##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ecoli_ref-5m.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5000000
Sequences flagged as poor quality	0
Sequence length	100
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.6172826	39.0	38.0	39.0	35.0	39.0
2	37.162367	39.0	37.0	39.0	34.0	39.0
3	37.1660638	39.0	37.0	39.0	34.0	39.0
4	37.18766	39.0	37.0	39.0	34.0	39.0
5	37.1768096	39.0	37.0	39.0	34.0	39.0
6	37.2609776	39.0	37.0	39.0	34.0	39.0
7	37.2543786	39.0	37.0	39.0	34.0	39.0
8	37.2302516	39.0	37.0	39.0	34.0	39.0
9	37.209263	39.0	37.0	39.0	34.0	39.0
10-11	37.1848397	39.0	37.0	39.0	33.5	39.0
12-13	37.136373	39.0	37.0	39.0	33.0	39.0
14-15	37.0882342	39.0	37.0	39.0	33.0	39.0
16-17	37.0440371	39.0	37.0	39.0	33.0	39.0
18-19	36.9783678	39.0	37.0	39.0	33.0	39.0
20-21	36.8932461	39.0	37.0	39.0	33.0	39.0
22-23	36.7878836	39.0	37.0	39.0	33.0	39.0
24-25	36.651882799999996	39.0	36.5	39.0	32.5	39.0
26-27	36.5465326	39.0	36.0	39.0	32.0	39.0
28-29	36.402545599999996	39.0	36.0	39.0	32.0	39.0
30-31	36.278822	39.0	36.0	39.0	31.5	39.0
32-33	36.1759199	39.0	36.0	39.0	31.0	39.0
34-35	36.0749252	39.0	36.0	39.0	31.0	39.0
36-37	35.8471387	38.5	36.0	39.0	30.5	39.0
38-39	35.6974099	38.0	36.0	39.0	30.0	39.0
40-41	35.5637185	38.0	36.0	39.0	30.0	39.0
42-43	35.3503447	38.0	35.0	39.0	29.0	39.0
44-45	35.0437208	38.0	35.0	39.0	28.0	39.0
46-47	34.9394991	38.0	35.0	39.0	28.0	39.0
48-49	34.6596508	38.0	34.5	39.0	27.5	39.0
50-51	34.409406000000004	37.5	34.0	39.0	26.0	39.0
52-53	34.1046646	37.0	33.5	39.0	25.0	39.0
54-55	33.840446099999994	37.0	33.0	39.0	24.5	39.0
56-57	33.5543167	37.0	33.0	39.0	23.0	39.0
58-59	33.3046863	37.0	33.0	39.0	22.5	39.0
60-61	32.9505985	37.0	32.0	39.0	20.5	39.0
62-63	32.5124566	36.5	31.5	39.0	19.5	39.0
64-65	31.9681773	36.0	31.0	38.5	17.0	39.0
66-67	31.5929738	36.0	30.0	38.0	13.5	39.0
68-69	31.0688074	36.0	29.5	38.0	11.0	39.0
70-71	30.5110654	36.0	28.5	38.0	4.0	39.0
72-73	30.0128964	35.0	28.0	38.0	2.0	39.0
74-75	29.534274699999997	35.0	27.0	38.0	2.0	39.0
76-77	28.8659933	34.5	25.5	38.0	2.0	39.0
78-79	28.173423	34.0	24.0	37.0	2.0	39.0
80-81	27.5444274	33.5	22.0	37.0	2.0	39.0
82-83	26.9764373	33.0	20.5	37.0	2.0	39.0
84-85	26.1496475	32.5	18.5	37.0	2.0	38.5
86-87	25.417372999999998	32.0	14.5	36.5	2.0	38.0
88-89	24.8173625	31.5	11.5	36.0	2.0	38.0
90-91	23.7760787	30.5	2.0	36.0	2.0	38.0
92-93	22.8238884	29.5	2.0	36.0	2.0	38.0
94-95	21.9174362	28.0	2.0	35.0	2.0	38.0
96-97	20.642442	26.5	2.0	35.0	2.0	38.0
98-99	19.5973389	24.5	2.0	34.5	2.0	37.5
100	18.233471	21.0	2.0	33.0	2.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16327.0
3	3453.0
4	5472.0
5	6130.0
6	7009.0
7	7171.0
8	8554.0
9	9026.0
10	11619.0
11	13403.0
12	16440.0
13	17756.0
14	21023.0
15	23736.0
16	27053.0
17	31500.0
18	36370.0
19	42036.0
20	48132.0
21	55032.0
22	62481.0
23	71150.0
24	81432.0
25	91575.0
26	103490.0
27	117387.0
28	134022.0
29	154529.0
30	180336.0
31	216576.0
32	264430.0
33	333208.0
34	430722.0
35	562455.0
36	716359.0
37	772616.0
38	299987.0
39	3.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	24.24060892240468	26.838912505846395	24.794995643231953	24.12548292851697
2	25.06735257913928	26.661670616256078	25.269230122379877	23.001746682224766
3	24.114423167984548	25.463743912569655	25.54861705235325	24.873215867092547
4	24.838061778575586	24.973582557560796	25.3263890529482	24.86196661091542
5	25.43023761921904	25.1648014594384	25.321564555763814	24.08339636557875
6	25.128612831332198	24.881517631409718	24.60479182900099	25.385077708257086
7	25.52218702966326	24.929406316280126	25.019706965383616	24.528699688672994
8	24.889714892574137	24.977554537867757	25.039725064112726	25.093005505445376
9	25.103456422567106	24.43813987664715	25.113807570753703	25.344596130032045
10-11	25.246044538370814	24.747729146900788	24.80983213174802	25.19639418298038
12-13	25.236623410142272	24.55131880489973	24.791063288358842	25.420994496599157
14-15	25.220408201264267	24.549505967896597	24.827536982960062	25.402548847879075
16-17	25.27921442017967	24.55354318855341	24.76998040614608	25.397261985120846
18-19	25.3702545557626	24.445679182385053	24.698070872667664	25.485995389184684
20-21	25.35266457288054	24.467671442459434	24.728352118789918	25.451311865870107
22-23	25.35365839011618	24.590202656716656	24.66379683083649	25.392342122330675
24-25	25.394915753708226	24.527258225835	24.626695356023763	25.45113066443302
26-27	25.333353490157428	24.569633450823588	24.675677034015443	25.421336025003537
28-29	25.343921261603846	24.652419016256676	24.676019467919456	25.327640254220018
30-31	25.34085500139151	24.64643162906083	24.649666302840973	25.363047066706685
32-33	25.287976073222428	24.624381538566308	24.71178858470787	25.37585380350339
34-35	25.29350590607551	24.754455635749093	24.67184379551326	25.280194662662144
36-37	25.29419223229949	24.655800897139084	24.69330855390907	25.35669831665236
38-39	25.2725873935103	24.63472578219968	24.738338735641555	25.354348088648464
40-41	25.27727551091431	24.74055572775339	24.724612266950817	25.257556494381483
42-43	25.282036059595114	24.67175577411798	24.679306913558882	25.366901252728024
44-45	25.265968074717136	24.652442509582865	24.770802997490872	25.310786418209126
46-47	25.267213741344953	24.72601526798299	24.75380036875857	25.25297062191349
48-49	25.338415242843432	24.62580656213069	24.683548551807057	25.352229643218816
50-51	25.31022119527726	24.58439930681215	24.755562736335097	25.349816761575493
52-53	25.312304811058358	24.69463245750109	24.715509378335586	25.277553353104963
54-55	25.35609390478791	24.54723103411211	24.70722247505587	25.389452586044108
56-57	25.347231997169185	24.551236011362125	24.71985655982623	25.38167543164246
58-59	25.364549016323494	24.5792347438502	24.706974790900933	25.34924144892537
60-61	25.40814853339444	24.490655296921414	24.67471300032892	25.42648316935523
62-63	25.394264690766526	24.45548103508144	24.70793185881508	25.442322415336953
64-65	25.44410350415504	24.532431663937118	24.68421165478764	25.33925317712021
66-67	25.470908373871442	24.39845445148564	24.65276696077018	25.477870213872734
68-69	25.469761106019707	24.359867991480904	24.685801469631258	25.48456943286813
70-71	25.48670718347086	24.40724107859232	24.65163053732914	25.45442120060768
72-73	25.57371790119276	24.296768769163503	24.59597091014598	25.533542419497753
74-75	25.557965189441518	24.248475808030676	24.64131560799432	25.55224339453349
76-77	25.590856602791973	24.273859420097583	24.634018814903943	25.5012651622065
78-79	25.6850498325554	24.12226361852693	24.59974627783979	25.59294027107788
80-81	25.706228587879515	24.05612812646144	24.653064651940344	25.584578633718703
82-83	25.710064552006074	24.029688411950513	24.577185612692972	25.683061423350438
84-85	25.74527048554545	23.927886551767667	24.551254691441887	25.77558827124499
86-87	25.82043580601152	23.869054840040075	24.600106446611555	25.710402907336853
88-89	25.841445868328787	23.847182026104676	24.544905942336943	25.766466163229595
90-91	25.95587227216068	23.610073914079326	24.551177290540807	25.88287652321919
92-93	25.96534758561463	23.579461702134477	24.60060660050566	25.854584111745233
94-95	26.036306388544357	23.578281863480246	24.57295452963583	25.812457218339567
96-97	26.15094716020185	23.402101542367536	24.513280752712856	25.933670544717764
98-99	26.201902180553326	23.24624953881616	24.61448836334517	25.937359917285352
100	26.084654823513436	23.328578899728853	24.600478354374424	25.98628792238329
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	72.0
1	64.5
2	53.0
3	53.5
4	61.0
5	64.0
6	69.0
7	81.5
8	96.0
9	118.5
10	167.0
11	216.5
12	258.5
13	309.5
14	349.5
15	389.5
16	432.0
17	496.5
18	576.0
19	761.0
20	1040.0
21	1412.5
22	1876.5
23	2614.0
24	3590.0
25	4593.5
26	6014.5
27	7826.5
28	10070.0
29	12693.5
30	15429.5
31	18404.5
32	21510.0
33	25077.0
34	29527.0
35	34535.0
36	39806.0
37	45017.5
38	51142.5
39	59503.5
40	69580.5
41	80677.0
42	93804.0
43	108675.0
44	124888.0
45	143399.0
46	163545.0
47	185975.5
48	210165.5
49	233966.5
50	255884.5
51	275233.5
52	289020.0
53	295114.0
54	292013.0
55	279389.5
56	259439.5
57	233454.0
58	204269.0
59	173893.0
60	144203.5
61	116953.0
62	92600.5
63	71624.0
64	54532.5
65	40904.0
66	30464.0
67	22484.5
68	16361.0
69	11908.0
70	8599.5
71	6126.0
72	4335.0
73	3064.0
74	2177.0
75	1512.5
76	1038.0
77	733.5
78	490.0
79	335.5
80	229.5
81	159.0
82	104.5
83	59.5
84	48.5
85	38.0
86	24.5
87	18.0
88	14.5
89	10.0
90	8.0
91	8.5
92	7.0
93	7.0
94	7.0
95	4.0
96	4.5
97	6.0
98	10.0
99	15.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10944
2	0.10776
3	0.10973999999999999
4	0.10388000000000001
5	0.10404
6	0.10328
7	0.11146
8	0.11344
9	0.1077
10-11	0.10142000000000001
12-13	0.11449
14-15	0.10467
16-17	0.09112
18-19	0.09576
20-21	0.08849
22-23	0.0872
24-25	0.09768000000000002
26-27	0.11654
28-29	0.12903
30-31	0.14449
32-33	0.14534999999999998
34-35	0.15958999999999998
36-37	0.15372
38-39	0.14762
40-41	0.14715
42-43	0.14752
44-45	0.15249000000000001
46-47	0.16232
48-49	0.17662999999999998
50-51	0.16559000000000001
52-53	0.17685
54-55	0.17591
56-57	0.18417
58-59	0.18009
60-61	0.18891
62-63	0.18254
64-65	0.19105999999999998
66-67	0.17007
68-69	0.19129
70-71	0.20437
72-73	0.18788
74-75	0.20614000000000002
76-77	0.20252
78-79	0.20579999999999998
80-81	0.20547
82-83	0.19675
84-85	0.1906
86-87	0.17531
88-89	0.15965000000000001
90-91	0.14487
92-93	0.13856000000000002
94-95	0.14705000000000001
96-97	0.14572000000000002
98-99	0.14828
100	0.14934
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5000000.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.04863905039228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.52089030722777	62.06969515811493
2	17.5424179873945	27.734085351133142
3	3.2297009130805567	7.659103851564819
4	0.5106749251830695	1.6147263133153016
5	0.1087287384183288	0.42974293988174966
6	0.043221362375859815	0.20499539242293285
7	0.019471895313910973	0.10774587770074615
8	0.011808961397501112	0.07467858616568651
9	0.006511967866169161	0.04632859776245033
>10	0.00657294174230467	0.058897931938218066
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.0E-5	0.0
2	0.0	0.0	0.0	2.0E-5	0.0
3	0.0	0.0	0.0	2.0E-5	0.0
4	0.0	0.0	0.0	2.0E-5	0.0
5	0.0	0.0	0.0	4.0E-5	0.0
6	4.0E-5	0.0	0.0	4.0E-5	0.0
7	4.0E-5	0.0	0.0	4.0E-5	0.0
8	4.0E-5	0.0	0.0	4.0E-5	0.0
9	4.0E-5	0.0	0.0	4.0E-5	0.0
10-11	4.0E-5	0.0	0.0	6.000000000000001E-5	0.0
12-13	4.0E-5	0.0	0.0	1.1E-4	0.0
14-15	4.0E-5	0.0	0.0	1.6E-4	0.0
16-17	4.0E-5	0.0	0.0	1.8E-4	0.0
18-19	4.0E-5	0.0	0.0	2.2E-4	0.0
20-21	8.0E-5	0.0	0.0	2.4E-4	0.0
22-23	1.0E-4	0.0	0.0	2.6999999999999995E-4	0.0
24-25	1.2E-4	0.0	0.0	3.0E-4	0.0
26-27	1.2E-4	0.0	0.0	3.0E-4	0.0
28-29	2.8000000000000003E-4	0.0	0.0	3.0E-4	0.0
30-31	3.2E-4	0.0	0.0	3.2E-4	0.0
32-33	3.6E-4	0.0	0.0	3.3E-4	0.0
34-35	3.8E-4	0.0	0.0	3.8E-4	0.0
36-37	4.6E-4	0.0	0.0	4.2E-4	0.0
38-39	5.2E-4	0.0	0.0	4.5E-4	0.0
40-41	5.8E-4	0.0	0.0	4.7000000000000004E-4	0.0
42-43	6.6E-4	0.0	0.0	5.1E-4	2.0E-5
44-45	8.4E-4	0.0	0.0	5.2E-4	2.0E-5
46-47	0.00106	0.0	0.0	5.2E-4	2.0E-5
48-49	0.0012000000000000001	0.0	0.0	5.3E-4	2.0E-5
50-51	0.00146	0.0	0.0	5.4E-4	2.0E-5
52-53	0.00164	0.0	0.0	5.4E-4	2.0E-5
54-55	0.0019500000000000001	0.0	0.0	5.6E-4	2.0E-5
56-57	0.00224	0.0	0.0	5.8E-4	4.0E-5
58-59	0.0024200000000000003	0.0	0.0	6.4E-4	4.0E-5
60-61	0.0026	0.0	0.0	6.7E-4	4.0E-5
62-63	0.0034000000000000002	0.0	0.0	7.1E-4	4.0E-5
64-65	0.0038599999999999997	0.0	0.0	7.3E-4	4.0E-5
66-67	0.00438	0.0	0.0	7.5E-4	4.0E-5
68-69	0.004739999999999999	1.0E-5	0.0	7.9E-4	4.0E-5
70-71	0.0053	2.0E-5	1.0E-5	8.1E-4	4.0E-5
72-73	0.00583	2.0E-5	2.0E-5	8.2E-4	4.0E-5
74-75	0.00632	2.0E-5	2.0E-5	8.4E-4	4.0E-5
76-77	0.007520000000000001	2.0E-5	2.0E-5	8.4E-4	5.0E-5
78-79	0.00792	2.0E-5	2.0E-5	8.500000000000001E-4	6.0E-5
80-81	0.00852	4.0E-5	2.0E-5	9.1E-4	7.000000000000001E-5
82-83	0.00896	4.0E-5	4.0E-5	9.599999999999999E-4	8.0E-5
84-85	0.00932	4.0E-5	5.0E-5	0.00103	8.0E-5
86-87	0.00979	4.0E-5	7.000000000000001E-5	0.0010999999999999998	9.0E-5
88	0.01044	4.0E-5	8.0E-5	0.00112	1.2E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCTAG	355	0.009657256	6.6209736	92-93
>>END_MODULE
