FastQCFastQC Report
Sun 19 Aug 2018
ecoli_ref-5m.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameecoli_ref-5m.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5000000
Sequences flagged as poor quality0
Sequence length100
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG3550.0096572566.620973692-93
Produced by FastQC (version 0.11.5)