evidence used in automatic assertion
annotated property: definition

sequence orthology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: sequence orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence orthology evidence
subclass of: sequence similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-10T03:39:41Z
id: ECO:0000266
database_cross_reference: GOECO:ISO
has_related_synonym: Ortholog evidence
definition: Sequence orthology evidence that is employed in a manual assertion.
has_obo_namespace: eco
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
is inferred: true
annotated target: sequence similarity evidence used in manual assertion
annotated source: sequence orthology evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: sequence orthology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: ECO:MCC
annotated target: Sequence orthology evidence that is employed in a manual assertion.
annotated source: sequence orthology evidence used in manual assertion
annotated property: definition

inferred from sequence orthology
type: Axiom
annotated target: GOECO:ISO
annotated source: sequence orthology evidence used in manual assertion
annotated property: database_cross_reference

enzyme-linked immunoabsorbent assay evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2010-12-14T01:57:13Z
definition: A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate.
id: ECO:0000267
has_exact_synonym: ELISA evidence
comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein.
database_cross_reference: PSI-MI:MI:0411
has_obo_namespace: eco
has_narrow_synonym: enzyme-linked immunosorbent assay
created by: mchibucos

Axiom
annotated target: A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate.
database_cross_reference: ECO:MCC
database_cross_reference: ECO:SW
database_cross_reference: PMID:23949770
annotated source: enzyme-linked immunoabsorbent assay evidence
annotated property: definition

enzyme linked immunosorbent assay
type: Axiom
annotated target: PSI-MI:MI:0411
annotated source: enzyme-linked immunoabsorbent assay evidence
annotated property: database_cross_reference

flow cytometry evidence
type: Class
subclass of: direct assay evidence
creation date: 2010-12-14T02:07:08Z
definition: A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles.
id: ECO:0000268
has_exact_synonym: FCM
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles.
database_cross_reference: ECO:MCC
annotated source: flow cytometry evidence
annotated property: definition

experimental evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: experimental evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:36:49Z
definition: A type of experimental evidence that is used in a manual assertion.
id: ECO:0000269
database_cross_reference: GOECO:EXP
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: experimental evidence used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

inferred from experiment
type: Axiom
annotated target: GOECO:EXP
annotated source: experimental evidence used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of experimental evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: experimental evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: experimental evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression pattern evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:46:39Z
definition: A type of expression pattern evidence that is used in a manual assertion.
id: ECO:0000270
database_cross_reference: GOECO:IEP
has_obo_namespace: eco
created by: mchibucos

inferred from expression pattern
type: Axiom
annotated target: GOECO:IEP
annotated source: expression evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of expression pattern evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: expression evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: expression evidence used in manual assertion
annotated property: subClassOf

array experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: array experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: array experiment evidence
subclass of: expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:51:58Z
definition: A type of array experiment evidence that is used in a manual assertion.
id: ECO:0000271
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of array experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: array experiment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: array experiment evidence used in manual assertion
annotated property: subClassOf

Affymetrix array experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Affymetrix array experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Affymetrix array experiment evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:53:36Z
definition: A type of Affymetrix array experiment evidence that is used in a manual assertion.
id: ECO:0000272
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of Affymetrix array experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: Affymetrix array experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

cDNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cDNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: cDNA microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:55:49Z
definition: A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion.
id: ECO:0000273
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cDNA microarray evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: cDNA microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: cDNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

CpG island microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: CpG island microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: CpG island microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T05:58:44Z
definition: A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion.
id: ECO:0000274
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: CpG island microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: CpG island microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: CpG island microarray evidence used in manual assertion
annotated property: definition

expression microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:01:26Z
definition: A type of expression microarray evidence that is used in a manual assertion.
id: ECO:0000275
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: expression microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of expression microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: expression microarray evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: expression microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

genomic microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genomic microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genomic microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:03:28Z
definition: A type of evidence arising from a genomic microarray experiment that is used in a manual assertion.
id: ECO:0000276
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: genomic microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: genomic microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of evidence arising from a genomic microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: genomic microarray evidence used in manual assertion
annotated property: definition

Nimblegen array evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Nimblegen array evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Nimblegen array evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:05:20Z
definition: A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion.
id: ECO:0000277
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Nimblegen array evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: Nimblegen array evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Nimblegen array evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

oligonucleotide microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: oligonucleotide microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: oligonucleotide microarray evidence
subclass of: array experiment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:09:45Z
definition: A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion.
id: ECO:0000278
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: oligonucleotide microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

western blot evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: western blot evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: western blot evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:20:01Z
definition: A type of Western blot assay that is used in a manual assertion.
id: ECO:0000279
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: western blot evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of Western blot assay that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: western blot evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: western blot evidence used in manual assertion
annotated target: direct assay evidence used in manual assertion
annotated property: subClassOf

protein expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: protein expression level evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:21:54Z
definition: A type of protein expression level analysis that is used in a manual assertion.
id: ECO:0000280
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: protein expression level evidence used in manual assertion
annotated target: protein expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of protein expression level analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: protein expression level evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: protein expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

expression library screen evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: expression library screen evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression library screen evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:24:05Z
definition: A type of expression library screen evidence that is used in a manual assertion.
id: ECO:0000281
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: expression library screen evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: expression library screen evidence used in manual assertion
annotated target: protein expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of expression library screen evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: expression library screen evidence used in manual assertion
annotated property: definition

heterologous protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: heterologous protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: heterologous protein expression evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:27:04Z
definition: A type of heterologous protein expression analysis that is used in a manual assertion.
id: ECO:0000282
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: heterologous protein expression evidence used in manual assertion
annotated target: protein expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of heterologous protein expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: heterologous protein expression evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: heterologous protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

spatial pattern of protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spatial pattern of protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: spatial pattern of protein expression evidence
subclass of: protein expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:31:44Z
definition: A type of protein expression spatial pattern analysis that is used in a manual assertion.
id: ECO:0000283
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated target: protein expression evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of protein expression spatial pattern analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: spatial pattern of protein expression evidence used in manual assertion
annotated property: definition

protein expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: protein expression evidence
subclass of: expression evidence used in manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:33:46Z
definition: A type of protein expression analysis that is used in a manual assertion.
id: ECO:0000284
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: protein expression evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: protein expression evidence used in manual assertion
annotated target: direct assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of protein expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: protein expression evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: protein expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

microarray RNA expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: microarray RNA expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microarray RNA expression level evidence
subclass of: expression microarray evidence used in manual assertion
subclass of: transcript expression level evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:41:33Z
definition: A type of microarray RNA expression level data that is used in manual assertion.
id: ECO:0000285
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: microarray RNA expression level evidence used in manual assertion
annotated target: transcript expression level evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: expression microarray evidence used in manual assertion
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of microarray RNA expression level data that is used in manual assertion.
database_cross_reference: ECO:MCC
annotated source: microarray RNA expression level evidence used in manual assertion
annotated property: definition

transcript expression level evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transcript expression level evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression level evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:52:03Z
definition: A type of transcript expression level data that is used in manual assertion.
id: ECO:0000286
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: transcript expression level evidence used in manual assertion
annotated target: transcript expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of transcript expression level data that is used in manual assertion.
database_cross_reference: ECO:MCC
annotated source: transcript expression level evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: transcript expression level evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

differential hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: differential hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: differential hybridization evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:55:14Z
definition: A type of differential hybridization experiment that is used in a manual assertion.
id: ECO:0000287
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: differential hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of differential hybridization experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: differential hybridization evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: differential hybridization evidence used in manual assertion
annotated target: transcript expression evidence used in manual assertion
annotated property: subClassOf

RNA protection assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA protection assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: RNA protection assay evidence
subclass of: nuclease protection assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:57:33Z
definition: A type of RNA protection assay that is used in a manual assertion.
id: ECO:0000288
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of RNA protection assay that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: RNA protection assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: RNA protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: RNA protection assay evidence used in manual assertion
annotated target: nuclease protection assay evidence used in manual assertion
annotated property: subClassOf

spatial pattern of transcript expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spatial pattern of transcript expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: spatial pattern of transcript expression evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T06:59:18Z
definition: A type of spatial pattern of transcript expression analysis that is used in a manual assertion.
id: ECO:0000289
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated target: transcript expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of spatial pattern of transcript expression analysis that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: spatial pattern of transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

subtractive hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: subtractive hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: subtractive hybridization evidence
subclass of: transcript expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T07:00:48Z
definition: A type of subtractive hybridization experiment that is used in a manual assertion.
id: ECO:0000290
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: subtractive hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: subtractive hybridization evidence used in manual assertion
annotated target: transcript expression evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of subtractive hybridization experiment that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: subtractive hybridization evidence used in manual assertion
annotated property: definition

transcript expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transcript expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression evidence
subclass of: expression evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2010-12-20T07:02:08Z
definition: A type of transcript expression evidence that is used in a manual assertion.
id: ECO:0000291
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of transcript expression evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: transcript expression evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: transcript expression evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: transcript expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

morpholino experiment evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2011-01-10T03:36:25Z
definition: A type of experimental phenotypic evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell.
id: ECO:0000292
comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method.
has_broad_synonym: anti-sense
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of experimental phenotypic evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell.
database_cross_reference: ECO:MCC
annotated source: morpholino experiment evidence
annotated property: definition

systematic evolution of ligands by exponential amplification evidence
type: Class
subclass of: physical interaction evidence
creation date: 2011-01-10T04:23:50Z
definition: A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand.
id: ECO:0000293
database_cross_reference: PSI-MI:MI:0657
comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein.
has_exact_synonym: SELEX evidence
has_obo_namespace: eco
has_related_synonym: in vitro evolution evidence
has_exact_synonym: in vitro selection evidence
created by: mchibucos

Axiom
annotated target: A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand.
database_cross_reference: ECO:MCC
database_cross_reference: url:http://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment
annotated source: systematic evolution of ligands by exponential amplification evidence
annotated property: definition

systematic evolution of ligands by exponential enrichment
type: Axiom
annotated target: PSI-MI:MI:0657
annotated source: systematic evolution of ligands by exponential amplification evidence
annotated property: database_cross_reference

bacterial one-hybrid evidence
type: Class
subclass of: hybrid interaction evidence
creation date: 2011-01-10T06:16:20Z
definition: A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey).
has_exact_synonym: B1H evidence
comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes.
id: ECO:0000294
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey).
database_cross_reference: ECO:MCC
annotated source: bacterial one-hybrid evidence
annotated property: definition

RNA-seq evidence
type: Class
subclass of: transcript expression evidence
creation date: 2011-01-11T12:54:50Z
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules.
id: ECO:0000295
has_related_synonym: RNA sequencing
comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.
has_exact_synonym: WTSS
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: whole transcriptome shotgun sequencing

Axiom
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules.
database_cross_reference: ECO:MCC
annotated source: RNA-seq evidence
annotated property: definition

Axiom
database_cross_reference: PMID:18611170
annotated target: whole transcriptome shotgun sequencing
annotated source: RNA-seq evidence
annotated property: has_exact_synonym

green fluorescent protein transcript localization evidence
type: Class
subclass of: transcriptional activation assay
creation date: 2011-01-11T02:31:19Z
definition: A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene.
id: ECO:0000296
comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of green fluorescent protein".
has_obo_namespace: eco
has_exact_synonym: localization of GFP transcript
created by: mchibucos

Axiom
annotated target: A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene.
database_cross_reference: ECO:MCC
annotated source: green fluorescent protein transcript localization evidence
annotated property: definition

LacZ transcript localization evidence
type: Class
subclass of: transcriptional activation assay
creation date: 2011-01-11T02:53:19Z
definition: A transcriptional activation assay that uses a LacZ reporter gene.
id: ECO:0000297
comment: This term describes transcriptional activation; to describe immunolocalization of the protein, use the term "immunolocalization of LacZ protein".
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A transcriptional activation assay that uses a LacZ reporter gene.
database_cross_reference: ECO:MCC
annotated source: LacZ transcript localization evidence
annotated property: definition

cleavage arrested development evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2011-01-11T03:28:49Z
definition: A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked.
id: ECO:0000298
comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked.
database_cross_reference: ECO:MCC
annotated source: cleavage arrested development evidence
annotated property: definition

cytochalasin experiment evidence
type: Class
subclass of: cleavage arrested development evidence
creation date: 2011-01-11T03:30:29Z
definition: A type of cleavage arrested development that arises after treatment with cytochalasin.
comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments.
id: ECO:0000299
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of cleavage arrested development that arises after treatment with cytochalasin.
database_cross_reference: ECO:MCC
annotated source: cytochalasin experiment evidence
annotated property: definition

green fluorescent protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
creation date: 2011-01-11T03:48:50Z
definition: A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker.
id: ECO:0000300
has_exact_synonym: GFP immunolocalization evidence
comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript".
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker.
database_cross_reference: ECO:MCC
annotated source: green fluorescent protein immunolocalization evidence
annotated property: definition

beta-galactosidase protein immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
creation date: 2011-01-11T04:06:40Z
definition: A type of immunolocalization evidence that was generated using LacZ protein as a marker.
id: ECO:0000301
has_exact_synonym: LacZ protein immunolocalization evidence
comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript".
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of immunolocalization evidence that was generated using LacZ protein as a marker.
database_cross_reference: ECO:MCC
annotated source: beta-galactosidase protein immunolocalization evidence
annotated property: definition

author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: author statement
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:26:47Z
definition: A type of author statement that is used in a manual assertion.
id: ECO:0000302
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: author statement used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: author statement used in manual assertion
annotated property: definition

non-traceable author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: non-traceable author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: non-traceable author statement
subclass of: author statement used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:29:50Z
definition: A type of non-traceable author statement that is used in a manual assertion.
id: ECO:0000303
database_cross_reference: GOECO:NAS
has_exact_synonym: NAS
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: non-traceable author statement

Axiom
database_cross_reference: GOECO:NAS
annotated target: non-traceable author statement
annotated source: non-traceable author statement used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: author statement used in manual assertion
annotated source: non-traceable author statement used in manual assertion
annotated property: subClassOf

non-traceable author statement
type: Axiom
annotated target: GOECO:NAS
annotated source: non-traceable author statement used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of non-traceable author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: non-traceable author statement used in manual assertion
annotated property: definition

Axiom
database_cross_reference: GOECO:NAS
annotated target: NAS
annotated source: non-traceable author statement used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: non-traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

traceable author statement used in manual assertion
type: Class
equivalent class: Class
  intersection of: traceable author statement
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: traceable author statement
subclass of: author statement used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:31:03Z
definition: A type of traceable author statement that is used in a manual assertion.
id: ECO:0000304
database_cross_reference: GOECO:TAS
has_exact_synonym: TAS
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: traceable author statement

traceable author statement
type: Axiom
annotated target: GOECO:TAS
annotated source: traceable author statement used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of traceable author statement that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: traceable author statement used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: traceable author statement used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:TAS
annotated target: traceable author statement
annotated source: traceable author statement used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: author statement used in manual assertion
annotated source: traceable author statement used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: GOECO:TAS
annotated target: TAS
annotated source: traceable author statement used in manual assertion
annotated property: has_exact_synonym

curator inference used in manual assertion
type: Class
equivalent class: Class
  intersection of: curator inference
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: curator inference
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:33:32Z
definition: A type of curator inference that is used in a manual assertion.
id: ECO:0000305
database_cross_reference: GOECO:IC
has_obo_namespace: eco
created by: mchibucos
in_subset: valid with biological process
in_subset: valid with cellular component
in_subset: valid with molecular function

Axiom
annotated target: A type of curator inference that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: curator inference used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: curator inference used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

inferred by curator
type: Axiom
annotated target: GOECO:IC
annotated source: curator inference used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: curator inference used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

inference from background scientific knowledge used in manual assertion
type: Class
equivalent class: Class
  intersection of: inference from background scientific knowledge
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: inference from background scientific knowledge
subclass of: curator inference used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:35:11Z
definition: A type of inference from background scientific knowledge that is used in a manual assertion.
id: ECO:0000306
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: curator inference used in manual assertion
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of inference from background scientific knowledge that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: inference from background scientific knowledge used in manual assertion
annotated property: definition

no biological data found used in manual assertion
type: Class
equivalent class: Class
  intersection of: no biological data found
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: no biological data found
subclass of: curator inference used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-01-20T02:43:18Z
definition: A type of no biological data found that is used in a manual assertion.
id: ECO:0000307
database_cross_reference: GOECO:ND
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: curator inference used in manual assertion
annotated source: no biological data found used in manual assertion
annotated property: subClassOf

no biological data available
type: Axiom
annotated target: GOECO:ND
annotated source: no biological data found used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: no biological data found used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of no biological data found that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: no biological data found used in manual assertion
annotated property: definition

biological aspect of ancestor evidence
type: Class
subclass of: phylogenetic evidence
creation date: 2011-02-04T05:41:26Z
definition: A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene.
id: ECO:0000308
comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene.
database_cross_reference: ECO:MCC
annotated source: biological aspect of ancestor evidence
annotated property: definition

cap analysis of gene expression evidence
type: Class
subclass of: transcript expression evidence
creation date: 2011-02-17T01:33:09Z
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system.
has_exact_synonym: CAGE
comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed.
id: ECO:0000309
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: CAGE
database_cross_reference: PMID:16489339
annotated source: cap analysis of gene expression evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system.
database_cross_reference: ECO:MCC
annotated source: cap analysis of gene expression evidence
annotated property: definition

nano-cap analysis of gene expression evidence
type: Class
subclass of: transcript expression evidence
creation date: 2011-02-17T01:33:34Z
definition: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching.
id: ECO:0000310
comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA.
has_obo_namespace: eco
created by: mchibucos
has_related_synonym: nanoCAGE

Axiom
annotated target: A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching.
database_cross_reference: ECO:MCC
annotated source: nano-cap analysis of gene expression evidence
annotated property: definition

Axiom
database_cross_reference: PMID:20543846
annotated target: nanoCAGE
annotated source: nano-cap analysis of gene expression evidence
annotated property: has_related_synonym

imported information
type: Class
subclass of: evidence
creation date: 2011-03-02T05:24:13Z
definition: An evidence type that is based on work performed by a person or group prior to a use by different person or group.
id: ECO:0000311
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: An evidence type that is based on work performed by a person or group prior to a use by different person or group.
database_cross_reference: ECO:MCC
annotated source: imported information
annotated property: definition

imported information used in manual assertion
type: Class
equivalent class: Class
  intersection of: imported information
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: imported information
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-03-02T05:29:44Z
definition: A type of imported information that is used in a manual assertion.
id: ECO:0000312
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: imported information used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of imported information that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: imported information used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: imported information used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

imported information used in automatic assertion
type: Class
equivalent class: Class
  intersection of: imported information
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: imported information
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2011-03-02T05:33:43Z
definition: A type of imported information that is used in an automatic assertion.
id: ECO:0000313
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of imported information that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: imported information used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: imported information used in automatic assertion
annotated target: evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: imported information used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

direct assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: direct assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T04:59:31Z
definition: A type of direct assay evidence that is used in a manual assertion.
id: ECO:0000314
database_cross_reference: GOECO:IDA
has_related_synonym: IDA
has_obo_namespace: eco
has_exact_synonym: inferred from direct assay
created by: mchibucos
in_subset: valid with chemical entity

Axiom
is inferred: true
annotated source: direct assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IDA
annotated target: IDA
annotated source: direct assay evidence used in manual assertion
annotated property: has_related_synonym

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: direct assay evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: GOECO:IDA
annotated target: inferred from direct assay
annotated source: direct assay evidence used in manual assertion
annotated property: has_exact_synonym

inferred from direct assay
type: Axiom
annotated target: GOECO:IDA
annotated source: direct assay evidence used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of direct assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: direct assay evidence used in manual assertion
annotated property: definition

mutant phenotype evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mutant phenotype evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: mutant phenotype evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:12:49Z
definition: A type of mutant phenotype evidence that is used in a manual assertion.
id: ECO:0000315
database_cross_reference: GOECO:IMP
has_related_synonym: IMP
has_obo_namespace: eco
has_related_synonym: inferred from mutant phenotype
created by: mchibucos
in_subset: valid with chemical entity
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: mutant phenotype evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: GOECO:IMP
annotated target: inferred from mutant phenotype
annotated source: mutant phenotype evidence used in manual assertion
annotated property: has_related_synonym

Axiom
is inferred: true
annotated source: mutant phenotype evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IMP
annotated target: IMP
annotated source: mutant phenotype evidence used in manual assertion
annotated property: has_related_synonym

inferred from mutant phenotype
type: Axiom
annotated target: GOECO:IMP
annotated source: mutant phenotype evidence used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of mutant phenotype evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: mutant phenotype evidence used in manual assertion
annotated property: definition

genetic interaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genetic interaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genetic interaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:14:42Z
definition: A type of genetic interaction experiment evidence that is used in a manual assertion.
id: ECO:0000316
database_cross_reference: GOECO:IGI
has_exact_synonym: IGI
has_obo_namespace: eco
has_exact_synonym: inferred from genetic interaction
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
is inferred: true
annotated source: genetic interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of genetic interaction experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: genetic interaction evidence used in manual assertion
annotated property: definition

Axiom
database_cross_reference: GOECO:IGI
annotated target: inferred from genetic interaction
annotated source: genetic interaction evidence used in manual assertion
annotated property: has_exact_synonym

inferred from genetic interaction
type: Axiom
annotated target: GOECO:IGI
annotated source: genetic interaction evidence used in manual assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: GOECO:IGI
annotated target: IGI
annotated source: genetic interaction evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: genetic interaction evidence used in manual assertion
annotated property: subClassOf

genomic context evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: genomic context evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: genomic context evidence
subclass of: evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:22:53Z
definition: A type of genomic context evidence that is used in a manual assertion.
id: ECO:0000317
database_cross_reference: GOECO:IGC
has_related_synonym: IGC
has_obo_namespace: eco
has_exact_synonym: inferred from genomic context
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein

Axiom
database_cross_reference: GOECO:IGC
annotated target: IGC
annotated source: genomic context evidence used in manual assertion
annotated property: has_related_synonym

inferred from genomic context
type: Axiom
annotated target: GOECO:IGC
annotated source: genomic context evidence used in manual assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of genomic context evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: genomic context evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: genomic context evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IGC
annotated target: inferred from genomic context
annotated source: genomic context evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: genomic context evidence used in manual assertion
annotated target: evidence used in manual assertion
annotated property: subClassOf

biological aspect of ancestor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological aspect of ancestor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: similarity evidence used in manual assertion
subclass of: biological aspect of ancestor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:23:51Z
definition: A type of biological aspect of ancestor evidence that is used in a manual assertion.
id: ECO:0000318
database_cross_reference: GOECO:IBA
has_exact_synonym: IBA
has_obo_namespace: eco
has_exact_synonym: inferred from biological aspect of ancestor
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated target: A type of biological aspect of ancestor evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: similarity evidence used in manual assertion
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IBA
annotated target: IBA
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
database_cross_reference: GOECO:IBA
annotated target: inferred from biological aspect of ancestor
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: has_exact_synonym

inferred from biological aspect of ancestor
type: Axiom
annotated target: GOECO:IBA
annotated source: biological aspect of ancestor evidence used in manual assertion
annotated property: database_cross_reference

biological aspect of descendant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological aspect of descendant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: biological aspect of descendant evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:25:09Z
definition: A type of biological aspect of descendant evidence that is used in a manual assertion.
id: ECO:0000319
database_cross_reference: GOECO:IBD
has_exact_synonym: IBD
has_obo_namespace: eco
has_exact_synonym: inferred from biological aspect of descendant
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated target: A type of biological aspect of descendant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: definition

inferred from biological aspect of descendant
type: Axiom
annotated target: GOECO:IBD
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: GOECO:IBD
annotated target: inferred from biological aspect of descendant
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IBD
annotated target: IBD
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: similarity evidence used in manual assertion
annotated source: biological aspect of descendant evidence used in manual assertion
annotated property: subClassOf

phylogenetic determination of loss of key residues evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phylogenetic determination of loss of key residues evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: phylogenetic determination of loss of key residues evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:25:48Z
definition: A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion.
id: ECO:0000320
database_cross_reference: GOECO:IKR
database_cross_reference: GOECO:IMR
has_exact_synonym: IKR
has_exact_synonym: IMR
has_obo_namespace: eco
has_exact_synonym: inferred from key residues
has_exact_synonym: inferred from missing residues
created by: mchibucos

inferred from missing residues
type: Axiom
annotated target: GOECO:IMR
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: GOECO:IKR
annotated target: IKR
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: similarity evidence used in manual assertion
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: subClassOf

Axiom
database_cross_reference: GOECO:IKR
annotated target: inferred from key residues
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
annotated target: A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: definition

Axiom
database_cross_reference: GOECO:IKR
annotated target: IMR
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
database_cross_reference: GOECO:IKR
annotated target: inferred from missing residues
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: has_exact_synonym

inferred from key residues
type: Axiom
annotated target: GOECO:IKR
annotated source: phylogenetic determination of loss of key residues evidence used in manual assertion
annotated property: database_cross_reference

rapid divergence from ancestral sequence evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: rapid divergence from ancestral sequence evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: rapid divergence from ancestral sequence evidence
subclass of: similarity evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2011-10-28T05:26:42Z
definition: A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion.
id: ECO:0000321
database_cross_reference: GOECO:IRD
has_exact_synonym: IRD
has_obo_namespace: eco
has_exact_synonym: inferred from rapid divergence
created by: mchibucos
in_subset: valid with gene
in_subset: valid with protein

Axiom
annotated target: A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: definition

Axiom
database_cross_reference: GOECO:IRD
annotated target: IRD
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: has_exact_synonym

inferred from rapid divergence
type: Axiom
annotated target: GOECO:IRD
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
database_cross_reference: GOECO:IRD
annotated target: inferred from rapid divergence
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated target: similarity evidence used in manual assertion
annotated source: rapid divergence from ancestral sequence evidence used in manual assertion
annotated property: subClassOf

imported manually asserted information used in automatic assertion
type: Class
subclass of: imported information used in automatic assertion
creation date: 2011-12-13T12:04:48Z
id: ECO:0000322
definition: Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
database_cross_reference: GO_REF:0000041
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
annotated source: imported manually asserted information used in automatic assertion
annotated property: definition

IEA (UniProt UniPathway)
type: Axiom
annotated target: GO_REF:0000041
annotated source: imported manually asserted information used in automatic assertion
annotated property: database_cross_reference

imported automatically asserted information used in automatic assertion
type: Class
subclass of: imported information used in automatic assertion
creation date: 2011-12-13T02:06:25Z
id: ECO:0000323
definition: Evidence that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
has_obo_namespace: eco
created by: mchibucos

Axiom
database_cross_reference: ECO:MCC
annotated target: Evidence that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion.
annotated source: imported automatically asserted information used in automatic assertion
annotated property: definition

imaging assay evidence
type: Class
subclass of: direct assay evidence
creation date: 2012-02-23T12:01:43Z
definition: Direct assay evidence derived from analysis of an image.
id: ECO:0000324
has_alternative_id: ECO:0001122
has_obo_namespace: eco
created by: mchibucos
has_narrow_synonym: radiologic test evidence

Axiom
annotated target: Direct assay evidence derived from analysis of an image.
database_cross_reference: ECO:MCC
annotated source: imaging assay evidence
annotated property: definition

chromatography evidence
type: Class
subclass of: experimental evidence
creation date: 2012-05-16T11:46:27Z
definition: A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase.
id: ECO:0000325
has_exact_synonym: chromatographic evidence
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase.
database_cross_reference: ECO:MCC
annotated source: chromatography evidence
annotated property: definition

transcript splice pattern evidence
type: Class
subclass of: sequence alignment evidence
creation date: 2012-06-08T11:03:59Z
definition: A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript.
id: ECO:0000326
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript.
database_cross_reference: ECO:MCC
annotated source: transcript splice pattern evidence
annotated property: definition

whole transcript splice pattern evidence
type: Class
subclass of: transcript splice pattern evidence
creation date: 2012-06-08T11:11:24Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features.
id: ECO:0000327
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features.
database_cross_reference: ECO:MCC
annotated source: whole transcript splice pattern evidence
annotated property: definition

coding sequence splice pattern evidence
type: Class
subclass of: transcript splice pattern evidence
creation date: 2012-06-08T11:21:57Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript.
id: ECO:0000328
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript.
database_cross_reference: ECO:MCC
annotated source: coding sequence splice pattern evidence
annotated property: definition

whole transcript splice pattern evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: whole transcript splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: whole transcript splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2012-06-08T11:40:03Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion.
id: ECO:0000329
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: sequence alignment evidence used in manual assertion
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion.
database_cross_reference: ECO:MCC
annotated source: whole transcript splice pattern evidence used in manual assertion
annotated property: definition

coding sequence splice pattern evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: coding sequence splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: coding sequence splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2012-06-08T11:43:47Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion.
id: ECO:0000330
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: sequence alignment evidence used in manual assertion
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion.
database_cross_reference: ECO:MCC
annotated source: coding sequence splice pattern evidence used in manual assertion
annotated property: definition

coding sequence splice pattern evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: coding sequence splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: coding sequence splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2012-06-08T11:48:09Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion.
id: ECO:0000331
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion.
database_cross_reference: ECO:MCC
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated target: sequence alignment evidence used in automatic assertion
annotated source: coding sequence splice pattern evidence used in automatic assertion
annotated property: subClassOf

whole transcript splice pattern evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: whole transcript splice pattern evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: whole transcript splice pattern evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2012-06-08T11:49:17Z
definition: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion.
id: ECO:0000332
comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child.
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: sequence alignment evidence used in automatic assertion
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
annotated target: A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion.
database_cross_reference: ECO:MCC
annotated source: whole transcript splice pattern evidence used in automatic assertion
annotated property: definition

sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
creation date: 2012-07-31T02:56:43Z
definition: A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility.
id: ECO:0000333
comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size.
has_exact_synonym: SDS-PAGE evidence
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility.
database_cross_reference: ECO:MCC
database_cross_reference: PubMed:4861258
database_cross_reference: url:http://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html
annotated source: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
annotated property: definition

particle size and count assay evidence
type: Class
subclass of: direct assay evidence
creation date: 2012-07-31T03:19:32Z
definition: A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined.
comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system.
id: ECO:0000334
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined.
database_cross_reference: ECO:MCC
annotated source: particle size and count assay evidence
annotated property: definition

substance quantification evidence
type: Class
subclass of: direct assay evidence
creation date: 2012-07-31T03:52:46Z
definition: A type of direct assay evidence where the quantity of a substance used as part of an assay is measured.
id: ECO:0000335
comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of direct assay evidence where the quantity of a substance used as part of an assay is measured.
database_cross_reference: ECO:MCC
annotated source: substance quantification evidence
annotated property: definition

competitive growth assay evidence
type: Class
subclass of: biological assay evidence
creation date: 2012-08-24T12:03:58Z
definition: A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed.
id: ECO:0000336
has_obo_namespace: eco
has_exact_synonym: fitness profiling
created by: mchibucos

Axiom
database_cross_reference: GOC:MAH
database_cross_reference: PMID:20537132
annotated target: fitness profiling
annotated source: competitive growth assay evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed.
database_cross_reference: GOC:MAH
database_cross_reference: PMID:14718172
database_cross_reference: PMID:20537132
annotated source: competitive growth assay evidence
annotated property: definition

gel electrophoresis evidence
type: Class
subclass of: direct assay evidence
creation date: 2012-08-24T12:11:51Z
definition: A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field.
id: ECO:0000337
comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field.
database_cross_reference: ECO:MCC
database_cross_reference: wikipedia:Gel_electrophoresis
annotated source: gel electrophoresis evidence
annotated property: definition

pulsed-field gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
creation date: 2012-08-24T12:34:01Z
id: ECO:0000338
definition: Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel.
has_exact_synonym: PFGE
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel.
database_cross_reference: PMID:6373014
annotated source: pulsed-field gel electrophoresis evidence
annotated property: definition

two-dimensional agarose gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
has_exact_synonym: 2-D agarose gel electrophoresis
creation date: 2012-08-24T12:42:37Z
definition: A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide.
id: ECO:0000339
comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide.
database_cross_reference: PMID:16118435
database_cross_reference: PMID:8594382
annotated source: two-dimensional agarose gel electrophoresis evidence
annotated property: definition

plasmid maintenance assay evidence
type: Class
subclass of: experimental evidence
creation date: 2012-08-24T01:27:58Z
id: ECO:0000340
definition: Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated.
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: minichromosome maintenance assay evidence

Axiom
database_cross_reference: ECO:MCC
annotated target: Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated.
database_cross_reference: PMID:6323245
annotated source: plasmid maintenance assay evidence
annotated property: definition

Axiom
database_cross_reference: PMID:6323245
annotated target: minichromosome maintenance assay evidence
annotated source: plasmid maintenance assay evidence
annotated property: has_exact_synonym

specific protein inhibition by antibody evidence
type: Class
subclass of: specific protein inhibition evidence
creation date: 2012-08-24T01:48:24Z
definition: A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody.
id: ECO:0000341
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody.
database_cross_reference: ECO:MCC
annotated source: specific protein inhibition by antibody evidence
annotated property: definition

Support of intron position by RNA-seq alignment evidence
type: Class
subclass of: RNA-seq evidence
creation date: 2013-04-03T04:22:05Z
definition: A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.
id: ECO:0000342
comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.
database_cross_reference: ECO:MCC
annotated source: Support of intron position by RNA-seq alignment evidence
annotated property: definition

Full support of intron position by RNA-seq alignment evidence
type: Class
subclass of: Support of intron position by RNA-seq alignment evidence
creation date: 2013-04-03T04:41:48Z
definition: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript.
id: ECO:0000343
comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript.
database_cross_reference: ECO:MCC
annotated source: Full support of intron position by RNA-seq alignment evidence
annotated property: definition

Partial support of intron position by RNA-seq alignment evidence
type: Class
subclass of: Support of intron position by RNA-seq alignment evidence
creation date: 2013-04-03T04:50:46Z
definition: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript.
id: ECO:0000344
comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript.
database_cross_reference: ECO:MCC
annotated source: Partial support of intron position by RNA-seq alignment evidence
annotated property: definition

single exon transcript confirmation via alignment evidence
type: Class
subclass of: sequence alignment evidence
creation date: 2013-04-03T05:10:34Z
id: ECO:0000345
has_obo_namespace: eco
created by: mchibucos

Support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: transcript expression evidence used in manual assertion
subclass of: Support of intron position by RNA-seq alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2013-04-04T04:37:35Z
definition: A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
id: ECO:0000346
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: transcript expression evidence used in manual assertion
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Support of intron position by RNA-seq alignment evidence
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2013-04-04T04:38:45Z
definition: A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
id: ECO:0000347
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated target: evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Full support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Full support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Full support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2013-04-04T04:40:19Z
definition: A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
id: ECO:0000348
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
annotated target: A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: Full support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition

Full support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Full support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Full support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2013-04-04T04:41:34Z
definition: A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
id: ECO:0000349
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Full support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition

Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: Partial support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: Partial support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2013-04-04T04:42:53Z
definition: A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
id: ECO:0000350
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: Support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: Partial support of intron position by RNA-seq alignment evidence used in automatic assertion
annotated property: definition

Partial support of intron position by RNA-seq alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Partial support of intron position by RNA-seq alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: Partial support of intron position by RNA-seq alignment evidence
subclass of: Support of intron position by RNA-seq alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2013-04-04T04:43:56Z
definition: A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
id: ECO:0000351
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: Support of intron position by RNA-seq alignment evidence used in manual assertion
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Partial support of intron position by RNA-seq alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
definition: A type of evidence that is used in an manual assertion.
id: ECO:0000352
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: evidence
annotated source: evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of evidence that is used in an manual assertion.
database_cross_reference: ECO:MCC
annotated source: evidence used in manual assertion
annotated property: definition

physical interaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: physical interaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2013-10-31T21:01:27Z
definition: A type of physical interaction evidence that is used in a manual assertion.
id: ECO:0000353
database_cross_reference: GOECO:IPI
has_exact_synonym: IPI
has_obo_namespace: eco
has_exact_synonym: inferred from physical interaction
created by: mchibucos
in_subset: valid with chemical entity
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
database_cross_reference: GOECO:IPI
annotated target: inferred from physical interaction
annotated source: physical interaction evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
database_cross_reference: GOECO:IPI
annotated target: IPI
annotated source: physical interaction evidence used in manual assertion
annotated property: has_exact_synonym

Axiom
annotated target: A type of physical interaction evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: physical interaction evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: physical interaction evidence used in manual assertion
annotated property: subClassOf

inferred from physical interaction
type: Axiom
annotated target: GOECO:IPI
annotated source: physical interaction evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: physical interaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

gene neighbors evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: gene neighbors evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: gene neighbors evidence
subclass of: genomic context evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2013-11-19T13:37:24Z
definition: A type of gene neighbors evidence that is used in a manual assertion.
id: ECO:0000354
database_cross_reference: GO_REF:0000025
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of gene neighbors evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: gene neighbors evidence used in manual assertion
annotated property: definition

operon structure as IGC evidence
type: Axiom
annotated target: GO_REF:0000025
annotated source: gene neighbors evidence used in manual assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated source: gene neighbors evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

phylogenetic distribution evidence
type: Class
subclass of: phylogenetic evidence
creation date: 2014-04-22T17:02:31Z
definition: A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony.
id: ECO:0000355
comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony.
database_cross_reference: ECO:MCC
database_cross_reference: PMID:21238344
annotated source: phylogenetic distribution evidence
annotated property: definition

differential gene expression evidence from microarray experiment
type: Class
subclass of: transcript expression evidence
creation date: 2014-09-26T16:21:45Z
id: ECO:0000356
has_obo_namespace: eco
created by: mchibucos

differential gene expression evidence from RNA-seq experiment
type: Class
subclass of: transcript expression evidence
creation date: 2014-09-26T16:22:57Z
id: ECO:0000357
has_obo_namespace: eco
created by: mchibucos

differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact)
type: Class
subclass of: transcript expression evidence
creation date: 2014-09-26T16:25:47Z
id: ECO:0000358
has_obo_namespace: eco
created by: mchibucos

differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact)
type: Class
subclass of: transcript expression evidence
creation date: 2014-09-26T16:26:48Z
id: ECO:0000359
has_obo_namespace: eco
created by: mchibucos

biological target-disease association via drug
type: Class
subclass of: experimental evidence
creation date: 2014-09-26T16:39:11Z
id: ECO:0000360
has_obo_namespace: eco
created by: mchibucos

inferential evidence
type: Class
subclass of: evidence
creation date: 2015-07-14T11:19:37Z
definition: A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means.
id: ECO:0000361
comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true.
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means.
database_cross_reference: ECO:MCC
annotated source: inferential evidence
annotated property: definition

evidence based on computational logical inference
type: Class
subclass of: inferential evidence
creation date: 2015-07-14T11:24:27Z
definition: A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is computationally derived.
id: ECO:0000362
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is computationally derived.
database_cross_reference: ECO:MCC
database_cross_reference: GOC:MC
annotated source: evidence based on computational logical inference
annotated property: definition

evidence based on computational logical inference used in automatic assertion
type: Class
equivalent class: Class
  intersection of: evidence based on computational logical inference
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence based on computational logical inference
subclass of: evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2015-07-14T11:35:08Z
definition: A type of evidence based on computational logical inference that is used in automatic assertion.
id: ECO:0000363
database_cross_reference: GO_REF:0000108
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: evidence based on computational logical inference used in automatic assertion
annotated target: evidence used in automatic assertion
annotated property: subClassOf

Automatic assignment of GO terms using logical inference, based on on inter-ontology links
type: Axiom
annotated target: GO_REF:0000108
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: database_cross_reference

Axiom
annotated target: A type of evidence based on computational logical inference that is used in automatic assertion.
database_cross_reference: ECO:MCC
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: evidence based on computational logical inference used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

evidence based on logical inference from manual annotation used in automatic assertion
type: Class
subclass of: evidence based on computational logical inference used in automatic assertion
creation date: 2015-07-14T11:44:19Z
definition: A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion.
id: ECO:0000364
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: evidence based on logical inference from manual annotation used in automatic assertion
annotated property: definition

evidence based on logical inference from automatic annotation used in automatic assertion
type: Class
subclass of: evidence based on computational logical inference used in automatic assertion
creation date: 2015-07-14T11:45:25Z
definition: A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion.
id: ECO:0000366
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion.
database_cross_reference: ECO:MCC
annotated source: evidence based on logical inference from automatic annotation used in automatic assertion
annotated property: definition

evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
definition: A type of evidence that is used in an automatic assertion.
id: ECO:0000501
database_cross_reference: GOECO:IEA
database_cross_reference: GO_REF:0000004
database_cross_reference: GO_REF:0000023
has_exact_synonym: IEA
created by: cjm
has_obo_namespace: eco
has_exact_synonym: inferred from electronic annotation
in_subset: valid with gene
in_subset: valid with protein
in_subset: valid with protein complex

Axiom
database_cross_reference: GOECO:IEA
annotated target: IEA
annotated source: evidence used in automatic assertion
annotated property: has_exact_synonym

Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.
type: Axiom
annotated target: GO_REF:0000023
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference

Axiom
is inferred: true
annotated target: evidence
annotated source: evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of evidence that is used in an automatic assertion.
database_cross_reference: ECO:cjm
annotated source: evidence used in automatic assertion
annotated property: definition

inferred from electronic annotation
type: Axiom
annotated target: GOECO:IEA
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference

Axiom
database_cross_reference: GOECO:IEA
annotated target: inferred from electronic annotation
annotated source: evidence used in automatic assertion
annotated property: has_exact_synonym

Axiom
is inferred: true
annotated source: evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Gene Ontology annotation based on Swiss-Prot keyword mapping.
type: Axiom
annotated target: GO_REF:0000004
annotated source: evidence used in automatic assertion
annotated property: database_cross_reference

3D cell culture evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001001
has_obo_namespace: eco
created by: mchibucos

[3H]arachidonic acid release assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001002
has_obo_namespace: eco
created by: mchibucos

[3H]-thymidine incorporation assay evidence
type: Class
subclass of: DNA synthesis cell proliferation assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001003
has_obo_namespace: eco
created by: mchibucos

51Cr release assay evidence
type: Class
subclass of: cytotoxicity assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001004
has_obo_namespace: eco
created by: mchibucos

7-aminoactinomycin staining evidence
type: Class
subclass of: staining evidence
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: 7-AAD staining evidence
has_exact_synonym: 7-amino-actinomycin D staining evidence
id: ECO:0001005
has_obo_namespace: eco
created by: mchibucos

adhesion assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001006
has_obo_namespace: eco
created by: mchibucos

adoptive cell transfer evidence
type: Class
subclass of: ex vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.
has_exact_synonym: Adoptive immunotherapy
id: ECO:0001007
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: passive immunization

Axiom
annotated target: A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.
database_cross_reference: ECO:SN
database_cross_reference: url:http://jco.ascopubs.org/content/23/10/2346.full.pdf+html
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf
annotated source: adoptive cell transfer evidence
annotated property: definition

Alamar blue assay evidence
type: Class
subclass of: cell viability assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001008
has_obo_namespace: eco
created by: mchibucos

allograft transplantation experiment evidence
type: Class
subclass of: anatomical perturbation evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype.
has_exact_synonym: Allotransplantation
id: ECO:0001009
has_exact_synonym: allografting
has_obo_namespace: eco
created by: mchibucos
has_narrow_synonym: tissue grafting

Axiom
annotated target: A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/subjects/allograft
database_cross_reference: url:http://www.organdonor.gov/about/terms_and_topics/
annotated source: allograft transplantation experiment evidence
annotated property: definition

anion-exchange chromatography evidence
type: Class
subclass of: chromatography evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules.
id: ECO:0001010
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules.
database_cross_reference: ECO:SN
database_cross_reference: PMID:20978968
database_cross_reference: url:http://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography
annotated source: anion-exchange chromatography evidence
annotated property: definition

annexin-V staining evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001011
has_obo_namespace: eco
created by: mchibucos

behavioral assay evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001012
has_obo_namespace: eco
created by: mchibucos

blocking monoclonal antibody evidence
type: Class
subclass of: immunological assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001013
has_obo_namespace: eco
created by: mchibucos

blocking peptide evidence
type: Class
subclass of: immunological assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001014
has_obo_namespace: eco
created by: mchibucos

blocking polyclonal antibody evidence
type: Class
subclass of: immunological assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001015
has_obo_namespace: eco
created by: mchibucos

blood test evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components.
id: ECO:0001016
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components.
database_cross_reference: ECO:SN
database_cross_reference: url:https://www.nhlbi.nih.gov/health/health-topics/topics/bdt
annotated source: blood test evidence
annotated property: definition

Boyden chamber assay evidence
type: Class
subclass of: chemotaxis assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001017
has_obo_namespace: eco
created by: mchibucos

bromodeoxyuridine incorporation assay evidence
type: Class
subclass of: nucleotide analog incorporation evidence
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: 5-bromo-2'-deoxyuridine incorporation assay evidence
has_exact_synonym: BUdR incorporation assay evidence
has_exact_synonym: BrdU incorporation assay evidence
has_exact_synonym: BrdUrd incorporation assay evidence
id: ECO:0001018
has_obo_namespace: eco
created by: mchibucos

caspase assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001019
has_obo_namespace: eco
created by: mchibucos

cell counting evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001020
has_obo_namespace: eco
created by: mchibucos

cell permeability assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001021
has_obo_namespace: eco
created by: mchibucos

carboxyfluorescein diacetate succinimidyl ester staining evidence
type: Class
subclass of: staining evidence
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: CFDA-SE staining evidence
has_exact_synonym: CFSE-staining evidence
id: ECO:0001022
has_obo_namespace: eco
created by: mchibucos

chemiluminescence-linked immunoassay evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: CLIA evidence
id: ECO:0001023
has_obo_namespace: eco
created by: mchibucos

chimeric protein evidence
type: Class
subclass of: mutant phenotype evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001024
has_obo_namespace: eco
created by: mchibucos

co-electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001025
has_obo_namespace: eco
created by: mchibucos

co-localization evidence
type: Class
subclass of: imaging assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001026
has_obo_namespace: eco
created by: mchibucos

colony counting evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001027
has_obo_namespace: eco
created by: mchibucos

co-sedimentation assay evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001028
has_obo_namespace: eco
created by: mchibucos

comet assay evidence
type: Class
subclass of: gel electrophoresis evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001029
has_obo_namespace: eco
created by: mchibucos

conditional knockout evidence
type: Class
subclass of: knockout evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001030
has_obo_namespace: eco
created by: mchibucos

conditional knockin evidence
type: Class
subclass of: knockin evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001031
has_obo_namespace: eco
created by: mchibucos

constitutively active mutant evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001032
has_obo_namespace: eco
created by: mchibucos

cross-linking evidence
type: Class
subclass of: protein binding evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001033
has_obo_namespace: eco
created by: mchibucos

crystallography evidence
type: Class
subclass of: structure determination evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001034
has_obo_namespace: eco
created by: mchibucos

cytochemistry evidence
type: Class
subclass of: expression pattern evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001035
has_obo_namespace: eco
created by: mchibucos

cytochrome C release assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001036
has_obo_namespace: eco
created by: mchibucos

4',6-diamidino-2-phenylindole staining evidence
type: Class
subclass of: staining evidence
creation date: 2014-03-16T11:30:54Z
has_exact_synonym: DAPI staining evidence
id: ECO:0001037
has_obo_namespace: eco
created by: mchibucos

deletion evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001038
has_obo_namespace: eco
created by: mchibucos

DNA laddering assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001039
has_obo_namespace: eco
created by: mchibucos

RNA dot blot assay evidence
type: Class
subclass of: RNA detection assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments.
id: ECO:0001040
has_related_synonym: RNA dot blot
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21424648
annotated source: RNA dot blot assay evidence
annotated property: definition

RNA microarray evidence
type: Class
subclass of: RNA detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001041
has_obo_namespace: eco
created by: mchibucos

dominant-negative mutant evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001042
has_obo_namespace: eco
created by: mchibucos

Edman degradation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Edman degradation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: Edman degradation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:59:00Z
definition: A type of Edman degradation evidence that is used in a manual assertion.
id: ECO:0001043
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of Edman degradation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Edman degradation evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: Edman degradation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: Edman degradation evidence used in manual assertion
annotated property: subClassOf

Edman degradation evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001044
has_obo_namespace: eco
created by: mchibucos

eTag assay evidence
type: Class
subclass of: affinity evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001045
has_obo_namespace: eco
created by: mchibucos

filter binding assay evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001046
has_obo_namespace: eco
created by: mchibucos

fluorescence in situ hybridization evidence
type: Class
subclass of: expression pattern evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001047
has_obo_namespace: eco
created by: mchibucos

fluorescence resonance energy transfer evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001048
has_exact_synonym: FRET
has_obo_namespace: eco
created by: mchibucos

gel-filtration evidence
type: Class
subclass of: chromatography evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001049
has_obo_namespace: eco
created by: mchibucos

histochemistry evidence
type: Class
subclass of: expression pattern evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001050
has_obo_namespace: eco
created by: mchibucos

histology evidence
type: Class
subclass of: imaging assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001051
has_obo_namespace: eco
created by: mchibucos

high-performance liquid chromatography evidence
type: Class
subclass of: chromatography evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001052
has_exact_synonym: HPLC evidence
has_obo_namespace: eco
created by: mchibucos

immunocytochemistry evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001053
has_obo_namespace: eco
created by: mchibucos

immunodepletion evidence
type: Class
subclass of: immunological assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001054
has_obo_namespace: eco
created by: mchibucos

immunohistochemistry evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001055
has_obo_namespace: eco
created by: mchibucos

induced mutation evidence
type: Class
subclass of: genetic transformation evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001056
has_obo_namespace: eco
created by: mchibucos

in vitro acetylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
id: ECO:0001057
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml
annotated source: in vitro acetylation assay evidence
annotated property: definition

in vitro cleavage assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments.
id: ECO:0001058
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments.
database_cross_reference: ECO:SN
database_cross_reference: PMID:21121091
database_cross_reference: SIB:PG
annotated source: in vitro cleavage assay evidence
annotated property: definition

in vitro deubiquitination assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro.
id: ECO:0001059
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG
annotated source: in vitro deubiquitination assay evidence
annotated property: definition

in vitro deacetylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment.
id: ECO:0001060
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml
annotated source: in vitro deacetylation assay evidence
annotated property: definition

in vitro defarnesylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment.
id: ECO:0001061
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:16126733
database_cross_reference: SIB:PG
annotated source: in vitro defarnesylation assay evidence
annotated property: definition

in vitro demethylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment.
id: ECO:0001062
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Demethylation
annotated source: in vitro demethylation assay evidence
annotated property: definition

in vitro desumoylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro.
id: ECO:0001063
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/
annotated source: in vitro desumoylation assay evidence
annotated property: definition

in vitro farnesylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro experiment.
id: ECO:0001064
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Farnesyltransferase
annotated source: in vitro farnesylation assay evidence
annotated property: definition

in vitro methylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
id: ECO:0001065
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Methylation
annotated source: in vitro methylation assay evidence
annotated property: definition

in vitro palmitoylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment.
id: ECO:0001066
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17077383
database_cross_reference: SIB:PG
annotated source: in vitro palmitoylation assay evidence
annotated property: definition

in vitro phosphatase assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment.
id: ECO:0001067
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Phosphatase
annotated source: in vitro phosphatase assay evidence
annotated property: definition

in vitro protein kinase assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment.
id: ECO:0001068
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_kinase
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_phosphorylation
annotated source: in vitro protein kinase assay evidence
annotated property: definition

in vitro polyADP-ribosylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment.
id: ECO:0001069
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:21870253
database_cross_reference: SIB:PG
annotated source: in vitro polyADP-ribosylation assay evidence
annotated property: definition

in vitro sumoylation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment.
id: ECO:0001070
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/
annotated source: in vitro sumoylation assay evidence
annotated property: definition

in vitro transcription assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions.
id: ECO:0001071
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW
database_cross_reference: PMID:21125481
database_cross_reference: SIB:PG
annotated source: in vitro transcription assay evidence
annotated property: definition

in vitro translation assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment.
id: ECO:0001072
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:18230759
database_cross_reference: SIB:PG
annotated source: in vitro translation assay evidence
annotated property: definition

in vitro ubiquitination assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment.
id: ECO:0001073
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG
annotated source: in vitro ubiquitination assay evidence
annotated property: definition

in vivo acetylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo.
id: ECO:0001074
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml
annotated source: in vivo acetylation assay evidence
annotated property: definition

in vivo cleavage assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments.
id: ECO:0001075
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22154596
database_cross_reference: SIB:PG
annotated source: in vivo cleavage assay evidence
annotated property: definition

in vivo deacetylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo.
id: ECO:0001076
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml
annotated source: in vivo deacetylation assay evidence
annotated property: definition

in vivo defarnesylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo.
id: ECO:0001077
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15556768
database_cross_reference: PMID:16507103
database_cross_reference: SIB:PG
annotated source: in vivo defarnesylation assay evidence
annotated property: definition

in vivo demethylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo.
id: ECO:0001078
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Demethylation
annotated source: in vivo demethylation assay evidence
annotated property: definition

in vivo deubiquitination assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo.
id: ECO:0001079
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG
annotated source: in vivo deubiquitination assay evidence
annotated property: definition

in vivo desumoylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo.
id: ECO:0001080
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/
annotated source: in vivo desumoylation assay evidence
annotated property: definition

in vivo farnesylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo.
id: ECO:0001081
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:9030603
database_cross_reference: SIB:PG
annotated source: in vivo farnesylation assay evidence
annotated property: definition

in vivo methylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo.
id: ECO:0001082
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Methylation
annotated source: in vivo methylation assay evidence
annotated property: definition

in vivo palmitoylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo.
id: ECO:0001083
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:10329400
database_cross_reference: SIB:PG
annotated source: in vivo palmitoylation assay evidence
annotated property: definition

in vivo phosphatase assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo.
id: ECO:0001084
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Phosphatase
annotated source: in vivo phosphatase assay evidence
annotated property: definition

in vivo protein kinase assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo.
id: ECO:0001085
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_kinase
database_cross_reference: url:http://en.wikipedia.org/wiki/Protein_phosphorylation
annotated source: in vivo protein kinase assay evidence
annotated property: definition

in vivo sumoylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo.
id: ECO:0001086
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: SIB:PG
database_cross_reference: url:http://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/
annotated source: in vivo sumoylation assay evidence
annotated property: definition

in vivo transcription assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where transcription is detected in cells in vivo.
id: ECO:0001087
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where transcription is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:12181418
database_cross_reference: SIB:PG
annotated source: in vivo transcription assay evidence
annotated property: definition

in vivo translation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo.
id: ECO:0001088
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:24901308
database_cross_reference: SIB:PG
annotated source: in vivo translation assay evidence
annotated property: definition

in vivo ubiquitination assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo.
id: ECO:0001089
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19692941
database_cross_reference: SIB:PG
annotated source: in vivo ubiquitination assay evidence
annotated property: definition

knockin evidence
type: Class
subclass of: genetic transformation evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001090
has_obo_namespace: eco
created by: mchibucos

knockout evidence
type: Class
subclass of: genetic transformation evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001091
has_obo_namespace: eco
created by: mchibucos

lipid binding assay evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001092
has_obo_namespace: eco
created by: mchibucos

luminescence-based mammalian interactome mapping assay evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001093
has_exact_synonym: LUMIER assay
has_obo_namespace: eco
created by: mchibucos

macroscopy evidence
type: Class
subclass of: experimental evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001094
has_obo_namespace: eco
created by: mchibucos

mammalian 2-hybrid assay evidence
type: Class
subclass of: hybrid interaction evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001095
has_obo_namespace: eco
created by: mchibucos

mass spectrometry evidence
type: Class
subclass of: spectrometry evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001096
has_obo_namespace: eco
created by: mchibucos

medical imaging evidence
type: Class
subclass of: imaging assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001097
has_obo_namespace: eco
created by: mchibucos

microscopy evidence
type: Class
subclass of: imaging assay evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of direct assay evidence where a microscope was used to view a sample.
id: ECO:0001098
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of direct assay evidence where a microscope was used to view a sample.
database_cross_reference: ECO:MCC
annotated source: microscopy evidence
annotated property: definition

motility wound healing assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001099
has_obo_namespace: eco
created by: mchibucos

MTS assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
id: ECO:0001100
has_obo_namespace: eco
created by: mchibucos

MTT assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
has_narrow_synonym: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
id: ECO:0001101
has_obo_namespace: eco
created by: mchibucos

multiplex bead-based immunoassay evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001102
has_obo_namespace: eco
created by: mchibucos

natural variation mutant evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001103
has_obo_namespace: eco
created by: mchibucos

nuclear fragmentation evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001104
has_obo_namespace: eco
created by: mchibucos

nuclear magnetic resonance spectroscopy evidence
type: Class
subclass of: structure determination evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001105
has_exact_synonym: NMR spectroscopy
has_obo_namespace: eco
created by: mchibucos

nuclease protection assay evidence
type: Class
subclass of: RNA detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001106
has_obo_namespace: eco
created by: mchibucos

nucleotide analog incorporation evidence
type: Class
subclass of: DNA synthesis cell proliferation assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001107
has_obo_namespace: eco
created by: mchibucos

phage display evidence
type: Class
subclass of: protein binding evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001108
has_obo_namespace: eco
created by: mchibucos

phosphoamino acid analysis evidence
type: Class
subclass of: protein assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001109
has_obo_namespace: eco
created by: mchibucos

peptide affinity enrichment evidence
type: Class
subclass of: affinity evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001110
has_obo_namespace: eco
created by: mchibucos
has_narrow_synonym: phosphopeptide affinity enrichment evidence

physical examination evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001111
has_obo_namespace: eco
created by: mchibucos

peptide array evidence
type: Class
subclass of: protein assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001112
has_obo_namespace: eco
created by: mchibucos
has_narrow_synonym: phosphopeptide array evidence

point mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001113
has_obo_namespace: eco
created by: mchibucos

propidium iodide staining evidence
type: Class
subclass of: staining evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001114
has_obo_namespace: eco
created by: mchibucos

fluorescence evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001115
has_obo_namespace: eco
created by: mchibucos

protein dot blot assay evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001116
has_obo_namespace: eco
created by: mchibucos

protein microarray evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001117
has_obo_namespace: eco
created by: mchibucos

protein sequencing assay evidence
type: Class
subclass of: sequencing assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001118
has_obo_namespace: eco
created by: mchibucos

quantitative mass spectrometry evidence
type: Class
subclass of: mass spectrometry evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001119
has_obo_namespace: eco
created by: mchibucos

radioisotope assay evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001120
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: radioassay
has_exact_synonym: radioisotopic assay

radioimmunoassay evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001121
has_obo_namespace: eco
created by: mchibucos

resonant mirror biosensor evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001123
has_obo_namespace: eco
created by: mchibucos

restriction fragment detection evidence
type: Class
subclass of: DNA detection assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001124
has_obo_namespace: eco
created by: mchibucos

spectrophotometry evidence
type: Class
subclass of: spectrometry evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001126
has_obo_namespace: eco
created by: mchibucos

surface plasmon resonance evidence
type: Class
subclass of: physical interaction evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction.
id: ECO:0001127
comment: Plasmons are generated by an incident light beam.
has_exact_synonym: SPR evidence
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11578932
database_cross_reference: PMID:19151937
database_cross_reference: PMID:8574707
annotated source: surface plasmon resonance evidence
annotated property: definition

Axiom
database_cross_reference: PMID:11578932
annotated target: SPR evidence
annotated source: surface plasmon resonance evidence
annotated property: has_exact_synonym

syngeneic transplantation experiment evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001128
has_obo_namespace: eco
created by: mchibucos

TACE activity assay evidence
type: Class
subclass of: enzyme assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001129
has_exact_synonym: Tumor necrosis factor - alpha converting enzyme activity assay
has_obo_namespace: eco
created by: mchibucos

tissue microarray evidence
type: Class
subclass of: array experiment evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001130
has_obo_namespace: eco
created by: mchibucos

transgenic organism evidence
type: Class
subclass of: genetic transformation evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001131
has_obo_namespace: eco
created by: mchibucos

tryptic phosphopeptide mapping assay evidence
type: Class
subclass of: mass spectrometry evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001132
has_obo_namespace: eco
created by: mchibucos

terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
type: Class
subclass of: apoptotic assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001133
has_exact_synonym: TUNEL assay evidence
has_exact_synonym: TdT-mediated dUTP-biotin nick end labeling assay
has_obo_namespace: eco
created by: mchibucos
has_exact_synonym: terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence

urine test evidence
type: Class
subclass of: direct assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001134
has_obo_namespace: eco
created by: mchibucos

wound healing assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001135
has_obo_namespace: eco
created by: mchibucos

WST-1 assay evidence
type: Class
subclass of: cell proliferation assay evidence
creation date: 2014-03-16T11:30:54Z
id: ECO:0001136
has_obo_namespace: eco
created by: mchibucos

xenotransplantation experiment evidence
type: Class
subclass of: anatomical perturbation evidence
creation date: 2014-03-16T11:30:54Z
definition: A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient.
id: ECO:0001137
has_exact_synonym: Xenograft transplantation
has_exact_synonym: Xenografting
has_exact_synonym: Xenotransplantation
has_obo_namespace: eco
created by: mchibucos
has_narrow_synonym: tissue grafting

Axiom
annotated target: A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient.
database_cross_reference: ECO:SN
database_cross_reference: url:http://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html
database_cross_reference: url:http://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/
annotated source: xenotransplantation experiment evidence
annotated property: definition

3D cell culture evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 3D cell culture evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 3D cell culture evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:18:35Z
definition: A type of 3D cell culture evidence that is used in a manual assertion.
id: ECO:0001138
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: 3D cell culture evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: 3D cell culture evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of 3D cell culture evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: 3D cell culture evidence used in manual assertion
annotated property: definition

51Cr release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 51Cr release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 51Cr release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:20:55Z
definition: A type of 51Cr release assay evidence that is used in a manual assertion.
id: ECO:0001139
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of 51Cr release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: 51Cr release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

7-aminoactinomycin staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 7-aminoactinomycin staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 7-aminoactinomycin staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:22:01Z
definition: A type of 7-aminoactinomycin staining evidence that is used in a manual assertion.
id: ECO:0001140
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of 7-aminoactinomycin staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: 7-aminoactinomycin staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

[3H]-thymidine incorporation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: [3H]-thymidine incorporation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: [3H]-thymidine incorporation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:31:52Z
definition: A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion.
id: ECO:0001141
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: [3H]-thymidine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

[3H]arachidonic acid release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: [3H]arachidonic acid release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: [3H]arachidonic acid release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:32:44Z
definition: A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion.
id: ECO:0001142
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: [3H]arachidonic acid release assay evidence used in manual assertion
annotated property: definition

adhesion assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: adhesion assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: adhesion assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:33:38Z
definition: A type of adhesion assay evidence that is used in a manual assertion.
id: ECO:0001143
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: adhesion assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of adhesion assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: adhesion assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: adhesion assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

adoptive cell transfer evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: adoptive cell transfer evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: adoptive cell transfer evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:36:19Z
definition: A type of adoptive cell transfer evidence that is used in a manual assertion.
id: ECO:0001144
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of adoptive cell transfer evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: adoptive cell transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Alamar blue assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Alamar blue assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Alamar blue assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:37:21Z
definition: A type of Alamar blue assay evidence that is used in a manual assertion.
id: ECO:0001145
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of Alamar blue assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Alamar blue assay evidence used in manual assertion
annotated property: definition

allograft transplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: allograft transplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: allograft transplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:38:22Z
definition: A type of allograft transplantation experiment evidence that is used in a manual assertion.
id: ECO:0001146
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of allograft transplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: allograft transplantation experiment evidence used in manual assertion
annotated property: subClassOf

anion-exchange chromatography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: anion-exchange chromatography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: anion-exchange chromatography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:44:30Z
definition: A type of anion-exchange chromatography evidence that is used in a manual assertion.
id: ECO:0001147
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of anion-exchange chromatography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: anion-exchange chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

annexin-V staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: annexin-V staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: annexin-V staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:52:01Z
definition: A type of annexin-V staining evidence that is used in a manual assertion.
id: ECO:0001148
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: annexin-V staining evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of annexin-V staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: annexin-V staining evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: annexin-V staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

behavioral assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: behavioral assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: behavioral assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:53:38Z
definition: A type of behavioral assay evidence used that is used in a manual assertion.
id: ECO:0001149
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: behavioral assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of behavioral assay evidence used that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: behavioral assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: behavioral assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

blocking monoclonal antibody evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking monoclonal antibody evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking monoclonal antibody evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:54:44Z
definition: A type of blocking monoclonal antibody evidence that is used in a manual assertion.
id: ECO:0001150
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of blocking monoclonal antibody evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: blocking monoclonal antibody evidence used in manual assertion
annotated property: subClassOf

blocking peptide evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking peptide evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking peptide evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T15:55:33Z
definition: A type of blocking peptide evidence that is used in a manual assertion.
id: ECO:0001151
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: blocking peptide evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of blocking peptide evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: blocking peptide evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: blocking peptide evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

blocking polyclonal antibody evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blocking polyclonal antibody evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: blocking polyclonal antibody evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:01:39Z
definition: A type of blocking polyclonal antibody evidence tthat is used in a manual assertion.
id: ECO:0001152
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of blocking polyclonal antibody evidence tthat is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: blocking polyclonal antibody evidence used in manual assertion
annotated property: subClassOf

blood test evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: blood test evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: blood test evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:02:47Z
definition: A type of blood test evidence that is used in a manual assertion.
id: ECO:0001153
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of blood test evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: blood test evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: blood test evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: blood test evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Boyden chamber assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: Boyden chamber assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: Boyden chamber assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:03:53Z
definition: A type of Boyden chamber assay evidence that is used in a manual assertion.
id: ECO:0001154
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of Boyden chamber assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: Boyden chamber assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

bromodeoxyuridine incorporation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: bromodeoxyuridine incorporation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: bromodeoxyuridine incorporation assay evidence
subclass of: nucleotide analog incorporation evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:04:49Z
definition: A type of BrdU incorporation assay evidence that is used in a manual assertion.
id: ECO:0001155
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of BrdU incorporation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: bromodeoxyuridine incorporation assay evidence used in manual assertion
annotated target: nucleotide analog incorporation evidence used in manual assertion
annotated property: subClassOf

caspase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: caspase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: caspase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:06:06Z
definition: A type of caspase assay evidence that is used in a manual assertion.
id: ECO:0001156
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: caspase assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of caspase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: caspase assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: caspase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

cell counting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell counting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cell counting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:07:13Z
definition: A type of cell counting evidence that is used in a manual assertion.
id: ECO:0001157
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: cell counting evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: cell counting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of cell counting evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cell counting evidence used in manual assertion
annotated property: definition

cell permeability assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cell permeability assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cell permeability assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:07:58Z
definition: A type of cell permeability assay evidence that is used in a manual assertion.
id: ECO:0001158
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: cell permeability assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of cell permeability assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cell permeability assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: cell permeability assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: carboxyfluorescein diacetate succinimidyl ester staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: carboxyfluorescein diacetate succinimidyl ester staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:08:54Z
definition: A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion.
id: ECO:0001159
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

chemiluminescence-linked immunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chemiluminescence-linked immunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: chemiluminescence-linked immunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:09:55Z
definition: A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion.
id: ECO:0001160
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: chemiluminescence-linked immunoassay evidence used in manual assertion
annotated property: subClassOf

chimeric protein evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: chimeric protein evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: mutant phenotype evidence used in manual assertion
subclass of: chimeric protein evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:10:40Z
definition: A type of chimeric protein evidence that is used in a manual assertion.
id: ECO:0001161
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of chimeric protein evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: chimeric protein evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: chimeric protein evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: mutant phenotype evidence used in manual assertion
annotated source: chimeric protein evidence used in manual assertion
annotated property: subClassOf

co-electrophoresis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-electrophoresis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: co-electrophoresis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:11:22Z
definition: A type of co-electrophoresis evidence that is used in a manual assertion.
id: ECO:0001162
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of co-electrophoresis evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: co-electrophoresis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

co-localization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-localization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: co-localization evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:12:04Z
definition: A type of co-localization evidence that is used in a manual assertion.
id: ECO:0001163
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: co-localization evidence used in manual assertion
annotated target: imaging assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: co-localization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of co-localization evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: co-localization evidence used in manual assertion
annotated property: definition

co-sedimentation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: co-sedimentation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: co-sedimentation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:12:55Z
definition: A type of co-sedimentation assay evidence that is used in a manual assertion.
id: ECO:0001164
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of co-sedimentation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: co-sedimentation assay evidence used in manual assertion
annotated property: subClassOf

colony counting evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: colony counting evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: colony counting evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:14:57Z
definition: A type of colony counting evidence that is used in a manual assertion.
id: ECO:0001165
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of colony counting evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: colony counting evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: colony counting evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: colony counting evidence used in manual assertion
annotated property: subClassOf

comet assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: comet assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: comet assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:16:01Z
definition: A type of comet assay evidence that is used in a manual assertion.
id: ECO:0001166
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of comet assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: comet assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: comet assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: comet assay evidence used in manual assertion
annotated property: subClassOf

conditional knockin evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: conditional knockin evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: conditional knockin evidence
subclass of: knockin evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:17:22Z
definition: A type of conditional knockin evidence that is used in a manual assertion.
id: ECO:0001167
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of conditional knockin evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: conditional knockin evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: conditional knockin evidence used in manual assertion
annotated target: knockin evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: conditional knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

conditional knockout evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: conditional knockout evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: conditional knockout evidence
subclass of: knockout evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:17:58Z
definition: A type of conditional knockout evidence that is used in a manual assertion.
id: ECO:0001168
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of conditional knockout evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: conditional knockout evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: conditional knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: conditional knockout evidence used in manual assertion
annotated target: knockout evidence used in manual assertion
annotated property: subClassOf

constitutively active mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: constitutively active mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: constitutively active mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:20:23Z
definition: A type of constitutively active mutant evidence that is used a in manual assertion.
id: ECO:0001169
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of constitutively active mutant evidence that is used a in manual assertion.
database_cross_reference: ECO:MCC
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: constitutively active mutant evidence used in manual assertion
annotated property: subClassOf

cross-linking evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cross-linking evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: cross-linking evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:21:29Z
definition: A type of cross-linking evidence that is used in a manual assertion.
id: ECO:0001170
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of cross-linking evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cross-linking evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: cross-linking evidence used in manual assertion
annotated property: subClassOf

crystallography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: crystallography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: crystallography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:22:20Z
definition: A type of crystallography evidence that is used in a manual assertion.
id: ECO:0001171
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: crystallography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of crystallography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: crystallography evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: crystallography evidence used in manual assertion
annotated property: subClassOf

cytochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cytochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: cytochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:23:10Z
definition: A type of cytochemistry evidence that is used in a manual assertion.
id: ECO:0001172
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: cytochemistry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of cytochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cytochemistry evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: cytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

cytochrome C release assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: cytochrome C release assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: cytochrome C release assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:23:53Z
definition: A type of cytochrome C release assay evidence that is used in a manual assertion.
id: ECO:0001173
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of cytochrome C release assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: cytochrome C release assay evidence used in manual assertion
annotated property: subClassOf

4',6-diamidino-2-phenylindole staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: 4',6-diamidino-2-phenylindole staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: 4',6-diamidino-2-phenylindole staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:25:03Z
definition: A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion.
id: ECO:0001174
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion
annotated property: subClassOf

deletion evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: deletion evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: deletion evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:25:54Z
definition: A type of deletion evidence that is used in a manual assertion.
id: ECO:0001175
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: deletion evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: deletion evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of deletion evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: deletion evidence used in manual assertion
annotated property: definition

DNA laddering assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA laddering assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: DNA laddering assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:26:46Z
definition: A type of DNA laddering assay evidence that is used in a manual assertion.
id: ECO:0001176
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of DNA laddering assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: DNA laddering assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

RNA dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: RNA dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:45:52Z
definition: A type of RNA dot blot assay evidence that is used in a manual assertion.
id: ECO:0001177
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of RNA dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: RNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

RNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: RNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: RNA microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:46:50Z
definition: A type of RNA microarray evidence that is used in a manual assertion.
id: ECO:0001178
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: RNA microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: RNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of RNA microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: RNA microarray evidence used in manual assertion
annotated property: definition

dominant-negative mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: dominant-negative mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: dominant-negative mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:47:44Z
definition: A type of dominant-negative mutant evidence that is used in a manual assertion.
id: ECO:0001179
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of dominant-negative mutant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: dominant-negative mutant evidence used in manual assertion
annotated property: subClassOf

eTag assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: eTag assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: eTag assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T16:59:39Z
definition: A type of eTag assay evidence that is used in a manual assertion.
id: ECO:0001180
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: eTag assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: eTag assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of eTag assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: eTag assay evidence used in manual assertion
annotated property: definition

filter binding assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: filter binding assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: filter binding assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:03:28Z
definition: A type of filter binding assay evidence that is used in a manual assertion.
id: ECO:0001181
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: filter binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: filter binding assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of filter binding assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: filter binding assay evidence used in manual assertion
annotated property: definition

fluorescence in situ hybridization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence in situ hybridization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: fluorescence in situ hybridization evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:04:20Z
definition: A type of fluorescence in situ hybridization evidence that is used in a manual assertion.
id: ECO:0001182
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of fluorescence in situ hybridization evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: fluorescence in situ hybridization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

fluorescence resonance energy transfer evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence resonance energy transfer evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: fluorescence resonance energy transfer evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:05:22Z
definition: A type of fluorescence resonance energy transfer evidence that is used in a manual assertion.
id: ECO:0001183
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of fluorescence resonance energy transfer evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: fluorescence resonance energy transfer evidence used in manual assertion
annotated property: definition

gel-filtration evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: gel-filtration evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: gel-filtration evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:06:14Z
definition: A type of gel-filtration evidence that is used in a manual assertion.
id: ECO:0001184
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: gel-filtration evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of gel-filtration evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: gel-filtration evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: gel-filtration evidence used in manual assertion
annotated property: subClassOf

histochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: histochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: expression evidence used in manual assertion
subclass of: histochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:07:19Z
definition: A type of histochemistry evidence that is used in a manual assertion.
id: ECO:0001185
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: expression evidence used in manual assertion
annotated source: histochemistry evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: histochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of histochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: histochemistry evidence used in manual assertion
annotated property: definition

immunocytochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunocytochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: immunocytochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:12:39Z
definition: A type of immunocytochemistry evidence that is used in a manual assertion.
id: ECO:0001186
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of immunocytochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: immunocytochemistry evidence used in manual assertion
annotated property: definition

histology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: histology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: histology evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:09:50Z
definition: A type of histology evidence that is used in a manual assertion.
id: ECO:0001187
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of histology evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: histology evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: histology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated source: histology evidence used in manual assertion
annotated target: imaging assay evidence used in manual assertion
annotated property: subClassOf

immunodepletion evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunodepletion evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: immunodepletion evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:16:19Z
definition: A type of immunodepletion evidence that is used in a manual assertion.
id: ECO:0001188
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of immunodepletion evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: immunodepletion evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: immunodepletion evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: immunodepletion evidence used in manual assertion
annotated property: subClassOf

immunohistochemistry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunohistochemistry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: immunohistochemistry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:17:22Z
definition: A type of immunohistochemistry evidence that is used in a manual assertion.
id: ECO:0001189
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of immunohistochemistry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: immunohistochemistry evidence used in manual assertion
annotated property: definition

in vitro acetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro acetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro acetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:19:24Z
definition: A type of in vitro acetylation assay evidence that is used in a manual assertion.
id: ECO:0001190
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro acetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro cleavage assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro cleavage assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro cleavage assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:21:22Z
definition: A type of in vitro cleavage assay evidence that is used in a manual assertion.
id: ECO:0001191
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vitro cleavage assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro cleavage assay evidence used in manual assertion
annotated property: definition

in vitro deacetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro deacetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro deacetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:23:42Z
definition: A type of in vitro deacetylation assay evidence that is used in a manual assertion.
id: ECO:0001192
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro deacetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro defarnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro defarnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro defarnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:25:45Z
definition: A type of in vitro defarnesylation assay evidence that is used in a manual assertion.
id: ECO:0001193
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vitro defarnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro defarnesylation assay evidence used in manual assertion
annotated property: definition

in vitro demethylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro demethylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro demethylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:27:00Z
definition: A type of in vitro demethylation assay evidence that is used in a manual assertion.
id: ECO:0001194
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro demethylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro demethylation assay evidence used in manual assertion
annotated property: subClassOf

in vitro desumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro desumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro desumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:29:01Z
definition: A type of in vitro desumoylation assay evidence that is used in a manual assertion.
id: ECO:0001195
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vitro desumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vitro desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro deubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro deubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro deubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:29:48Z
definition: A type of in vitro deubiquitination assay evidence that is used in a manual assertion.
id: ECO:0001196
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro deubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro farnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro farnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro farnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:30:50Z
definition: A type of in vitro farnesylation assay evidence that is used in a manual assertion.
id: ECO:0001197
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vitro farnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro farnesylation assay evidence used in manual assertion
annotated property: definition

in vitro methylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro methylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro methylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:31:54Z
definition: A type of in vitro methylation assay evidence that is used in a manual assertion.
id: ECO:0001198
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro methylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro palmitoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro palmitoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro palmitoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:32:57Z
definition: A type of in vitro palmitoylation assay evidence that is used in a manual assertion.
id: ECO:0001199
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro palmitoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro phosphatase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro phosphatase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro phosphatase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:34:16Z
definition: A type of in vitro phosphatase assay evidence that is used in a manual assertion.
id: ECO:0001200
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro phosphatase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro polyADP-ribosylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro polyADP-ribosylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro polyADP-ribosylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:34:57Z
definition: A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion.
id: ECO:0001201
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro protein kinase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro protein kinase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro protein kinase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:39:57Z
definition: A type of in vitro protein kinase assay evidence that is used in a manual assertion.
id: ECO:0001202
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vitro protein kinase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro protein kinase assay evidence used in manual assertion
annotated property: definition

in vitro sumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro sumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro sumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:41:25Z
definition: A type of in vitro sumoylation assay evidence that is used in a manual assertion.
id: ECO:0001203
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vitro sumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro sumoylation assay evidence used in manual assertion
annotated property: definition

in vitro transcription assay used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro transcription assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro transcription assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:44:40Z
definition: A type of in vitro transcription assay evidence that is used in a manual assertion.
id: ECO:0001204
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro transcription assay used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vitro transcription assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro transcription assay used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vitro transcription assay used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro translation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro translation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro translation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:46:30Z
definition: A type of in vitro translation assay evidence that is used in a manual assertion.
id: ECO:0001205
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vitro translation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vitro translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vitro ubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vitro ubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vitro ubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:47:18Z
definition: A type of in vitro ubiquitination assay evidence that is used in a manual assertion.
id: ECO:0001206
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vitro ubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vitro ubiquitination assay evidence used in manual assertion
annotated property: subClassOf

in vivo acetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo acetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo acetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:48:56Z
definition: A type of in vivo acetylation assay evidence that is used in manual assertion.
id: ECO:0001207
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo acetylation assay evidence that is used in manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo acetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo cleavage assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo cleavage assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo cleavage assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:49:43Z
definition: A type of in vivo cleavage assay evidence that is used in a manual assertion.
id: ECO:0001208
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo cleavage assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo cleavage assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo deacetylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo deacetylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo deacetylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:50:47Z
definition: A type of in vivo deacetylation assay evidence that is used in a manual assertion.
id: ECO:0001209
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo deacetylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo deacetylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo defarnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo defarnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo defarnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:52:41Z
definition: A type of in vivo defarnesylation assay evidence that is used in a manual assertion.
id: ECO:0001210
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vivo defarnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo defarnesylation assay evidence used in manual assertion
annotated property: definition

in vivo demethylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo demethylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo demethylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:53:40Z
definition: A type of in vivo demethylation assay evidence that is used in a manual assertion.
id: ECO:0001211
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo demethylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo demethylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo desumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo desumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo desumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:54:36Z
definition: A type of in vivo desumoylation assay evidence that is used in a manual assertion.
id: ECO:0001212
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo desumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo desumoylation assay evidence used in manual assertion
annotated property: subClassOf

in vivo deubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo deubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo deubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:56:28Z
definition: A type of in vivo deubiquitination assay evidence that is used in a manual assertion.
id: ECO:0001213
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo deubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo deubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo farnesylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo farnesylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo farnesylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T17:57:18Z
definition: A type of in vivo farnesylation assay evidence that is used in a manual assertion.
id: ECO:0001214
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vivo farnesylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo farnesylation assay evidence used in manual assertion
annotated property: subClassOf

in vivo methylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo methylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo methylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:03:16Z
definition: A type of in vivo methylation assay evidence that is used in a manual assertion.
id: ECO:0001215
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo methylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo methylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo palmitoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo palmitoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo palmitoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:04:07Z
definition: A type of in vivo palmitoylation assay evidence that is used in a manual assertion.
id: ECO:0001216
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo palmitoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo palmitoylation assay evidence used in manual assertion
annotated property: definition

in vivo phosphatase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo phosphatase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo phosphatase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:06:08Z
definition: A type of in vivo phosphatase assay evidence that is used in a manual assertion.
id: ECO:0001217
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo phosphatase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo phosphatase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo protein kinase assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo protein kinase assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo protein kinase assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:07:58Z
definition: A type of in vivo protein kinase assay evidence that is used in a manual assertion.
id: ECO:0001218
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo protein kinase assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo protein kinase assay evidence used in manual assertion
annotated property: definition

in vivo sumoylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo sumoylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo sumoylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:09:49Z
definition: A type of in vivo sumoylation assay evidence that is used in a manual assertion.
id: ECO:0001219
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of in vivo sumoylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo sumoylation assay evidence used in manual assertion
annotated property: subClassOf

in vivo transcription assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo transcription assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo transcription assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:11:20Z
definition: A type of in vivo transcription assay evidence that is used in a manual assertion.
id: ECO:0001220
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo transcription assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo transcription assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo translation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo translation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo translation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:42:50Z
definition: A type of in vivo translation assay evidence that is used in a manual assertion.
id: ECO:0001221
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo translation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo translation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

in vivo ubiquitination assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo ubiquitination assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo ubiquitination assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:44:11Z
definition: A type of in vivo ubiquitination assay evidence that is used in a manual assertion.
id: ECO:0001222
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of in vivo ubiquitination assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: in vivo ubiquitination assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

induced mutation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: induced mutation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: induced mutation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:45:56Z
definition: A type of induced mutation evidence that is used in a manual assertion.
id: ECO:0001223
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of induced mutation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: induced mutation evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: induced mutation evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: induced mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

knockin evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: knockin evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: knockin evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:49:35Z
definition: A type of knockin evidence that is used in a manual assertion.
id: ECO:0001224
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: knockin evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: knockin evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of knockin evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: knockin evidence used in manual assertion
annotated property: definition

knockout evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: knockout evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: knockout evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:51:31Z
definition: A type of knockout evidence that is used in a manual assertion.
id: ECO:0001225
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of knockout evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: knockout evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: knockout evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: knockout evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

lipid binding assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: lipid binding assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: lipid binding assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:52:19Z
definition: A type of lipid binding assay evidence that is used in a manual assertion.
id: ECO:0001226
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: lipid binding assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of lipid binding assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: lipid binding assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: lipid binding assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

luminescence-based mammalian interactome mapping assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: luminescence-based mammalian interactome mapping assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: luminescence-based mammalian interactome mapping assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:55:16Z
definition: A type of LUMIER assay evidence that is used in a manual assertion.
id: ECO:0001227
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of LUMIER assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: luminescence-based mammalian interactome mapping assay evidence used in manual assertion
annotated property: definition

macroscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: macroscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: macroscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:56:21Z
definition: A type of macroscopy evidence that is used in a manual assertion.
id: ECO:0001228
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: macroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: macroscopy evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of macroscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: macroscopy evidence used in manual assertion
annotated property: definition

mammalian 2-hybrid assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mammalian 2-hybrid assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: mammalian 2-hybrid assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:57:25Z
definition: A type of mammalian 2-hybrid assay evidence that is used in a manual assertion.
id: ECO:0001229
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of mammalian 2-hybrid assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: mammalian 2-hybrid assay evidence used in manual assertion
annotated property: definition

mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: mass spectrometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T18:59:50Z
definition: A type of mass spectrometry evidence that is used in a manual assertion.
id: ECO:0001230
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: mass spectrometry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: mass spectrometry evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

medical imaging evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: medical imaging evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: medical imaging evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:04:43Z
definition: A type of medical imaging evidence that is used in a manual assertion.
id: ECO:0001231
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: medical imaging evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of medical imaging evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: medical imaging evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: medical imaging evidence used in manual assertion
annotated target: imaging assay evidence used in manual assertion
annotated property: subClassOf

microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence
subclass of: imaging assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:05:37Z
definition: A type of microscopy evidence that is used in a manual assertion.
id: ECO:0001232
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: microscopy evidence used in manual assertion
annotated target: imaging assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: microscopy evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

motility wound healing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motility wound healing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: motility wound healing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:08:13Z
definition: A type of motility wound healing assay evidence that is used in a manual assertion.
id: ECO:0001233
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of motility wound healing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: motility wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

MTS assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: MTS assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: MTS assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:08:51Z
definition: A type of MTS assay evidence that is used in a manual assertion.
id: ECO:0001234
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: MTS assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: MTS assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of MTS assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: MTS assay evidence used in manual assertion
annotated property: definition

MTT assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: MTT assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: MTT assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:13:17Z
definition: A type of MTT assay evidence that is used in a manual assertion.
id: ECO:0001235
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: MTT assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of MTT assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: MTT assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: MTT assay evidence used in manual assertion
annotated property: subClassOf

multiplex bead-based immunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: multiplex bead-based immunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: multiplex bead-based immunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:14:03Z
definition: A type of multiplex bead-based immunoassay evidence that is used in a manual assertion.
id: ECO:0001236
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of multiplex bead-based immunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: multiplex bead-based immunoassay evidence used in manual assertion
annotated property: definition

natural variation mutant evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: natural variation mutant evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: natural variation mutant evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:15:48Z
definition: A type of natural variation mutant evidence that is used in a manual assertion.
id: ECO:0001237
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: natural variation mutant evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: natural variation mutant evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of natural variation mutant evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: natural variation mutant evidence used in manual assertion
annotated property: definition

nuclear magnetic resonance spectroscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclear magnetic resonance spectroscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: nuclear magnetic resonance spectroscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:18:50Z
definition: A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion.
id: ECO:0001238
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: nuclear magnetic resonance spectroscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

nuclear fragmentation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclear fragmentation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: nuclear fragmentation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:19:55Z
definition: A type of nuclear fragmentation evidence that is used in a manual assertion.
id: ECO:0001239
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of nuclear fragmentation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: nuclear fragmentation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

nuclease protection assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nuclease protection assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: nuclease protection assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:23:41Z
definition: A type of nuclease protection assay evidence that is used in a manual assertion.
id: ECO:0001240
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of nuclease protection assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: nuclease protection assay evidence used in manual assertion
annotated property: definition

nucleotide analog incorporation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: nucleotide analog incorporation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: nucleotide analog incorporation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:25:17Z
definition: A type of nucleotide analog incorporation evidence that is used in a manual assertion.
id: ECO:0001241
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of nucleotide analog incorporation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: nucleotide analog incorporation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

phage display evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phage display evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: phage display evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:27:17Z
definition: A type of phage display evidence that is used in a manual assertion.
id: ECO:0001242
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: phage display evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of phage display evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: phage display evidence used in manual assertion
annotated property: definition

phosphoamino acid analysis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: phosphoamino acid analysis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: phosphoamino acid analysis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:28:59Z
definition: A type of phosphoamino acid analysis evidence that is used in a manual assertion.
id: ECO:0001243
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of phosphoamino acid analysis evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: phosphoamino acid analysis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

peptide affinity enrichment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: peptide affinity enrichment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: peptide affinity enrichment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T19:30:13Z
definition: A type of peptide affinity enrichment evidence that is used in a manual assertion.
id: ECO:0001244
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of peptide affinity enrichment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: peptide affinity enrichment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

peptide array evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: peptide array evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: peptide array evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:16:45Z
definition: A type of peptide array evidence that is used in a manual assertion.
id: ECO:0001245
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: peptide array evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: peptide array evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of peptide array evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: peptide array evidence used in manual assertion
annotated property: definition

physical examination evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: physical examination evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: physical examination evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:17:27Z
definition: A type of physical examination evidence that is used in a manual assertion.
id: ECO:0001246
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: physical examination evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: physical examination evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of physical examination evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: physical examination evidence used in manual assertion
annotated property: definition

point mutation evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: point mutation evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: point mutation evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:18:10Z
definition: A type of point mutation evidence that is used in a manual assertion.
id: ECO:0001247
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: point mutation evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: point mutation evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of point mutation evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: point mutation evidence used in manual assertion
annotated property: definition

propidium iodide staining evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: propidium iodide staining evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: propidium iodide staining evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:18:58Z
definition: A type of propidium iodide staining evidence that is used in a manual assertion.
id: ECO:0001248
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of propidium iodide staining evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: propidium iodide staining evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

fluorescence evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: fluorescence evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: fluorescence evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:19:35Z
definition: A type of protein detection by fluorescence evidence that is used in a manual assertion.
id: ECO:0001249
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of protein detection by fluorescence evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: fluorescence evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: fluorescence evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: fluorescence evidence used in manual assertion
annotated property: subClassOf

protein dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:20:30Z
definition: A type of protein dot blot assay evidence that is used in a manual assertion.
id: ECO:0001250
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of protein dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: protein dot blot assay evidence used in manual assertion
annotated property: definition

protein microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:21:11Z
definition: A type of protein microarray evidence that is used in a manual assertion.
id: ECO:0001251
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of protein microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: protein microarray evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: protein microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: protein microarray evidence used in manual assertion
annotated property: subClassOf

protein sequencing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: protein sequencing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: protein sequencing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:22:08Z
definition: A type of protein sequencing assay evidence that is used in a manual assertion.
id: ECO:0001252
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of protein sequencing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: protein sequencing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

quantitative mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: quantitative mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: quantitative mass spectrometry evidence
subclass of: mass spectrometry evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:23:15Z
definition: A type of quantitative mass spectrometry evidence that is used in a manual assertion.
id: ECO:0001253
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: mass spectrometry evidence used in manual assertion
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of quantitative mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: quantitative mass spectrometry evidence used in manual assertion
annotated property: definition

radioisotope assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: radioisotope assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: radioisotope assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:27:05Z
definition: A type of radioisotope assay evidence that is used in a manual assertion.
id: ECO:0001254
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: radioisotope assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of radioisotope assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: radioisotope assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: radioisotope assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

radioimmunoassay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: radioimmunoassay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: radioimmunoassay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:28:04Z
definition: A type of radioimmunoassay evidence that is used in a manual assertion.
id: ECO:0001255
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of radioimmunoassay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: radioimmunoassay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

imaging assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: imaging assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: imaging assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:00Z
definition: A type of imaging assay evidence that is used in a manual assertion.
id: ECO:0001256
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of imaging assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: imaging assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: imaging assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: imaging assay evidence used in manual assertion
annotated property: subClassOf

restriction fragment detection evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: restriction fragment detection evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: restriction fragment detection evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:05Z
definition: A type of restriction fragment detection evidence that is used in a manual assertion.
id: ECO:0001257
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of restriction fragment detection evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: restriction fragment detection evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

spectrophotometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: spectrophotometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: spectrophotometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:09Z
definition: A type of spectrophotometry evidence that is used in a manual assertion.
id: ECO:0001258
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: spectrophotometry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of spectrophotometry evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: spectrophotometry evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: spectrophotometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

syngeneic transplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: syngeneic transplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: syngeneic transplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:13Z
definition: A type of syngeneic transplantation experiment evidence that is used in a manual assertion.
id: ECO:0001259
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of syngeneic transplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: syngeneic transplantation experiment evidence used in manual assertion
annotated property: subClassOf

xenotransplantation experiment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: xenotransplantation experiment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: xenotransplantation experiment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:18Z
definition: A type of xenotransplantation experiment evidence that is used in a manual assertion.
id: ECO:0001260
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of xenotransplantation experiment evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: xenotransplantation experiment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

WST-1 assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: WST-1 assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: WST-1 assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:22Z
definition: A type of WST-1 assay evidence that is used in a manual assertion.
id: ECO:0001261
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of WST-1 assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: WST-1 assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: WST-1 assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: WST-1 assay evidence used in manual assertion
annotated property: subClassOf

wound healing assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: wound healing assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: wound healing assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:26Z
definition: A type of wound healing assay evidence that is used in a manual assertion.
id: ECO:0001262
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: wound healing assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of wound healing assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: wound healing assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: wound healing assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

urine test evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: urine test evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: urine test evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:30Z
definition: A type of urine test evidence that is used in a manual assertion.
id: ECO:0001263
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: urine test evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of urine test evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: urine test evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: urine test evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:34Z
definition: A type of TUNEL assay evidence that is used in a manual assertion.
id: ECO:0001264
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of TUNEL assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

tryptic phosphopeptide mapping assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: tryptic phosphopeptide mapping assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: tryptic phosphopeptide mapping assay evidence
subclass of: mass spectrometry evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:38Z
definition: A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion.
id: ECO:0001265
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: mass spectrometry evidence used in manual assertion
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: tryptic phosphopeptide mapping assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

transgenic organism evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: transgenic organism evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: transgenic organism evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:41Z
definition: A type of transgenic organism evidence that is used in a manual assertion.
id: ECO:0001266
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of transgenic organism evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: transgenic organism evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: transgenic organism evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: transgenic organism evidence used in manual assertion
annotated property: subClassOf

tissue microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: tissue microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: array experiment evidence used in manual assertion
subclass of: tissue microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:45Z
definition: A type of tissue microarray evidence that is used in a manual assertion.
id: ECO:0001267
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: tissue microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: array experiment evidence used in manual assertion
annotated source: tissue microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of tissue microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: tissue microarray evidence used in manual assertion
annotated property: definition

TACE activity assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: TACE activity assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: TACE activity assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:49Z
definition: A type of TACE activity assay evidence that is used in a manual assertion.
id: ECO:0001268
has_obo_namespace: eco
created by: mchibucos

Axiom
annotated target: A type of TACE activity assay evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: TACE activity assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: TACE activity assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: TACE activity assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

surface plasmon resonance evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: surface plasmon resonance evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: physical interaction evidence used in manual assertion
subclass of: surface plasmon resonance evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:54Z
definition: A type of surface plasmon resonance evidence that is used in a manual assertion.
id: ECO:0001269
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: physical interaction evidence used in manual assertion
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of surface plasmon resonance evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: surface plasmon resonance evidence used in manual assertion
annotated property: definition

restriction landmark genomic scanning evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: restriction landmark genomic scanning evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: restriction landmark genomic scanning evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:29:58Z
definition: A type of restriction landmark genome scanning evidence that is used in a manual assertion.
id: ECO:0001270
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of restriction landmark genome scanning evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: restriction landmark genomic scanning evidence used in manual assertion
annotated property: definition

resonant mirror biosensor evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: resonant mirror biosensor evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: resonant mirror biosensor evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T20:30:02Z
definition: A type of resonant mirror biosensor evidence that is used in a manual assertion.
id: ECO:0001271
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of resonant mirror biosensor evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: resonant mirror biosensor evidence used in manual assertion
annotated property: definition

high-performance liquid chromatography evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: high-performance liquid chromatography evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: high-performance liquid chromatography evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-16T20:49:54Z
definition: A type of high-performance liquid chromatography evidence that is used in a manual assertion.
id: ECO:0001272
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of high-performance liquid chromatography evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: high-performance liquid chromatography evidence used in manual assertion
annotated property: definition

ectopic expression evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: ectopic expression evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: ectopic expression evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2014-03-17T22:34:56Z
definition: A type of ectopic expression evidence that is used in a manual assertion.
id: ECO:0001273
has_obo_namespace: eco
created by: mchibucos

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: ectopic expression evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: ectopic expression evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of ectopic expression evidence that is used in a manual assertion.
database_cross_reference: ECO:MCC
annotated source: ectopic expression evidence used in manual assertion
annotated property: definition

alkaline phosphatase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-29T15:53:45Z
definition: A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing.
comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane.
id: ECO:0001801
has_related_synonym: SEAP reporter assay
has_related_synonym: Secreted Embryonic Alkaline Phosphatase reporter assay
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11823238
annotated source: alkaline phosphatase reporter gene assay evidence
annotated property: definition

beta-galactosidase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-29T15:57:10Z
definition: A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.
id: ECO:0001802
comment: The assay is often performed using a plasmid borne construction on a lacZ strain.
has_exact_synonym: beta-gal reporter gene assay
has_obo_namespace: eco
has_related_synonym: lacZ reporter gene assay
created by: swolfish

Axiom
annotated target: A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20439410
annotated source: beta-galactosidase reporter gene assay evidence
annotated property: definition

chloramphenicol acetyltransferase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-29T16:06:42Z
definition: A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic.
has_exact_synonym: CAT reporter gene assay
id: ECO:0001803
comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: CAT reporter gene assay
database_cross_reference: PMID:1630936
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic.
database_cross_reference: ECO:SW
database_cross_reference: PMID:1630936
annotated source: chloramphenicol acetyltransferase reporter gene assay evidence
annotated property: definition

beta-glucuronidase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-29T16:11:02Z
definition: A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection.
id: ECO:0001804
has_exact_synonym: GUS reporter gene assay
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection.
database_cross_reference: ECO:SW
annotated source: beta-glucuronidase reporter gene assay evidence
annotated property: definition

luciferase reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-29T16:15:39Z
definition: A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence.
id: ECO:0001805
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17623934
annotated source: luciferase reporter gene assay evidence
annotated property: definition

chromatin immunoprecipitation- exonuclease evidence
type: Class
subclass of: chromatin immunoprecipitation evidence
creation date: 2015-10-29T16:37:36Z
definition: A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data.
has_exact_synonym: ChIP-exo
id: ECO:0001806
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data.
database_cross_reference: ECO:SW
database_cross_reference: PMID:25249628
annotated source: chromatin immunoprecipitation- exonuclease evidence
annotated property: definition

Axiom
annotated target: ChIP-exo
database_cross_reference: PMID:25249628
annotated source: chromatin immunoprecipitation- exonuclease evidence
annotated property: has_exact_synonym

electrophoretic mobility shift assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: electrophoretic mobility shift assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: electrophoretic mobility shift assay evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-11-03T12:13:17Z
definition: A type of electrophoretic mobility shift that is used in a manual assertion.
id: ECO:0001807
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of electrophoretic mobility shift that is used in a manual assertion.
database_cross_reference: ECO:SW
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: electrophoretic mobility shift assay evidence used in manual assertion
annotated property: subClassOf

reverse transcription polymerase chain reaction evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: reverse transcription polymerase chain reaction evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: reverse transcription polymerase chain reaction evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-11-03T12:22:46Z
definition: A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion.
id: ECO:0001808
has_obo_namespace: eco
created by: swolfish

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion.
database_cross_reference: ECO:SW
annotated source: reverse transcription polymerase chain reaction evidence used in manual assertion
annotated property: definition

DNA affinity chromatography evidence
type: Class
subclass of: chromatography evidence
creation date: 2015-11-03T13:48:49Z
definition: A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins.
comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture.
has_related_synonym: DNA affinity purification
id: ECO:0001809
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11694305
database_cross_reference: PMID:11725488
annotated source: DNA affinity chromatography evidence
annotated property: definition

DNAse footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
creation date: 2015-11-03T13:55:15Z
definition: A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions.
has_related_synonym: DNase protection
id: ECO:0001810
comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions.
database_cross_reference: ECO:SW
database_cross_reference: PMID:212715
database_cross_reference: PMID:22194258
annotated source: DNAse footprinting evidence
annotated property: definition

fluorescence anisotropy evidence
type: Class
subclass of: fluorescence evidence
creation date: 2015-11-03T14:01:36Z
definition: A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules.
id: ECO:0001811
has_exact_synonym: FA
has_related_synonym: FP
has_related_synonym: Fluorescence polarization
comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: Fluorescence polarization
database_cross_reference: PMCID:3277431
database_cross_reference: PMID:20232898
annotated source: fluorescence anisotropy evidence
annotated property: has_related_synonym

Axiom
annotated target: A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20232898
annotated source: fluorescence anisotropy evidence
annotated property: definition

Axiom
annotated target: FP
database_cross_reference: PMCID:3277431
database_cross_reference: PMID:20232898
annotated source: fluorescence anisotropy evidence
annotated property: has_related_synonym

genomic systematic evolution of ligands by exponential amplification evidence
type: Class
subclass of: systematic evolution of ligands by exponential amplification evidence
creation date: 2015-11-03T14:05:37Z
definition: A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing.
id: ECO:0001812
comment: The reported sequences should always be verified for presence in the genome sequence.
has_obo_namespace: eco
has_exact_synonym: genomic SELEX
created by: swolfish

Axiom
annotated target: A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing.
database_cross_reference: ECO:SW
database_cross_reference: PMID:20541015
database_cross_reference: PMID:21720957
annotated source: genomic systematic evolution of ligands by exponential amplification evidence
annotated property: definition

Axiom
database_cross_reference: PMID:20541015
annotated target: genomic SELEX
annotated source: genomic systematic evolution of ligands by exponential amplification evidence
annotated property: has_exact_synonym

heteronuclear single quantum coherence spectroscopy evidence
type: Class
subclass of: nuclear magnetic resonance spectroscopy evidence
creation date: 2015-11-03T14:11:06Z
definition: A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum.
comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site.
id: ECO:0001813
has_exact_synonym: HSQC
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: HSQC
database_cross_reference: PMID:19856946
annotated source: heteronuclear single quantum coherence spectroscopy evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum.
database_cross_reference: ECO:SW
database_cross_reference: PMID:19856946
annotated source: heteronuclear single quantum coherence spectroscopy evidence
annotated property: definition

methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
creation date: 2015-11-03T14:24:04Z
definition: A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA.
id: ECO:0001814
has_related_synonym: MPE Fe(II) footprinting
has_related_synonym: MPE-EDTA Fe(II) footprinting
has_related_synonym: Methidiumpropyl-EDTA Fe(II) footprinting
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: Methidiumpropyl-EDTA Fe(II) footprinting
database_cross_reference: PMID:6225070
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym

Axiom
annotated target: MPE Fe(II) footprinting
database_cross_reference: PMID:6225070
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym

Axiom
annotated target: MPE-EDTA Fe(II) footprinting
database_cross_reference: PMID:6225070
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: has_related_synonym

Axiom
annotated target: A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:6225070
annotated source: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence
annotated property: definition

copper-phenanthroline footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
has_exact_synonym: 1,10-Phenanthroline-copper footprinting
creation date: 2015-11-04T08:37:51Z
definition: A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts.
id: ECO:0001815
has_related_synonym: OP-Cu Complex
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: 1,10-Phenanthroline-copper footprinting
database_cross_reference: PMID:1384472
annotated source: copper-phenanthroline footprinting evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11691942
annotated source: copper-phenanthroline footprinting evidence
annotated property: definition

Axiom
annotated target: OP-Cu Complex
database_cross_reference: PMID:1384472
annotated source: copper-phenanthroline footprinting evidence
annotated property: has_related_synonym

green fluorescent protein reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-11-04T08:58:42Z
definition: A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light.
id: ECO:0001816
has_related_synonym: GFP promoter fusion
has_exact_synonym: GFP reporter gene assay
has_obo_namespace: eco
has_related_synonym: green fluorescent protein promoter fusion
created by: swolfish

Axiom
annotated target: A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light.
database_cross_reference: ECO:SW
database_cross_reference: PMID:11989662
annotated source: green fluorescent protein reporter gene assay evidence
annotated property: definition

Axiom
annotated target: GFP reporter gene assay
database_cross_reference: PMID:11989662
annotated source: green fluorescent protein reporter gene assay evidence
annotated property: has_exact_synonym

glutathione S-transferase pull-down assay evidence
type: Class
subclass of: protein binding evidence
creation date: 2015-11-04T09:05:56Z
definition: A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions.
id: ECO:0001817
has_exact_synonym: GST pull-down assay
comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions.
database_cross_reference: ECO:SW
database_cross_reference: PMID:26096507
annotated source: glutathione S-transferase pull-down assay evidence
annotated property: definition

Axiom
annotated target: GST pull-down assay
database_cross_reference: PMID:26096507
annotated source: glutathione S-transferase pull-down assay evidence
annotated property: has_exact_synonym

hydroxyl-radical footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
creation date: 2015-11-04T09:20:26Z
definition: A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel.
id: ECO:0001818
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel.
database_cross_reference: ECO:SW
database_cross_reference: PMID:18546600
database_cross_reference: PMID:19378159
database_cross_reference: PMID:3090544
annotated source: hydroxyl-radical footprinting evidence
annotated property: definition

primer extension assay evidence
type: Class
subclass of: transcript expression level evidence
creation date: 2015-11-04T09:33:18Z
definition: A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA.
id: ECO:0001819
comment: The most commonly used radiolabel is 32P.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23378648
annotated source: primer extension assay evidence
annotated property: definition

rapid amplification of cDNA ends polymerase chain reaction evidence
type: Class
subclass of: polymerase chain reaction evidence
creation date: 2015-11-04T09:48:38Z
definition: A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript.
id: ECO:0001820
has_exact_synonym: RACE PCR
comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression.
has_obo_namespace: eco
created by: swolfish

Axiom
database_cross_reference: PMI:17498297
annotated target: RACE PCR
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17498297
annotated source: rapid amplification of cDNA ends polymerase chain reaction evidence
annotated property: definition

RNA sequencing assay evidence
type: Class
subclass of: nucleotide sequencing assay evidence
creation date: 2015-11-04T10:15:57Z
definition: A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms.
id: ECO:0001821
has_exact_synonym: RNA-Seq
has_exact_synonym: WTTS
has_exact_synonym: Whole Transcriptome Shotgun Sequencing
has_obo_namespace: eco
created by: swolfish

Axiom
database_cross_reference: PMID:23851085
annotated target: RNA-Seq
annotated source: RNA sequencing assay evidence
annotated property: has_exact_synonym

Axiom
database_cross_reference: PMID:23851085
annotated target: Whole Transcriptome Shotgun Sequencing
annotated source: RNA sequencing assay evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms.
database_cross_reference: ECO:SW
database_cross_reference: PMID:19015660
annotated source: RNA sequencing assay evidence
annotated property: definition

survival analysis evidence
type: Class
subclass of: knockout evidence
creation date: 2015-11-04T10:48:59Z
definition: A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter.
id: ECO:0001822
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter.
database_cross_reference: ECO:SW
annotated source: survival analysis evidence
annotated property: definition

x-ray crystallography evidence
type: Class
subclass of: crystallography evidence
creation date: 2015-11-04T10:56:22Z
definition: A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules.
id: ECO:0001823
comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules.
database_cross_reference: ECO:SW
database_cross_reference: PMID:23135450
database_cross_reference: PMID:24648090
annotated source: x-ray crystallography evidence
annotated property: definition

DNA adenine methyltransferase identification evidence
type: Class
subclass of: affinity evidence
creation date: 2015-11-05T15:56:13Z
definition: A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site.
has_exact_synonym: DamID
id: ECO:0001824
comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII.
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: DamID
database_cross_reference: PMID:19588092
annotated source: DNA adenine methyltransferase identification evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site.
database_cross_reference: ECO:SW
database_cross_reference: PMID:16938559
database_cross_reference: PMID:17545983
database_cross_reference: PMID:19588092
annotated source: DNA adenine methyltransferase identification evidence
annotated property: definition

isothermal titration calorimetry evidence
type: Class
subclass of: affinity evidence
creation date: 2015-11-05T16:06:20Z
definition: A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction.
id: ECO:0001825
has_exact_synonym: ITC
comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)).
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: ITC
database_cross_reference: PMID:10527727
annotated source: isothermal titration calorimetry evidence
annotated property: has_exact_synonym

Axiom
annotated target: A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10527727
annotated source: isothermal titration calorimetry evidence
annotated property: definition

ultraviolet light footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
creation date: 2015-11-05T16:13:36Z
definition: A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts.
id: ECO:0001826
has_related_synonym: UV footprinting
has_obo_namespace: eco
created by: swolfish
has_related_synonym: ultraviolet footprinting

Axiom
annotated target: A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts.
database_cross_reference: ECO:SW
database_cross_reference: PMID:2842760
database_cross_reference: PMID:7602584
annotated source: ultraviolet light footprinting evidence
annotated property: definition

premethylation interference footprinting evidence
type: Class
subclass of: nucleic acid binding evidence
creation date: 2015-11-05T16:16:27Z
definition: A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel.
id: ECO:0001827
has_obo_namespace: eco
has_related_synonym: methylation interference footprinting
created by: swolfish

Axiom
annotated target: A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel.
database_cross_reference: ECO:SW
database_cross_reference: PMID:1583685
annotated source: premethylation interference footprinting evidence
annotated property: definition

visual sequence inspection evidence
type: Class
subclass of: inference from background scientific knowledge used in manual assertion
creation date: 2015-11-10T14:40:16Z
definition: A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites.
id: ECO:0001828
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites.
database_cross_reference: ECO:SW
annotated source: visual sequence inspection evidence
annotated property: definition

ferric uptake regulator titration assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-11-10T14:55:36Z
definition: A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.
id: ECO:0001829
has_exact_synonym: FURTA
has_related_synonym: Fluorescence polarization
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: FURTA
database_cross_reference: PMID:10713425
annotated source: ferric uptake regulator titration assay evidence
annotated property: has_exact_synonym

Axiom
annotated target: Fluorescence polarization
database_cross_reference: PMID:15689115
annotated source: ferric uptake regulator titration assay evidence
annotated property: has_related_synonym

Axiom
annotated target: A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10713425
database_cross_reference: PMID:7642488
database_cross_reference: PMID:8107138
annotated source: ferric uptake regulator titration assay evidence
annotated property: definition

cell viability assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2015-02-13T12:08:01Z
id: ECO:0005004
has_obo_namespace: eco
created by: snadendla

cell proliferation assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2015-02-11T16:27:13Z
id: ECO:0005007
has_obo_namespace: eco
created by: snadendla

DNA synthesis cell proliferation assay evidence
type: Class
subclass of: cell proliferation assay evidence
creation date: 2015-02-11T16:30:10Z
id: ECO:0005008
has_obo_namespace: eco
created by: snadendla

metabolic cell proliferation assay evidence
type: Class
subclass of: cell proliferation assay evidence
creation date: 2015-02-11T16:36:33Z
id: ECO:0005009
has_obo_namespace: eco
created by: snadendla

cell proliferation marker detection assay evidence
type: Class
subclass of: cell proliferation assay evidence
creation date: 2015-02-11T16:37:23Z
id: ECO:0005010
has_obo_namespace: eco
created by: snadendla

ATP bioluminescence assay evidence
type: Class
subclass of: cell proliferation assay evidence
creation date: 2015-02-11T16:47:30Z
id: ECO:0005011
has_obo_namespace: eco
created by: snadendla

cytotoxicity assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2015-02-12T11:39:35Z
id: ECO:0005012
has_obo_namespace: eco
created by: snadendla

in vitro cell based assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2015-02-12T13:26:55Z
id: ECO:0005014
has_obo_namespace: eco
created by: snadendla

staining evidence
type: Class
subclass of: direct assay evidence
creation date: 2015-02-19T11:48:57Z
id: ECO:0005019
has_obo_namespace: eco
created by: snadendla

chemotaxis assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2015-02-19T13:10:20Z
id: ECO:0005021
has_obo_namespace: eco
created by: snadendla

genetic transformation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-03-16T15:33:50Z
id: ECO:0005027
has_obo_namespace: eco
created by: snadendla

structure determination evidence
type: Class
subclass of: experimental evidence
creation date: 2015-03-20T12:16:17Z
id: ECO:0005031
has_obo_namespace: eco
created by: snadendla

electron microscopy evidence
type: Class
subclass of: microscopy evidence
subclass of: structure determination evidence
creation date: 2015-03-20T12:21:48Z
id: ECO:0005033
has_obo_namespace: eco
created by: snadendla

apoptotic assay evidence
type: Class
subclass of: in vitro cell based assay evidence
creation date: 2015-04-07T21:58:24Z
id: ECO:0005034
has_obo_namespace: eco
created by: snadendla

in vivo polyADP-ribosylation assay evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2015-06-15T15:35:05Z
definition: A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo.
id: ECO:0005500
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo.
database_cross_reference: ECO:SN
database_cross_reference: PMID:2820766
database_cross_reference: SIB:PG
annotated source: in vivo polyADP-ribosylation assay evidence
annotated property: definition

in vivo polyADP-ribosylation assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: in vivo polyADP-ribosylation assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: direct assay evidence used in manual assertion
subclass of: in vivo polyADP-ribosylation assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-06-15T15:37:23Z
definition: A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion.
id: ECO:0005501
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: in vivo polyADP-ribosylation assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

ex vivo assay evidence
type: Class
subclass of: biological assay evidence
creation date: 2015-06-26T11:46:15Z
definition: A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions.
id: ECO:0005502
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions.
database_cross_reference: ECO:SN
database_cross_reference: PMID:16305312
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf
annotated source: ex vivo assay evidence
annotated property: definition

two-dimensional polyacrylamide gel electrophoresis evidence
type: Class
subclass of: gel electrophoresis evidence
creation date: 2015-10-01T16:23:20Z
has_exact_synonym: 2D PAGE
has_exact_synonym: 2D-PAGE
definition: A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape.
id: ECO:0005503
comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape.
database_cross_reference: ECO:SW
database_cross_reference: url:http://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html
annotated source: two-dimensional polyacrylamide gel electrophoresis evidence
annotated property: definition

spectrometry evidence
type: Class
subclass of: experimental evidence
creation date: 2015-08-14T15:48:08Z
id: ECO:0005504
has_obo_namespace: eco
created by: snadendla

regular expression motif search evidence
type: Class
subclass of: motif similarity evidence
creation date: 2015-10-09T13:51:52Z
definition: A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression.
id: ECO:0005505
comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667).
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17337630
annotated source: regular expression motif search evidence
annotated property: definition

missense mutation evidence
type: Class
subclass of: point mutation evidence
creation date: 2015-09-18T16:52:34Z
id: ECO:0005506
has_obo_namespace: eco
created by: snadendla

nonsense mutation evidence
type: Class
subclass of: point mutation evidence
creation date: 2015-09-18T16:55:32Z
id: ECO:0005507
has_obo_namespace: eco
created by: snadendla

silent mutation evidence
type: Class
subclass of: point mutation evidence
creation date: 2015-09-18T17:01:12Z
id: ECO:0005508
has_obo_namespace: eco
created by: snadendla

insertion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:02:03Z
id: ECO:0005509
has_obo_namespace: eco
created by: snadendla

deletion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:02:45Z
id: ECO:0005510
has_obo_namespace: eco
created by: snadendla

duplication mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:03:25Z
id: ECO:0005511
has_obo_namespace: eco
created by: snadendla

frameshift mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:05:49Z
id: ECO:0005512
has_obo_namespace: eco
created by: snadendla

repeat expansion mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:06:34Z
id: ECO:0005513
has_obo_namespace: eco
created by: snadendla

splice site mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:07:29Z
id: ECO:0005514
has_obo_namespace: eco
created by: snadendla

translocation mutation evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-18T17:08:01Z
id: ECO:0005515
has_obo_namespace: eco
created by: snadendla

molecule detection assay evidence
type: Class
subclass of: experimental evidence
creation date: 2015-09-24T15:54:21Z
id: ECO:0005516
has_obo_namespace: eco
created by: snadendla

protein detection assay evidence
type: Class
subclass of: molecule detection assay evidence
creation date: 2015-09-25T14:27:40Z
id: ECO:0005517
has_obo_namespace: eco
created by: snadendla

RNA detection assay evidence
type: Class
subclass of: molecule detection assay evidence
creation date: 2015-09-25T14:43:53Z
id: ECO:0005518
has_obo_namespace: eco
created by: snadendla

DNA detection assay evidence
type: Class
subclass of: molecule detection assay evidence
creation date: 2015-09-25T19:33:25Z
id: ECO:0005519
has_obo_namespace: eco
created by: snadendla

interferometric reflectance imaging sensor evidence
type: Class
subclass of: molecule detection assay evidence
creation date: 2015-09-28T16:01:01Z
definition: A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels.
comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively.
id: ECO:0005520
has_exact_synonym: IRIS
has_exact_synonym: SRIB
has_exact_synonym: Spectral Reflectance Imaging Biosensor
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21587155
database_cross_reference: PMID:24271115
annotated source: interferometric reflectance imaging sensor evidence
annotated property: definition

S1 nuclease protection assay evidence
type: Class
subclass of: nuclease protection assay evidence
creation date: 2015-09-28T17:16:56Z
definition: A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA.
id: ECO:0005521
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21390683
annotated source: S1 nuclease protection assay evidence
annotated property: definition

DNA dot blot assay evidence
type: Class
subclass of: DNA detection assay evidence
creation date: 2015-09-30T10:03:03Z
id: ECO:0005522
has_obo_namespace: eco
created by: snadendla

DNA dot blot assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA dot blot assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: DNA dot blot assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-09-30T10:04:00Z
definition: A type of DNA dot blot assay evidence that is used in a manual assertion.
id: ECO:0005523
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of DNA dot blot assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: DNA dot blot assay evidence used in manual assertion
annotated property: subClassOf

DNA microarray evidence
type: Class
subclass of: DNA detection assay evidence
creation date: 2015-09-30T10:12:48Z
definition: A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner.
has_related_synonym: DNA chip
has_related_synonym: DNA-array expression analysis
id: ECO:0005524
comment: The mRNA extraction is performed in induced and non-induced cells and reverse transcribed with different fluorophore labeling for controls and experiment. Fluorescence is measured at two different wavelengths and the ratio between the two fluorescence intensities corresponds to the induction level.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17660860
annotated source: DNA microarray evidence
annotated property: definition

Axiom
annotated target: DNA chip
database_cross_reference: PMID:10398077
annotated source: DNA microarray evidence
annotated property: has_related_synonym

DNA microarray evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: DNA microarray evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: DNA microarray evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-09-30T10:13:09Z
definition: A type of DNA microarray evidence that is used in a manual assertion.
id: ECO:0005525
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: DNA microarray evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: DNA microarray evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of DNA microarray evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW
annotated source: DNA microarray evidence used in manual assertion
annotated property: definition

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
type: Class
subclass of: protein detection assay evidence
creation date: 2015-09-30T10:51:56Z
definition: A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology.
id: ECO:0005526
comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available.
has_exact_synonym: MALDI-TOF Mass Spectrometry
has_exact_synonym: MALDI-TOF-MS
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21964792
database_cross_reference: PMID:25354905
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
annotated property: definition

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-09-30T10:58:51Z
definition: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion.
id: ECO:0005527
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: ECO:SW
annotated source: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion
annotated property: definition

site-directed mutagenesis evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-30T11:34:41Z
definition: A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence.
id: ECO:0005528
comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site.
has_obo_namespace: eco
has_exact_synonym: site directed mutagenesis
created by: snadendla

Axiom
annotated target: A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence.
database_cross_reference: ECO:SW
database_cross_reference: PMID:21204030
database_cross_reference: PMID:24011050
database_cross_reference: PMID:3541892
annotated source: site-directed mutagenesis evidence
annotated property: definition

random mutagenesis evidence
type: Class
subclass of: experimental phenotypic evidence
creation date: 2015-09-30T11:55:03Z
definition: A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis.
id: ECO:0005529
comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:15153637
database_cross_reference: PMID:18265275
annotated source: random mutagenesis evidence
annotated property: definition

random mutagenesis evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: random mutagenesis evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: experimental evidence used in manual assertion
subclass of: random mutagenesis evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-09-30T11:57:00Z
definition: A type of random mutagenesis evidence that is used in a manual assertion.
id: ECO:0005530
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: random mutagenesis evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: random mutagenesis evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

motif discovery evidence
type: Class
subclass of: multiple sequence alignment evidence
creation date: 2015-09-30T15:04:45Z
definition: A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs.
id: ECO:0005531
comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF (PMID: 19892760), from peaks in ChIP-seq experiments with the TF (PMID: 16264194), or from the comparative genomics analysis of the promoter regions of orthologs (PMID: 23032607).
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10812473
database_cross_reference: PMID:16845028
database_cross_reference: PMID:8211139
annotated source: motif discovery evidence
annotated property: definition

consensus search evidence
type: Class
subclass of: motif similarity evidence
creation date: 2015-10-09T14:12:54Z
definition: A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match.
id: ECO:0005532
comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match.
database_cross_reference: ECO:SW
database_cross_reference: PMID:15130839
annotated source: consensus search evidence
annotated property: definition

phylogenetic footprinting evidence
type: Class
subclass of: motif discovery evidence
creation date: 2015-09-30T16:23:02Z
definition: A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'.
id: ECO:0005533
comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340).
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'.
database_cross_reference: ECO:SW
database_cross_reference: PMID:16477324
database_cross_reference: PMID:3199442
annotated source: phylogenetic footprinting evidence
annotated property: definition

comparative genomics motif search evidence
type: Class
subclass of: motif similarity evidence
creation date: 2015-10-09T14:30:38Z
definition: A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes.
id: ECO:0005534
comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10854408
annotated source: comparative genomics motif search evidence
annotated property: definition

machine learning prediction of motif instance evidence
type: Class
subclass of: match to sequence model evidence
creation date: 2015-10-01T11:58:36Z
definition: A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences.
id: ECO:0005535
comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297).
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences.
database_cross_reference: ECO:SW
database_cross_reference: PMID:17117497
database_cross_reference: PMID:2014171
annotated source: machine learning prediction of motif instance evidence
annotated property: definition

position-specific scoring matrix motif search evidence
type: Class
subclass of: motif similarity evidence
creation date: 2015-10-09T15:04:35Z
definition: A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis.
id: ECO:0005536
has_narrow_synonym: PSSM
has_exact_synonym: PSSM motif search
comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457).
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis.
database_cross_reference: ECO:SW
database_cross_reference: PMID:10812473
annotated source: position-specific scoring matrix motif search evidence
annotated property: definition

xylE reporter gene assay evidence
type: Class
subclass of: reporter gene assay evidence
creation date: 2015-10-28T15:31:28Z
definition: A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene.
id: ECO:0005537
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene.
database_cross_reference: ECO:SW
database_cross_reference: PMID:2592344
annotated source: xylE reporter gene assay evidence
annotated property: definition

computationally derived logical inference
type: Class
creation date: 2015-11-17T15:15:50Z
id: ECO:0005538
has_obo_namespace: eco
created by: snadendla
deprecated: true

computationally derived logical inference used in automatic assertion
type: Class
creation date: 2015-11-17T15:17:47Z
id: ECO:0005539
has_obo_namespace: eco
created by: snadendla
deprecated: true

computationally derived logical inference from automatic assertion used in automatic assertion
type: Class
creation date: 2015-11-17T15:24:42Z
id: ECO:0005540
has_obo_namespace: eco
created by: snadendla
deprecated: true

computationally derived logical inference from manual assertion used in automatic assertion
type: Class
creation date: 2015-11-17T15:32:03Z
id: ECO:0005541
has_obo_namespace: eco
created by: snadendla
deprecated: true

biological system reconstruction evidence by experimental evidence from single species used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence by experimental evidence from single species
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence by experimental evidence from single species
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-12-16T15:32:41Z
definition: A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion.
id: ECO:0005542
comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof.
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: evidence used in manual assertion
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19450514
annotated source: biological system reconstruction evidence by experimental evidence from single species used in manual assertion
annotated property: definition

biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence by experimental evidence from mixed species
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence by experimental evidence from mixed species
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-12-16T15:45:01Z
definition: A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion.
id: ECO:0005543
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments.
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: evidence used in manual assertion
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22232657
annotated source: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion
annotated property: definition

biological system reconstruction evidence based on orthology evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on orthology evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence based on orthology evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-12-18T16:20:49Z
definition: A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion.
id: ECO:0005544
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: biological system reconstruction evidence based on orthology evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: biological system reconstruction evidence based on orthology evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: evidence used in manual assertion
annotated source: biological system reconstruction evidence based on orthology evidence used in manual assertion
annotated property: subClassOf

biological system reconstruction evidence based on orthology evidence
type: Class
subclass of: biological system reconstruction evidence based on homology evidence
creation date: 2015-12-16T15:57:37Z
definition: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species.
id: ECO:0005545
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128
annotated source: biological system reconstruction evidence based on orthology evidence
annotated property: definition

biological system reconstruction based on paralogy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on paralogy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence based on paralogy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-12-16T16:05:15Z
definition: A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion.
id: ECO:0005546
comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128
annotated source: biological system reconstruction based on paralogy evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: evidence used in manual assertion
annotated source: biological system reconstruction based on paralogy evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: biological system reconstruction based on paralogy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
type: Class
equivalent class: Class
  intersection of: biological system reconstruction evidence based on inference from background scientific knowledge
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: evidence used in manual assertion
subclass of: biological system reconstruction evidence based on inference from background scientific knowledge
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2015-12-16T16:07:42Z
definition: A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion.
id: ECO:0005547
comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components.
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17876790
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: evidence used in manual assertion
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion
annotated property: subClassOf

biological system reconstruction evidence based on inference from background scientific knowledge
type: Class
subclass of: biological system reconstruction evidence
creation date: 2015-12-18T15:32:15Z
definition: A type of biological system reconstruction based solely on background scientific knowledge of that biological system.
id: ECO:0005548
comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction based solely on background scientific knowledge of that biological system.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17876790
annotated source: biological system reconstruction evidence based on inference from background scientific knowledge
annotated property: definition

biological system reconstruction evidence based on homology evidence
type: Class
subclass of: biological system reconstruction evidence
creation date: 2015-12-18T15:49:46Z
definition: A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex.
id: ECO:0005549
comment: Inference may be based on paralogy or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128
annotated source: biological system reconstruction evidence based on homology evidence
annotated property: definition

biological system reconstruction evidence based on paralogy evidence
type: Class
subclass of: biological system reconstruction evidence based on homology evidence
creation date: 2015-12-18T16:24:08Z
definition: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species.
id: ECO:0005550
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species.
database_cross_reference: ECO:SN
database_cross_reference: PMID:15660128
annotated source: biological system reconstruction evidence based on paralogy evidence
annotated property: definition

biological system reconstruction evidence by experimental evidence
type: Class
subclass of: biological system reconstruction evidence
creation date: 2015-12-18T16:34:13Z
definition: A type of biological system reconstruction evidence that uses experimental evidence as support.
id: ECO:0005551
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence that uses experimental evidence as support.
database_cross_reference: ECO:SN
database_cross_reference: PMID:19450514
database_cross_reference: PMID:22232657
annotated source: biological system reconstruction evidence by experimental evidence
annotated property: definition

biological system reconstruction evidence by experimental evidence from mixed species
type: Class
subclass of: biological system reconstruction evidence by experimental evidence
creation date: 2015-12-18T16:35:13Z
definition: A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment.
id: ECO:0005552
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment.
database_cross_reference: ECO:SN
database_cross_reference: PMID:22232657
annotated source: biological system reconstruction evidence by experimental evidence from mixed species
annotated property: definition

biological system reconstruction evidence by experimental evidence from single species
type: Class
subclass of: biological system reconstruction evidence by experimental evidence
creation date: 2015-12-18T16:36:44Z
definition: A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species.
id: ECO:0005553
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species.
database_cross_reference: ECO:SN
annotated source: biological system reconstruction evidence by experimental evidence from single species
annotated property: definition

pairwise sequence alignment evidence
type: Class
subclass of: sequence alignment evidence
creation date: 2016-02-08T14:30:46Z
definition: A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).
id: ECO:0005554
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.ebi.ac.uk/Tools/psa/
annotated source: pairwise sequence alignment evidence
annotated property: definition

multiple sequence alignment evidence
type: Class
subclass of: sequence alignment evidence
creation date: 2016-02-08T14:31:24Z
definition: A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied.
id: ECO:0005555
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.ebi.ac.uk/Tools/msa/
annotated source: multiple sequence alignment evidence
annotated property: definition

multiple sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: multiple sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: multiple sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-08T14:59:18Z
definition: A type of multiple sequence alignment evidence that is used in a manual assertion.
id: ECO:0005556
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: sequence alignment evidence used in manual assertion
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of multiple sequence alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: multiple sequence alignment evidence used in manual assertion
annotated property: definition

multiple sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: multiple sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: multiple sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2016-02-08T15:00:50Z
definition: A type of multiple sequence alignment evidence that is used in an automatic assertion.
id: ECO:0005557
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: sequence alignment evidence used in automatic assertion
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of multiple sequence alignment evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN
annotated source: multiple sequence alignment evidence used in automatic assertion
annotated property: definition

motif discovery evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: motif discovery evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: motif discovery evidence
subclass of: multiple sequence alignment evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-08T15:07:47Z
definition: A type of motif discovery evidence that is used in a manual assertion.
id: ECO:0005558
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: multiple sequence alignment evidence used in manual assertion
annotated source: motif discovery evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of motif discovery evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: motif discovery evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: motif discovery evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

motif discovery evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: motif discovery evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: motif discovery evidence
subclass of: multiple sequence alignment evidence used in automatic assertion
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2016-02-08T15:08:19Z
definition: A type of motif discovery evidence that is used in an automatic assertion.
id: ECO:0005559
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: motif discovery evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

Axiom
is inferred: true
annotated target: multiple sequence alignment evidence used in automatic assertion
annotated source: motif discovery evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of motif discovery evidence that is used in an automatic assertion.
database_cross_reference: ECO:SN
annotated source: motif discovery evidence used in automatic assertion
annotated property: definition

pairwise sequence alignment evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: pairwise sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: sequence alignment evidence used in manual assertion
subclass of: pairwise sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-08T15:17:05Z
definition: A type of pairwise sequence alignment evidence that is used in a manual assertion.
id: ECO:0005560
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: sequence alignment evidence used in manual assertion
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of pairwise sequence alignment evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: pairwise sequence alignment evidence used in manual assertion
annotated property: definition

pairwise sequence alignment evidence used in automatic assertion
type: Class
equivalent class: Class
  intersection of: pairwise sequence alignment evidence
  intersection of: Restriction
    on property: used_in
    some values from: automatic assertion
subclass of: sequence alignment evidence used in automatic assertion
subclass of: pairwise sequence alignment evidence
subclass of: Restriction
  on property: used_in
  some values from: automatic assertion
creation date: 2016-02-08T15:17:52Z
definition: A type of pairwise sequence alignment evidence that is used in an automatic evidence.
id: ECO:0005561
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: sequence alignment evidence used in automatic assertion
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: subClassOf

Axiom
annotated target: A type of pairwise sequence alignment evidence that is used in an automatic evidence.
database_cross_reference: ECO:SN
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: definition

Axiom
is inferred: true
annotated source: pairwise sequence alignment evidence used in automatic assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: automatic assertion

in gel protein kinase assay evidence
type: Class
subclass of: in vitro assay evidence
creation date: 2016-02-09T12:05:29Z
id: ECO:0005562
has_obo_namespace: eco
created by: snadendla

macroscopic current trace evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-09T12:40:03Z
definition: A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording.
id: ECO:0005563
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording.
database_cross_reference: ECO:SN
database_cross_reference: PMID:17938230
database_cross_reference: url:http://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf
annotated source: macroscopic current trace evidence
annotated property: definition

current density evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-09T12:43:07Z
definition: A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane.
id: ECO:0005564
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/102/11/1298.full
database_cross_reference: url:http://www.bem.fi/book/04/04.htm
annotated source: current density evidence
annotated property: definition

single channel conductance evidence
type: Class
creation date: 2016-02-09T12:45:42Z
id: ECO:0005565
has_obo_namespace: eco
created by: snadendla
deprecated: true

sustained current evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-09T12:46:54Z
definition: A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels.
id: ECO:0005566
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html
annotated source: sustained current evidence
annotated property: definition

steady state activation curve evidence
type: Class
creation date: 2016-02-09T12:48:17Z
id: ECO:0005567
has_obo_namespace: eco
created by: snadendla
deprecated: true

steady state inactivation curve evidence
type: Class
creation date: 2016-02-09T12:50:45Z
id: ECO:0005568
has_obo_namespace: eco
created by: snadendla
deprecated: true

window current trace evidence
type: Class
creation date: 2016-02-09T12:57:16Z
id: ECO:0005569
has_obo_namespace: eco
created by: snadendla
deprecated: true

use dependence of inactivation evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-09T12:58:18Z
definition: A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course.
id: ECO:0005570
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/89/8/700.full
annotated source: use dependence of inactivation evidence
annotated property: definition

current clamp voltage recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T13:58:32Z
definition: A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell.
id: ECO:0005571
database_cross_reference: birnlex:2278
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html
annotated source: current clamp voltage recording evidence
annotated property: definition

current clamp voltage recording protocol
type: Axiom
annotated target: birnlex:2278
annotated source: current clamp voltage recording evidence
annotated property: database_cross_reference

whole-cell voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:06:40Z
definition: A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage.
id: ECO:0005572
database_cross_reference: birnlex:2281
has_obo_namespace: eco
created by: snadendla

whole-cell voltage clamp recording protocol
type: Axiom
annotated target: birnlex:2281
annotated source: whole-cell voltage clamp recording evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage.
database_cross_reference: ECO:SN
database_cross_reference: url:http://circres.ahajournals.org/content/72/1/91.full.pdf
annotated source: whole-cell voltage clamp recording evidence
annotated property: definition

cell-attached single-channel recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:07:39Z
definition: A type of electrophysiology assay evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction.
id: ECO:0005573
database_cross_reference: birnlex:2283
has_obo_namespace: eco
created by: snadendla

cell-attached single-channel recording protocol
type: Axiom
annotated target: birnlex:2283
annotated source: cell-attached single-channel recording evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of electrophysiology assay evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf
annotated source: cell-attached single-channel recording evidence
annotated property: definition

cell-detached inside-out single-channel recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:16:53Z
definition: A type of electrophysiology assay evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution.
id: ECO:0005574
database_cross_reference: birnlex:2286
has_obo_namespace: eco
created by: snadendla

cell-detached inside-out single-channel recording protocol
type: Axiom
annotated target: birnlex:2286
annotated source: cell-detached inside-out single-channel recording evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of electrophysiology assay evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.acnp.org/g4/GN401000005/CH005.html
annotated source: cell-detached inside-out single-channel recording evidence
annotated property: definition

reconstituted bilayer single-channel patch recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:17:46Z
definition: A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane.
id: ECO:0005575
database_cross_reference: birnlex:2287
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane.
database_cross_reference: ECO:SN
database_cross_reference: url:http://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf
annotated source: reconstituted bilayer single-channel patch recording evidence
annotated property: definition

reconstituted bilayer single-channel patch recording protocol
type: Axiom
annotated target: birnlex:2287
annotated source: reconstituted bilayer single-channel patch recording evidence
annotated property: database_cross_reference

voltage clamp current recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:18:36Z
definition: A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage.
id: ECO:0005576
database_cross_reference: birnlex:2279
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage.
database_cross_reference: ECO:SN
database_cross_reference: url:http://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf
database_cross_reference: url:http://www.acnp.org/g4/GN401000005/CH005.html
annotated source: voltage clamp current recording evidence
annotated property: definition

voltage clamp current recording protocol
type: Axiom
annotated target: birnlex:2279
annotated source: voltage clamp current recording evidence
annotated property: database_cross_reference

electroencephalography recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-02-12T14:19:21Z
definition: A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head.
id: ECO:0005577
database_cross_reference: birnlex:2293
has_obo_namespace: eco
created by: snadendla

electroencephalography recording protocol
type: Axiom
annotated target: birnlex:2293
annotated source: electroencephalography recording evidence
annotated property: database_cross_reference

Axiom
annotated target: A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.jove.com/science-education/5420/electro-encephalography-eeg
annotated source: electroencephalography recording evidence
annotated property: definition

super-resolution microscopy evidence
type: Class
subclass of: microscopy evidence
creation date: 2016-02-12T15:20:47Z
definition: A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures.
id: ECO:0005578
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures.
database_cross_reference: ECO:SN
database_cross_reference: url:http://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full
annotated source: super-resolution microscopy evidence
annotated property: definition

immunogold labelling evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunogold labelling evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunogold labelling evidence
subclass of: microscopy evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-12T15:38:03Z
definition: A type of immunogold labelling evidence that is used in a manual assertion.
id: ECO:0005579
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunogold labelling evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: immunogold labelling evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: immunogold labelling evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: microscopy evidence used in manual assertion
annotated source: immunogold labelling evidence used in manual assertion
annotated property: subClassOf

flow cytometry evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: flow cytometry evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: flow cytometry evidence
subclass of: direct assay evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-12T16:04:23Z
definition: A type of flow cytometry evidence that is used in a manual assertion.
id: ECO:0005580
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of flow cytometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: flow cytometry evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: flow cytometry evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: direct assay evidence used in manual assertion
annotated source: flow cytometry evidence used in manual assertion
annotated property: subClassOf

enzyme-linked immunoabsorbent assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: enzyme-linked immunoabsorbent assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: enzyme-linked immunoabsorbent assay evidence
subclass of: experimental evidence used in manual assertion
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-02-12T16:23:38Z
definition: A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion.
id: ECO:0005581
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: experimental evidence used in manual assertion
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: enzyme-linked immunoabsorbent assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

cell-detached outside-out single-channel recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-03-02T10:47:25Z
definition: A type of electrophysiology assay evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch).
id: ECO:0005582
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch).
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html
annotated source: cell-detached outside-out single-channel recording evidence
annotated property: definition

cut-open oocyte voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-03-02T11:29:32Z
definition: A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway.
id: ECO:0005583
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway.
database_cross_reference: ECO:SN
database_cross_reference: PMID:9711615
annotated source: cut-open oocyte voltage clamp recording evidence
annotated property: definition

macropatch voltage clamp recording evidence
type: Class
subclass of: electrophysiology assay evidence
creation date: 2016-03-02T11:33:48Z
definition: A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch).
id: ECO:0005584
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch).
database_cross_reference: ECO:SN
database_cross_reference: url:http://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf
annotated source: macropatch voltage clamp recording evidence
annotated property: definition

high throughput mass spectrometry evidence
type: Class
subclass of: mass spectrometry evidence
creation date: 2016-03-02T12:33:07Z
definition: A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies.
id: ECO:0005585
comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec. For HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies.
database_cross_reference: ECO:SN
database_cross_reference: PMID:20805795
annotated source: high throughput mass spectrometry evidence
annotated property: definition

high throughput mass spectrometry evidence used in manual assertion
type: Class
subclass of: high throughput mass spectrometry evidence
creation date: 2016-03-02T12:50:02Z
definition: A type of high throughput mass spectrometry evidence that is used in a manual assertion.
id: ECO:0005586
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of high throughput mass spectrometry evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: high throughput mass spectrometry evidence used in manual assertion
annotated property: definition

confocal microscopy evidence
type: Class
subclass of: microscopy evidence
creation date: 2016-03-17T12:29:45Z
definition: A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis.
id: ECO:0005587
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf
annotated source: confocal microscopy evidence
annotated property: definition

wide-field microscopy evidence
type: Class
subclass of: microscopy evidence
creation date: 2016-03-17T12:30:27Z
definition: A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times.
id: ECO:0005588
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times.
database_cross_reference: ECO:SN
database_cross_reference: url:http://jcb.rupress.org/content/172/1/9.full
database_cross_reference: url:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/
database_cross_reference: url:http://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html
annotated source: wide-field microscopy evidence
annotated property: definition

confocal microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: confocal microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: confocal microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-17T12:32:24Z
definition: A type confocal microscopy evidence that is used in a manual assertion.
id: ECO:0005589
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated source: confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type confocal microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: confocal microscopy evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: microscopy evidence used in manual assertion
annotated source: confocal microscopy evidence used in manual assertion
annotated property: subClassOf

wide-field microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: wide-field microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: wide-field microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-17T12:34:42Z
definition: A type of wide-field microscopy evidence that is used in a manual assertion.
id: ECO:0005590
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: microscopy evidence used in manual assertion
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of wide-field microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: wide-field microscopy evidence used in manual assertion
annotated property: definition

immunogold labelling electron microscopy assay evidence
type: Class
subclass of: immunogold labelling evidence
subclass of: electron microscopy evidence
creation date: 2016-03-17T15:43:00Z
definition: A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles.
id: ECO:0005591
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles.
database_cross_reference: ECO:SN
database_cross_reference: PMID:4110101
annotated source: immunogold labelling electron microscopy assay evidence
annotated property: definition

immunogold labelling electron microscopy assay evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunogold labelling electron microscopy assay evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunogold labelling evidence used in manual assertion
subclass of: immunogold labelling electron microscopy assay evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-17T16:39:37Z
definition: A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion.
id: ECO:0005592
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated target: immunogold labelling evidence used in manual assertion
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: immunogold labelling electron microscopy assay evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

immunodetection assay evidence
type: Class
subclass of: direct assay evidence
creation date: 2016-03-17T17:07:33Z
definition: A type of direct assay evidence that involves the use of antibodies to detect biomolecules.
id: ECO:0005593
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of direct assay evidence that involves the use of antibodies to detect biomolecules.
database_cross_reference: ECO:SN
annotated source: immunodetection assay evidence
annotated property: definition

immunoperoxidase immunolocalization evidence
type: Class
subclass of: immunolocalization evidence
creation date: 2016-03-17T17:11:34Z
definition: A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody.
id: ECO:0005594
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody.
database_cross_reference: ECO:SN
annotated source: immunoperoxidase immunolocalization evidence
annotated property: definition

immunoperoxidase immunolocalization evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunoperoxidase immunolocalization evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: microscopy evidence used in manual assertion
subclass of: immunoperoxidase immunolocalization evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-17T17:14:05Z
definition: A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion.
id: ECO:0005595
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: microscopy evidence used in manual assertion
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: subClassOf

Axiom
annotated target: A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: immunoperoxidase immunolocalization evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

immunoperoxidase immunolocalization electron microscopy evidence
type: Class
subclass of: electron microscopy evidence
subclass of: immunoperoxidase immunolocalization evidence
creation date: 2016-03-17T17:16:38Z
definition: A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody.
id: ECO:0005596
has_exact_synonym: Immunoperoxidase labelling electron microscopy
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody.
database_cross_reference: ECO:SN
annotated source: immunoperoxidase immunolocalization electron microscopy evidence
annotated property: definition

immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunoperoxidase immunolocalization electron microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: immunoperoxidase immunolocalization evidence used in manual assertion
subclass of: immunoperoxidase immunolocalization electron microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-17T17:23:56Z
definition: A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion.
id: ECO:0005597
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: definition

Axiom
is inferred: true
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
is inferred: true
annotated target: immunoperoxidase immunolocalization evidence used in manual assertion
annotated source: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion
annotated property: subClassOf

wide-field fluorescence microscopy evidence
type: Class
subclass of: wide-field microscopy evidence
creation date: 2016-03-18T11:53:56Z
definition: A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter.
id: ECO:0005598
comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.bristol.ac.uk/synaptic/research/techniques/widefield.html
annotated source: wide-field fluorescence microscopy evidence
annotated property: definition

immunofluorescence wide-field microscopy evidence
type: Class
subclass of: immunofluorescence evidence
subclass of: wide-field fluorescence microscopy evidence
creation date: 2016-03-18T11:55:40Z
definition: A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens.
id: ECO:0005599
has_obo_namespace: eco
created by: snadendla
has_exact_synonym: wide-field epifluorescence microscopy

Axiom
annotated target: A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens.
database_cross_reference: ECO:SN
database_cross_reference: url:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135
annotated source: immunofluorescence wide-field microscopy evidence
annotated property: definition

immunofluorescence confocal microscopy evidence
type: Class
subclass of: immunofluorescence evidence
subclass of: confocal microscopy evidence
creation date: 2016-03-18T12:48:42Z
definition: A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection.
id: ECO:0005600
comment: This allows visualization of sub cellular distribution of biomolecules of interest.
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection.
database_cross_reference: ECO:SN
database_cross_reference: url:http://www.physics.emory.edu/faculty/weeks/confocal/
database_cross_reference: url:https://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf
annotated source: immunofluorescence confocal microscopy evidence
annotated property: definition

immunofluorescence confocal microscopy evidence used in manual assertion
type: Class
equivalent class: Class
  intersection of: immunofluorescence confocal microscopy evidence
  intersection of: Restriction
    on property: used_in
    some values from: manual assertion
subclass of: confocal microscopy evidence used in manual assertion
subclass of: immunofluorescence confocal microscopy evidence
subclass of: Restriction
  on property: used_in
  some values from: manual assertion
creation date: 2016-03-18T12:52:04Z
definition: A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion.
id: ECO:0005601
has_obo_namespace: eco
created by: snadendla

Axiom
is inferred: true
annotated target: confocal microscopy evidence used in manual assertion
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: subClassOf

Axiom
is inferred: true
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: subClassOf
annotated target: Restriction
  on property: used_in
  some values from: manual assertion

Axiom
annotated target: A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion.
database_cross_reference: ECO:SN
annotated source: immunofluorescence confocal microscopy evidence used in manual assertion
annotated property: definition

protein expression level evidence based on western blot
type: Class
subclass of: protein expression level evidence
subclass of: western blot evidence
creation date: 2016-03-18T13:02:29Z
definition: A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique.
id: ECO:0005602
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique.
database_cross_reference: ECO:SN
annotated source: protein expression level evidence based on western blot
annotated property: definition

protein mass spectrometry evidence
type: Class
subclass of: protein assay evidence
subclass of: mass spectrometry evidence
creation date: 2016-03-18T13:11:37Z
definition: A type of mass spectrometry evidence where one or more protein analyses are performed.
id: ECO:0005603
has_obo_namespace: eco
created by: snadendla

Axiom
annotated target: A type of mass spectrometry evidence where one or more protein analyses are performed.
database_cross_reference: ECO:SN
annotated source: protein mass spectrometry evidence
annotated property: definition

protein-binding microarray evidence
type: Class
subclass of: oligonucleotide microarray evidence
subclass of: nucleic acid binding evidence
creation date: 2015-06-05T14:36:00Z
definition: A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites.
id: ID:0001601
has_exact_synonym: PBM evidence
has_obo_namespace: eco
created by: swolfish

Axiom
annotated target: A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites.
database_cross_reference: PMID:22146299
annotated property: definition
annotated source: protein-binding microarray evidence

in vivo cell staining evidence
type: Class
subclass of: in vivo assay evidence
creation date: 2015-06-08T11:51:37Z
definition: A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death.
id: ID:0001603
has_obo_namespace: eco
created by: swolfish
has_related_synonym: vital staining evidence

Axiom
annotated target: A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death.
database_cross_reference: GOC:MAH
annotated property: definition
annotated source: in vivo cell staining evidence

Axiom
database_cross_reference: GOC:MAH
annotated target: vital staining evidence
annotated source: in vivo cell staining evidence
annotated property: has_related_synonym

computational image-based phenotypic evidence
type: Class
creation date: 2015-06-09T16:13:20Z
definition: A type of phenotypic evidence based on unsupervised computational examination of a visual mutant phenotype such as abnormalities in organism morphology.
id: ID:0001706
has_obo_namespace: eco
created by: kvillanova
deprecated: true

Axiom
annotated target: A type of phenotypic evidence based on unsupervised computational examination of a visual mutant phenotype such as abnormalities in organism morphology.
database_cross_reference: ECO:KIM
database_cross_reference: PMID:25373780
annotated property: definition
annotated source: computational image-based phenotypic evidence