FastQCFastQC Report
Tue 21 Nov 2017
IMR90-H3K4me3-1-1_trimmed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameIMR90-H3K4me3-1-1_trimmed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4674234
Sequences flagged as poor quality0
Sequence length20-36
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA142630.30514090651002923Illumina Single End Adapter 1 (96% over 33bp)
TATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA113640.24312004918880825Illumina Single End Adapter 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA52800.033.6903630
GCTTGAA58700.029.71758829
TGCTTGA61700.029.21015728
ATCGGAA75000.027.8169542
TATCGGA23000.027.674391
TCGTATG110850.027.42112213
CTCGTAT106750.027.29968312
TATGCCG104350.027.26088716
CGTATGC107550.027.24402614
ATGCCGT101200.027.10813317
GTATGCC106600.027.09825315
GCTCGTA103900.027.03646511
CATCGGA32350.026.9035971
CCGTCTT104650.026.65355720
AGCTCGT103250.026.4950310
TCGGAAG87550.026.4737833
CTGCTTG75000.026.40137927
GATCGGA27500.026.189981
ATCGTAT6500.026.16453212
GCCGTCT106750.026.1630219
Produced by FastQC (version 0.11.2)