--- title: 'Our Lab Wiki Is Now Public' date: 2026-10-19 description: 'Fourteen years of Finkelstein Lab protocols, recipes and tribal knowledge.' tags: - 'protocols' - 'open-science' - 'academia' --- I've open-sourced 14 years of lab protocols and accumulated knowledge at [finkelsteinlab.org/wiki](https://finkelsteinlab.org/wiki/). ## Where to start If you are coming from outside the lab, these are the pages most likely to be useful: - [Protein purification](https://finkelsteinlab.org/wiki/protocols/protein-purification/). Some useful starting points: CRISPR nucleases ([Cas9](https://finkelsteinlab.org/wiki/protocols/protein-purification/cas9-purification/), [Cas12a](https://finkelsteinlab.org/wiki/protocols/protein-purification/cas12a-cpf1-purification/), [Cas13d](https://finkelsteinlab.org/wiki/protocols/protein-purification/cas13d-snap-escas13d-purification/), [Cascade](https://finkelsteinlab.org/wiki/protocols/protein-purification/t-fusca-cascade-purification/), [Cas3](https://finkelsteinlab.org/wiki/protocols/protein-purification/t-fusca-cas3-purification/)), DNA repair and replication proteins ([human](https://finkelsteinlab.org/wiki/protocols/protein-purification/human-rpa-purification/) and [yeast](https://finkelsteinlab.org/wiki/protocols/protein-purification/scrpa-gfp-his6-purification/) RPA, [Exo1](https://finkelsteinlab.org/wiki/protocols/protein-purification/exo1-purification/), [Dna2](https://finkelsteinlab.org/wiki/protocols/protein-purification/dna2-purification/), [BLM](https://finkelsteinlab.org/wiki/protocols/protein-purification/blm-purification/), [MRN](https://finkelsteinlab.org/wiki/protocols/protein-purification/mrn-purification/), [Rad51](https://finkelsteinlab.org/wiki/protocols/protein-purification/rad51-human-purification-protocol/), [the T4 replisome](https://finkelsteinlab.org/wiki/protocols/protein-purification/t4-replisome-purification/)), [human](https://finkelsteinlab.org/wiki/protocols/protein-purification/human-histone-purification/) and [yeast](https://finkelsteinlab.org/wiki/protocols/protein-purification/yeast-histone-purification/) histones, and the workhorse proteases ([TEV](https://finkelsteinlab.org/wiki/protocols/protein-purification/tev-protease-purification/), [SUMO](https://finkelsteinlab.org/wiki/protocols/protein-purification/sumo-protease-purification-protocol/), [sortase](https://finkelsteinlab.org/wiki/protocols/protein-purification/sortase-purification/)). - [Single-molecule imaging](https://finkelsteinlab.org/wiki/protocols/single-molecule/). Building [DNA curtains](https://finkelsteinlab.org/wiki/protocols/single-molecule/preparing-dna-curtains/) and [ssDNA curtains](https://finkelsteinlab.org/wiki/protocols/single-molecule/preparing-ssdna-curtains/), [flowcells](https://finkelsteinlab.org/wiki/protocols/single-molecule/preparing-flowcells-for-single-molecule-experiments/) and their [troubleshooting](https://finkelsteinlab.org/wiki/protocols/single-molecule/troubleshooting-flowcells/), and [particle tracking](https://finkelsteinlab.org/wiki/protocols/single-molecule/particle-tracking/). - [Microfabrication](https://finkelsteinlab.org/wiki/protocols/microfabrication/). [UV](https://finkelsteinlab.org/wiki/protocols/microfabrication/updated-for-cnm-uv-mask-lithography-and-curtain-track-fabrication/) and [e-beam](https://finkelsteinlab.org/wiki/protocols/microfabrication/pmma-for-electron-beam-lithography-ebl/) lithography for curtain tracks, [slide fabrication](https://finkelsteinlab.org/wiki/protocols/microfabrication/slide-nanofabrication/) and [dicing](https://finkelsteinlab.org/wiki/protocols/microfabrication/disco-dicing-saw-protocol/). - [Biochemical assays](https://finkelsteinlab.org/wiki/protocols/assays/). [EMSAs](https://finkelsteinlab.org/wiki/protocols/assays/cas12a-emsa-protocol/), [cleavage reactions](https://finkelsteinlab.org/wiki/protocols/assays/cas12a-cpf1-cleavage-reaction/), [ATPase](https://finkelsteinlab.org/wiki/protocols/assays/atpase-assay/) and [filter-binding](https://finkelsteinlab.org/wiki/protocols/assays/filter-binding-assay-protocol/) assays, plus [NucleaSeq](https://finkelsteinlab.org/wiki/protocols/assays/nucleaseq/) and [CHAMP](https://finkelsteinlab.org/wiki/protocols/assays/champ/). - [Protein labeling](https://finkelsteinlab.org/wiki/protocols/protein-labeling/). [Sortase reactions](https://finkelsteinlab.org/wiki/protocols/protein-labeling/sortase-optimization/), [biotinylation checks](https://finkelsteinlab.org/wiki/protocols/protein-labeling/analyzing-protein-biotinylation-via-streptavidin-gel-shift/), [epitope tags](https://finkelsteinlab.org/wiki/protocols/protein-labeling/epitope-tags-for-labeling-proteins/) and [quantum-dot conjugation](https://finkelsteinlab.org/wiki/protocols/protein-labeling/quantum-dot-antibody-conjugation/). - [DNA, RNA and cloning](https://finkelsteinlab.org/wiki/protocols/dna-and-cloning/) and [fission yeast](https://finkelsteinlab.org/wiki/protocols/yeast/). The everyday molecular biology, including [crRNA transcription](https://finkelsteinlab.org/wiki/protocols/dna-and-cloning/cas12a-cpf1-crrna-transcription/) and [*S. pombe* genetics](https://finkelsteinlab.org/wiki/protocols/yeast/s-pombe-transformation-by-lithium-acetate/). - [Recipes and buffers](https://finkelsteinlab.org/wiki/recipes/). [Media](https://finkelsteinlab.org/wiki/recipes/bacterial-media-recipes/), [antibiotics](https://finkelsteinlab.org/wiki/recipes/bacterial-antibiotics/), [gel buffers](https://finkelsteinlab.org/wiki/recipes/electrophoresis-buffers/), [loading dyes](https://finkelsteinlab.org/wiki/recipes/gel-loading-dyes/), [imaging buffers](https://finkelsteinlab.org/wiki/recipes/microscope-buffers/). - [Computing and code](https://finkelsteinlab.org/wiki/computing/). [Fitting data to a model](https://finkelsteinlab.org/wiki/computing/fitting-data-to-a-model/), [an introduction to diffusion](https://finkelsteinlab.org/wiki/computing/introduction-to-diffusion/), [ImageJ macros](https://finkelsteinlab.org/wiki/computing/imagej-macros/) and [an ND2 file reader](https://finkelsteinlab.org/wiki/computing/nd2reader/). - [Careers and writing](https://finkelsteinlab.org/wiki/careers/). [Writing advice](https://finkelsteinlab.org/wiki/careers/writing-advice/), [funding](https://finkelsteinlab.org/wiki/careers/get-funded/), and [Heilmeier's catechism](https://finkelsteinlab.org/wiki/careers/heilmeier-s-catechism/) for anyone pitching a project. Future writing advice will be on my [blog](https://finkelsteinlab.org/blog/). The [home page](https://finkelsteinlab.org/wiki/) links every section, and the search box at the top covers the whole site. ## Pull requests welcome If you find a mistake or want to help us improve this resource, open a pull request or an issue on [finkelsteinlab/wiki](https://github.com/finkelsteinlab/wiki) (every page has an **Edit page** link). And if a protocol from one of [our papers](https://finkelsteinlab.org/papers/) is not on the wiki yet, ask, and we'll try to add it in due time.