FastQCFastQC Report
Tue 21 Nov 2017
IMR90-H3K4me3-1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameIMR90-H3K4me3-1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403687
Sequences flagged as poor quality0
Sequence length36
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA3546306.562741328281968Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGACA256910.4754346430501989Illumina Single End Adapter 1 (100% over 33bp)
AATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA205500.38029589796744334Illumina Single End Adapter 1 (96% over 33bp)
CATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA195020.36090173246525936Illumina Single End Adapter 1 (96% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAC164810.30499545958157825Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTTAAA142370.26346825787652023Illumina Single End Adapter 1 (96% over 32bp)
TATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA137990.25536268107312654Illumina Single End Adapter 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT633300.029.62461712
TCGTATG637250.029.62102913
CGTATGC625500.029.6105714
TATGCCG617350.029.59380116
GTATGCC620450.029.5447315
ATGCCGT612700.029.5402917
GCTCGTA632100.029.53828611
AGCTCGT634400.029.44491610
CTTGAAA484300.029.40311230
TGCTTGA539300.029.3490228
CCGTCTT620450.029.33520320
TGCCGTC617500.029.30932418
CGTCTTC610700.029.26400221
GCCGTCT618750.029.255519
GCTTGAA502250.029.23603229
GTCTTCT615850.029.20525622
AAGAGCT629900.029.0989277
GAGCTCG640950.029.0892269
TCTGCTT587900.029.08675426
AGAGCTC632750.029.072938
Produced by FastQC (version 0.11.2)