format-version: 1.2 data-version: releases/2022-08-05 date: 05:08:2022 15:34 subsetdef: go_groupings "Grouping classes used by GO" subsetdef: valid_with_biological_process "GO biological process terms should be used in the GO with/from field" subsetdef: valid_with_cellular_component "GO cellular component terms should be used in the GO with/from field" subsetdef: valid_with_chemical_entity "Chemical entity IDs should be used in the GO with/from field" subsetdef: valid_with_gene "Gene IDs should be used in the GO with/from field" subsetdef: valid_with_molecular_function "GO molecular function terms should be used in the GO with/from field" subsetdef: valid_with_protein "Protein IDs should be used in the GO with/from field" subsetdef: valid_with_protein_complex "Protein complex IDs should be used in the GO with/from field" subsetdef: valid_with_transcript "Transcript IDs should be used in the GO with/from field" default-namespace: eco remark: ECO (https://github.com/evidenceontology/evidenceontology) is released into the public domain under CC0 1.0 Universal (CC0 1.0). Anyone is free to copy, modify, or distribute the work, even for commercial purposes, without asking permission. Please see the Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/) for an easy-to-read description of CC0 1.0 or the full legal code (https://creativecommons.org/publicdomain/zero/1.0/legalcode) for more detailed information. To get a sense of why ECO is CC0 as opposed to licensed under CC-BY, please read this thoughtful discussion (https://github.com/OBOFoundry/OBOFoundry.github.io/issues/285) on the OBO Foundry GitHub site. ontology: eco property_value: http://purl.org/dc/elements/1.1/description "The Evidence & Conclusion Ontology (ECO) describes types of scientific evidence within the biological research domain that arise from laboratory experiments, computational methods, literature curation, or other means." xsd:string property_value: http://purl.org/dc/elements/1.1/title "Evidence & Conclusion Ontology (ECO)" xsd:string property_value: http://purl.org/dc/terms/license https://creativecommons.org/publicdomain/zero/1.0/ [Term] id: ECO:0000000 name: evidence def: "A type of information that is used to support an assertion." [ECO:MCC] synonym: "evidence code" RELATED [] synonym: "evidence_code" RELATED [] disjoint_from: ECO:0000217 ! assertion method [Term] id: ECO:0000001 name: inference from background scientific knowledge def: "A type of curator inference where conclusions are drawn based on the background scientific knowledge of the curator." [ECO:SN] is_a: ECO:0000205 ! curator inference [Term] id: ECO:0000002 name: direct assay evidence alt_id: ECO:0005006 def: "A type of experimental evidence resulting from the direct measurement of some aspect of a biological feature." [ECO:SN] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000003 name: reconstitution assay evidence def: "A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state." [ECO:KAV, PMID:26029343] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000004 name: cell fractionation evidence def: "A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient." [ECO:KIM, TAIR:TED] comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology. synonym: "cell fractionation" RELATED [] is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000005 name: enzymatic activity assay evidence alt_id: ECO:0005001 def: "A type of protein assay evidence where the catalytic activity of an enzyme is determined." [url:http\://www.sciencedirect.com/science/article/pii/S2213020914000068] synonym: "enzyme assay evidence" EXACT [] synonym: "enzyme assays" RELATED [] xref: MI:0415 "enzymatic study" is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000006 name: experimental evidence alt_id: ECO:0005023 def: "A type of evidence that is the output of a scientific procedure performed to make a discovery, test a hypothesis, or demonstrate a known fact." [OED:experiment] subset: go_groupings is_a: ECO:0000000 ! evidence relationship: OBI:0000295 OBI:0000338 relationship: OBI:0000312 OBI:0000070 [Term] id: ECO:0000007 name: immunofluorescence evidence def: "A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell." [ECO:MCC, TAIR:TED] synonym: "immunofluorescence" RELATED [] is_a: ECO:0005517 ! protein detection assay evidence [Term] id: ECO:0000008 name: expression pattern evidence def: "A type of experimental evidence that is based on characterization of gene expression." [ECO:MCC, GO:IEP] comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process. is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000112 "The 10 previously identified GlnR-regulated genes were all confirmed to be under GlnR control during nitrogen stress (i.e. differential expression in the wild type compared to the DeltaglnR mutant), but in addition a total of 392 genes were significantly up-regulated and 291 significantly down regulated (Additional file 1: Table S1). This indicates that GlnR mediates (directly or indirectly) the expression of over 680 genes." xsd:string {xref="PMID:23642041"} [Term] id: ECO:0000009 name: transcript expression evidence alt_id: ECO:0000048 def: "A type of expression pattern evidence where abundance of a transcript is analyzed." [ECO:RCT] synonym: "transcript expression level evidence" EXACT [] synonym: "transcriptomics evidence" EXACT [] is_a: ECO:0000008 ! expression pattern evidence [Term] id: ECO:0000010 name: protein expression evidence alt_id: ECO:0000046 def: "A type of expression pattern evidence resulting from protein abundance quantification techniques." [NBK:22011, PMC:PMC4029002, url:http\://www.informatics.jax.org/glossary/gain-of-function, url:https\://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-protein-expression-systems.html] synonym: "protein expression level evidence" EXACT [] is_a: ECO:0000008 ! expression pattern evidence property_value: IAO:0000112 "In the BL21(DE3)(pJS429) cells, the levels of ClpP2s and VapC10 remained unchanged over a 2-hour period of translation arrest, but the VapB10 level showed to be decreased with a half-life (Figure 7C) similar to that observed in the strain BL21(DE3)(pJS883) (Figure 7B). These indicate that ClpXP2s could degrade VapB10 regardless of the presence or absence of VapC10." xsd:string {xref="PMID:24260461"} [Term] id: ECO:0000011 name: genetic interaction evidence def: "A type of experimental phenotypic evidence resulting from the effect that a given gene has on another gene or genes, and the products." [ECO:RCT, PMID:11822023] xref: TAIR:TED is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000012 name: functional complementation evidence def: "A type of genetic interaction evidence where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] synonym: "functional complementation" RELATED [] is_a: ECO:0000011 ! genetic interaction evidence property_value: IAO:0000112 "In addition, complementation of the ompR mutation in strain AR6 with plasmid pBR3 resulted in an increase in beta-galactosidase activity (1303 +- 80 Miller units), indicating that the His-tagged OmpR protein, expressed from the gene introduced in trans, was able to positively regulate flhDC expression." xsd:string {xref="PMID:20830609"} property_value: IAO:0000112 "Trans-complementation of fimR on pDL276 (pHR6) in strain DeltafimR harboring pfim(445 b)-cat restored wild-type pfim expression (Figure S1). Taken together, pfim is negatively regulated by FimR." xsd:string {xref="PMID:23823757"} [Term] id: ECO:0000013 name: transgenic rescue experiment evidence def: "A type of functional complementation evidence resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.mdpi.com/1420-3049/19/9/13932/pdf, url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000015 name: mutant phenotype evidence def: "A type of experimental phenotypic evidence in which an observable phenotypic difference results from a change or mutation in DNA." [GO:IMP] comment: Note that mutations need not be negative. Changes to DNA sequence (mutations) may be detrimental, have no impact, or be beneficial. comment: The allele that encodes the phenotype most common in a particular natural population is referred to as the wild type allele, while any other form of that allele is known as the mutant form. is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000112 "Indeed, the GroEL protein appears to be more abundant in the LM3 wild type strain compared to the LM3-2 mutant strain, suggesting the involvement of the CcpA protein in the positive regulation of its expression." xsd:string {xref="PMID:17129387"} [Term] id: ECO:0000016 name: loss-of-function mutant phenotype evidence def: "A type of mutant phenotype evidence where a phenotype is associated with altered gene product which lacks the molecular function of the wild-type gene." [SO:0002054] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000017 name: ectopic expression evidence def: "A type of experimental phenotypic evidence where a transgenic strain carrying the construct of a promoter cDNA fusion in which a gene of interest is driven by a defined promoter or enhancer is ectopically expressed in the defined pattern to characterize potential cellular properties and functions of a protein of interest." [PMID:10948520, PMID:19301619] synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000018 name: anti-sense experiment evidence def: "A type of mutant phenotype evidence where a phenotype is observed while expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." [ECO:SN] synonym: "anti-sense experiments" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000019 name: RNAi evidence def: "A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed." [ECO:MCC] synonym: "RNAi experiment" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000020 name: protein inhibition evidence def: "A type of direct assay evidence based on the inhibition of the molecular function of a protein." [ECO:MCC] synonym: "specific protein inhibition evidence" NARROW [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000021 name: physical interaction evidence alt_id: ECO:0005025 def: "A type of experimental evidence that is based on characterization of an interaction between a gene product and another molecule." [ECO:SN] comment: Molecules interacted with might include protein, nucleic acid, ion, or complex. xref: MI:0045 "experimental interaction detection" is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000022 name: co-purification evidence def: "A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] synonym: "co-purification" RELATED [] xref: MI:0025 "copurification" is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000023 name: affinity evidence def: "A type of physical interaction evidence that depends on the strength of the interaction between two entities." [ECO:MCC, MI:0400] synonym: "ligand binding evidence" NARROW [] xref: MI:0400 "affinity technology" is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000024 name: protein binding evidence def: "A type of affinity evidence resulting from the binding of a molecule to a protein or protein complex." [GO:0005515, url:https\://en.wikipedia.org/wiki/Mutation] is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000025 name: bait-prey hybrid interaction evidence def: "A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, MI:0090] comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode. synonym: "bait-prey protein pull-down evidence" EXACT [] xref: MI:0090 "protein complementation assay" is_a: ECO:0006351 ! bait-prey interaction evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string [Term] id: ECO:0000026 name: nucleic acid hybridization evidence def: "A type of experimental genomic evidence resulting from a process in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences." [OBI:0302903] comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. is_obsolete: true [Term] id: ECO:0000027 name: structural similarity evidence def: "A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes." [ECO:MCC, TAIR:TED] comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field. is_a: ECO:0000057 ! phenotypic similarity evidence property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} [Term] id: ECO:0000028 name: motif similarity evidence def: "A type of match to sequence model evidence that is based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." [ECO:SN] synonym: "recognized domains" RELATED [] is_a: ECO:0000202 ! match to sequence model evidence property_value: IAO:0000112 "A CRP box-like sequence was found in the promoter-proximate region of sycO-ypkA-yopJ [4], indicating the direct association of CRP with the sycO-ypkA-yopJ promoter region." xsd:string {xref="PMID:19703315"} property_value: IAO:0000112 "To identify further CopR target genes, the binding motif TGAAGATTTnnTGAAGATTT was used to search for similar sequences in the whole C. glutamicum genome using the ERGO (TM) bioinformatics suite (Integrated Genomics, Illinois, USA) allowing four mutations, no deletions and no insertions. 46 hits were found, but only in six cases the putative CopR binding site was located in intergenic regions up to 200 bp upstream of the start codon of the neighbouring gene (cg1336, cg2976, cg3187, cg3337, cg3357 and cg0414)." xsd:string {xref="PMID:21799779"} [Term] id: ECO:0000029 name: match to InterPro member signature evidence def: "A type of match to sequence model evidence resulting from a positive match of a protein, or set of proteins to a predictive model (signature) in the InterPro database." [PMC:PMC2686546, url:http\://www.ncbi.nlm.nih.gov/mesh?term=Nucleic+Acid+Hybridization] is_a: ECO:0000202 ! match to sequence model evidence [Term] id: ECO:0000030 name: BLAST evidence used in manual assertion def: "A type of BLAST evidence that is used in a manual assertion." [ECO:MCC] synonym: "curated BLAST analysis" RELATED [] is_a: ECO:0000206 ! BLAST evidence is_a: ECO:0005560 {is_inferred="true"} ! pairwise sequence alignment evidence used in manual assertion intersection_of: ECO:0000206 ! BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string [Term] id: ECO:0000031 name: protein BLAST evidence used in manual assertion def: "A type of protein BLAST evidence that is used in a manual assertion." [ECO:SN] synonym: "curated protein BLAST analysis" RELATED [] xref: GO_REF:0000012 "Pairwise alignment (TIGR)" xref: GO_REF:0000027 "BLAST search criteria for ISS assignment in PAMGO_GAT" is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion is_a: ECO:0000208 ! protein BLAST evidence intersection_of: ECO:0000208 ! protein BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string [Term] id: ECO:0000032 name: nucleotide BLAST evidence used in manual assertion def: "A type of nucleotide BLAST evidence that is used in a manual assertion." [ECO:SN] synonym: "curated nucleic acid BLAST analysis" RELATED [] is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion is_a: ECO:0000207 ! nucleotide BLAST evidence intersection_of: ECO:0000207 ! nucleotide BLAST evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string [Term] id: ECO:0000033 name: author statement supported by traceable reference def: "A type of author statement in which the author makes a statement that is not supported by information in that particular publication, but rather can be traced to a reference cited by that publication." [ECO:RCT, GO:TAS] synonym: "traceable author statement" RELATED [] is_a: ECO:0000204 ! author statement disjoint_from: ECO:0000034 ! author statement without traceable support [Term] id: ECO:0000034 name: author statement without traceable support def: "A type of author statement that is not associated with results presented or a cited reference." [ECO:SN] synonym: "non-traceable author statement" RELATED [] is_a: ECO:0000204 ! author statement [Term] id: ECO:0000035 name: no evidence data found def: "A type of curator inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources." [ECO:SN] comment: An assertion of "no evidence data found" carries the assumption that a more-or-less exhaustive search has been conducted. is_a: ECO:0000205 ! curator inference [Term] id: ECO:0000037 name: not_recorded comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations. is_obsolete: true [Term] id: ECO:0000038 name: transient rescue experiment evidence def: "A type of functional complementation evidence resulting from the introduction of nucleic acids, which are not permanently incorporated into the genome, to temporarily prevent, or \"rescue\" an organism from a condition." [] is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000039 name: protein assay evidence alt_id: ECO:0005022 def: "A type of direct assay evidence resulting from determining the presence, abundance, structure, function, or activity of proteins." [PMID:18429326] is_obsolete: true [Term] id: ECO:0000040 name: immunological assay evidence alt_id: ECO:0005018 def: "A type of affinity evidence resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362, url:http\://www.ncbi.nlm.nih.gov/books/NBK21589/\,http\://cores.ucsf.edu/protein-assay.html] is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000041 name: similarity evidence def: "A type of evidence resulting from comparing likeness of distinct biological entities." [ECO:SN] synonym: "inferred from similarity" RELATED [] synonym: "IS" EXACT [] is_a: ECO:0000000 ! evidence [Term] id: ECO:0000042 name: gain-of-function mutant phenotypic evidence def: "A type of mutant phenotype evidence resulting from an altered gene product which possesses a new molecular function or a new pattern of gene expression." [SO:0002053, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614608/] synonym: "gain-of-function mutant phenotype evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000044 name: sequence similarity evidence def: "A type of similarity based on biomolecular sequence." [ECO:MCC, TAIR:TED] comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes. is_a: ECO:0000041 ! similarity evidence is_a: ECO:0007672 ! computational evidence [Term] id: ECO:0000045 name: spatial pattern of protein expression evidence def: "A type of protein expression evidence that accounts for the position of RNA translation to a protein product ranging in scale from differing tissues to regions of an anatomical structure." [] is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000047 name: spatial pattern of transcript expression evidence def: "A type of transcript expression evidence that accounts for the position of transcription ranging in scale from the position of genes on the chromosome to differing tissues to regions of an anatomical structure." [] is_a: ECO:0000009 ! transcript expression evidence [Term] id: ECO:0000049 name: reporter gene assay evidence def: "A type of expression pattern evidence that is based on the expression pattern of a reporter gene." [TAIR:TED] synonym: "expression of a reporter gene" RELATED [] is_a: ECO:0000008 ! expression pattern evidence [Term] id: ECO:0000050 name: voucher specimen phenotypic analysis evidence def: "A type of experimental phenotypic evidence based on an authors phenotypic description of a species (or higher-level group), which explicitly references an observation made of a voucher specimen (a specimen with a permanent museum catalog)." [TAIR:TED] synonym: "IVS" EXACT [] synonym: "voucher specimen analysis evidence" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000051 name: genetic similarity evidence def: "A type of similarity based on genotype without respect to expression." [ECO:MCC, PhenoScape:IGTS] comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology. synonym: "IGTS" EXACT [] synonym: "inferred from genetic similarity" RELATED [] is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000052 name: suppressor/enhancer interaction phenotypic evidence def: "A type of genetic interaction evidence resulting from a second mutation that either suppresses or enhances the phenotype expressed from an original mutation." [url:http\://biorxiv.org/content/early/2015/10/03/021592, url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" RELATED [] synonym: "suppressor/enhancer interaction evidence" EXACT [] xref: TAIR:TED is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000053 name: automatically integrated combinatorial evidence used in automatic assertion alt_id: ECO:0000246 def: "A type of automatically integrated combinatorial evidence that is used in an automatic assertion." [ECO:MCC, ECO:RCT] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. xref: TAIR:TED is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion is_a: ECO:0007673 {is_inferred="true"} ! automatically integrated combinatorial evidence intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string [Term] id: ECO:0000054 name: double mutant phenotypic evidence def: "A type of mutant phenotype evidence resulting from an experiment typically constructed to determine if two different genes have an observable genetic interaction (functional connection) as the result of a mutation occurring in the alleles of the two genes of interest." [ECO:RCT, PMID:18305163] synonym: "double mutant analysis" RELATED [] synonym: "double mutant phenotype evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000112 "In the hns slyA double mutant, hlyE expression was twofold greater than in the parent, and slightly higher than the hns single mutant, suggesting that slyA has a small negative effect on hlyE expressionin the absence of H-NS." xsd:string {xref="PMID:17892462"} [Term] id: ECO:0000055 name: array experiment evidence comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000097, ECO:0000062, ECO:0000104, etc.) is_obsolete: true [Term] id: ECO:0000056 name: epistatic interaction phenotypic evidence def: "A type of genetic interaction evidence resulting from the suppression of one allelic effect by an allele at another genetic locus." [PMID:18852697] comment: Epistasis' can be used in different contexts in different areas of genetics. It is sometimes used to mean 'genetic interaction', whereas other times it may be specific to mutations that block the effects of other mutations. synonym: "epistatic interaction evidence" EXACT [] synonym: "epistatic interactions" RELATED [] is_a: ECO:0000011 ! genetic interaction evidence [Term] id: ECO:0000057 name: phenotypic similarity evidence def: "A type of similarity based on the expression of a genotype in an environment." [ECO:MCC, PhenoScape:IPTS] comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms. synonym: "inferred from phenotypic similarity" RELATED [] synonym: "IPTS" EXACT [] synonym: "phenotype similarity evidence" EXACT [] is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000058 name: expression microarray evidence alt_id: ECO:0000356 def: "A type of transcript expression evidence resulting from simultaneous profiling of the expression levels of thousands of genes in a single experiment allowing analysis of genes and their networks." [url:http\://www.illumina.com/techniques/microarrays/gene-expression-arrays.html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435252/] synonym: "differential gene expression evidence from microarray experiment" NARROW [] is_a: ECO:0000009 ! transcript expression evidence property_value: IAO:0000112 "S. lividans AdpA directly regulates at least the six AdpA-dependent genes listed above and identified by microarrays and qRT-PCR analysis." xsd:string {xref="PMID:24694298"} property_value: IAO:0000112 "To identify the regulon of the response regulator CopR, the transcriptome of the DeltacopRS deletion mutant was compared to that of the wild type using DNA microarrays. For cells grown in standard CGXII medium (1.25 microM CuSO4), no significant gene expression differences were observed, indicating that the CopRS two-component system is not active under this condition (data not shown)." xsd:string {xref="PMID:21799779"} [Term] id: ECO:0000059 name: experimental phenotypic evidence alt_id: ECO:0000014 alt_id: ECO:0005017 def: "A type of experimental evidence that is based on an observable characteristic trait, which is the result of the expression of an organisms genotype in an environment." [ECO:SN] comment: Observable characteristic traits can be morphology, development, behavior, biochemical or physiological properties, etc. synonym: "inferred from phenotype" RELATED [] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000060 name: positional similarity evidence def: "A type of phenotypic similarity evidence based on the similarity of stucture locations or arrangements." [TAIR:TED] synonym: "IPS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000061 name: quantitative trait analysis evidence def: "A type of phenotypic evidence that is based on a gene product that is associated with a quantitative trait locus, but has not been cloned." [TAIR:TED] synonym: "QTL analysis evidence" EXACT [] synonym: "quantitative trait analysis" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000062 name: cRNA to DNA expression microarray evidence def: "A type of expression microarray evidence where expression level is quantified by sample biotin-labeled cRNA (transcribed from an unknown RNA sample) hybridized to DNA oligonuclotides immoblized on a solid surface." [url:https\://link.springer.com/chapter/10.1007/978-94-017-9716-0_30] synonym: "genomic microarray evidence" EXACT [] is_a: ECO:0000058 ! expression microarray evidence [Term] id: ECO:0000063 name: compositional similarity evidence def: "A type of phenotypic similarity evidence based on the similarity of the histological makeup of structures." [TAIR:TED] synonym: "ICS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000064 name: functional complementation in heterologous system evidence def: "A type of functional complementation evidence that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] synonym: "functional complementation in heterologous system" RELATED [] is_a: ECO:0000012 ! functional complementation evidence [Term] id: ECO:0000066 name: yeast one-hybrid evidence def: "A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC, MI:0432, TAIR:TED] comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED). synonym: "yeast one-hybrid assay" RELATED [] xref: MI:0432 "one hybrid" is_a: ECO:0000025 ! bait-prey hybrid interaction evidence [Term] id: ECO:0000067 name: developmental similarity evidence def: "A type of phenotypic similarity evidence based on the similarity of embryological or post-embryonic orgin of structures." [TAIR:TED] synonym: "IDS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000068 name: yeast 2-hybrid evidence def: "A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain." [PMID:12734586] synonym: "yeast two-hybrid assay" RELATED [] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence [Term] id: ECO:0000069 name: differential methylation hybridization evidence alt_id: ECO:0000065 def: "A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment." [PMID:18987809] comment: Color is indicative of methylation status - be it hypo- (pseudo-green), hyper- (pseudo-red), or equal methylation (pseudo-yellow). synonym: "CpG island microarray evidence" EXACT [] is_a: ECO:0001065 ! in vitro methylation assay evidence [Term] id: ECO:0000070 name: co-immunoprecipitation evidence def: "A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification." [ECO:MCC] comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column. synonym: "co-immunoprecipitation" RELATED [] xref: MI:0019 "coimmunoprecipitation" is_a: ECO:0000085 ! immunoprecipitation evidence [Term] id: ECO:0000071 name: morphological similarity evidence def: "A type of phenotypic similarity evidence based on the similarity of shape, structure, or configuration in structures." [TAIR:TED] synonym: "IMS" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000072 name: Sos-recruitment assay evidence is_obsolete: true [Term] id: ECO:0000073 name: experimental genomic evidence def: "A type of experimental evidence that is based on the characterization of an attribute of the genome underlying a gene product." [ECO:MCC] comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. synonym: "inferred from genomic analysis" EXACT [] is_obsolete: true [Term] id: ECO:0000074 name: split-ubiquitin functional complementation evidence def: "A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] comment: The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquitin (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough to reconstitute.TAIR:TED synonym: "split-ubiquitin assay" RELATED [] xref: MI:0112 "ubiquitin reconstruction" is_a: ECO:0006256 ! protein fragment functional complementation evidence [Term] id: ECO:0000075 name: gene expression similarity evidence def: "A type of phenotypic similarity evidence that is based on the categorization of genes by the similarity of expression profiles." [PMID:19958477] synonym: "IGES" EXACT [] is_a: ECO:0000057 ! phenotypic similarity evidence [Term] id: ECO:0000076 name: far-Western blotting evidence def: "A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] comment: The interacting protein is referenced in the evidence_with column. synonym: "far-Western analysis" RELATED [] xref: MI:0047 is_a: ECO:0000021 ! physical interaction evidence [Term] id: ECO:0000077 name: methylation-specific polymerase chain reaction evidence def: "A type of in vitro methylation assay evidence resulting from initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA." [PMC:PMC38513, PMID:8790415] comment: The product is the amplification of existing sequences - i.e. more DNA that can be further processed e.g. sequenced, DNA fingerprinting. synonym: "methylation-specific PCR evidence" EXACT [] synonym: "MS-PCR" EXACT [] synonym: "MSP" EXACT [] is_a: ECO:0001065 ! in vitro methylation assay evidence [Term] id: ECO:0000078 name: southern hybridization evidence def: "A type of DNA detection assay evidence that is based on detection of a specific DNA sequence by hybridization of labeled probes to any immobilized DNA fragment with sequence similarity." [PMID:18432697] comment: The DNA fragments are prepared through gel electrophoresis then transferred to a filter membrane. synonym: "Southern blot" EXACT [] synonym: "Southern blotting" RELATED [] is_a: ECO:0005519 ! DNA detection assay evidence [Term] id: ECO:0000079 name: affinity chromatography evidence def: "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bound compound." [ECO:MCC, TAIR:TED] comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand. synonym: "affinity chromatography" RELATED [] xref: MI:0004 "affinity chromatography technology" is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000080 name: phylogenetic evidence def: "A type of similarity that indicates common ancestry." [ECO:MCC, PhenoScape:IP] synonym: "IP" EXACT [PhenoScape:IP] is_a: ECO:0000041 ! similarity evidence property_value: IAO:0000112 "Comparative phylogenomics along with MLST (multilocus sequence typing) and whole genome sequecing has shown that ribotype 078 lineage is different than other C. difficile lineages [22]." xsd:string {xref="PMID:24713082"} [Term] id: ECO:0000081 name: targeting sequence prediction evidence def: "A type of motif similarity evidence that is based on detection of a targeting sequence in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] synonym: "targeting sequence prediction" RELATED [] is_a: ECO:0000028 ! motif similarity evidence [Term] id: ECO:0000082 name: polymerase chain reaction evidence def: "A type of experimental genomic evidence where DNA polymerase is used to synthesize new strand of DNA complementary to the offered template strand." [OBI:0000415] comment: This term has been made obsolete because PCR is not evidence. The children have been appropriately placed under other parents. synonym: "PCR evidence" EXACT [] is_obsolete: true [Term] id: ECO:0000083 name: transmembrane domain prediction evidence def: "A type of motif similarity evidence that is based on detection of one, or more, transmembrane domains in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] synonym: "transmembrane domain prediction" RELATED [] is_a: ECO:0000028 ! motif similarity evidence [Term] id: ECO:0000084 name: gene neighbors evidence def: "A type of genomic context evidence in which a gene product's identity is supported on the basis of the identity of neighboring genes." [ECO:MCC, GOC:MG] comment: Genomic cluster analyses include synteny and operon structure. synonym: "ICL" RELATED [] synonym: "inferred from genome cluster" EXACT [] xref: GO_REF:0000025 "Operon structure as IGC evidence" is_a: ECO:0000177 ! genomic context evidence created_by: mgiglio creation_date: 2009-03-20T11:55:18Z [Term] id: ECO:0000085 name: immunoprecipitation evidence def: "A type of protein binding evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation." [ECO:MCC, ECO:SW, TAIR:TED] comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding. synonym: "immunoprecipitation" RELATED [] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000086 name: amplification of intermethylated sites evidence def: "A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell." [PMID:18987810, url:https\://www.ncbi.nlm.nih.gov/probe/docs/techpcr/] comment: The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events. synonym: "AIMS" EXACT [] is_a: ECO:0001065 ! in vitro methylation assay evidence [Term] id: ECO:0000087 name: immunolocalization evidence def: "A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues." [ECO:MCC, ECO:SN] synonym: "immunolocalization" RELATED [] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0005593 ! immunodetection assay evidence [Term] id: ECO:0000088 name: biological system reconstruction evidence def: "A type of curator inference that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system." [ECO:MCC, GOC:mg] comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak. synonym: "inferred from system reconstruction" RELATED [] synonym: "ISR" RELATED [] is_a: ECO:0000205 ! curator inference created_by: mgiglio creation_date: 2009-03-20T12:00:17Z [Term] id: ECO:0000089 name: restriction landmark genomic scanning evidence alt_id: ECO:0001125 def: "A type of DNA detection assay evidence resulting from the use of restriction enzymes for direct end-labeling of DNA (creating landmarks), followed by high-resolution two-dimensional electrophoresis to visualize the landmarks." [PMID:8388788] synonym: "restriction landmark genome scanning evidence" EXACT [] synonym: "RLGS evidence" EXACT [] is_a: ECO:0005519 ! DNA detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string [Term] id: ECO:0000090 name: immunogold labelling evidence def: "A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy." [ECO:MCC] synonym: "immunogold labelling" RELATED [] is_a: ECO:0000087 ! immunolocalization evidence [Term] id: ECO:0000092 name: epitope-tagged protein immunolocalization evidence def: "A type of immunolocalization evidence in which recombinant proteins fused with epitopes are recognized by antibodies." [TAIR:TED] synonym: "immunolocalization of epitope-tagged protein" RELATED [] is_a: ECO:0000087 ! immunolocalization evidence [Term] id: ECO:0000093 name: array-based sequence capture evidence def: "A type of DNA detection assay evidence evidence where gene sequence or variation is determined or detected by fluorescently-labeled DNA fragments hybridized to sequence-specific oligonucleotides immobilized on an array." [PMID:21049075] synonym: "DNA microarray" BROAD [] synonym: "oligonucleotide microarray evidence" BROAD [] synonym: "single nucleotide polymorphism array evidence" NARROW [] synonym: "SNP array evidence" NARROW [] is_a: ECO:0005519 ! DNA detection assay evidence [Term] id: ECO:0000094 name: biological assay evidence comment: The children of 'biological assay evidence' have been moved under 'direct assay evidence', and this term has been deprecated with no children left. is_obsolete: true [Term] id: ECO:0000095 name: cell growth regulation assay evidence def: "A type of cell growth assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect." [ECO:SW, PomBase:MAH] comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter. synonym: "growth curve analysis" RELATED [] is_a: ECO:0001563 ! cell growth assay evidence created_by: mchibucos creation_date: 2014-10-15T00:58:50Z [Term] id: ECO:0000096 name: electrophoretic mobility shift assay evidence def: "A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid." [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED] comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid. synonym: "electrophoretic mobility shift assay" RELATED [] synonym: "EMSA: electrophoretic mobility shift assay" EXACT [] synonym: "Gel retardation assay" RELATED [PMID:17703195] xref: MI:0413 "electrophoretic mobility shift assay" is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000112 "Each of the promoter-proximal regions of qrr2 - 4 was subjected to EMSA with the purified His-OpaR protein (Fig. 6b). The results showed that His-OpaR was able to bind to each of the three DNA fragments tested in a dose-dependent manner in vitro." xsd:string {xref="PMID:22506036"} property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000097 name: cDNA to DNA expression microarray evidence alt_id: ECO:0001041 alt_id: ECO:0005524 def: "A type of expression microarray evidence where expression level is quantified by fluroescently-labeled cDNA (reverse transcribed from an unknown RNA sample) hybridized to DNA immobilized on a solid surface." [ECO:RCT] synonym: "DNA microarray" RELATED [] synonym: "RNA microarray" RELATED [] is_a: ECO:0000058 ! expression microarray evidence property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000098 name: obsolete in situ hybridization evidence def: "A type of nucleic acid hybridization evidence based on localization of a specific segment of DNA or RNA by the application of a complementary strand of nucleic acid to which a reporter molecule is attached." [url:https\://www.ncbi.nlm.nih.gov/probe/docs/techish/] comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. synonym: "in situ hybridization" RELATED [] is_obsolete: true [Term] id: ECO:0000100 name: fractionation evidence def: "A type of direct assay evidence resulting from the separation of various cell components while preserving their individual functions." [NBK:26936, url:https\://www.ncbi.nlm.nih.gov/books/NBK26936/] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000101 name: Affymetrix GeneChip evidence def: "A type of cRNA to DNA expression microarray evidence resulting from the use of a proprietary Affymetrix GeneChip System for analyzing complex genetic information." [ERO:0001265] synonym: "Affymetrix array experiment evidence" EXACT [] is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence [Term] id: ECO:0000102 name: co-fractionation evidence def: "A type of fractionation evidence resulting from a protein fractionated with other compounds, factors, or macromolecules." [TAIR:TED] synonym: "co-fractionation" RELATED [] is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000104 name: DNA to cDNA expression microarray evidence def: "A type of expression microarray evidence where expression levels are quantified by hybridization of fluorescently-labeled DNA to cDNA (reverse transcribed from an unknown RNA sample) immobilized on a solid surface." [ECO:RCT, PMID:15329382] comment: REM is a 'reverse-format' microarray, where the unknown sample is immoblized to a surface and known DNA is hybridized to that. {xref="PMID:15329382"} synonym: "microarray RNA expression level evidence" EXACT [] synonym: "REM evidence" EXACT [] synonym: "RNA expression microarray evidence" EXACT [] synonym: "transcript levels (e.g. microarray data)" RELATED [] is_a: ECO:0000058 ! expression microarray evidence [Term] id: ECO:0000105 name: Nimblegen array evidence def: "A type of cDNA to DNA expression microarray evidence resulting from the use of a proprietary NimbleGen array to measure expression levels." [PMID:15607417, url:https\://roche-biochem.jp/pdf/products/microarray/user_guide/SeqCap/SeqCap_UserGuide_Delivery_ver3.0.pdf] is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence [Term] id: ECO:0000106 name: northern blot evidence def: "A type of transcript expression evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples." [ECO:SW, TAIR:TED] synonym: "northern assay evidence" EXACT [] synonym: "RNA blot evidence" EXACT [] synonym: "transcript levels (e.g. Northerns)" RELATED [] is_a: ECO:0000009 ! transcript expression evidence property_value: IAO:0000112 "Northern blot analysis indicated that there was one major band of 1.5-kb (which was used for the stability determination) and two other minor bands of approximately 3.0- and 6.0-kb, which constituted less than 5% of the total signal (Fig. 1C), suggesting that other genes may be cotranscribed along with SMU.1882." xsd:string {xref="PMID:21124877"} property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000109 name: reverse transcription polymerase chain reaction evidence alt_id: ECO:0000108 def: "A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression." [ECO:SW, PMID:11013345, PMID:12901609] comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate. synonym: "reverse transcription polymerase chain reaction transcription evidence" BROAD [] synonym: "RT-PCR" EXACT [] synonym: "transcript levels (e.g. RT-PCR)" RELATED [] is_a: ECO:0005518 ! RNA detection assay evidence property_value: IAO:0000112 "Taken together, the results of the RT-PCR analyses and the reporter fusion assays indicate that expression of SMU.1882 is up regulated in the presence of CovR." xsd:string {xref="PMID:21124877"} property_value: IAO:0000112 "The RT-PCR assay indicated that the sycO, ypkA and yopJ genes (designated as pCD12, pCD13 and pCD14 in Y. pestis 91001 [19], respectively) were transcribed as a single primary RNA (Fig. 1), and thereby these three genes constituted a single operon in Y. pestis Microtus strain 201." xsd:string {xref="PMID:19703315"} property_value: IAO:0000112 "Using reverse transcription-PCR, we found that primers designed to span the intergenic regions between zur and the znuC homolog, as well as znuC and znuB homolog each generated a PCR product (Figure 2A). This indicated that these genes were transcribed on the same mRNA." xsd:string {xref="PMID:24086521"} property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000110 name: RNA protection assay evidence def: "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." [ECO:SW, PMID:23457339, TAIR:TED] synonym: "ribonuclease protection assay" EXACT [] synonym: "RNA protection assay" RELATED [] synonym: "RNAse protection assay" RELATED [] synonym: "RPA" NARROW [] is_a: ECO:0001106 ! nuclease protection assay evidence property_value: IAO:0000112 "fur RPAs were performed on RNA isolated from each strain, and an increase in fur expression was seen for G27, 26695, and the Fur swap strain under iron-depletion shock conditions while little to no increase was seen under iron-limited growth conditions (Fig. 4D and data not shown). This data shows that Fur autoregulation is consistent in each strain and further supports the notion that the AA difference in Fur is not responsible for the difference in sodB regulation between G27 and 26695." xsd:string {xref="PMID:19399190"} property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000112 name: qualitative western immunoblotting evidence alt_id: ECO:0005602 def: "A type of protein expression evidence where proteins are separated using gel electrophoresis, blotted onto a membrane, and probed with an antibody that can be detected via light or radioactive emission." [ECO:SW, PMID:23050259, TAIR:TED] comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies. synonym: "protein expression level evidence based on western blot" EXACT [] synonym: "protein immunoblot" RELATED [] synonym: "protein levels (e.g. Western blots)" RELATED [] synonym: "Western blot expression analysis" RELATED [] is_a: ECO:0000010 ! protein expression evidence is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence is_a: ECO:0005593 ! immunodetection assay evidence property_value: IAO:0000112 "When we determined the proteolysis role of Lons in VapBC10 proteins by Western blot analysis using the strains BL21(DE3)(pJS882) and BL21(DE3)(pJS427), the levels of VapB10 and VapC10 remained stable over the course of translation inhibition (Figure 7D and E). Thus, Lon could not degrade VapBC10 proteins, consistent with our drop growth evidence (Figure 6B)." xsd:string {xref="PMID:24260461"} property_value: IAO:0000234 "CollecTF" xsd:string [Term] id: ECO:0000114 name: expression library screen evidence def: "A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." [ECO:MCC, TAIR:TED] synonym: "expression library screening" RELATED [] is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000116 name: differential hybridization evidence def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue is compared to another cell type or tissue when mRNA from each sample is reverse transcribed, labeled, and hybridized to a cDNA library to compare hybridization patterns." [PMID:14668817] comment: Differential hybridization identifies differentially expressed cloned genes. Two different mRNA-derived probes are used to demonstrate RNA expression under a specific condition. synonym: "differential hybridization" RELATED [] is_a: ECO:0000009 ! transcript expression evidence [Term] id: ECO:0000118 name: subtractive hybridization evidence def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187] comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries. synonym: "subtractive hybridization" RELATED [] is_a: ECO:0000009 ! transcript expression evidence [Term] id: ECO:0000120 name: over expression analysis evidence def: "A type of experimental phenotypic evidence in which a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0005027 ! genetic transformation evidence property_value: IAO:0000112 "In contrast, when RpoQ was overexpressed in either the wild type or the DeltalitR mutant, both were essentially nonmotile (Fig. 7), suggesting that quorum signaling regulates motility, at least in part, through an RpoQ mechanism downstream of LitR." xsd:string {xref="PMID:22233679"} [Term] id: ECO:0000122 name: protein localization evidence def: "A type of localization evidence where sub-cellular localization of a protein is determined." [GO:0008104, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019937] is_a: ECO:0001533 ! localization evidence [Term] id: ECO:0000124 name: fusion protein localization evidence def: "A type of protein localization evidence resulting from the fusion of a protein of interest to a labeling protein which has enzymatic activity or fluorescence properties." [MI:0240] is_a: ECO:0000122 ! protein localization evidence [Term] id: ECO:0000126 name: green fluorescent protein fusion protein localization evidence def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with green fluorescent protein (GFP) from the jellyfish Aequorea victoria." [PMID:9759496] synonym: "GFP fusion protein localization evidence" EXACT [] synonym: "localization of GFP/YFP fusion protein" RELATED [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000128 name: yellow fluorescent protein fusion protein localization evidence def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with yellow fluorescent protein (YFP), which was created by mutating green fluorescent protein (GFP) of the Aequorea victoria." [MI:0368, url:http\://www.jbc.org/content/276/31/29188.long] synonym: "localization of GFP/YFP fusion protein" RELATED [] synonym: "YFP fusion protein localization evidence" EXACT [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000130 name: beta-glucuronidase fusion protein localization evidence def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-glucuronidase (GUS) coding gene as a reporter gene for the localization of a particular gene product." [ECO:RCT] synonym: "GUS fusion protein localization evidence" EXACT [] synonym: "GUS staining evidence" NARROW [] synonym: "localization of GUS fusion protein" RELATED [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000132 name: beta-galactosidase fusion protein localization evidence def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-galactosidase coding gene (lacZ) into a specific gene to create a beta-galactosidase-tagged fusion protein." [PMID:23681629] synonym: "LacZ fusion protein localization evidence" EXACT [] is_a: ECO:0000124 ! fusion protein localization evidence [Term] id: ECO:0000134 name: transport assay evidence def: "A type of direct assay evidence resulting from the activity assessment of transport proteins." [PMID:18401524] synonym: "transport assay" RELATED [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000136 name: nucleic acid binding evidence def: "A type of affinity evidence resulting from the binding of a molecule to a nucleic acid." [GO:0003676] is_a: ECO:0000023 ! affinity evidence [Term] id: ECO:0000138 name: ribohomopolymer binding assay evidence def: "A type of nucleic acid binding evidence resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:PMC102612] synonym: "ribohomopolymer binding assay" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000140 name: thin layer chromatography evidence def: "A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate." [ECO:MCC] synonym: "thin layer chromatography" RELATED [] synonym: "TLC evidence" EXACT [] is_a: ECO:0000325 ! chromatography evidence [Term] id: ECO:0000142 name: protein:ion binding evidence def: "A type of protein binding evidence resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] is_a: ECO:0000024 ! protein binding evidence [Term] id: ECO:0000144 name: Southwestern blot evidence def: "A type of nucleic acid binding evidence in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] synonym: "Southwestern analysis" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000146 name: Northwestern blot evidence def: "A type of nucleic acid binding evidence in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] synonym: "Northwestern analysis" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence [Term] id: ECO:0000148 name: in vitro binding evidence is_obsolete: true [Term] id: ECO:0000150 name: in vitro transcription reconstitution assay evidence def: "A type of reconstitution assay evidence resulting from the use of reconstituted polymerase transcription systems to investigate RNA synthesis." [PMID:9237163] synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] is_a: ECO:0000003 ! reconstitution assay evidence property_value: IAO:0000112 "As shown in Fig. 5, addition of reconstituted RNA polymerase holoenzyme, but not the core enzyme, generated expected size bands of 193-nt and 158-nt when P1882 and Pami, respectively, were used as template (lanes 2 and 7). However, the addition of CovR did not lead to any observable increase in transcription from P1882." xsd:string {xref="PMID:21124877"} [Term] id: ECO:0000152 name: in vitro recombinant protein transcription reconstitution assay evidence def: "A type of in vitro transcription reconstitution assay evidence resulting from the use of recombinant proteins in preparation of a fully-defined transcription system." [PMID:9237165] synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence [Term] id: ECO:0000154 name: heterologous protein expression evidence def: "A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed." [ECO:KAV] synonym: "protein expression in heterologous system" RELATED [] is_a: ECO:0000010 ! protein expression evidence [Term] id: ECO:0000156 name: protein separation evidence def: "A type of fractionation evidence resulting from the isolation of a single type of protein from a complex mixture." [ERO:0000526] is_a: ECO:0000100 ! fractionation evidence [Term] id: ECO:0000158 name: protein separation followed by direct sequencing evidence def: "A type of protein separation evidence that is followed by direct protein sequencing whereby a protein's amino acid sequence is determined experimentally by Edman degradation or by mass spectrometry." [] synonym: "protein separation and direct sequencing" RELATED [] is_a: ECO:0000156 ! protein separation evidence [Term] id: ECO:0000160 name: protein separation followed by fragment identification evidence def: "A type of protein separation evidence which then utilizes the screening and identification of small-molecules that bind to proteins." [https://pubs.acs.org/doi/10.1021/bi3005126, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632530/] synonym: "protein separation and fragment identification" RELATED [] is_a: ECO:0000156 ! protein separation evidence [Term] id: ECO:0000162 name: heterologous system uptake evidence def: "A type of transport assay evidence in which the uptake mechanism of a transporter is investigated in a cell that does not normally express that protein." [doi:10.1146/annurev.pp.46.060195.002223, ECO:RCT] comment: The transporter is a recombinant protein. synonym: "uptake assay in heterologous system" RELATED [] is_a: ECO:0000134 ! transport assay evidence [Term] id: ECO:0000164 name: electrophysiology assay evidence def: "A type of direct assay evidence where electrical properties of cells or tissues are studied." [ECO:KAV] comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart. is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000166 name: two-electrode voltage clamp recording evidence def: "A type of voltage clamp recording evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current." [ECO:SN, PMID:23529422] synonym: "two-electrode voltage clamp technique" RELATED [] is_a: ECO:0005576 ! voltage clamp recording evidence [Term] id: ECO:0000168 name: transcription assay evidence def: "A type of direct assay evidence resulting from the detection and quantification of transcribed RNA." [MeSH:D014158, url:https\://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-15-7] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000170 name: transcriptional activation assay evidence def: "A type of transcription assay evidence in which sequence-specific binding proteins that increase transcription of a specific target gene or genes are identified, quantified, and/or analyzed." [url:http\://smcg.ccg.unam.mx/enp-unam/05-TranscripYRegulacion/transcription.pdf] synonym: "transcriptional activation assay" RELATED [] is_a: ECO:0000168 ! transcription assay evidence [Term] id: ECO:0000172 name: biochemical trait analysis evidence def: "A type of experimental phenotypic evidence in which the biochemical aspect of a mutation is analyzed." [ECO:RCT] comment: For example, the accumulation of a biosynthetic intermediate. synonym: "analysis of biochemical trait" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence [Term] id: ECO:0000174 name: mutant physiological response evidence def: "A type of mutant phenotype evidence in which the physiological response of a mutant when presented with an external stimulus is determined." [ECO:RCT] comment: Examples include: abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions. synonym: "analysis of physiological response" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000176 name: mutant visible phenotype evidence def: "A type of mutant phenotype evidence in which traits arising from a mutation are visually examined." [ECO:RCT] synonym: "analysis of visible trait" RELATED [] is_a: ECO:0000015 ! mutant phenotype evidence [Term] id: ECO:0000177 name: genomic context evidence def: "A type of similarity evidence in which the location of the gene within the genome provides insight into the gene's function or evolutionary insight." [PMID:10958625, PMID:21609955] comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis. is_a: ECO:0000041 ! similarity evidence [Term] id: ECO:0000178 name: in vivo assay evidence def: "A type of direct assay evidence resulting from a sample of microorganisms or living tissue grown in a medium." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=46352] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000179 name: animal model system study evidence def: "A type of experimental evidence arising from the investigation of an animal model system." [ECO:MCC] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000180 name: clinical study evidence def: "A type of experimental evidence arising from a controlled investigation that uses human subjects." [ECO:MCC] is_a: ECO:0000006 ! experimental evidence [Term] id: ECO:0000181 name: in vitro assay evidence alt_id: ECO:0005013 def: "A type of direct assay evidence resulting from a process performed outside the living organism, where the components of an organism have been isolated from their usual biological context, to permit a more detailed or more convenient analysis in an artificial setting." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=45733] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000182 name: in vitro culture assay evidence comment: Use ECO:0001563 (cell growth assay evidence) in place of this term. is_obsolete: true [Term] id: ECO:0000183 name: cell-free assay evidence def: "A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc." [PMID:18453125] synonym: "in vitro assay" BROAD [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0000184 name: enzyme inhibition evidence def: "A type of protein inhibition evidence and enzymatic acitivty evidence where the protein under study is an enzyme." [ECO:MCC] is_a: ECO:0000005 ! enzymatic activity assay evidence is_a: ECO:0000020 ! protein inhibition evidence [Term] id: ECO:0000200 name: sequence alignment evidence alt_id: ECO:00000057 def: "A type of sequence similarity evidence in which an annotation is made based on a manually-reviewed or published sequence alignment" [url:http\://www.geneontology.org/GO.evidence.shtml] comment: Such alignments may be pairwise alignments or multiple alignments. is_a: ECO:0000044 ! sequence similarity evidence created_by: mchibucos creation_date: 2010-03-18T12:21:31Z [Term] id: ECO:0000201 name: sequence orthology evidence alt_id: ECO:00000060 def: "A type of sequence similarity evidence in which orthology is established by multiple criteria, including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." [url:http\://www.geneontology.org/GO.evidence.shtml] comment: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. synonym: "ortholog evidence" RELATED [] is_a: ECO:0000044 ! sequence similarity evidence created_by: mchibucos creation_date: 2010-03-18T12:30:06Z [Term] id: ECO:0000202 name: match to sequence model evidence alt_id: ECO:00000063 def: "A type of sequence similarity evidence in which the function of a gene product is predicted based on a sequence-based statistical model." [ECO:MCC] {comment="url:http://www.geneontology.org/GO.evidence.shtml"} comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN. is_a: ECO:0000044 ! sequence similarity evidence property_value: IAO:0000112 "A putative rho-independent terminator sequence (GCCTGACTACATAGATGTCAGGC) was identified at position 402-bp downstream of SMU.1882 by TransTerm (transterm.dev.java.net) program." xsd:string {xref="PMID:21124877"} created_by: mchibucos creation_date: 2010-03-18T12:32:30Z [Term] id: ECO:0000203 name: automatic assertion alt_id: ECO:00000067 def: "An assertion method that does not involve human review." [ECO:MCC] comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically. is_a: ECO:0000217 ! assertion method disjoint_from: ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-03-18T12:36:04Z [Term] id: ECO:0000204 name: author statement alt_id: ECO:0007010 def: "A type of documented statement evidence that is based on an assertion by the author of a paper, which is read by a curator." [ECO:MCC] is_a: ECO:0006151 ! documented statement evidence union_of: ECO:0000033 ! author statement supported by traceable reference union_of: ECO:0000034 ! author statement without traceable support created_by: mchibucos creation_date: 2010-06-21T11:17:21Z [Term] id: ECO:0000205 name: curator inference def: "A type of inferential evidence that is based on a conclusion drawn by a curator." [ECO:MCC] is_a: ECO:0000361 ! inferential evidence created_by: mchibucos creation_date: 2010-08-18T05:55:12Z [Term] id: ECO:0000206 name: BLAST evidence def: "A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST)." [ECO:MCC] is_a: ECO:0005554 ! pairwise sequence alignment evidence created_by: mchibucos creation_date: 2010-08-06T04:48:24Z [Term] id: ECO:0000207 name: nucleotide BLAST evidence def: "A type of BLAST evidence in which nucleotide sequences are aligned." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence created_by: mchibucos creation_date: 2010-08-06T04:49:58Z [Term] id: ECO:0000208 name: protein BLAST evidence def: "A type of BLAST evidence in which amino acid or translated nucleotide sequences are aligned." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence property_value: IAO:0000112 "Blast search of S. meliloti genome for homologues of X. campestris Ohr protein revealed two paralogues, SMa2389 and SMc00040, showing 52 and 57% identity respectively with Ohr of X. campestris." xsd:string {xref="PMID:21569462"} created_by: mchibucos creation_date: 2010-08-06T04:50:45Z [Term] id: ECO:0000209 name: BLAST evidence used in automatic assertion def: "A type of BLAST evidence that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000206 ! BLAST evidence is_a: ECO:0005561 {is_inferred="true"} ! pairwise sequence alignment evidence used in automatic assertion intersection_of: ECO:0000206 ! BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-08-06T04:55:21Z [Term] id: ECO:0000210 name: nucleotide BLAST evidence used in automatic assertion def: "A type of nucleotide BLAST evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000207 ! nucleotide BLAST evidence is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion intersection_of: ECO:0000207 ! nucleotide BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-08-06T05:09:51Z [Term] id: ECO:0000211 name: protein BLAST evidence used in automatic assertion def: "A type of protein BLAST evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000208 ! protein BLAST evidence is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion intersection_of: ECO:0000208 ! protein BLAST evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-08-06T05:11:04Z [Term] id: ECO:0000212 name: combinatorial evidence alt_id: ECO:0000036 alt_id: ECO:0000043 def: "A type of evidence in which at least two distinct types of evidence have been integrated." [ECO:MCC, ECO:RCT] comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence. synonym: "inferred from in-silico analysis" RELATED [] is_a: ECO:0000000 ! evidence intersection_of: ECO:0000000 ! evidence intersection_of: BFO:0000051 ECO:0000000 ! evidence created_by: mchibucos creation_date: 2010-08-06T05:24:57Z [Term] id: ECO:0000213 name: combinatorial evidence used in automatic assertion def: "A type of combinatorial analysis that is used in an automatic assertion." [ECO:MCC] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. synonym: "inferred from in-silico analysis" RELATED [] is_a: ECO:0000212 ! combinatorial evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-08-06T05:28:33Z [Term] id: ECO:0000214 name: biological aspect of descendant evidence def: "A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene." [ECO:MCC] comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T11:16:50Z [Term] id: ECO:0000215 name: rapid divergence from ancestral sequence evidence def: "A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event." [ECO:MCC] comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T03:41:34Z [Term] id: ECO:0000216 name: phylogenetic determination of loss of key residues evidence def: "A type of phylogenetic evidence characterized by the absence of key sequence residues." [ECO:MCC] comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2010-10-05T03:43:17Z [Term] id: ECO:0000217 name: assertion method def: "A means by which a statement is made about an entity." [ECO:MCC] union_of: ECO:0000203 ! automatic assertion union_of: ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-11-12T04:17:37Z [Term] id: ECO:0000218 name: manual assertion def: "An assertion method that involves human review." [ECO:MCC] comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information. is_a: ECO:0000217 ! assertion method created_by: mchibucos creation_date: 2010-11-12T04:20:02Z [Term] id: ECO:0000219 name: nucleotide sequencing assay evidence def: "A type of sequencing assay that determines the sequence of a biopolymer comprised of nucleotides." [ECO:MCC] synonym: "DNA sequencing" RELATED [OBI:0000626] is_a: ECO:0000220 ! sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T12:22:40Z [Term] id: ECO:0000220 name: sequencing assay evidence def: "A type of experimental evidence where the order of molecules that constitute a biopolymer is determined." [ECO:MCC, OBI:0600047] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2010-11-15T01:44:45Z [Term] id: ECO:0000221 name: high throughput nucleotide sequencing assay evidence def: "A type of nucleotide sequencing assay evidence in which a sequence is derived from a parallelized sequencing technique." [ECO:MCC] comment: Typically thousands to millions of reads are produced in parallel. synonym: "next generation sequencing evidence" RELATED [] is_a: ECO:0000219 ! nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T02:19:34Z [Term] id: ECO:0000222 name: Illumina sequencing evidence def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed by progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage." [OBI:0000724, PMID:26000844] comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging. synonym: "Solexa sequencing result" RELATED [OBI:0000724] is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T02:38:03Z [Term] id: ECO:0000223 name: 454 pyrosequencing evidence def: "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." [EFO:0005016] comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR. synonym: "pyrosequencing" BROAD [OBI:0000730] is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T03:50:14Z [Term] id: ECO:0000224 name: SOLiD sequencing evidence def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through rounds of ligation-based sequencing with fluorescently-labeled Di-base probes and cleavage, after which the template is reset, with a primer offset by one base, for subsequent rounds of ligation." [OBI:0000706] comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag.\n\nSamples are prepared by fragmentation of a sample library, adaptor ligation, emulsion PCR, and attachment of resultant beads to glass slides. is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T04:17:41Z [Term] id: ECO:0000225 name: chain termination sequencing evidence def: "A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged." [OBI:0000695] comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.\n\nThe ddNTPs lack an OH group so the next dNTP cannot be attached, causing chain termination. synonym: "dye terminator sequencing" EXACT [] synonym: "Sanger sequencing" EXACT [] is_a: ECO:0000219 ! nucleotide sequencing assay evidence created_by: mchibucos creation_date: 2010-11-15T04:46:31Z [Term] id: ECO:0000226 name: chromatin immunoprecipitation evidence def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC] comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). synonym: "ChIP evidence" EXACT [] xref: MI:0402 "chromatin immunoprecipitation assay" is_a: ECO:0000085 ! immunoprecipitation evidence created_by: mchibucos creation_date: 2010-11-16T01:29:08Z [Term] id: ECO:0000227 name: chromatin immunoprecipitation-PCR evidence def: "A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection." [ECO:MCC, ECO:SW, PMID:18388953] comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results. synonym: "ChIP-PCR evidence" EXACT [] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: mchibucos creation_date: 2010-11-16T05:02:00Z [Term] id: ECO:0000228 name: chromatin immunoprecipitation-qPCR evidence def: "A type of chromatin immunoprecipitation evidence in which protein-DNA interactions are investigated at known genomic binding sites through a process of ChIP, DNA purification, and finally quantitative polymerase chain reaction (qPCR) for DNA detection." [url:https\://www.diagenode.com/applications/chip-qpcr] synonym: "ChIP-qPCR evidence" EXACT [] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence property_value: IAO:0000112 "To confirm the in vivo binding of FimR to pfim, and to determine the impact of Mn2+ and Fe2+ on the binding activity of FimR, chromatin immunoprecipitation (ChIP) assay-quantitative real time PCR (qPCR) with anti-FimR antibody was employed as detailed in the materials and methods. The strongest binding of FimR to pfim was detected in cells grown in the presence of 50 microM MnCl2 and 50 microM FeSO4 (Figure 5, lane IV), whereas minimal amounts of MnCl2 (0.01 microM) and FeSO4 (0.1 microM) led to the weakest binding (Figure 5, lane I)." xsd:string {xref="PMID:23823757"} created_by: mchibucos creation_date: 2010-11-16T05:26:47Z [Term] id: ECO:0000229 name: chromatin immunoprecipitation-seq evidence def: "A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins." [ECO:MCC, ECO:SW, OBI:0000716, PMID:22955991] comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used. synonym: "ChIP-SEQ evidence" EXACT [OBI:0000716] synonym: "ChIP-seq evidence" EXACT [PMID:22955991] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: mchibucos creation_date: 2010-11-16T05:30:41Z [Term] id: ECO:0000230 name: chromatin immunoprecipitation-chip evidence def: "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927] comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp. synonym: "ChIP-chip evidence" EXACT [] synonym: "ChIP-on-chip evidence" EXACT [] xref: MI:0225 "chromatin immunoprecipitation array" is_a: ECO:0000226 ! chromatin immunoprecipitation evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: mchibucos creation_date: 2010-11-16T05:43:55Z [Term] id: ECO:0000231 name: quantitative polymerase chain reaction evidence def: "A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW] comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\n\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).\n\nqPCR can be used to quantitatively determine levels of gene expression. synonym: "Q-PCR evidence" EXACT [] synonym: "qPCR evidence" EXACT [] synonym: "qRT-PCR evidence" RELATED [] synonym: "quantitative PCR evidence" EXACT [] synonym: "quantitative real-time PCR evidence" EXACT [] synonym: "real time polymerase chain reaction evidence" EXACT [] synonym: "real-time PCR evidence" EXACT [] synonym: "real-time quantitative PCR" EXACT [] is_a: ECO:0005519 ! DNA detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: mchibucos creation_date: 2010-11-16T05:57:20Z [Term] id: ECO:0000232 name: chromosome conformation-based evidence def: "A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, DNA processing, and quantitation that serves to analyze the spatial organization of chromatin in a cell and quantify the number of interactions between genomic loci that are nearby in 3-D space." [ECO:MCC] comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C. synonym: "3C" NARROW [] synonym: "chromosome conformation capture" NARROW [] synonym: "chromosome conformation capture-based evidence" EXACT [] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2010-11-17T12:27:14Z [Term] id: ECO:0000233 name: 3C evidence def: "A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step." [ECO:MCC] comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation. synonym: "3C" EXACT [] synonym: "chromosome conformation capture evidence" EXACT [] synonym: "chromosome conformation capture-PCR evidence" NARROW [] is_a: ECO:0000232 ! chromosome conformation-based evidence created_by: mchibucos creation_date: 2010-11-17T12:46:15Z [Term] id: ECO:0000234 name: 4C evidence def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." [ECO:MCC, PMID:17033624] comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation. synonym: "chromosome conformation capture on chip" EXACT [] synonym: "circularized 3C" EXACT [] synonym: "circularized chromosome conformation capture evidence" EXACT [] is_a: ECO:0000232 ! chromosome conformation-based evidence created_by: mchibucos creation_date: 2010-11-19T01:20:28Z [Term] id: ECO:0000235 name: 5C evidence def: "A type of chromosome conformation-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation." [ECO:MCC] comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation. synonym: "5C" EXACT [] synonym: "carbon-copy 3C" EXACT [] synonym: "carbon-copy chromosome conformation capture evidence" EXACT [] is_a: ECO:0000232 ! chromosome conformation-based evidence created_by: mchibucos creation_date: 2010-11-19T01:22:20Z [Term] id: ECO:0000236 name: chromosome conformation capture-PCR evidence def: "A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step." [ECO:MCC] comment: See 3C evidence. 3C evidence was initially developed using PCR. synonym: "3C" BROAD [] synonym: "3C-PCR" EXACT [] is_obsolete: true created_by: mchibucos creation_date: 2010-11-19T03:00:47Z [Term] id: ECO:0000237 name: 3C-qPCR evidence def: "A type of 3C evidence evidence that 1) quantifies interactions between a single pair of genomic loci (not multiple interactions) and 2) performs quantitative polymerase chain reaction (qPCR) in the quantitation step." [ECO:MCC, PMID:17641637, PMID:26025624] synonym: "3C-quantitative PCR" EXACT [] synonym: "3C-real-time PCR" EXACT [] synonym: "chromosome conformation capture-qPCR evidence" EXACT [] is_a: ECO:0000233 ! 3C evidence created_by: mchibucos creation_date: 2010-11-19T03:06:47Z [Term] id: ECO:0000238 name: Hi-C evidence def: "A type of chromosome conformation-based evidence that is derived by performing immunoprecipitation enrichment of the biotin labelled ligated fragments events prior to sequencing of the library and mapping it to the genome." [ECO:MCC] comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step. is_a: ECO:0000232 ! chromosome conformation-based evidence created_by: mchibucos creation_date: 2010-11-19T04:24:03Z [Term] id: ECO:0000239 name: 3C-seq evidence def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing." [ECO:MCC, PMID:23411633] comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation. synonym: "3C sequencing" EXACT [] synonym: "chromosome conformation capture sequencing evidence" RELATED [] synonym: "circularized 3C sequencing" EXACT [] synonym: "circularized 3C-seq" EXACT [] is_a: ECO:0000232 ! chromosome conformation-based evidence created_by: mchibucos creation_date: 2010-11-30T03:22:25Z [Term] id: ECO:0000240 name: anatomical perturbation phenotypic evidence def: "A type of experimental phenotypic evidence resulting from the disruption of a structural feature." [ECO:MCC] synonym: "anatomical perturbation evidence" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:24:16Z [Term] id: ECO:0000241 name: environmental perturbation phenotypic evidence def: "A type of experimental phenotypic evidence resulting from modification to the surroundings or conditions in which an organism lives or operates." [ECO:MCC] comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth. synonym: "environmental perturbation evidence" EXACT [] synonym: "mechanical constraint evidence" RELATED [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000242 name: tissue ablation phenotypic evidence def: "A type of anatomical perturbation phenotypic evidence resulting from the surgical removal of tissue or subcellular components." [ECO:MCC] synonym: "ablated tissue evidence" EXACT [] synonym: "tissue ablation evidence" EXACT [] is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000243 name: tissue grafting phenotypic evidence def: "A type of anatomical perturbation phenotypic evidence resulting from the addition of tissue to an organism through a graft procedure." [ECO:MCC] synonym: "tissue grafting evidence" EXACT [] is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence created_by: mchibucos creation_date: 2010-12-06T12:33:58Z [Term] id: ECO:0000244 name: combinatorial evidence used in manual assertion def: "A type of combinatorial analysis that is used in a manual assertion." [ECO:MCC] comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. synonym: "inferred from in-silico analysis" RELATED [] is_a: ECO:0000212 ! combinatorial evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-12-09T02:02:12Z [Term] id: ECO:0000245 name: automatically integrated combinatorial evidence used in manual assertion def: "A type of automatically integrated combinatorial evidence that is used in a manual assertion." [ECO:RCT] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "computational combinatorial evidence used in manual assertion" RELATED [] synonym: "inferred from reviewed computational analysis" EXACT [GOECO:RCA] synonym: "RCA" EXACT [GOECO:RCA] xref: GOECO:RCA "inferred from reviewed computational analysis" is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion is_a: ECO:0007673 {is_inferred="true"} ! automatically integrated combinatorial evidence intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "RCA" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/rca-inferred-reviewed-computational-analysis xsd:string created_by: mchibucos creation_date: 2010-12-09T02:37:54Z [Term] id: ECO:0000246 name: computational combinatorial evidence used in automatic assertion def: "A type of computational combinatorial analysis that is used in an automatic assertion." [ECO:MCC] comment: Merged with ECO:0000053. is_obsolete: true created_by: mchibucos creation_date: 2010-12-09T02:37:54Z [Term] id: ECO:0000247 name: sequence alignment evidence used in manual assertion def: "A type of sequence alignment evidence that is used in a manual assertion." [ECO:RCT] subset: valid_with_gene subset: valid_with_protein synonym: "inferred from sequence alignment" EXACT [GOECO:ISA] synonym: "ISA" EXACT [GOECO:ISA] xref: GOECO:ISA "inferred from sequence alignment" is_a: ECO:0000200 ! sequence alignment evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000200 ! sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/isa-inferred-sequence-alignment xsd:string created_by: mchibucos creation_date: 2010-12-09T05:12:27Z [Term] id: ECO:0000248 name: sequence alignment evidence used in automatic assertion def: "A type of sequence alignment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000200 ! sequence alignment evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000200 ! sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-09T05:12:27Z [Term] id: ECO:0000249 name: sequence similarity evidence used in automatic assertion def: "A type of sequence similarity evidence that is used in an automatic assertion." [ECO:RCT] xref: GO_REF:0000107 "Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl" is_a: ECO:0000044 ! sequence similarity evidence is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion intersection_of: ECO:0000044 ! sequence similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-09T05:22:30Z [Term] id: ECO:0000250 name: sequence similarity evidence used in manual assertion def: "A type of sequence similarity evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from sequence or structural similarity" EXACT [GOECO:ISS] synonym: "ISS" EXACT [GOECO:ISS] xref: GOECO:ISS "inferred from sequence or structural similarity" is_a: ECO:0000044 ! sequence similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion is_a: ECO:0007668 {is_inferred="true"} ! computational evidence used in manual assertion intersection_of: ECO:0000044 ! sequence similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISS" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/iss-inferred-sequence-or-structural-similarity xsd:string created_by: mchibucos creation_date: 2010-12-09T05:22:30Z [Term] id: ECO:0000251 name: similarity evidence used in automatic assertion def: "A type of similarity evidence that is used in an automatic assertion." [ECO:RCT] synonym: "inferred from similarity" RELATED [] synonym: "IS" BROAD [] is_a: ECO:0000041 ! similarity evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000041 ! similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-09T05:28:12Z [Term] id: ECO:0000252 name: similarity evidence used in manual assertion def: "A type of similarity evidence that is used in a manual assertion." [ECO:RCT] subset: go_groupings synonym: "inferred from similarity" RELATED [] synonym: "IS" BROAD [] is_a: ECO:0000041 ! similarity evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000041 ! similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-12-09T05:28:12Z [Term] id: ECO:0000253 name: genetic similarity evidence used in manual assertion def: "A type of genetic similarity evidence that is used in a manual assertion." [ECO:RCT] synonym: "IGTS" BROAD [] synonym: "inferred from genetic similarity" RELATED [] is_a: ECO:0000051 ! genetic similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000051 ! genetic similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2010-12-10T01:42:44Z [Term] id: ECO:0000254 name: genetic similarity evidence used in automatic assertion def: "A type of genetic similarity evidence that is used in an automatic assertion." [ECO:RCT] synonym: "IGTS" BROAD [] synonym: "inferred from genetic similarity" RELATED [] is_a: ECO:0000051 ! genetic similarity evidence is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion intersection_of: ECO:0000051 ! genetic similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T01:42:44Z [Term] id: ECO:0000255 name: match to sequence model evidence used in manual assertion def: "A type of match to sequence model evidence that is used in a manual assertion." [ECO:RCT] synonym: "inferred from sequence model" EXACT [GOECO:ISM] synonym: "ISM" EXACT [GOECO:ISM] xref: GO_REF:0000011 "Hidden Markov Models (TIGR)" xref: GOECO:ISM "inferred from sequence model" is_a: ECO:0000202 ! match to sequence model evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000202 ! match to sequence model evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ism-inferred-sequence-model xsd:string created_by: mchibucos creation_date: 2010-12-10T02:26:35Z [Term] id: ECO:0000256 name: match to sequence model evidence used in automatic assertion def: "A type of match to sequence model evidence that is used in an automatic assertion." [ECO:RCT] xref: GO_REF:0000002 "Gene Ontology annotation through association of InterPro records with GO terms." is_a: ECO:0000202 ! match to sequence model evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000202 ! match to sequence model evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T02:26:35Z [Term] id: ECO:0000257 name: motif similarity evidence used in manual assertion def: "A type of motif similarity evidence that is used in a manual assertion." [ECO:RCT] synonym: "recognized domains" RELATED [] is_a: ECO:0000028 ! motif similarity evidence is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion intersection_of: ECO:0000028 ! motif similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: mchibucos creation_date: 2010-12-10T02:44:34Z [Term] id: ECO:0000258 name: motif similarity evidence used in automatic assertion def: "A type of motif similarity evidence that is used in an automatic assertion." [ECO:RCT] synonym: "recognized domains" RELATED [] is_a: ECO:0000028 ! motif similarity evidence is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion intersection_of: ECO:0000028 ! motif similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T02:44:34Z [Term] id: ECO:0000259 name: match to InterPro member signature evidence used in automatic assertion def: "A type of match to InterPro member signature evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000029 ! match to InterPro member signature evidence is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion intersection_of: ECO:0000029 ! match to InterPro member signature evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T02:54:51Z [Term] id: ECO:0000260 name: match to InterPro member signature evidence used in manual assertion def: "A type of match to InterPro member signature evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000029 ! match to InterPro member signature evidence is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion intersection_of: ECO:0000029 ! match to InterPro member signature evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: mchibucos creation_date: 2010-12-10T02:54:51Z [Term] id: ECO:0000261 name: targeting sequence prediction evidence used in automatic assertion def: "A type of targeting sequence prediction that is used in an automatic assertion." [ECO:RCT] synonym: "targeting sequence prediction" RELATED [] is_a: ECO:0000081 ! targeting sequence prediction evidence is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion intersection_of: ECO:0000081 ! targeting sequence prediction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T03:02:49Z [Term] id: ECO:0000262 name: targeting sequence prediction evidence used in manual assertion def: "A type of targeting sequence prediction that is used in a manual assertion." [ECO:RCT] synonym: "targeting sequence prediction" RELATED [] is_a: ECO:0000081 ! targeting sequence prediction evidence is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion intersection_of: ECO:0000081 ! targeting sequence prediction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: mchibucos creation_date: 2010-12-10T03:02:49Z [Term] id: ECO:0000263 name: transmembrane domain prediction evidence used in automatic assertion def: "A type of transmembrane domain prediction that is used in an automatic assertion." [ECO:RCT] synonym: "transmembrane domain prediction" RELATED [] is_a: ECO:0000083 ! transmembrane domain prediction evidence is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion intersection_of: ECO:0000083 ! transmembrane domain prediction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T03:28:35Z [Term] id: ECO:0000264 name: transmembrane domain prediction evidence used in manual assertion def: "A type of transmembrane domain prediction that is used in a manual assertion." [ECO:RCT] synonym: "transmembrane domain prediction" RELATED [] is_a: ECO:0000083 ! transmembrane domain prediction evidence is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion intersection_of: ECO:0000083 ! transmembrane domain prediction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: mchibucos creation_date: 2010-12-10T03:28:35Z [Term] id: ECO:0000265 name: sequence orthology evidence used in automatic assertion def: "A type of sequence orthology evidence that is used in an automatic assertion." [ECO:RCT] synonym: "Ortholog evidence" RELATED [] synonym: "ortholog evidence" RELATED [] xref: GO_REF:0000035 "Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara" is_a: ECO:0000201 ! sequence orthology evidence is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion intersection_of: ECO:0000201 ! sequence orthology evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2010-12-10T03:39:41Z [Term] id: ECO:0000266 name: sequence orthology evidence used in manual assertion def: "A type of sequence orthology evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from sequence orthology" EXACT [] {name="GOECO:ISO"} synonym: "ISO" EXACT [GOECO:ISO] synonym: "Ortholog evidence" RELATED [] synonym: "ortholog evidence" RELATED [] xref: GOECO:ISO "inferred from sequence orthology" is_a: ECO:0000201 ! sequence orthology evidence is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion intersection_of: ECO:0000201 ! sequence orthology evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISO" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/iso-inferred-sequence-orthology xsd:string created_by: mchibucos creation_date: 2010-12-10T03:39:41Z [Term] id: ECO:0000267 name: enzyme-linked immunoabsorbent assay evidence def: "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." [ECO:MCC, ECO:SW, PMID:23949770] comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein. synonym: "ELISA evidence" EXACT [] synonym: "enzyme-linked immunosorbent assay" NARROW [] xref: MI:0411 "enzyme linked immunosorbent assay" is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: mchibucos creation_date: 2010-12-14T01:57:13Z [Term] id: ECO:0000268 name: flow cytometry evidence def: "A type of direct assay evidence in which cells or particles are characterized by passing them through a light source and monitoring the resultant light scatter or fluorochrome excitation." [ECO:MCC] synonym: "FCM" EXACT [] is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2010-12-14T02:07:08Z [Term] id: ECO:0000269 name: experimental evidence used in manual assertion def: "A type of experimental evidence that is used in a manual assertion." [ECO:MCC] synonym: "EXP" EXACT [GOECO:EXP] synonym: "inferred from experiment" EXACT [GOECO:EXP] xref: GOECO:EXP "inferred from experiment" is_a: ECO:0000006 ! experimental evidence is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion intersection_of: ECO:0000006 ! experimental evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/exp-inferred-experiment xsd:string created_by: mchibucos creation_date: 2010-12-20T05:36:49Z [Term] id: ECO:0000270 name: expression pattern evidence used in manual assertion def: "A type of expression pattern evidence that is used in a manual assertion." [ECO:MCC] synonym: "IEP" EXACT [GOECO:IEP] synonym: "inferred from expression pattern" EXACT [GOECO:IEP] xref: GOECO:IEP "inferred from expression pattern" is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000008 ! expression pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/iep-inferred-expression-pattern xsd:string created_by: mchibucos creation_date: 2010-12-20T05:46:39Z [Term] id: ECO:0000271 name: array experiment evidence used in manual assertion comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000273, ECO:0000276, ECO:0000285, etc.) is_obsolete: true created_by: mchibucos creation_date: 2010-12-20T05:51:58Z [Term] id: ECO:0000272 name: Affymetrix GeneChip evidence used in manual assertion def: "A type of Affymetrix GeneChip evidence that is used in a manual assertion." [ECO:MCC] synonym: "Affymetrix array experiment evidence" BROAD [] is_a: ECO:0000101 ! Affymetrix GeneChip evidence is_a: ECO:0000276 {is_inferred="true"} ! cRNA to DNA expression microarray evidence used in manual assertion intersection_of: ECO:0000101 ! Affymetrix GeneChip evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T05:53:36Z [Term] id: ECO:0000273 name: cDNA to DNA expression microarray evidence used in manual assertion alt_id: ECO:0001178 alt_id: ECO:0005525 def: "A type of cDNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] synonym: "DNA microarray|RNA microarray" RELATED [] is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion intersection_of: ECO:0000097 ! cDNA to DNA expression microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T05:55:49Z [Term] id: ECO:0000274 name: differential methylation hybridization evidence used in manual assertion def: "A type of differential methylation hybridization evidence that is used in a manual assertion." [ECO:RCT] synonym: "CpG island microarray evidence" BROAD [] synonym: "CpG island microarray evidence used in manual assertion" EXACT [] is_a: ECO:0000069 ! differential methylation hybridization evidence is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion intersection_of: ECO:0000069 ! differential methylation hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: mchibucos creation_date: 2010-12-20T05:58:44Z [Term] id: ECO:0000275 name: expression microarray evidence used in manual assertion def: "A type of expression microarray evidence that is used in a manual assertion." [ECO:MCC] synonym: "differential gene expression evidence from microarray experiment" RELATED [] is_a: ECO:0000058 ! expression microarray evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000058 ! expression microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:01:26Z [Term] id: ECO:0000276 name: cRNA to DNA expression microarray evidence used in manual assertion def: "A type of cRNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:RCT] synonym: "genomic microarray evidence" BROAD [] synonym: "genomic microarray evidence used in manual assertion" EXACT [] is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion intersection_of: ECO:0000062 ! cRNA to DNA expression microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:03:28Z [Term] id: ECO:0000277 name: Nimblegen array evidence used in manual assertion def: "A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000105 ! Nimblegen array evidence is_a: ECO:0000273 {is_inferred="true"} ! cDNA to DNA expression microarray evidence used in manual assertion intersection_of: ECO:0000105 ! Nimblegen array evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:05:20Z [Term] id: ECO:0000278 name: array-based sequence capture evidence used in manual assertion def: "A type of array-based sequence capture evidence that is used in a manual assertion." [ECO:MCC] synonym: "DNA microarray" BROAD [] synonym: "oligonucleotide microarray evidence" BROAD [] synonym: "oligonucleotide microarray evidence used in manual assertion" BROAD [] synonym: "single nucleotide polymorphism array evidence" RELATED [] synonym: "SNP array evidence" RELATED [] is_a: ECO:0000093 ! array-based sequence capture evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000093 ! array-based sequence capture evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:09:45Z [Term] id: ECO:0000279 name: qualitative western immunoblotting evidence used in manual assertion alt_id: ECO:0007183 def: "A type of qualitative western immunoblotting evidence that is used in a manual assertion." [ECO:MCC] synonym: "protein expression level evidence based on western blot used in manual assertion" EXACT [] synonym: "protein immunoblot" RELATED [] synonym: "protein levels (e.g. Western blots)" RELATED [] synonym: "Western blot expression analysis" RELATED [] is_a: ECO:0000112 ! qualitative western immunoblotting evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000112 ! qualitative western immunoblotting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:20:01Z [Term] id: ECO:0000281 name: expression library screen evidence used in manual assertion def: "A type of expression library screen evidence that is used in a manual assertion." [ECO:MCC] synonym: "expression library screening" RELATED [] is_a: ECO:0000114 ! expression library screen evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000114 ! expression library screen evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:24:05Z [Term] id: ECO:0000282 name: heterologous protein expression evidence used in manual assertion def: "A type of heterologous protein expression analysis that is used in a manual assertion." [ECO:MCC] synonym: "protein expression in heterologous system" RELATED [] is_a: ECO:0000154 ! heterologous protein expression evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000154 ! heterologous protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:27:04Z [Term] id: ECO:0000283 name: spatial pattern of protein expression evidence used in manual assertion def: "A type of protein expression spatial pattern analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000045 ! spatial pattern of protein expression evidence is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion intersection_of: ECO:0000045 ! spatial pattern of protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:31:44Z [Term] id: ECO:0000284 name: protein expression evidence used in manual assertion alt_id: ECO:0000280 def: "A type of protein expression analysis that is used in a manual assertion." [ECO:MCC] synonym: "protein expression level evidence used in manual assertion" EXACT [] is_a: ECO:0000010 ! protein expression evidence is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion intersection_of: ECO:0000010 ! protein expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:33:46Z [Term] id: ECO:0000285 name: DNA to cDNA expression microarray evidence used in manual assertion def: "A type of DNA to cDNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] synonym: "microarray RNA expression level evidence" BROAD [] synonym: "REM evidence" BROAD [] synonym: "RNA expression microarray evidence" BROAD [] synonym: "transcript levels (e.g. microarray data)" RELATED [] is_a: ECO:0000104 ! DNA to cDNA expression microarray evidence is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion intersection_of: ECO:0000104 ! DNA to cDNA expression microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:41:33Z [Term] id: ECO:0000287 name: differential hybridization evidence used in manual assertion def: "A type of differential hybridization experiment that is used in a manual assertion." [ECO:MCC] synonym: "differential hybridization" RELATED [] is_a: ECO:0000116 ! differential hybridization evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000116 ! differential hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:55:14Z [Term] id: ECO:0000288 name: RNA protection assay evidence used in manual assertion def: "A type of RNA protection assay that is used in a manual assertion." [ECO:MCC] synonym: "ribonuclease protection assay" BROAD [] synonym: "RNA protection assay|RPA" RELATED [] synonym: "RNAse protection assay" RELATED [] is_a: ECO:0000110 ! RNA protection assay evidence is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion intersection_of: ECO:0000110 ! RNA protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:57:33Z [Term] id: ECO:0000289 name: spatial pattern of transcript expression evidence used in manual assertion def: "A type of spatial pattern of transcript expression analysis that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000047 ! spatial pattern of transcript expression evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000047 ! spatial pattern of transcript expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T06:59:18Z [Term] id: ECO:0000290 name: subtractive hybridization evidence used in manual assertion def: "A type of subtractive hybridization experiment that is used in a manual assertion." [ECO:MCC] synonym: "subtractive hybridization" RELATED [] is_a: ECO:0000118 ! subtractive hybridization evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000118 ! subtractive hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T07:00:48Z [Term] id: ECO:0000291 name: transcript expression evidence used in manual assertion alt_id: ECO:0000286 def: "A type of transcript expression evidence that is used in a manual assertion." [ECO:MCC] synonym: "transcript expression level evidence" BROAD [] synonym: "transcriptomics evidence used in manual assertion" EXACT [] is_a: ECO:0000009 ! transcript expression evidence is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion intersection_of: ECO:0000009 ! transcript expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2010-12-20T07:02:08Z [Term] id: ECO:0000292 name: morpholino experiment evidence def: "A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell." [ECO:MCC] comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method. synonym: "anti-sense" BROAD [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: mchibucos creation_date: 2011-01-10T03:36:25Z [Term] id: ECO:0000293 name: systematic evolution of ligands by exponential amplification evidence def: "A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC, url:http\://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment] comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein. synonym: "in vitro evolution evidence" RELATED [] synonym: "in vitro selection evidence" EXACT [] synonym: "SELEX evidence" EXACT [] xref: MI:0657 "systematic evolution of ligands by exponential enrichment" is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000112 "A total of 160 SELEX fragments were cloned and sequenced, which were classified into 29 CRP-binding sequences, including 13 previously identified loci and 16 newly identified sites (Table S2). When the CRP-binding sequences are located upstream or near promoters of the flanking genes, these genes are predicted to be under the control of CRP." xsd:string {xref="PMID:21673794"} created_by: mchibucos creation_date: 2011-01-10T04:23:50Z [Term] id: ECO:0000294 name: bacterial one-hybrid evidence def: "A type of bait-prey hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes. synonym: "B1H evidence" EXACT [] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence property_value: IAO:0000112 "Bacterial one-hybrid assays confirmed the interaction between MtrA and the regulatory sequence of the dnaA initiator gene." xsd:string {xref="PMID:20843371"} created_by: mchibucos creation_date: 2011-01-10T06:16:20Z [Term] id: ECO:0000295 name: RNA-sequencing evidence alt_id: ECO:0000357 def: "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. synonym: "differential gene expression evidence from RNA-seq experiment" NARROW [] synonym: "RNA sequencing" RELATED [] synonym: "RNA-seq evidence" EXACT [] synonym: "RNAseq evidence" EXACT [] synonym: "whole transcriptome shotgun sequencing" EXACT [PMID:18611170] synonym: "WTSS" EXACT [] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-01-11T12:54:50Z [Term] id: ECO:0000298 name: cleavage arrested development evidence def: "A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked." [ECO:MCC] comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest. is_a: ECO:0000059 ! experimental phenotypic evidence created_by: mchibucos creation_date: 2011-01-11T03:28:49Z [Term] id: ECO:0000299 name: cytochalasin experiment evidence def: "A type of cleavage arrested development that arises after treatment with cytochalasin." [ECO:MCC] comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments. is_a: ECO:0000298 ! cleavage arrested development evidence created_by: mchibucos creation_date: 2011-01-11T03:30:29Z [Term] id: ECO:0000300 name: green fluorescent protein immunolocalization evidence def: "A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker." [ECO:MCC] comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript". synonym: "GFP immunolocalization evidence" EXACT [] is_a: ECO:0000087 ! immunolocalization evidence created_by: mchibucos creation_date: 2011-01-11T03:48:50Z [Term] id: ECO:0000301 name: beta-galactosidase protein immunolocalization evidence def: "A type of immunolocalization evidence that was generated using LacZ protein as a marker." [ECO:MCC] comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript". synonym: "LacZ protein immunolocalization evidence" EXACT [] is_a: ECO:0000087 ! immunolocalization evidence created_by: mchibucos creation_date: 2011-01-11T04:06:40Z [Term] id: ECO:0000302 name: author statement used in manual assertion alt_id: ECO:0007764 def: "A type of author statement that is used in a manual assertion." [ECO:MCC] synonym: "author inference used in manual assertion" EXACT [] is_a: ECO:0000204 ! author statement is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion intersection_of: ECO:0000204 ! author statement intersection_of: used_in ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2011-01-20T02:26:47Z [Term] id: ECO:0000303 name: author statement without traceable support used in manual assertion def: "A type of author statement without traceable support that is used in a manual assertion." [ECO:MCC] synonym: "NAS" EXACT [GOECO:NAS] synonym: "non-traceable author statement" RELATED [GOECO:NAS] xref: GOECO:NAS "non-traceable author statement" is_a: ECO:0000034 ! author statement without traceable support is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion intersection_of: ECO:0000034 ! author statement without traceable support intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "NAS" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/nas-non-traceable-author-statement xsd:string created_by: mchibucos creation_date: 2011-01-20T02:29:50Z [Term] id: ECO:0000304 name: author statement supported by traceable reference used in manual assertion def: "A type of author statement supported by traceable reference that is used in a manual assertion." [ECO:MCC] synonym: "TAS" EXACT [GOECO:TAS] synonym: "traceable author statement" RELATED [GOECO:TAS] xref: GOECO:TAS "traceable author statement" is_a: ECO:0000033 ! author statement supported by traceable reference is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion intersection_of: ECO:0000033 ! author statement supported by traceable reference intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "TAS" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/tas-traceable-author-statement xsd:string created_by: mchibucos creation_date: 2011-01-20T02:31:03Z [Term] id: ECO:0000305 name: curator inference used in manual assertion def: "A type of curator inference that is used in a manual assertion." [ECO:MCC] subset: valid_with_biological_process subset: valid_with_cellular_component subset: valid_with_molecular_function synonym: "IC" EXACT [GOECO:IC] synonym: "inferred by curator" EXACT [GOECO:IC] xref: GOECO:IC "inferred by curator" is_a: ECO:0000205 ! curator inference is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion intersection_of: ECO:0000205 ! curator inference intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IC" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ic-inferred-curator xsd:string created_by: mchibucos creation_date: 2011-01-20T02:33:32Z [Term] id: ECO:0000306 name: inference from background scientific knowledge used in manual assertion def: "A type of inference from background scientific knowledge that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000001 ! inference from background scientific knowledge is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion intersection_of: ECO:0000001 ! inference from background scientific knowledge intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IC" xsd:string created_by: mchibucos creation_date: 2011-01-20T02:35:11Z [Term] id: ECO:0000307 name: no evidence data found used in manual assertion def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an manual assertion." [ECO:MCC] synonym: "ND" EXACT [GOECO:ND] synonym: "no biological data available" EXACT [GOECO:ND] xref: GOECO:ND "no biological data available" is_a: ECO:0000035 ! no evidence data found is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion intersection_of: ECO:0000035 ! no evidence data found intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ND" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/nd-no-biological-data-available xsd:string created_by: mchibucos creation_date: 2011-01-20T02:43:18Z [Term] id: ECO:0000308 name: biological aspect of ancestor evidence def: "A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene." [ECO:MCC] comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2011-02-04T05:41:26Z [Term] id: ECO:0000309 name: cap analysis of gene expression evidence def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system." [ECO:MCC] comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. synonym: "CAGE" EXACT [PMID:16489339] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-02-17T01:33:09Z [Term] id: ECO:0000310 name: nano-cap analysis of gene expression evidence def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching." [ECO:MCC] comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA. synonym: "nanoCAGE" RELATED [PMID:20543846] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2011-02-17T01:33:34Z [Term] id: ECO:0000311 name: imported information def: "A type of documented statement evidence that is based on work performed by a person or group prior to a use by a different person or group." [ECO:MCC] is_a: ECO:0006151 ! documented statement evidence created_by: mchibucos creation_date: 2011-03-02T05:24:13Z [Term] id: ECO:0000312 name: imported information used in manual assertion def: "A type of imported information that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000311 ! imported information is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion intersection_of: ECO:0000311 ! imported information intersection_of: used_in ECO:0000218 ! manual assertion created_by: mchibucos creation_date: 2011-03-02T05:29:44Z [Term] id: ECO:0000313 name: imported information used in automatic assertion def: "A type of imported information that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000311 ! imported information is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion intersection_of: ECO:0000311 ! imported information intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2011-03-02T05:33:43Z [Term] id: ECO:0000314 name: direct assay evidence used in manual assertion def: "A type of direct assay evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity synonym: "IDA" EXACT [GOECO:IDA] synonym: "inferred from direct assay" EXACT [GOECO:IDA] xref: GOECO:IDA "inferred from direct assay" is_a: ECO:0000002 ! direct assay evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000002 ! direct assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ida-inferred-direct-assay xsd:string created_by: mchibucos creation_date: 2011-10-28T04:59:31Z [Term] id: ECO:0000315 name: mutant phenotype evidence used in manual assertion def: "A type of mutant phenotype evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IMP" EXACT [GOECO:IMP] synonym: "inferred from mutant phenotype" EXACT [GOECO:IMP] xref: GOECO:IMP "inferred from mutant phenotype" is_a: ECO:0000015 ! mutant phenotype evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000015 ! mutant phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/imp-inferred-mutant-phenotype xsd:string created_by: mchibucos creation_date: 2011-10-28T05:12:49Z [Term] id: ECO:0000316 name: genetic interaction evidence used in manual assertion def: "A type of genetic interaction experiment evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IGI" EXACT [GOECO:IGI] synonym: "inferred from genetic interaction" EXACT [GOECO:IGI] xref: GOECO:IGI "inferred from genetic interaction" is_a: ECO:0000011 ! genetic interaction evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000011 ! genetic interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/igi-inferred-genetic-interaction xsd:string created_by: mchibucos creation_date: 2011-10-28T05:14:42Z [Term] id: ECO:0000317 name: genomic context evidence used in manual assertion def: "A type of genomic context evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IGC" EXACT [GOECO:IGC] synonym: "inferred from genomic context" EXACT [GOECO:IGC] xref: GOECO:IGC "inferred from genomic context" is_a: ECO:0000177 ! genomic context evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000177 ! genomic context evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGC" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/igc-inferred-genomic-context xsd:string created_by: mchibucos creation_date: 2011-10-28T05:22:53Z [Term] id: ECO:0000318 name: biological aspect of ancestor evidence used in manual assertion def: "A type of biological aspect of ancestor evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IBA" EXACT [GOECO:IBA] synonym: "inferred from biological aspect of ancestor" EXACT [GOECO:IBA] xref: GOECO:IBA "inferred from biological aspect of ancestor" is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion is_a: ECO:0000308 ! biological aspect of ancestor evidence intersection_of: ECO:0000308 ! biological aspect of ancestor evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IBA" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/iba-inferred-biological-aspect-ancestor xsd:string created_by: mchibucos creation_date: 2011-10-28T05:23:51Z [Term] id: ECO:0000319 name: biological aspect of descendant evidence used in manual assertion def: "A type of biological aspect of descendant evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "IBD" EXACT [GOECO:IBD] synonym: "inferred from biological aspect of descendant" EXACT [GOECO:IBD] xref: GOECO:IBD "inferred from biological aspect of descendant" is_a: ECO:0000214 ! biological aspect of descendant evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000214 ! biological aspect of descendant evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IBD" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ibd-inferred-biological-aspect-descendent xsd:string created_by: mchibucos creation_date: 2011-10-28T05:25:09Z [Term] id: ECO:0000320 name: phylogenetic determination of loss of key residues evidence used in manual assertion def: "A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion." [ECO:MCC] synonym: "IKR" EXACT [GOECO:IKR] synonym: "IMR" EXACT [GOECO:IKR] synonym: "inferred from key residues" EXACT [GOECO:IKR] synonym: "inferred from missing residues" EXACT [GOECO:IKR] xref: GOECO:IKR "inferred from key residues" xref: GOECO:IMR "inferred from missing residues" is_a: ECO:0000216 ! phylogenetic determination of loss of key residues evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000216 ! phylogenetic determination of loss of key residues evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IKR" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ikr-inferred-key-residues xsd:string created_by: mchibucos creation_date: 2011-10-28T05:25:48Z [Term] id: ECO:0000321 name: rapid divergence from ancestral sequence evidence used in manual assertion def: "A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_gene subset: valid_with_protein synonym: "inferred from rapid divergence" EXACT [GOECO:IRD] synonym: "IRD" EXACT [GOECO:IRD] xref: GOECO:IRD "inferred from rapid divergence" is_a: ECO:0000215 ! rapid divergence from ancestral sequence evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000215 ! rapid divergence from ancestral sequence evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IRD" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ird-inferred-rapid-divergence xsd:string created_by: mchibucos creation_date: 2011-10-28T05:26:42Z [Term] id: ECO:0000322 name: imported manually asserted information used in automatic assertion def: "Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] is_a: ECO:0000313 ! imported information used in automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2011-12-13T12:04:48Z [Term] id: ECO:0000323 name: imported automatically asserted information used in automatic assertion def: "A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] is_a: ECO:0000313 ! imported information used in automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2011-12-13T02:06:25Z [Term] id: ECO:0000324 name: imaging assay evidence alt_id: ECO:0001122 alt_id: ECO:0005024 def: "A type of direct assay evidence in which an image is created and analyzed." [] synonym: "radiologic test evidence" NARROW [] xref: ECO:MCC is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000112 "In the images shown in Figure 9, the MelR focus is 15 x 33 nm across the shortest and longest axes of the oval in the complex with the shortened arm (right hand panel), compared to 9 x 12 nm in the other complex (centre panel). The simplest explanation for these observations, that is consistent with the location of sites in the DNA fragment (Figure 9), is that one type of complex contains 3 MelR molecules bound at sites 2, 1 and 1' (centre panel), whilst the other contains 4 MelR molecules bound at sites 2, 1, 1' and R (right panel)." xsd:string {xref="PMID:18346968"} created_by: mchibucos creation_date: 2012-02-23T12:01:43Z [Term] id: ECO:0000325 name: chromatography evidence def: "A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase." [ECO:MCC] synonym: "chromatographic evidence" EXACT [] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2012-05-16T11:46:27Z [Term] id: ECO:0000326 name: transcript splice pattern evidence def: "A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript." [ECO:MCC] is_a: ECO:0000200 ! sequence alignment evidence created_by: mchibucos creation_date: 2012-06-08T11:03:59Z [Term] id: ECO:0000327 name: whole transcript splice pattern evidence def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000326 ! transcript splice pattern evidence created_by: mchibucos creation_date: 2012-06-08T11:11:24Z [Term] id: ECO:0000328 name: coding sequence splice pattern evidence def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000326 ! transcript splice pattern evidence created_by: mchibucos creation_date: 2012-06-08T11:21:57Z [Term] id: ECO:0000329 name: whole transcript splice pattern evidence used in manual assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: mchibucos creation_date: 2012-06-08T11:40:03Z [Term] id: ECO:0000330 name: coding sequence splice pattern evidence used in manual assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: mchibucos creation_date: 2012-06-08T11:43:47Z [Term] id: ECO:0000331 name: coding sequence splice pattern evidence used in automatic assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. is_a: ECO:0000328 ! coding sequence splice pattern evidence is_a: ECO:0007825 {is_inferred="true"} ! transcript splice pattern evidence used in automatic assertion intersection_of: ECO:0000328 ! coding sequence splice pattern evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2012-06-08T11:48:09Z [Term] id: ECO:0000332 name: whole transcript splice pattern evidence used in automatic assertion def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion." [ECO:MCC] comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. is_a: ECO:0000327 ! whole transcript splice pattern evidence is_a: ECO:0007825 {is_inferred="true"} ! transcript splice pattern evidence used in automatic assertion intersection_of: ECO:0000327 ! whole transcript splice pattern evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2012-06-08T11:49:17Z [Term] id: ECO:0000333 name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence def: "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." [ECO:MCC, PubMed:4861258, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html] comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size. synonym: "SDS-PAGE evidence" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-07-31T02:56:43Z [Term] id: ECO:0000334 name: particle size and count assay evidence def: "A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined." [ECO:MCC] comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system. is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-07-31T03:19:32Z [Term] id: ECO:0000335 name: substance quantification evidence def: "A type of direct assay evidence where the quantity of a substance used as part of an assay is measured." [ECO:MCC] comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme. is_a: ECO:0000002 ! direct assay evidence created_by: mchibucos creation_date: 2012-07-31T03:52:46Z [Term] id: ECO:0000336 name: competitive growth assay evidence def: "A type of cell proliferation assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed usually by means of a fluorescent marker and flow cytometric analysis." [GOC:MAH, PMID:14718172, PMID:20537132] synonym: "fitness profiling" EXACT [GOC:MAH, PMID:20537132] is_a: ECO:0005007 ! cell proliferation assay evidence created_by: mchibucos creation_date: 2012-08-24T12:03:58Z [Term] id: ECO:0000337 name: gel electrophoresis evidence alt_id: ECO:0005026 def: "A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field." [ECO:MCC, wikipedia:Gel_electrophoresis] comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch. is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000112 "We analyzed the electrophoretic pattern of cytosolic proteins extracted from LM3 and LM3-2 cells, grown under standard conditions up to mid-exponential phase (Fig. 1). The presence of differentially expressed proteins in the two strains, together with results of previous studies [28,29] demonstrated the role of CcpA as global regulator in L. plantarum." xsd:string {xref="PMID:17129387"} created_by: mchibucos creation_date: 2012-08-24T12:11:51Z [Term] id: ECO:0000338 name: pulsed-field gel electrophoresis evidence def: "A type of gel electrophoresis evidence in which large DNA molecules are separated on agarose gel by alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous." [PMID:6373014] synonym: "PFGE" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-08-24T12:34:01Z [Term] id: ECO:0000339 name: two-dimensional agarose gel electrophoresis evidence def: "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." [PMID:16118435, PMID:8594382] comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape. synonym: "2-D agarose gel electrophoresis" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence created_by: mchibucos creation_date: 2012-08-24T12:42:37Z [Term] id: ECO:0000340 name: plasmid maintenance assay evidence def: "A type of experimental evidence in which a plasmid that is capable of being replicated is introduced into cells, cells are grown without selection for several generations, and retention of the plasmid is determined." [] {comment="ECO:MCC", comment="PMID:6323245"} comment: Loss per generation is determined by comparing colony numbers on selective and non-selective media. synonym: "minichromosome maintenance assay evidence" EXACT [PMID:6323245] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2012-08-24T01:27:58Z [Term] id: ECO:0000341 name: specific protein inhibition by antibody evidence def: "A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody." [ECO:MCC] is_a: ECO:0000020 ! protein inhibition evidence created_by: mchibucos creation_date: 2012-08-24T01:48:24Z [Term] id: ECO:0000342 name: support of intron positions by RNA-sequencing alignment evidence def: "A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence." [ECO:MCC] comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data. synonym: "Support of intron positions by RNA-seq alignment evidence" EXACT [] is_a: ECO:0000295 ! RNA-sequencing evidence created_by: mchibucos creation_date: 2013-04-03T04:22:05Z [Term] id: ECO:0000343 name: full support of intron positions by RNA-sequencing alignment evidence def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not. synonym: "Full support of intron positions by RNA-seq alignment evidence" EXACT [] is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence created_by: mchibucos creation_date: 2013-04-03T04:41:48Z [Term] id: ECO:0000344 name: partial support of intron positions by RNA-sequencing alignment evidence def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample. synonym: "Partial support of intron positions by RNA-seq alignment evidence" EXACT [] is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence created_by: mchibucos creation_date: 2013-04-03T04:50:46Z [Term] id: ECO:0000345 name: single exon transcript confirmation via alignment evidence def: "A type of sequence alignment evidence resulting from the comparison of a single exon transcript to a collection / database of known single exon genes." [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897596/] is_a: ECO:0000200 ! sequence alignment evidence created_by: mchibucos creation_date: 2013-04-03T05:10:34Z [Term] id: ECO:0000346 name: support of intron positions by RNA-sequencing alignment evidence used in manual assertion def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] synonym: "Support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion intersection_of: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:37:35Z [Term] id: ECO:0000347 name: support of intron positions by RNA-sequencing alignment evidence used in automatic assertion def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] synonym: "Support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion intersection_of: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:38:45Z [Term] id: ECO:0000348 name: full support of intron positions by RNA-sequencing alignment evidence used in automatic assertion def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] synonym: "Full support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion intersection_of: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:40:19Z [Term] id: ECO:0000349 name: full support of intron positions by RNA-sequencing alignment evidence used in manual assertion def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] synonym: "Full support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion intersection_of: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:41:34Z [Term] id: ECO:0000350 name: partial support of intron positions by RNA-sequencing alignment evidence used in automatic assertion def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] synonym: "Partial support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion intersection_of: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:42:53Z [Term] id: ECO:0000351 name: partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] synonym: "Partial support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion intersection_of: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: mchibucos creation_date: 2013-04-04T04:43:56Z [Term] id: ECO:0000352 name: evidence used in manual assertion def: "A type of evidence that is used in an manual assertion." [ECO:MCC] is_a: ECO:0000000 {is_inferred="true"} ! evidence intersection_of: ECO:0000000 ! evidence intersection_of: used_in ECO:0000218 ! manual assertion relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion created_by: mchibucos [Term] id: ECO:0000353 name: physical interaction evidence used in manual assertion def: "A type of physical interaction evidence that is used in a manual assertion." [ECO:MCC] subset: valid_with_chemical_entity subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "inferred from physical interaction" EXACT [GOECO:IPI] synonym: "IPI" EXACT [GOECO:IPI] xref: GOECO:IPI "inferred from physical interaction" is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000021 ! physical interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/ipi-inferred-physical-interaction xsd:string created_by: mchibucos creation_date: 2013-10-31T21:01:27Z [Term] id: ECO:0000354 name: gene neighbors evidence used in manual assertion def: "A type of gene neighbors evidence that is used in a manual assertion." [ECO:MCC] synonym: "ICL" RELATED [] synonym: "inferred from genome cluster" BROAD [] xref: GO_REF:0000025 "operon structure as IGC evidence" is_a: ECO:0000084 ! gene neighbors evidence is_a: ECO:0000317 {is_inferred="true"} ! genomic context evidence used in manual assertion intersection_of: ECO:0000084 ! gene neighbors evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGC" xsd:string created_by: mchibucos creation_date: 2013-11-19T13:37:24Z [Term] id: ECO:0000355 name: phylogenetic distribution evidence def: "A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony." [ECO:MCC, PMID:21238344] comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID:7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci. is_a: ECO:0000080 ! phylogenetic evidence created_by: mchibucos creation_date: 2014-04-22T17:02:31Z [Term] id: ECO:0000358 name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) def: "A type of transcript expression evidence resulting from a microarray analysis with the use of the Gene Set Enrichment Analysis (GSEA) method or the Fisher's-Exact (FE) method to assess the differential expression." [PMID:19725948] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:25:47Z [Term] id: ECO:0000359 name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) def: "A type of transcript expression evidence resulting from the use of the Gene Set Enrichment Analysis (GSEA) method or Fisher's-Exact (FE) method to assess an RNA-seq dataset for differential gene expression." [PMC:PMC2881125] is_a: ECO:0000009 ! transcript expression evidence created_by: mchibucos creation_date: 2014-09-26T16:26:48Z [Term] id: ECO:0000360 name: biological target-disease association via drug evidence def: "A type of experimental evidence resulting from the prediction of drug-target interactions by computational means." [https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2199-x] is_a: ECO:0000006 ! experimental evidence created_by: mchibucos creation_date: 2014-09-26T16:39:11Z [Term] id: ECO:0000361 name: inferential evidence def: "A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means." [ECO:MCC] comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true. is_a: ECO:0000000 ! evidence created_by: mchibucos creation_date: 2015-07-14T11:19:37Z [Term] id: ECO:0000362 name: computational inference def: "A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is \ncomputationally derived." [ECO:MCC, GOC:MC] synonym: "evidence based on computational logical inference" EXACT [] is_a: ECO:0000361 ! inferential evidence is_a: ECO:0007672 ! computational evidence created_by: mchibucos creation_date: 2015-07-14T11:24:27Z [Term] id: ECO:0000363 name: computational inference used in automatic assertion def: "A type of evidence based on computational logical inference that is used in automatic assertion." [ECO:MCC] synonym: "evidence based on computational logical inference used in automatic assertion" EXACT [] xref: GO_REF:0000108 "Automatic assignment of GO terms using logical inference, based on on inter-ontology links" is_a: ECO:0000362 ! computational inference is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion intersection_of: ECO:0000362 ! computational inference intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2015-07-14T11:35:08Z [Term] id: ECO:0000364 name: evidence based on logical inference from manual annotation used in automatic assertion def: "A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000363 ! computational inference used in automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2015-07-14T11:44:19Z [Term] id: ECO:0000366 name: evidence based on logical inference from automatic annotation used in automatic assertion def: "A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion." [ECO:MCC] is_a: ECO:0000363 ! computational inference used in automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: mchibucos creation_date: 2015-07-14T11:45:25Z [Term] id: ECO:0000501 name: evidence used in automatic assertion def: "A type of evidence that is used in an automatic assertion." [ECO:cjm] subset: valid_with_gene subset: valid_with_protein subset: valid_with_protein_complex synonym: "IEA" EXACT [GOECO:IEA] synonym: "inferred from electronic annotation" EXACT [GOECO:IEA] xref: GO_REF:0000003 "Gene Ontology annotation based on Enzyme Commission mapping." xref: GO_REF:0000004 "Gene Ontology annotation based on Swiss-Prot keyword mapping." xref: GO_REF:0000020 "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins." xref: GO_REF:0000041 "IEA (UniProt UniPathway)" xref: GO_REF:0000043 "Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping." xref: GO_REF:0000044 "Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt." xref: GO_REF:0000116 "Automatic Gene Ontology annotation based on Rhea mapping." xref: GOECO:IEA "inferred from electronic annotation" is_a: ECO:0000000 {is_inferred="true"} ! evidence intersection_of: ECO:0000000 ! evidence intersection_of: used_in ECO:0000203 ! automatic assertion relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string {comment="Default"} property_value: seeAlso http://geneontology.org/page/automatically-assigned-evidence-codes xsd:string created_by: cjm [Term] id: ECO:0001001 name: 3D cell culture evidence def: "A type of cell proliferation assay evidence resulting from the growth of cells in a micro-environment that mimics real tissue and establishes physiological cell-cell and cell-substrate interactions that regulate proliferation and differentiation." [PMID:21042962, url:http\://volttecnologia.com.br/wp-content/uploads/2016/03/Drug-discovery.pdf] is_a: ECO:0005007 ! cell proliferation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001002 name: [3H]arachidonic acid release assay evidence def: "A type of cell-based assay evidence resulting from labeling cells with [3H]arachidonic acid and subsequently determining the release of the arachidonic acid by the cells." [ISBN:0323152295, PMID:20693009] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001003 name: [3H]-thymidine incorporation assay evidence def: "A type of DNA synthesis cell proliferation assay evidence resulting from the measurement of cell proliferation rate by determining the incorporation of [3H]-thymidine into cellular nucleic acids." [OBI:0000669, url:http\://www.scientistsolutions.com/forum/cell-culture-and-tissue-culture-proliferationapoptosis/3h-thymidine-incorporation-assay-rat-1a] is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001004 name: 51Cr release assay evidence def: "A type of cytotoxicity assay evidence resulting from the measurement of the amount of radioactive 51Cr released in the supernatant of a sample of 51Cr pre-labeled cells via cytolysis by effector cells (cytotoxic T-cells)." [PMC:PMC3929704, url:http\://www4.mpbio.com/ecom/docs/proddata.nsf/5f64ffd4f38c2fda8525645d00769d68/53d2a75653615bab852568cb00572ff3?OpenDocument] is_a: ECO:0005012 ! cytotoxicity assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001005 name: 7-aminoactinomycin staining evidence def: "A type of apoptotic assay evidence resulting from the use of 7-aminoactinomycin D (7-AAD) to distinguish viable, apoptotic, and dead cells, using the fact that permeability of the cell membrane, and fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells." [ECO:RCT] synonym: "7-AAD staining evidence" EXACT [] synonym: "7-amino-actinomycin D staining evidence" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001006 name: adhesion assay evidence def: "A type of cell-based assay evidence resulting from the analysis of cell binding either to extracellular matrix proteins or other cells." [PMID:15576904, PMID:21909903] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001007 name: adoptive cell transfer evidence def: "A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host." [ECO:SN, url:http\://jco.ascopubs.org/content/23/10/2346.full.pdf+html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] synonym: "Adoptive immunotherapy" EXACT [] synonym: "passive immunization" EXACT [] is_a: ECO:0005502 ! ex vivo assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001008 name: alamarBlue assay evidence def: "A type of cell viability assay evidence resulting from the continuous measurement of fluorescence intensity from the viable cells, where a cell permeable compound, resazurin (the active ingredient of alamarBlue), is reduced to fluorescent resorufin." [PMC:PMC3478843, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/cell-profilteration-assay-protocols/cell-viability-with-alamarblue.html] is_a: ECO:0005004 ! cell viability assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001009 name: allograft transplantation phenotypic evidence def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation of an organ or tissue from one individual of the same species with a different genotype." [ECO:SN, url:http\://www.nature.com/subjects/allograft, url:http\://www.organdonor.gov/about/terms_and_topics/] synonym: "allograft transplantation experiment evidence" EXACT [] synonym: "allografting" EXACT [] synonym: "Allotransplantation" EXACT [] synonym: "tissue grafting" NARROW [] is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001010 name: anion-exchange chromatography evidence def: "A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules." [ECO:SN, PMID:20978968, url:http\://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography, url:http\://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/Ion%20Exchange%20Chromatography-1.pdf] is_a: ECO:0000325 ! chromatography evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001011 name: annexin-V staining evidence def: "A type of apoptotic assay evidence resulting from the addition and subsequent binding of Annexin V to phosphatidylserine in cells to detect if they are viable, apoptotic, or necrotic." [PMC:PMC3169266] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001012 name: cognitive assay phenotypic evidence def: "A type of experimental phenotypic evidence resulting from behavioral phenotyping and analysis to assess cognitive functioning." [PMID:24462904] synonym: "behavioral assay evidence" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001013 name: blocking monoclonal antibody evidence def: "A type of immunological assay evidence resulting from inhibitory effect of monoclonal antibody combining with an antigen over other antibodies." [PMID:11292349, url:http\://www.sciencedirect.com/science/article/pii/S0140673600034966] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001014 name: blocking peptide evidence def: "A type of immunological assay evidence resulting from the use of peptides to block antibodies from binding to their targets." [ECO:RCT] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001015 name: blocking polyclonal antibody evidence def: "A type of immunological assay evidence resulting from the inhibitory effect of polyclonal antibodies (antibodies secreted by different B cell lineages), which while not reacting with a specific antigen, serve to prevent other antibodies from doing so." [] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001016 name: blood test evidence def: "A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components." [ECO:SN, url:https\://www.nhlbi.nih.gov/health/health-topics/topics/bdt] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001017 name: Boyden chamber assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the number of cells that migrate from the upper compartment of a chamber (separated by a micro-porous membrane) to the lower compartment, in which chemotactic agents are present." [PMID:13872176, PMID:15576901] is_a: ECO:0005021 ! chemotaxis assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001018 name: bromodeoxyuridine incorporation assay evidence alt_id: ECO:0005010 def: "A type of nucleotide analog incorporation evidence resulting from the incorporation of bromodeoxyuridine (BrdU) as a thymidine analog into nuclear DNA, resulting in a label that can be tracked using antibody probes." [PMID:23690005] synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" EXACT [] synonym: "BrdU incorporation assay evidence" EXACT [] synonym: "BrdUrd incorporation assay evidence" EXACT [] synonym: "BUdR incorporation assay evidence" EXACT [] synonym: "cell proliferation marker detection assay evidence" BROAD [] is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001019 name: caspase assay evidence def: "A type of apoptotic assay evidence resulting from the detection of caspase activation, which results in the cleaving of intracellular substrates during apoptosis." [PMID:18314058, PMID:25086023] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001020 name: cell counting evidence def: "A type of cell-based assay evidence resulting from the quantification of a sample of cells." [ECO:RCT] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001021 name: cell permeability assay evidence def: "A type of cell-based assay evidence resulting from the quantification of cell permeability." [PMID:16962665] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001022 name: carboxyfluorescein diacetate succinimidyl ester staining evidence def: "A type of staining evidence resulting from monitoring division by the use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to label intracellular molecules with carboxyfluorescein; when a labeled cell divides, the number of carboxyfluorescein-tagged molecules is split." [PMC:PMC3185625] synonym: "CFDA-SE staining evidence" EXACT [] synonym: "CFSE-staining evidence" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001023 name: chemiluminescence-linked immunoassay evidence def: "A type of protein detection assay evidence resulting from a complex (a chemiluminescent compound, protein and a steroidal hapten) utilized as a labeled antigen, which emits light when treated with hydrogen peroxide and copper acetate at a high pH." [PMID:659901] synonym: "CLIA evidence" EXACT [] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001024 name: chimeric protein phenotypic evidence def: "A type of mutant phenotype evidence resulting from the fusion of two or more different genes to create one protein product." [PMID:22588898, PMID:25832756] synonym: "chimeric protein evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001025 name: co-electrophoresis evidence def: "A type of gel electrophoresis evidence resulting from the electrophoresis of two substances together, allowing for the characterization of ligand/nucleic acid binding interactions." [PMID:7668395] is_a: ECO:0000337 ! gel electrophoresis evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001026 name: co-localization evidence def: "A type of imaging assay evidence resulting from the limited observation of the co-occurrence of molecules in a subcellular location." [PMC:PMC3074624] is_a: ECO:0000324 ! imaging assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001027 name: colony counting evidence def: "A type of direct assay evidence resulting from the counting of microbial colonies that arise from viable cells grown on a plate or dish." [PMID:16558698, PMID:23457446] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001028 name: co-sedimentation assay evidence def: "A type of physical interaction evidence resulting from the separation of a mixture of molecules under the influence of a force such as artificial gravity, where molecules sedimenting together are assumed to interact." [MI:0027] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001029 name: comet assay evidence def: "A type of gel electrophoresis evidence resulting in the assessment of DNA breakage in a cell, based on size and shape of DNA migration." [OBI:0302736, PMID:10737956] is_a: ECO:0000337 ! gel electrophoresis evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001030 name: conditional knockout evidence def: "A type of knockout evidence resulting from a temporally-restricted and/or tissue-specific targeted gene removal." [PMC:PMC3572410] is_a: ECO:0001091 ! knockout phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001031 name: conditional knockin evidence def: "A type of knockin evidence resulting from the targeted introduction of a temporally-restricted and/or tissue-specific mutation." [PMID:16832820] is_a: ECO:0001090 ! knockin evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001032 name: constitutively active mutant evidence def: "A type of experimental phenotypic evidence resulting from a constitutively active mutant (CAM), resulting in mutant proteins that remain active in the absence of upstream signals." [PMID:12217490] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001033 name: cross-linking evidence def: "A type of protein binding evidence resulting from the identification of two interacting proteins (that exist in close proximity) by linking through covalent bonds followed by identification" [PMID:15530987, PMID:19241040] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000112 "To confirm this intriguing observation, we cross-linked AdpA and DNA in the presence of increasing amounts of ATP (figure 2b(iii)). In this assay, ATP prevented the formation of AdpA - DNA complexes in a concentration-dependent manner, an outcome consistent with the results obtained by SPR analysis." xsd:string {xref="PMID:22870392"} property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001034 name: crystallography evidence def: "A type of structure determination evidence resulting from the use of a narrow beam of x-rays to identify molecular structures by creating a diffraction pattern." [MI:0114] is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000112 "The crystal structures of E. coli MarR and Enterococcus faecalis SlyA-like proteins show their cross-sections are ~70 angstroms, suggesting they would protect ~20 bp of DNA (Alekshun et al., 2001; Wu et al., 2003). This suggests the presence of two SlyA dimers at the SlyA I site and at least three dimers at the SlyA II site." xsd:string {xref="PMID:17892462"} property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001035 name: cytochemistry evidence def: "A type of histochemistry evidence resulting from localizing chemical components of cells and organelles on histological sections." [PMID:11597006] is_a: ECO:0001050 ! histochemistry evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001036 name: cytochrome C release assay evidence def: "A type of apoptotic assay evidence resulting from the identification of an accumulation of cytochrome C in the cytoplasm after its release from the mitochondria, an early event in apoptosis." [PMID:10914021, PMID:12815469] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001037 name: 4',6-diamidino-2-phenylindole staining evidence def: "A type of staining evidence resulting from 4',6-diamidino-2-phenylindole (DAPI) binding to AT regions of DNA and emitting a blue fluorescence." [PMID:8580206, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols/dapi-imaging-protocol.html] synonym: "DAPI staining evidence" EXACT [] synonym: "diaminophenylindole staining" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001038 name: deletion mutation phenotypic evidence alt_id: ECO:0005510 def: "A type of mutant phenotype evidence resulting from a mutation in which there is the removal of one or more contiguous nucleotides that may result in altered function or other measurable properties." [SO:0000159] comment: As a child of 'experimental phenotypic evidence', this is not to be confused with the biological processes deletion mutation / deletion deficiency in which DNA is lost during DNA replication. comment: Contrast with knockout phenotypic evidence in which any number of changes to the DNA result in elimination of the function of the gene in question. comment: The length of the DNA deleted can range from a single nucleotide to enough material that could include multiple genes. synonym: "deletion mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000112 "In contrast, and in full agreement with the previous results of Wade et al. (17), the longer deletion in the TB23 fragment, that removes MelR binding site 2, results in a sharp reduction in the repression of the melR promoter by MelR." xsd:string {xref="PMID:18346968"} property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001039 name: DNA laddering assay evidence def: "A type of apoptotic assay evidence resulting from the visualization via gel electrophoresis of the fragmented DNA (DNA ladder) which is the result of apoptotic DNA fragmentation." [PMC:PMC4401164] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001040 name: RNA dot blot assay evidence def: "A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments." [ECO:SW, PMID:21424648] synonym: "RNA dot blot" RELATED [] is_a: ECO:0005518 ! RNA detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001042 name: dominant-negative mutant phenotypic evidence def: "A type of mutant phenotype evidence resulting from a mutated gene producing mutant polypeptides that disrupt the activity of the wild-type genes." [PMC:PMC2217636, PMID:8018332] synonym: "dominant-negative mutant evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001043 name: Edman degradation evidence used in manual assertion def: "A type of Edman degradation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001044 ! Edman degradation evidence intersection_of: ECO:0001044 ! Edman degradation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:59:00Z [Term] id: ECO:0001044 name: Edman degradation evidence def: "A type of protein detection assay evidence resulting from labeling a termonal amino acid residue and cleaving it from a peptide by sequentially removing one residue at a time from the amino end of the peptide, without disrupting the bonds." [doi:10.3891/acta.chem.scand.04-0283, OBI:0000705] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001045 name: eTag assay evidence def: "A type of affinity evidence resulting from the quantitative analysis of gene and protein expression monitored by proprietary eTag reporters." [PMID:15137949] is_a: ECO:0000023 ! affinity evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001046 name: filter binding assay evidence def: "A type of physical interaction evidence resulting from a mixture of two molecules passed through a nitrocellulose filter, where one may be immobilized on the filter, and if the immobilized molecule is capable of binding to the other, it will be retained on the filter as well." [PMID:23028069] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001047 name: fluorescence in situ hybridization evidence def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of fluorescent probes to detect complementary sequences of nucleic acids." [PMC:PMC346675, PMID:6812046] is_a: ECO:0001534 ! nucleic acid localization evidence is_a: ECO:0001836 ! in situ hybridization evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001048 name: fluorescence resonance energy transfer evidence def: "A type of dynamic fluorescence quenching evidence resulting from the measurement of the proximity of two fluorophores, where the energy from an excited molecular fluorophore to another fluorophore can only occur within ~10nm." [PMID:11516318, PMID:15696158] synonym: "FRET" EXACT [] is_a: ECO:0006273 ! dynamic fluorescence quenching evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001049 name: gel-filtration evidence def: "A type of chromatography evidence resulting from the exclusion of proteins based on molecular size by filtration through a porous matrix (swollen gel), from which larger molecules are excluded." [CHMO:0001011, doi:10.1038/nmeth0506-410] is_a: ECO:0000325 ! chromatography evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001050 name: histochemistry evidence def: "A type of histology evidence resulting from the identification of chemical components in cells and tissues." [MMO:0000497] is_a: ECO:0001051 ! histology evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001051 name: histology evidence def: "A type of imaging assay evidence resulting from the qualitative microscopic examination of cells or tissues." [OBI:0600020] is_a: ECO:0000324 ! imaging assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001052 name: high-performance liquid chromatography evidence def: "A type of chromatography evidence resulting from the separation of molecules in a solute by forcing the solvent containing the sample through a column packed with nonporous particles at high pressure." [PMID:16376355] synonym: "HPLC evidence" EXACT [] is_a: ECO:0000325 ! chromatography evidence property_value: IAO:0000112 "No OD increases with the mutant and the wild type were measured with DMSO provided as electron acceptor at 2 and 10 mM; however, HPLC analyses of cultures with 2 mM DMSO revealed that DMSO was completely consumed in wild type cultures, whereas no DMSO consumption was evident in the mutant cultures (Figure 3)." xsd:string {xref="PMID:21450087"} created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001053 name: immunocytochemistry evidence def: "A type of protein detection assay evidence resulting from the use of antibodies linked to coloring agents to localize structures in cell cultures to identify proteins." [MI:1200, OBI:0001986] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001054 name: immunodepletion evidence def: "A type of immunological assay evidence resulting from the use of antibodies to remove specific proteins from a sample." [BAO:0002505] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001055 name: immunohistochemistry evidence def: "A type of protein detection assay evidence resulting from the use of antibodies to detect proteins in localized cells of tissue sections." [OBI:0001986] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001056 name: induced mutation evidence def: "A type of genetic transformation evidence resulting from a mutation induced by a mutagenic compounds or irradiation." [OBI:0001154] comment: This term was made obsolete for a couple of reasons: the def. doesn’t make sense as the suggested means of mutagenesis fail to introduce exogenous DNA (it is a child of genetic transformation evidence) and the def. is too much like random mutagenesis evidence. property_value: IAO:0000234 SIB:PG xsd:string is_obsolete: true created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001057 name: in vitro acetylation assay evidence def: "A type of acetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0001546 ! acetylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001058 name: in vitro cleavage assay evidence def: "A type of cleavage assay evidence used in an in vitro experiment." [PMID:21121091] is_a: ECO:0001547 ! cleavage assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001059 name: in vitro deubiquitination assay evidence def: "A type of deubiquitination assay evidence used in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0001552 ! deubiquitination assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001060 name: in vitro deacetylation assay evidence def: "A type of deacetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0001548 ! deacetylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001061 name: in vitro defarnesylation assay evidence def: "A type of defarnesylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:16126733, SIB:PG] is_a: ECO:0001549 ! defarnesylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001062 name: in vitro demethylation assay evidence def: "A type of demethylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] is_a: ECO:0001550 ! demethylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001063 name: in vitro desumoylation assay evidence def: "A type of desumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0001551 ! desumoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001064 name: in vitro farnesylation assay evidence def: "A type of farnesylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] is_a: ECO:0001553 ! farnesylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001065 name: in vitro methylation assay evidence def: "A type of methylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] is_a: ECO:0001554 ! methylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001066 name: in vitro palmitoylation assay evidence def: "A type of palmitoylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:17077383, SIB:PG] is_a: ECO:0001555 ! palmitoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001067 name: in vitro phosphatase assay evidence def: "A type of phosphatase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] is_a: ECO:0001556 ! phosphatase assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001068 name: in vitro protein kinase assay evidence def: "A type of protein kinase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] is_a: ECO:0001558 ! protein kinase assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001069 name: in vitro polyADP-ribosylation assay evidence def: "A type of polyADP-ribosylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:21870253, SIB:PG] is_a: ECO:0001557 ! polyADP-ribosylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001070 name: in vitro sumoylation assay evidence def: "A type of sumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0001559 ! sumoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001071 name: in vitro transcription assay evidence def: "A type of transcription assay evidence used in an in vitro experiment." [ECO:SN, ECO:SW, PMID:21125481, SIB:PG] is_a: ECO:0000168 ! transcription assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001072 name: in vitro translation assay evidence def: "A type of translation assay evidence used in an in vitro experiment." [ECO:SN, PMID:18230759, SIB:PG] is_a: ECO:0001561 ! translation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001073 name: in vitro ubiquitination assay evidence def: "A type of ubiquitination assay evidenceused in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0001562 ! ubiquitination assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001074 name: in vivo acetylation assay evidence def: "A type of acetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0001546 ! acetylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001075 name: in vivo cleavage assay evidence def: "A type of cleavage assay evidence used in an in vivo experiment." [ECO:SN, PMID:22154596] is_a: ECO:0001547 ! cleavage assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001076 name: in vivo deacetylation assay evidence def: "A type of deacetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0001548 ! deacetylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001077 name: in vivo defarnesylation assay evidence def: "A type of defarnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:15556768, PMID:16507103, SIB:PG] is_a: ECO:0001549 ! defarnesylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001078 name: in vivo demethylation assay evidence def: "A type of demethylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] is_a: ECO:0001550 ! demethylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001079 name: in vivo deubiquitination assay evidence def: "A type of deubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0001552 ! deubiquitination assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001080 name: in vivo desumoylation assay evidence def: "A type of desumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0001551 ! desumoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001081 name: in vivo farnesylation assay evidence def: "A type of farnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:9030603, SIB:PG] is_a: ECO:0001553 ! farnesylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001082 name: in vivo methylation assay evidence def: "A type of methylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] is_a: ECO:0001554 ! methylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001083 name: in vivo palmitoylation assay evidence def: "A type of palmitoylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:10329400, SIB:PG] is_a: ECO:0001555 ! palmitoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001084 name: in vivo phosphatase assay evidence def: "A type of phosphatase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] is_a: ECO:0001556 ! phosphatase assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001085 name: in vivo protein kinase assay evidence def: "A type of protein kinase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] is_a: ECO:0001558 ! protein kinase assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001086 name: in vivo sumoylation assay evidence def: "A type of sumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0001559 ! sumoylation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001087 name: in vivo transcription assay evidence def: "A type of transcription assay evidence used in an in vivo experiment." [ECO:SN, PMID:12181418, SIB:PG] is_a: ECO:0000168 ! transcription assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001088 name: in vivo translation assay evidence def: "A type of translation assay evidence used in an in vivo experiment." [ECO:SN, PMID:24901308, SIB:PG] is_a: ECO:0001561 ! translation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001089 name: in vivo ubiquitination assay evidence def: "A type of ubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] is_a: ECO:0001562 ! ubiquitination assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001090 name: knockin evidence def: "A type of genetic transformation evidence resulting from the targetted replacement of a wild-type DNA sequence with a different sequence." [PMID:18077807, PMID:21800101] is_a: ECO:0005027 ! genetic transformation evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001091 name: knockout phenotypic evidence def: "A type of genetic transformation evidence resulting from a change in the DNA that results in the elimination of the function of the gene, allowing for functional analysis." [PMC:PMC2782548] comment: A change in the DNA may result from any number of processes such (deletion, insertion, frame shift, etc.) Additionally, the gene may be made inoperative via homologous recombination, site-specific nucleases, zinc finger nucleases, transcription activator-like effector nucleases (TALENs), or CRISPR (Clustered regularly interspaced short palindromic repeats). comment: Contrast with deletion mutation phenotypic evidence in which DNA is deleted which may result in altering the function of the resulting protein(s). synonym: "gene deletion evidence" NARROW [] synonym: "gene knockout evidence" NARROW [] synonym: "knockout evidence" EXACT [] is_a: ECO:0005027 ! genetic transformation evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001092 name: lipid binding assay evidence def: "A type of physical interaction evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to a lipid." [PMID:22848065, PMID:23681540] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001093 name: luminescence-based mammalian interactome mapping assay evidence def: "A type of physical interaction evidence resulting from the analysis of protein-protein interactions by use of a luciferase enzyme fused to a particular protein (or proteins), which are coexpressed with epitope-tagged partners in mammalian cells." [MI:0729, PMID:15761153] synonym: "LUMIER assay" EXACT [] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001094 name: macroscopy evidence def: "A type of experimental evidence that is visible to the naked eye cf. microscopy evidence which requires the aid of a microscope." [] is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001095 name: mammalian 2-hybrid assay evidence def: "A type of bait-prey hybrid interaction evidence resulting from the analysis of proteins of interest attached to two portions of the transcriptional activator, and in interaction bring the two portions together to increase expression of the reporter gene." [ECO:RCT, PMID:9043710] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001096 name: mass spectrometry evidence def: "A type of spectrometry evidence resulting from identifying the amount and type of material entities present in a sample by fragmenting it and measuring the mass-to-charge ratio of the resulting particles." [OBI:0000470] is_a: ECO:0005504 ! spectrometry evidence property_value: IAO:0000112 "The MS analyses showed that two VapB10 peaks (836.437, 1584.789 m/z) and three VapC10-His6 peaks (1049.526, 1299.733 and 1455.853 m/z) were detected in MS-DIGEST program, indicating that both VapB10 and VapC10-His6 had the expected peptide masses (Figure S3). These results suggest that VapB10 binds to VapC10-His6 forming the TA complex VapBC10 in vivo, which may cause the counteraction of the VapC10-induced growth arrest (Figure 2)." xsd:string {xref="PMID:24260461"} property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001097 name: medical imaging evidence def: "A type of imaging assay evidence resulting from the use of technology to visualize and provide information about the body to diagnose, treat, or monitor medical conditions." [url:http\://www.fda.gov/Radiation-EmittingProducts/RadiationEmittingProductsandProcedures/MedicalImaging/ucm2005914.htm] is_a: ECO:0000324 ! imaging assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001098 name: microscopy evidence def: "A type of direct assay evidence resulting from the use of a microscope." [ECO:MCC] comment: Microscopy may include any of the following: optical, electron, scanning probe, or X-ray. is_a: ECO:0000324 ! imaging assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001099 name: motility wound healing assay evidence alt_id: ECO:0001135 def: "A type of cell-based assay evidence to measure cell motility in which a \"wound\" is created in a cell monolayer and the migration of the cells is captured by imaging at regular intervals during cell migration to close the wound." [] synonym: "wound healing assay evidence" BROAD [] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001100 name: MTS assay evidence def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) in combination with phenazine methyl sulfate (PMS) as an intermediate electron acceptor reagent to produce a soluble formazan dye in viable cells." [NBK:144065, PMID:1867954] synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" NARROW [] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001101 name: MTT assay evidence def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble purple formazan dye in viable cells and measures cell viability / metabolic activity." [NBK:144065, PMID:6606682] synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" NARROW [] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001102 name: multiplex bead-based immunoassay evidence def: "A type of protein detection assay evidence resulting from determination of analyte concentration by fluorescent bead sets attached to an antibody (to a specific analyte) with a fluorescent reporter dye label attached to a second antibody (to a specific analyte)." [PMC:PMC1534009] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001103 name: natural variation mutant evidence def: "A type of experimental phenotypic evidence resulting from the observation of the impact of a natural mutation on the expressed traits." [ECO:RCT] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001104 name: nuclear fragmentation evidence def: "A type of apoptotic assay evidence resulting from the nuclei fragmenting into smaller pieces during programmed cell death." [PMID:16738861] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001105 name: nuclear magnetic resonance evidence def: "A type of magnetic resonance evidence in which atomic nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [url:https\://en.wikipedia.org/wiki/Nuclear_magnetic_resonance] is_a: ECO:0006252 ! magnetic resonance evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001106 name: nuclease protection assay evidence def: "A type of RNA detection assay evidence resulting from the detection and quantitation of specific RNAs (from a sample of total cellular RNA) that are bound by antisense RNA or DNA probes and therefore protected from nucleases." [PMID:17486122, PMID:18428580] is_a: ECO:0005518 ! RNA detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001107 name: nucleotide analog incorporation assay evidence def: "A type of DNA synthesis cell proliferation assay evidence resulting from the analysis and measurement of the incorporation of fluorescently labeled nucleotide analog(s) into the DNA." [PMC:PMC3149870] is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001108 name: phage display evidence def: "A type of protein binding evidence resulting from the panning of a phage library where the individual phage displayed a different peptide or protein by fusion to coat proteins on the capsid." [MI:0084, PMID:11680867] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001109 name: phosphoamino acid analysis evidence def: "A type of direct assay evidence resulting from the identification of the phosphorylated residue in a protein by amino acid analysis." [PMID:11680867, PMID:18429115] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001110 name: peptide affinity enrichment evidence def: "A type of affinity evidence resulting from the appending of affinity tags on proteins so that they may be purified from their biological source using an affinity technique." [] synonym: "phosphopeptide affinity enrichment evidence" NARROW [] is_a: ECO:0000023 ! affinity evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001111 name: physical examination evidence def: "A type of direct assay evidence resulting from the physical examination and measurement of the feature of a subject or sample." [ECO:RCT] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001112 name: peptide array evidence def: "A type of protein binding evidence resulting from protein-bound peptides detected from a collection of peptides arranged as an array and incubated with the partner protein in order to map protein-protein interaction sites." [PMID:21243154] synonym: "phosphopeptide array evidence" NARROW [] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001113 name: point mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from the change in a single nucleotide." [SO:1000008] synonym: "point mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001114 name: propidium iodide staining evidence def: "A type of staining evidence resulting from the binding and labeling of DNA (during apoptosis, when there is a loss of nuclear DNA content) with propidium iodide (PI) to identify the cells from which it originated." [PMID:17406435] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001115 name: fluorescence evidence def: "A type of direct assay evidence resulting from the analysis of a molecule by its intrinsic fluorescence, or by attaching it with a fluorophore." [url:http\://www.nature.com/subjects/biological-fluorescence] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001116 name: protein dot blot assay evidence def: "A type of protein detection assay evidence resulting from protein samples immobilized on a membrane, then subsequently incubated and imaged." [doi:10.1007/978-94-009-0951-9_24] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001117 name: protein microarray evidence def: "A type of protein detection assay evidence resulting from proteins immobilized to prepare protein chips, or microwells, that can be utilized to determine presence of proteins, monitor differential expression profiles, and/or study protein interactions." [PMC:PMC1828913] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001118 name: protein sequencing assay evidence def: "A type of sequencing assay evidence resulting from determining the sequence of amino acids in a protein." [ERO:0001287] is_a: ECO:0000220 ! sequencing assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001119 name: quantitative mass spectrometry evidence def: "A type of mass spectrometry evidence resulting from quantitative analysis of peptides, proteins, and proteomes." [PMID:22665296] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001120 name: radioisotope assay evidence def: "A type of direct assay evidence resulting from the assessment of biological reactions by use of a radioactive isotope to label the reactant." [ISBN:978-3-642-50036-7] synonym: "radioassay" EXACT [] synonym: "radioisotopic assay" EXACT [] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001121 name: radioimmunoassay evidence def: "A type of protein detection assay evidence resulting from the use of a radioligand to measure the binding of a substance to a specific antibody or other receptor system." [MeSH:D011863] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001123 name: resonant mirror biosensor evidence def: "A type of direct assay evidence from the analysis of the real-time interaction between an analyte in solution and its interactant linked to the surface of the resonant mirror." [PMID:19151938] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001124 name: restriction fragment detection evidence def: "A type of DNA detection assay evidence resulting from homologous DNA fragments digested by restriction enzymes, and the resulting restriction fragments are sorted by length to illustrate differences." [doi:10.1007/978-94-009-0951-9_24, url:http\://www.ncbi.nlm.nih.gov/probe/docs/techrflp/] is_a: ECO:0005519 ! DNA detection assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001126 name: spectrophotometry evidence def: "A type of spectrometry evidence resulting from the evaluation of a molecule in a fluid or solid by its ability to alter the transmission of light at specific wavelengths." [MMO:0000173] is_a: ECO:0005504 ! spectrometry evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001127 name: surface plasmon resonance evidence def: "A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction." [ECO:SW, PMID:11578932, PMID:19151937, PMID:8574707] comment: Plasmons are generated by an incident light beam. synonym: "SPR evidence" EXACT [PMID:11578932] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000112 "Electrophoretic mobility shift assay (EMSA) and surface plasmon resonance (SPR) demonstrated that AdpAHis6 specifically bound the oriC fragment (283-bp) containing the in silico-predicted A-boxes in a concentration-dependent manner (figures 1b and 2a), but not the remaining part of oriC (data not shown)." xsd:string {xref="PMID:22870392"} property_value: IAO:0000112 "Further confirmation of the specific interaction was obtained by conducting the competing surface plasmon resonance (SPR) assay with the unlabeled DNA fragments. As shown in Additional file 3, a significantly lower response was observed when either the unlabeled S2 or S5 was added together with MtrA, which indicated that they could compete the binding of MtrA with the promoter DNA on the chip." xsd:string {xref="PMID:20843371"} property_value: IAO:0000112 "Our SPR analysis revealed that also housekeeping genes required for ribosome function (rplR) and beta subunit RNA polymerase (rpoB) belong to the LexA regulon, a feature of the SOS network not yet observed in bacteria." xsd:string {xref="PMID:24713082"} property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001128 name: syngeneic transplantation experiment evidence def: "A type of experimental phenotypic evidence resulting from a transplantation in which the transplanted material (stem cells) is from an individual's identical twin." [PMID:18469352] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001129 name: TACE activity assay evidence def: "A type of enzymatic activity assay evidence resulting from the analysis of tumor necrosis factor (TNF)-a converting enzyme (TACE), which releases a soluble TNF-a from the membrane-bound precursor protein." [PMC:PMC1808921] synonym: "Tumor necrosis factor - alpha converting enzyme activity assay" EXACT [] is_a: ECO:0000005 ! enzymatic activity assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001130 name: tissue microarray evidence def: "A type of cytochemistry evidence resulting from the analysis of section(s) of a paraffin block containing embedded tissues cores arranged in an array pattern." [PMID:11770905] is_a: ECO:0001035 ! cytochemistry evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001131 name: transgenic organism evidence def: "A type of genetic transformation evidence resulting from an organism that has had its expressed phenotype altered by modification." [OBI:1000048] is_a: ECO:0005027 ! genetic transformation evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001132 name: tryptic phosphopeptide mapping assay evidence def: "A type of mass spectrometry evidence resulting from the analysis of prepared phosphopeptides separated in two dimensions on a TLC plate." [PMID:18429120] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001133 name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence def: "A type of apoptotic assay evidence resulting from the visualization of DNA fragmentation by detection of exposed 3'-OH ends, localized by terminal deoxynucleotidyl transferase (TdT) which then catalyzes the addition of labeled dUTPs." [PMID:22566045] synonym: "TdT-mediated dUTP-biotin nick end labeling assay" EXACT [] synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" EXACT [] synonym: "TUNEL assay evidence" EXACT [] is_a: ECO:0005034 ! apoptotic assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001134 name: urine test evidence def: "A type of direct assay evidence resulting from the analysis of a urine sample." [ECO:RCT] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001136 name: WST-1 assay evidence alt_id: ECO:0005009 def: "A type of cell proliferation assay evidence resulting from the assessment of metabolic activity from a water-soluble tetrazolium salt WST-1 being reduced outside the cell (by reacting with mitochondrial succinate-tetrazolium reductase) to form a formazan dye." [PMID:18417231] synonym: "metabolic cell proliferation assay evidence" BROAD [] is_a: ECO:0005007 ! cell proliferation assay evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001137 name: xenotransplantation phenotypic evidence def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation, implantation, or infusion of live cells, tissues, or organs between individuals of different species." [ECO:SN, url:http\://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html, url:http\://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/] synonym: "tissue grafting" NARROW [] synonym: "Xenograft transplantation" EXACT [] synonym: "Xenografting" EXACT [] synonym: "Xenotransplantation" EXACT [] synonym: "xenotransplantation experiment evidence" EXACT [] is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-16T11:30:54Z [Term] id: ECO:0001138 name: 3D cell culture evidence used in manual assertion def: "A type of 3D cell culture evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001001 ! 3D cell culture evidence is_a: ECO:0007696 {is_inferred="true"} ! cell proliferation assay evidence used in manual assertion intersection_of: ECO:0001001 ! 3D cell culture evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:18:35Z [Term] id: ECO:0001139 name: 51Cr release assay evidence used in manual assertion def: "A type of 51Cr release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001004 ! 51Cr release assay evidence is_a: ECO:0007702 {is_inferred="true"} ! cytotoxicity assay evidence used in manual assertion intersection_of: ECO:0001004 ! 51Cr release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:20:55Z [Term] id: ECO:0001140 name: 7-aminoactinomycin staining evidence used in manual assertion def: "A type of 7-aminoactinomycin staining evidence that is used in a manual assertion." [ECO:MCC] synonym: "7-AAD staining evidence" BROAD [] synonym: "7-amino-actinomycin D staining evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001005 ! 7-aminoactinomycin staining evidence intersection_of: ECO:0001005 ! 7-aminoactinomycin staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:22:01Z [Term] id: ECO:0001141 name: [3H]-thymidine incorporation assay evidence used in manual assertion def: "A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001003 ! [3H]-thymidine incorporation assay evidence is_a: ECO:0007697 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in manual assertion intersection_of: ECO:0001003 ! [3H]-thymidine incorporation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:31:52Z [Term] id: ECO:0001142 name: [3H]arachidonic acid release assay evidence used in manual assertion def: "A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001002 ! [3H]arachidonic acid release assay evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001002 ! [3H]arachidonic acid release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:32:44Z [Term] id: ECO:0001143 name: adhesion assay evidence used in manual assertion def: "A type of adhesion assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001006 ! adhesion assay evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001006 ! adhesion assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:33:38Z [Term] id: ECO:0001144 name: adoptive cell transfer evidence used in manual assertion def: "A type of adoptive cell transfer evidence that is used in a manual assertion." [ECO:MCC] synonym: "Adoptive immunotherapy" BROAD [] synonym: "passive immunization" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001007 ! adoptive cell transfer evidence intersection_of: ECO:0001007 ! adoptive cell transfer evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:36:19Z [Term] id: ECO:0001145 name: alamarBlue assay evidence used in manual assertion def: "A type of alamarBlue assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001008 ! alamarBlue assay evidence is_a: ECO:0007703 {is_inferred="true"} ! cell viability assay evidence used in manual assertion intersection_of: ECO:0001008 ! alamarBlue assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:37:21Z [Term] id: ECO:0001146 name: allograft transplantation phenotypic evidence used in manual assertion def: "A type of allograft transplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "allograft transplantation evidence used in manual assertion" EXACT [] synonym: "allografting" BROAD [] synonym: "Allotransplantation" BROAD [] synonym: "tissue grafting" RELATED [] is_a: ECO:0001009 ! allograft transplantation phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001009 ! allograft transplantation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:38:22Z [Term] id: ECO:0001147 name: anion-exchange chromatography evidence used in manual assertion def: "A type of anion-exchange chromatography evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001010 ! anion-exchange chromatography evidence is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion intersection_of: ECO:0001010 ! anion-exchange chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:44:30Z [Term] id: ECO:0001148 name: annexin-V staining evidence used in manual assertion def: "A type of annexin-V staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001011 ! annexin-V staining evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001011 ! annexin-V staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:52:01Z [Term] id: ECO:0001149 name: cognitive assay phenotypic evidence used in manual assertion def: "A type of cognitive assay phenotypic evidence used that is used in a manual assertion." [ECO:MCC] synonym: "behavioral assay evidence used in manual assertion" EXACT [] is_a: ECO:0001012 ! cognitive assay phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001012 ! cognitive assay phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:53:38Z [Term] id: ECO:0001150 name: blocking monoclonal antibody evidence used in manual assertion def: "A type of blocking monoclonal antibody evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001013 ! blocking monoclonal antibody evidence is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion intersection_of: ECO:0001013 ! blocking monoclonal antibody evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:54:44Z [Term] id: ECO:0001151 name: blocking peptide evidence used in manual assertion def: "A type of blocking peptide evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001014 ! blocking peptide evidence is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion intersection_of: ECO:0001014 ! blocking peptide evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T15:55:33Z [Term] id: ECO:0001152 name: blocking polyclonal antibody evidence used in manual assertion def: "A type of blocking polyclonal antibody evidence tthat is used in a manual assertion." [ECO:MCC] is_a: ECO:0001015 ! blocking polyclonal antibody evidence is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion intersection_of: ECO:0001015 ! blocking polyclonal antibody evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:01:39Z [Term] id: ECO:0001153 name: blood test evidence used in manual assertion def: "A type of blood test evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001016 ! blood test evidence intersection_of: ECO:0001016 ! blood test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:02:47Z [Term] id: ECO:0001154 name: Boyden chamber assay evidence used in manual assertion def: "A type of Boyden chamber assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001017 ! Boyden chamber assay evidence is_a: ECO:0007701 {is_inferred="true"} ! chemotaxis assay evidence used in manual assertion intersection_of: ECO:0001017 ! Boyden chamber assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:03:53Z [Term] id: ECO:0001155 name: bromodeoxyuridine incorporation assay evidence used in manual assertion def: "A type of bromodeoxyuridine incorporation assay evidence used in manual assertion." [ECO:MCC] synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" BROAD [] synonym: "BrdU incorporation assay evidence" BROAD [] synonym: "BrdUrd incorporation assay evidence" BROAD [] synonym: "BUdR incorporation assay evidence" BROAD [] is_a: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence is_a: ECO:0001241 {is_inferred="true"} ! nucleotide analog incorporation assay evidence used in manual assertion intersection_of: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:04:49Z [Term] id: ECO:0001156 name: caspase assay evidence used in manual assertion def: "A type of caspase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001019 ! caspase assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001019 ! caspase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:06:06Z [Term] id: ECO:0001157 name: cell counting evidence used in manual assertion def: "A type of cell counting evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001020 ! cell counting evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001020 ! cell counting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:07:13Z [Term] id: ECO:0001158 name: cell permeability assay evidence used in manual assertion def: "A type of cell permeability assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001021 ! cell permeability assay evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001021 ! cell permeability assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:07:58Z [Term] id: ECO:0001159 name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion." [ECO:MCC] synonym: "CFDA-SE staining evidence" BROAD [] synonym: "CFSE-staining evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence intersection_of: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:08:54Z [Term] id: ECO:0001160 name: chemiluminescence-linked immunoassay evidence used in manual assertion def: "A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion." [ECO:MCC] synonym: "CLIA evidence" BROAD [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001023 ! chemiluminescence-linked immunoassay evidence intersection_of: ECO:0001023 ! chemiluminescence-linked immunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:09:55Z [Term] id: ECO:0001161 name: chimeric protein phenotypic evidence used in manual assertion def: "A type of chimeric protein phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "chimeric protein evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0001024 ! chimeric protein phenotypic evidence intersection_of: ECO:0001024 ! chimeric protein phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:10:40Z [Term] id: ECO:0001162 name: co-electrophoresis evidence used in manual assertion def: "A type of co-electrophoresis evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001025 ! co-electrophoresis evidence intersection_of: ECO:0001025 ! co-electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:11:22Z [Term] id: ECO:0001163 name: co-localization evidence used in manual assertion def: "A type of co-localization evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001026 ! co-localization evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001026 ! co-localization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:12:04Z [Term] id: ECO:0001164 name: co-sedimentation assay evidence used in manual assertion def: "A type of co-sedimentation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001028 ! co-sedimentation assay evidence intersection_of: ECO:0001028 ! co-sedimentation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:12:55Z [Term] id: ECO:0001165 name: colony counting evidence used in manual assertion def: "A type of colony counting evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001027 ! colony counting evidence intersection_of: ECO:0001027 ! colony counting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:14:57Z [Term] id: ECO:0001166 name: comet assay evidence used in manual assertion def: "A type of comet assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001029 ! comet assay evidence intersection_of: ECO:0001029 ! comet assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:16:01Z [Term] id: ECO:0001167 name: conditional knockin evidence used in manual assertion def: "A type of conditional knockin evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001031 ! conditional knockin evidence is_a: ECO:0001224 {is_inferred="true"} ! knockin evidence used in manual assertion intersection_of: ECO:0001031 ! conditional knockin evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:17:22Z [Term] id: ECO:0001168 name: conditional knockout evidence used in manual assertion def: "A type of conditional knockout evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001030 ! conditional knockout evidence is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion intersection_of: ECO:0001030 ! conditional knockout evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:17:58Z [Term] id: ECO:0001169 name: constitutively active mutant evidence used in manual assertion def: "A type of constitutively active mutant evidence that is used a in manual assertion." [ECO:MCC] is_a: ECO:0001032 ! constitutively active mutant evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001032 ! constitutively active mutant evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:20:23Z [Term] id: ECO:0001170 name: cross-linking evidence used in manual assertion def: "A type of cross-linking evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001033 ! cross-linking evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion intersection_of: ECO:0001033 ! cross-linking evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:21:29Z [Term] id: ECO:0001171 name: crystallography evidence used in manual assertion def: "A type of crystallography evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001034 ! crystallography evidence intersection_of: ECO:0001034 ! crystallography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:22:20Z [Term] id: ECO:0001172 name: cytochemistry evidence used in manual assertion def: "A type of cytochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001035 ! cytochemistry evidence is_a: ECO:0001185 {is_inferred="true"} ! histochemistry evidence used in manual assertion intersection_of: ECO:0001035 ! cytochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:23:10Z [Term] id: ECO:0001173 name: cytochrome C release assay evidence used in manual assertion def: "A type of cytochrome C release assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001036 ! cytochrome C release assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001036 ! cytochrome C release assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:23:53Z [Term] id: ECO:0001174 name: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion." [ECO:MCC] synonym: "DAPI staining evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence intersection_of: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:25:03Z [Term] id: ECO:0001175 name: deletion mutation phenotypic evidence used in manual assertion def: "A type of deletion mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "deletion mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0001038 ! deletion mutation phenotypic evidence intersection_of: ECO:0001038 ! deletion mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:25:54Z [Term] id: ECO:0001176 name: DNA laddering assay evidence used in manual assertion def: "A type of DNA laddering assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001039 ! DNA laddering assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001039 ! DNA laddering assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:26:46Z [Term] id: ECO:0001177 name: RNA dot blot assay evidence used in manual assertion def: "A type of RNA dot blot assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "RNA dot blot evidence" BROAD [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001040 ! RNA dot blot assay evidence intersection_of: ECO:0001040 ! RNA dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:45:52Z [Term] id: ECO:0001179 name: dominant-negative mutant phenotypic evidence used in manual assertion def: "A type of dominant-negative mutant phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "dominant-negative mutant evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0001042 ! dominant-negative mutant phenotypic evidence intersection_of: ECO:0001042 ! dominant-negative mutant phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:47:44Z [Term] id: ECO:0001180 name: eTag assay evidence used in manual assertion def: "A type of eTag assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001045 ! eTag assay evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0001045 ! eTag assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T16:59:39Z [Term] id: ECO:0001181 name: filter binding assay evidence used in manual assertion def: "A type of filter binding assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001046 ! filter binding assay evidence intersection_of: ECO:0001046 ! filter binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:03:28Z [Term] id: ECO:0001182 name: fluorescence in situ hybridization evidence used in manual assertion def: "A type of fluorescence in situ hybridization evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001047 ! fluorescence in situ hybridization evidence is_a: ECO:0001838 {is_inferred="true"} ! in situ hybridization evidence used in manual assertion intersection_of: ECO:0001047 ! fluorescence in situ hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:04:20Z [Term] id: ECO:0001183 name: fluorescence resonance energy transfer evidence used in manual assertion def: "A type of fluorescence resonance energy transfer evidence that is used in a manual assertion." [ECO:MCC] synonym: "FRET evidence" BROAD [] is_a: ECO:0001048 ! fluorescence resonance energy transfer evidence is_a: ECO:0006327 {is_inferred="true"} ! dynamic fluorescence quenching evidence used in manual assertion intersection_of: ECO:0001048 ! fluorescence resonance energy transfer evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:05:22Z [Term] id: ECO:0001184 name: gel-filtration evidence used in manual assertion def: "A type of gel-filtration evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001049 ! gel-filtration evidence is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion intersection_of: ECO:0001049 ! gel-filtration evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:06:14Z [Term] id: ECO:0001185 name: histochemistry evidence used in manual assertion def: "A type of histochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001050 ! histochemistry evidence is_a: ECO:0001187 {is_inferred="true"} ! histology evidence used in manual assertion intersection_of: ECO:0001050 ! histochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:07:19Z [Term] id: ECO:0001186 name: immunocytochemistry evidence used in manual assertion def: "A type of immunocytochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001053 ! immunocytochemistry evidence intersection_of: ECO:0001053 ! immunocytochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:12:39Z [Term] id: ECO:0001187 name: histology evidence used in manual assertion def: "A type of histology evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001051 ! histology evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001051 ! histology evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:09:50Z [Term] id: ECO:0001188 name: immunodepletion evidence used in manual assertion def: "A type of immunodepletion evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001054 ! immunodepletion evidence is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion intersection_of: ECO:0001054 ! immunodepletion evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:16:19Z [Term] id: ECO:0001189 name: immunohistochemistry evidence used in manual assertion def: "A type of immunohistochemistry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001055 ! immunohistochemistry evidence intersection_of: ECO:0001055 ! immunohistochemistry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:17:22Z [Term] id: ECO:0001190 name: in vitro acetylation assay evidence used in manual assertion def: "A type of in vitro acetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001057 ! in vitro acetylation assay evidence intersection_of: ECO:0001057 ! in vitro acetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:19:24Z [Term] id: ECO:0001191 name: in vitro cleavage assay evidence used in manual assertion def: "A type of in vitro cleavage assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001058 ! in vitro cleavage assay evidence intersection_of: ECO:0001058 ! in vitro cleavage assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:21:22Z [Term] id: ECO:0001192 name: in vitro deacetylation assay evidence used in manual assertion def: "A type of in vitro deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001060 ! in vitro deacetylation assay evidence intersection_of: ECO:0001060 ! in vitro deacetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:23:42Z [Term] id: ECO:0001193 name: in vitro defarnesylation assay evidence used in manual assertion def: "A type of in vitro defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001061 ! in vitro defarnesylation assay evidence intersection_of: ECO:0001061 ! in vitro defarnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:25:45Z [Term] id: ECO:0001194 name: in vitro demethylation assay evidence used in manual assertion def: "A type of in vitro demethylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001062 ! in vitro demethylation assay evidence intersection_of: ECO:0001062 ! in vitro demethylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:27:00Z [Term] id: ECO:0001195 name: in vitro desumoylation assay evidence used in manual assertion def: "A type of in vitro desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001063 ! in vitro desumoylation assay evidence intersection_of: ECO:0001063 ! in vitro desumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:29:01Z [Term] id: ECO:0001196 name: in vitro deubiquitination assay evidence used in manual assertion def: "A type of in vitro deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001059 ! in vitro deubiquitination assay evidence intersection_of: ECO:0001059 ! in vitro deubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:29:48Z [Term] id: ECO:0001197 name: in vitro farnesylation assay evidence used in manual assertion def: "A type of in vitro farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001064 ! in vitro farnesylation assay evidence intersection_of: ECO:0001064 ! in vitro farnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:30:50Z [Term] id: ECO:0001198 name: in vitro methylation assay evidence used in manual assertion def: "A type of in vitro methylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001065 ! in vitro methylation assay evidence intersection_of: ECO:0001065 ! in vitro methylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:31:54Z [Term] id: ECO:0001199 name: in vitro palmitoylation assay evidence used in manual assertion def: "A type of in vitro palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001066 ! in vitro palmitoylation assay evidence intersection_of: ECO:0001066 ! in vitro palmitoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:32:57Z [Term] id: ECO:0001200 name: in vitro phosphatase assay evidence used in manual assertion def: "A type of in vitro phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001067 ! in vitro phosphatase assay evidence intersection_of: ECO:0001067 ! in vitro phosphatase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:34:16Z [Term] id: ECO:0001201 name: in vitro polyADP-ribosylation assay evidence used in manual assertion def: "A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence intersection_of: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:34:57Z [Term] id: ECO:0001202 name: in vitro protein kinase assay evidence used in manual assertion def: "A type of in vitro protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001068 ! in vitro protein kinase assay evidence is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion intersection_of: ECO:0001068 ! in vitro protein kinase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:39:57Z [Term] id: ECO:0001203 name: in vitro sumoylation assay evidence used in manual assertion def: "A type of in vitro sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001070 ! in vitro sumoylation assay evidence intersection_of: ECO:0001070 ! in vitro sumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:41:25Z [Term] id: ECO:0001204 name: in vitro transcription assay evidence used in manual assertion def: "A type of in vitro transcription assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001071 ! in vitro transcription assay evidence intersection_of: ECO:0001071 ! in vitro transcription assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:44:40Z [Term] id: ECO:0001205 name: in vitro translation assay evidence used in manual assertion def: "A type of in vitro translation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001072 ! in vitro translation assay evidence intersection_of: ECO:0001072 ! in vitro translation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:46:30Z [Term] id: ECO:0001206 name: in vitro ubiquitination assay evidence used in manual assertion def: "A type of in vitro ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001073 ! in vitro ubiquitination assay evidence intersection_of: ECO:0001073 ! in vitro ubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:47:18Z [Term] id: ECO:0001207 name: in vivo acetylation assay evidence used in manual assertion def: "A type of in vivo acetylation assay evidence that is used in manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001074 ! in vivo acetylation assay evidence intersection_of: ECO:0001074 ! in vivo acetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:48:56Z [Term] id: ECO:0001208 name: in vivo cleavage assay evidence used in manual assertion def: "A type of in vivo cleavage assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001075 ! in vivo cleavage assay evidence intersection_of: ECO:0001075 ! in vivo cleavage assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:49:43Z [Term] id: ECO:0001209 name: in vivo deacetylation assay evidence used in manual assertion def: "A type of in vivo deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001076 ! in vivo deacetylation assay evidence intersection_of: ECO:0001076 ! in vivo deacetylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:50:47Z [Term] id: ECO:0001210 name: in vivo defarnesylation assay evidence used in manual assertion def: "A type of in vivo defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001077 ! in vivo defarnesylation assay evidence intersection_of: ECO:0001077 ! in vivo defarnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:52:41Z [Term] id: ECO:0001211 name: in vivo demethylation assay evidence used in manual assertion def: "A type of in vivo demethylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001078 ! in vivo demethylation assay evidence intersection_of: ECO:0001078 ! in vivo demethylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:53:40Z [Term] id: ECO:0001212 name: in vivo desumoylation assay evidence used in manual assertion def: "A type of in vivo desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001080 ! in vivo desumoylation assay evidence intersection_of: ECO:0001080 ! in vivo desumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:54:36Z [Term] id: ECO:0001213 name: in vivo deubiquitination assay evidence used in manual assertion def: "A type of in vivo deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001079 ! in vivo deubiquitination assay evidence intersection_of: ECO:0001079 ! in vivo deubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:56:28Z [Term] id: ECO:0001214 name: in vivo farnesylation assay evidence used in manual assertion def: "A type of in vivo farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001081 ! in vivo farnesylation assay evidence intersection_of: ECO:0001081 ! in vivo farnesylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T17:57:18Z [Term] id: ECO:0001215 name: in vivo methylation assay evidence used in manual assertion def: "A type of in vivo methylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001082 ! in vivo methylation assay evidence intersection_of: ECO:0001082 ! in vivo methylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:03:16Z [Term] id: ECO:0001216 name: in vivo palmitoylation assay evidence used in manual assertion def: "A type of in vivo palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001083 ! in vivo palmitoylation assay evidence intersection_of: ECO:0001083 ! in vivo palmitoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:04:07Z [Term] id: ECO:0001217 name: in vivo phosphatase assay evidence used in manual assertion def: "A type of in vivo phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001084 ! in vivo phosphatase assay evidence intersection_of: ECO:0001084 ! in vivo phosphatase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:06:08Z [Term] id: ECO:0001218 name: in vivo protein kinase assay evidence used in manual assertion def: "A type of in vivo protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001085 ! in vivo protein kinase assay evidence is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion intersection_of: ECO:0001085 ! in vivo protein kinase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:07:58Z [Term] id: ECO:0001219 name: in vivo sumoylation assay evidence used in manual assertion def: "A type of in vivo sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001086 ! in vivo sumoylation assay evidence intersection_of: ECO:0001086 ! in vivo sumoylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:09:49Z [Term] id: ECO:0001220 name: in vivo transcription assay evidence used in manual assertion def: "A type of in vivo transcription assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001087 ! in vivo transcription assay evidence intersection_of: ECO:0001087 ! in vivo transcription assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:11:20Z [Term] id: ECO:0001221 name: in vivo translation assay evidence used in manual assertion def: "A type of in vivo translation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001088 ! in vivo translation assay evidence intersection_of: ECO:0001088 ! in vivo translation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:42:50Z [Term] id: ECO:0001222 name: in vivo ubiquitination assay evidence used in manual assertion def: "A type of in vivo ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001089 ! in vivo ubiquitination assay evidence intersection_of: ECO:0001089 ! in vivo ubiquitination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:44:11Z [Term] id: ECO:0001223 name: induced mutation evidence used in manual assertion def: "A type of induced mutation evidence that is used in a manual assertion." [ECO:MCC] property_value: IAO:0000234 SIB:PG xsd:string is_obsolete: true created_by: mchibucos creation_date: 2014-03-17T18:45:56Z [Term] id: ECO:0001224 name: knockin evidence used in manual assertion def: "A type of knockin evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001090 ! knockin evidence is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion intersection_of: ECO:0001090 ! knockin evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:49:35Z [Term] id: ECO:0001225 name: knockout evidence used in manual assertion def: "A type of knockout evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001091 ! knockout phenotypic evidence is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion intersection_of: ECO:0001091 ! knockout phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:51:31Z [Term] id: ECO:0001226 name: lipid binding assay evidence used in manual assertion def: "A type of lipid binding assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001092 ! lipid binding assay evidence intersection_of: ECO:0001092 ! lipid binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:52:19Z [Term] id: ECO:0001227 name: luminescence-based mammalian interactome mapping assay evidence used in manual assertion def: "A type of LUMIER assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "LUMIER assay evidence" BROAD [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence intersection_of: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:55:16Z [Term] id: ECO:0001228 name: macroscopy evidence used in manual assertion def: "A type of macroscopy evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001094 ! macroscopy evidence intersection_of: ECO:0001094 ! macroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:56:21Z [Term] id: ECO:0001229 name: mammalian 2-hybrid assay evidence used in manual assertion def: "A type of mammalian 2-hybrid assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001095 ! mammalian 2-hybrid assay evidence is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion intersection_of: ECO:0001095 ! mammalian 2-hybrid assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:57:25Z [Term] id: ECO:0001230 name: mass spectrometry evidence used in manual assertion def: "A type of mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001096 ! mass spectrometry evidence intersection_of: ECO:0001096 ! mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T18:59:50Z [Term] id: ECO:0001231 name: medical imaging evidence used in manual assertion def: "A type of medical imaging evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001097 ! medical imaging evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001097 ! medical imaging evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:04:43Z [Term] id: ECO:0001232 name: microscopy evidence used in manual assertion def: "A type of microscopy evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion intersection_of: ECO:0001098 ! microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:05:37Z [Term] id: ECO:0001233 name: motility wound healing assay evidence used in manual assertion alt_id: ECO:0001262 def: "A type of motility wound healing assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "wound healing assay evidence used in manual assertion" BROAD [] is_a: ECO:0001099 ! motility wound healing assay evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001099 ! motility wound healing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:08:13Z [Term] id: ECO:0001234 name: MTS assay evidence used in manual assertion def: "A type of MTS assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" RELATED [] is_a: ECO:0001100 ! MTS assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001100 ! MTS assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:08:51Z [Term] id: ECO:0001235 name: MTT assay evidence used in manual assertion def: "A type of MTT assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" RELATED [] is_a: ECO:0001101 ! MTT assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001101 ! MTT assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:13:17Z [Term] id: ECO:0001236 name: multiplex bead-based immunoassay evidence used in manual assertion def: "A type of multiplex bead-based immunoassay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001102 ! multiplex bead-based immunoassay evidence intersection_of: ECO:0001102 ! multiplex bead-based immunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:14:03Z [Term] id: ECO:0001237 name: natural variation mutant evidence used in manual assertion def: "A type of natural variation mutant evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001103 ! natural variation mutant evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001103 ! natural variation mutant evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:15:48Z [Term] id: ECO:0001238 name: nuclear magnetic resonance evidence used in manual assertion def: "A type of nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001105 ! nuclear magnetic resonance evidence is_a: ECO:0006285 {is_inferred="true"} ! magnetic resonance evidence used in manual assertion intersection_of: ECO:0001105 ! nuclear magnetic resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:18:50Z [Term] id: ECO:0001239 name: nuclear fragmentation evidence used in manual assertion def: "A type of nuclear fragmentation evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001104 ! nuclear fragmentation evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001104 ! nuclear fragmentation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:19:55Z [Term] id: ECO:0001240 name: nuclease protection assay evidence used in manual assertion def: "A type of nuclease protection assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001106 ! nuclease protection assay evidence intersection_of: ECO:0001106 ! nuclease protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:23:41Z [Term] id: ECO:0001241 name: nucleotide analog incorporation assay evidence used in manual assertion def: "A type of nucleotide analog incorporation assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence is_a: ECO:0007697 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in manual assertion intersection_of: ECO:0001107 ! nucleotide analog incorporation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:25:17Z [Term] id: ECO:0001242 name: phage display evidence used in manual assertion def: "A type of phage display evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001108 ! phage display evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion intersection_of: ECO:0001108 ! phage display evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:27:17Z [Term] id: ECO:0001243 name: phosphoamino acid analysis evidence used in manual assertion def: "A type of phosphoamino acid analysis evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001109 ! phosphoamino acid analysis evidence intersection_of: ECO:0001109 ! phosphoamino acid analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:28:59Z [Term] id: ECO:0001244 name: peptide affinity enrichment evidence used in manual assertion def: "A type of peptide affinity enrichment evidence that is used in a manual assertion." [ECO:MCC] synonym: "phosphopeptide affinity enrichment evidence" RELATED [] is_a: ECO:0001110 ! peptide affinity enrichment evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0001110 ! peptide affinity enrichment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T19:30:13Z [Term] id: ECO:0001245 name: peptide array evidence used in manual assertion def: "A type of peptide array evidence that is used in a manual assertion." [ECO:MCC] synonym: "phosphopeptide array evidence" RELATED [] is_a: ECO:0001112 ! peptide array evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion intersection_of: ECO:0001112 ! peptide array evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:16:45Z [Term] id: ECO:0001246 name: physical examination evidence used in manual assertion def: "A type of physical examination evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001111 ! physical examination evidence intersection_of: ECO:0001111 ! physical examination evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:17:27Z [Term] id: ECO:0001247 name: point mutation phenotypic evidence used in manual assertion def: "A type of point mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "point mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0001113 ! point mutation phenotypic evidence intersection_of: ECO:0001113 ! point mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:18:10Z [Term] id: ECO:0001248 name: propidium iodide staining evidence used in manual assertion def: "A type of propidium iodide staining evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001114 ! propidium iodide staining evidence intersection_of: ECO:0001114 ! propidium iodide staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:18:58Z [Term] id: ECO:0001249 name: fluorescence evidence used in manual assertion def: "A type of protein detection by fluorescence evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001115 ! fluorescence evidence intersection_of: ECO:0001115 ! fluorescence evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:19:35Z [Term] id: ECO:0001250 name: protein dot blot assay evidence used in manual assertion def: "A type of protein dot blot assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001116 ! protein dot blot assay evidence intersection_of: ECO:0001116 ! protein dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:20:30Z [Term] id: ECO:0001251 name: protein microarray evidence used in manual assertion def: "A type of protein microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001117 ! protein microarray evidence intersection_of: ECO:0001117 ! protein microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:21:11Z [Term] id: ECO:0001252 name: protein sequencing assay evidence used in manual assertion def: "A type of protein sequencing assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001118 ! protein sequencing assay evidence intersection_of: ECO:0001118 ! protein sequencing assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:22:08Z [Term] id: ECO:0001253 name: quantitative mass spectrometry evidence used in manual assertion def: "A type of quantitative mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001119 ! quantitative mass spectrometry evidence is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion intersection_of: ECO:0001119 ! quantitative mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:23:15Z [Term] id: ECO:0001254 name: radioisotope assay evidence used in manual assertion def: "A type of radioisotope assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "radioassay evidence" BROAD [] synonym: "radioisotopic assay evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001120 ! radioisotope assay evidence intersection_of: ECO:0001120 ! radioisotope assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:27:05Z [Term] id: ECO:0001255 name: radioimmunoassay evidence used in manual assertion def: "A type of radioimmunoassay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001121 ! radioimmunoassay evidence intersection_of: ECO:0001121 ! radioimmunoassay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:28:04Z [Term] id: ECO:0001256 name: imaging assay evidence used in manual assertion def: "A type of imaging assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "radiologic test evidence" RELATED [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000324 ! imaging assay evidence intersection_of: ECO:0000324 ! imaging assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:00Z [Term] id: ECO:0001257 name: restriction fragment detection evidence used in manual assertion def: "A type of restriction fragment detection evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001124 ! restriction fragment detection evidence intersection_of: ECO:0001124 ! restriction fragment detection evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:05Z [Term] id: ECO:0001258 name: spectrophotometry evidence used in manual assertion def: "A type of spectrophotometry evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001126 ! spectrophotometry evidence intersection_of: ECO:0001126 ! spectrophotometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:09Z [Term] id: ECO:0001259 name: syngeneic transplantation experiment evidence used in manual assertion def: "A type of syngeneic transplantation experiment evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001128 ! syngeneic transplantation experiment evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001128 ! syngeneic transplantation experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:13Z [Term] id: ECO:0001260 name: xenotransplantation phenotypic evidence used in manual assertion def: "A type of xenotransplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] synonym: "tissue grafting" RELATED [] synonym: "xenograft transplantation evidence" BROAD [] synonym: "xenografting evidence" BROAD [] synonym: "xenotransplantation evidence" BROAD [] synonym: "xenotransplantation experiment evidence used in manual assertion" EXACT [] is_a: ECO:0001137 ! xenotransplantation phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0001137 ! xenotransplantation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:18Z [Term] id: ECO:0001261 name: WST-1 assay evidence used in manual assertion def: "A type of WST-1 assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "metabolic cell proliferation assay evidence" BROAD [] is_a: ECO:0001136 ! WST-1 assay evidence is_a: ECO:0007696 {is_inferred="true"} ! cell proliferation assay evidence used in manual assertion intersection_of: ECO:0001136 ! WST-1 assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:22Z [Term] id: ECO:0001263 name: urine test evidence used in manual assertion def: "A type of urine test evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001134 ! urine test evidence intersection_of: ECO:0001134 ! urine test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:30Z [Term] id: ECO:0001264 name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "TdT-mediated dUTP-biotin nick end labeling assay evidence" BROAD [] synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" BROAD [] synonym: "TUNEL assay evidence" BROAD [] is_a: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence is_a: ECO:0007698 {is_inferred="true"} ! apoptotic assay evidence used in manual assertion intersection_of: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:34Z [Term] id: ECO:0001265 name: tryptic phosphopeptide mapping assay evidence used in manual assertion def: "A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion intersection_of: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:38Z [Term] id: ECO:0001266 name: transgenic organism evidence used in manual assertion def: "A type of transgenic organism evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001131 ! transgenic organism evidence is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion intersection_of: ECO:0001131 ! transgenic organism evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:41Z [Term] id: ECO:0001267 name: tissue microarray evidence used in manual assertion def: "A type of tissue microarray evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0001130 ! tissue microarray evidence is_a: ECO:0001172 {is_inferred="true"} ! cytochemistry evidence used in manual assertion intersection_of: ECO:0001130 ! tissue microarray evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:45Z [Term] id: ECO:0001268 name: TACE activity assay evidence used in manual assertion def: "A type of TACE activity assay evidence that is used in a manual assertion." [ECO:MCC] synonym: "Tumor necrosis factor - alpha converting enzyme activity assay evidence" BROAD [] is_a: ECO:0001129 ! TACE activity assay evidence is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion intersection_of: ECO:0001129 ! TACE activity assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:49Z [Term] id: ECO:0001269 name: surface plasmon resonance evidence used in manual assertion def: "A type of surface plasmon resonance evidence that is used in a manual assertion." [ECO:MCC] synonym: "SPR evidence" BROAD [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001127 ! surface plasmon resonance evidence intersection_of: ECO:0001127 ! surface plasmon resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:54Z [Term] id: ECO:0001270 name: restriction landmark genomic scanning evidence used in manual assertion def: "A type of restriction landmark genome scanning evidence that is used in a manual assertion." [ECO:MCC] synonym: "restriction landmark genome scanning evidence" BROAD [] synonym: "RLGS evidence" BROAD [] is_a: ECO:0000089 ! restriction landmark genomic scanning evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000089 ! restriction landmark genomic scanning evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:29:58Z [Term] id: ECO:0001271 name: resonant mirror biosensor evidence used in manual assertion def: "A type of resonant mirror biosensor evidence that is used in a manual assertion." [ECO:MCC] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001123 ! resonant mirror biosensor evidence intersection_of: ECO:0001123 ! resonant mirror biosensor evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T20:30:02Z [Term] id: ECO:0001272 name: high-performance liquid chromatography evidence used in manual assertion def: "A type of high-performance liquid chromatography evidence that is used in a manual assertion." [ECO:MCC] synonym: "HPLC evidence" BROAD [] is_a: ECO:0001052 ! high-performance liquid chromatography evidence is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion intersection_of: ECO:0001052 ! high-performance liquid chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: mchibucos creation_date: 2014-03-16T20:49:54Z [Term] id: ECO:0001273 name: ectopic expression evidence used in manual assertion def: "A type of ectopic expression evidence that is used in a manual assertion." [ECO:MCC] synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0000017 ! ectopic expression evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000017 ! ectopic expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: mchibucos creation_date: 2014-03-17T22:34:56Z [Term] id: ECO:0001522 name: small molecule detection assay evidence def: "A type of molecule detection assay evidence resulting from the detection and quantification of a small molecule (a low molecular weight (typically < 900 daltons) organic compound), such as lipids, sugars, animo acids, drugs, etc." [] synonym: "metabolite detection assay evidence" NARROW [] is_a: ECO:0005516 ! molecule detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro [Term] id: ECO:0001533 name: localization evidence def: "A type of direct assay evidence in which the sub-cellular location of a protein or nucleic acid sequence is determined." [] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string created_by: jbmunro creation_date: 2018-05-17T12:00:00Z [Term] id: ECO:0001534 name: nucleic acid localization evidence def: "A type of localization evidence based on localization of a specific segment of DNA or RNA within tissue by the application of a complementary strand of nucleic acid to which a reporter molecule (i.e. either radio-, fluorescent- or antigen-labeled probe) is attached and quantified using either autoradiography, fluorescence microscopy, or immunohistochemistry." [] is_a: ECO:0001533 ! localization evidence property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string created_by: jbmunro creation_date: 2018-05-17T12:00:00Z [Term] id: ECO:0001546 name: acetylation assay evidence def: "A type of direct assay evidence where acetylated residues are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001547 name: cleavage assay evidence def: "A type of direct assay evidence where the cleavage of a protein into protein fragments by a protease is detected." [PMID:21121091, PMID:22154596] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001548 name: deacetylation assay evidence def: "A type of direct assay evidence where the removal of acetyl groups are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001549 name: defarnesylation assay evidence def: "A type of direct assay evidence where the removal of farnesyl groups from a protein is detected." [PMID:15556768, PMID:16126733, PMID:16507103, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001550 name: demethylation assay evidence def: "A type of direct assay evidence where the removal of methyl groups from a substrate (RNA/DNA or protein) is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001551 name: desumoylation assay evidence def: "A type of direct assay evidence where the removal of sumo groups from a protein is detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001552 name: deubiquitination assay evidence def: "A type of direct assay evidence where the removal of ubiquitin groups from a protein is detected." [PMID:19692941, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001553 name: farnesylation assay evidence def: "A type of direct assay evidence where farnesylated residues in proteins are detected." [PMID:9030603, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001554 name: methylation assay evidence def: "A type of direct assay evidence where methylated residues of a substarte (RNA/DNA or protein) are detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001555 name: palmitoylation assay evidence def: "A type of direct assay evidence where palmitoylated residues in a protein are detected." [PMID:10329400, PMID:17077383, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001556 name: phosphatase assay evidence def: "A type of direct assay evidence where the removal of phosphatase groups from a protein is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001557 name: polyADP-ribosylation assay evidence def: "A type of direct assay evidence where ADP-ribosylated residues in proteins are detected." [PMID:21870253, PMID:2820766, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001558 name: protein kinase assay evidence def: "A type of enzymatic activity assay evidence that measures transfer of a phosphate to a peptide or protein substrate by a protein kinase." [url:https\://www.ncbi.nlm.nih.gov/books/NBK91991/] is_a: ECO:0000005 ! enzymatic activity assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: jbmunro created_by: mchibucos creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001559 name: sumoylation assay evidence def: "A type of direct assay evidence where sumoylated residues on a protein are detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:000156 name: single-cell RNA-sequencing evidence used in automatic assertion def: "A type of single cell RNA-sequencing evidence that is used in automatic assertion." [] is_a: ECO:0001560 ! single-cell RNA-sequencing evidence is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion intersection_of: ECO:0001560 ! single-cell RNA-sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-01-02T12:00:00Z [Term] id: ECO:0001560 name: single-cell RNA-sequencing evidence def: "A type of RNA-sequencing evidence that uses a single cell as the source of the RNA." [] synonym: "scRNA-seq evidence" EXACT [] is_a: ECO:0000295 ! RNA-sequencing evidence property_value: IAO:0000234 "Bgee" xsd:string created_by: jbmunro creation_date: 2019-01-02T12:00:00Z [Term] id: ECO:0001561 name: translation assay evidence def: "A type of direct assay evidence where de novo protein synthesis is detected." [PMID:18230759, PMID:24901308, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001562 name: ubiquitination assay evidence def: "A type of direct assay evidence where ubiquitinated residues on a protein are detected." [PMID:19692941, SIB:PG] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001563 name: cell growth assay evidence def: "A type of direct assay evidence where biological cell development, i.e. an increase in cell mass and size are measured." [GO:0016049, PMID:11057898] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000112 "However, as shown in Fig. 5A, the recombinant mycobacterial cells became sensitive to the anti-TB drugs isoniazid and streptomycin, as evidenced by their inhibited growth in the presence of 25 mug/mL of isoniazid or 0.5 mug/mL of streptomycin in the medium." xsd:string {xref="PMID:20843371"} property_value: IAO:0000112 "Similar growth profiles of these strains were observed in liquid media with the corresponding inducers (data not shown). These indicate that the expression of vapC10 alone led to growth arrest of E. coli, and the simultaneous expression of vapB10 could neutralize this growth-inhibition effect, suggesting that vapC10 encodes a TA toxin and vapB10 encodes the cognate antitoxin." xsd:string {xref="PMID:24260461"} property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string created_by: mchibucos / jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001565 name: cell-based assay evidence def: "A type of direct assay evidence resulting from the study of cells." [] is_a: ECO:0000002 ! direct assay evidence created_by: jbmunro creation_date: 2017-11-30T14:00:00Z [Term] id: ECO:0001566 name: quantitative reverse transcription polymerase chain reaction evidence def: "A type of reverse transcription polymerase chain reaction evidence that combines reverse transcription polymerase chain reaction and real time polymerase chain reaction to quantitatively assay for the detection of RNA levels." [] synonym: "qRT-PCR evidence" EXACT [] synonym: "quantitative real-time RT-PCR evidence" EXACT [] synonym: "quantitative RT-PCR evidence" EXACT [] synonym: "real-time qRT-PCR evidence" EXACT [] synonym: "real-time quantitative reverse transcription PCR evidence" EXACT [] synonym: "real-time reverse transcription polymerase chain reaction evidence" EXACT [] synonym: "real-time RT-PCR evidence" EXACT [] synonym: "RRT-PCR evidence" EXACT [] synonym: "rRT-PCR evidence" EXACT [] synonym: "RT-qPCR evidence" EXACT [] is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence property_value: IAO:0000112 "The real-time RT-PCR validated that Zur repressed the first gene of each of the three operons, znuA, znuCB and ykgM-rpmJ2 (Additional file 5)." xsd:string {xref="PMID:19552825"} created_by: jbmunro creation_date: 2018-07-07T12:00:00Z [Term] id: ECO:0001567 name: quantitative reverse transcription polymerase chain reaction evidence used in manual assertion def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in manual assertion." [] is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence is_a: ECO:0001808 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence used in manual assertion intersection_of: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: jbmunro creation_date: 2018-07-07T12:00:00Z [Term] id: ECO:0001568 name: quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion. alt_id: ECO:000156 def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion." [] is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence is_a: ECO:0007475 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence used in automatic assertion intersection_of: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2018-07-07T12:00:00Z [Term] id: ECO:000157 name: colony diameter phenotype evidence used in manual assertion def: "A type of colony diameter phenotype evidence that is used in manual assertion." [] is_a: ECO:0001571 ! colony diameter phenotype evidence is_a: ECO:0007082 {is_inferred="true"} ! colony size phenotypic evidence used in manual assertion intersection_of: ECO:0001571 ! colony diameter phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: jbmunro creation_date: 2019-01-03T12:00:00Z [Term] id: ECO:0001570 name: single-cell RNA-sequencing evidence used in manual assertion def: "A type of single cell RNA-sequencing evidence that is used in manual assertion." [] is_a: ECO:0001560 ! single-cell RNA-sequencing evidence is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion intersection_of: ECO:0001560 ! single-cell RNA-sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: jbmunro creation_date: 2019-01-02T12:00:00Z [Term] id: ECO:0001571 name: colony diameter phenotype evidence def: "A type of colony size phenotypic evidence resulting from measurement of a microbial colony's diameter." [] is_a: ECO:0007081 ! colony size phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-01-03T12:00:00Z [Term] id: ECO:0001572 name: colony diameter phenotype evidence used in automatic assertion def: "A type of colony diameter phenotype evidence that is used in automatic assertion" [] is_a: ECO:0001571 ! colony diameter phenotype evidence is_a: ECO:0007627 {is_inferred="true"} ! colony size phenotypic evidence used in automatic assertion intersection_of: ECO:0001571 ! colony diameter phenotype evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-01-03T12:00:00Z [Term] id: ECO:0001574 name: membrane fusion assay evidence def: "A type of direct assay evidence in which a broad variety of physiological processes are measured when two separate membranes merge into a single contiguous membrane." [PMID:1836993] comment: Measurements can include but are not limited to: synaptic transmission, fertilization, and viral entry. is_a: ECO:0000002 ! direct assay evidence created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001575 name: membrane fusion assay evidence used in automatic assertion def: "A type of membrane fusion assay evidence that is used in automatic assertion." [] is_a: ECO:0001574 {is_inferred="true"} ! membrane fusion assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001574 ! membrane fusion assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001576 name: membrane fusion assay evidence used in manual assertion def: "A type of membrane fusion assay evidence that is used in manual assertion." [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0001574 {is_inferred="true"} ! membrane fusion assay evidence intersection_of: ECO:0001574 ! membrane fusion assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001577 name: spheroplast fusion assay evidence def: "A type of membrane fusion assay evidence based on the fusion of spheroplasts." [] comment: A spheroplast is a Gram-negative bacterium cell in which the cell wall has been almost completely removed. {xref="PMID:25870259"} is_a: ECO:0001574 ! membrane fusion assay evidence created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001578 name: spheroplast fusion assay evidence used in automatic assertion def: "A type of spheroplast fusion assay evidence used in automatic assertion." [] is_a: ECO:0001575 {is_inferred="true"} ! membrane fusion assay evidence used in automatic assertion is_a: ECO:0001577 {is_inferred="true"} ! spheroplast fusion assay evidence intersection_of: ECO:0001577 ! spheroplast fusion assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001579 name: spheroplast fusion assay evidence used in manual assertion def: "A type of spheroplast fusion assay evidence that is used in manual assertion." [] is_a: ECO:0001576 {is_inferred="true"} ! membrane fusion assay evidence used in manual assertion is_a: ECO:0001577 {is_inferred="true"} ! spheroplast fusion assay evidence intersection_of: ECO:0001577 ! spheroplast fusion assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: jbmunro creation_date: 2019-03-01T12:00:00Z [Term] id: ECO:0001580 name: liquid chromatography coupled with tandem mass spectrometry evidence def: "A type of mass spectrometry evidence where liquid chromatography is used to separate particles in solution, followed by fragmentation and measurement of the mass-to-charge ratio of the resulting particles to identify the amount and type of material entities present in a sample." [] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-03-26T12:00:00Z [Term] id: ECO:0001581 name: liquid chromatography coupled with tandem mass spectrometry evidence used in automatic assertion def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in an automatic assertion." [] is_a: ECO:0001580 {is_inferred="true"} ! liquid chromatography coupled with tandem mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-03-26T12:00:00Z [Term] id: ECO:0001582 name: liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in a manual assertion." [] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0001580 {is_inferred="true"} ! liquid chromatography coupled with tandem mass spectrometry evidence intersection_of: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-03-26T12:00:00Z [Term] id: ECO:0001583 name: small interfering RNA knockdown evidence def: "A type of anti-sense experiment evidence where gene expression is disrupted through the introduction of double-stranded RNA molecules, 20-25 base pairs in length, which operate within the RNA interference pathway." [] synonym: "short interfering RNA" EXACT [] synonym: "silencing RNA" EXACT [] synonym: "siRNA" EXACT [] is_a: ECO:0000018 ! anti-sense experiment evidence property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-03T12:00:00Z [Term] id: ECO:0001584 name: small interfering RNA knockdown evidence used in automatic assertion def: "A type of small interfering RNA knockdown evidence that is used in an automatic assertion." [] is_a: ECO:0001583 ! small interfering RNA knockdown evidence is_a: ECO:0007556 {is_inferred="true"} ! anti-sense experiment evidence used in automatic assertion intersection_of: ECO:0001583 ! small interfering RNA knockdown evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-03T12:00:00Z [Term] id: ECO:0001585 name: small interfering RNA knockdown evidence used in manual assertion def: "A type of small interfering RNA knockdown evidence that is used in a manual assertion." [] is_a: ECO:0001583 ! small interfering RNA knockdown evidence is_a: ECO:0006050 {is_inferred="true"} ! anti-sense experiment evidence used in manual assertion intersection_of: ECO:0001583 ! small interfering RNA knockdown evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-03T12:00:00Z [Term] id: ECO:0001586 name: ion trap mass spectrometry evidence def: "A type of mass spectrometry evidence where a combination of magnetic and/or electric fields are utilized to capture charged particles in tandem with mass spectrometry." [PMID:1561330] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001587 name: ion trap mass spectrometry evidence used in automatic assertion def: "A type of ion trap mass spectrometry evidence that is used in an automatic assertion." [] is_a: ECO:0001586 ! ion trap mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0001586 ! ion trap mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001588 name: ion trap mass spectrometry evidence used in manual assertion def: "A type of ion trap mass spectrometry evidence that is used in a manual assertion." [] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0001586 ! ion trap mass spectrometry evidence intersection_of: ECO:0001586 ! ion trap mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001589 name: atomic force microscopy evidence def: "A type of microscopy evidence where a mechanical probe is applied to a sample (i.e. a type of scanning probe microscopy) which can result in production of an image (with resolution in the order of fractions of a nanometer), force measurement, or sample manipulation." [] comment: Atomic force microscopy can be used for force measurement (force spectroscopy), imaging (rendering a three-dimensional shape (topography), and manipulation of the sample. synonym: "AFM" EXACT [] synonym: "scanning force microscopy" EXACT [] synonym: "SFM" EXACT [] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000112 "AFM revealed loop structures stabilized by multiple EspR dimer of dimers suggesting the presence of several distant EspR binding sites in the espACD upstream region." xsd:string {xref="PMID:22479184"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001590 name: atomic force microscopy evidence used in automatic assertion def: "A type of atomic force microscopy evidence that is used in an automatic assertion." [] is_a: ECO:0001589 ! atomic force microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion intersection_of: ECO:0001589 ! atomic force microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001591 name: atomic force microscopy evidence used in manual assertion def: "A type of atomic force microscopy evidence that is used in a manual assertion." [] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0001589 ! atomic force microscopy evidence intersection_of: ECO:0001589 ! atomic force microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001592 name: polysome profiling evidence def: "A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined." [PMID:30545912] comment: Once isolated, gel electrophoresis, immunoblot, and other protein profiling techniques can be applied to garner more information. is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001593 name: polysome profiling evidence used in automatic assertion def: "A type of polysome profiling evidence that is used in an automatic assertion." [] is_a: ECO:0001592 ! polysome profiling evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0001592 ! polysome profiling evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001594 name: polysome profiling evidence used in manual assertion def: "A type of polysome profiling evidence that is used in a manual assertion." [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001592 ! polysome profiling evidence intersection_of: ECO:0001592 ! polysome profiling evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: jbmunro creation_date: 2019-04-15T12:00:00Z [Term] id: ECO:0001598 name: multilocus sequence typing evidence def: "A type of nucleotide sequencing assay evidence in which DNA sequence variations within a specific set of genes (typically housekeeping genes) are used to characterizes strains by their unique allelic profiles." [PMID:9501229] comment: See PMID:24713082 for use example. synonym: "MLST" EXACT [] is_a: ECO:0000219 ! nucleotide sequencing assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001599 name: multilocus sequence typing evidence used in automatic assertion def: "A type of multilocus sequence typing evidence that is used in an automatic assertion." [] is_a: ECO:0001598 ! multilocus sequence typing evidence is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0001598 ! multilocus sequence typing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001600 name: multilocus sequence typing evidence used in manual assertion def: "A type of multilocus sequence typing evidence that is used in a manual assertion" [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001598 ! multilocus sequence typing evidence intersection_of: ECO:0001598 ! multilocus sequence typing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001601 name: protein-oligonucleotide microarray binding evidence def: "A type of protein-binding evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] synonym: "PBM evidence" EXACT [] is_a: ECO:0000024 ! protein binding evidence is_a: ECO:0000136 ! nucleic acid binding evidence created_by: swolfish creation_date: 2015-06-05T14:36:00Z [Term] id: ECO:0001603 name: cell staining evidence def: "A type of cell-based assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death." [GOC:MAH] synonym: "vital staining evidence" RELATED [GOC:MAH] is_a: ECO:0001565 ! cell-based assay evidence created_by: swolfish creation_date: 2015-06-08T11:51:37Z [Term] id: ECO:0001801 name: alkaline phosphatase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing." [ECO:SW, PMID:11823238] comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane. synonym: "SEAP reporter assay" RELATED [] synonym: "Secreted Embryonic Alkaline Phosphatase reporter assay" RELATED [] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T15:53:45Z [Term] id: ECO:0001802 name: beta-galactosidase reporter gene assay evidence alt_id: ECO:0000297 def: "A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium." [ECO:SW, PMID:20439410] comment: The assay is often performed using a plasmid borne construction on a lacZ strain. synonym: "beta-gal reporter gene assay" EXACT [] synonym: "beta-galactosidase assay evidence" EXACT [] synonym: "lacZ reporter gene assay" RELATED [] synonym: "LacZ transcript localization evidence" EXACT [] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000112 "For each of the three genes, there was a significant increase of beta-galactosidase activity in Deltazur compared to WT when they grew in TMH with the addition of zinc. Thus, Zur repressed the promoter activities of znuC, znuA and ykgM." xsd:string {xref="PMID:19552825"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T15:57:10Z [Term] id: ECO:0001803 name: chloramphenicol acetyltransferase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic." [ECO:SW, PMID:1630936] comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present. synonym: "CAT reporter gene assay" EXACT [PMID:1630936] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T16:06:42Z [Term] id: ECO:0001804 name: beta-glucuronidase reporter gene assay evidence def: "A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection." [ECO:SW] synonym: "GUS reporter gene assay" EXACT [] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T16:11:02Z [Term] id: ECO:0001805 name: luciferase reporter gene assay evidence def: "A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence." [ECO:SW, PMID:17623934] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T16:15:39Z [Term] id: ECO:0001806 name: chromatin immunoprecipitation- exonuclease evidence def: "A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data." [ECO:SW, PMID:25249628] synonym: "ChIP-exo" EXACT [PMID:25249628] is_a: ECO:0000226 ! chromatin immunoprecipitation evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-10-29T16:37:36Z [Term] id: ECO:0001807 name: electrophoretic mobility shift assay evidence used in manual assertion def: "A type of electrophoretic mobility shift that is used in a manual assertion." [ECO:SW] synonym: "electrophoretic mobility shift assay" BROAD [] synonym: "EMSA evidence" BROAD [] synonym: "gel retardation assay" RELATED [] is_a: ECO:0000096 ! electrophoretic mobility shift assay evidence is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion intersection_of: ECO:0000096 ! electrophoretic mobility shift assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: swolfish creation_date: 2015-11-03T12:13:17Z [Term] id: ECO:0001808 name: reverse transcription polymerase chain reaction evidence used in manual assertion def: "A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion." [ECO:SW] is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000109 ! reverse transcription polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: swolfish creation_date: 2015-11-03T12:22:46Z [Term] id: ECO:0001809 name: DNA affinity chromatography evidence def: "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." [ECO:SW, PMID:11694305, PMID:11725488] comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture. synonym: "DNA affinity purification" RELATED [] is_a: ECO:0000325 ! chromatography evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T13:48:49Z [Term] id: ECO:0001810 name: DNAse footprinting evidence def: "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." [ECO:SW, PMID:212715, PMID:22194258] comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites. synonym: "DNase protection" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000112 "Subsequent DNase I footprinting experiments (Fig. 5d) indicated that His-PhoP protected a single region located from 102 to 47 bp upstream of rovA. This footprint was considered the PhoP site." xsd:string {xref="PMID:21966533"} property_value: IAO:0000112 "The subsequent DNase I footprinting experiments (Figure 3a) showed that His-OmpR-P protected a single region within the ompR promoter. Therefore, OmpR stimulated its own gene at the transcriptional level, which was mediated through the binding of OmpR-P to its own promoter." xsd:string {xref="PMID:21345178"} property_value: IAO:0000112 "To precisely determine the PhoP-binding sites of target genes, DNase I footprinting assay was performed on 17 PhoP-dependent promoter DNA regions (both coding and noncoding strands) (Additional file 6). DNase I footprinting results confirmed the direct binding of His-PhoP to these promoter regions in vitro." xsd:string {xref="PMID:18366809"} property_value: IAO:0000112 "We therefore pursued DNase I footprinting assays on several of the Crp-associated sequences that gave an unusual downshift in our initial EMSA trials, in an effort to identify a consensus binding sequence (see Fig. S2 in the supplemental material). We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T13:55:15Z [Term] id: ECO:0001811 name: fluorescence anisotropy evidence def: "A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules." [ECO:SW, PMID:20232898] comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous. synonym: "FA" EXACT [] synonym: "Fluorescence polarization" RELATED [PMC:PMC3277431, PMID:20232898] synonym: "FP" RELATED [PMC:PMC3277431, PMID:20232898] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000112 "Since these FA analyses are conducted with 300 nM scOhrR, this suggests that oxidation leads to at least a 100-fold reduction in DNA-binding affinity." xsd:string {xref="PMID:19129220"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T14:01:36Z [Term] id: ECO:0001812 name: genomic systematic evolution of ligands by exponential amplification evidence def: "A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing." [ECO:SW, PMID:20541015, PMID:21720957] comment: The reported sequences should always be verified for presence in the genome sequence. synonym: "genomic SELEX" EXACT [PMID:20541015] is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T14:05:37Z [Term] id: ECO:0001813 name: heteronuclear single quantum coherence spectroscopy evidence def: "A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum." [ECO:SW, PMID:19856946] comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site. synonym: "HSQC" EXACT [PMID:19856946] is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T14:11:06Z [Term] id: ECO:0001814 name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence def: "A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA." [ECO:SW, PMID:6225070] synonym: "Methidiumpropyl-EDTA Fe(II) footprinting" RELATED [PMID:6225070] synonym: "MPE Fe(II) footprinting" RELATED [PMID:6225070] synonym: "MPE-EDTA Fe(II) footprinting" RELATED [PMID:6225070] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-03T14:24:04Z [Term] id: ECO:0001815 name: copper-phenanthroline footprinting evidence def: "A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts." [ECO:SW, PMID:11691942] synonym: "1,10-Phenanthroline-copper footprinting" EXACT [PMID:1384472] synonym: "OP-Cu Complex" RELATED [PMID:1384472] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T08:37:51Z [Term] id: ECO:0001816 name: green fluorescent protein reporter gene assay evidence alt_id: ECO:0000296 def: "A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light." [ECO:SW, PMID:11989662] synonym: "GFP promoter fusion" RELATED [] synonym: "GFP reporter gene assay" EXACT [PMID:11989662] synonym: "green fluorescent protein promoter fusion" RELATED [] synonym: "green fluorescent protein transcript localization evidence" EXACT [] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T08:58:42Z [Term] id: ECO:0001817 name: glutathione S-transferase pull-down assay evidence def: "A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." [ECO:SW, PMID:26096507] comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing. synonym: "GST pull-down assay" EXACT [PMID:26096507] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T09:05:56Z [Term] id: ECO:0001818 name: hydroxyl-radical footprinting evidence def: "A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:18546600, PMID:19378159, PMID:3090544] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T09:20:26Z [Term] id: ECO:0001819 name: primer extension assay evidence def: "A type of transcript expression evidence used to determine expression levels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site)." [ECO:SW, PMID:23378648] comment: The most commonly used radiolabel is 32P. is_a: ECO:0000009 ! transcript expression evidence property_value: IAO:0000112 "The primer extension assay (Fig. 6a) defined the transcription start sites the three sRNA genes qrr2 - 4, and this assay also indicated that the promoter activity of all the thee qrr genes was under the positive control of OpaR." xsd:string {xref="PMID:22506036"} property_value: IAO:0000112 "The primer extension assay detected two transcriptional start sites located at 343 and 78 bp upstream of rovA (Fig. 2); therefore, two promoters (named P2 and P1, respectively) were transcribed for rovA." xsd:string {xref="PMID:21966533"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T09:33:18Z [Term] id: ECO:0001820 name: rapid amplification of cDNA ends polymerase chain reaction evidence def: "A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken." [ECO:SW, PMID:17498297, PMID:7685466] comment: 5' and 3' RACE-PCR utilize different protocols to amplify an unknown end using the known sequence of the center of the transcript. For cDNA synthesis, 5' RACE-PCR uses an anti-sense oligonucleotide primer (gene specific primer (GSP)) that recognizes a known sequence in the middle of the gene of interest and the reverse transcriptase to add base pairs to the 3' end of the primer. Next, terminal deoxynucleotidyl transferase (TdT) is used to add a string of identical nucleotides to the 3' end of the cDNA. A PCR reaction is then carried out, using a second anti-sense gene specific primer (GSP2) that binds to the known sequence, and a sense (forward) universal primer (UP) that binds the homopolymeric tail added to the 3' ends of the cDNAs to amplify a cDNA product from the 5' end. In contrast, 3' RACE-PCR utilizes the naturally occurring 3' polyA tail of the transcript and uses an Oligo-dT-adaptor primer (a primer with a short sequence of deoxy-thymine nucleotides) that complements the polyA tail and adds a special adaptor sequence to the 5' end of each cDNA. PCR is then used to amplify 3' cDNA from a known region using a sense GSP, and an anti-sense primer complementary to the adaptor sequence. comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression. synonym: "3’ RACE-PCR" EXACT [] synonym: "5’ RACE-PCR" EXACT [] synonym: "RACE PCR" EXACT [PMI:17498297] is_a: ECO:0000219 ! nucleotide sequencing assay evidence property_value: IAO:0000112 "5' RACE analysis was employed to localize the espR promoter using RNA extracted from Mtb H37Rv grown to mid-log phase. The espR transcript starts with a poly-G (7) sequence 144 bp upstream of the translational start codon." xsd:string {xref="PMID:22479184"} property_value: IAO:0000112 "The 5'-end of the espA mRNA was located 66 bp upstream of the translation start codon using 5' RACE (Fig. S3)." xsd:string {xref="PMID:22479184"} property_value: IAO:0000112 "Using 5' rapid amplification of cDNA ends (RACE), we mapped the transcriptional start sites of rpoQ and VF_A1016 (Fig. 4A). Neither start site was detected in the DeltarpoQ mutant, supporting the idea that transcription of rpoQ and VF_A1016 requires RpoQ (data not shown)." xsd:string {xref="PMID:22233679"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T09:48:38Z [Term] id: ECO:0001821 name: RNA sequencing assay evidence comment: Use ECO:0000295 (RNA-seq evidence) in place of this term. property_value: IAO:0000234 "CollecTF" xsd:string is_obsolete: true created_by: swolfish creation_date: 2015-11-04T10:15:57Z [Term] id: ECO:0001822 name: survival rate analysis evidence def: "A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter." [ECO:SW] is_a: ECO:0001091 ! knockout phenotypic evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T10:48:59Z [Term] id: ECO:0001823 name: x-ray crystallography evidence def: "A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules." [ECO:SW, PMID:23135450, PMID:24648090] comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components. is_a: ECO:0001034 ! crystallography evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-04T10:56:22Z [Term] id: ECO:0001824 name: DNA adenine methyltransferase identification evidence def: "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092] comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII. synonym: "DamID" EXACT [PMID:19588092] is_a: ECO:0000023 ! affinity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-05T15:56:13Z [Term] id: ECO:0001825 name: isothermal titration calorimetry evidence def: "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." [ECO:SW, PMID:10527727] comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)). synonym: "ITC" EXACT [PMID:10527727] is_a: ECO:0000023 ! affinity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-05T16:06:20Z [Term] id: ECO:0001826 name: ultraviolet light footprinting evidence def: "A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts." [ECO:SW, PMID:2842760, PMID:7602584] synonym: "ultraviolet footprinting" RELATED [] synonym: "UV footprinting" RELATED [] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-05T16:13:36Z [Term] id: ECO:0001827 name: methylation interference footprinting evidence def: "A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:1583685] synonym: "methylation interference footprinting evidence" EXACT [] is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-05T16:16:27Z [Term] id: ECO:0001828 name: inference of sequence features from visual inspection used in manual assertion def: "A type of inference of sequence features from visual inspection that is used in a manual assertion." [ECO:RCJ] synonym: "visual sequence inspection evidence" BROAD [] is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion is_a: ECO:0007850 {is_inferred="true"} ! inference of sequence features from visual inspection intersection_of: ECO:0007850 ! inference of sequence features from visual inspection intersection_of: used_in ECO:0000218 ! manual assertion created_by: swolfish creation_date: 2015-11-10T14:40:16Z [Term] id: ECO:0001829 name: ferric uptake regulator titration assay evidence def: "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138] synonym: "Fluorescence polarization" RELATED [PMID:15689115] synonym: "FURTA" EXACT [PMID:10713425] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: swolfish creation_date: 2015-11-10T14:55:36Z [Term] id: ECO:0001830 name: host colonization assay evidence def: "A type of experimental evidence in which the colonization or adhesion capacity is measured." [] comment: Has to do with the ability of a symbiont (any organism living in close association with a host, including both beneficial and parasitic relationships) to bind to a host. synonym: "adhesion assay evidence" EXACT [] is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001831 name: host colonization assay evidence used in automatic assertion def: "A type of host colonization assay evidence that is used in an automatic assertion." [] is_a: ECO:0001830 ! host colonization assay evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0001830 ! host colonization assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001832 name: host colonization assay evidence used in manual assertion def: "A type of host colonization assay evidence that is used in a manual assertion." [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001830 ! host colonization assay evidence intersection_of: ECO:0001830 ! host colonization assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001833 name: infection assay evidence def: "A type of host colonization assay evidence in which the infection capacity is measured by assessing changes in the host." [] comment: Has to do with the outcome of a bacterial or viral / host interaction, be it a measure of cell death, cytotoxicity, invasion, etc. is_a: ECO:0001830 ! host colonization assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001834 name: infection assay evidence used in automatic assertion def: "A type of infection assay evidence that is used in an automatic assertion." [] is_a: ECO:0001831 {is_inferred="true"} ! host colonization assay evidence used in automatic assertion is_a: ECO:0001833 ! infection assay evidence intersection_of: ECO:0001833 ! infection assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001835 name: infection assay evidence used in manual assertion def: "A type of infection assay evidence that is used in a manual assertion." [] is_a: ECO:0001832 {is_inferred="true"} ! host colonization assay evidence used in manual assertion is_a: ECO:0001833 ! infection assay evidence intersection_of: ECO:0001833 ! infection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: jbmunro creation_date: 2019-05-06T12:00:00Z [Term] id: ECO:0001836 name: in situ hybridization evidence def: "A type of expression pattern evidence in which single-stranded DNA or RNA (probe) are annealed to complementary DNA or RNA in a portion or section of tissue." [] is_a: ECO:0000008 ! expression pattern evidence property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001837 name: in situ hybridization evidence used in automatic assertion def: "A type of in situ hybridization evidence that is used in an automatic assertion." [] is_a: ECO:0001836 ! in situ hybridization evidence is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion intersection_of: ECO:0001836 ! in situ hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001838 name: in situ hybridization evidence used in manual assertion def: "A type of in situ hybridization evidence that is used in a manual assertion." [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001836 ! in situ hybridization evidence intersection_of: ECO:0001836 ! in situ hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001839 name: colorimetric in situ hybridization evidence def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of colorimetric probes to detect complementary sequences of nucleic acids." [PMID:23497040] is_a: ECO:0001836 ! in situ hybridization evidence property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001840 name: colorimetric in situ hybridization evidence used in automatic assertion def: "A type of colorimetric in situ hybridization evidence that is used in an automatic assertion." [] is_a: ECO:0001837 {is_inferred="true"} ! in situ hybridization evidence used in automatic assertion is_a: ECO:0001839 ! colorimetric in situ hybridization evidence intersection_of: ECO:0001839 ! colorimetric in situ hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001841 name: colorimetric in situ hybridization evidence used in manual assertion def: "A type of colorimetric in situ hybridization evidence that is used in a manual assertion." [] is_a: ECO:0001838 {is_inferred="true"} ! in situ hybridization evidence used in manual assertion is_a: ECO:0001839 ! colorimetric in situ hybridization evidence intersection_of: ECO:0001839 ! colorimetric in situ hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: jbmunro creation_date: 2019-09-26T12:00:00Z [Term] id: ECO:0001842 name: random mutagenesis of specific target DNA evidence def: "A type of random mutagenesis phenotypic evidence resulting from the random mutation of a specifically targeted region of DNA." [] is_a: ECO:0005529 ! random mutagenesis phenotypic evidence property_value: IAO:0000234 "ECO" xsd:string created_by: jbmunro creation_date: 2019-12-09T12:00:00Z [Term] id: ECO:0001843 name: random mutagenesis of specific target DNA evidence used in automatic assertion def: "A type of random mutagenesis of specific target DNA evidence that is used in an automatic assertion." [] is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence is_a: ECO:0007478 {is_inferred="true"} ! random mutagenesis evidence used in automatic assertion intersection_of: ECO:0001842 ! random mutagenesis of specific target DNA evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "ECO" xsd:string created_by: jbmunro creation_date: 2019-12-09T12:00:00Z [Term] id: ECO:0001844 name: random mutagenesis of specific target DNA evidence used in manual assertion def: "A type of random mutagenesis of specific target DNA evidence that is used in a manual assertion." [] is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence is_a: ECO:0005530 {is_inferred="true"} ! random mutagenesis evidence used in manual assertion intersection_of: ECO:0001842 ! random mutagenesis of specific target DNA evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 "ECO" xsd:string created_by: jbmunro creation_date: 2019-12-09T12:00:00Z [Term] id: ECO:0001845 name: cell population optical density evidence def: "A type of cell-based assay evidence in which optical density is used to measure some aspect of a population of cells. Examples of aspects that can affect optical density measurements include, but are not limited to, cell number and cell shape." [] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 "CACAO" xsd:string created_by: jbmunro creation_date: 2020-01-28T12:00:00Z [Term] id: ECO:0001846 name: cell population optical density evidence used in automatic assertion def: "A type of cell population optical density evidence that is used in an automatic assertion." [] is_a: ECO:0001845 ! cell population optical density evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001845 ! cell population optical density evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "CACAO" xsd:string created_by: jbmunro creation_date: 2020-01-28T12:00:00Z [Term] id: ECO:0001847 name: cell population optical density evidence used in manual assertion def: "A type of cell population optical density evidence that is used in a manual assertion." [] is_a: ECO:0001845 ! cell population optical density evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001845 ! cell population optical density evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "CACAO" xsd:string created_by: jbmunro creation_date: 2020-01-28T12:00:00Z [Term] id: ECO:0005004 name: cell viability assay evidence def: "A type of cell-based assay evidence resulting from analyzing the ability of cells to survive or live successfully." [NBK:144065] is_a: ECO:0001565 ! cell-based assay evidence created_by: snadendla creation_date: 2015-02-13T12:08:01Z [Term] id: ECO:0005007 name: cell proliferation assay evidence def: "A type of cell-based assay evidence resulting from the measurement of the number of cells, which is a reflection of the balance between cell division and cell loss through cell differentiation or cell death." [ECO:RCT] {comment="PMID:11057898", comment="GO:0008283"} is_a: ECO:0001565 ! cell-based assay evidence created_by: snadendla creation_date: 2015-02-11T16:27:13Z [Term] id: ECO:0005008 name: DNA synthesis cell proliferation assay evidence def: "A type of cell proliferation assay evidence resulting from the measure of DNA synthesis in the presece of a label." [PMC:PMC3908118] is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2015-02-11T16:30:10Z [Term] id: ECO:0005011 name: ATP bioluminescence assay evidence def: "A type of cell viability assay evidence resulting from the analysis of ATP concentration (by measuring light intensity) when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions and molecular oxygen." [PMID:15344559] is_a: ECO:0005004 ! cell viability assay evidence created_by: snadendla creation_date: 2015-02-11T16:47:30Z [Term] id: ECO:0005012 name: cytotoxicity assay evidence def: "A type of cell-based assay evidence resulting from the ability of an agent to induce cellular necrosis or apoptosis and the subsequent analysis of cell death mechanism." [] is_a: ECO:0001565 ! cell-based assay evidence created_by: snadendla creation_date: 2015-02-12T11:39:35Z [Term] id: ECO:0005014 name: in vitro cell based assay evidence comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. is_obsolete: true created_by: snadendla creation_date: 2015-02-12T13:26:55Z [Term] id: ECO:0005019 name: staining evidence def: "A type of direct assay evidence resulting from the use of stains or dyes to aid in analysis of a microscopic image." [ECO:RCT] is_a: ECO:0000002 ! direct assay evidence created_by: snadendla creation_date: 2015-02-19T11:48:57Z [Term] id: ECO:0005021 name: chemotaxis assay evidence def: "A type of cell-based assay evidence resulting from the evaluation of chemotactic ability (i.e. the movement, or lack of movement, in response to a chemical stimulus) of prokaryotic or eukaryotic cells." [PMC:PMC3667641] is_a: ECO:0001565 ! cell-based assay evidence created_by: snadendla creation_date: 2015-02-19T13:10:20Z [Term] id: ECO:0005027 name: genetic transformation evidence def: "A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA." [ECO:RCT] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-03-16T15:33:50Z [Term] id: ECO:0005031 name: structure determination evidence def: "A type of experimental evidence resulting from the visualization and examination of chemical and/or molecular structures." [ECO:RCT] is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} created_by: snadendla creation_date: 2015-03-20T12:16:17Z [Term] id: ECO:0005033 name: electron microscopy evidence def: "A type of microscopy evidence and structure determination evidence resulting from an electron beam utilized to create an image." [ECO:RCT] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000112 "Electron micrographs showed that all the cells are rod-shaped and those of the parent strain Ye9 and the envZ mutant strain EZ10 are flagellated, but the ompR mutant cells lack flagella." xsd:string {xref="PMID:20830609"} created_by: snadendla creation_date: 2015-03-20T12:21:48Z [Term] id: ECO:0005034 name: apoptotic assay evidence def: "A type of cell-based assay evidence resulting from the occurrence, visualization, and/or analysis of apoptosis." [ECO:RCT] is_a: ECO:0001565 ! cell-based assay evidence created_by: snadendla creation_date: 2015-04-07T21:58:24Z [Term] id: ECO:0005500 name: in vivo polyADP-ribosylation assay evidence def: "A type of polyADP-ribosylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:2820766, SIB:PG] is_a: ECO:0001557 ! polyADP-ribosylation assay evidence created_by: snadendla creation_date: 2015-06-15T15:35:05Z [Term] id: ECO:0005501 name: in vivo polyADP-ribosylation assay evidence used in manual assertion def: "A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence intersection_of: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2015-06-15T15:37:23Z [Term] id: ECO:0005502 name: ex vivo assay evidence def: "A type of direct assay evidence derived by studying an organ, tissues, or cells taken from an organism and studied in an external environment with the minimum alteration of natural conditions." [ECO:SN, PMID:16305312, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] is_a: ECO:0000002 ! direct assay evidence created_by: snadendla creation_date: 2015-06-26T11:46:15Z [Term] id: ECO:0005503 name: two-dimensional polyacrylamide gel electrophoresis evidence def: "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." [ECO:SW, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html] comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes. synonym: "2D PAGE" EXACT [] synonym: "2D-PAGE" EXACT [] is_a: ECO:0000337 ! gel electrophoresis evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-01T16:23:20Z [Term] id: ECO:0005504 name: spectrometry evidence def: "A type of experimental evidence resulting from the use of a spectrometer." [ECO:RCT] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2015-08-14T15:48:08Z [Term] id: ECO:0005505 name: regular expression motif search evidence def: "A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression." [ECO:SW, PMID:17337630] comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID:20708667). is_a: ECO:0000028 ! motif similarity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-09T13:51:52Z [Term] id: ECO:0005506 name: missense mutation phenotypic evidence def: "A type of point mutation phenotypic evidence resulting from a change in a base (or bases) causing the substitution of one amino acid for another." [SO:0001583] synonym: "missense mutation evidence" EXACT [] is_a: ECO:0001113 ! point mutation phenotypic evidence created_by: snadendla creation_date: 2015-09-18T16:52:34Z [Term] id: ECO:0005507 name: nonsense mutation phenotypic evidence def: "A type of point mutation phenotypic evidence resulting in an incomplete and usually nonfunctional protein product because of a premature stop codon." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] synonym: "nonsense mutation evidence" EXACT [] is_a: ECO:0001113 ! point mutation phenotypic evidence created_by: snadendla creation_date: 2015-09-18T16:55:32Z [Term] id: ECO:0005508 name: silent mutation evidence def: "A type of point mutation phenotypic evidence resulting from the change of a DNA nucleotide that does not cause any changes in the amino acid sequence." [url:http\://www.sciencemag.org/news/2006/12/sound-silent-mutation] is_a: ECO:0001113 ! point mutation phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:01:12Z [Term] id: ECO:0005509 name: insertion mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from a mutation in which there is an insertion of one or more contiguous nucleotides." [GO:0070705] synonym: "insertion mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-09-18T17:02:03Z [Term] id: ECO:0005511 name: duplication mutation evidence def: "A type of experimental phenotypic evidence resulting from a mutation in which inserted nucleotide(s) have a sequence identical to, or derived from, adjacent nucleotides." [SO:1000035] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: snadendla creation_date: 2015-09-18T17:03:25Z [Term] id: ECO:0005512 name: frameshift mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from any mutation that causes an insertion or deletion of nucleotides in a DNA sequence that is not divisible by three and therefore changes the codon reading frame and the translated amino acids." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] synonym: "frameshift mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-09-18T17:05:49Z [Term] id: ECO:0005513 name: repeat expansion mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from a mutation that increases the number of times that a DNA sequence is repeated." [PMID:9397685, url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] synonym: "repeat expansion mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-09-18T17:06:34Z [Term] id: ECO:0005514 name: splice site mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from a mutation in which there is a change in nucleotides at a splice site." [ECO:RCT] synonym: "splice site mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-09-18T17:07:29Z [Term] id: ECO:0005515 name: translocation mutation phenotypic evidence def: "A type of mutant phenotype evidence resulting from a mutation in which a region of a chromosome is transferred to a nonhomologous chromosome." [ECO:RCT] synonym: "translocation mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence created_by: snadendla creation_date: 2015-09-18T17:08:01Z [Term] id: ECO:0005516 name: molecule detection assay evidence def: "A type of experimental evidence resulting from the detection and analysis of molecular markers." [ECO:RCT] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2015-09-24T15:54:21Z [Term] id: ECO:0005517 name: protein detection assay evidence def: "A type of molecule detection assay evidence resulting from the detection and quantification of a protein, or multiple proteins." [ECO:RCT] is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T14:27:40Z [Term] id: ECO:0005518 name: RNA detection assay evidence def: "A type of molecule detection assay evidence resulting from the detection of specific RNAs." [ECO:RCT] is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T14:43:53Z [Term] id: ECO:0005519 name: DNA detection assay evidence def: "A type of molecule detection assay evidence resulting from the detection and visualization of a sequence of DNA." [ECO:RCT] is_a: ECO:0005516 ! molecule detection assay evidence created_by: snadendla creation_date: 2015-09-25T19:33:25Z [Term] id: ECO:0005520 name: interferometric reflectance imaging sensor evidence def: "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." [ECO:SW, PMID:21587155, PMID:24271115] comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively. synonym: "IRIS" EXACT [] synonym: "Spectral Reflectance Imaging Biosensor" EXACT [] synonym: "SRIB" EXACT [] is_a: ECO:0005516 ! molecule detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-28T16:01:01Z [Term] id: ECO:0005521 name: S1 nuclease protection assay evidence def: "A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA." [ECO:SW, PMID:21390683] is_a: ECO:0001106 ! nuclease protection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-28T17:16:56Z [Term] id: ECO:0005522 name: DNA dot blot assay evidence def: "A type of DNA detection assay evidence resulting from evaluation of the relative abundance of target sequences hybridizing to DNA samples that are immobilized on a nitrocellulose or nylon membrane." [PMID:18265189] is_a: ECO:0005519 ! DNA detection assay evidence created_by: snadendla creation_date: 2015-09-30T10:03:03Z [Term] id: ECO:0005523 name: DNA dot blot assay evidence used in manual assertion def: "A type of DNA dot blot assay evidence that is used in a manual assertion." [ECO:SN, ECO:SW] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005522 ! DNA dot blot assay evidence intersection_of: ECO:0005522 ! DNA dot blot assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2015-09-30T10:04:00Z [Term] id: ECO:0005526 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence def: "A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology." [ECO:SW, PMID:21964792, PMID:25354905] comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available. synonym: "MALDI-TOF Mass Spectrometry" EXACT [] synonym: "MALDI-TOF-MS" EXACT [] is_a: ECO:0005517 ! protein detection assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-30T10:51:56Z [Term] id: ECO:0005527 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion." [ECO:SN, ECO:SW] synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] synonym: "MALDI-TOF-MS evidence" BROAD [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2015-09-30T10:58:51Z [Term] id: ECO:0005528 name: site-directed mutagenesis phenotypic evidence def: "A type of mutant phenotype phenotype evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence." [ECO:SW, PMID:21204030, PMID:24011050, PMID:3541892] comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site. synonym: "site directed mutagenesis" EXACT [] synonym: "site-directed mutagenesis evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000112 "If the AGAA sequence is mutated CTCC (Pxyn2aM, see Table 1), no DNA-protein complex is formed under either repressing or inducing conditions (Figure 1). This result indicates that this part of the xyn2 promoter is essential for binding a transcription factor under repressing conditions." xsd:string {xref="PMID:21087492"} property_value: IAO:0000112 "To identify key residues required for specific GlnR binding we mutated the highly conserved AC-n9-AC and AT-n9-AC DNA binding motifs. Figure 4 shows that the highly conserved adenosine residues in the motif are critical as GlnR binding is abolished when these residues are mutated." xsd:string {xref="PMID:23642041"} property_value: IAO:0000112 "We also tested a hyaS promoter in which one (highest score) of the three putative AdpA-binding sites was mutated (at position -134 to -129, see Additional file 3: Figure S1a): the affinity of AdpA for this promoter region was reduced and one protein-DNA complex disappeared (Additional file 3: Figure S1b). These results suggest that one dimer of AdpA binds the adjacent sites -129 and -123 of S. lividans hyaS promoter and another dimer binds the -100 site resulting in the formation of the two DNA-AdpA complexes depicted in Figure 2." xsd:string {xref="PMID:24694298"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-30T11:34:41Z [Term] id: ECO:0005529 name: random mutagenesis phenotypic evidence def: "A type of mutant phenotype evidence where a mutagenic product or process is used to alter the nucleotide sequence and thus induce genetic mutation. Examples include, but are not limited to mutagenic compounds, irradiation, PCR w/ degenerate primers, etc." [ECO:SW, PMID:15153637, PMID:18265275] comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination. synonym: "random mutagenesis evidence" NARROW [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-30T11:55:03Z [Term] id: ECO:0005530 name: random mutagenesis evidence used in manual assertion def: "A type of random mutagenesis phenotypic evidence that is used in a manual assertion." [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005529 ! random mutagenesis phenotypic evidence intersection_of: ECO:0005529 ! random mutagenesis phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: snadendla creation_date: 2015-09-30T11:57:00Z [Term] id: ECO:0005531 name: motif discovery evidence def: "A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs." [ECO:SW, PMID:10812473, PMID:16845028, PMID:8211139] comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF, from peaks in ChIP-seq experiments with the TF, or from the comparative genomics analysis of the promoter regions of orthologs. {xref="PMID:19892760", xref="PMID:23032607"} is_a: ECO:0005555 ! multiple sequence alignment evidence property_value: IAO:0000112 "A sequence alignment of the phlA gene promoter region from strain 2P24 with other well-studied 2,4-DAPG producing Pseudomonas strains revealed a very well conserved palindromic sequence GAAACN5GTTTC (Fig. S1)." xsd:string {xref="PMID:23209661"} property_value: IAO:0000112 "In order to identify a potential regulatory motif for KstR2, we used the promoter regions of the kstR2 orthologues as a training set for the motif identification program MEME (Bailey & Elkan, 1994). This identified a potential regulatory sequence that contains a 14 bp inverted palindromic motif AnCAAGnnCTTGnT (Fig. 2)." xsd:string {xref="PMID:20167624"} property_value: IAO:0000112 "We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-30T15:04:45Z [Term] id: ECO:0005532 name: consensus search evidence def: "A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match." [ECO:SW, PMID:15130839] comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID:17085555. is_a: ECO:0000028 ! motif similarity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-09T14:12:54Z [Term] id: ECO:0005533 name: phylogenetic footprinting evidence def: "A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'." [ECO:SW, PMID:16477324, PMID:3199442] comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID:11997340). is_a: ECO:0005531 ! motif discovery evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-09-30T16:23:02Z [Term] id: ECO:0005534 name: comparative genomics motif search evidence def: "A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes." [ECO:SW, PMID:10854408] comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID:22305460. Orthology is a typical comparative criterion used. is_a: ECO:0000028 ! motif similarity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-09T14:30:38Z [Term] id: ECO:0005535 name: machine learning prediction of motif instance evidence def: "A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences." [ECO:SW, PMID:17117497, PMID:2014171] comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID:15703297). is_a: ECO:0000202 ! match to sequence model evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-01T11:58:36Z [Term] id: ECO:0005536 name: position-specific scoring matrix motif search evidence def: "A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis." [ECO:SW, PMID:10812473] comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID:15604457). synonym: "PSSM" NARROW [] synonym: "PSSM motif search" EXACT [] is_a: ECO:0000028 ! motif similarity evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-09T15:04:35Z [Term] id: ECO:0005537 name: xylE reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene." [ECO:SW, PMID:2592344] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2015-10-28T15:31:28Z [Term] id: ECO:0005538 name: computationally derived logical inference is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:15:50Z [Term] id: ECO:0005539 name: computationally derived logical inference used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:17:47Z [Term] id: ECO:0005540 name: computationally derived logical inference from automatic assertion used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:24:42Z [Term] id: ECO:0005541 name: computationally derived logical inference from manual assertion used in automatic assertion is_obsolete: true created_by: snadendla creation_date: 2015-11-17T15:32:03Z [Term] id: ECO:0005542 name: biological system reconstruction evidence by experimental evidence from single species used in manual assertion def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion." [ECO:SN, PMID:19450514] comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. is_a: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species is_a: ECO:0007747 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in manual assertion intersection_of: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2015-12-16T15:32:41Z [Term] id: ECO:0005543 name: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion." [ECO:SN, PMID:22232657] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. is_a: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species is_a: ECO:0007747 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in manual assertion intersection_of: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2015-12-16T15:45:01Z [Term] id: ECO:0005544 name: biological system reconstruction evidence based on orthology evidence used in manual assertion def: "A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion." [ECO:SN] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion intersection_of: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISO" xsd:string created_by: snadendla creation_date: 2015-12-18T16:20:49Z [Term] id: ECO:0005545 name: biological system reconstruction evidence based on orthology evidence def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence created_by: snadendla creation_date: 2015-12-16T15:57:37Z [Term] id: ECO:0005546 name: biological system reconstruction evidence based on paralogy evidence used in manual assertion def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion intersection_of: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISS" xsd:string created_by: snadendla creation_date: 2015-12-16T16:05:15Z [Term] id: ECO:0005547 name: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion." [ECO:SN, PMID:17876790] comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. is_a: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge is_a: ECO:0007746 {is_inferred="true"} ! biological system reconstruction evidence used in manual assertion intersection_of: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "NAS" xsd:string created_by: snadendla creation_date: 2015-12-16T16:07:42Z [Term] id: ECO:0005548 name: biological system reconstruction evidence based on inference from background scientific knowledge def: "A type of biological system reconstruction based solely on background scientific knowledge of that biological system." [ECO:SN, PMID:17876790] comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T15:32:15Z [Term] id: ECO:0005549 name: biological system reconstruction evidence based on homology evidence def: "A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex." [ECO:SN, PMID:15660128] comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T15:49:46Z [Term] id: ECO:0005550 name: biological system reconstruction evidence based on paralogy evidence def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species." [ECO:SN, PMID:15660128] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence created_by: snadendla creation_date: 2015-12-18T16:24:08Z [Term] id: ECO:0005551 name: biological system reconstruction evidence by experimental evidence def: "A type of biological system reconstruction evidence that uses experimental evidence as support." [ECO:SN, PMID:19450514, PMID:22232657] is_a: ECO:0000088 ! biological system reconstruction evidence created_by: snadendla creation_date: 2015-12-18T16:34:13Z [Term] id: ECO:0005552 name: biological system reconstruction evidence by experimental evidence from mixed species def: "A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment." [ECO:SN, PMID:22232657] comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence created_by: snadendla creation_date: 2015-12-18T16:35:13Z [Term] id: ECO:0005553 name: biological system reconstruction evidence by experimental evidence from single species def: "A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species." [ECO:SN] comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence created_by: snadendla creation_date: 2015-12-18T16:36:44Z [Term] id: ECO:0005554 name: pairwise sequence alignment evidence def: "A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid)." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/psa/] is_a: ECO:0000200 ! sequence alignment evidence created_by: snadendla creation_date: 2016-02-08T14:30:46Z [Term] id: ECO:0005555 name: multiple sequence alignment evidence def: "A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/msa/] is_a: ECO:0000200 ! sequence alignment evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2016-02-08T14:31:24Z [Term] id: ECO:0005556 name: multiple sequence alignment evidence used in manual assertion def: "A type of multiple sequence alignment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0005555 ! multiple sequence alignment evidence intersection_of: ECO:0005555 ! multiple sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: snadendla creation_date: 2016-02-08T14:59:18Z [Term] id: ECO:0005557 name: multiple sequence alignment evidence used in automatic assertion def: "A type of multiple sequence alignment evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0005555 ! multiple sequence alignment evidence intersection_of: ECO:0005555 ! multiple sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-02-08T15:00:50Z [Term] id: ECO:0005558 name: motif discovery evidence used in manual assertion def: "A type of motif discovery evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005531 ! motif discovery evidence is_a: ECO:0005556 {is_inferred="true"} ! multiple sequence alignment evidence used in manual assertion intersection_of: ECO:0005531 ! motif discovery evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: snadendla creation_date: 2016-02-08T15:07:47Z [Term] id: ECO:0005559 name: motif discovery evidence used in automatic assertion def: "A type of motif discovery evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005531 ! motif discovery evidence is_a: ECO:0005557 {is_inferred="true"} ! multiple sequence alignment evidence used in automatic assertion intersection_of: ECO:0005531 ! motif discovery evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-02-08T15:08:19Z [Term] id: ECO:0005560 name: pairwise sequence alignment evidence used in manual assertion def: "A type of pairwise sequence alignment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: snadendla creation_date: 2016-02-08T15:17:05Z [Term] id: ECO:0005561 name: pairwise sequence alignment evidence used in automatic assertion def: "A type of pairwise sequence alignment evidence that is used in an automatic evidence." [ECO:SN] is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: ECO:0005554 ! pairwise sequence alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-02-08T15:17:52Z [Term] id: ECO:0005562 name: in-gel protein kinase assay evidence def: "A type of protein kinase assay evidence resulting from the evaluation of the phosphorylation activity of kinase on a gel-incorporated kinase substrate." [PMID:12372853] is_a: ECO:0001558 ! protein kinase assay evidence created_by: snadendla creation_date: 2016-02-09T12:05:29Z [Term] id: ECO:0005563 name: macroscopic current trace evidence def: "A type of voltage clamp recording evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording." [ECO:SN, PMID:17938230, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf] is_a: ECO:0005576 ! voltage clamp recording evidence created_by: snadendla creation_date: 2016-02-09T12:40:03Z [Term] id: ECO:0005564 name: current density evidence def: "A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane." [ECO:SN, url:http\://circres.ahajournals.org/content/102/11/1298.full, url:http\://www.bem.fi/book/04/04.htm] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:43:07Z [Term] id: ECO:0005565 name: single channel conductance evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:45:42Z [Term] id: ECO:0005566 name: sustained current evidence def: "A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels." [ECO:SN, url:http\://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:46:54Z [Term] id: ECO:0005567 name: steady state activation curve evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:48:17Z [Term] id: ECO:0005568 name: steady state inactivation curve evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:50:45Z [Term] id: ECO:0005569 name: window current trace evidence is_obsolete: true created_by: snadendla creation_date: 2016-02-09T12:57:16Z [Term] id: ECO:0005570 name: use dependence of inactivation evidence def: "A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course." [ECO:SN, url:http\://circres.ahajournals.org/content/89/8/700.full] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-09T12:58:18Z [Term] id: ECO:0005571 name: current clamp recording evidence def: "A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell." [ECO:SN, url:http\://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html] synonym: "current clamp voltage recording evidence" EXACT [] xref: birnlex:2278 "current clamp voltage recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T13:58:32Z [Term] id: ECO:0005572 name: whole-cell voltage clamp recording evidence def: "A type of voltage clamp recording evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage." [ECO:SN, url:http\://circres.ahajournals.org/content/72/1/91.full.pdf] xref: birnlex:2281 "whole-cell voltage clamp recording protocol" is_a: ECO:0005576 ! voltage clamp recording evidence created_by: snadendla creation_date: 2016-02-12T14:06:40Z [Term] id: ECO:0005573 name: cell-attached single-channel recording evidence def: "A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction." [ECO:SN, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf] synonym: "cell-attached patch-clamp recording evidence" EXACT [] xref: birnlex:2283 "cell-attached single-channel recording protocol" is_a: ECO:0006012 ! patch-clamp recording evidence created_by: snadendla creation_date: 2016-02-12T14:07:39Z [Term] id: ECO:0005574 name: cell-detached inside-out single-channel recording evidence def: "A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution." [ECO:SN, url:http\://www.acnp.org/g4/GN401000005/CH005.html] synonym: "cell-detached inside-out patch-clamp recording evidence" EXACT [] xref: birnlex:2286 "cell-detached inside-out single-channel recording protocol" is_a: ECO:0006012 ! patch-clamp recording evidence created_by: snadendla creation_date: 2016-02-12T14:16:53Z [Term] id: ECO:0005575 name: reconstituted bilayer single-channel patch recording evidence def: "A type of patch-clamp reocrding evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane." [ECO:SN, url:http\://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf] xref: birnlex:2287 "reconstituted bilayer single-channel patch recording protocol" is_a: ECO:0006012 ! patch-clamp recording evidence created_by: snadendla creation_date: 2016-02-12T14:17:46Z [Term] id: ECO:0005576 name: voltage clamp recording evidence def: "A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage." [ECO:SN, url:http\://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf, url:http\://www.acnp.org/g4/GN401000005/CH005.html] synonym: "voltage clamp current recording evidence" EXACT [] xref: birnlex:2279 "voltage clamp current recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:18:36Z [Term] id: ECO:0005577 name: electroencephalography recording evidence def: "A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head." [ECO:SN, url:http\://www.jove.com/science-education/5420/electro-encephalography-eeg] xref: birnlex:2293 "electroencephalography recording protocol" is_a: ECO:0000164 ! electrophysiology assay evidence created_by: snadendla creation_date: 2016-02-12T14:19:21Z [Term] id: ECO:0005578 name: super-resolution microscopy evidence def: "A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures." [ECO:SN, url:http\://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-02-12T15:20:47Z [Term] id: ECO:0005579 name: immunogold labelling evidence used in manual assertion def: "A type of immunogold labelling evidence that is used in a manual assertion." [ECO:SN] synonym: "immunogold labelling" BROAD [] is_a: ECO:0000090 ! immunogold labelling evidence is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion intersection_of: ECO:0000090 ! immunogold labelling evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-02-12T15:38:03Z [Term] id: ECO:0005580 name: flow cytometry evidence used in manual assertion def: "A type of flow cytometry evidence that is used in a manual assertion." [ECO:SN] synonym: "FCM" BROAD [] is_a: ECO:0000268 ! flow cytometry evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000268 ! flow cytometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-02-12T16:04:23Z [Term] id: ECO:0005581 name: enzyme-linked immunoabsorbent assay evidence used in manual assertion def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion." [ECO:SN] synonym: "ELISA evidence|enzyme-linked immunosorbent assay evidence" BROAD [] is_a: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-02-12T16:23:38Z [Term] id: ECO:0005582 name: cell-detached outside-out single-channel recording evidence def: "A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch)." [ECO:SN, url:http\://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html] synonym: "cell-detached outside-out patch-clamp evidence" EXACT [] is_a: ECO:0006012 ! patch-clamp recording evidence created_by: snadendla creation_date: 2016-03-02T10:47:25Z [Term] id: ECO:0005583 name: cut-open oocyte voltage clamp recording evidence def: "A type of voltage clamp recording evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway." [ECO:SN, PMID:9711615] is_a: ECO:0005576 ! voltage clamp recording evidence created_by: snadendla creation_date: 2016-03-02T11:29:32Z [Term] id: ECO:0005584 name: macropatch voltage clamp recording evidence def: "A type of voltage clamp recording evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch)." [ECO:SN, url:http\://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf] is_a: ECO:0005576 ! voltage clamp recording evidence created_by: snadendla creation_date: 2016-03-02T11:33:48Z [Term] id: ECO:0005585 name: high throughput mass spectrometry evidence def: "A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies." [ECO:SN, PMID:20805795] comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec.\nFor HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead. is_a: ECO:0001096 ! mass spectrometry evidence created_by: snadendla creation_date: 2016-03-02T12:33:07Z [Term] id: ECO:0005586 name: high throughput mass spectrometry evidence used in manual assertion def: "A type of high throughput mass spectrometry evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0005585 ! high throughput mass spectrometry evidence intersection_of: ECO:0005585 ! high throughput mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-03-02T12:50:02Z [Term] id: ECO:0005587 name: confocal microscopy evidence def: "A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-03-17T12:29:45Z [Term] id: ECO:0005588 name: wide-field microscopy evidence def: "A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times." [ECO:SN, url:http\://jcb.rupress.org/content/172/1/9.full, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/, url:http\://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html] is_a: ECO:0001098 ! microscopy evidence created_by: snadendla creation_date: 2016-03-17T12:30:27Z [Term] id: ECO:0005589 name: confocal microscopy evidence used in manual assertion def: "A type confocal microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005587 ! confocal microscopy evidence intersection_of: ECO:0005587 ! confocal microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-17T12:32:24Z [Term] id: ECO:0005590 name: wide-field microscopy evidence used in manual assertion def: "A type of wide-field microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005588 ! wide-field microscopy evidence intersection_of: ECO:0005588 ! wide-field microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-17T12:34:42Z [Term] id: ECO:0005591 name: immunogold labelling electron microscopy assay evidence def: "A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles." [ECO:SN, PMID:4110101] is_a: ECO:0000090 ! immunogold labelling evidence is_a: ECO:0005033 ! electron microscopy evidence created_by: snadendla creation_date: 2016-03-17T15:43:00Z [Term] id: ECO:0005592 name: immunogold labelling electron microscopy assay evidence used in manual assertion def: "A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005579 {is_inferred="true"} ! immunogold labelling evidence used in manual assertion is_a: ECO:0005591 ! immunogold labelling electron microscopy assay evidence is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion intersection_of: ECO:0005591 ! immunogold labelling electron microscopy assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-17T16:39:37Z [Term] id: ECO:0005593 name: immunodetection assay evidence def: "A type of direct assay evidence that involves the use of antibodies to detect biomolecules." [ECO:SN] is_a: ECO:0000002 ! direct assay evidence created_by: snadendla creation_date: 2016-03-17T17:07:33Z [Term] id: ECO:0005594 name: immunoperoxidase immunolocalization evidence def: "A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody." [ECO:SN] is_a: ECO:0000087 ! immunolocalization evidence created_by: snadendla creation_date: 2016-03-17T17:11:34Z [Term] id: ECO:0005595 name: immunoperoxidase immunolocalization evidence used in manual assertion def: "A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion intersection_of: ECO:0005594 ! immunoperoxidase immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-17T17:14:05Z [Term] id: ECO:0005596 name: immunoperoxidase immunolocalization electron microscopy evidence def: "A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody." [ECO:SN] synonym: "Immunoperoxidase labelling electron microscopy" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence created_by: snadendla creation_date: 2016-03-17T17:16:38Z [Term] id: ECO:0005597 name: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "immunoperoxidase labelling electron microscopy evidence" BROAD [] is_a: ECO:0005595 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence used in manual assertion is_a: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion intersection_of: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-17T17:23:56Z [Term] id: ECO:0005598 name: wide-field fluorescence microscopy evidence def: "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained. is_a: ECO:0005588 ! wide-field microscopy evidence created_by: snadendla creation_date: 2016-03-18T11:53:56Z [Term] id: ECO:0005599 name: immunofluorescence wide-field microscopy evidence def: "A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] synonym: "wide-field epifluorescence microscopy" EXACT [] is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence created_by: snadendla creation_date: 2016-03-18T11:55:40Z [Term] id: ECO:0005600 name: immunofluorescence confocal microscopy evidence def: "A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks/confocal/, url:https\://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf] comment: This allows visualization of sub cellular distribution of biomolecules of interest. is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0005587 ! confocal microscopy evidence property_value: IAO:0000112 "The endogenous protein expression and localization for WalR and WalK was also checked using confocal immunofluorescence microscopy. WalR could be localized to the cytoplasm of B. anthracis but WalK could not be detected once again (data not shown)." xsd:string {xref="PMID:24490131"} created_by: snadendla creation_date: 2016-03-18T12:48:42Z [Term] id: ECO:0005601 name: immunofluorescence confocal microscopy evidence used in manual assertion def: "A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion is_a: ECO:0005600 ! immunofluorescence confocal microscopy evidence is_a: ECO:0005804 {is_inferred="true"} ! immunofluorescence evidence used in manual assertion intersection_of: ECO:0005600 ! immunofluorescence confocal microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-03-18T12:52:04Z [Term] id: ECO:0005603 name: protein mass spectrometry evidence def: "A type of mass spectrometry evidence where one or more protein analyses are performed." [ECO:SN] is_a: ECO:0001096 ! mass spectrometry evidence created_by: snadendla creation_date: 2016-03-18T13:11:37Z [Term] id: ECO:0005604 name: cross-streak test evidence def: "A type of cell proliferation assay evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics." [ECO:SN, url:www.jstor.org/stable/3792785] is_a: ECO:0005007 ! cell proliferation assay evidence created_by: snadendla creation_date: 2016-03-29T15:40:54Z [Term] id: ECO:0005605 name: disk diffusion test evidence def: "A type of zone of inhibition evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism." [ECO:SN, url:http\://www.cdc.gov/nczved/resources/cholera/ch9.pdf] is_a: ECO:0007083 ! zone of inhibition evidence property_value: IAO:0000112 "Disk diffusion assays were used to determine if ohr and ohrR mutations affected resistance to ROS. The ohr mutant was less resistant than its parental strain when challenged with organic peroxides as shown by the zones of growth inhibition: 4.1 +- 0.2 cm for CuOOH and 3.1 +- 0.1 cm for tBOOH versus to 2.3 +- 0.2 and 2.5 +- 0.3 cm for wild type strain." xsd:string {xref="PMID:21569462"} created_by: snadendla creation_date: 2016-03-29T16:02:22Z [Term] id: ECO:0005606 name: cell transfection experiment evidence def: "A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function." [ECO:SN, url:http\://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/] is_a: ECO:0000006 ! experimental evidence created_by: snadendla creation_date: 2016-03-29T16:59:17Z [Term] id: ECO:0005607 name: tethered cell assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by observing the rotational behavior of the cells tethered to a coverslip by their flagella." [ECO:SN, PMID:1103143] is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T15:51:10Z [Term] id: ECO:0005608 name: tumble frequency assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another." [ECO:SN, PMID:4576832] synonym: "Temporal assay" EXACT [] is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T16:42:35Z [Term] id: ECO:0005609 name: capillary assay evidence def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the rate of accumulation of the cells into a capillary." [ECO:SN, PMID:4632978] comment: To determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured. comment: To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary. is_a: ECO:0005021 ! chemotaxis assay evidence created_by: snadendla creation_date: 2016-03-31T16:45:29Z [Term] id: ECO:0005610 name: biological system reconstruction evidence based on homology evidence used in manual assertion def: "A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion." [ECO:SN] comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence is_a: ECO:0007746 {is_inferred="true"} ! biological system reconstruction evidence used in manual assertion intersection_of: ECO:0005549 ! biological system reconstruction evidence based on homology evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISS" xsd:string created_by: snadendla creation_date: 2016-03-31T17:00:03Z [Term] id: ECO:0005611 name: inference from experimental data evidence def: "A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease." [ECO:SN] synonym: "IED" EXACT [] synonym: "inferred from experimental data" EXACT [] is_obsolete: true created_by: snadendla creation_date: 2016-04-12T16:55:16Z [Term] id: ECO:0005612 name: inference from phenotype manipulation evidence def: "A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined." [ECO:SN] comment: Changes can include any gene mutation/knockout, over-expression or ectopic expression of wild-type or mutant genes, anti-sense experiments, RNAi experiments, specific protein inhibitors. synonym: "inferred from phenotype manipulation" EXACT [] synonym: "IPM" EXACT [] is_obsolete: true created_by: snadendla creation_date: 2016-04-12T17:09:43Z [Term] id: ECO:0005613 name: inference by association of genotype from phenotype def: "A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments." [ECO:SN] comment: Used for 1) polymorphism or segregation of genetic markers (SNPs, mutations, RFLPs, microsatellites); 2) polymorphism or segregation of physical markers (FISH, centromeric or heterochromatic regions, chromosomal banding patterns); 3) detection of polymorphisms in inbred stock. synonym: "IAGP" EXACT [] synonym: "inferred by association of genotype from phenotype" EXACT [] is_a: ECO:0001237 ! natural variation mutant evidence used in manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-04-12T17:24:00Z [Term] id: ECO:0005614 name: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] synonym: "2D-PAGE evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence intersection_of: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-05-06T11:03:08Z [Term] id: ECO:0005615 name: alkaline phosphatase reporter gene assay evidence used in manual assertion def: "A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] synonym: "SEAP reporter assay" RELATED [] synonym: "secreted embryonic alkaline phosphatase reporter assay" RELATED [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence intersection_of: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T11:05:59Z [Term] id: ECO:0005616 name: beta-galactosidase reporter gene assay evidence used in manual assertion def: "A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] synonym: "beta-gal reporter gene assay evidence" BROAD [] synonym: "lacZ reporter gene assay" RELATED [] synonym: "LacZ transcript localization evidence used in manual assertion" EXACT [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001802 ! beta-galactosidase reporter gene assay evidence intersection_of: ECO:0001802 ! beta-galactosidase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T11:08:57Z [Term] id: ECO:0005617 name: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] synonym: "CAT reporter gene assay evidence" BROAD [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence intersection_of: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T11:11:07Z [Term] id: ECO:0005618 name: chromatin immunoprecipitation-chip evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion." [ECO:SN] synonym: "ChIP-chip evidence" BROAD [] synonym: "ChIP-on-chip evidence" BROAD [] is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T11:17:04Z [Term] id: ECO:0005619 name: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion." [ECO:SN] synonym: "ChIP-exo evidence" BROAD [] is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T11:22:25Z [Term] id: ECO:0005620 name: chromatin immunoprecipitation-PCR evidence used in manual assertion def: "A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion." [ECO:SN] synonym: "ChIP-PCR evidence" BROAD [] is_a: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T11:25:01Z [Term] id: ECO:0005621 name: chromatin immunoprecipitation-seq evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion." [ECO:SN] synonym: "ChIP-SEQ evidence" BROAD [] synonym: "ChIP-seq evidence" BROAD [] is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T11:29:13Z [Term] id: ECO:0005622 name: comparative genomics motif search evidence used in manual assertion def: "A type of comparative genomics motif search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005534 ! comparative genomics motif search evidence intersection_of: ECO:0005534 ! comparative genomics motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: snadendla creation_date: 2016-05-06T14:49:36Z [Term] id: ECO:0005623 name: comparative genomics motif search evidence used in automatic assertion def: "A type of comparative genomics motif search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005534 ! comparative genomics motif search evidence intersection_of: ECO:0005534 ! comparative genomics motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T14:52:21Z [Term] id: ECO:0005624 name: consensus search evidence used in manual assertion def: "A type of consensus search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005532 ! consensus search evidence intersection_of: ECO:0005532 ! consensus search evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: snadendla creation_date: 2016-05-06T14:54:53Z [Term] id: ECO:0005625 name: consensus search evidence used in automatic assertion def: "A type of consensus search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005532 ! consensus search evidence intersection_of: ECO:0005532 ! consensus search evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T14:58:22Z [Term] id: ECO:0005626 name: copper-phenanthroline footprinting evidence used in manual assertion def: "A type of copper-phenanthroline footprinting evidence that is used in a manual assertion." [ECO:SN] synonym: "1,10-phenanthroline-copper footprinting evidence" BROAD [] synonym: "OP-Cu Complex" RELATED [] is_a: ECO:0001815 ! copper-phenanthroline footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001815 ! copper-phenanthroline footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T15:01:25Z [Term] id: ECO:0005627 name: DNA adenine methyltransferase identification evidence used in manual assertion def: "A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion." [ECO:SN] synonym: "DamID evidence" BROAD [] is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T15:05:14Z [Term] id: ECO:0005628 name: DNA adenine methyltransferase identification evidence used in automatic assertion def: "A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion." [ECO:SN] synonym: "DamID evidence" BROAD [] is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T15:06:46Z [Term] id: ECO:0005629 name: DNA affinity chromatography evidence used in manual assertion def: "A type of DNA affinity chromatography evidence that is used in a manual assertion." [ECO:SN] synonym: "DNA affinity purification evidence" BROAD [] is_a: ECO:0001809 ! DNA affinity chromatography evidence is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion intersection_of: ECO:0001809 ! DNA affinity chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-06T15:08:40Z [Term] id: ECO:0005630 name: cDNA to DNA expression microarray evidence used in automatic assertion def: "A type of cDNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:SN] synonym: "DNA microarray evidence" BROAD [] synonym: "RNA microarray evidence" BROAD [] is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion intersection_of: ECO:0000097 ! cDNA to DNA expression microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T15:31:51Z [Term] id: ECO:0005631 name: DNAse footprinting evidence used in manual assertion def: "A type of DNAse footprinting evidence that is used in a manual assertion." [ECO:SN] synonym: "DNase protection evidence" BROAD [] is_a: ECO:0001810 ! DNAse footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001810 ! DNAse footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T15:35:41Z [Term] id: ECO:0005632 name: fluorescence anisotropy evidence used in manual assertion def: "A type of fluorescence anisotropy evidence that is used in a manual assertion." [ECO:SN] synonym: "FA evidence" BROAD [] synonym: "fluorescence polarization" RELATED [] synonym: "FP" RELATED [] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0001811 ! fluorescence anisotropy evidence intersection_of: ECO:0001811 ! fluorescence anisotropy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-05-06T15:45:40Z [Term] id: ECO:0005633 name: ferric uptake regulator titration assay evidence used in manual assertion def: "A type of ferric uptake regulator titration assay evidence that is used in a manual assertion." [ECO:SN] synonym: "FURTA evidence" BROAD [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001829 ! ferric uptake regulator titration assay evidence intersection_of: ECO:0001829 ! ferric uptake regulator titration assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T15:49:18Z [Term] id: ECO:0005634 name: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion." [ECO:SN] synonym: "genomic SELEX evidence" BROAD [] is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence is_a: ECO:0006088 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence used in manual assertion intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T16:41:30Z [Term] id: ECO:0005635 name: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:SN] synonym: "genomic SELEX evidence" BROAD [] is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence is_a: ECO:0007510 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence used in automatic assertion intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T16:43:03Z [Term] id: ECO:0005636 name: green fluorescent protein reporter gene assay evidence used in manual assertion def: "A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion." [ECO:SN] synonym: "GFP promoter fusion" RELATED [] synonym: "GFP reporter gene assay evidence" BROAD [] synonym: "green fluorescent protein promoter fusion" RELATED [] synonym: "green fluorescent protein transcript localization evidence used in manual assertion" EXACT [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T16:46:32Z [Term] id: ECO:0005637 name: green fluorescent protein reporter gene assay evidence used in automatic assertion def: "A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] synonym: "GFP promoter fusion" RELATED [] synonym: "GFP reporter gene assay evidence" BROAD [] synonym: "green fluorescent protein promoter fusion" RELATED [] synonym: "green fluorescent protein transcript localization evidence used in automatic assertion" EXACT [] is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T16:48:29Z [Term] id: ECO:0005638 name: cell growth regulation assay evidence used in manual assertion def: "A type of cell growth regulation assay evidence that is used in a manual assertion." [ECO:SN] synonym: "growth curve analysis" RELATED [] is_a: ECO:0000095 ! cell growth regulation assay evidence is_a: ECO:0007699 {is_inferred="true"} ! cell growth assay evidence used in manual assertion intersection_of: ECO:0000095 ! cell growth regulation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: snadendla creation_date: 2016-05-06T16:52:15Z [Term] id: ECO:0005639 name: cell growth regulation assay evidence used in automatic assertion def: "A type of cell growth regulation assay evidence that is used in an automatic assertion." [ECO:SN] synonym: "growth curve analysis" RELATED [] is_a: ECO:0000095 ! cell growth regulation assay evidence is_a: ECO:0007778 {is_inferred="true"} ! cell growth assay evidence used in automatic assertion intersection_of: ECO:0000095 ! cell growth regulation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T16:53:28Z [Term] id: ECO:0005640 name: glutathione S-transferase pull-down assay evidence used in manual assertion def: "A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion." [ECO:SN] synonym: "GST pull-down assay evidence" BROAD [] is_a: ECO:0001817 ! glutathione S-transferase pull-down assay evidence is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion intersection_of: ECO:0001817 ! glutathione S-transferase pull-down assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T16:56:39Z [Term] id: ECO:0005641 name: beta-glucuronidase reporter gene assay evidence used in manual assertion def: "A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] synonym: "GUS reporter gene assay evidence" BROAD [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence intersection_of: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T16:58:29Z [Term] id: ECO:0005642 name: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "HSQC evidence" BROAD [] is_a: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion intersection_of: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-06T23:10:13Z [Term] id: ECO:0005643 name: hydroxyl-radical footprinting evidence used in manual assertion def: "A type of hydroxyl-radical footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001818 ! hydroxyl-radical footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001818 ! hydroxyl-radical footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T23:14:48Z [Term] id: ECO:0005644 name: immunoprecipitation evidence used in manual assertion def: "A type of immunoprecipitation evidence that is used in a manual assertion." [ECO:SN] synonym: "immunoprecipitation" BROAD [] is_a: ECO:0000085 ! immunoprecipitation evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion intersection_of: ECO:0000085 ! immunoprecipitation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T23:20:49Z [Term] id: ECO:0005645 name: interferometric reflectance imaging sensor evidence used in manual assertion def: "A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion." [ECO:SN] synonym: "IRIS evidence" BROAD [] synonym: "spectral reflectance imaging biosensor evidence" BROAD [] synonym: "SRIB evidence" BROAD [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-06T23:27:58Z [Term] id: ECO:0005646 name: interferometric reflectance imaging sensor evidence used in automatic assertion def: "A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion." [ECO:SN] synonym: "IRIS evidence" BROAD [] synonym: "spectral reflectance imaging biosensor evidence" BROAD [] synonym: "SRIB evidence" BROAD [] is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T23:29:23Z [Term] id: ECO:0005647 name: isothermal titration calorimetry evidence used in manual assertion def: "A type of isothermal titration calorimetry evidence that is used in a manual assertion." [ECO:SN] synonym: "ITC evidence" BROAD [] is_a: ECO:0001825 ! isothermal titration calorimetry evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0001825 ! isothermal titration calorimetry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-06T23:36:26Z [Term] id: ECO:0005648 name: luciferase reporter gene assay evidence used in manual assertion def: "A type of luciferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001805 ! luciferase reporter gene assay evidence intersection_of: ECO:0001805 ! luciferase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-06T23:44:52Z [Term] id: ECO:0005649 name: machine learning prediction of motif instance evidence used in manual assertion def: "A type of machine learning prediction of motif instance evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion is_a: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: snadendla creation_date: 2016-05-06T23:51:30Z [Term] id: ECO:0005650 name: machine learning prediction of motif instance evidence used in automatic assertion def: "A type of machine learning prediction of motif instance evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion is_a: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T23:54:00Z [Term] id: ECO:0005651 name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] synonym: "MALDI-TOF-MS evidence" BROAD [] is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-06T23:57:30Z [Term] id: ECO:0005652 name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion." [ECO:SN] synonym: "Methidiumpropyl-EDTA Fe(II) footprinting evidence" BROAD [] synonym: "MPE Fe(II) footprinting" RELATED [] synonym: "MPE-EDTA Fe(II) footprinting evidence" BROAD [] is_a: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-09T14:42:28Z [Term] id: ECO:0005653 name: northern blot evidence used in manual assertion def: "A type of northern blot evidence that is used in a manual assertion." [ECO:SN] synonym: "northern assay evidence" BROAD [] synonym: "RNA blot evidence" BROAD [] synonym: "transcript levels (e.g. Northerns)" RELATED [] is_a: ECO:0000106 ! northern blot evidence is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion intersection_of: ECO:0000106 ! northern blot evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-09T14:48:03Z [Term] id: ECO:0005654 name: phylogenetic footprinting evidence used in manual assertion def: "A type of phylogenetic footprinting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005533 ! phylogenetic footprinting evidence is_a: ECO:0005558 {is_inferred="true"} ! motif discovery evidence used in manual assertion intersection_of: ECO:0005533 ! phylogenetic footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: snadendla creation_date: 2016-05-09T14:50:52Z [Term] id: ECO:0005655 name: phylogenetic footprinting evidence used in automatic assertion def: "A type of phylogenetic footprinting evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005533 ! phylogenetic footprinting evidence is_a: ECO:0005559 {is_inferred="true"} ! motif discovery evidence used in automatic assertion intersection_of: ECO:0005533 ! phylogenetic footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-09T14:51:55Z [Term] id: ECO:0005656 name: methylation interference footprinting evidence used in manual assertion def: "A type of methylation interference footprinting evidence that is used in a manual assertion." [ECO:SN] synonym: "premethylation interference footprinting evidence used in manual assertion" EXACT [] is_a: ECO:0001827 ! methylation interference footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001827 ! methylation interference footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-09T14:55:25Z [Term] id: ECO:0005657 name: primer extension assay evidence used in manual assertion def: "A type of primer extension assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0001819 ! primer extension assay evidence intersection_of: ECO:0001819 ! primer extension assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-09T14:58:38Z [Term] id: ECO:0005658 name: position-specific scoring matrix motif search evidence used in manual assertion def: "A type of position-specific scoring matrix motif search evidence that is used in a manual assertion." [ECO:SN] synonym: "PSSM motif search evidence" BROAD [] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: snadendla creation_date: 2016-05-09T15:01:31Z [Term] id: ECO:0005659 name: position-specific scoring matrix motif search evidence used in automatic assertion def: "A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion." [ECO:SN] synonym: "PSSM motif search evidence" BROAD [] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-09T15:02:31Z [Term] id: ECO:0005660 name: quantitative polymerase chain reaction evidence used in manual assertion def: "A type of quantitative polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000231 ! quantitative polymerase chain reaction evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000231 ! quantitative polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-09T15:09:01Z [Term] id: ECO:0005661 name: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] synonym: "RACE PCR evidence" BROAD [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence intersection_of: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-09T15:19:20Z [Term] id: ECO:0005662 name: regular expression motif search evidence used in manual assertion def: "A type of regular expression motif search evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion is_a: ECO:0005505 ! regular expression motif search evidence intersection_of: ECO:0005505 ! regular expression motif search evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISM" xsd:string created_by: snadendla creation_date: 2016-05-09T16:13:55Z [Term] id: ECO:0005663 name: regular expression motif search evidence used in automatic assertion def: "A type of regular expression motif search evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion is_a: ECO:0005505 ! regular expression motif search evidence intersection_of: ECO:0005505 ! regular expression motif search evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-09T16:15:03Z [Term] id: ECO:0005664 name: RNA sequencing assay evidence used in manual assertion comment: Use ECO:0006068 (RNA-seq evidence used in manual assertion) in place of this term. is_obsolete: true created_by: snadendla creation_date: 2016-05-09T16:18:35Z [Term] id: ECO:0005665 name: RNA sequencing assay evidence used in automatic assertion comment: Use ECO:0006069 (RNA-seq evidence used in automatic assertion) in place of this term. is_obsolete: true created_by: snadendla creation_date: 2016-05-09T16:19:38Z [Term] id: ECO:0005666 name: S1 nuclease protection assay evidence used in manual assertion def: "A type of S1 nuclease protection assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion is_a: ECO:0005521 ! S1 nuclease protection assay evidence intersection_of: ECO:0005521 ! S1 nuclease protection assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-09T16:22:58Z [Term] id: ECO:0005667 name: site-directed mutagenesis phenotypic evidence used in manual assertion def: "A type of site-directed phenotypic mutagenesis evidence that is used in a manual assertion." [ECO:SN] synonym: "site directed mutagenesis evidence" BROAD [] synonym: "site-directed mutagenesis evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005528 ! site-directed mutagenesis phenotypic evidence intersection_of: ECO:0005528 ! site-directed mutagenesis phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: snadendla creation_date: 2016-05-09T16:29:13Z [Term] id: ECO:0005668 name: survival rate analysis evidence used in manual assertion def: "A type of survival rate analysis evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion is_a: ECO:0001822 ! survival rate analysis evidence intersection_of: ECO:0001822 ! survival rate analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: snadendla creation_date: 2016-05-09T16:31:08Z [Term] id: ECO:0005669 name: ultraviolet light footprinting evidence used in manual assertion def: "A type of ultraviolet light footprinting evidence that is used in a manual assertion." [ECO:SN] synonym: "UV footprinting" RELATED [] is_a: ECO:0001826 ! ultraviolet light footprinting evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001826 ! ultraviolet light footprinting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: snadendla creation_date: 2016-05-09T16:33:44Z [Term] id: ECO:0005670 name: x-ray crystallography evidence used in manual assertion def: "A type of x-ray crystallography evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001171 {is_inferred="true"} ! crystallography evidence used in manual assertion is_a: ECO:0001823 ! x-ray crystallography evidence intersection_of: ECO:0001823 ! x-ray crystallography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-10T11:53:00Z [Term] id: ECO:0005671 name: x-ray crystallography evidence used in automatic assertion def: "A type of x-ray crystallography evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0001823 ! x-ray crystallography evidence is_a: ECO:0007374 {is_inferred="true"} ! crystallography evidence used in automatic assertion intersection_of: ECO:0001823 ! x-ray crystallography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla creation_date: 2016-05-10T11:54:03Z [Term] id: ECO:0005672 name: xylE reporter gene assay evidence used in manual assertion def: "A type of xylE reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0005537 ! xylE reporter gene assay evidence intersection_of: ECO:0005537 ! xylE reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla creation_date: 2016-05-10T11:57:15Z [Term] id: ECO:0005673 name: ad-hoc qualitative phenotype observation evidence def: "A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2016-05-10T12:57:41Z [Term] id: ECO:0005674 name: ad-hoc qualitative phenotype observation evidence used in manual assertion def: "A type of ad-hoc qualitative phenotype observation that is used in a manual assertion." [ECO:SN] is_a: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-10T14:28:47Z [Term] id: ECO:0005675 name: ad-hoc quantitative phenotype observation evidence def: "A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 "CollecTF" xsd:string created_by: snadendla creation_date: 2016-05-10T14:30:50Z [Term] id: ECO:0005676 name: ad-hoc quantitative phenotype observation evidence used in manual assertion def: "A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: snadendla creation_date: 2016-05-10T14:34:50Z [Term] id: ECO:0005800 name: dilution assay evidence def: "A type of direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." [http://www.sciencedirect.com/science/article/pii/016770129400068I] comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay. synonym: "critical dilution assay" EXACT [] synonym: "spot assay" EXACT [] is_a: ECO:0000002 ! direct assay evidence [Term] id: ECO:0005801 name: enzymatic activity assay evidence used in manual assertion def: "A type of enzyme assay evidence that is used in a manual assertion." [ECO:SN] synonym: "enzyme assays" BROAD [] is_a: ECO:0000005 ! enzymatic activity assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000005 ! enzymatic activity assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string [Term] id: ECO:0005802 name: cell transfection experiment evidence used in manual assertion def: "A type of cell transfection experiment evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0005606 ! cell transfection experiment evidence intersection_of: ECO:0005606 ! cell transfection experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string [Term] id: ECO:0005803 name: motility assay evidence def: "A type of direct assay evidence where the motility of a cell or cells is determined." [ECO:SN] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000112 "A motility assay at 37 degrees C revealed a non-motile phenotype for all strains, as expected (data not shown)." xsd:string {xref="PMID:20830609"} [Term] id: ECO:0005804 name: immunofluorescence evidence used in manual assertion def: "A type of immunofluorescence evidence that is used in a manual assertion." [ECO:SN] synonym: "immunofluorescence" BROAD [] is_a: ECO:0000007 ! immunofluorescence evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000007 ! immunofluorescence evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string [Term] id: ECO:0005805 name: yeast 2-hybrid evidence used in manual assertion def: "A type of yeast 2-hybrid evidence that is used in a manual assertion." [ECO:SN] synonym: "yeast two-hybrid assay" BROAD [] is_a: ECO:0000068 ! yeast 2-hybrid evidence is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion intersection_of: ECO:0000068 ! yeast 2-hybrid evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string [Term] id: ECO:0006001 name: Cya fusion reporter assay evidence def: "A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications." [PMID:10217833, PMID:14702323] is_a: ECO:0000017 ! ectopic expression evidence is_a: ECO:0000049 ! reporter gene assay evidence created_by: rctauber creation_date: 2016-06-15T08:42:16Z [Term] id: ECO:0006002 name: Cya fusion reporter assay evidence used in manual assertion def: "A type of Cya fusion reporter assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0001273 {is_inferred="true"} ! ectopic expression evidence used in manual assertion is_a: ECO:0006001 ! Cya fusion reporter assay evidence intersection_of: ECO:0006001 ! Cya fusion reporter assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string property_value: IAO:0000234 "CollecTF" xsd:string created_by: rctauber creation_date: 2010-06-15T08:44:10Z [Term] id: ECO:0006003 name: electron microscopy evidence used in manual assertion def: "A type of electron microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005033 ! electron microscopy evidence intersection_of: ECO:0005033 ! electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006004 name: super-resolution microscopy evidence used in manual assertion def: "A type of super-resolution microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0005578 ! super-resolution microscopy evidence intersection_of: ECO:0005578 ! super-resolution microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006005 name: fractionation evidence used in manual assertion def: "A type of fractionation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000100 ! fractionation evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000100 ! fractionation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006006 name: electrophysiology assay evidence used in manual assertion def: "A type of electrophysiology assay evidence that is used in a manual assertion." [] {comment="ECO:RCT"} is_a: ECO:0000164 ! electrophysiology assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000164 ! electrophysiology assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-10-12T12:32:29Z [Term] id: ECO:0006007 name: chromatin immunoprecipitation-chip evidence used in manual assertion def: "A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion." [ECO:RCT] synonym: "ChIP-chip evidence" BROAD [] synonym: "ChIP-on-chip evidence" BROAD [] is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006008 name: chromatin immunoprecipitation- exonuclease evidence used in manual assertion def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion." [ECO:RCT] synonym: "ChIP-exo" BROAD [] is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006009 name: chromatin immunoprecipitation-seq evidence used in manual assertion def: "A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion." [ECO:RCT] synonym: "ChIP-SEQ evidence" BROAD [] synonym: "ChIP-seq evidence" BROAD [] is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2016-10-21T11:55:53Z [Term] id: ECO:0006010 name: mRNA interactome capture evidence def: "A type of nucleic acid binding evidence in which RNA-binding proteins are identified through cross-linking RNA and proteins by UV light, capturing RNA-protein complexes on oligo(dT) beads, and finally identifying by mass spectrometry." [PMID:27729395] synonym: "ultraviolet light cross-linking RNA binding evidence" EXACT [] is_a: ECO:0000136 ! nucleic acid binding evidence created_by: rctauber creation_date: 2016-10-21T12:28:17Z [Term] id: ECO:0006011 name: mRNA interactome capture evidence used in manual assertion def: "A type of mRNA interactome capture evidence that is used in a manual assertion." [ECO:RCT] synonym: "ultraviolet light cross-linking RNA binding evidence" BROAD [] is_a: ECO:0006010 ! mRNA interactome capture evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0006010 ! mRNA interactome capture evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2016-10-21T12:28:17Z [Term] id: ECO:0006012 name: patch-clamp recording evidence def: "A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels." [ECO:RCT] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: rctauber creation_date: 2016-11-28T11:44:05Z [Term] id: ECO:0006013 name: patch-clamp recording evidence used in manual assertion def: "A type of patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion is_a: ECO:0006012 ! patch-clamp recording evidence intersection_of: ECO:0006012 ! patch-clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-11-28T11:44:05Z [Term] id: ECO:0006014 name: whole-cell patch-clamp recording evidence def: "A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell." [PMID:27341060] is_a: ECO:0006012 ! patch-clamp recording evidence created_by: rctauber creation_date: 2016-11-28T11:44:05Z [Term] id: ECO:0006015 name: whole-cell patch-clamp recording evidence used in manual assertion def: "A type of whole-cell patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion is_a: ECO:0006014 ! whole-cell patch-clamp recording evidence intersection_of: ECO:0006014 ! whole-cell patch-clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-11-28T11:44:05Z [Term] id: ECO:0006016 name: author statement from published clinical study def: "A type of traceable author statement that is based on a publication about a clinical study." [] synonym: "published clinical study evidence" EXACT [] synonym: "traceable author statement from published clinical study" RELATED [] is_a: ECO:0000033 ! author statement supported by traceable reference created_by: rctauber creation_date: 2016-11-30T09:44:47Z [Term] id: ECO:0006017 name: author statement from published clinical study used in manual assertion def: "A type of author statement from published clinical study that is used in a manual assertion." [] comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used when evidence for an HPO annotation is information extracted from articles in the medical literature. synonym: "PCS" EXACT [HPO:PCS] synonym: "published clinical study evidence" BROAD [] is_a: ECO:0000304 {is_inferred="true"} ! author statement supported by traceable reference used in manual assertion is_a: ECO:0006016 ! author statement from published clinical study intersection_of: ECO:0006016 ! author statement from published clinical study intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "TAS" xsd:string property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string created_by: rctauber creation_date: 2016-11-30T09:44:47Z [Term] id: ECO:0006018 name: inference based on individual clinical experience def: "A type of curator inference that is based on the individual clinical experience of a clinician" [] synonym: "individual clinical experience evidence" EXACT [] is_a: ECO:0000205 ! curator inference created_by: rctauber creation_date: 2016-11-30T09:44:47Z [Term] id: ECO:0006019 name: inference based on individual clinical experience used in manual assertion def: "A type of inference based on individual clinical experience that is used in a manual assertion." [] comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used for annotating disorders with a limited amount of published data, and is accompanied by a reference to the individual or center performing the annotation. synonym: "ICE" EXACT [HPO:ICE] synonym: "individual clinical experience evidence" BROAD [] is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion is_a: ECO:0006018 ! inference based on individual clinical experience intersection_of: ECO:0006018 ! inference based on individual clinical experience intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IC" xsd:string property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string created_by: rctauber creation_date: 2016-11-30T09:44:47Z [Term] id: ECO:0006020 name: biofilm formation assay evidence def: "A type of cell proliferation assay evidence in which biofilm growth is monitored and detected from attachment to development." [PMID:10547784] synonym: "biofilm assay evidence" EXACT [] is_a: ECO:0005007 ! cell proliferation assay evidence created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006021 name: biofilm formation assay evidence used in manual assertion def: "A type of biofilm formation assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006020 ! biofilm formation assay evidence is_a: ECO:0007696 {is_inferred="true"} ! cell proliferation assay evidence used in manual assertion intersection_of: ECO:0006020 ! biofilm formation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006022 name: microtiter plate biofilm assay evidence def: "A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes." [PMID:10547784] synonym: "96-well biofilm assay evidence" EXACT [] is_a: ECO:0006020 ! biofilm formation assay evidence created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006023 name: microtiter plate biofilm assay evidence used in manual assertion def: "A type of microtiter plate biofilm assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion is_a: ECO:0006022 ! microtiter plate biofilm assay evidence intersection_of: ECO:0006022 ! microtiter plate biofilm assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006024 name: air-liquid interface assay evidence def: "A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate." [PMID:18770545] comment: Tilting of the plate positions the air-liquid interface on a clear portion to improve visability. synonym: "ALI biofilm assay evidence" EXACT [] is_a: ECO:0006020 ! biofilm formation assay evidence created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006025 name: air-liquid interface assay evidence used in manual assertion def: "A type of air-liquid interface assay evidence that is used in a manual assertion." [ECO:RCT] synonym: "ALI biofilm assay evidence" BROAD [] is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion is_a: ECO:0006024 ! air-liquid interface assay evidence intersection_of: ECO:0006024 ! air-liquid interface assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006026 name: colony biofilm assay evidence def: "A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate. The plate serves to supply nutrients and the semipermeable membrane can be relocated to fresh plates." [PMID:18770545] comment: The plates commonly contain different carbon sources or antibiotic treatments to observe properties of the cells. is_a: ECO:0006020 ! biofilm formation assay evidence created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006027 name: colony biofilm assay evidence used in manual assertion def: "A type of colony biofilm assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion is_a: ECO:0006026 ! colony biofilm assay evidence intersection_of: ECO:0006026 ! colony biofilm assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006028 name: Kadouri drip-fed biofilm assay evidence def: "A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out." [PMID:18770545] is_a: ECO:0006020 ! biofilm formation assay evidence created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006029 name: Kadouri drip-fed biofilm assay evidence used in manual assertion def: "A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion is_a: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence intersection_of: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2016-12-05T09:33:18Z [Term] id: ECO:0006030 name: co-immunoprecipitation evidence used in manual assertion def: "A type of co-immunoprecipitation evidence that is used in a manual assertion." [ECO:RCT] synonym: "co-immunoprecipitation" BROAD [] is_a: ECO:0000070 ! co-immunoprecipitation evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000070 ! co-immunoprecipitation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2016-01-13T11:32:26Z [Term] id: ECO:0006031 name: immunolocalization evidence used in manual assertion def: "A type of immunolocalization evidence that is used in a manual assertion." [ECO:RCT] synonym: "immunolocalization" BROAD [] is_a: ECO:0000087 ! immunolocalization evidence is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion intersection_of: ECO:0000087 ! immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-01-23T07:54:37Z [Term] id: ECO:0006032 name: optogenetic evidence def: "A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators." [GOC:DOS, PMID:17035522] synonym: "optogenetic actuator evidence" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006033 name: optogenetic evidence used in manual assertion def: "A type of optogenetic evidence that is used in a manual assertion." [ECO:RCT] synonym: "optogenetic actuator evidence" BROAD [] is_a: ECO:0006032 ! optogenetic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0006032 ! optogenetic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006034 name: fluorescent sensor evidence def: "A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor." [GOC:DOS] comment: Examples include sensors for ion concentration and potential difference across a membrane. is_a: ECO:0001115 ! fluorescence evidence created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006035 name: fluorescent sensor evidence used in manual assertion def: "A type of fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006034 ! fluorescent sensor evidence intersection_of: ECO:0006034 ! fluorescent sensor evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006036 name: genetically encoded fluorescent sensor evidence def: "A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor." [GOC:DOS] synonym: "optogenetic sensor evidence" EXACT [] is_a: ECO:0006034 ! fluorescent sensor evidence created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006037 name: genetically encoded fluorescent sensor evidence used in manual assertion def: "A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] synonym: "optogenetic sensor evidence" BROAD [] is_a: ECO:0006035 {is_inferred="true"} ! fluorescent sensor evidence used in manual assertion is_a: ECO:0006036 ! genetically encoded fluorescent sensor evidence intersection_of: ECO:0006036 ! genetically encoded fluorescent sensor evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006038 name: genetically encoded fluorescent electrophysiology assay evidence def: "A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors." [GOC:DOS] comment: Examples include genetically encoded sensors that detect potential difference across plasma membrane. synonym: "electrophysiology - optical assay evidence" EXACT [] is_a: ECO:0000164 ! electrophysiology assay evidence is_a: ECO:0006034 ! fluorescent sensor evidence created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006039 name: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion." [ECO:RCT] synonym: "electrophysiology - optical assay evidence" BROAD [] is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion is_a: ECO:0006035 {is_inferred="true"} ! fluorescent sensor evidence used in manual assertion is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence intersection_of: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006040 name: genetically encoded fluorescent ion concentration sensor assay evidence def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor." [GOC:DOS] comment: Examples include the genetically encoded calcium indicator GCaMP. is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006041 name: genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion def: "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006039 {is_inferred="true"} ! genetically encoded fluorescent electrophysiology assay evidence used in manual assertion is_a: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence intersection_of: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-01-23T11:58:48Z [Term] id: ECO:0006042 name: cell fractionation evidence used in manual assertion def: "A type of cell fractionation evidence that is used in a manual assertion." [ECO:RCT] synonym: "cell fractionation" BROAD [] is_a: ECO:0000004 ! cell fractionation evidence is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion intersection_of: ECO:0000004 ! cell fractionation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006043 name: extracellular recording evidence def: "A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons." [url:http\://www.nature.com/subjects/extracellular-recording] is_a: ECO:0000164 ! electrophysiology assay evidence created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006044 name: extracellular recording evidence used in manual assertion def: "A type of extracellular recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion is_a: ECO:0006043 ! extracellular recording evidence intersection_of: ECO:0006043 ! extracellular recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006045 name: single-unit extracellular recording evidence def: "A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron." [doi:10.1385/0-89603-185-3\:1] is_a: ECO:0006043 ! extracellular recording evidence created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006046 name: single-unit extracellular recording evidence used in manual assertion def: "A type of single-unit extracellular recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006044 {is_inferred="true"} ! extracellular recording evidence used in manual assertion is_a: ECO:0006045 ! single-unit extracellular recording evidence intersection_of: ECO:0006045 ! single-unit extracellular recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006047 name: field potential recording evidence def: "A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes." [ECO:RCT] synonym: "local field potential recording evidence" NARROW [] is_a: ECO:0006043 ! extracellular recording evidence created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006048 name: field potential recording evidence used in manual assertion def: "A type of field potential recording evidence that is used in a manual assertion." [ECO:RCT] synonym: "local field potential recording evidence" RELATED [] is_a: ECO:0006044 {is_inferred="true"} ! extracellular recording evidence used in manual assertion is_a: ECO:0006047 ! field potential recording evidence intersection_of: ECO:0006047 ! field potential recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-02-21T07:26:28Z [Term] id: ECO:0006049 name: genetic transformation evidence used in manual assertion def: "A type of genetic transformation evidence that is used in a manual assertion" [ECO:RCT] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005027 ! genetic transformation evidence intersection_of: ECO:0005027 ! genetic transformation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2017-02-21T18:08:58Z [Term] id: ECO:0006050 name: anti-sense experiment evidence used in manual assertion def: "A type of anti-sense experiment evidence that is used in a manual assertion." [ECO:RCT] synonym: "anti-sense experiments" BROAD [] is_a: ECO:0000018 ! anti-sense experiment evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000018 ! anti-sense experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2017-02-21T18:08:58Z [Term] id: ECO:0006051 name: morpholino experiment evidence used in manual assertion def: "A type of morpholino experiment evidence that is used in a manual assertion." [ECO:RCT] synonym: "anti-sense evidence" BROAD [] is_a: ECO:0000292 ! morpholino experiment evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000292 ! morpholino experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2017-02-21T18:08:58Z [Term] id: ECO:0006052 name: RNAi evidence used in manual assertion def: "A type of RNAi evidence that is used in a manual assertion." [ECO:RCT] synonym: "RNAi experiment" BROAD [] is_a: ECO:0000019 ! RNAi evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000019 ! RNAi evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2017-02-21T18:08:58Z [Term] id: ECO:0006053 name: pharmacological assay evidence def: "A type of experimental phenotypic evidence that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] is_a: ECO:0000059 ! experimental phenotypic evidence created_by: dosumis creation_date: 2017-03-07T08:51:02Z [Term] id: ECO:0006054 name: pharmacological assay evidence used in manual assertion def: "A type of experimental phenotypic evidence used in a manual assertion that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] is_a: ECO:0006053 ! pharmacological assay evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0006053 ! pharmacological assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2017-03-07T08:51:02Z [Term] id: ECO:0006055 name: high throughput evidence def: "A type of evidence where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:MCC] comment: Some relevant articles include: PMID:23340846, doi:10.1038/nmeth0607-523 synonym: "high throughput cell biology evidence" NARROW [] synonym: "high throughput screening evidence" NARROW [] synonym: "high-throughput evidence" EXACT [] synonym: "HT evidence" EXACT [] is_a: ECO:0000000 ! evidence created_by: mchibucos creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006056 name: high throughput evidence used in manual assertion def: "A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel." [ECO:RCT] synonym: "high throughput cell biology evidence" RELATED [] synonym: "high throughput screening evidence" RELATED [] synonym: "high-throughput evidence" BROAD [] synonym: "HT evidence" BROAD [] synonym: "HTP" EXACT [GOECO:HTP] synonym: "inferred from high throughput experiment" EXACT [GOECO:HTP] xref: GOECO:HTP "inferred from high throughput experiment" is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0006055 ! high throughput evidence intersection_of: ECO:0006055 ! high throughput evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HTP" xsd:string {comment="Default"} created_by: rctauber creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006057 name: high throughput evidence used in automatic assertion def: "A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:RCT] synonym: "high throughput cell biology evidence" RELATED [] synonym: "high throughput screening evidence" RELATED [] synonym: "high-throughput evidence" BROAD [] synonym: "HT evidence" BROAD [] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0006055 ! high throughput evidence intersection_of: ECO:0006055 ! high throughput evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006058 name: high throughput cell biology evidence def: "A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:MCC] comment: HT methodologies may incorporate techniques from optics, chemistry, biology or image analysis (https://en.wikipedia.org/wiki/High_throughput_biology) synonym: "omics experiment" RELATED [] is_a: ECO:0006055 ! high throughput evidence created_by: mchibucos creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006059 name: high throughput cell biology evidence used in manual assertion def: "A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] synonym: "omics experiment" RELATED [] is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion is_a: ECO:0006058 ! high throughput cell biology evidence intersection_of: ECO:0006058 ! high throughput cell biology evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HTP" xsd:string created_by: rctauber creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006060 name: high throughput cell biology evidence used in automatic assertion def: "A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] synonym: "omics experiment" RELATED [] is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion is_a: ECO:0006058 ! high throughput cell biology evidence intersection_of: ECO:0006058 ! high throughput cell biology evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2017-03-28T14:48:39Z [Term] id: ECO:0006061 name: immunofluorescence wide-field microscopy evidence used in manual assertion def: "A type of wide-field fluorescence microscopy evidence used in a manual assertion where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] synonym: "wide-field epifluorescence microscopy evidence" BROAD [] is_a: ECO:0005599 ! immunofluorescence wide-field microscopy evidence is_a: ECO:0005804 {is_inferred="true"} ! immunofluorescence evidence used in manual assertion is_a: ECO:0006062 {is_inferred="true"} ! wide-field fluorescence microscopy evidence used in manual assertion intersection_of: ECO:0005599 ! immunofluorescence wide-field microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-05-15T08:57:07Z [Term] id: ECO:0006062 name: wide-field fluorescence microscopy evidence used in manual assertion def: "A type of wide-field microscopy evidence that is used in a manual assertion where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] is_a: ECO:0005590 {is_inferred="true"} ! wide-field microscopy evidence used in manual assertion is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence intersection_of: ECO:0005598 ! wide-field fluorescence microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-05-15T09:01:19Z [Term] id: ECO:0006063 name: over expression analysis evidence used in manual assertion def: "A type of experimental phenotypic evidence that is used in a manual assertion where a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] is_a: ECO:0000120 ! over expression analysis evidence is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion intersection_of: ECO:0000120 ! over expression analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2017-05-15T09:03:49Z [Term] id: ECO:0006064 name: cell-free assay evidence used in manual assertion def: "A type of cell-free assay evidence that is used in a manual assertion." [PMID:18453125] synonym: "in vitro assay evidence" BROAD [] is_a: ECO:0000183 ! cell-free assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000183 ! cell-free assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-05-15T09:08:17Z [Term] id: ECO:0006065 name: in vitro cell based assay evidence used in manual assertion comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. is_obsolete: true created_by: rctauber creation_date: 2017-05-15T09:11:13Z [Term] id: ECO:0006066 name: fluorescence recovery after photobleaching evidence def: "A type of fluorescence evidence where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [PMID:26314367] comment: FRAP is a fluorescence microscopy-based technique. {xref="PMID:26314367"} synonym: "FRAP evidence" EXACT [] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 "dosumis" xsd:string created_by: rctauber creation_date: 2017-05-16T10:55:40Z [Term] id: ECO:0006067 name: fluorescence recovery after photobleaching evidence used in manual assertion def: "A type of fluorescence evidence that is used in a manual assertion where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [] synonym: "FRAP evidence" BROAD [] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006066 ! fluorescence recovery after photobleaching evidence intersection_of: ECO:0006066 ! fluorescence recovery after photobleaching evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2017-05-16T10:55:40Z [Term] id: ECO:0006068 name: RNA-sequencing evidence used in manual assertion def: "A type of high throughput nucleotide sequencing assay evidence used in a manual assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] synonym: "RNA-seq evidence used in manual assertion" EXACT [] synonym: "RNAseq evidence used in manual assertion" EXACT [] synonym: "whole transcriptome shotgun sequencing" BROAD [] synonym: "WTSS evidence" BROAD [] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000295 ! RNA-sequencing evidence intersection_of: ECO:0000295 ! RNA-sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2017-06-28T10:37:02Z [Term] id: ECO:0006069 name: RNA-sequencing evidence used in automatic assertion def: "A type of high throughput nucleotide sequencing assay evidence used in an automatic assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] synonym: "RNA-seq evidence used in automatic assertion" EXACT [] synonym: "RNAseq evidence used in automatic assertion" EXACT [] synonym: "whole transcriptome shotgun sequencing" BROAD [] synonym: "WTSS evidence" BROAD [] is_a: ECO:0000295 ! RNA-sequencing evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000295 ! RNA-sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2017-06-28T10:37:02Z [Term] id: ECO:0006070 name: immuno-labelling electron microscopy evidence def: "A type of electron microscopy evidence resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] synonym: "immunolabelling electron microscopy evidence" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence property_value: IAO:0000234 "dosumis" xsd:string created_by: rctauber creation_date: 2017-07-05T09:59:10Z [Term] id: ECO:0006071 name: immuno-labelling electron microscopy evidence used in manual assertion def: "A type of electron microscopy evidence used in a manual assertion resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] synonym: "immunolabelling electron microscopy evidence" BROAD [] is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion is_a: ECO:0006070 ! immuno-labelling electron microscopy evidence intersection_of: ECO:0006070 ! immuno-labelling electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000119 PMID:25151300 xsd:string property_value: IAO:0000234 "dosumis" xsd:string created_by: rctauber creation_date: 2017-07-05T09:59:10Z [Term] id: ECO:0006072 name: immunofluorescence super resolution microscopy evidence def: "A type of super-resolution microscopy evidence resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] is_a: ECO:0005578 ! super-resolution microscopy evidence property_value: IAO:0000234 "dosumis" xsd:string created_by: rctauber creation_date: 2017-07-05T09:59:10Z [Term] id: ECO:0006073 name: immunofluorescence super resolution microscopy evidence used in manual assertion def: "A type of super-resolution microscopy evidence used in a manual assertion resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] is_a: ECO:0006004 {is_inferred="true"} ! super-resolution microscopy evidence used in manual assertion is_a: ECO:0006072 ! immunofluorescence super resolution microscopy evidence intersection_of: ECO:0006072 ! immunofluorescence super resolution microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "dosumis" xsd:string created_by: rctauber creation_date: 2017-07-05T09:59:10Z [Term] id: ECO:0006074 name: co-purification evidence used in manual assertion def: "A type of physical interaction evidence that is used in manual assertion where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] synonym: "co-purification" BROAD [] is_a: ECO:0000022 ! co-purification evidence is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion intersection_of: ECO:0000022 ! co-purification evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:19Z [Term] id: ECO:0006075 name: affinity evidence used in manual assertion def: "A type of physical interaction evidence that is used in manual assertion that depends on the strength of the interaction between two entities." [ECO:MCC] synonym: "ligand binding evidence" RELATED [] is_a: ECO:0000023 ! affinity evidence is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion intersection_of: ECO:0000023 ! affinity evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:20Z [Term] id: ECO:0006076 name: protein binding evidence used in manual assertion def: "A type of affinity evidence that is used in manual assertion resulting from the binding of a molecule to a protein or protein complex." [GO:0005515] is_a: ECO:0000024 ! protein binding evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0000024 ! protein binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:21Z [Term] id: ECO:0006077 name: bait-prey hybrid interaction evidence used in manual assertion def: "A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC] synonym: "bait-prey protein pull-down evidence" EXACT [] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence is_a: ECO:0006392 {is_inferred="true"} ! bait-prey interaction evidence used in manual assertion intersection_of: ECO:0000025 ! bait-prey hybrid interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:22Z [Term] id: ECO:0006078 name: immunological assay evidence used in manual assertion def: "A type of affinity evidence that is used in manual assertion resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362] is_a: ECO:0000040 ! immunological assay evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0000040 ! immunological assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:23Z [Term] id: ECO:0006079 name: yeast one-hybrid evidence used in manual assertion def: "A type of hybrid interaction evidence that is used in manual assertion that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC] synonym: "yeast one-hybrid assay" BROAD [] is_a: ECO:0000066 ! yeast one-hybrid evidence is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion intersection_of: ECO:0000066 ! yeast one-hybrid evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:24Z [Term] id: ECO:0006080 name: split-ubiquitin functional complementation evidence used in manual assertion def: "A type of split-ubiquitin functional complementation evidence that is used in a manual assertion that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] synonym: "split-ubiquitin assay" BROAD [] is_a: ECO:0000074 ! split-ubiquitin functional complementation evidence is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion intersection_of: ECO:0000074 ! split-ubiquitin functional complementation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:25Z [Term] id: ECO:0006081 name: far-Western blotting evidence used in manual assertion def: "A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] synonym: "far-Western analysis" BROAD [] is_a: ECO:0000076 ! far-Western blotting evidence is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion intersection_of: ECO:0000076 ! far-Western blotting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:26Z [Term] id: ECO:0006082 name: affinity chromatography evidence used in manual assertion def: "A type of affinity evidence that is used in a manual assertion that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." [ECO:MCC] synonym: "affinity chromatography" BROAD [] is_a: ECO:0000079 ! affinity chromatography evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0000079 ! affinity chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:27Z [Term] id: ECO:0006083 name: nucleic acid binding evidence used in manual assertion def: "A type of affinity evidence that is used in a manual assertion resulting from the binding of a molecule to a nucleic acid." [GO:0003676] is_a: ECO:0000136 ! nucleic acid binding evidence is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion intersection_of: ECO:0000136 ! nucleic acid binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:28Z [Term] id: ECO:0006084 name: ribohomopolymer binding assay evidence used in manual assertion def: "A type of nucleic acid binding evidence that is used in a manual assertion resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:PMC102612] synonym: "ribohomopolymer binding assay" BROAD [] is_a: ECO:0000138 ! ribohomopolymer binding assay evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0000138 ! ribohomopolymer binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:29Z [Term] id: ECO:0006085 name: protein:ion binding evidence used in manual assertion def: "A type of protein binding evidence that is used in a manual assertion resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] is_a: ECO:0000142 ! protein:ion binding evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion intersection_of: ECO:0000142 ! protein:ion binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:30Z [Term] id: ECO:0006086 name: Southwestern blot evidence used in manual assertion def: "A type of nucleic acid binding evidence that is used in a manual assertion in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] synonym: "Southwestern analysis" BROAD [] is_a: ECO:0000144 ! Southwestern blot evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0000144 ! Southwestern blot evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:31Z [Term] id: ECO:0006087 name: Northwestern blot evidence used in manual assertion def: "A type of nucleic acid binding evidence that is used in a manual assertion in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] synonym: "Northwestern analysis" BROAD [] is_a: ECO:0000146 ! Northwestern blot evidence is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0000146 ! Northwestern blot evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:32Z [Term] id: ECO:0006088 name: systematic evolution of ligands by exponential amplification evidence used in manual assertion def: "A type of evidence that is used in a manual assertion arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC] synonym: "in vitro evolution evidence" RELATED [] synonym: "in vitro selection evidence" BROAD [] synonym: "SELEX evidence" BROAD [] is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion intersection_of: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:33Z [Term] id: ECO:0006089 name: bacterial one-hybrid evidence used in manual assertion def: "A type of hybrid interaction evidence that is used in a manual assertion that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] synonym: "B1H evidence" BROAD [] is_a: ECO:0000294 ! bacterial one-hybrid evidence is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion intersection_of: ECO:0000294 ! bacterial one-hybrid evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:34Z [Term] id: ECO:0006090 name: protein-oligonucleotide microarray binding evidence used in manual assertion def: "A type of protein-oligonucleotide microarray binding evidence that is used in a manual assertion that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] synonym: "PBM evidence" BROAD [] is_a: ECO:0001601 ! protein-oligonucleotide microarray binding evidence is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion intersection_of: ECO:0001601 ! protein-oligonucleotide microarray binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:35Z [Term] id: ECO:0006091 name: functional complementation evidence used in manual assertion def: "A type of genetic interaction evidence that is used in a manual assertion where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] synonym: "functional complementation" BROAD [] is_a: ECO:0000012 ! functional complementation evidence is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion intersection_of: ECO:0000012 ! functional complementation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:36Z [Term] id: ECO:0006092 name: transgenic rescue experiment evidence used in manual assertion def: "A type of functional complementation evidence that is used in a manual assertion that is used in manual assertion resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] is_a: ECO:0000013 ! transgenic rescue experiment evidence is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion intersection_of: ECO:0000013 ! transgenic rescue experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:37Z [Term] id: ECO:0006093 name: transient rescue experiment evidence used in manual assertion def: "A type of transient rescue experiment evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000038 ! transient rescue experiment evidence is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion intersection_of: ECO:0000038 ! transient rescue experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:38Z [Term] id: ECO:0006094 name: suppressor/enhancer interaction phenotypic evidence used in manual assertion def: "A type of suppressor/enhancer interaction phenotypic evidence that is used in a manual assertion." [url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" BROAD [] synonym: "suppressor/enhancer interaction evidence used in manual assertion" EXACT [] is_a: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion intersection_of: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:39Z [Term] id: ECO:0006095 name: double mutant phenotypic evidence used in manual assertion def: "A type of double mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "double mutant analysis" BROAD [] synonym: "double mutant phenotype evidence used in manual assertion" EXACT [] is_a: ECO:0000054 ! double mutant phenotypic evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000054 ! double mutant phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:40Z [Term] id: ECO:0006096 name: epistatic interaction phenotypic evidence used in manual assertion def: "A type of epistatic interaction phenotypic evidence that is used in a manual assertion." [PMID:18852697] synonym: "epistatic interaction evidence used in manual assertion" EXACT [] synonym: "epistatic interactions" BROAD [] is_a: ECO:0000056 ! epistatic interaction phenotypic evidence is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion intersection_of: ECO:0000056 ! epistatic interaction phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:41Z [Term] id: ECO:0006097 name: functional complementation in heterologous system evidence used in manual assertion def: "A type of functional complementation evidence that is used in a manual assertion that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] synonym: "functional complementation in heterologous system" BROAD [] is_a: ECO:0000064 ! functional complementation in heterologous system evidence is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion intersection_of: ECO:0000064 ! functional complementation in heterologous system evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IGI" xsd:string created_by: rctauber creation_date: 2017-09-14T09:19:42Z [Term] id: ECO:0006098 name: temperature-sensitive mutant phenotypic evidence def: "A type of mutant phenotype evidence resulting from altered gene function at higher temperatures." [GOC:PG, PMID:19596904] synonym: "temperature-sensitive mutant phenotype evidence" EXACT [] synonym: "Ts mutation evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 "pgaudet" xsd:string created_by: rctauber creation_date: 2017-09-18T07:58:28Z [Term] id: ECO:0006099 name: temperature-sensitive mutant phenotypic evidence used in manual assertion def: "A type of temperature-sensitive mutant phenotypic evidence that is used in a manual assertion." [GOC:PG, PMID:19596904] synonym: "temperature-sensitive mutant phenotype evidence used in manual assertion" EXACT [] synonym: "Ts mutation evidence" BROAD [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence intersection_of: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 "pgaudet" xsd:string created_by: rctauber creation_date: 2017-09-18T07:58:28Z [Term] id: ECO:0006100 name: recessive mutant phenotype evidence def: "A type of mutant phenotype evidence based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] is_a: ECO:0000015 ! mutant phenotype evidence property_value: IAO:0000234 "pgaudet" xsd:string created_by: rctauber creation_date: 2017-09-18T07:58:28Z [Term] id: ECO:0006101 name: recessive mutant phenotype evidence used in manual assertion def: "A type of mutant phenotype evidence that is used in a manual assertion based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0006100 ! recessive mutant phenotype evidence intersection_of: ECO:0006100 ! recessive mutant phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string property_value: IAO:0000234 "pgaudet" xsd:string created_by: rctauber creation_date: 2017-09-18T07:58:28Z [Term] id: ECO:0006135 name: quantum mechanics/molecular mechanics simulation evidence def: "A type of computational evidence where the active site of the molecular system is described with highly accurate quantum theory, while the contribution of the rest of the system is described with molecular mechanical force field." [ECO:SN, PMID:26930454] synonym: "QM/MM evidence" EXACT [] is_a: ECO:0007672 ! computational evidence created_by: snadendla [Term] id: ECO:0006136 name: quantum mechanics/molecular mechanics simulation evidence used in automatic assertion def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006137 name: quantum mechanics/molecular mechanics simulation evidence used in manual assertion def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006138 name: molecular mechanics simulation evidence def: "A type of computational evidence where the energy of a molecular system is predicted as a function of its conformation." [ECO:SN, url:http\://vergil.chemistry.gatech.edu/courses/chem6485/pdf/molmech.pdf] synonym: "MM evidence" EXACT [] is_a: ECO:0007672 ! computational evidence created_by: snadendla [Term] id: ECO:0006139 name: molecular mechanics simulation evidence used in automatic assertion def: "A type of molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006138 ! molecular mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006140 name: molecular mechanics simulation evidence used in manual assertion def: "A type of molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] is_a: ECO:0006138 ! molecular mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006141 name: quantum mechanics simulation evidence def: "A type of computational evidence where the behavior of matter and light on the atomic and subatomic scale is described." [ECO:SN, url:https\://www.britannica.com/science/quantum-mechanics-physics] synonym: "QM evidence" EXACT [] synonym: "quantum physics evidence" EXACT [] synonym: "quantum theory evidence" EXACT [] is_a: ECO:0007672 ! computational evidence created_by: snadendla [Term] id: ECO:0006142 name: quantum mechanics simulation evidence used in automatic assertion def: "A type of quantum mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006141 ! quantum mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006143 name: quantum mechanics simulation evidence used in manual assertion def: "A type of quantum mechanics simulation evidence that is used in manual assertion." [ECO:SN] is_a: ECO:0006141 ! quantum mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006144 name: density functional theory simulation evidence def: "A type of quantum mechanics simulation evidence that results from the calculation of ground-state electronic structure of atoms, molecules and solid state materials." [ECO:SN, url:https\://www.sciencedirect.com/topics/physics-and-astronomy/density-functional-theory] synonym: "DFT evidence" EXACT [] is_a: ECO:0006141 ! quantum mechanics simulation evidence created_by: snadendla [Term] id: ECO:0006145 name: density functional theory simulation evidence used in manual assertion def: "A type of density functional theory simulation evidence that is used in manual assertion." [ECO:SN] is_a: ECO:0006144 ! density functional theory simulation evidence created_by: snadendla [Term] id: ECO:0006146 name: density functional theory simulation evidence used in automatic assertion def: "A type of density functional theory simulation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006144 ! density functional theory simulation evidence created_by: snadendla [Term] id: ECO:0006151 name: documented statement evidence def: "A type of evidence in which information is recorded in some documentation system, for example, but not limited to a publication, survey, or medical record." [ECO:SN] is_a: ECO:0000000 ! evidence created_by: snadendla [Term] id: ECO:0006152 name: medical practitioner statement evidence def: "A type of documented statement evidence in which information is stated by a health care professional, for example, but not limited to a doctor, nurse, or psychologist." [ECO:SN] is_a: ECO:0006151 ! documented statement evidence created_by: snadendla [Term] id: ECO:0006153 name: self-reported individual's statement evidence def: "A type of documented statement evidence in which information is provided by an individual through means including, but not limited to, paper form, electronic form, or verbal communication, and is captured in a documented record." [ECO:SN] is_a: ECO:0006151 ! documented statement evidence created_by: snadendla [Term] id: ECO:0006154 name: self-reported patient statement evidence def: "A type of self-reported individual's statement evidence in which information is provided by a patient in a clinical setting." [ECO:SN] is_a: ECO:0006153 ! self-reported individual's statement evidence property_value: IAO:0000234 "Pablo Botas for Foundation 29, Dx29 project" xsd:string created_by: snadendla [Term] id: ECO:0006155 name: documented statement evidence used in manual assertion def: "A type of documented statement evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion is_a: ECO:0006151 ! documented statement evidence intersection_of: ECO:0006151 ! documented statement evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: snadendla [Term] id: ECO:0006156 name: documented statement evidence used in automatic assertion def: "A type of documented statement evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion is_a: ECO:0006151 ! documented statement evidence intersection_of: ECO:0006151 ! documented statement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla [Term] id: ECO:0006157 name: self-reported individual's statement evidence used in manual assertion def: "A type of self-reported individual's statement evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006153 ! self-reported individual's statement evidence is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion intersection_of: ECO:0006153 ! self-reported individual's statement evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: snadendla [Term] id: ECO:0006158 name: self-reported individual's statement evidence used in automatic assertion def: "A type of self-reported individual's statement evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006153 ! self-reported individual's statement evidence is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion intersection_of: ECO:0006153 ! self-reported individual's statement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla [Term] id: ECO:0006159 name: self-reported patient statement evidence used in manual assertion def: "A type of self-reported patient statement evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006154 ! self-reported patient statement evidence is_a: ECO:0006157 {is_inferred="true"} ! self-reported individual's statement evidence used in manual assertion intersection_of: ECO:0006154 ! self-reported patient statement evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: snadendla [Term] id: ECO:0006160 name: self-reported patient statement evidence used in automatic assertion def: "A type of self-reported patient statement evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006154 ! self-reported patient statement evidence is_a: ECO:0006158 {is_inferred="true"} ! self-reported individual's statement evidence used in automatic assertion intersection_of: ECO:0006154 ! self-reported patient statement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla [Term] id: ECO:0006161 name: medical practitioner statement evidence used in manual assertion def: "A type of medical practitioner statement evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006152 ! medical practitioner statement evidence is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion intersection_of: ECO:0006152 ! medical practitioner statement evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: snadendla [Term] id: ECO:0006162 name: medical practitioner statement evidence used in automatic assertion def: "A type of medical practitioner statement evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006152 ! medical practitioner statement evidence is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion intersection_of: ECO:0006152 ! medical practitioner statement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla [Term] id: ECO:0006163 name: nuclear magnetic resonance spectroscopy evidence def: "A type of nuclear magnetic resonance evidence used for quantification of metabolites or for the determination of chemical structure or composition." [ECO:SN, PMID:16428685, PMID:23036848] synonym: "NMR Spectroscopy" EXACT [] is_a: ECO:0001105 ! nuclear magnetic resonance evidence relationship: OBI:0000312 OBI:0000623 property_value: IAO:0000234 SIB:PG xsd:string created_by: snadendla [Term] id: ECO:0006164 name: nuclear magnetic resonance spectroscopy evidence used in automatic assertion def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence is_a: ECO:0007441 {is_inferred="true"} ! nuclear magnetic resonance evidence used in automatic assertion intersection_of: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: snadendla [Term] id: ECO:0006165 name: nuclear magnetic resonance spectroscopy evidence used in manual assertion def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "NMR spectroscopy evidence" BROAD [] is_a: ECO:0001238 {is_inferred="true"} ! nuclear magnetic resonance evidence used in manual assertion is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence intersection_of: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 SIB:PG xsd:string created_by: snadendla [Term] id: ECO:0006166 name: nuclear magnetic resonance imaging evidence def: "A type of nuclear magnetic resonance evidence used to image anatomy and physiological processes." [url:https\://en.wikipedia.org/wiki/Magnetic_resonance_imaging] is_a: ECO:0001105 ! nuclear magnetic resonance evidence property_value: IAO:0000234 "bjonnh" xsd:string created_by: snadendla [Term] id: ECO:0006167 name: nuclear magnetic resonance imaging evidence used in automatic assertion def: "A type of nuclear magnetic resonance imaging evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence is_a: ECO:0007441 {is_inferred="true"} ! nuclear magnetic resonance evidence used in automatic assertion intersection_of: ECO:0006166 ! nuclear magnetic resonance imaging evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "bjonnh" xsd:string created_by: snadendla [Term] id: ECO:0006168 name: nuclear magnetic resonance imaging evidence used in manual assertion def: "A type of nuclear magnetic resonance imaging evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001238 {is_inferred="true"} ! nuclear magnetic resonance evidence used in manual assertion is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence intersection_of: ECO:0006166 ! nuclear magnetic resonance imaging evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "bjonnh" xsd:string created_by: snadendla [Term] id: ECO:0006169 name: quantitative western immunoblotting evidence def: "A type of qualitative western immunoblotting evidence where detection of the signal is carried out through digital imaging and the signal is normalized with methods such as standard curves built from reference housekeeping proteins or total transferred protein measurements." [ECO:SN, PMID:25852189] is_a: ECO:0000112 ! qualitative western immunoblotting evidence property_value: IAO:0000234 GO:Val xsd:string created_by: snadendla [Term] id: ECO:0006170 name: quantitative western immunoblotting evidence used in manual assertion def: "A type of quantitative western immunoblotting evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000279 {is_inferred="true"} ! qualitative western immunoblotting evidence used in manual assertion is_a: ECO:0006169 ! quantitative western immunoblotting evidence intersection_of: ECO:0006169 ! quantitative western immunoblotting evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: snadendla [Term] id: ECO:0006171 name: quantitative western immunoblotting evidence used in automatic assertion def: "A type of quantitatative western immunoblotting evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006169 ! quantitative western immunoblotting evidence is_a: ECO:0007306 {is_inferred="true"} ! qualitative western immunoblotting evidence used in automatic assertion intersection_of: ECO:0006169 ! quantitative western immunoblotting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: snadendla [Term] id: ECO:0006172 name: mixed support of intron positions by RNA-sequencing alignment evidence def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignments from a mixture of samples support all of the intron positions (exon pairs) predicted for a transcript." [ECO:SN] comment: Mixed support entails that all exon pairs represented in the transcript are supported, but require a combination of evidence from multiple samples. For example, for a transcript containing five exons, if liver RNA-seq reads support the first two introns and brain RNA-seq supports the last three introns, then neither sample meets the criteria for full support (ECO:0000343) but the combination of samples qualifies as Mixed support. Mixed support may result from differential expression, or low expression levels that benefit from combining data. synonym: "mixed support of intron positions by RNA-seq alignment evidence" EXACT [] is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence property_value: IAO:0000234 "NCBI-RefSeq" xsd:string created_by: snadendla [Term] id: ECO:0006173 name: mixed support of intron positions by RNA-sequencing alignment evidence used in automatic assertion def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:SN] synonym: "mixed support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence intersection_of: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 "NCBI-RefSeq" xsd:string created_by: snadendla [Term] id: ECO:0006174 name: mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion" [ECO:SN] synonym: "mixed support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence intersection_of: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string property_value: IAO:0000234 "NCBI-RefSeq" xsd:string created_by: snadendla [Term] id: ECO:0006175 name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence def: "A type of nuclear magnetic resonance spectroscopy evidence resulting from measuring the change in a certain type of NMR spectrum as a result of the spontenaous exchange of hydrogen and deuterium nuclei between the sample and the solvent." [PMID:20960033, PMID:28538145, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#NMR_spectroscopy] synonym: "NMR HDX" EXACT [] is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006176 name: proton-based nuclear magnetic resonance evidence def: "A type of nuclear magnetic resonance spectroscopy evidence in which the electromagnetic singal being measured comes from the perturbation of hydrogen nuclei resulting in a one dimensional spectrum." [url:https\://en.wikipedia.org/wiki/Proton_nuclear_magnetic_resonance] synonym: "1D NMR" BROAD [] synonym: "proton NMR" EXACT [] is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006177 name: circular dichroism evidence def: "A type of spectrometry evidence derived from irradiating a sample with light (either visible, infrared or ultraviolet wavelengths) and observing the difference of absorption of left-handed and right-handed light by the sample by comparing the extent of circular polarization in the incident and the emitted light." [PMID:17464384, url:https\://en.wikipedia.org/wiki/Circular_dichroism] synonym: "CD" EXACT [] is_a: ECO:0005031 ! structure determination evidence is_a: ECO:0005504 ! spectrometry evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006178 name: synchrotron radiation circular dichroism evidence def: "A type of circular dichroism evidence where the source of radiation is radially accelerated particles, most often moving on a circular path in a synchrotron, typically providing lower wavelengths and therefore higher signal-to-noise ratio compared to regular circular dichroism measurements." [PMID:20658968] synonym: "SRCD" EXACT [] is_a: ECO:0006177 ! circular dichroism evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006179 name: far-UV circular dichroism evidence def: "A type of circular dichroism evidence whose spectrum is in the range of 190-230 nm, providing information mainly about amide bonds and through which the relative proportion of secondary structure elements of a protein can be estimated." [PMID:17464384] synonym: "far-UV CD" EXACT [] is_a: ECO:0006177 ! circular dichroism evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006180 name: near-UV circular dichroism evidence def: "A type of circular dichroism evidence whose spectrum is in the range of 250-350 nm , providing information mainly about aromatic residues, i.e. phenylalanine, tyrosine and tryptophan, through which it provides information on the tertiary structure of a protein." [PMID:17464384] synonym: "near-UV CD" EXACT [] is_a: ECO:0006177 ! circular dichroism evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006181 name: cryogenic electron microscopy evidence def: "A type of electron microscopy evidence where the sample being studied has been cooled to cryogenic temperatures (typically below -153 degrees Celsius) prior to the experiment." [PMID:31078399, url:https\://en.wikipedia.org/wiki/Cryogenic_electron_microscopy] synonym: "cryo-EM" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006182 name: small-angle X-ray scattering evidence def: "A type of light scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with an X-ray beam and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:26320411, url:https\://en.wikipedia.org/wiki/Small-angle_X-ray_scattering] synonym: "SAXS" EXACT [] is_a: ECO:0007061 ! light scattering evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006183 name: particle scattering evidence def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by irradiating the sample with a particle beam and the scattered intensity is probed at a certain angle by a detector." [DisProt:BalintMeszaros] is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006184 name: small-angle neutron scattering evidence def: "A type of particle scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with a beam of thermal neutrons and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:17714935, url:https\://en.wikipedia.org/wiki/Small-angle_neutron_scattering] synonym: "SANS" EXACT [] is_a: ECO:0006183 ! particle scattering evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006185 name: author inference def: "A type of inferential evidence where an assertion is derived by the authors of a scientific publication from another assertion and/or primary data via logical inference or other rational means." [DisProt:BalintMeszaros] is_a: ECO:0000204 ! author statement is_a: ECO:0000361 ! inferential evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006186 name: combinatorial experimental and author inference evidence contained in single publication def: "A type of combinatorial experimental and author inference evidence where the experimental results and author inference are contained in a single publication. " [ECO:MG] is_a: ECO:0007011 ! combinatorial experimental and author inference evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006187 name: X-ray crystallography-based structural model with missing residue coordinates def: "A type of X-ray cystallography evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] is_a: ECO:0001823 ! x-ray crystallography evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006188 name: X-ray crystallography-based structural model with high relative B-factor values def: "A type of X-ray cystallography evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] is_a: ECO:0001823 ! x-ray crystallography evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006189 name: cryogenic electron microscopy-based structural model with missing residue coordinates def: "A type of cryogenic electron microscopy evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] is_a: ECO:0006181 ! cryogenic electron microscopy evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006190 name: cryogenic electron microscopy-based structural model with high relative B-factor values def: "A type of cryogenic electron microscopy evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] is_a: ECO:0006181 ! cryogenic electron microscopy evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006191 name: Fourier-transform infrared spectroscopy evidence def: "A type of spectrophotometry evidence resulting from the evaluation of a molecule in a fluid or solid state by its ability to alter the transmission of infrared light, where the raw data collected is transformed into the specturm by Fourier transform." [url:https\://en.wikipedia.org/wiki/Fourier-transform_infrared_spectroscopy] synonym: "FT-IR" EXACT [] synonym: "FTIR" EXACT [] is_a: ECO:0001126 ! spectrophotometry evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006192 name: heat capacity-based evidence def: "A type of direct assay evidence derived from measuring the amount of heat absorbed or released by a sample to monitor chemical reactions, phase transitions or other processes affecting the physical state or chemical composition." [url:https\://en.wikipedia.org/wiki/Calorimetry] synonym: "calorimetry evidence" BROAD [] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006193 name: differential scanning calorimetry evidence def: "A type of heat capacity-based evidence where the heat required to increase the temperature of the sample is continuously monitored and compared to the heat required to increase the temperature of a reference (having a largely identical composition compared to the sample, such as the empty buffer compared to a protein solution) over a range of temperatures." [url:https\://en.wikipedia.org/wiki/Differential_scanning_calorimetry] synonym: "DSC" EXACT [] is_a: ECO:0006192 ! heat capacity-based evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006194 name: selective antibody-based structural conformation evidence def: "A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule." [DisProt:BalintMeszaros] is_a: ECO:0005031 ! structure determination evidence is_a: ECO:0005593 ! immunodetection assay evidence property_value: IAO:0000112 "Here, we describe the generation, characterisation, and utility of a monoclonal antibody that selectively binds with high affinity to the asymmetric TNF trimer–small molecule complex. The antibody helps to define the molecular dynamics of the apo TNF trimer, reveals the mode of action and specificity of the small molecule inhibitors, acts as a chaperone in solving the human TNF–TNFR1 complex crystal structure, and facilitates the measurement of small molecule target occupancy in complex biological samples." xsd:string {xref="PMID:33495445"} property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006195 name: protein hydrogen-deuterium exchange mass spectrometry evidence def: "A type of mass spectrometry evidence derived from measuring changes in mass associated with the isotopic exchange between amide hydrogens of the protein backbone and its surrounding solvent, providing information on the structural state, the dynamics and the intrinsic chemical properties of the underlying amino acid sequence." [PMID:31249422, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#Mass_spectrometry] synonym: "HDX-MS" EXACT [] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006196 name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in a manual assertion." [ECO:SN] synonym: "NMR HDX" EXACT [] is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence intersection_of: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006197 name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in automatic assertion def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in an automatic assertion." [ECO:SN] synonym: "NMR HDX" EXACT [] is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence intersection_of: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006198 name: proton-based nuclear magnetic resonance evidence used in manual assertion def: "A type of proton-based nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:SN] synonym: "1D NMR" BROAD [] synonym: "proton NMR" EXACT [] is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence intersection_of: ECO:0006176 ! proton-based nuclear magnetic resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006199 name: proton-based nuclear magnetic resonance evidence used in automatic assertion def: "A type of proton-based nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] synonym: "1D NMR" BROAD [] synonym: "proton NMR" EXACT [] is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence intersection_of: ECO:0006176 ! proton-based nuclear magnetic resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006200 name: circular dichroism evidence used in manual assertion def: "A type of circular dichroism evidence that is used in a manual assertion." [ECO:SN] synonym: "CD" EXACT [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006177 ! circular dichroism evidence intersection_of: ECO:0006177 ! circular dichroism evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006201 name: circular dichroism evidence used in automatic assertion def: "A type of circular dichroism evidence that is used in an automatic assertion." [ECO:SN] synonym: "CD" EXACT [] is_a: ECO:0006177 ! circular dichroism evidence is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion intersection_of: ECO:0006177 ! circular dichroism evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006202 name: synchrotron radiation circular dichroism evidence used in manual assertion def: "A type of synchrotron radiation circular dichroism evidence that is used in a manual assertion." [ECO:SN] synonym: "SRCD" EXACT [] is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion intersection_of: ECO:0006178 ! synchrotron radiation circular dichroism evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006203 name: synchrotron radiation circular dichroism evidence used in automatic assertion def: "A type of synchrotron radiation circular dichroism evidence that is used in an automatic assertion." [ECO:SN] synonym: "SRCD" EXACT [] is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion intersection_of: ECO:0006178 ! synchrotron radiation circular dichroism evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006204 name: far-UV circular dichroism evidence used in manual assertion def: "A type of far-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] synonym: "far-UV CD" EXACT [] is_a: ECO:0006179 ! far-UV circular dichroism evidence is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion intersection_of: ECO:0006179 ! far-UV circular dichroism evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006205 name: far-UV circular dichroism evidence used in automatic assertion def: "A type of far-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] synonym: "far-UV CD" EXACT [] is_a: ECO:0006179 ! far-UV circular dichroism evidence is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion intersection_of: ECO:0006179 ! far-UV circular dichroism evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006206 name: near-UV circular dichroism evidence used in manual assertion def: "A type of near-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] synonym: "near-UV CD" EXACT [] is_a: ECO:0006180 ! near-UV circular dichroism evidence is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion intersection_of: ECO:0006180 ! near-UV circular dichroism evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006207 name: near-UV circular dichroism evidence used in automatic assertion def: "A type of near-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] synonym: "near-UV CD" EXACT [] is_a: ECO:0006180 ! near-UV circular dichroism evidence is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion intersection_of: ECO:0006180 ! near-UV circular dichroism evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006208 name: cryogenic electron microscopy evidence used in manual assertion def: "A type of cryogenic electron microscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "cryo-EM" EXACT [] is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion is_a: ECO:0006181 ! cryogenic electron microscopy evidence intersection_of: ECO:0006181 ! cryogenic electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006209 name: cryogenic electron microscopy evidence used in automatic assertion def: "A type of cryogenic electron microscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "cryo-EM" EXACT [] is_a: ECO:0006181 ! cryogenic electron microscopy evidence is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion intersection_of: ECO:0006181 ! cryogenic electron microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006210 name: small-angle X-ray scattering evidence used in manual assertion def: "A type of small-angle X-ray scattering evidence that is used in a manual assertion." [ECO:SN] synonym: "SAXS" EXACT [] is_a: ECO:0006182 ! small-angle X-ray scattering evidence is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion intersection_of: ECO:0006182 ! small-angle X-ray scattering evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006211 name: small-angle X-ray scattering evidence used in automatic assertion def: "A type of small-angle X-ray scattering evidence that is used in an automatic assertion." [ECO:SN] synonym: "SAXS" EXACT [] is_a: ECO:0006182 ! small-angle X-ray scattering evidence is_a: ECO:0007617 {is_inferred="true"} ! light scattering evidence used in automatic assertion intersection_of: ECO:0006182 ! small-angle X-ray scattering evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006212 name: particle scattering evidence used in manual assertion def: "A type of particle scattering evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006183 ! particle scattering evidence intersection_of: ECO:0006183 ! particle scattering evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006213 name: particle scattering evidence used in automatic assertion def: "A type of particle scattering evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006183 ! particle scattering evidence is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0006183 ! particle scattering evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006214 name: small-angle neutron scattering evidence used in manual assertion def: "A type of small-angle neutron scattering evidence that is used in a manual assertion." [ECO:SN] synonym: "SANS" EXACT [] is_a: ECO:0006184 ! small-angle neutron scattering evidence is_a: ECO:0006212 {is_inferred="true"} ! particle scattering evidence used in manual assertion intersection_of: ECO:0006184 ! small-angle neutron scattering evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006215 name: small-angle neutron scattering evidence used in automatic assertion def: "A type of small-angle neutron scattering evidence that is used in an automatic assertion." [ECO:SN] synonym: "SANS" EXACT [] is_a: ECO:0006184 ! small-angle neutron scattering evidence is_a: ECO:0006213 {is_inferred="true"} ! particle scattering evidence used in automatic assertion intersection_of: ECO:0006184 ! small-angle neutron scattering evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006216 name: author inference used in manual assertion def: "A type of author inference that is used in a manual assertion." [ECO:SN] is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion is_a: ECO:0006185 ! author inference is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion intersection_of: ECO:0006185 ! author inference intersection_of: used_in ECO:0000218 ! manual assertion property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006217 name: author inference used in automatic assertion def: "A type of author inference that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006185 ! author inference is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion intersection_of: ECO:0006185 ! author inference intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006218 name: combinatorial experimental and author inference evidence contained in single publication used in manual assertion def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in a manual assertion." [ECO:SN] is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication is_a: ECO:0007013 {is_inferred="true"} ! combinatorial experimental and author inference evidence used in manual assertion intersection_of: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication intersection_of: used_in ECO:0000218 ! manual assertion property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006219 name: combinatorial experimental and author inference evidence contained in single publication used in automatic assertion def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication is_a: ECO:0007592 {is_inferred="true"} ! combinatorial experimental and author inference evidence used in automatic assertion intersection_of: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006220 name: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] is_a: ECO:0005670 {is_inferred="true"} ! x-ray crystallography evidence used in manual assertion is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates intersection_of: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006221 name: X-ray crystallography-based structural model with missing residue coordinates used in automatic assertion def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005671 {is_inferred="true"} ! x-ray crystallography evidence used in automatic assertion is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates intersection_of: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006222 name: X-ray crystallography-based structural model with high relative B-factor values used in manual assertion def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] is_a: ECO:0005670 {is_inferred="true"} ! x-ray crystallography evidence used in manual assertion is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values intersection_of: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006223 name: X-ray crystallography-based structural model with high relative B-factor values used in automatic assertion def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] is_a: ECO:0005671 {is_inferred="true"} ! x-ray crystallography evidence used in automatic assertion is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values intersection_of: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006224 name: cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates is_a: ECO:0006208 {is_inferred="true"} ! cryogenic electron microscopy evidence used in manual assertion intersection_of: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006225 name: cryogenic electron microscopy-based structural model with missing residue coordinates used in automatic assertion def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates is_a: ECO:0006209 {is_inferred="true"} ! cryogenic electron microscopy evidence used in automatic assertion intersection_of: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006226 name: cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values is_a: ECO:0006208 {is_inferred="true"} ! cryogenic electron microscopy evidence used in manual assertion intersection_of: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006227 name: cryogenic electron microscopy-based structural model with high relative B-factor values used in automatic assertion def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values is_a: ECO:0006209 {is_inferred="true"} ! cryogenic electron microscopy evidence used in automatic assertion intersection_of: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006228 name: Fourier-transform infrared spectroscopy evidence used in manual assertion def: "A type of Fourier-transform infrared spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "FT-IR" EXACT [] synonym: "FTIR" EXACT [] is_a: ECO:0001258 {is_inferred="true"} ! spectrophotometry evidence used in manual assertion is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence intersection_of: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006229 name: Fourier-transform infrared spectroscopy evidence used in automatic assertion def: "A type of Fourier-transform infrared spectroscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "FT-IR" EXACT [] synonym: "FTIR" EXACT [] is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence is_a: ECO:0007458 {is_inferred="true"} ! spectrophotometry evidence used in automatic assertion intersection_of: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006230 name: heat capacity-based evidence used in manual assertion def: "A type of heat capacity-based evidence that is used in a manual assertion." [ECO:SN] synonym: "calorimetry evidence" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006192 ! heat capacity-based evidence intersection_of: ECO:0006192 ! heat capacity-based evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006231 name: heat capacity-based evidence used in automatic assertion def: "A type of heat capacity-based evidence that is used in an automatic assertion." [ECO:SN] synonym: "calorimetry evidence" BROAD [] is_a: ECO:0006192 ! heat capacity-based evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0006192 ! heat capacity-based evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006232 name: differential scanning calorimetry evidence used in manual assertion def: "A type of differential scanning calorimetry evidence that is used in a manual assertion." [ECO:SN] synonym: "DSC" EXACT [] is_a: ECO:0006193 ! differential scanning calorimetry evidence is_a: ECO:0006230 {is_inferred="true"} ! heat capacity-based evidence used in manual assertion intersection_of: ECO:0006193 ! differential scanning calorimetry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006233 name: differential scanning calorimetry evidence used in automatic assertion def: "A type of differential scanning calorimetry evidence that is used in an automatic assertion." [ECO:SN] synonym: "DSC" EXACT [] is_a: ECO:0006193 ! differential scanning calorimetry evidence is_a: ECO:0006231 {is_inferred="true"} ! heat capacity-based evidence used in automatic assertion intersection_of: ECO:0006193 ! differential scanning calorimetry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006234 name: selective antibody-based structural conformation evidence used in manual assertion def: "A type of selective antibody-based structural conformation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006194 ! selective antibody-based structural conformation evidence intersection_of: ECO:0006194 ! selective antibody-based structural conformation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006235 name: selective antibody-based structural conformation evidence used in automatic assertion def: "A type of selective antibody-based structural conformation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006194 ! selective antibody-based structural conformation evidence is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0006194 ! selective antibody-based structural conformation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006236 name: protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in a manual assertion." [ECO:SN] synonym: "HDX-MS" EXACT [] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence intersection_of: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006237 name: protein hydrogen-deuterium exchange mass spectrometry evidence used in automatic assertion def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] synonym: "HDX-MS" EXACT [] is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006238 name: galactokinase reporter gene assay evidence def: "A type of reporter gene assay evidence based on the fusion of the galK gene to a specific promoter for the expression of the enzyme galactokinase." [url:https\://www.sciencedirect.com/science/article/pii/007668796608039X] is_a: ECO:0000049 ! reporter gene assay evidence property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006239 name: polyadenylated transcript 3'-end-sequencing evidence def: "A type of RNA-sequencing evidence based on sequencing of the 3' ends of cDNA molecules that are selected by presence of a polyadenylated tail." [PMID:31617559] is_a: ECO:0000295 ! RNA-sequencing evidence property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string created_by: snadendla [Term] id: ECO:0006240 name: colony boundary assay evidence def: "A type of colony morphology phenotypic evidence based on the presence or absence of a visually apparent boundary formed between colonies of multiple isolates or strains of the same microbial species growing on a solid surface such as that of an agar culture medium." [PMID:18621670] is_a: ECO:0007077 ! colony morphology phenotypic evidence property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006241 name: galactokinase reporter gene assay evidence used in manual assertion def: "A type of galactokinase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0006238 ! galactokinase reporter gene assay evidence intersection_of: ECO:0006238 ! galactokinase reporter gene assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006242 name: galactokinase reporter gene assay evidence used in automatic assertion def: "A type of galactokinase reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006238 ! galactokinase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0006238 ! galactokinase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006243 name: polyadenylated transcript 3'-end-sequencing evidence used in manual assertion def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence intersection_of: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string created_by: snadendla [Term] id: ECO:0006244 name: polyadenylated transcript 3'-end-sequencing evidence used in automatic assertion def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence intersection_of: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string created_by: snadendla [Term] id: ECO:0006245 name: colony boundary assay evidence used in manual assertion def: "A type of colony boundary assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006240 ! colony boundary assay evidence is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion intersection_of: ECO:0006240 ! colony boundary assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006246 name: colony boundary assay evidence used in automatic assertion def: "A type of colony boundary assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006240 ! colony boundary assay evidence is_a: ECO:0007625 {is_inferred="true"} ! colony morphology phenotypic evidence used in automatic assertion intersection_of: ECO:0006240 ! colony boundary assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 OMP:DebbySiegele xsd:string created_by: snadendla [Term] id: ECO:0006247 name: analytical ultracentrifugation evidence def: "A type of molecule detection assay evidence where the hydrodynamic behaviour of macromolecules is used to study their size, shape and interactions, by spinning the sample solution at high centrifugal field (up to above 100000 rpm and 1000000 g), and monitoring the evolution of sample concentration profile versus the axis of rotation." [PMID:12192063] synonym: "AUC" EXACT [] is_a: ECO:0005516 ! molecule detection assay evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006248 name: fluorescence polarization evidence def: "A type of physical interaction evidence based on the measurement of the change in the degree of polarization of a fluorophore upon binding to a molecular partner." [PMID:21372817] synonym: "FP" EXACT [] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006249 name: bait-prey protein pull-down evidence def: "A type of bait-prey interaction evidence resulting from an affinity purification in-vitro technique used to detect physical interactions between two or more proteins, where a molecule of interest - a \"bait\" - is immobilized and incubated with a protein source containing putative \"prey\" proteins." [PMID:28667618] is_a: ECO:0006351 ! bait-prey interaction evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006250 name: rotary shadowing electron microscopy evidence def: "A type of electron microscopy evidence where a heavy metal is evaporated onto surface adsorbed molecules and macromolecular assemblies allowing high-contrast visualization of both individual macromolecules and the surface structure of macromolecular assemblies." [PMID:19247619] synonym: "RS-TEM" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006251 name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence def: "A type of mass spectrometry evidence resulting from the ionization of polar functional groups producing multiple charged ions followed by the detection of ion cyclotron frequencies within a magnetic field, making it suitable for the study of large molecules (>2 kDa)." [PMID:10575730] synonym: "ESI FT-ICR MS" EXACT [] is_a: ECO:0001096 ! mass spectrometry evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006252 name: magnetic resonance evidence def: "A type of experimental evidence in which particles in strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting eletromagnetic signal is captured." [ECO:MG] is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006253 name: electron paramagnetic resonance evidence def: "A type of magnetic resonance evidence in which unpaired electrons in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Electron_paramagnetic_resonance] synonym: "EPR" EXACT [] synonym: "ESR" EXACT [] is_a: ECO:0006252 ! magnetic resonance evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006254 name: site-directed spin-labelling electron paramagnetic resonance evidence def: "A type of electron paramagnetic resonance evidence where the signal is obtained by labeling the protein in specific sites with a functional group (most often a nitroxide) containing an unpaired electron using site-directed mutagenesis." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Site-directed_spin_labeling] synonym: "SDSL" EXACT [] is_a: ECO:0006253 ! electron paramagnetic resonance evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006255 name: deglycosylation assay evidence def: "A type of direct assay evidence where the removal of glycan groups from a substrate (RNA, DNA, or protein) is detected." [url:https\://en.wikipedia.org/wiki/Glycosylation] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006256 name: protein fragment functional complementation evidence def: "A type of physical interaction evidence that is based on the detection of a protein-protein interaction between a bait and prey protein by reconstitution of a reporter protein serving as a detectable entity (usually an enzyme or a fluorescent protein), the two halves of which are each attached separately to the bait and prey." [PMID:31274323] is_a: ECO:0000021 ! physical interaction evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006257 name: beta galactosidase functional complementation evidence def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a functional beta galactosidase enzyme." [PMID:9237989] is_a: ECO:0006256 ! protein fragment functional complementation evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006258 name: GAL4-VP16 functional complementation evidence def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a transcriptional activation complex consisting of the GAL4 DNA-binding domain and the transactivation domain from the herpes simplex virus protein VP16." [MI:0728] is_a: ECO:0006256 ! protein fragment functional complementation evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006259 name: Raman spectroscopy evidence def: "A type of spectrophotometry evidence used to determine a structural fingerprint of the molecule in solution by probing its vibrational modes via the inelastic scattering of photons (called Raman scattering)." [url:https\://en.wikipedia.org/wiki/Raman_spectroscopy] synonym: "Raman optical activity" EXACT [] synonym: "ROA" EXACT [] is_a: ECO:0001126 ! spectrophotometry evidence is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006260 name: protein thermal shift assay evidence def: "A type of temperature-induced protein unfolding evidence that assess thermal denaturation temperature and protein stability under different experimental conditions, e.g. buffer pH or ionic strength, redox potential, usually by means of intrinsic fluorescence or thermofluor." [url:https\://en.wikipedia.org/wiki/Thermal_shift_assay] synonym: "TSA" EXACT [] is_a: ECO:0006268 ! temperature-induced protein unfolding evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006261 name: microscale thermophoresis evidence def: "A type of physical interaction evidence resulting from measuring the change of the fluorescent activity of a molecule as a function of binding to a non-fluorescent partner and its movement in a microspcopic thermal gradient affecting the intensity of fluorescence." [PMID:23270813, url:https\://en.wikipedia.org/wiki/Microscale_thermophoresis] synonym: "MST" EXACT [] is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006262 name: native protein gel electrophoresis evidence def: "A type of gel electrophoresis evidence resulting from the use of non-denaturing conditions so that the proteins being measured are in their native structural states." [url:https\://en.wikipedia.org/wiki/Gel_electrophoresis_of_proteins] synonym: "BN-PAGE" NARROW [] synonym: "CN-PAGE" NARROW [] synonym: "native gel" EXACT [] synonym: "native PAGE" NARROW [] synonym: "QPNC-PAGE" NARROW [] is_a: ECO:0000337 ! gel electrophoresis evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006263 name: turbidity measurement evidence def: "A type of direct assay evidence where the optical properties of a solution is measured in the visible light spectrum, assessing the haziness or cloudiness as a result of the properties of the solutes or the changes in their material states." [url:https\://en.wikipedia.org/wiki/Turbidity] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006264 name: competitive binding evidence def: "A type of affinity evidence derived from an experimental setup where the binding properties of a ligand are assessed by measuring the competition with another ligand with already known binding properties." [PMID:21115850] synonym: "competition assay" EXACT [] is_a: ECO:0000023 ! affinity evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006265 name: protein unfolding evidence def: "A type of structure determination evidence obtained by monitoring structural changes of a protein as a response to changes of an environmental parameter, such as pH or temperature, or the concentration of a denaturant, such as urea." [url:https\://en.wikipedia.org/wiki/Equilibrium_unfolding] synonym: "protein denaturation assay" EXACT [] is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006266 name: urea-induced protein unfolding evidence def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by increasing concentrations of urea." [PMID:19708649] synonym: "chemical denaturation" BROAD [] synonym: "chemical unfolding" BROAD [] is_a: ECO:0006265 ! protein unfolding evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006267 name: pH-induced protein unfolding evidence def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in pH." [PMID:23185611] is_a: ECO:0006265 ! protein unfolding evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006268 name: temperature-induced protein unfolding evidence def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in temperature." [PMID:23185611] synonym: "thermal dentauration" EXACT [] synonym: "thermal unfolding" EXACT [] is_a: ECO:0006265 ! protein unfolding evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006269 name: cell aggregation evidence def: "A type of cell-based assay evidence resulting from the analysis of cells irreversibly binding to each other." [PMID:24092433] is_a: ECO:0001565 ! cell-based assay evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006270 name: RNA-protein binding evidence def: "A type of affinity evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to RNA." [PMID:30804549] is_a: ECO:0000024 ! protein binding evidence is_a: ECO:0000136 ! nucleic acid binding evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006271 name: fluorescence microscopy evidence def: "A type of microscopy evidence that is obtained by using the fluorescence of the studied sample instead of or in addition to the scattering, reflection, attenuation or absorption of light." [url:https\://en.wikipedia.org/wiki/Fluorescence_microscope] is_a: ECO:0001098 ! microscopy evidence is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006272 name: viscosity measurement evidence def: "A type of direct assay evidence where the viscosity of a solution is measured to quantify the material properties of the solution, or the changes of these properties in response to changing composition or environmental factors." [url:https\://en.wikipedia.org/wiki/Viscometer] synonym: "viscometry" EXACT [] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006273 name: dynamic fluorescence quenching evidence def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the interaction between a fluorescence donor and acceptor in their excited state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] synonym: "Dexter" NARROW [] synonym: "exciplex" NARROW [] synonym: "FRET" NARROW [] synonym: "quenching" BROAD [] is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006274 name: static fluorescence quenching evidence def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the intramolecular interaction between a fluorescence donor and acceptor creating a non-fluorescent ground state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] synonym: "quenching" BROAD [] is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006275 name: analytical ultracentrifugation evidence used in manual assertion def: "A type of analytical ultracentrifugation evidence that is used in a manual assertion." [ECO:SN] synonym: "AUC" EXACT [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006247 ! analytical ultracentrifugation evidence intersection_of: ECO:0006247 ! analytical ultracentrifugation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006276 name: analytical ultracentrifugation evidence used in automatic assertion def: "A type of analytical ultracentrifugation evidence that is used in an automatic assertion." [ECO:SN] synonym: "AUC" EXACT [] is_a: ECO:0006247 ! analytical ultracentrifugation evidence is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion intersection_of: ECO:0006247 ! analytical ultracentrifugation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006277 name: fluorescence polarization evidence used in manual assertion def: "A type of fluorescence polarization evidence that is used in a manual assertion." [ECO:SN] synonym: "FP" EXACT [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0006248 ! fluorescence polarization evidence intersection_of: ECO:0006248 ! fluorescence polarization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006278 name: fluorescence polarization evidence used in automatic assertion def: "A type of fluorescence polarization evidence that is used in an automatic assertion." [ECO:SN] synonym: "FP" EXACT [] is_a: ECO:0006248 ! fluorescence polarization evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0006248 ! fluorescence polarization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006279 name: bait-prey protein pull-down evidence used in manual assertion def: "A type of bait-prey protein pull-down evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006249 ! bait-prey protein pull-down evidence is_a: ECO:0006392 {is_inferred="true"} ! bait-prey interaction evidence used in manual assertion intersection_of: ECO:0006249 ! bait-prey protein pull-down evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006280 name: bait-prey protein pull-down evidence used in automatic assertion def: "A type of bait-prey protein pull-down evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006249 ! bait-prey protein pull-down evidence is_a: ECO:0006393 {is_inferred="true"} ! bait-prey interaction evidence used in automatic assertion intersection_of: ECO:0006249 ! bait-prey protein pull-down evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006281 name: rotary shadowing electron microscopy evidence used in manual assertion def: "A type of rotary shadowing electron microscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "RS-TEM" EXACT [] is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence intersection_of: ECO:0006250 ! rotary shadowing electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006282 name: rotary shadowing electron microscopy evidence used in automatic assertion def: "A type of rotary shadowing electron microscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "RS-TEM" EXACT [] is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion intersection_of: ECO:0006250 ! rotary shadowing electron microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006283 name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in a manual assertion." [ECO:SN] synonym: "ESI FT-ICR MS" EXACT [] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence intersection_of: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006284 name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in automatic assertion def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] synonym: "ESI FT-ICR MS" EXACT [] is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006285 name: magnetic resonance evidence used in manual assertion def: "A type of magnetic resonance evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006252 ! magnetic resonance evidence intersection_of: ECO:0006252 ! magnetic resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006286 name: magnetic resonance evidence used in automatic assertion def: "A type of magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006252 ! magnetic resonance evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0006252 ! magnetic resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006287 name: electron paramagnetic resonance evidence used in manual assertion def: "A type of electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] synonym: "EPR" EXACT [] synonym: "ESR" EXACT [] is_a: ECO:0006253 ! electron paramagnetic resonance evidence is_a: ECO:0006285 {is_inferred="true"} ! magnetic resonance evidence used in manual assertion intersection_of: ECO:0006253 ! electron paramagnetic resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006288 name: electron paramagnetic resonance evidence used in automatic assertion def: "A type of electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] synonym: "EPR" EXACT [] synonym: "ESR" EXACT [] is_a: ECO:0006253 ! electron paramagnetic resonance evidence is_a: ECO:0006286 {is_inferred="true"} ! magnetic resonance evidence used in automatic assertion intersection_of: ECO:0006253 ! electron paramagnetic resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006289 name: site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] synonym: "SDSL" EXACT [] is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence is_a: ECO:0006287 {is_inferred="true"} ! electron paramagnetic resonance evidence used in manual assertion intersection_of: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006290 name: site-directed spin-labelling electron paramagnetic resonance evidence used in automatic assertion def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] synonym: "SDSL" EXACT [] is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence is_a: ECO:0006288 {is_inferred="true"} ! electron paramagnetic resonance evidence used in automatic assertion intersection_of: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006291 name: deglycosylation assay evidence used in manual assertion def: "A type of deglycosylation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006255 ! deglycosylation assay evidence intersection_of: ECO:0006255 ! deglycosylation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006292 name: deglycosylation assay evidence used in automatic assertion def: "A type of deglycosylation assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006255 ! deglycosylation assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0006255 ! deglycosylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006293 name: protein fragment functional complementation evidence used in manual assertion def: "A type of protein fragment functional complementation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0006256 ! protein fragment functional complementation evidence intersection_of: ECO:0006256 ! protein fragment functional complementation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006294 name: protein fragment functional complementation evidence used in automatic assertion def: "A type of protein fragment functional complementation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006256 ! protein fragment functional complementation evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0006256 ! protein fragment functional complementation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006295 name: beta galactosidase functional complementation evidence used in manual assertion def: "A type of beta galactosidase functional complementation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006257 ! beta galactosidase functional complementation evidence is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion intersection_of: ECO:0006257 ! beta galactosidase functional complementation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006296 name: beta galactosidase functional complementation evidence used in automatic assertion def: "A type of beta galactosidase functional complementation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006257 ! beta galactosidase functional complementation evidence is_a: ECO:0006294 {is_inferred="true"} ! protein fragment functional complementation evidence used in automatic assertion intersection_of: ECO:0006257 ! beta galactosidase functional complementation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006297 name: GAL4-VP16 functional complementation evidence used in manual assertion def: "A type of GAL4-VP16 functional complementation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion intersection_of: ECO:0006258 ! GAL4-VP16 functional complementation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006298 name: GAL4-VP16 functional complementation evidence used in automatic assertion def: "A type of GAL4-VP16 functional complementation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence is_a: ECO:0006294 {is_inferred="true"} ! protein fragment functional complementation evidence used in automatic assertion intersection_of: ECO:0006258 ! GAL4-VP16 functional complementation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006299 name: Raman spectroscopy evidence used in manual assertion def: "A type of Raman spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "Raman optical activity" EXACT [] synonym: "ROA" EXACT [] is_a: ECO:0001258 {is_inferred="true"} ! spectrophotometry evidence used in manual assertion is_a: ECO:0006259 ! Raman spectroscopy evidence intersection_of: ECO:0006259 ! Raman spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006300 name: Raman spectroscopy evidence used in automatic assertion def: "A type of Raman spectroscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "Raman optical activity" EXACT [] synonym: "ROA" EXACT [] is_a: ECO:0006259 ! Raman spectroscopy evidence is_a: ECO:0007458 {is_inferred="true"} ! spectrophotometry evidence used in automatic assertion is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0006259 ! Raman spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006301 name: protein thermal shift assay evidence used in manual assertion def: "A type of protein thermal shift assay evidence that is used in a manual assertion." [ECO:SN] synonym: "TSA" EXACT [] is_a: ECO:0006260 ! protein thermal shift assay evidence is_a: ECO:0006317 {is_inferred="true"} ! temperature-induced protein unfolding evidence used in manual assertion intersection_of: ECO:0006260 ! protein thermal shift assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006302 name: protein thermal shift assay evidence used in automatic assertion def: "A type of protein thermal shift assay evidence that is used in an automatic assertion." [ECO:SN] synonym: "TSA" EXACT [] is_a: ECO:0006260 ! protein thermal shift assay evidence is_a: ECO:0006318 {is_inferred="true"} ! temperature-induced protein unfolding evidence used in automatic assertion intersection_of: ECO:0006260 ! protein thermal shift assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006303 name: microscale thermophoresis evidence used in manual assertion def: "A type of microscale thermophoresis evidence that is used in a manual assertion." [ECO:SN] synonym: "MST" EXACT [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006261 ! microscale thermophoresis evidence intersection_of: ECO:0006261 ! microscale thermophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006304 name: microscale thermophoresis evidence used in automatic assertion def: "A type of microscale thermophoresis evidence that is used in an automatic assertion." [ECO:SN] synonym: "MST" EXACT [] is_a: ECO:0006261 ! microscale thermophoresis evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006261 ! microscale thermophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006305 name: native protein gel electrophoresis evidence used in manual assertion def: "A type of native protein gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] synonym: "BN-PAGE" NARROW [] synonym: "CN-PAGE" NARROW [] synonym: "native gel" EXACT [] synonym: "native PAGE" NARROW [] synonym: "QPNC-PAGE" NARROW [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006262 ! native protein gel electrophoresis evidence intersection_of: ECO:0006262 ! native protein gel electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006306 name: native protein gel electrophoresis evidence used in automatic assertion def: "A type of native protein gel electrophoresis evidence that is used in an automatic assertion." [ECO:SN] synonym: "BN-PAGE" NARROW [] synonym: "CN-PAGE" NARROW [] synonym: "native gel" EXACT [] synonym: "native PAGE" NARROW [] synonym: "QPNC-PAGE" NARROW [] is_a: ECO:0006262 ! native protein gel electrophoresis evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0006262 ! native protein gel electrophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006307 name: turbidity measurement evidence used in manual assertion def: "A type of turbidity measurement evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006263 ! turbidity measurement evidence intersection_of: ECO:0006263 ! turbidity measurement evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006308 name: turbidity measurement evidence used in automatic assertion def: "A type of turbidity measurement evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006263 ! turbidity measurement evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0006263 ! turbidity measurement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006309 name: competitive binding evidence used in manual assertion def: "A type of competitive binding evidence that is used in a manual assertion." [ECO:SN] synonym: "competition assay" EXACT [] is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion is_a: ECO:0006264 ! competitive binding evidence intersection_of: ECO:0006264 ! competitive binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006310 name: competitive binding evidence used in automatic assertion def: "A type of competitive binding evidence that is used in an automatic assertion." [ECO:SN] synonym: "competition assay" EXACT [] is_a: ECO:0006264 ! competitive binding evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0006264 ! competitive binding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006311 name: protein unfolding evidence used in manual assertion def: "A type of protein unfolding evidence that is used in a manual assertion." [ECO:SN] synonym: "protein denaturation assay" EXACT [] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006265 ! protein unfolding evidence intersection_of: ECO:0006265 ! protein unfolding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006312 name: protein unfolding evidence used in automatic assertion def: "A type of protein unfolding evidence that is used in an automatic assertion." [ECO:SN] synonym: "protein denaturation assay" EXACT [] is_a: ECO:0006265 ! protein unfolding evidence is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0006265 ! protein unfolding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006313 name: urea-induced protein unfolding evidence used in manual assertion def: "A type of urea-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] synonym: "chemical denaturation" BROAD [] synonym: "chemical unfolding" BROAD [] is_a: ECO:0006266 ! urea-induced protein unfolding evidence is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion intersection_of: ECO:0006266 ! urea-induced protein unfolding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006314 name: urea-induced protein unfolding evidence used in automatic assertion def: "A type of urea-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] synonym: "chemical denaturation" BROAD [] synonym: "chemical unfolding" BROAD [] is_a: ECO:0006266 ! urea-induced protein unfolding evidence is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion intersection_of: ECO:0006266 ! urea-induced protein unfolding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006315 name: pH-induced protein unfolding evidence used in manual assertion def: "A type of pH-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006267 ! pH-induced protein unfolding evidence is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion intersection_of: ECO:0006267 ! pH-induced protein unfolding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006316 name: pH-induced protein unfolding evidence used in automatic assertion def: "A type of pH-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006267 ! pH-induced protein unfolding evidence is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion intersection_of: ECO:0006267 ! pH-induced protein unfolding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006317 name: temperature-induced protein unfolding evidence used in manual assertion def: "A type of temperature-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] synonym: "thermal dentauration" EXACT [] synonym: "thermal unfolding" EXACT [] is_a: ECO:0006268 ! temperature-induced protein unfolding evidence is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion intersection_of: ECO:0006268 ! temperature-induced protein unfolding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006318 name: temperature-induced protein unfolding evidence used in automatic assertion def: "A type of temperature-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] synonym: "thermal dentauration" EXACT [] synonym: "thermal unfolding" EXACT [] is_a: ECO:0006268 ! temperature-induced protein unfolding evidence is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion intersection_of: ECO:0006268 ! temperature-induced protein unfolding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006319 name: cell aggregation evidence used in manual assertion def: "A type of cell aggregation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006269 ! cell aggregation evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0006269 ! cell aggregation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006320 name: cell aggregation evidence used in automatic assertion def: "A type of cell aggregation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006269 ! cell aggregation evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0006269 ! cell aggregation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006321 name: RNA-protein binding evidence used in manual assertion def: "A type of RNA-protein binding evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion is_a: ECO:0006270 ! RNA-protein binding evidence intersection_of: ECO:0006270 ! RNA-protein binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006322 name: RNA-protein binding evidence used in automatic assertion def: "A type of RNA-protein binding evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006270 ! RNA-protein binding evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0006270 ! RNA-protein binding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006323 name: fluorescence microscopy evidence used in manual assertion def: "A type of fluorescence microscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006271 ! fluorescence microscopy evidence intersection_of: ECO:0006271 ! fluorescence microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006324 name: fluorescence microscopy evidence used in automatic assertion def: "A type of fluorescence microscopy evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006271 ! fluorescence microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006271 ! fluorescence microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006325 name: viscosity measurement evidence used in manual assertion def: "A type of viscosity measurement evidence that is used in a manual assertion." [ECO:SN] synonym: "viscometry" EXACT [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006272 ! viscosity measurement evidence intersection_of: ECO:0006272 ! viscosity measurement evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006326 name: viscosity measurement evidence used in automatic assertion def: "A type of viscosity measurement evidence that is used in an automatic assertion." [ECO:SN] synonym: "viscometry" EXACT [] is_a: ECO:0006272 ! viscosity measurement evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0006272 ! viscosity measurement evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006327 name: dynamic fluorescence quenching evidence used in manual assertion def: "A type of dynamic fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] synonym: "Dexter" NARROW [] synonym: "exciplex" NARROW [] synonym: "FRET" NARROW [] synonym: "quenching" BROAD [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006273 ! dynamic fluorescence quenching evidence intersection_of: ECO:0006273 ! dynamic fluorescence quenching evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006328 name: dynamic fluorescence quenching evidence used in automatic assertion def: "A type of dynamic fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] synonym: "Dexter" NARROW [] synonym: "exciplex" NARROW [] synonym: "FRET" NARROW [] synonym: "quenching" BROAD [] is_a: ECO:0006273 ! dynamic fluorescence quenching evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006273 ! dynamic fluorescence quenching evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006329 name: static fluorescence quenching evidence used in manual assertion def: "A type of static fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] synonym: "quenching" BROAD [] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006274 ! static fluorescence quenching evidence intersection_of: ECO:0006274 ! static fluorescence quenching evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006330 name: static fluorescence quenching evidence used in automatic assertion def: "A type of static fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] synonym: "quenching" BROAD [] is_a: ECO:0006274 ! static fluorescence quenching evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006274 ! static fluorescence quenching evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string created_by: snadendla [Term] id: ECO:0006331 name: Congo red staining evidence def: "A type of staining evidence where Congo red is used to stain cellular structures including amyloid fibrils and aggregates, cell walls of plants and fungi, and for the outer membrane of Gram-negative bacteria." [url:https\://en.wikipedia.org/wiki/Congo_red, url:https\://pubmed.ncbi.nlm.nih.gov/34431623/] synonym: "Congo Red" EXACT [] synonym: "CR" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006332 name: Congo red birefringence evidence def: "A type of Congo red staining evidence where the Congo red dye produces an apple-green birefringence when it specifically binds to cross-β-sheets of amyloids which is detectable via cross-polarized light." [url:https\://pubmed.ncbi.nlm.nih.gov/34431623/] is_a: ECO:0006331 ! Congo red staining evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006333 name: thioflavin T based fluorescence spectroscopy evidence def: "A type of fluorescence evidence used to assess the formation of amyloid fibrils indicated by an increase in Thioflavin T fluorescence intensity and a characteristic red shift in the emission spectrum upon binding to amyloids." [url:https\://pubmed.ncbi.nlm.nih.gov/34431623/] synonym: "ThT" EXACT [] synonym: "ThT assay" EXACT [] synonym: "ThT fluorescence assay" EXACT [] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006334 name: differential scanning fluorimetry evidence def: "A type of protein thermal shift assay evidence where thermal unfolding is monitored by measuring the fluorescence intensity upon interaction with its environment." [url:https\://pubmed.ncbi.nlm.nih.gov/19225610/] synonym: "DSF" EXACT [] is_a: ECO:0001115 ! fluorescence evidence is_a: ECO:0006260 ! protein thermal shift assay evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006335 name: fluorescence correlation spectroscopy evidence def: "A type of fluorescence evidence that measures fluctuations in fluorescence intensity used to detect biophysical properties of single molecules such as diffusion coefficients and hydrodynamic radii." [url:https\://en.wikipedia.org/wiki/Fluorescence_correlation_spectroscopy, url:https\://pubmed.ncbi.nlm.nih.gov/17299036/] synonym: "FSC" EXACT [] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006336 name: intrinsic fluorescence-based protein conformation evidence def: "A type of fluorescence evidence used to study protein conformation by measuring the emissions of aromatic amino acids, specifically tryptophan, tyrosine and, to a minor extent, phenylalanine residues. " [url:https\://en.wikipedia.org/wiki/Fluorescence_spectroscopy] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006337 name: stopped-flow fluorescence spectroscopy evidence def: "A type of fluorescence evidence used to study binding kinetics by rapidly mixing two interaction components and then \"stopping\" the reaction in the observation chamber." [url:https\://doi.org/10.1016/bs.mie.2018.09.026] is_a: ECO:0000021 ! physical interaction evidence is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006338 name: temperature jump relaxation evidence def: "A type of fluorescence evidence where the rates of fast chemical reactions in a solution are measured by the intensity of light absorbed or fluoresced by allowing the reaction mixture to come to an equilibrium, and then perturbing the equilibrium by a rapid temperature rise followed by relaxation of the system to the new equilibrium." [url:https\://en.wikipedia.org/wiki/Temperature_jump, url:https\://iopscience.iop.org/article/10.1088/0022-3735/16/12/003] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006339 name: structural ensemble generation evidence def: "A type of computational structure modeling evidence used to build molecular models of intrinsically disordered proteins to capture the dynamic behaviour of their polypeptide chains. Such tools include EOM, ASTEROIDS and ENSEMBLE." [url:https\://doi.org/10.1007/978-3-319-20164-1_4] is_a: ECO:0006341 ! computational structure modeling evidence property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006340 name: molecular dynamics evidence def: "A type of computational evidence used to study the physical movements of atoms and molecules with empirical molecular mechanics force fields." [url:https\://en.wikipedia.org/wiki/Molecular_dynamics] is_a: ECO:0007672 ! computational evidence property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006341 name: computational structure modeling evidence def: "A type of structure determination evidence in which a computational tool is used to generate a model of structure(s) for a molecule." [ECO:MG] is_a: ECO:0005031 ! structure determination evidence is_a: ECO:0007672 ! computational evidence property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006342 name: qualitative macroscopy evidence def: "A type of macroscopy evidence in which non-numerical observed characteristics or features are captured as text descriptions or categorical assignments." [url:https\://en.wikipedia.org/wiki/Qualitative_property] is_a: ECO:0001094 ! macroscopy evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006343 name: quantitative macroscopy evidence def: "A type of macroscopy evidence in which measurement techniques result in numerical data." [url:https\://en.wikipedia.org/wiki/Quantitative_research] is_a: ECO:0001094 ! macroscopy evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006344 name: sporulation assay evidence def: "A type of direct assay evidence that reflects the presence, absence, and/or amount of spore formation." [url:https\://www.merriam-webster.com/dictionary/sporulation] is_a: ECO:0000002 ! direct assay evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006345 name: asexual sporulation assay evidence def: "A type of sporulation assay evidence that reflects the presence, absence, and/or amount of asexual spore formation." [ECO:MG] is_a: ECO:0006344 ! sporulation assay evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006346 name: sexual sporulation assay evidence def: "A type of sporulation assay evidence that reflects the presence, absence, and/or amount of sexual spore formation." [ECO:MG] is_a: ECO:0006344 ! sporulation assay evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006347 name: host-surrogate penetration assay evidence def: "A type of experimental evidence in which the extent of penetration into a host-surrogate material consisting of a synthetic or other non-living substance is measured." [PHI-base] is_a: ECO:0000006 ! experimental evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006348 name: host penetration assay evidence def: "A type of host colonization assay evidence in which the extent of penetration into host tissue is measured." [PHI-base] is_a: ECO:0001830 ! host colonization assay evidence property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006349 name: iodine-125-labeled ligand binding evidence def: "A type of radioligand binding assay evidence in which iodine-125 label is used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." [PMID:22674160] is_a: ECO:0007008 ! radioligand binding assay evidence property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006350 name: bio-layer interferometry evidence def: "A type of molecule detection assay evidence in which the detection of the interaction between molecules is done without the use of labels and is instead based on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor, where the ligand is immobilized onto a bio compatible biosensor while the analyte is in solution." [url:https\://en.wikipedia.org/wiki/Bio-layer_interferometry] is_a: ECO:0005516 ! molecule detection assay evidence property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006351 name: bait-prey interaction evidence def: "A type of protein binding evidence in which the use of \"bait\" molecules or proteins allows the purification or identification of a binding partner, the \"prey\". " [ECO:MG] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006352 name: Congo red staining evidence used in manual assertion def: "A type of Congo red staining evidence that is used in a manual assertion." [ECO:SN] synonym: "Congo Red" EXACT [] synonym: "CR" EXACT [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006331 ! Congo red staining evidence intersection_of: ECO:0006331 ! Congo red staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006353 name: Congo red staining evidence used in automatic assertion def: "A type of Congo red staining evidence that is used in an automatic assertion." [ECO:SN] synonym: "Congo Red" EXACT [] synonym: "CR" EXACT [] is_a: ECO:0006331 ! Congo red staining evidence is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion intersection_of: ECO:0006331 ! Congo red staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006354 name: Congo red birefringence evidence used in manual assertion def: "A type of Congo red birefringence evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006332 ! Congo red birefringence evidence is_a: ECO:0006352 {is_inferred="true"} ! Congo red staining evidence used in manual assertion intersection_of: ECO:0006332 ! Congo red birefringence evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006355 name: Congo red birefringence evidence used in automatic assertion def: "A type of Congo red birefringence evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006332 ! Congo red birefringence evidence is_a: ECO:0006353 {is_inferred="true"} ! Congo red staining evidence used in automatic assertion intersection_of: ECO:0006332 ! Congo red birefringence evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006356 name: thioflavin T based fluorescence spectroscopy evidence used in manual assertion def: "A type of thioflavin T based fluorescence spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "ThT" EXACT [] synonym: "ThT assay" EXACT [] synonym: "ThT fluorescence assay" EXACT [] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006333 ! thioflavin T based fluorescence spectroscopy evidence intersection_of: ECO:0006333 ! thioflavin T based fluorescence spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006357 name: thioflavin T based fluorescence spectroscopy evidence used in automatic assertion def: "A type of thioflavin T based fluorescence spectroscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "ThT" EXACT [] synonym: "ThT assay" EXACT [] synonym: "ThT fluorescence assay" EXACT [] is_a: ECO:0006333 ! thioflavin T based fluorescence spectroscopy evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006333 ! thioflavin T based fluorescence spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006358 name: differential scanning fluorimetry evidence used in manual assertion def: "A type of differential scanning fluorimetry evidence that is used in a manual assertion." [ECO:SN] synonym: "DSF" EXACT [] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006301 {is_inferred="true"} ! protein thermal shift assay evidence used in manual assertion is_a: ECO:0006334 ! differential scanning fluorimetry evidence intersection_of: ECO:0006334 ! differential scanning fluorimetry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006359 name: differential scanning fluorimetry evidence used in automatic assertion def: "A type of differential scanning fluorimetry evidence that is used in an automatic assertion." [ECO:SN] synonym: "DSF" EXACT [] is_a: ECO:0006302 {is_inferred="true"} ! protein thermal shift assay evidence used in automatic assertion is_a: ECO:0006334 ! differential scanning fluorimetry evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006334 ! differential scanning fluorimetry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006360 name: fluorescence correlation spectroscopy evidence used in manual assertion def: "A type of fluorescence correlation spectroscopy evidence that is used in a manual assertion." [ECO:SN] synonym: "FSC" EXACT [] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006335 ! fluorescence correlation spectroscopy evidence intersection_of: ECO:0006335 ! fluorescence correlation spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006361 name: fluorescence correlation spectroscopy evidence used in automatic assertion def: "A type of fluorescence correlation spectroscopy evidence that is used in an automatic assertion." [ECO:SN] synonym: "FSC" EXACT [] is_a: ECO:0006335 ! fluorescence correlation spectroscopy evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006335 ! fluorescence correlation spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006362 name: intrinsic fluorescence-based protein conformation evidence used in manual assertion def: "A type of intrinsic fluorescence-based protein conformation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006336 ! intrinsic fluorescence-based protein conformation evidence intersection_of: ECO:0006336 ! intrinsic fluorescence-based protein conformation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006363 name: intrinsic fluorescence-based protein conformation evidence used in automatic assertion def: "A type of intrinsic fluorescence-based protein conformation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006336 ! intrinsic fluorescence-based protein conformation evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006336 ! intrinsic fluorescence-based protein conformation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006364 name: stopped-flow fluorescence spectroscopy evidence used in manual assertion def: "A type of stopped-flow fluorescence spectroscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006337 ! stopped-flow fluorescence spectroscopy evidence intersection_of: ECO:0006337 ! stopped-flow fluorescence spectroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006365 name: stopped-flow fluorescence spectroscopy evidence used in automatic assertion def: "A type of stopped-flow fluorescence spectroscopy evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006337 ! stopped-flow fluorescence spectroscopy evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006337 ! stopped-flow fluorescence spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006366 name: temperature jump relaxation evidence used in manual assertion def: "A type of temperature jump relaxation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0006338 ! temperature jump relaxation evidence intersection_of: ECO:0006338 ! temperature jump relaxation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006367 name: temperature jump relaxation evidence used in automatic assertion def: "A type of temperature jump relaxation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006338 ! temperature jump relaxation evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0006338 ! temperature jump relaxation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string created_by: snadendla [Term] id: ECO:0006368 name: computational structure modeling evidence used in manual assertion def: "A type of computational structure modeling evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006341 ! computational structure modeling evidence is_a: ECO:0007668 {is_inferred="true"} ! computational evidence used in manual assertion intersection_of: ECO:0006341 ! computational structure modeling evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006369 name: computational structure modeling evidence used in automatic assertion def: "A type of computational structure modeling evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006341 ! computational structure modeling evidence is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0006341 ! computational structure modeling evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006370 name: structural ensemble generation evidence used in manual assertion def: "A type of structural ensemble generation evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006339 ! structural ensemble generation evidence is_a: ECO:0006368 {is_inferred="true"} ! computational structure modeling evidence used in manual assertion intersection_of: ECO:0006339 ! structural ensemble generation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006371 name: structural ensemble generation evidence used in automatic assertion def: "A type of structural ensemble generation evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006339 ! structural ensemble generation evidence is_a: ECO:0006369 {is_inferred="true"} ! computational structure modeling evidence used in automatic assertion intersection_of: ECO:0006339 ! structural ensemble generation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006372 name: molecular dynamics evidence used in manual assertion def: "A type of molecular dynamics evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006340 ! molecular dynamics evidence is_a: ECO:0007668 {is_inferred="true"} ! computational evidence used in manual assertion intersection_of: ECO:0006340 ! molecular dynamics evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006373 name: molecular dynamics evidence used in automatic assertion def: "A type of molecular dynamics evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006340 ! molecular dynamics evidence is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion intersection_of: ECO:0006340 ! molecular dynamics evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 DisProt:EdoardoSalladini xsd:string property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string created_by: snadendla [Term] id: ECO:0006374 name: qualitative macroscopy evidence used in manual assertion def: "A type of qualitative macroscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001228 {is_inferred="true"} ! macroscopy evidence used in manual assertion is_a: ECO:0006342 ! qualitative macroscopy evidence intersection_of: ECO:0006342 ! qualitative macroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006375 name: qualitative macroscopy evidence used in automatic assertion def: "A type of qualitative macroscopy evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006342 ! qualitative macroscopy evidence is_a: ECO:0007430 {is_inferred="true"} ! macroscopy evidence used in automatic assertion intersection_of: ECO:0006342 ! qualitative macroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006376 name: quantitative macroscopy evidence used in manual assertion def: "A type of quantitative macroscopy evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001228 {is_inferred="true"} ! macroscopy evidence used in manual assertion is_a: ECO:0006343 ! quantitative macroscopy evidence intersection_of: ECO:0006343 ! quantitative macroscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006377 name: quantitative macroscopy evidence used in automatic assertion def: "A type of quantitative macroscopy evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006343 ! quantitative macroscopy evidence is_a: ECO:0007430 {is_inferred="true"} ! macroscopy evidence used in automatic assertion intersection_of: ECO:0006343 ! quantitative macroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006378 name: sporulation assay evidence used in manual assertion def: "A type of sporulation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006344 ! sporulation assay evidence intersection_of: ECO:0006344 ! sporulation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006379 name: sporulation assay evidence used in automatic assertion def: "A type of sporulation assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006344 ! sporulation assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0006344 ! sporulation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006380 name: asexual sporulation assay evidence used in manual assertion def: "A type of asexual sporulation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006345 ! asexual sporulation assay evidence is_a: ECO:0006378 {is_inferred="true"} ! sporulation assay evidence used in manual assertion intersection_of: ECO:0006345 ! asexual sporulation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006381 name: asexual sporulation assay evidence used in automatic assertion def: "A type of asexual sporulation assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006345 ! asexual sporulation assay evidence is_a: ECO:0006379 {is_inferred="true"} ! sporulation assay evidence used in automatic assertion intersection_of: ECO:0006345 ! asexual sporulation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006382 name: sexual sporulation assay evidence used in manual assertion def: "A type of sexual sporulation assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006346 ! sexual sporulation assay evidence is_a: ECO:0006378 {is_inferred="true"} ! sporulation assay evidence used in manual assertion intersection_of: ECO:0006346 ! sexual sporulation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006383 name: sexual sporulation assay evidence used in automatic assertion def: "A type of sexual sporulation assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006346 ! sexual sporulation assay evidence is_a: ECO:0006379 {is_inferred="true"} ! sporulation assay evidence used in automatic assertion intersection_of: ECO:0006346 ! sexual sporulation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006384 name: host-surrogate penetration assay evidence used in manual assertion def: "A type of host-surrogate penetration assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006347 ! host-surrogate penetration assay evidence intersection_of: ECO:0006347 ! host-surrogate penetration assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006385 name: host-surrogate penetration assay evidence used in automatic assertion def: "A type of host-surrogate penetration assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006347 ! host-surrogate penetration assay evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0006347 ! host-surrogate penetration assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006386 name: host penetration assay evidence used in manual assertion def: "A type of host penetration assay evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0001832 {is_inferred="true"} ! host colonization assay evidence used in manual assertion is_a: ECO:0006348 ! host penetration assay evidence intersection_of: ECO:0006348 ! host penetration assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006387 name: host penetration assay evidence used in automatic assertion def: "A type of host penetration assay evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0001831 {is_inferred="true"} ! host colonization assay evidence used in automatic assertion is_a: ECO:0006348 ! host penetration assay evidence intersection_of: ECO:0006348 ! host penetration assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 PHI-base:AlayneCuzick xsd:string created_by: snadendla [Term] id: ECO:0006388 name: iodine-125-labeled ligand binding evidence used in manual assertion def: "A type of iodine-125-labeled ligand binding evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006349 ! iodine-125-labeled ligand binding evidence is_a: ECO:0007009 {is_inferred="true"} ! radioligand binding assay evidence used in manual assertion intersection_of: ECO:0006349 ! iodine-125-labeled ligand binding evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006389 name: iodine-125-labeled ligand binding evidence used in automatic assertion def: "A type of iodine-125-labeled ligand binding evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006349 ! iodine-125-labeled ligand binding evidence is_a: ECO:0007591 {is_inferred="true"} ! radioligand binding assay evidence used in automatic assertion intersection_of: ECO:0006349 ! iodine-125-labeled ligand binding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006390 name: bio-layer interferometry evidence used in manual assertion def: "A type of bio-layer interferometry evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0006350 ! bio-layer interferometry evidence intersection_of: ECO:0006350 ! bio-layer interferometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006391 name: bio-layer interferometry evidence used in automatic assertion def: "A type of bio-layer interferometry evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006350 ! bio-layer interferometry evidence is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion intersection_of: ECO:0006350 ! bio-layer interferometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006392 name: bait-prey interaction evidence used in manual assertion def: "A type of bait-prey interaction evidence that is used in a manual assertion." [ECO:SN] is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion is_a: ECO:0006351 ! bait-prey interaction evidence intersection_of: ECO:0006351 ! bait-prey interaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0006393 name: bait-prey interaction evidence used in automatic assertion def: "A type of bait-prey interaction evidence that is used in an automatic assertion." [ECO:SN] is_a: ECO:0006351 ! bait-prey interaction evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion intersection_of: ECO:0006351 ! bait-prey interaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string property_value: IAO:0000234 MimicDB:KatalinParej xsd:string created_by: snadendla [Term] id: ECO:0007000 name: high throughput mutant phenotypic evidence def: "A type of high throughput evidence derived from high throughput analysis of differences in alleles of a corresponding gene." [GO:IMP] synonym: "high throughput mutant phenotype evidence" EXACT [] is_a: ECO:0000015 ! mutant phenotype evidence is_a: ECO:0006055 ! high throughput evidence property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007001 name: high throughput mutant phenotypic evidence used in manual assertion def: "A type of high throughput mutant phenotypic evidence that is used in a manual assertion." [GO:IMP] comment: When using the HMP evidence code, the guidelines for IMP should be adhered to (http://geneontology.org/page/imp-inferred-mutant-phenotype) synonym: "high throughput mutant phenotype evidence used in manual assertion" EXACT [] synonym: "HMP" EXACT [GOECO:HMP] synonym: "inferred from high throughput mutant phenotype" EXACT [GOECO:HMP] xref: GOECO:HMP "inferred from high throughput mutant phenotype" is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion is_a: ECO:0007000 ! high throughput mutant phenotypic evidence intersection_of: ECO:0007000 ! high throughput mutant phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HMP" xsd:string {comment="Default"} property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007002 name: high throughput genetic interaction phenotypic evidence def: "A type of high throughput evidence resulting from the high throughput analysis of the effect that a given gene has on another gene or genes, and products." [PMID:11822023] synonym: "high throughput genetic interaction evidence" EXACT [] is_a: ECO:0000011 ! genetic interaction evidence is_a: ECO:0006055 ! high throughput evidence property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007003 name: high throughput genetic interaction phenotypic evidence used in manual assertion def: "A type of high throughput genetic interaction phenotypic evidence that is used in a manual assertion." [PMID:11822023] comment: When using the HGI evidence code, the guidelines for IGI should be adhered to (http://geneontology.org/page/igi-inferred-genetic-interaction) synonym: "HGI" EXACT [GOECO:HGI] synonym: "high throughput genetic interaction evidence used in manual assertion" EXACT [] synonym: "inferred from high throughput genetic interaction" EXACT [GOECO:HGI] xref: GOECO:HGI "inferred from high throughput genetic interaction" is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion is_a: ECO:0007002 ! high throughput genetic interaction phenotypic evidence intersection_of: ECO:0007002 ! high throughput genetic interaction phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HGI" xsd:string {comment="Default"} property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007004 name: high throughput direct assay evidence def: "A type of high throughput evidence derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] is_a: ECO:0000002 ! direct assay evidence is_a: ECO:0006055 ! high throughput evidence property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007005 name: high throughput direct assay evidence used in manual assertion def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] comment: When using the HDA evidence code, the guidelines for IDA should be adhered to (http://geneontology.org/page/ida-inferred-direct-assay) synonym: "HDA" EXACT [GOECO:HDA] synonym: "inferred from high throughput direct assay" EXACT [GOECO:HDA] xref: GOECO:HDA "inferred from high throughput direct assay" is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion is_a: ECO:0007004 ! high throughput direct assay evidence intersection_of: ECO:0007004 ! high throughput direct assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HDA" xsd:string {comment="Default"} property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007006 name: high throughput expression pattern evidence def: "A type of high throughput evidence derived from the high throughput characterization of gene expression." [GO:IEP] is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0006055 ! high throughput evidence property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007007 name: high throughput expression pattern evidence used in manual assertion def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput characterization of gene expression." [GO:IEP] comment: When using the HEP evidence code, the guidelines for EXP should be adhered to (http://geneontology.org/page/iep-inferred-expression-pattern) synonym: "HEP" EXACT [GOECO:HEP] synonym: "inferred from high throughput expression pattern" EXACT [GOECO:HEP] xref: GOECO:HEP "inferred from high throughput expression pattern" is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion is_a: ECO:0007006 ! high throughput expression pattern evidence intersection_of: ECO:0007006 ! high throughput expression pattern evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "HEP" xsd:string {comment="Default"} property_value: IAO:0000234 "GOC" xsd:string created_by: rctauber creation_date: 2017-10-05T07:39:47Z [Term] id: ECO:0007008 name: radioligand binding assay evidence def: "A type of protein binding evidence in which radioactive ligands are used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." [PMID:27471749] synonym: "competitive binding assay evidence" NARROW [] synonym: "radioactive ligand binding assay evidence" EXACT [] synonym: "radiometric ligand-binding assay evidence" EXACT [] is_a: ECO:0000024 ! protein binding evidence property_value: IAO:0000234 "fjungo" xsd:string created_by: rctauber creation_date: 2017-12-15T10:46:19Z [Term] id: ECO:0007009 name: radioligand binding assay evidence used in manual assertion def: "A type of radioligand binding assay evidence that is used in a manual assertion." [ECO:RCT] synonym: "competitive binding assay evidence" RELATED [] synonym: "radioactive ligand binding assay evidence" BROAD [] is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion is_a: ECO:0007008 ! radioligand binding assay evidence intersection_of: ECO:0007008 ! radioligand binding assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2017-12-15T10:48:44Z [Term] id: ECO:0007011 name: combinatorial experimental and author inference evidence def: "A type of combinatorial evidence in which the author draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] synonym: "combinatorial evidence from author knowledge and experimental evidence" EXACT [] is_a: ECO:0000212 {is_inferred="true"} ! combinatorial evidence intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence intersection_of: BFO:0000051 ECO:0006185 ! author inference property_value: IAO:0000234 "sbello" xsd:string created_by: rctauber creation_date: 2018-02-15T11:17:11Z [Term] id: ECO:0007012 name: combinatorial experimental and curator inference evidence def: "A type of combinatorial evidence in which the curator draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] synonym: "combinatorial evidence from curator knowledge and experimental evidence" EXACT [] is_a: ECO:0000212 {is_inferred="true"} ! combinatorial evidence intersection_of: ECO:0000212 ! combinatorial evidence intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence intersection_of: BFO:0000051 ECO:0000205 ! curator inference property_value: IAO:0000234 "ZFIN" xsd:string created_by: rctauber creation_date: 2018-02-15T11:17:11Z [Term] id: ECO:0007013 name: combinatorial experimental and author inference evidence used in manual assertion def: "A type of combinatorial evidence from author knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] synonym: "combinatorial evidence from author knowledge and experimental evidence used in manual assertion" EXACT [] is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion is_a: ECO:0007011 ! combinatorial experimental and author inference evidence intersection_of: ECO:0007011 ! combinatorial experimental and author inference evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-02-15T11:22:10Z [Term] id: ECO:0007014 name: combinatorial experimental and curator inference evidence used in manual assertion def: "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] synonym: "combinatorial evidence from curator knowledge and experimental evidence used in manual assertion" EXACT [] is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion is_a: ECO:0007012 ! combinatorial experimental and curator inference evidence intersection_of: ECO:0007012 ! combinatorial experimental and curator inference evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-02-15T11:22:10Z [Term] id: ECO:0007015 name: voltammetry evidence def: "A type of substance quantification evidence where an electrical gradient potential is applied to analyze an analyte of interest, resulting in a measurement amount of electrical current across the potential range applied." [PMID:28127962] is_a: ECO:0000335 ! substance quantification evidence property_value: IAO:0000234 "SynGO" xsd:string created_by: rctauber creation_date: 2018-02-28T12:11:00Z [Term] id: ECO:0007016 name: voltammetry evidence used in manual assertion def: "A type of voltammetry evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0007015 ! voltammetry evidence intersection_of: ECO:0007015 ! voltammetry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-02-28T12:12:34Z [Term] id: ECO:0007017 name: photoconversion evidence def: "A type of fluorescence evidence where a molecule tagged with a fluorescent protein is exposed to ultraviolet or blue light to shift the spectral emission properties for visualization." [ECO:RCT, PMID:28574633] is_a: ECO:0001115 ! fluorescence evidence property_value: IAO:0000234 "SynGO" xsd:string created_by: rctauber creation_date: 2018-03-02T09:01:00Z [Term] id: ECO:0007018 name: photoconversion evidence used in manual assertion def: "A type of photoconversion evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion is_a: ECO:0007017 ! photoconversion evidence intersection_of: ECO:0007017 ! photoconversion evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-03-02T09:05:04Z [Term] id: ECO:0007019 name: agglutination test evidence def: "A type of immunological assay evidence that involves observation of visible clumping of antibody and antigen into a complex." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] synonym: "agglutination assay evidence" EXACT [] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007020 name: agglutination test evidence used in manual assertion def: "A type of agglutination test evidence that is used in a manual assertion." [ECO:RCT] synonym: "agglutination assay evidence" BROAD [] is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion is_a: ECO:0007019 ! agglutination test evidence intersection_of: ECO:0007019 ! agglutination test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007021 name: slide agglutination test evidence def: "A type of agglutination test evidence that involves observaiton of visible clumping of antibody and antigen into a complex on a slide." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] is_a: ECO:0007019 ! agglutination test evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007022 name: slide agglutination test evidence used in manual assertion def: "A type of slide agglutination test evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion is_a: ECO:0007021 ! slide agglutination test evidence intersection_of: ECO:0007021 ! slide agglutination test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007023 name: direct Coombs test evidence def: "A type of agglutination test evidence that results in detection of nonagglutinating antibodies or complement proteins on red blood cells in vivo." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] synonym: "direct antihuman globulin test (DAT)" EXACT [] is_a: ECO:0007019 ! agglutination test evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007024 name: direct Coombs test evidence used in manual assertion def: "A type of direct Coombs test evidence that is used in a manual assertion." [ECO:RCT] synonym: "direct antihuman globulin test (DAT)" BROAD [] is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion is_a: ECO:0007023 ! direct Coombs test evidence intersection_of: ECO:0007023 ! direct Coombs test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007025 name: indirect Coombs test evidence def: "A type of agglutination test evidence that results in screening of antibodies against red blood cell antigens (other than the A and B antigens) that are unbound in a patient's serum in vitro." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] synonym: "indirect antiglobulin test (IAT)" EXACT [] is_a: ECO:0007019 ! agglutination test evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007026 name: indirect Coombs test evidence used in manual assertion def: "A type of indirect Coombs test evidence that is used in a manual assertion." [ECO:RCT] synonym: "indirect antiglobulin test (IAT)" BROAD [] is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion is_a: ECO:0007025 ! indirect Coombs test evidence intersection_of: ECO:0007025 ! indirect Coombs test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007027 name: direct hemagglutination assay evidence def: "A type of agglutination test evidence where some bacteria and viruses cross-link red blood cells and clump together." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] is_a: ECO:0007019 ! agglutination test evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007028 name: direct hemagglutination assay evidence used in manual assertion def: "A type of direct hemagglutination assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion is_a: ECO:0007027 ! direct hemagglutination assay evidence intersection_of: ECO:0007027 ! direct hemagglutination assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007029 name: viral hemagglutination inhibition assay evidence def: "A type of agglutination test evidence where antiviral antibodies in a patient's serum or in a lab-produced antiserum neutralize the virus and block it from agglutinating the red blood cells." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] is_a: ECO:0007019 ! agglutination test evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007030 name: viral hemagglutination inhibition assay evidence used in manual assertion def: "A type of viral hemagglutination inhibition assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion is_a: ECO:0007029 ! viral hemagglutination inhibition assay evidence intersection_of: ECO:0007029 ! viral hemagglutination inhibition assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007031 name: compement fixation assay evidence def: "A type of immunological assay evidence that results in the detection of presence of specific antibodies in the patient's serum based on the use of complement, a biologically labile serum factor." [URL:http\://laboratoryinfo.com/complement-fixation-test/] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007032 name: compement fixation assay evidence used in manual assertion def: "A type of compement fixation assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion is_a: ECO:0007031 ! compement fixation assay evidence intersection_of: ECO:0007031 ! compement fixation assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007033 name: neutralization test assay evidence def: "A type of immunogical assay evidence that results in detection of specific neutralizing antibodies in a sample." [PMID:24899440, url:http\://www.creative-biolabs.com/drug-discovery/therapeutics/neutralization-assay.htm?gclid=Cj0KEQjwqvvLBRDIt-D7q7iqiOcBEiQAxi68ERRz00a0x9sWSKSLDu7d5-kMi4AdiLNdUq1mXzePdywaArli8P8HAQ)] is_a: ECO:0000040 ! immunological assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007034 name: neutralization test assay evidence used in manual assertion def: "A type of neutralization test assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion is_a: ECO:0007033 ! neutralization test assay evidence intersection_of: ECO:0007033 ! neutralization test assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007035 name: copper transport assay evidence def: "A type of transport assay evidence where the rate of copper transport is estimated." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689948/] is_a: ECO:0000134 ! transport assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007036 name: copper transport assay evidence used in manual assertion def: "A type of copper transport assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0007035 ! copper transport assay evidence intersection_of: ECO:0007035 ! copper transport assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007037 name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence def: "A type of staining evidence that involves the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride to evaluate the respiratory activity of cells." [PMID:1622256] synonym: "CTC staining" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007038 name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion def: "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in a manual assertion." [ECO:RCT] synonym: "CTC staining" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence intersection_of: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007039 name: plaque assay evidence def: "A type of cell growth assay evidence resulting in direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture." [PMID:25407402] is_a: ECO:0001563 ! cell growth assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007040 name: plaque assay evidence used in manual assertion def: "A type of plaque assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007039 ! plaque assay evidence is_a: ECO:0007699 {is_inferred="true"} ! cell growth assay evidence used in manual assertion intersection_of: ECO:0007039 ! plaque assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007041 name: epifluorescence microscopy evidence def: "A type of fluorescence microscopy evidence where the light source is mounted above the specimen and the excitation light passes through the microscope objective lens on its way toward the specimen." [URL:http\://www.rsc.org/publishing/journals/prospect/ontology.asp?id=CMO\:0001096|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713126/] is_a: ECO:0006271 ! fluorescence microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007042 name: epifluorescence microscopy evidence used in manual assertion def: "A type of epifluorescence microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006323 {is_inferred="true"} ! fluorescence microscopy evidence used in manual assertion is_a: ECO:0007041 ! epifluorescence microscopy evidence intersection_of: ECO:0007041 ! epifluorescence microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007043 name: transmission electron microscopy evidence def: "A type of electron microscopy evidence where a beam of electrons are transmitted through an ultra thin specimen forming an image which is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera." [ERO:0000329|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/] synonym: "conventional transmission electron microscopy(CTEM)" EXACT [] synonym: "TEM" EXACT [] is_a: ECO:0005033 ! electron microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007044 name: transmission electron microscopy evidence used in manual assertion def: "A type of transmission electron microscopy evidence that is used in a manual assertion." [ECO:RCT] synonym: "conventional transmission electron microscopy(CTEM)" BROAD [] synonym: "TEM" BROAD [] is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion is_a: ECO:0007043 ! transmission electron microscopy evidence intersection_of: ECO:0007043 ! transmission electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007045 name: scanning electron microscopy evidence def: "A type of electron microscopy evidence where a focused beam of high-energy electrons is used to generate a variety of signals at the surface of solid specimens." [URL:https\://serc.carleton.edu/research_education/geochemsheets/techniques/SEM.html] is_a: ECO:0005033 ! electron microscopy evidence property_value: IAO:0000112 "With a general growth of the recombinant mycobacterial strains resulting in minimal change, the cell morphology was further examined using the scanning electron microscopy (SEM) technique. As shown in Fig. 5B, the cell lengthened when 20 ng/mL tetracycline was added to the medium to induce expression of the antisense mtrA mRNA (right panel)." xsd:string {xref="PMID:20843371"} property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007046 name: scanning electron microscopy evidence used in manual assertion def: "A type of scanning electron microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion is_a: ECO:0007045 ! scanning electron microscopy evidence intersection_of: ECO:0007045 ! scanning electron microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007047 name: time-lapsed microscopy evidence def: "A type of microscopy evidence where a serial images are taken at regular time points to capture the dynamics of what is being observed." [URL:https\://www.nature.com/subjects/time-lapse-imaging] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007048 name: time-lapsed microscopy evidence used in manual assertion def: "A type of time-lapsed microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0007047 ! time-lapsed microscopy evidence intersection_of: ECO:0007047 ! time-lapsed microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007049 name: phase contrast microscopy evidence def: "A type of microscopy evidence where a transparent specimen is illuminated with visible light and small phase shifts in the light passing through the specimen are used to produce an image." [CHMO:0000110] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007050 name: phase contrast microscopy evidence used in manual assertion def: "A type of phase contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0007049 ! phase contrast microscopy evidence intersection_of: ECO:0007049 ! phase contrast microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007051 name: transmitted light brightfied mircoscopy evidence def: "A type of microscopy evidence where a sample is illuminated with transmitted white light from below and observed from above." [BAO:0000457] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007052 name: transmitted light brightfied mircoscopy evidence used in manual assertion def: "A type of transmitted light brightfied mircoscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0007051 ! transmitted light brightfied mircoscopy evidence intersection_of: ECO:0007051 ! transmitted light brightfied mircoscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007053 name: koehler illumination microscopy evidence def: "A type of microscopy evidence that results in a bright image without glare and minimum heating of the specimen by employing both field and an aperture iris diaphragm for illumination." [URL:http\://www.gonda.ucla.edu/bri_core/kohler.htm] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007054 name: koehler illumination microscopy evidence used in manual assertion def: "A type of koehler illumination microscopy evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0007053 ! koehler illumination microscopy evidence intersection_of: ECO:0007053 ! koehler illumination microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007055 name: differential interference contrast microscopy evidence def: "A type of microscopy evidence that results in visualization of living cells and transparent specimens by taking advantage of differences in the light refraction of different parts of the specimen." [URL:http\://www.microscopemaster.com/differential-interference-contrast.html] synonym: "DIC microscopy" EXACT [] synonym: "NIC microscopy" EXACT [] synonym: "Nomarski interference contrast microscopy" EXACT [] synonym: "Nomarski microscopy" EXACT [] is_a: ECO:0001098 ! microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007056 name: differential interference contrast microscopy evidence used in manual assertion def: "A type of differential interference contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] synonym: "DIC microscopy" BROAD [] synonym: "NIC microscopy" BROAD [] synonym: "Nomarski interference contrast microscopy" BROAD [] synonym: "Nomarski microscopy" BROAD [] is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion is_a: ECO:0007055 ! differential interference contrast microscopy evidence intersection_of: ECO:0007055 ! differential interference contrast microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007057 name: extended field laser confocal microscopy evidence def: "A type of confocal microscopy evidence that results in creation of a continuous confocal multi-colour mosaic from thousands of individually captured images." [PMID:18627634] synonym: "EFLCM" EXACT [] is_a: ECO:0005587 ! confocal microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007058 name: extended field laser confocal microscopy evidence used in manual assertion def: "A type of extended field laser confocal microscopy evidence that is used in a manual assertion." [ECO:RCT] synonym: "EFLCM" BROAD [] is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion is_a: ECO:0007057 ! extended field laser confocal microscopy evidence intersection_of: ECO:0007057 ! extended field laser confocal microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007059 name: confocal laser scanning microscopy evidence def: "A type of confocal microscopy evidence that results in an image that is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample.\n" [URL:http\://bitesizebio.com/19958/what-is-confocal-laser-scanning-microscopy/] synonym: "CLSM" EXACT [] synonym: "confocal laser scanning fluorescence microscopy" EXACT [] synonym: "confocal-laser scanning microscopy" EXACT [] synonym: "fluorescence confocal scanning laser microscopy" EXACT [] synonym: "LSCM" EXACT [] synonym: "scanning confocal fluorescence microscopy" EXACT [] is_a: ECO:0005587 ! confocal microscopy evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007060 name: confocal laser scanning microscopy evidence used in manual assertion def: "A type of confocal laser scanning microscopy evidence that is used in a manual assertion." [ECO:RCT] synonym: "CLSM" BROAD [] synonym: "confocal laser scanning fluorescence microscopy" BROAD [] synonym: "confocal-laser scanning microscopy" BROAD [] synonym: "fluorescence confocal scanning laser microscopy" BROAD [] synonym: "LSCM" BROAD [] synonym: "scanning confocal fluorescence microscopy" BROAD [] is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion is_a: ECO:0007059 ! confocal laser scanning microscopy evidence intersection_of: ECO:0007059 ! confocal laser scanning microscopy evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007061 name: light scattering evidence def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by illuminating the sample with a laser beam and the scattered intensity is probed at a certain angle by a detector." [PMID:9013660, url:http\://www.lsinstruments.ch/technology/dynamic_light_scattering_dls/, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] is_a: ECO:0005031 ! structure determination evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007062 name: light scattering evidence used in manual assertion def: "A type of light scattering evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0007061 ! light scattering evidence intersection_of: ECO:0007061 ! light scattering evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007063 name: dynamic light scattering assay evidence def: "A type of light scattering evidence derived by analyzing the fluctuations in the internsity of the scattered light by the particles in motion." [PMID:9013660, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] synonym: "Photon Correlation Spectroscopy" EXACT [] synonym: "Quasi-Elastic Light Scattering" EXACT [] is_a: ECO:0007061 ! light scattering evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007064 name: dynamic light scattering assay evidence used in manual assertion def: "A type of dynamic light scattering assay evidence that is used in a manual assertion." [ECO:RCT] synonym: "Photon Correlation Spectroscopy" BROAD [] synonym: "Quasi-Elastic Light Scattering" BROAD [] is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion is_a: ECO:0007063 ! dynamic light scattering assay evidence intersection_of: ECO:0007063 ! dynamic light scattering assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007065 name: static light scattering assay evidence def: "A type of light scattering evidence derived by measuring the average intensity of scattered light by the particles at multiple angles." [PMID:9013660] synonym: "Rayleigh scattering" EXACT [] is_a: ECO:0007061 ! light scattering evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007066 name: static light scattering assay evidence used in manual assertion def: "A type of static light scattering assay evidence that is used in a manual assertion." [ECO:RCT] synonym: "Rayleigh scattering" BROAD [] is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion is_a: ECO:0007065 ! static light scattering assay evidence intersection_of: ECO:0007065 ! static light scattering assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007067 name: colony papillation assay phenotypic evidence def: "A type of colony morphology phenotypic evidence resulting from the formation of papillae (microcolonies) that protrude outwards from the main colony by mutant cells." [PMID:27447898] synonym: "colony papillation assay evidence" EXACT [] is_a: ECO:0007077 ! colony morphology phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007068 name: colony papillation assay phenotypic evidence used in manual assertion def: "A type of colony papillation assay phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "colony papillation assay evidence used in manual assertion" EXACT [] is_a: ECO:0007067 ! colony papillation assay phenotypic evidence is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion intersection_of: ECO:0007067 ! colony papillation assay phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007069 name: crystal violet staining evidence def: "A type of staining evidence that is derived from the use of gentian violet as a general biological stain and an acid-base indicator." [BAO:0002468] synonym: "gentian violet" EXACT [] synonym: "hexamethyl pararosaniline chloride" EXACT [] synonym: "methyl violet 10B" EXACT [] is_a: ECO:0005019 ! staining evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007070 name: crystal violet staining evidence used in manual assertion def: "A type of crystal violet staining evidence that is used in a manual assertion." [ECO:RCT] synonym: "gentian violet" BROAD [] synonym: "hexamethyl pararosaniline chloride" BROAD [] synonym: "methyl violet 10B" BROAD [] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0007069 ! crystal violet staining evidence intersection_of: ECO:0007069 ! crystal violet staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007071 name: flow cell biofilm assay evidence def: "A type of biofilm formation assay evidence derived from in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438488/] is_a: ECO:0006020 ! biofilm formation assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007072 name: flow cell biofilm assay evidence used in manual assertion def: "A type of flow cell biofilm assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion is_a: ECO:0007071 ! flow cell biofilm assay evidence intersection_of: ECO:0007071 ! flow cell biofilm assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007073 name: bacterial 2-hybrid assay evidence def: "A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the \"bait\") to RNA polymerase and fusing protein or peptide library to be analyzed (the \"prey\") to DNA-binding domain." [URL:http\://www.pnas.org/content/97/13/7382.full.pdf] is_a: ECO:0000025 ! bait-prey hybrid interaction evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007074 name: bacterial 2-hybrid assay evidence used in manual assertion def: "A type of bacterial 2-hybrid assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion is_a: ECO:0007073 ! bacterial 2-hybrid assay evidence intersection_of: ECO:0007073 ! bacterial 2-hybrid assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007075 name: phenomic profiling assay evidence def: "A type of experimental phenotypic evidence that results from the comparison of phenotype profiles (the physical and biochemical traits of organisms in particular environments) across multiple strains; that is, a meta-analysis of a set of phenotypic patterns from many tests in response to a series of changing genetic and/or environmental factors." [EDAM:topic_3298, PMID:2118507] comment: A phenome is defined as the SET of physical and biochemical traits, or, as the SET of all phenotypes expressed, i.e. a phenome is defined as the sum total of all phenotypic traits. comment: D.S. notes: I think this term is meant to be used for high throughput experiments where a large number of mutants is screened for their ability to grow in a large number of conditions. The individual mutants can then clustered based on the similarity of their phenotypic profiles-whether they grew or didn't grow across all the conditions. The goal being to try to identify genes whose products function in the same biological pathways based on their having similar phenotypes across multiple conditions. is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007076 name: phenomic profiling assay evidence used in manual assertion def: "A type of phenomic profiling assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007075 ! phenomic profiling assay evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0007075 ! phenomic profiling assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007077 name: colony morphology phenotypic evidence def: "A type of experimental phenotypic evidence resulting from studying the physical characteristics of an assemblage of microorganisms growing on a solid surface such as the surface of an agar culture medium." [OMP:0000100] synonym: "colony morphology evidence" EXACT [] is_a: ECO:0000059 ! experimental phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007078 name: colony morphology phenotypic evidence used in manual assertion def: "A type of colony morphology phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "colony morphology evidence used in manual assertion" EXACT [] is_a: ECO:0007077 ! colony morphology phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0007077 ! colony morphology phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007079 name: colony color phenotypic evidence def: "A type of colony morphology evidence resulting from studying the colony pigmentation." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671912/] synonym: "colony color evidence" EXACT [] synonym: "colony pigmentation" EXACT [] is_a: ECO:0007077 ! colony morphology phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007080 name: colony color phenotypic evidence used in manual assertion def: "A type of colony color phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "colony color evidence used in manual assertion" EXACT [] synonym: "colony pigmentation" BROAD [] is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion is_a: ECO:0007079 ! colony color phenotypic evidence intersection_of: ECO:0007079 ! colony color phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007081 name: colony size phenotypic evidence def: "A type of colony morphology phenotypic evidence resulting from measuring the area or diameter of the colony." [URL:http\://www.nature.com/nrmicro/journal/v4/n4/full/nrmicro1384.html] synonym: "colony size evidence" EXACT [] is_a: ECO:0007077 ! colony morphology phenotypic evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007082 name: colony size phenotypic evidence used in manual assertion def: "A type of colony size phenotypic evidence that is used in an manual assertion." [ECO:RCT] synonym: "colony area phenotype evidence" NARROW [] synonym: "colony diameter phenotype evidence" NARROW [] synonym: "colony size evidence used in manual assertion" EXACT [] is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion is_a: ECO:0007081 ! colony size phenotypic evidence intersection_of: ECO:0007081 ! colony size phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007083 name: zone of inhibition evidence def: "A type of cell growth assay evidence resulting from assessing the sensitivity or resistance of bacteria or fungi to an antimicrobial agent or chemical by measuring the size of the zone of inhibition that results when the test organism is grown on a solid surface in the presence of the antimicrobial agent or chemical." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC153338/] is_a: ECO:0001563 ! cell growth assay evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007084 name: zone of inhibition evidence used in manual assertion def: "A type of zone of inhibition evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0007083 ! zone of inhibition evidence is_a: ECO:0007699 {is_inferred="true"} ! cell growth assay evidence used in manual assertion intersection_of: ECO:0007083 ! zone of inhibition evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007085 name: Etest evidence def: "A type of zone of inhibition evidence resulting from testing the antimicrobial susceptibility by using predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip." [URL:http\://www.joponline.org/doi/pdf/10.1902/jop.1992.63.7.576] synonym: "epsilometer test" EXACT [] is_a: ECO:0007083 ! zone of inhibition evidence property_value: IAO:0000234 "OMP" xsd:string created_by: snadendla creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007086 name: Etest evidence used in manual assertion def: "A type of Etest evidence that is used in a manual assertion." [ECO:RCT] synonym: "epsilometer test" BROAD [] is_a: ECO:0007084 {is_inferred="true"} ! zone of inhibition evidence used in manual assertion is_a: ECO:0007085 ! Etest evidence intersection_of: ECO:0007085 ! Etest evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string property_value: IAO:0000234 "OMP" xsd:string created_by: rctauber creation_date: 2018-03-14T12:44:11Z [Term] id: ECO:0007087 name: ribosome profiling evidence def: "A type of expression pattern evidence in which translation is measured by the positions of ribosomes active in a cell, identified through deep sequencing of ribosome-protected mRNA fragments." [PMID:27015305] synonym: "ribo-seq evidence" EXACT [] synonym: "ribosome footprinting evidence" EXACT [] synonym: "translation profiling" NARROW [] synonym: "translatome profiling" NARROW [] is_a: ECO:0000008 ! expression pattern evidence property_value: IAO:0000234 GOC:VW xsd:string created_by: rctauber creation_date: 2018-03-28T10:27:09Z [Term] id: ECO:0007088 name: ribosome profiling evidence used in manual assertion def: "A type of ribosome profiling evidence that is used in a manual assertion." [ECO:RCT] synonym: "ribo-seq evidence" BROAD [] synonym: "ribosome footprinting evidence" BROAD [] is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion is_a: ECO:0007087 ! ribosome profiling evidence intersection_of: ECO:0007087 ! ribosome profiling evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2018-03-28T10:33:26Z [Term] id: ECO:0007089 name: loss-of-function mutant phenotype evidence used in manual assertion def: "A type of loss-of-function mutant phenotype evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000016 {is_inferred="true"} ! loss-of-function mutant phenotype evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000016 ! loss-of-function mutant phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007090 name: structural similarity evidence used in manual assertion def: "A type of structural similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000027 {is_inferred="true"} ! structural similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000027 ! structural similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007092 name: gain-of-function mutant phenotypic evidence used in manual assertion def: "A type of gain-of-function mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "gain-of-function mutant phenotype evidence used in manual assertion" EXACT [] is_a: ECO:0000042 {is_inferred="true"} ! gain-of-function mutant phenotypic evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000042 ! gain-of-function mutant phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007093 name: voucher specimen phenotypic analysis evidence used in manual assertion def: "A type of voucher specimen phenotypic analysis evidence that is used in a manual assertion." [ECO:RCT] synonym: "voucher specimen analysis evidence used in manual assertion" EXACT [] is_a: ECO:0000050 {is_inferred="true"} ! voucher specimen phenotypic analysis evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000050 ! voucher specimen phenotypic analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007094 name: positional similarity evidence used in manual assertion def: "A type of positional similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000060 {is_inferred="true"} ! positional similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000060 ! positional similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007095 name: quantitative trait analysis evidence used in manual assertion def: "A type of quantitative trait analysis evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000061 {is_inferred="true"} ! quantitative trait analysis evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000061 ! quantitative trait analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007096 name: compositional similarity evidence used in manual assertion def: "A type of compositional similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000063 {is_inferred="true"} ! compositional similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000063 ! compositional similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007097 name: developmental similarity evidence used in manual assertion def: "A type of developmental similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000067 {is_inferred="true"} ! developmental similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000067 ! developmental similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007098 name: morphological similarity evidence used in manual assertion def: "A type of morphological similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000071 {is_inferred="true"} ! morphological similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000071 ! morphological similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007099 name: gene expression similarity evidence used in manual assertion def: "A type of gene expression similarity evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000075 {is_inferred="true"} ! gene expression similarity evidence is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion intersection_of: ECO:0000075 ! gene expression similarity evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007100 name: methylation-specific polymerase chain reaction evidence used in manual assertion def: "A type of methylation-specific polymerase chain reaction evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000077 {is_inferred="true"} ! methylation-specific polymerase chain reaction evidence is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion intersection_of: ECO:0000077 ! methylation-specific polymerase chain reaction evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007101 name: southern hybridization evidence used in manual assertion def: "A type of southern hybridization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000078 {is_inferred="true"} ! southern hybridization evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000078 ! southern hybridization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007102 name: intermethylated site amplification evidence used in manual assertion def: "A type of intermethylated site amplification evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000086 {is_inferred="true"} ! amplification of intermethylated sites evidence is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion intersection_of: ECO:0000086 ! amplification of intermethylated sites evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007103 name: epitope-tagged protein immunolocalization evidence used in manual assertion def: "A type of epitope-tagged protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000092 {is_inferred="true"} ! epitope-tagged protein immunolocalization evidence is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion intersection_of: ECO:0000092 ! epitope-tagged protein immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007104 name: co-fractionation evidence used in manual assertion def: "A type of co-fractionation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000102 {is_inferred="true"} ! co-fractionation evidence is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion intersection_of: ECO:0000102 ! co-fractionation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007106 name: green fluorescent protein fusion protein localization evidence used in manual assertion def: "A type of green fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000126 {is_inferred="true"} ! green fluorescent protein fusion protein localization evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000126 ! green fluorescent protein fusion protein localization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007107 name: yellow fluorescent protein fusion protein localization evidence used in manual assertion def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000128 {is_inferred="true"} ! yellow fluorescent protein fusion protein localization evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007108 name: beta-glucuronidase fusion protein localization evidence used in manual assertion def: "A type of beta-glucuronidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000130 {is_inferred="true"} ! beta-glucuronidase fusion protein localization evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007109 name: beta-galactosidase fusion protein localization evidence used in manual assertion def: "A type of beta-galactosidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000132 {is_inferred="true"} ! beta-galactosidase fusion protein localization evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000132 ! beta-galactosidase fusion protein localization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007110 name: thin layer chromatography evidence used in manual assertion def: "A type of thin layer chromatography evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000140 {is_inferred="true"} ! thin layer chromatography evidence is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion intersection_of: ECO:0000140 ! thin layer chromatography evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007111 name: in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000152 {is_inferred="true"} ! in vitro recombinant protein transcription reconstitution assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007112 name: protein separation followed by direct sequencing evidence used in manual assertion def: "A type of protein separation followed by direct sequencing evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000158 {is_inferred="true"} ! protein separation followed by direct sequencing evidence is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion intersection_of: ECO:0000158 ! protein separation followed by direct sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007113 name: protein separation followed by fragment identification evidence used in manual assertion def: "A type of protein separation followed by fragment identification evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000160 {is_inferred="true"} ! protein separation followed by fragment identification evidence is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion intersection_of: ECO:0000160 ! protein separation followed by fragment identification evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007114 name: heterologous system uptake evidence used in manual assertion def: "A type of heterologous system uptake evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000162 {is_inferred="true"} ! heterologous system uptake evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000162 ! heterologous system uptake evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007115 name: two-electrode voltage clamp recording evidence used in manual assertion def: "A type of two-electrode voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000166 {is_inferred="true"} ! two-electrode voltage clamp recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0000166 ! two-electrode voltage clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007116 name: biochemical trait analysis evidence used in manual assertion def: "A type of biochemical trait analysis evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000172 {is_inferred="true"} ! biochemical trait analysis evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000172 ! biochemical trait analysis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007117 name: mutant physiological response evidence used in manual assertion def: "A type of mutant physiological response evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000174 {is_inferred="true"} ! mutant physiological response evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000174 ! mutant physiological response evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007118 name: mutant visible phenotype evidence used in manual assertion def: "A type of mutant visible phenotype evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000176 {is_inferred="true"} ! mutant visible phenotype evidence is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion intersection_of: ECO:0000176 ! mutant visible phenotype evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007119 name: in vivo assay evidence used in manual assertion def: "A type of in vivo assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000178 {is_inferred="true"} ! in vivo assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000178 ! in vivo assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007120 name: animal model system study evidence used in manual assertion def: "A type of animal model system study evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000179 {is_inferred="true"} ! animal model system study evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000179 ! animal model system study evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007121 name: clinical study evidence used in manual assertion def: "A type of clinical study evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000180 {is_inferred="true"} ! clinical study evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000180 ! clinical study evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007122 name: in vitro assay evidence used in manual assertion def: "A type of in vitro assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000181 {is_inferred="true"} ! in vitro assay evidence is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion intersection_of: ECO:0000181 ! in vitro assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007123 name: enzyme inhibition evidence used in manual assertion def: "A type of enzyme inhibition evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000184 {is_inferred="true"} ! enzyme inhibition evidence is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion is_a: ECO:0007707 {is_inferred="true"} ! protein inhibition evidence used in manual assertion intersection_of: ECO:0000184 ! enzyme inhibition evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007125 name: Illumina sequencing evidence used in manual assertion def: "A type of Illumina sequencing evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000222 {is_inferred="true"} ! Illumina sequencing evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000222 ! Illumina sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007126 name: 454 pyrosequencing evidence used in manual assertion def: "A type of 454 pyrosequencing evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000223 {is_inferred="true"} ! 454 pyrosequencing evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000223 ! 454 pyrosequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007127 name: SOLiD sequencing evidence used in manual assertion def: "A type of SOLiD sequencing evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000224 {is_inferred="true"} ! SOLiD sequencing evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000224 ! SOLiD sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007128 name: chain termination sequencing evidence used in manual assertion def: "A type of chain termination sequencing evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000225 {is_inferred="true"} ! chain termination sequencing evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000225 ! chain termination sequencing evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007129 name: chromatin immunoprecipitation-qPCR evidence used in manual assertion def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000228 {is_inferred="true"} ! chromatin immunoprecipitation-qPCR evidence is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion intersection_of: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IPI" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007130 name: 4C evidence used in manual assertion def: "A type of 4C evidence that is used in a manual assertion." [ECO:RCT] synonym: "circularized chromosome conformation capture evidence used in manual assertion" EXACT [] is_a: ECO:0000234 {is_inferred="true"} ! 4C evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000234 ! 4C evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007131 name: 5C evidence used in manual assertion def: "A type of 5C evidence that is used in a manual assertion." [ECO:RCT] synonym: "carbon-copy chromosome conformation capture evidence used in manual assertion" EXACT [] is_a: ECO:0000235 {is_inferred="true"} ! 5C evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000235 ! 5C evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007133 name: 3C-qPCR evidence used in manual assertion def: "A type of 3C-qPCR evidence that is used in a manual assertion." [ECO:RCT] synonym: "chromosome conformation capture-qPCR evidence used in manual assertion" EXACT [] is_a: ECO:0000237 {is_inferred="true"} ! 3C-qPCR evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000237 ! 3C-qPCR evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007134 name: Hi-C evidence used in manual assertion def: "A type of Hi-C evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000238 {is_inferred="true"} ! Hi-C evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000238 ! Hi-C evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007135 name: 3C-seq evidence used in manual assertion def: "A type of 3C-seq evidence that is used in a manual assertion." [ECO:RCT] synonym: "chromosome conformation capture sequencing evidence used in manual assertion" EXACT [] is_a: ECO:0000239 {is_inferred="true"} ! 3C-seq evidence is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion intersection_of: ECO:0000239 ! 3C-seq evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007136 name: environmental perturbation phenotypic evidence used in manual assertion def: "A type of environmental perturbation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "environmental perturbation evidence used in manual assertion" EXACT [] is_a: ECO:0000241 {is_inferred="true"} ! environmental perturbation phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000241 ! environmental perturbation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007137 name: tissue ablation phenotypic evidence used in manual assertion def: "A type of tissue ablation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "tissue ablation evidence used in manual assertion" EXACT [] is_a: ECO:0000242 {is_inferred="true"} ! tissue ablation phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000242 ! tissue ablation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007138 name: tissue grafting phenotypic evidence used in manual assertion def: "A type of tissue grafting phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "tissue grafting evidence used in manual assertion" EXACT [] is_a: ECO:0000243 {is_inferred="true"} ! tissue grafting phenotypic evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000243 ! tissue grafting phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007141 name: cytochalasin experiment evidence used in manual assertion def: "A type of cytochalasin experiment evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000299 {is_inferred="true"} ! cytochalasin experiment evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0000299 ! cytochalasin experiment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007142 name: green fluorescent protein immunolocalization evidence used in manual assertion def: "A type of green fluorescent protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000300 {is_inferred="true"} ! green fluorescent protein immunolocalization evidence is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion intersection_of: ECO:0000300 ! green fluorescent protein immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007143 name: beta-galactosidase protein immunolocalization evidence used in manual assertion def: "A type of beta-galactosidase protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000301 {is_inferred="true"} ! beta-galactosidase protein immunolocalization evidence is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion intersection_of: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007144 name: cap analysis of gene expression evidence used in manual assertion def: "A type of cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000309 {is_inferred="true"} ! cap analysis of gene expression evidence intersection_of: ECO:0000309 ! cap analysis of gene expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007145 name: nano-cap analysis of gene expression evidence used in manual assertion def: "A type of nano-cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000310 {is_inferred="true"} ! nano-cap analysis of gene expression evidence intersection_of: ECO:0000310 ! nano-cap analysis of gene expression evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007146 name: particle size and count assay evidence used in manual assertion def: "A type of particle size and count assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000334 {is_inferred="true"} ! particle size and count assay evidence intersection_of: ECO:0000334 ! particle size and count assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007147 name: competitive growth assay evidence used in manual assertion def: "A type of competitive growth assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000336 ! competitive growth assay evidence is_a: ECO:0007696 {is_inferred="true"} ! cell proliferation assay evidence used in manual assertion intersection_of: ECO:0000336 ! competitive growth assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007148 name: pulsed-field gel electrophoresis evidence used in manual assertion def: "A type of pulsed-field gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000338 {is_inferred="true"} ! pulsed-field gel electrophoresis evidence intersection_of: ECO:0000338 ! pulsed-field gel electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007149 name: two-dimensional agarose gel electrophoresis evidence used in manual assertion def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0000339 {is_inferred="true"} ! two-dimensional agarose gel electrophoresis evidence intersection_of: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007150 name: plasmid maintenance assay evidence used in manual assertion def: "A type of plasmid maintenance assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0000340 {is_inferred="true"} ! plasmid maintenance assay evidence intersection_of: ECO:0000340 ! plasmid maintenance assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007151 name: specific protein inhibition by antibody evidence used in manual assertion def: "A type of specific protein inhibition by antibody evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000341 {is_inferred="true"} ! specific protein inhibition by antibody evidence is_a: ECO:0007707 {is_inferred="true"} ! protein inhibition evidence used in manual assertion intersection_of: ECO:0000341 ! specific protein inhibition by antibody evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007152 name: single exon transcript confirmation via alignment evidence used in manual assertion def: "A type of single exon transcript confirmation via alignment evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion is_a: ECO:0000345 {is_inferred="true"} ! single exon transcript confirmation via alignment evidence intersection_of: ECO:0000345 ! single exon transcript confirmation via alignment evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "ISA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007153 name: phylogenetic distribution evidence used in manual assertion def: "A type of phylogenetic distribution evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion is_a: ECO:0000355 {is_inferred="true"} ! phylogenetic distribution evidence intersection_of: ECO:0000355 ! phylogenetic distribution evidence intersection_of: used_in ECO:0000218 ! manual assertion created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007154 name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000358 {is_inferred="true"} ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) intersection_of: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007155 name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion is_a: ECO:0000359 {is_inferred="true"} ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) intersection_of: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IEP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007156 name: biological target-disease association via drug evidence used in manual assertion def: "A type of biological target-disease association via drug that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion is_a: ECO:0000360 {is_inferred="true"} ! biological target-disease association via drug evidence intersection_of: ECO:0000360 ! biological target-disease association via drug evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007157 name: cell staining evidence used in manual assertion def: "A type of cell staining evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001603 ! cell staining evidence is_a: ECO:0007695 {is_inferred="true"} ! cell-based assay evidence used in manual assertion intersection_of: ECO:0001603 ! cell staining evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007158 name: visual sequence inspection evidence used in manual assertion def: "A type of visual sequence inspection evidence that is used in a manual assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007159 name: ATP bioluminescence assay evidence used in manual assertion def: "A type of ATP bioluminescence assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005011 ! ATP bioluminescence assay evidence is_a: ECO:0007703 {is_inferred="true"} ! cell viability assay evidence used in manual assertion intersection_of: ECO:0005011 ! ATP bioluminescence assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007160 name: missense mutation pohenotypic evidence used in manual assertion def: "A type of missense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "missense mutation evidence used in manual assertion" EXACT [] is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion is_a: ECO:0005506 {is_inferred="true"} ! missense mutation phenotypic evidence intersection_of: ECO:0005506 ! missense mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007161 name: nonsense mutation phenotypic evidence used in manual assertion def: "A type of nonsense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "nonsense mutation evidence used in manual assertion" EXACT [] is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion is_a: ECO:0005507 {is_inferred="true"} ! nonsense mutation phenotypic evidence intersection_of: ECO:0005507 ! nonsense mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007162 name: silent mutation evidence used in manual assertion def: "A type of silent mutation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion is_a: ECO:0005508 {is_inferred="true"} ! silent mutation evidence intersection_of: ECO:0005508 ! silent mutation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007163 name: insertion mutation phenotypic evidence used in manual assertion def: "A type of insertion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "insertion mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005509 {is_inferred="true"} ! insertion mutation phenotypic evidence intersection_of: ECO:0005509 ! insertion mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007164 name: duplication mutation evidence used in manual assertion def: "A type of duplication mutation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005511 {is_inferred="true"} ! duplication mutation evidence is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion intersection_of: ECO:0005511 ! duplication mutation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007165 name: frameshift mutation phenotypic evidence used in manual assertion def: "A type of frameshift mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "frameshift mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005512 {is_inferred="true"} ! frameshift mutation phenotypic evidence intersection_of: ECO:0005512 ! frameshift mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007166 name: repeat expansion mutation phenotypic evidence used in manual assertion def: "A type of repeat expansion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "repeat expansion mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005513 {is_inferred="true"} ! repeat expansion mutation phenotypic evidence intersection_of: ECO:0005513 ! repeat expansion mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007167 name: splice site mutation phenotypic evidence used in manual assertion def: "A type of splice site mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "splice site mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005514 {is_inferred="true"} ! splice site mutation phenotypic evidence intersection_of: ECO:0005514 ! splice site mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007168 name: translocation mutation phenotypic evidence used in manual assertion def: "A type of translocation mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] synonym: "translocation mutation evidence used in manual assertion" EXACT [] is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion is_a: ECO:0005515 {is_inferred="true"} ! translocation mutation phenotypic evidence intersection_of: ECO:0005515 ! translocation mutation phenotypic evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IMP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007169 name: in-gel protein kinase assay evidence used in manual assertion def: "A type of in-gel protein kinase assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005562 {is_inferred="true"} ! in-gel protein kinase assay evidence is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion intersection_of: ECO:0005562 ! in-gel protein kinase assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007170 name: macroscopic current trace evidence used in manual assertion def: "A type of macroscopic current trace evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005563 {is_inferred="true"} ! macroscopic current trace evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005563 ! macroscopic current trace evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007171 name: current density evidence used in manual assertion def: "A type of current density evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005564 {is_inferred="true"} ! current density evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005564 ! current density evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007172 name: sustained current evidence used in manual assertion def: "A type of sustained current evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005566 {is_inferred="true"} ! sustained current evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005566 ! sustained current evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007173 name: use dependence of inactivation evidence used in manual assertion def: "A type of use dependence of inactivation evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005570 {is_inferred="true"} ! use dependence of inactivation evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005570 ! use dependence of inactivation evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007174 name: current clamp recording evidence used in manual assertion def: "A type of current clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005571 {is_inferred="true"} ! current clamp recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005571 ! current clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007175 name: whole-cell voltage clamp recording evidence used in manual assertion def: "A type of whole-cell voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005572 {is_inferred="true"} ! whole-cell voltage clamp recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005572 ! whole-cell voltage clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007176 name: cell-attached single-channel recording evidence used in manual assertion def: "A type of cell-attached single-channel recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005573 {is_inferred="true"} ! cell-attached single-channel recording evidence is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion intersection_of: ECO:0005573 ! cell-attached single-channel recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007177 name: cell-detached inside-out single-channel recording evidence used in manual assertion def: "A type of cell-detached inside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005574 {is_inferred="true"} ! cell-detached inside-out single-channel recording evidence is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion intersection_of: ECO:0005574 ! cell-detached inside-out single-channel recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007178 name: reconstituted bilayer single-channel patch recording evidence used in manual assertion def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005575 {is_inferred="true"} ! reconstituted bilayer single-channel patch recording evidence is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion intersection_of: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007179 name: electroencephalography recording evidence used in manual assertion def: "A type of electroencephalography recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005577 {is_inferred="true"} ! electroencephalography recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005577 ! electroencephalography recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007180 name: cell-detached outside-out single-channel recording evidence used in manual assertion def: "A type of cell-detached outside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005582 {is_inferred="true"} ! cell-detached outside-out single-channel recording evidence is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion intersection_of: ECO:0005582 ! cell-detached outside-out single-channel recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007181 name: cut-open oocyte voltage clamp recording evidence used in manual assertion def: "A type of cut-open oocyte voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005583 {is_inferred="true"} ! cut-open oocyte voltage clamp recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007182 name: macropatch voltage clamp recording evidence used in manual assertion def: "A type of macropatch voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005584 {is_inferred="true"} ! macropatch voltage clamp recording evidence is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion intersection_of: ECO:0005584 ! macropatch voltage clamp recording evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007184 name: protein mass spectrometry evidence used in manual assertion def: "A type of protein mass spectrometry evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion is_a: ECO:0005603 {is_inferred="true"} ! protein mass spectrometry evidence intersection_of: ECO:0005603 ! protein mass spectrometry evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007185 name: cross-streak test evidence used in manual assertion def: "A type of cross-streak test evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005604 ! cross-streak test evidence is_a: ECO:0007696 {is_inferred="true"} ! cell proliferation assay evidence used in manual assertion intersection_of: ECO:0005604 ! cross-streak test evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007186 name: tethered cell assay evidence used in manual assertion def: "A type of tethered cell assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005607 ! tethered cell assay evidence is_a: ECO:0007701 {is_inferred="true"} ! chemotaxis assay evidence used in manual assertion intersection_of: ECO:0005607 ! tethered cell assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007187 name: tumble frequency assay evidence used in manual assertion def: "A type of tumble frequency assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005608 ! tumble frequency assay evidence is_a: ECO:0007701 {is_inferred="true"} ! chemotaxis assay evidence used in manual assertion intersection_of: ECO:0005608 ! tumble frequency assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007188 name: capillary assay evidence used in manual assertion def: "A type of capillary assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0005609 ! capillary assay evidence is_a: ECO:0007701 {is_inferred="true"} ! chemotaxis assay evidence used in manual assertion intersection_of: ECO:0005609 ! capillary assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007189 name: inference from experimental data evidence used in manual assertion def: "A type of inference from experimental data evidence that is used in a manual assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007190 name: inference from phenotype manipulation evidence used in manual assertion def: "A type of inference from phenotype manipulation evidence that is used in a manual assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007191 name: inference by association of genotype from phenotype used in manual assertion def: "A type of inference by association of genotype from phenotype that is used in a manual assertion." [ECO:RCT] is_a: ECO:0001237 {is_inferred="true"} ! natural variation mutant evidence used in manual assertion intersection_of: ECO:0005613 ! inference by association of genotype from phenotype intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "EXP" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007192 name: motility assay evidence used in manual assertion def: "A type of motility assay evidence that is used in a manual assertion." [ECO:RCT] is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion is_a: ECO:0005803 {is_inferred="true"} ! motility assay evidence intersection_of: ECO:0005803 ! motility assay evidence intersection_of: used_in ECO:0000218 ! manual assertion property_value: ECO:9000002 "IDA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007193 name: loss-of-function mutant phenotype evidence used in automatic assertion def: "A type of loss-of-function mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000016 {is_inferred="true"} ! loss-of-function mutant phenotype evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0000016 ! loss-of-function mutant phenotype evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007194 name: structural similarity evidence used in automatic assertion def: "A type of structural similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000027 {is_inferred="true"} ! structural similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000027 ! structural similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007196 name: gain-of-function mutant phenotypic evidence used in automatic assertion def: "A type of gain-of-function mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "gain-of-function mutant phenotype evidence used in automatic assertion" EXACT [] is_a: ECO:0000042 {is_inferred="true"} ! gain-of-function mutant phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0000042 ! gain-of-function mutant phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007197 name: voucher specimen phenotypic analysis evidence used in automatic assertion def: "A type of voucher specimen phenotypic analysis evidence that is used in an automatic assertion." [ECO:RCT] synonym: "voucher specimen analysis evidence used in automatic assertion" EXACT [] is_a: ECO:0000050 {is_inferred="true"} ! voucher specimen phenotypic analysis evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000050 ! voucher specimen phenotypic analysis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007198 name: positional similarity evidence used in automatic assertion def: "A type of positional similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000060 {is_inferred="true"} ! positional similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000060 ! positional similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007199 name: quantitative trait analysis evidence used in automatic assertion def: "A type of quantitative trait analysis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000061 {is_inferred="true"} ! quantitative trait analysis evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000061 ! quantitative trait analysis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007200 name: compositional similarity evidence used in automatic assertion def: "A type of compositional similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000063 {is_inferred="true"} ! compositional similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000063 ! compositional similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007201 name: developmental similarity evidence used in automatic assertion def: "A type of developmental similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000067 {is_inferred="true"} ! developmental similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000067 ! developmental similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007202 name: morphological similarity evidence used in automatic assertion def: "A type of morphological similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000071 {is_inferred="true"} ! morphological similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000071 ! morphological similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007203 name: gene expression similarity evidence used in automatic assertion def: "A type of gene expression similarity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000075 {is_inferred="true"} ! gene expression similarity evidence is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion intersection_of: ECO:0000075 ! gene expression similarity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007204 name: methylation-specific polymerase chain reaction evidence used in automatic assertion def: "A type of methylation-specific polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000077 {is_inferred="true"} ! methylation-specific polymerase chain reaction evidence is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion intersection_of: ECO:0000077 ! methylation-specific polymerase chain reaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007205 name: southern hybridization evidence used in automatic assertion def: "A type of southern hybridization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000078 {is_inferred="true"} ! southern hybridization evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0000078 ! southern hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007206 name: intermethylated site amplification evidence used in automatic assertion def: "A type of intermethylated site amplification evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000086 {is_inferred="true"} ! amplification of intermethylated sites evidence is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion intersection_of: ECO:0000086 ! amplification of intermethylated sites evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007207 name: epitope-tagged protein immunolocalization evidence used in automatic assertion def: "A type of epitope-tagged protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000092 {is_inferred="true"} ! epitope-tagged protein immunolocalization evidence is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion intersection_of: ECO:0000092 ! epitope-tagged protein immunolocalization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007208 name: co-fractionation evidence used in automatic assertion def: "A type of co-fractionation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000102 {is_inferred="true"} ! co-fractionation evidence is_a: ECO:0007534 {is_inferred="true"} ! fractionation evidence used in automatic assertion intersection_of: ECO:0000102 ! co-fractionation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007210 name: green fluorescent protein fusion protein localization evidence used in automatic assertion def: "A type of green fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000126 {is_inferred="true"} ! green fluorescent protein fusion protein localization evidence is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion intersection_of: ECO:0000126 ! green fluorescent protein fusion protein localization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007211 name: yellow fluorescent protein fusion protein localization evidence used in automatic assertion def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000128 {is_inferred="true"} ! yellow fluorescent protein fusion protein localization evidence is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion intersection_of: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007212 name: beta-glucuronidase fusion protein localization evidence used in automatic assertion def: "A type of beta-glucuronidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000130 {is_inferred="true"} ! beta-glucuronidase fusion protein localization evidence is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion intersection_of: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007213 name: beta-galactosidase fusion protein localization evidence used in automatic assertion def: "A type of beta-galactosidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000132 {is_inferred="true"} ! beta-galactosidase fusion protein localization evidence is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion intersection_of: ECO:0000132 ! beta-galactosidase fusion protein localization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007214 name: thin layer chromatography evidence used in automatic assertion def: "A type of thin layer chromatography evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000140 {is_inferred="true"} ! thin layer chromatography evidence is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion intersection_of: ECO:0000140 ! thin layer chromatography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007215 name: in vitro recombinant protein transcription reconstitution assay evidence used in automatic assertion def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000152 {is_inferred="true"} ! in vitro recombinant protein transcription reconstitution assay evidence is_a: ECO:0007804 {is_inferred="true"} ! in vitro transcription reconstitution assay evidence used in automatic assertion intersection_of: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007216 name: protein separation followed by direct sequencing evidence used in automatic assertion def: "A type of protein separation followed by direct sequencing evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000158 {is_inferred="true"} ! protein separation followed by direct sequencing evidence is_a: ECO:0007787 {is_inferred="true"} ! protein separation evidence used in automatic assertion intersection_of: ECO:0000158 ! protein separation followed by direct sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007217 name: protein separation followed by fragment identification evidence used in automatic assertion def: "A type of protein separation followed by fragment identification evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000160 {is_inferred="true"} ! protein separation followed by fragment identification evidence is_a: ECO:0007787 {is_inferred="true"} ! protein separation evidence used in automatic assertion intersection_of: ECO:0000160 ! protein separation followed by fragment identification evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007218 name: heterologous system uptake evidence used in automatic assertion def: "A type of heterologous system uptake evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000162 {is_inferred="true"} ! heterologous system uptake evidence is_a: ECO:0007789 {is_inferred="true"} ! transport assay evidence used in automatic assertion intersection_of: ECO:0000162 ! heterologous system uptake evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007219 name: two-electrode voltage clamp recording evidence used in automatic assertion def: "A type of two-electrode voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000166 {is_inferred="true"} ! two-electrode voltage clamp recording evidence is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion intersection_of: ECO:0000166 ! two-electrode voltage clamp recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007220 name: biochemical trait analysis evidence used in automatic assertion def: "A type of biochemical trait analysis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000172 {is_inferred="true"} ! biochemical trait analysis evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000172 ! biochemical trait analysis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007221 name: mutant physiological response evidence used in automatic assertion def: "A type of mutant physiological response evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000174 {is_inferred="true"} ! mutant physiological response evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0000174 ! mutant physiological response evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007222 name: mutant visible phenotype evidence used in automatic assertion def: "A type of mutant visible phenotype evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000176 {is_inferred="true"} ! mutant visible phenotype evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0000176 ! mutant visible phenotype evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007223 name: in vivo assay evidence used in automatic assertion def: "A type of in vivo assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000178 {is_inferred="true"} ! in vivo assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000178 ! in vivo assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007224 name: animal model system study evidence used in automatic assertion def: "A type of animal model system study evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000179 {is_inferred="true"} ! animal model system study evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000179 ! animal model system study evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007225 name: clinical study evidence used in automatic assertion def: "A type of clinical study evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000180 {is_inferred="true"} ! clinical study evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000180 ! clinical study evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007226 name: in vitro assay evidence used in automatic assertion def: "A type of in vitro assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000181 {is_inferred="true"} ! in vitro assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000181 ! in vitro assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007227 name: enzyme inhibition evidence used in automatic assertion def: "A type of enzyme inhibition evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000184 {is_inferred="true"} ! enzyme inhibition evidence is_a: ECO:0007468 {is_inferred="true"} ! enzymatic activity assay evidence used in automatic assertion is_a: ECO:0007785 {is_inferred="true"} ! protein inhibition evidence used in automatic assertion intersection_of: ECO:0000184 ! enzyme inhibition evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007229 name: Illumina sequencing evidence used in automatic assertion def: "A type of Illumina sequencing evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000222 {is_inferred="true"} ! Illumina sequencing evidence is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0000222 ! Illumina sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007230 name: 454 pyrosequencing evidence used in automatic assertion def: "A type of 454 pyrosequencing evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000223 {is_inferred="true"} ! 454 pyrosequencing evidence is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0000223 ! 454 pyrosequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007231 name: SOLiD sequencing evidence used in automatic assertion def: "A type of SOLiD sequencing evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000224 {is_inferred="true"} ! SOLiD sequencing evidence is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0000224 ! SOLiD sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007232 name: chain termination sequencing evidence used in automatic assertion def: "A type of chain termination sequencing evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000225 {is_inferred="true"} ! chain termination sequencing evidence is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0000225 ! chain termination sequencing evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007233 name: chromatin immunoprecipitation-qPCR evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000228 {is_inferred="true"} ! chromatin immunoprecipitation-qPCR evidence is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion intersection_of: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007234 name: 4C evidence used in automatic assertion def: "A type of 4C evidence that is used in an automatic assertion." [ECO:RCT] synonym: "circularized chromosome conformation capture evidence used in automatic assertion" EXACT [] is_a: ECO:0000234 {is_inferred="true"} ! 4C evidence is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion intersection_of: ECO:0000234 ! 4C evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007235 name: 5C evidence used in automatic assertion def: "A type of 5C evidence that is used in an automatic assertion." [ECO:RCT] synonym: "carbon-copy chromosome conformation capture evidence used in automatic assertion" EXACT [] is_a: ECO:0000235 {is_inferred="true"} ! 5C evidence is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion intersection_of: ECO:0000235 ! 5C evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007237 name: 3C-qPCR evidence used in automatic assertion def: "A type of 3C-qPCR evidence that is used in an automatic assertion." [ECO:RCT] synonym: "chromosome conformation capture-qPCR evidence used in automatic assertion" EXACT [] is_a: ECO:0000237 {is_inferred="true"} ! 3C-qPCR evidence is_a: ECO:0007823 {is_inferred="true"} ! 3C evidence used in automatic assertion intersection_of: ECO:0000237 ! 3C-qPCR evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007238 name: Hi-C evidence used in automatic assertion def: "A type of Hi-C evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000238 {is_inferred="true"} ! Hi-C evidence is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion intersection_of: ECO:0000238 ! Hi-C evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007239 name: 3C-seq evidence used in automatic assertion def: "A type of 3C-seq evidence that is used in an automatic assertion." [ECO:RCT] synonym: "chromosome conformation capture sequencing evidence used in automatic assertion" EXACT [] is_a: ECO:0000239 {is_inferred="true"} ! 3C-seq evidence is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion intersection_of: ECO:0000239 ! 3C-seq evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007240 name: environmental perturbation phenotypic evidence used in automatic assertion def: "A type of environmental perturbation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "environmental perturbation evidence used in automatic assertion" EXACT [] is_a: ECO:0000241 {is_inferred="true"} ! environmental perturbation phenotypic evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000241 ! environmental perturbation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007241 name: tissue ablation phenotypic evidence used in automatic assertion def: "A type of tissue ablation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "tissue ablation evidence used in automatic assertion" EXACT [] is_a: ECO:0000242 {is_inferred="true"} ! tissue ablation phenotypic evidence is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion intersection_of: ECO:0000242 ! tissue ablation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007242 name: tissue grafting phenotypic evidence used in automatic assertion def: "A type of tissue grafting phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "tissue grafting evidence used in automatic assertion" EXACT [] is_a: ECO:0000243 {is_inferred="true"} ! tissue grafting phenotypic evidence is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion intersection_of: ECO:0000243 ! tissue grafting phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007245 name: cytochalasin experiment evidence used in automatic assertion def: "A type of cytochalasin experiment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000299 {is_inferred="true"} ! cytochalasin experiment evidence is_a: ECO:0007811 {is_inferred="true"} ! cleavage arrested development evidence used in automatic assertion intersection_of: ECO:0000299 ! cytochalasin experiment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007246 name: green fluorescent protein immunolocalization evidence used in automatic assertion def: "A type of green fluorescent protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000300 {is_inferred="true"} ! green fluorescent protein immunolocalization evidence is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion intersection_of: ECO:0000300 ! green fluorescent protein immunolocalization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007247 name: beta-galactosidase protein immunolocalization evidence used in automatic assertion def: "A type of beta-galactosidase protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000301 {is_inferred="true"} ! beta-galactosidase protein immunolocalization evidence is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion intersection_of: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007248 name: cap analysis of gene expression evidence used in automatic assertion def: "A type of cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000309 {is_inferred="true"} ! cap analysis of gene expression evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000309 ! cap analysis of gene expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007249 name: nano-cap analysis of gene expression evidence used in automatic assertion def: "A type of nano-cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000310 {is_inferred="true"} ! nano-cap analysis of gene expression evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000310 ! nano-cap analysis of gene expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007250 name: particle size and count assay evidence used in automatic assertion def: "A type of particle size and count assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000334 {is_inferred="true"} ! particle size and count assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000334 ! particle size and count assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007251 name: competitive growth assay evidence used in automatic assertion def: "A type of competitive growth assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000336 {is_inferred="true"} ! competitive growth assay evidence is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0000336 ! competitive growth assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007252 name: pulsed-field gel electrophoresis evidence used in automatic assertion def: "A type of pulsed-field gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000338 {is_inferred="true"} ! pulsed-field gel electrophoresis evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0000338 ! pulsed-field gel electrophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007253 name: two-dimensional agarose gel electrophoresis evidence used in automatic assertion def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000339 {is_inferred="true"} ! two-dimensional agarose gel electrophoresis evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007254 name: plasmid maintenance assay evidence used in automatic assertion def: "A type of plasmid maintenance assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000340 {is_inferred="true"} ! plasmid maintenance assay evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000340 ! plasmid maintenance assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007255 name: specific protein inhibition by antibody evidence used in automatic assertion def: "A type of specific protein inhibition by antibody evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000341 {is_inferred="true"} ! specific protein inhibition by antibody evidence is_a: ECO:0007785 {is_inferred="true"} ! protein inhibition evidence used in automatic assertion intersection_of: ECO:0000341 ! specific protein inhibition by antibody evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007256 name: single exon transcript confirmation via alignment evidence used in automatic assertion def: "A type of single exon transcript confirmation via alignment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion is_a: ECO:0000345 {is_inferred="true"} ! single exon transcript confirmation via alignment evidence intersection_of: ECO:0000345 ! single exon transcript confirmation via alignment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007257 name: phylogenetic distribution evidence used in automatic assertion def: "A type of phylogenetic distribution evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000355 {is_inferred="true"} ! phylogenetic distribution evidence is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion intersection_of: ECO:0000355 ! phylogenetic distribution evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007258 name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in automatic assertion def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000358 {is_inferred="true"} ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007259 name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in automatic assertion def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000359 {is_inferred="true"} ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007260 name: biological target-disease association via drug evidence used in automatic assertion def: "A type of biological target-disease association via drug that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000360 {is_inferred="true"} ! biological target-disease association via drug evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000360 ! biological target-disease association via drug evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007261 name: cell staining evidence used in automatic assertion def: "A type of cell staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001603 {is_inferred="true"} ! cell staining evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001603 ! cell staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007262 name: visual sequence inspection evidence used in automatic assertion def: "A type of visual sequence inspection evidence that is used in an automatic assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007263 name: ATP bioluminescence assay evidence used in automatic assertion def: "A type of ATP bioluminescence assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005011 {is_inferred="true"} ! ATP bioluminescence assay evidence is_a: ECO:0007774 {is_inferred="true"} ! cell viability assay evidence used in automatic assertion intersection_of: ECO:0005011 ! ATP bioluminescence assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007264 name: missense mutation phenotypic evidence used in automatic assertion def: "A type of missense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "missense mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005506 {is_inferred="true"} ! missense mutation phenotypic evidence is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion intersection_of: ECO:0005506 ! missense mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007265 name: nonsense mutation phenotypic evidence used in automatic assertion def: "A type of nonsense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "nonsense mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005507 {is_inferred="true"} ! nonsense mutation phenotypic evidence is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion intersection_of: ECO:0005507 ! nonsense mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007266 name: silent mutation evidence used in automatic assertion def: "A type of silent mutation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005508 {is_inferred="true"} ! silent mutation evidence is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion intersection_of: ECO:0005508 ! silent mutation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007267 name: insertion mutation phenotypic evidence used in automatic assertion def: "A type of insertion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "insertion mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005509 {is_inferred="true"} ! insertion mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005509 ! insertion mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007268 name: duplication mutation evidence used in automatic assertion def: "A type of duplication mutation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005511 {is_inferred="true"} ! duplication mutation evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0005511 ! duplication mutation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007269 name: frameshift mutation phenotypic evidence used in automatic assertion def: "A type of frameshift mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "frameshift mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005512 {is_inferred="true"} ! frameshift mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005512 ! frameshift mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007270 name: repeat expansion mutation phenotypic evidence used in automatic assertion def: "A type of repeat expansion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "repeat expansion mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005513 {is_inferred="true"} ! repeat expansion mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005513 ! repeat expansion mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007271 name: splice site mutation phenotypic evidence used in automatic assertion def: "A type of splice site mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "splice site mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005514 {is_inferred="true"} ! splice site mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005514 ! splice site mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007272 name: translocation mutation phenotypic evidence used in automatic assertion def: "A type of translocation mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "translocation mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0005515 {is_inferred="true"} ! translocation mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005515 ! translocation mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007273 name: in-gel protein kinase assay evidence used in automatic assertion def: "A type of in-gel protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005562 {is_inferred="true"} ! in-gel protein kinase assay evidence is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion intersection_of: ECO:0005562 ! in-gel protein kinase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007274 name: macroscopic current trace evidence used in automatic assertion def: "A type of macroscopic current trace evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005563 {is_inferred="true"} ! macroscopic current trace evidence is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion intersection_of: ECO:0005563 ! macroscopic current trace evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007275 name: current density evidence used in automatic assertion def: "A type of current density evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005564 {is_inferred="true"} ! current density evidence is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion intersection_of: ECO:0005564 ! current density evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007276 name: sustained current evidence used in automatic assertion def: "A type of sustained current evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005566 {is_inferred="true"} ! sustained current evidence is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion intersection_of: ECO:0005566 ! sustained current evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007277 name: use dependence of inactivation evidence used in automatic assertion def: "A type of use dependence of inactivation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005570 {is_inferred="true"} ! use dependence of inactivation evidence is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion intersection_of: ECO:0005570 ! use dependence of inactivation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007278 name: current clamp recording evidence used in automatic assertion def: "A type of current clamp recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005571 {is_inferred="true"} ! current clamp recording evidence is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion intersection_of: ECO:0005571 ! current clamp recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007279 name: whole-cell voltage clamp recording evidence used in automatic assertion def: "A type of whole-cell voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005572 {is_inferred="true"} ! whole-cell voltage clamp recording evidence is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion intersection_of: ECO:0005572 ! whole-cell voltage clamp recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007280 name: cell-attached single-channel recording evidence used in automatic assertion def: "A type of cell-attached single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005573 {is_inferred="true"} ! cell-attached single-channel recording evidence is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion intersection_of: ECO:0005573 ! cell-attached single-channel recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007281 name: cell-detached inside-out single-channel recording evidence used in automatic assertion def: "A type of cell-detached inside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005574 {is_inferred="true"} ! cell-detached inside-out single-channel recording evidence is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion intersection_of: ECO:0005574 ! cell-detached inside-out single-channel recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007282 name: reconstituted bilayer single-channel patch recording evidence used in automatic assertion def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005575 {is_inferred="true"} ! reconstituted bilayer single-channel patch recording evidence is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion intersection_of: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007283 name: electroencephalography recording evidence used in automatic assertion def: "A type of electroencephalography recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005577 {is_inferred="true"} ! electroencephalography recording evidence is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion intersection_of: ECO:0005577 ! electroencephalography recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007284 name: cell-detached outside-out single-channel recording evidence used in automatic assertion def: "A type of cell-detached outside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005582 {is_inferred="true"} ! cell-detached outside-out single-channel recording evidence is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion intersection_of: ECO:0005582 ! cell-detached outside-out single-channel recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007285 name: cut-open oocyte voltage clamp recording evidence used in automatic assertion def: "A type of cut-open oocyte voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005583 {is_inferred="true"} ! cut-open oocyte voltage clamp recording evidence is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion intersection_of: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007286 name: macropatch voltage clamp recording evidence used in automatic assertion def: "A type of macropatch voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005584 {is_inferred="true"} ! macropatch voltage clamp recording evidence is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion intersection_of: ECO:0005584 ! macropatch voltage clamp recording evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007288 name: protein mass spectrometry evidence used in automatic assertion def: "A type of protein mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005603 {is_inferred="true"} ! protein mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0005603 ! protein mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007289 name: cross-streak test evidence used in automatic assertion def: "A type of cross-streak test evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005604 {is_inferred="true"} ! cross-streak test evidence is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0005604 ! cross-streak test evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007290 name: tethered cell assay evidence used in automatic assertion def: "A type of tethered cell assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005607 {is_inferred="true"} ! tethered cell assay evidence is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion intersection_of: ECO:0005607 ! tethered cell assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007291 name: tumble frequency assay evidence used in automatic assertion def: "A type of tumble frequency assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005608 {is_inferred="true"} ! tumble frequency assay evidence is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion intersection_of: ECO:0005608 ! tumble frequency assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007292 name: capillary assay evidence used in automatic assertion def: "A type of capillary assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005609 {is_inferred="true"} ! capillary assay evidence is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion intersection_of: ECO:0005609 ! capillary assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007293 name: inference from experimental data evidence used in automatic assertion def: "A type of inference from experimental data evidence that is used in an automatic assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007294 name: inference from phenotype manipulation evidence used in automatic assertion def: "A type of inference from phenotype manipulation evidence that is used in an automatic assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007295 name: inference by association of genotype from phenotype used in automatic assertion def: "A type of inference by association of genotype from phenotype that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005613 {is_inferred="true"} ! inference by association of genotype from phenotype is_a: ECO:0007191 {is_inferred="true"} ! inference by association of genotype from phenotype used in manual assertion is_a: ECO:0007440 {is_inferred="true"} ! natural variation mutant evidence used in automatic assertion intersection_of: ECO:0005613 ! inference by association of genotype from phenotype intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "EXP" xsd:string property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007296 name: motility assay evidence used in automatic assertion def: "A type of motility assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005803 {is_inferred="true"} ! motility assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0005803 ! motility assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007297 name: experimental evidence used in automatic assertion def: "A type of experimental evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000006 ! experimental evidence is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion intersection_of: ECO:0000006 ! experimental evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007298 name: expression pattern evidence used in automatic assertion def: "A type of expression pattern evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000008 ! expression pattern evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000008 ! expression pattern evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007299 name: Affymetrix GeneChip evidence used in automatic assertion def: "A type of Affymetrix GeneChip evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000101 {is_inferred="true"} ! Affymetrix GeneChip evidence is_a: ECO:0007300 {is_inferred="true"} ! cRNA to DNA expression microarray evidence used in automatic assertion intersection_of: ECO:0000101 ! Affymetrix GeneChip evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007300 name: cRNA to DNA expression microarray evidence used in automatic assertion def: "A type of cRNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000062 {is_inferred="true"} ! cRNA to DNA expression microarray evidence is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion intersection_of: ECO:0000062 ! cRNA to DNA expression microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007301 name: expression microarray evidence used in automatic assertion def: "A type of expression microarray evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000058 {is_inferred="true"} ! expression microarray evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000058 ! expression microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007302 name: differential methylation hybridization evidence used in automatic assertion def: "A type of differential methylation hybridization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000069 {is_inferred="true"} ! differential methylation hybridization evidence is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion intersection_of: ECO:0000069 ! differential methylation hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007303 name: transcript expression evidence used in automatic assertion def: "A type of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] synonym: "transcriptomics evidence used in automatic assertion" EXACT [] is_a: ECO:0000009 {is_inferred="true"} ! transcript expression evidence is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion intersection_of: ECO:0000009 ! transcript expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007304 name: Nimblegen array evidence used in automatic assertion def: "A type of Nimblegen array evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000105 {is_inferred="true"} ! Nimblegen array evidence is_a: ECO:0005630 {is_inferred="true"} ! cDNA to DNA expression microarray evidence used in automatic assertion intersection_of: ECO:0000105 ! Nimblegen array evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007305 name: array-based sequence capture evidence used in automatic assertion def: "A type of array-based sequence capture evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000093 {is_inferred="true"} ! array-based sequence capture evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0000093 ! array-based sequence capture evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007306 name: qualitative western immunoblotting evidence used in automatic assertion alt_id: ECO:0007287 def: "A type of qualitative western immunoblotting evidence that is used in an automatic assertion." [ECO:RCT] synonym: "protein expression level evidence based on western blot used in automatic assertion" EXACT [] is_a: ECO:0000112 {is_inferred="true"} ! qualitative western immunoblotting evidence is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion is_a: ECO:0007770 {is_inferred="true"} ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion intersection_of: ECO:0000112 ! qualitative western immunoblotting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007307 name: direct assay evidence used in automatic assertion def: "A type of direct assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000002 {is_inferred="true"} ! direct assay evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0000002 ! direct assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007309 name: protein expression evidence used in automatic assertion alt_id: ECO:0007308 def: "A type of protein expression evidence that is used in an automatic assertion." [ECO:RCT] synonym: "protein expression level evidence used in automatic assertion" EXACT [] is_a: ECO:0000010 {is_inferred="true"} ! protein expression evidence is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion intersection_of: ECO:0000010 ! protein expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007310 name: expression library screen evidence used in automatic assertion def: "A type of expression library screen evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000114 {is_inferred="true"} ! expression library screen evidence is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion intersection_of: ECO:0000114 ! expression library screen evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007311 name: heterologous protein expression evidence used in automatic assertion def: "A type of heterologous protein expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000154 {is_inferred="true"} ! heterologous protein expression evidence is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion intersection_of: ECO:0000154 ! heterologous protein expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007312 name: spatial pattern of protein expression evidence used in automatic assertion def: "A type of spatial pattern of protein expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000045 {is_inferred="true"} ! spatial pattern of protein expression evidence is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion intersection_of: ECO:0000045 ! spatial pattern of protein expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007313 name: DNA to cDNA expression microarray evidence used in automatic assertion def: "A type of DNA to cDNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000104 {is_inferred="true"} ! DNA to cDNA expression microarray evidence is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion intersection_of: ECO:0000104 ! DNA to cDNA expression microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007314 name: differential hybridization evidence used in automatic assertion def: "A type of differential hybridization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000116 {is_inferred="true"} ! differential hybridization evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000116 ! differential hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007315 name: RNA protection assay evidence used in automatic assertion def: "A type of RNA protection assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000110 {is_inferred="true"} ! RNA protection assay evidence is_a: ECO:0007316 {is_inferred="true"} ! nuclease protection assay evidence used in automatic assertion intersection_of: ECO:0000110 ! RNA protection assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007316 name: nuclease protection assay evidence used in automatic assertion def: "A type of nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001106 {is_inferred="true"} ! nuclease protection assay evidence is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion intersection_of: ECO:0001106 ! nuclease protection assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007317 name: spatial pattern of transcript expression evidence used in automatic assertion def: "A type of spatial pattern of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000047 {is_inferred="true"} ! spatial pattern of transcript expression evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000047 ! spatial pattern of transcript expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007318 name: subtractive hybridization evidence used in automatic assertion def: "A type of subtractive hybridization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000118 {is_inferred="true"} ! subtractive hybridization evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000118 ! subtractive hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007319 name: author statement used in automatic assertion def: "A type of author statement that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000204 {is_inferred="true"} ! author statement is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion intersection_of: ECO:0000204 ! author statement intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007320 name: author statement without traceable support used in automatic assertion def: "A type of author statement without traceable support that is used in an automatic assertion." [ECO:RCT] synonym: "non-traceable author statement used in automatic assertion" RELATED [] is_a: ECO:0000034 {is_inferred="true"} ! author statement without traceable support is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion intersection_of: ECO:0000034 ! author statement without traceable support intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007321 name: author statement supported by traceable reference used in automatic assertion def: "A type of author statement supported by traceable reference that is used in an automatic assertion." [ECO:RCT] synonym: "traceable author statement used in automatic assertion" RELATED [] is_a: ECO:0000033 {is_inferred="true"} ! author statement supported by traceable reference is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion intersection_of: ECO:0000033 ! author statement supported by traceable reference intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007322 name: curator inference used in automatic assertion def: "A type of curator inference that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000205 ! curator inference is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion intersection_of: ECO:0000205 ! curator inference intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007323 name: inference from background scientific knowledge used in automatic assertion def: "A type of inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000001 {is_inferred="true"} ! inference from background scientific knowledge is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion intersection_of: ECO:0000001 ! inference from background scientific knowledge intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007324 name: no evidence data found used in automatic assertion def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000035 {is_inferred="true"} ! no evidence data found is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion intersection_of: ECO:0000035 ! no evidence data found intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007325 name: mutant phenotype evidence used in automatic assertion def: "A type of mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000015 {is_inferred="true"} ! mutant phenotype evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000015 ! mutant phenotype evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007326 name: genetic interaction evidence used in automatic assertion def: "A type of genetic interaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000011 {is_inferred="true"} ! genetic interaction evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000011 ! genetic interaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007327 name: genomic context evidence used in automatic assertion def: "A type of genomic context evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000177 {is_inferred="true"} ! genomic context evidence is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion intersection_of: ECO:0000177 ! genomic context evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007328 name: biological aspect of ancestor evidence used in automatic assertion def: "A type of biological aspect of ancestor evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000308 {is_inferred="true"} ! biological aspect of ancestor evidence is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion intersection_of: ECO:0000308 ! biological aspect of ancestor evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007329 name: biological aspect of descendant evidence used in automatic assertion def: "A type of biological aspect of descendant evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000214 {is_inferred="true"} ! biological aspect of descendant evidence is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion intersection_of: ECO:0000214 ! biological aspect of descendant evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007330 name: phylogenetic determination of loss of key residues evidence used in automatic assertion def: "A type of phylogenetic determination of loss of key residues evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000216 {is_inferred="true"} ! phylogenetic determination of loss of key residues evidence is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion intersection_of: ECO:0000216 ! phylogenetic determination of loss of key residues evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007331 name: rapid divergence from ancestral sequence evidence used in automatic assertion def: "A type of rapid divergence from ancestral sequence evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000215 {is_inferred="true"} ! rapid divergence from ancestral sequence evidence is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion intersection_of: ECO:0000215 ! rapid divergence from ancestral sequence evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007332 name: physical interaction evidence used in automatic assertion def: "A type of physical interaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000021 {is_inferred="true"} ! physical interaction evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000021 ! physical interaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007333 name: gene neighbors evidence used in automatic assertion def: "A type of gene neighbors evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000084 {is_inferred="true"} ! gene neighbors evidence is_a: ECO:0007327 {is_inferred="true"} ! genomic context evidence used in automatic assertion intersection_of: ECO:0000084 ! gene neighbors evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007334 name: Edman degradation evidence used in automatic assertion def: "A type of Edman degradation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001044 {is_inferred="true"} ! Edman degradation evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001044 ! Edman degradation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007335 name: 3D cell culture evidence used in automatic assertion def: "A type of 3D cell culture evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001001 {is_inferred="true"} ! 3D cell culture evidence is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0001001 ! 3D cell culture evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007336 name: 51Cr release assay evidence used in automatic assertion def: "A type of 51Cr release assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001004 {is_inferred="true"} ! 51Cr release assay evidence is_a: ECO:0007781 {is_inferred="true"} ! cytotoxicity assay evidence used in automatic assertion intersection_of: ECO:0001004 ! 51Cr release assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007337 name: 7-aminoactinomycin staining evidence used in automatic assertion def: "A type of 7-aminoactinomycin staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001005 {is_inferred="true"} ! 7-aminoactinomycin staining evidence is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion intersection_of: ECO:0001005 ! 7-aminoactinomycin staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007338 name: [3H]-thymidine incorporation assay evidence used in automatic assertion def: "A type of [3H]-thymidine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001003 {is_inferred="true"} ! [3H]-thymidine incorporation assay evidence is_a: ECO:0007776 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0001003 ! [3H]-thymidine incorporation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007339 name: [3H]arachidonic acid release assay evidence used in automatic assertion def: "A type of [3H]arachidonic acid release assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001002 {is_inferred="true"} ! [3H]arachidonic acid release assay evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001002 ! [3H]arachidonic acid release assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007340 name: adhesion assay evidence used in automatic assertion def: "A type of adhesion assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001006 {is_inferred="true"} ! adhesion assay evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001006 ! adhesion assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007341 name: adoptive cell transfer evidence used in automatic assertion def: "A type of adoptive cell transfer evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001007 {is_inferred="true"} ! adoptive cell transfer evidence is_a: ECO:0007793 {is_inferred="true"} ! ex vivo assay evidence used in automatic assertion intersection_of: ECO:0001007 ! adoptive cell transfer evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007342 name: alamarBlue assay evidence used in automatic assertion def: "A type of alamarBlue assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001008 {is_inferred="true"} ! alamarBlue assay evidence is_a: ECO:0007774 {is_inferred="true"} ! cell viability assay evidence used in automatic assertion intersection_of: ECO:0001008 ! alamarBlue assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007343 name: allograft transplantation phenotypic evidence used in automatic assertion def: "A type of allograft transplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "allograft transplantation evidence used in automatic assertion" EXACT [] is_a: ECO:0001009 {is_inferred="true"} ! allograft transplantation phenotypic evidence is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion intersection_of: ECO:0001009 ! allograft transplantation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007344 name: anion-exchange chromatography evidence used in automatic assertion def: "A type of anion-exchange chromatography evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001010 {is_inferred="true"} ! anion-exchange chromatography evidence is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion intersection_of: ECO:0001010 ! anion-exchange chromatography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007345 name: annexin-V staining evidence used in automatic assertion def: "A type of annexin-V staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001011 {is_inferred="true"} ! annexin-V staining evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001011 ! annexin-V staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007346 name: cognitive assay phenotypic evidence used in automatic assertion def: "A type of cognitive assay phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "behavioral assay evidence used in automatic assertion" EXACT [] is_a: ECO:0001012 {is_inferred="true"} ! cognitive assay phenotypic evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0001012 ! cognitive assay phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007347 name: blocking monoclonal antibody evidence used in automatic assertion def: "A type of blocking monoclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001013 {is_inferred="true"} ! blocking monoclonal antibody evidence is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion intersection_of: ECO:0001013 ! blocking monoclonal antibody evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007348 name: immunological assay evidence used in automatic assertion def: "A type of immunological assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000040 {is_inferred="true"} ! immunological assay evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0000040 ! immunological assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007349 name: blocking peptide evidence used in automatic assertion def: "A type of blocking peptide evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001014 {is_inferred="true"} ! blocking peptide evidence is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion intersection_of: ECO:0001014 ! blocking peptide evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007350 name: blocking polyclonal antibody evidence used in automatic assertion def: "A type of blocking polyclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001015 {is_inferred="true"} ! blocking polyclonal antibody evidence is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion intersection_of: ECO:0001015 ! blocking polyclonal antibody evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007351 name: blood test evidence used in automatic assertion def: "A type of blood test evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001016 {is_inferred="true"} ! blood test evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001016 ! blood test evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007352 name: Boyden chamber assay evidence used in automatic assertion def: "A type of Boyden chamber assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001017 {is_inferred="true"} ! Boyden chamber assay evidence is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion intersection_of: ECO:0001017 ! Boyden chamber assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007353 name: bromodeoxyuridine incorporation assay evidence used in automatic assertion def: "A type of bromodeoxyuridine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001018 {is_inferred="true"} ! bromodeoxyuridine incorporation assay evidence is_a: ECO:0007354 {is_inferred="true"} ! nucleotide analog incorporation assay evidence used in automatic assertion intersection_of: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007354 name: nucleotide analog incorporation assay evidence used in automatic assertion def: "A type of nucleotide analog incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001107 {is_inferred="true"} ! nucleotide analog incorporation assay evidence is_a: ECO:0007776 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0001107 ! nucleotide analog incorporation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007355 name: caspase assay evidence used in automatic assertion def: "A type of caspase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001019 {is_inferred="true"} ! caspase assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001019 ! caspase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007356 name: cell counting evidence used in automatic assertion def: "A type of cell counting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001020 {is_inferred="true"} ! cell counting evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001020 ! cell counting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007357 name: cell permeability assay evidence used in automatic assertion def: "A type of cell permeability assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001021 {is_inferred="true"} ! cell permeability assay evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001021 ! cell permeability assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007358 name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in automatic assertion def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001022 {is_inferred="true"} ! carboxyfluorescein diacetate succinimidyl ester staining evidence is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion intersection_of: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007359 name: chemiluminescence-linked immunoassay evidence used in automatic assertion def: "A type of chemiluminescence-linked immunoassay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001023 {is_inferred="true"} ! chemiluminescence-linked immunoassay evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001023 ! chemiluminescence-linked immunoassay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007360 name: chimeric protein phenotypic evidence used in automatic assertion def: "A type of chimeric protein phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "chimeric protein evidence used in automatic assertion" EXACT [] is_a: ECO:0001024 {is_inferred="true"} ! chimeric protein phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0001024 ! chimeric protein phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007361 name: co-electrophoresis evidence used in automatic assertion def: "A type of co-electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001025 {is_inferred="true"} ! co-electrophoresis evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0001025 ! co-electrophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007362 name: co-localization evidence used in automatic assertion def: "A type of co-localization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001026 {is_inferred="true"} ! co-localization evidence is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion intersection_of: ECO:0001026 ! co-localization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007363 name: imaging assay evidence used in automatic assertion def: "A type of imaging assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000324 ! imaging assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000324 ! imaging assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007364 name: co-sedimentation assay evidence used in automatic assertion def: "A type of co-sedimentation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001028 {is_inferred="true"} ! co-sedimentation assay evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0001028 ! co-sedimentation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007365 name: colony counting evidence used in automatic assertion def: "A type of colony counting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001027 {is_inferred="true"} ! colony counting evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001027 ! colony counting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007366 name: comet assay evidence used in automatic assertion def: "A type of comet assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001029 {is_inferred="true"} ! comet assay evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0001029 ! comet assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007367 name: conditional knockin evidence used in automatic assertion def: "A type of conditional knockin evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001031 {is_inferred="true"} ! conditional knockin evidence is_a: ECO:0007368 {is_inferred="true"} ! knockin evidence used in automatic assertion intersection_of: ECO:0001031 ! conditional knockin evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007368 name: knockin evidence used in automatic assertion def: "A type of knockin evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001090 {is_inferred="true"} ! knockin evidence is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion intersection_of: ECO:0001090 ! knockin evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007369 name: conditional knockout evidence used in automatic assertion def: "A type of conditional knockout evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001030 {is_inferred="true"} ! conditional knockout evidence is_a: ECO:0007370 {is_inferred="true"} ! knockout evidence used in automatic assertion intersection_of: ECO:0001030 ! conditional knockout evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007370 name: knockout evidence used in automatic assertion def: "A type of knockout evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001091 {is_inferred="true"} ! knockout phenotypic evidence is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion intersection_of: ECO:0001091 ! knockout phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007371 name: constitutively active mutant evidence used in automatic assertion def: "A type of constitutively active mutant evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001032 {is_inferred="true"} ! constitutively active mutant evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0001032 ! constitutively active mutant evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007372 name: cross-linking evidence used in automatic assertion def: "A type of cross-linking evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001033 {is_inferred="true"} ! cross-linking evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion intersection_of: ECO:0001033 ! cross-linking evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007373 name: protein binding evidence used in automatic assertion def: "A type of protein binding evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000024 {is_inferred="true"} ! protein binding evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0000024 ! protein binding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007374 name: crystallography evidence used in automatic assertion def: "A type of crystallography evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001034 {is_inferred="true"} ! crystallography evidence is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0001034 ! crystallography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007375 name: cytochemistry evidence used in automatic assertion def: "A type of cytochemistry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001035 {is_inferred="true"} ! cytochemistry evidence is_a: ECO:0007376 {is_inferred="true"} ! histochemistry evidence used in automatic assertion intersection_of: ECO:0001035 ! cytochemistry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007376 name: histochemistry evidence used in automatic assertion def: "A type of histochemistry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001050 {is_inferred="true"} ! histochemistry evidence is_a: ECO:0007389 {is_inferred="true"} ! histology evidence used in automatic assertion intersection_of: ECO:0001050 ! histochemistry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007377 name: cytochrome C release assay evidence used in automatic assertion def: "A type of cytochrome C release assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001036 {is_inferred="true"} ! cytochrome C release assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001036 ! cytochrome C release assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007378 name: 4',6-diamidino-2-phenylindole staining evidence used in automatic assertion def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001037 {is_inferred="true"} ! 4',6-diamidino-2-phenylindole staining evidence is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion intersection_of: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007379 name: deletion mutation phenotypic evidence used in automatic assertion def: "A type of deletion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "deletion mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0001038 {is_inferred="true"} ! deletion mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0001038 ! deletion mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007380 name: DNA laddering assay evidence used in automatic assertion def: "A type of DNA laddering assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001039 {is_inferred="true"} ! DNA laddering assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001039 ! DNA laddering assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007381 name: RNA dot blot assay evidence used in automatic assertion def: "A type of RNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001040 {is_inferred="true"} ! RNA dot blot assay evidence is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion intersection_of: ECO:0001040 ! RNA dot blot assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007382 name: dominant-negative mutant phenotypic evidence used in automatic assertion def: "A type of dominant-negative mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "dominant-negative mutant evidence used in automatic assertion" EXACT [] is_a: ECO:0001042 {is_inferred="true"} ! dominant-negative mutant phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0001042 ! dominant-negative mutant phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007383 name: eTag assay evidence used in automatic assertion def: "A type of eTag assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001045 {is_inferred="true"} ! eTag assay evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0001045 ! eTag assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007384 name: affinity evidence used in automatic assertion def: "A type of affinity evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000023 {is_inferred="true"} ! affinity evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0000023 ! affinity evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007385 name: filter binding assay evidence used in automatic assertion def: "A type of filter binding assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001046 {is_inferred="true"} ! filter binding assay evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0001046 ! filter binding assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007386 name: fluorescence in situ hybridization evidence used in automatic assertion def: "A type of fluorescence in situ hybridization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001047 {is_inferred="true"} ! fluorescence in situ hybridization evidence is_a: ECO:0001837 {is_inferred="true"} ! in situ hybridization evidence used in automatic assertion is_a: ECO:0007809 {is_inferred="true"} ! nucleic acid localization evidence used in automatic assertion intersection_of: ECO:0001047 ! fluorescence in situ hybridization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007387 name: fluorescence resonance energy transfer evidence used in automatic assertion def: "A type of fluorescence resonance energy transfer evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001048 {is_inferred="true"} ! fluorescence resonance energy transfer evidence is_a: ECO:0006328 {is_inferred="true"} ! dynamic fluorescence quenching evidence used in automatic assertion intersection_of: ECO:0001048 ! fluorescence resonance energy transfer evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007388 name: gel-filtration evidence used in automatic assertion def: "A type of gel-filtration evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001049 {is_inferred="true"} ! gel-filtration evidence is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion intersection_of: ECO:0001049 ! gel-filtration evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007389 name: histology evidence used in automatic assertion def: "A type of histology evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001051 {is_inferred="true"} ! histology evidence is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion intersection_of: ECO:0001051 ! histology evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007390 name: immunocytochemistry evidence used in automatic assertion def: "A type of immunocytochemistry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001053 {is_inferred="true"} ! immunocytochemistry evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001053 ! immunocytochemistry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007391 name: immunodepletion evidence used in automatic assertion def: "A type of immunodepletion evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001054 {is_inferred="true"} ! immunodepletion evidence is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion intersection_of: ECO:0001054 ! immunodepletion evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007392 name: immunohistochemistry evidence used in automatic assertion def: "A type of immunohistochemistry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001055 {is_inferred="true"} ! immunohistochemistry evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001055 ! immunohistochemistry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007393 name: in vitro acetylation assay evidence used in automatic assertion def: "A type of in vitro acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001057 {is_inferred="true"} ! in vitro acetylation assay evidence is_a: ECO:0007794 {is_inferred="true"} ! acetylation assay evidence used in automatic assertion intersection_of: ECO:0001057 ! in vitro acetylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007394 name: in vitro cleavage assay evidence used in automatic assertion def: "A type of in vitro cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001058 {is_inferred="true"} ! in vitro cleavage assay evidence is_a: ECO:0007782 {is_inferred="true"} ! cleavage assay evidence used in automatic assertion intersection_of: ECO:0001058 ! in vitro cleavage assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007395 name: in vitro deacetylation assay evidence used in automatic assertion def: "A type of in vitro deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001060 {is_inferred="true"} ! in vitro deacetylation assay evidence is_a: ECO:0007771 {is_inferred="true"} ! deacetylation assay evidence used in automatic assertion intersection_of: ECO:0001060 ! in vitro deacetylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007396 name: in vitro defarnesylation assay evidence used in automatic assertion def: "A type of in vitro defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001061 {is_inferred="true"} ! in vitro defarnesylation assay evidence is_a: ECO:0007790 {is_inferred="true"} ! defarnesylation assay evidence used in automatic assertion intersection_of: ECO:0001061 ! in vitro defarnesylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007397 name: in vitro demethylation assay evidence used in automatic assertion def: "A type of in vitro demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001062 {is_inferred="true"} ! in vitro demethylation assay evidence is_a: ECO:0007795 {is_inferred="true"} ! demethylation assay evidence used in automatic assertion intersection_of: ECO:0001062 ! in vitro demethylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007398 name: in vitro desumoylation assay evidence used in automatic assertion def: "A type of in vitro desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001063 {is_inferred="true"} ! in vitro desumoylation assay evidence is_a: ECO:0007802 {is_inferred="true"} ! desumoylation assay evidence used in automatic assertion intersection_of: ECO:0001063 ! in vitro desumoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007399 name: in vitro deubiquitination assay evidence used in automatic assertion def: "A type of in vitro deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001059 {is_inferred="true"} ! in vitro deubiquitination assay evidence is_a: ECO:0007791 {is_inferred="true"} ! deubiquitination assay evidence used in automatic assertion intersection_of: ECO:0001059 ! in vitro deubiquitination assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007400 name: in vitro farnesylation assay evidence used in automatic assertion def: "A type of in vitro farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001064 {is_inferred="true"} ! in vitro farnesylation assay evidence is_a: ECO:0007800 {is_inferred="true"} ! farnesylation assay evidence used in automatic assertion intersection_of: ECO:0001064 ! in vitro farnesylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007401 name: in vitro methylation assay evidence used in automatic assertion def: "A type of in vitro methylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001065 {is_inferred="true"} ! in vitro methylation assay evidence is_a: ECO:0007783 {is_inferred="true"} ! methylation assay evidence used in automatic assertion intersection_of: ECO:0001065 ! in vitro methylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007402 name: in vitro palmitoylation assay evidence used in automatic assertion def: "A type of in vitro palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001066 {is_inferred="true"} ! in vitro palmitoylation assay evidence is_a: ECO:0007792 {is_inferred="true"} ! palmitoylation assay evidence used in automatic assertion intersection_of: ECO:0001066 ! in vitro palmitoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007403 name: in vitro phosphatase assay evidence used in automatic assertion def: "A type of in vitro phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001067 {is_inferred="true"} ! in vitro phosphatase assay evidence is_a: ECO:0007772 {is_inferred="true"} ! phosphatase assay evidence used in automatic assertion intersection_of: ECO:0001067 ! in vitro phosphatase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007404 name: in vitro polyADP-ribosylation assay evidence used in automatic assertion def: "A type of in vitro polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001069 {is_inferred="true"} ! in vitro polyADP-ribosylation assay evidence is_a: ECO:0007797 {is_inferred="true"} ! polyADP-ribosylation assay evidence used in automatic assertion intersection_of: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007405 name: in vitro protein kinase assay evidence used in automatic assertion def: "A type of in vitro protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001068 {is_inferred="true"} ! in vitro protein kinase assay evidence is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion intersection_of: ECO:0001068 ! in vitro protein kinase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007406 name: in vitro sumoylation assay evidence used in automatic assertion def: "A type of in vitro sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001070 {is_inferred="true"} ! in vitro sumoylation assay evidence is_a: ECO:0007788 {is_inferred="true"} ! sumoylation assay evidence used in automatic assertion intersection_of: ECO:0001070 ! in vitro sumoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007407 name: in vitro transcription assay evidence used in automatic assertion def: "A type of in vitro transcription assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001071 {is_inferred="true"} ! in vitro transcription assay evidence is_a: ECO:0007768 {is_inferred="true"} ! transcription assay evidence used in automatic assertion intersection_of: ECO:0001071 ! in vitro transcription assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007408 name: in vitro translation assay evidence used in automatic assertion def: "A type of in vitro translation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001072 {is_inferred="true"} ! in vitro translation assay evidence is_a: ECO:0007799 {is_inferred="true"} ! translation assay evidence used in automatic assertion intersection_of: ECO:0001072 ! in vitro translation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007409 name: in vitro ubiquitination assay evidence used in automatic assertion def: "A type of in vitro ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001073 {is_inferred="true"} ! in vitro ubiquitination assay evidence is_a: ECO:0007801 {is_inferred="true"} ! ubiquitination assay evidence used in automatic assertion intersection_of: ECO:0001073 ! in vitro ubiquitination assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007410 name: in vivo acetylation assay evidence used in automatic assertion def: "A type of in vivo acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001074 {is_inferred="true"} ! in vivo acetylation assay evidence is_a: ECO:0007794 {is_inferred="true"} ! acetylation assay evidence used in automatic assertion intersection_of: ECO:0001074 ! in vivo acetylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007411 name: in vivo cleavage assay evidence used in automatic assertion def: "A type of in vivo cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001075 {is_inferred="true"} ! in vivo cleavage assay evidence is_a: ECO:0007782 {is_inferred="true"} ! cleavage assay evidence used in automatic assertion intersection_of: ECO:0001075 ! in vivo cleavage assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007412 name: in vivo deacetylation assay evidence used in automatic assertion def: "A type of in vivo deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001076 {is_inferred="true"} ! in vivo deacetylation assay evidence is_a: ECO:0007771 {is_inferred="true"} ! deacetylation assay evidence used in automatic assertion intersection_of: ECO:0001076 ! in vivo deacetylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007413 name: in vivo defarnesylation assay evidence used in automatic assertion def: "A type of in vivo defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001077 {is_inferred="true"} ! in vivo defarnesylation assay evidence is_a: ECO:0007790 {is_inferred="true"} ! defarnesylation assay evidence used in automatic assertion intersection_of: ECO:0001077 ! in vivo defarnesylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007414 name: in vivo demethylation assay evidence used in automatic assertion def: "A type of in vivo demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001078 {is_inferred="true"} ! in vivo demethylation assay evidence is_a: ECO:0007795 {is_inferred="true"} ! demethylation assay evidence used in automatic assertion intersection_of: ECO:0001078 ! in vivo demethylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007415 name: in vivo desumoylation assay evidence used in automatic assertion def: "A type of in vivo desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001080 {is_inferred="true"} ! in vivo desumoylation assay evidence is_a: ECO:0007802 {is_inferred="true"} ! desumoylation assay evidence used in automatic assertion intersection_of: ECO:0001080 ! in vivo desumoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007416 name: in vivo deubiquitination assay evidence used in automatic assertion def: "A type of in vivo deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001079 {is_inferred="true"} ! in vivo deubiquitination assay evidence is_a: ECO:0007791 {is_inferred="true"} ! deubiquitination assay evidence used in automatic assertion intersection_of: ECO:0001079 ! in vivo deubiquitination assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007417 name: in vivo farnesylation assay evidence used in automatic assertion def: "A type of in vivo farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001081 {is_inferred="true"} ! in vivo farnesylation assay evidence is_a: ECO:0007800 {is_inferred="true"} ! farnesylation assay evidence used in automatic assertion intersection_of: ECO:0001081 ! in vivo farnesylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007418 name: in vivo methylation assay evidence used in automatic assertion def: "A type of in vivo methylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001082 {is_inferred="true"} ! in vivo methylation assay evidence is_a: ECO:0007783 {is_inferred="true"} ! methylation assay evidence used in automatic assertion intersection_of: ECO:0001082 ! in vivo methylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007419 name: in vivo palmitoylation assay evidence used in automatic assertion def: "A type of in vivo palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001083 {is_inferred="true"} ! in vivo palmitoylation assay evidence is_a: ECO:0007792 {is_inferred="true"} ! palmitoylation assay evidence used in automatic assertion intersection_of: ECO:0001083 ! in vivo palmitoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007420 name: in vivo phosphatase assay evidence used in automatic assertion def: "A type of in vivo phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001084 {is_inferred="true"} ! in vivo phosphatase assay evidence is_a: ECO:0007772 {is_inferred="true"} ! phosphatase assay evidence used in automatic assertion intersection_of: ECO:0001084 ! in vivo phosphatase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007421 name: in vivo protein kinase assay evidence used in automatic assertion def: "A type of in vivo protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001085 {is_inferred="true"} ! in vivo protein kinase assay evidence is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion intersection_of: ECO:0001085 ! in vivo protein kinase assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007422 name: in vivo sumoylation assay evidence used in automatic assertion def: "A type of in vivo sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001086 {is_inferred="true"} ! in vivo sumoylation assay evidence is_a: ECO:0007788 {is_inferred="true"} ! sumoylation assay evidence used in automatic assertion intersection_of: ECO:0001086 ! in vivo sumoylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007423 name: in vivo transcription assay evidence used in automatic assertion def: "A type of in vivo transcription assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001087 {is_inferred="true"} ! in vivo transcription assay evidence is_a: ECO:0007768 {is_inferred="true"} ! transcription assay evidence used in automatic assertion intersection_of: ECO:0001087 ! in vivo transcription assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007424 name: in vivo translation assay evidence used in automatic assertion def: "A type of in vivo translation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001088 {is_inferred="true"} ! in vivo translation assay evidence is_a: ECO:0007799 {is_inferred="true"} ! translation assay evidence used in automatic assertion intersection_of: ECO:0001088 ! in vivo translation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007425 name: in vivo ubiquitination assay evidence used in automatic assertion def: "A type of in vivo ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001089 {is_inferred="true"} ! in vivo ubiquitination assay evidence is_a: ECO:0007801 {is_inferred="true"} ! ubiquitination assay evidence used in automatic assertion intersection_of: ECO:0001089 ! in vivo ubiquitination assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007426 name: induced mutation evidence used in automatic assertion def: "A type of induced mutation evidence that is used in an automatic assertion." [ECO:RCT] is_obsolete: true created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007427 name: genetic transformation evidence used in automatic assertion def: "A type of genetic transformation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005027 {is_inferred="true"} ! genetic transformation evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005027 ! genetic transformation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007428 name: lipid binding assay evidence used in automatic assertion def: "A type of lipid binding assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001092 {is_inferred="true"} ! lipid binding assay evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0001092 ! lipid binding assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007429 name: luminescence-based mammalian interactome mapping assay evidence used in automatic assertion def: "A type of luminescence-based mammalian interactome mapping assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001093 {is_inferred="true"} ! luminescence-based mammalian interactome mapping assay evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007430 name: macroscopy evidence used in automatic assertion def: "A type of macroscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001094 {is_inferred="true"} ! macroscopy evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0001094 ! macroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007431 name: mammalian 2-hybrid assay evidence used in automatic assertion def: "A type of mammalian 2-hybrid assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001095 {is_inferred="true"} ! mammalian 2-hybrid assay evidence is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion intersection_of: ECO:0001095 ! mammalian 2-hybrid assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007432 name: bait-prey hybrid interaction evidence used in automatic assertion def: "A type of bait-prey hybrid interaction evidence that is used in an automatic assertion." [ECO:RCT] synonym: "bait-prey protein pull-down evidence" EXACT [] is_a: ECO:0000025 {is_inferred="true"} ! bait-prey hybrid interaction evidence is_a: ECO:0006393 {is_inferred="true"} ! bait-prey interaction evidence used in automatic assertion intersection_of: ECO:0000025 ! bait-prey hybrid interaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007433 name: mass spectrometry evidence used in automatic assertion def: "A type of mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001096 {is_inferred="true"} ! mass spectrometry evidence is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion intersection_of: ECO:0001096 ! mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007434 name: medical imaging evidence used in automatic assertion def: "A type of medical imaging evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001097 {is_inferred="true"} ! medical imaging evidence is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion intersection_of: ECO:0001097 ! medical imaging evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007435 name: microscopy evidence used in automatic assertion def: "A type of microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001098 {is_inferred="true"} ! microscopy evidence is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion intersection_of: ECO:0001098 ! microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007436 name: motility wound healing assay evidence used in automatic assertion def: "A type of motility wound healing assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001099 {is_inferred="true"} ! motility wound healing assay evidence is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion intersection_of: ECO:0001099 ! motility wound healing assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007437 name: MTS assay evidence used in automatic assertion def: "A type of MTS assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001100 {is_inferred="true"} ! MTS assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001100 ! MTS assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007438 name: MTT assay evidence used in automatic assertion def: "A type of MTT assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001101 {is_inferred="true"} ! MTT assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001101 ! MTT assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007439 name: multiplex bead-based immunoassay evidence used in automatic assertion def: "A type of multiplex bead-based immunoassay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001102 {is_inferred="true"} ! multiplex bead-based immunoassay evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001102 ! multiplex bead-based immunoassay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007440 name: natural variation mutant evidence used in automatic assertion def: "A type of natural variation mutant evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001103 {is_inferred="true"} ! natural variation mutant evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0001103 ! natural variation mutant evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007441 name: nuclear magnetic resonance evidence used in automatic assertion def: "A type of nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001105 {is_inferred="true"} ! nuclear magnetic resonance evidence is_a: ECO:0006286 {is_inferred="true"} ! magnetic resonance evidence used in automatic assertion intersection_of: ECO:0001105 ! nuclear magnetic resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007442 name: nuclear fragmentation evidence used in automatic assertion def: "A type of nuclear fragmentation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001104 {is_inferred="true"} ! nuclear fragmentation evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001104 ! nuclear fragmentation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007443 name: phage display evidence used in automatic assertion def: "A type of phage display evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001108 {is_inferred="true"} ! phage display evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion intersection_of: ECO:0001108 ! phage display evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007444 name: phosphoamino acid analysis evidence used in automatic assertion def: "A type of phosphoamino acid analysis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001109 {is_inferred="true"} ! phosphoamino acid analysis evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001109 ! phosphoamino acid analysis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007445 name: peptide affinity enrichment evidence used in automatic assertion def: "A type of peptide affinity enrichment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001110 {is_inferred="true"} ! peptide affinity enrichment evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0001110 ! peptide affinity enrichment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007446 name: peptide array evidence used in automatic assertion def: "A type of peptide array evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001112 {is_inferred="true"} ! peptide array evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion intersection_of: ECO:0001112 ! peptide array evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007447 name: physical examination evidence used in automatic assertion def: "A type of physical examination evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001111 {is_inferred="true"} ! physical examination evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001111 ! physical examination evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007448 name: point mutation phenotypic evidence used in automatic assertion def: "A type of point mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "point mutation evidence used in automatic assertion" EXACT [] is_a: ECO:0001113 {is_inferred="true"} ! point mutation phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0001113 ! point mutation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007449 name: propidium iodide staining evidence used in automatic assertion def: "A type of propidium iodide staining evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001114 {is_inferred="true"} ! propidium iodide staining evidence is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion intersection_of: ECO:0001114 ! propidium iodide staining evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007450 name: fluorescence evidence used in automatic assertion def: "A type of fluorescence evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001115 {is_inferred="true"} ! fluorescence evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001115 ! fluorescence evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007451 name: protein dot blot assay evidence used in automatic assertion def: "A type of protein dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001116 {is_inferred="true"} ! protein dot blot assay evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001116 ! protein dot blot assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007452 name: protein microarray evidence used in automatic assertion def: "A type of protein microarray evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001117 {is_inferred="true"} ! protein microarray evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001117 ! protein microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007453 name: protein sequencing assay evidence used in automatic assertion def: "A type of protein sequencing assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001118 {is_inferred="true"} ! protein sequencing assay evidence is_a: ECO:0007812 {is_inferred="true"} ! sequencing assay evidence used in automatic assertion intersection_of: ECO:0001118 ! protein sequencing assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007454 name: quantitative mass spectrometry evidence used in automatic assertion def: "A type of quantitative mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001119 {is_inferred="true"} ! quantitative mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0001119 ! quantitative mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007455 name: radioisotope assay evidence used in automatic assertion def: "A type of radioisotope assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001120 {is_inferred="true"} ! radioisotope assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001120 ! radioisotope assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007456 name: radioimmunoassay evidence used in automatic assertion def: "A type of radioimmunoassay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001121 {is_inferred="true"} ! radioimmunoassay evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0001121 ! radioimmunoassay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007457 name: restriction fragment detection evidence used in automatic assertion def: "A type of restriction fragment detection evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001124 {is_inferred="true"} ! restriction fragment detection evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0001124 ! restriction fragment detection evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007458 name: spectrophotometry evidence used in automatic assertion def: "A type of spectrophotometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001126 {is_inferred="true"} ! spectrophotometry evidence is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion intersection_of: ECO:0001126 ! spectrophotometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007459 name: syngeneic transplantation experiment evidence used in automatic assertion def: "A type of syngeneic transplantation experiment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001128 {is_inferred="true"} ! syngeneic transplantation experiment evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0001128 ! syngeneic transplantation experiment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007460 name: xenotransplantation phenotypic evidence used in automatic assertion def: "A type of xenotransplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "xenotransplantation experiment evidence used in automatic assertion" EXACT [] is_a: ECO:0001137 {is_inferred="true"} ! xenotransplantation phenotypic evidence is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion intersection_of: ECO:0001137 ! xenotransplantation phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007461 name: WST-1 assay evidence used in automatic assertion def: "A type of WST-1 assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001136 {is_inferred="true"} ! WST-1 assay evidence is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion intersection_of: ECO:0001136 ! WST-1 assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007462 name: urine test evidence used in automatic assertion def: "A type of urine test evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001134 {is_inferred="true"} ! urine test evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001134 ! urine test evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007463 name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in automatic assertion def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001133 {is_inferred="true"} ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion intersection_of: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007464 name: tryptic phosphopeptide mapping assay evidence used in automatic assertion def: "A type of tryptic phosphopeptide mapping assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001132 {is_inferred="true"} ! tryptic phosphopeptide mapping assay evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007465 name: transgenic organism evidence used in automatic assertion def: "A type of transgenic organism evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001131 {is_inferred="true"} ! transgenic organism evidence is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion intersection_of: ECO:0001131 ! transgenic organism evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007466 name: tissue microarray evidence used in automatic assertion def: "A type of tissue microarray evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001130 {is_inferred="true"} ! tissue microarray evidence is_a: ECO:0007375 {is_inferred="true"} ! cytochemistry evidence used in automatic assertion intersection_of: ECO:0001130 ! tissue microarray evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007467 name: TACE activity assay evidence used in automatic assertion def: "A type of TACE activity assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001129 {is_inferred="true"} ! TACE activity assay evidence is_a: ECO:0007468 {is_inferred="true"} ! enzymatic activity assay evidence used in automatic assertion intersection_of: ECO:0001129 ! TACE activity assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007468 name: enzymatic activity assay evidence used in automatic assertion def: "A type of enzyme assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000005 {is_inferred="true"} ! enzymatic activity assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000005 ! enzymatic activity assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007469 name: surface plasmon resonance evidence used in automatic assertion def: "A type of surface plasmon resonance evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001127 {is_inferred="true"} ! surface plasmon resonance evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0001127 ! surface plasmon resonance evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007470 name: restriction landmark genomic scanning evidence used in automatic assertion def: "A type of restriction landmark genomic scanning evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000089 {is_inferred="true"} ! restriction landmark genomic scanning evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0000089 ! restriction landmark genomic scanning evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007471 name: resonant mirror biosensor evidence used in automatic assertion def: "A type of resonant mirror biosensor evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001123 {is_inferred="true"} ! resonant mirror biosensor evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0001123 ! resonant mirror biosensor evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007472 name: high-performance liquid chromatography evidence used in automatic assertion def: "A type of high-performance liquid chromatography evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001052 {is_inferred="true"} ! high-performance liquid chromatography evidence is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion intersection_of: ECO:0001052 ! high-performance liquid chromatography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007473 name: ectopic expression evidence used in automatic assertion def: "A type of ectopic expression evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000017 {is_inferred="true"} ! ectopic expression evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0000017 ! ectopic expression evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007474 name: electrophoretic mobility shift assay evidence used in automatic assertion def: "A type of electrophoretic mobility shift assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000096 {is_inferred="true"} ! electrophoretic mobility shift assay evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0000096 ! electrophoretic mobility shift assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007475 name: reverse transcription polymerase chain reaction evidence used in automatic assertion alt_id: ECO:000108 def: "A type of reverse transcription polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000109 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion intersection_of: ECO:0000109 ! reverse transcription polymerase chain reaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007476 name: in vivo polyADP-ribosylation assay evidence used in automatic assertion def: "A type of in vivo polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005500 {is_inferred="true"} ! in vivo polyADP-ribosylation assay evidence is_a: ECO:0007797 {is_inferred="true"} ! polyADP-ribosylation assay evidence used in automatic assertion intersection_of: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007477 name: DNA dot blot assay evidence used in automatic assertion def: "A type of DNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005522 {is_inferred="true"} ! DNA dot blot assay evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0005522 ! DNA dot blot assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007478 name: random mutagenesis evidence used in automatic assertion def: "A type of random mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005529 {is_inferred="true"} ! random mutagenesis phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005529 ! random mutagenesis phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007479 name: biological system reconstruction evidence by experimental evidence from single species used in automatic assertion def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005553 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence from single species is_a: ECO:0007828 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in automatic assertion intersection_of: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007480 name: biological system reconstruction evidence by experimental evidence from mixed species used in automatic assertion def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005552 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence from mixed species is_a: ECO:0007828 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in automatic assertion intersection_of: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007481 name: biological system reconstruction evidence based on orthology evidence used in automatic assertion def: "A type of biological system reconstruction evidence based on orthology evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005545 {is_inferred="true"} ! biological system reconstruction evidence based on orthology evidence is_a: ECO:0007482 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence used in automatic assertion intersection_of: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007482 name: biological system reconstruction evidence based on homology evidence used in automatic assertion def: "A type of biological system reconstruction evidence based on homology evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005549 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence is_a: ECO:0007827 {is_inferred="true"} ! biological system reconstruction evidence used in automatic assertion intersection_of: ECO:0005549 ! biological system reconstruction evidence based on homology evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007483 name: biological system reconstruction evidence based on paralogy evidence used in automatic assertion def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005550 {is_inferred="true"} ! biological system reconstruction evidence based on paralogy evidence is_a: ECO:0007482 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence used in automatic assertion intersection_of: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007484 name: biological system reconstruction evidence based on inference from background scientific knowledge used in automatic assertion def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005548 {is_inferred="true"} ! biological system reconstruction evidence based on inference from background scientific knowledge is_a: ECO:0007827 {is_inferred="true"} ! biological system reconstruction evidence used in automatic assertion intersection_of: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007485 name: immunogold labelling evidence used in automatic assertion def: "A type of immunogold labelling evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000090 {is_inferred="true"} ! immunogold labelling evidence is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion intersection_of: ECO:0000090 ! immunogold labelling evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007486 name: immunolocalization evidence used in automatic assertion def: "A type of immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000087 {is_inferred="true"} ! immunolocalization evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion intersection_of: ECO:0000087 ! immunolocalization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007487 name: flow cytometry evidence used in automatic assertion def: "A type of flow cytometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000268 {is_inferred="true"} ! flow cytometry evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000268 ! flow cytometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007488 name: enzyme-linked immunoabsorbent assay evidence used in automatic assertion def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000267 {is_inferred="true"} ! enzyme-linked immunoabsorbent assay evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007489 name: high throughput mass spectrometry evidence used in automatic assertion def: "A type of high throughput mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005585 {is_inferred="true"} ! high throughput mass spectrometry evidence is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion intersection_of: ECO:0005585 ! high throughput mass spectrometry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007490 name: confocal microscopy evidence used in automatic assertion def: "A type of confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005587 {is_inferred="true"} ! confocal microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion intersection_of: ECO:0005587 ! confocal microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007491 name: wide-field microscopy evidence used in automatic assertion def: "A type of wide-field microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005588 {is_inferred="true"} ! wide-field microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion intersection_of: ECO:0005588 ! wide-field microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007492 name: immunogold labelling electron microscopy assay evidence used in automatic assertion def: "A type of immunogold labelling electron microscopy assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005591 {is_inferred="true"} ! immunogold labelling electron microscopy assay evidence is_a: ECO:0007485 {is_inferred="true"} ! immunogold labelling evidence used in automatic assertion is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion intersection_of: ECO:0005591 ! immunogold labelling electron microscopy assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007493 name: electron microscopy evidence used in automatic assertion def: "A type of electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005033 {is_inferred="true"} ! electron microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion intersection_of: ECO:0005033 ! electron microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007494 name: immunoperoxidase immunolocalization evidence used in automatic assertion def: "A type of immunoperoxidase immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005594 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion intersection_of: ECO:0005594 ! immunoperoxidase immunolocalization evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007495 name: immunoperoxidase immunolocalization electron microscopy evidence used in automatic assertion def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005596 {is_inferred="true"} ! immunoperoxidase immunolocalization electron microscopy evidence is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion is_a: ECO:0007494 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence used in automatic assertion intersection_of: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007496 name: immunofluorescence confocal microscopy evidence used in automatic assertion def: "A type of immunofluorescence confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005600 {is_inferred="true"} ! immunofluorescence confocal microscopy evidence is_a: ECO:0007490 {is_inferred="true"} ! confocal microscopy evidence used in automatic assertion is_a: ECO:0007497 {is_inferred="true"} ! immunofluorescence evidence used in automatic assertion intersection_of: ECO:0005600 ! immunofluorescence confocal microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007497 name: immunofluorescence evidence used in automatic assertion def: "A type of immunofluorescence evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000007 {is_inferred="true"} ! immunofluorescence evidence is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion intersection_of: ECO:0000007 ! immunofluorescence evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007498 name: two-dimensional polyacrylamide gel electrophoresis evidence used in automatic assertion def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005503 {is_inferred="true"} ! two-dimensional polyacrylamide gel electrophoresis evidence is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion intersection_of: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007499 name: alkaline phosphatase reporter gene assay evidence used in automatic assertion def: "A type of alkaline phosphatase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001801 {is_inferred="true"} ! alkaline phosphatase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007500 name: beta-galactosidase reporter gene assay evidence used in automatic assertion def: "A type of beta-galactosidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] synonym: "LacZ transcript localization evidence used in automatic assertion" EXACT [] is_a: ECO:0001802 {is_inferred="true"} ! beta-galactosidase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001802 ! beta-galactosidase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007501 name: chloramphenicol acetyltransferase reporter gene assay evidence used in automatic assertion def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001803 {is_inferred="true"} ! chloramphenicol acetyltransferase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007502 name: chromatin immunoprecipitation-PCR evidence used in automatic assertion def: "A type of chromatin immunoprecipitation-PCR evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000227 {is_inferred="true"} ! chromatin immunoprecipitation-PCR evidence is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion intersection_of: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007503 name: immunoprecipitation evidence used in automatic assertion def: "A type of immunoprecipitation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000085 {is_inferred="true"} ! immunoprecipitation evidence is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion intersection_of: ECO:0000085 ! immunoprecipitation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007504 name: copper-phenanthroline footprinting evidence used in automatic assertion def: "A type of copper-phenanthroline footprinting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001815 {is_inferred="true"} ! copper-phenanthroline footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001815 ! copper-phenanthroline footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007505 name: nucleic acid binding evidence used in automatic assertion def: "A type of nucleic acid binding evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000136 {is_inferred="true"} ! nucleic acid binding evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0000136 ! nucleic acid binding evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007506 name: DNA affinity chromatography evidence used in automatic assertion def: "A type of DNA affinity chromatography evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001809 {is_inferred="true"} ! DNA affinity chromatography evidence is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion intersection_of: ECO:0001809 ! DNA affinity chromatography evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007507 name: DNAse footprinting evidence used in automatic assertion def: "A type of DNAse footprinting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001810 {is_inferred="true"} ! DNAse footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001810 ! DNAse footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007508 name: fluorescence anisotropy evidence used in automatic assertion def: "A type of fluorescence anisotropy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001811 {is_inferred="true"} ! fluorescence anisotropy evidence is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion intersection_of: ECO:0001811 ! fluorescence anisotropy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007509 name: ferric uptake regulator titration assay evidence used in automatic assertion def: "A type of ferric uptake regulator titration assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001829 {is_inferred="true"} ! ferric uptake regulator titration assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001829 ! ferric uptake regulator titration assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007510 name: systematic evolution of ligands by exponential amplification evidence used in automatic assertion def: "A type of systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000293 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion intersection_of: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007511 name: glutathione S-transferase pull-down assay evidence used in automatic assertion def: "A type of glutathione S-transferase pull-down assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001817 {is_inferred="true"} ! glutathione S-transferase pull-down assay evidence is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion intersection_of: ECO:0001817 ! glutathione S-transferase pull-down assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007512 name: beta-glucuronidase reporter gene assay evidence used in automatic assertion def: "A type of beta-glucuronidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001804 {is_inferred="true"} ! beta-glucuronidase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007513 name: heteronuclear single quantum coherence spectroscopy evidence used in automatic assertion def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001813 {is_inferred="true"} ! heteronuclear single quantum coherence spectroscopy evidence is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion intersection_of: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007514 name: hydroxyl-radical footprinting evidence used in automatic assertion def: "A type of hydroxyl-radical footprinting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001818 {is_inferred="true"} ! hydroxyl-radical footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001818 ! hydroxyl-radical footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007515 name: isothermal titration calorimetry evidence used in automatic assertion def: "A type of isothermal titration calorimetry evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001825 {is_inferred="true"} ! isothermal titration calorimetry evidence is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion intersection_of: ECO:0001825 ! isothermal titration calorimetry evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007516 name: luciferase reporter gene assay evidence used in automatic assertion def: "A type of luciferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001805 {is_inferred="true"} ! luciferase reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0001805 ! luciferase reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007517 name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in automatic assertion def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001814 {is_inferred="true"} ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007518 name: northern blot evidence used in automatic assertion def: "A type of northern blot evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000106 {is_inferred="true"} ! northern blot evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0000106 ! northern blot evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007519 name: methylation interference footprinting evidence used in automatic assertion def: "A type of methylation interference footprinting evidence that is used in an automatic assertion." [ECO:RCT] synonym: "premethylation interference footprinting evidence used in automatic assertion" EXACT [] is_a: ECO:0001827 {is_inferred="true"} ! methylation interference footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001827 ! methylation interference footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007520 name: primer extension assay evidence used in automatic assertion def: "A type of primer extension assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001819 {is_inferred="true"} ! primer extension assay evidence is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion intersection_of: ECO:0001819 ! primer extension assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007521 name: quantitative polymerase chain reaction evidence used in automatic assertion def: "A type of quantitative polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000231 {is_inferred="true"} ! quantitative polymerase chain reaction evidence is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion intersection_of: ECO:0000231 ! quantitative polymerase chain reaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007522 name: rapid amplification of cDNA ends polymerase chain reaction evidence used in automatic assertion def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001820 {is_inferred="true"} ! rapid amplification of cDNA ends polymerase chain reaction evidence is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion intersection_of: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007523 name: S1 nuclease protection assay evidence used in automatic assertion def: "A type of S1 nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005521 {is_inferred="true"} ! S1 nuclease protection assay evidence is_a: ECO:0007316 {is_inferred="true"} ! nuclease protection assay evidence used in automatic assertion intersection_of: ECO:0005521 ! S1 nuclease protection assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007524 name: site-directed mutagenesis phenotypic evidence used in automatic assertion def: "A type of site-directed mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] synonym: "site-directed mutagenesis evidence used in automatic assertion" EXACT [] is_a: ECO:0005528 {is_inferred="true"} ! site-directed mutagenesis phenotypic evidence is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion intersection_of: ECO:0005528 ! site-directed mutagenesis phenotypic evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007525 name: survival rate analysis evidence used in automatic assertion def: "A type of survival rate analysis evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001822 {is_inferred="true"} ! survival rate analysis evidence is_a: ECO:0007370 {is_inferred="true"} ! knockout evidence used in automatic assertion intersection_of: ECO:0001822 ! survival rate analysis evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007526 name: ultraviolet light footprinting evidence used in automatic assertion def: "A type of ultraviolet light footprinting evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0001826 {is_inferred="true"} ! ultraviolet light footprinting evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0001826 ! ultraviolet light footprinting evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007527 name: xylE reporter gene assay evidence used in automatic assertion def: "A type of xylE reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005537 {is_inferred="true"} ! xylE reporter gene assay evidence is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0005537 ! xylE reporter gene assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007528 name: ad-hoc qualitative phenotype observation evidence used in automatic assertion def: "A type of ad-hoc qualitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005673 {is_inferred="true"} ! ad-hoc qualitative phenotype observation evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007529 name: ad-hoc quantitative phenotype observation evidence used in automatic assertion def: "A type of ad-hoc quantitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005675 {is_inferred="true"} ! ad-hoc quantitative phenotype observation evidence is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion intersection_of: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007530 name: cell transfection experiment evidence used in automatic assertion def: "A type of cell transfection experiment evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005606 {is_inferred="true"} ! cell transfection experiment evidence is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion intersection_of: ECO:0005606 ! cell transfection experiment evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007531 name: yeast 2-hybrid evidence used in automatic assertion def: "A type of yeast 2-hybrid evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000068 {is_inferred="true"} ! yeast 2-hybrid evidence is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion intersection_of: ECO:0000068 ! yeast 2-hybrid evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007532 name: Cya fusion reporter assay evidence used in automatic assertion def: "A type of Cya fusion reporter assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0006001 {is_inferred="true"} ! Cya fusion reporter assay evidence is_a: ECO:0007473 {is_inferred="true"} ! ectopic expression evidence used in automatic assertion is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion intersection_of: ECO:0006001 ! Cya fusion reporter assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007533 name: super-resolution microscopy evidence used in automatic assertion def: "A type of super-resolution microscopy evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0005578 {is_inferred="true"} ! super-resolution microscopy evidence is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion intersection_of: ECO:0005578 ! super-resolution microscopy evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007534 name: fractionation evidence used in automatic assertion def: "A type of fractionation evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000100 {is_inferred="true"} ! fractionation evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000100 ! fractionation evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007535 name: electrophysiology assay evidence used in automatic assertion def: "A type of electrophysiology assay evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0000164 {is_inferred="true"} ! electrophysiology assay evidence is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion intersection_of: ECO:0000164 ! electrophysiology assay evidence intersection_of: used_in ECO:0000203 ! automatic assertion property_value: ECO:9000002 "IEA" xsd:string created_by: rctauber creation_date: 2018-05-14T09:47:55Z [Term] id: ECO:0007536 name: mRNA interactome capture evidence used in automatic assertion def: "A type of mRNA interactome capture evidence that is used in an automatic assertion." [ECO:RCT] is_a: ECO:0006010 {is_inferred="true"} ! mRNA interactome capture evidence is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion intersection_of: ECO:0006010 ! mRNA interactome capture evidence intersection_