,id,gene_name_short,gene_name_long,product,plasmid_name,backbone,sequencing_primer_forward,sequencing_primer_reverse,genbank_source_id,genbank_source_link,genbank_protein_id,genbank_protein_link,locus_tag,uniprot_link,origin_organism,target_organism,description,shipped as,growth_conditions,growth_media,growth_antibotic,expression_strain,expression_promoter,expression_inducer,cloning_method,cloning_enzyme,cloning_part_type,cloning_prefix,cloning_suffix,production_sequence,plasmid_size,insert_sequence,construct_insert_size,ori,copy_number,bsl,export_controls,safety_other_information,genbank_file_link,attribution,citations,protocols_and_instructions,canary_notice,documentation_image,trading_card_link,available_elsewhere,internal_notes_1,internal_notes_2,row-hash,changed
2135,BBF10K_000034,Sc-pAdh1,S. cerevisiae promoter Adh,Open Yeast Collection,pOpen-Sc-pAdh1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/ADH1,,S. cerevisiae,yeast,S. cerevisiae Adh promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGACTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGTCACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTAATGTGAGACCACATAAGCGATCCCAAGGTCAGGGCATGGCGCAAATACAGATAGGGATAACAGGGTAATCTGCTTCTTATGGACCAAAGT,2828,AACGATGATGCTCACTCTCGGGTCTCTGGAGATCCTTTTGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTATAATACCTTCGTTGGACTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACAAGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAATACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTATTGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAATGATGGAAGTCACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAAGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTAATGTGAGACCACATAAGCGATCCCAAGGTCAGGGCATGGCGCAAATACAGATAGGGATAACAGGGTAATCTGCTTCTTATGGACCAAAGT,827,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000034.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/8401308 https://www.ncbi.nlm.nih.gov/pubmed/7737504,,we are unaware,freegenes.github.io/genes/images/BBF10K_000034.png,freegenes.github.io/genes/BBF10K_000034.html,,,,1f736e37,False
2136,BBF10K_000037,Sc-pCCW12,S. cerevisiae promoter CCW12 strong,Open Yeast Collection,pOpen-Sc-pCCW12,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000004100,,S. cerevisiae,yeast,S. cerevisiae CCW12 strong promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGCACCCATGAACCACACGGTTAGTCCAAAAGGGGCAGTTCAGATTCCAGATGCGGGAATTAGCTTGCTGCCACCCTCACCTCACTAACGCTGCGGTGTGCGGATACTTCATGCTATTTATAGACGCGCGTGTCGGAATCAGCACGCGCAAGAACCAAATGGGAAAATCGGAATGGGTCCAGAACTGCTTTGAGTGCTGGCTATTGGCGTCTGATTTCCGTTTTGGGAATCCTTTGCCGCGCGCCCCTCTCAAAACTCCGCACAAGTCCCAGAAAGCGGGAAAGAAATAAAACGCCACCAAATAAAATAAAATAAAAGCCAATCCTCGAAGCGTGGGTGGTAGGCCCTGGATTATCCCGTACAAGTATTTCTCAGGAGTAAAAAAACCGTTTGTTTTGGAATTTCCCATTTCGCGGCCACCTACGCCGCTATCTTTGCAACAACTATCTGCGATAACTCAGCAAATTTTGCATATTCGTGTTGCAGTATTGCGATAATGGGAGTCTTACTTCCAACATAACGGCAGAAAGAAATGTGAGAAAATTTTGCATCCTTTGCCTCCGTTCAAGTATATAAAGTCGGCATGCTTGATAATCTTTCTTTCCATCCTACATTGTTCTAATTATTCTTATTCTCCTTTATTCTTTCCTAACATACCAAGAAATTAATCTTCTGTCATTCGCTTAAACACTATATCAATAAAATGTGAGACCACATTAAATTTCGCCGTGGCAGAAGTAGATCAATGTTCGAGTAGGGATAACAGGGTAATCGGAGAGAGCCGTGTCCGAAG,2823,AACGATGATGCTCACTCTCGGGTCTCTGGAGCACCCATGAACCACACGGTTAGTCCAAAAGGGGCAGTTCAGATTCCAGATGCGGGAATTAGCTTGCTGCCACCCTCACCTCACTAACGCTGCGGTGTGCGGATACTTCATGCTATTTATAGACGCGCGTGTCGGAATCAGCACGCGCAAGAACCAAATGGGAAAATCGGAATGGGTCCAGAACTGCTTTGAGTGCTGGCTATTGGCGTCTGATTTCCGTTTTGGGAATCCTTTGCCGCGCGCCCCTCTCAAAACTCCGCACAAGTCCCAGAAAGCGGGAAAGAAATAAAACGCCACCAAATAAAATAAAATAAAAGCCAATCCTCGAAGCGTGGGTGGTAGGCCCTGGATTATCCCGTACAAGTATTTCTCAGGAGTAAAAAAACCGTTTGTTTTGGAATTTCCCATTTCGCGGCCACCTACGCCGCTATCTTTGCAACAACTATCTGCGATAACTCAGCAAATTTTGCATATTCGTGTTGCAGTATTGCGATAATGGGAGTCTTACTTCCAACATAACGGCAGAAAGAAATGTGAGAAAATTTTGCATCCTTTGCCTCCGTTCAAGTATATAAAGTCGGCATGCTTGATAATCTTTCTTTCCATCCTACATTGTTCTAATTATTCTTATTCTCCTTTATTCTTTCCTAACATACCAAGAAATTAATCTTCTGTCATTCGCTTAAACACTATATCAATAAAATGTGAGACCACATTAAATTTCGCCGTGGCAGAAGTAGATCAATGTTCGAGTAGGGATAACAGGGTAATCGGAGAGAGCCGTGTCCGAAG,822,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000037.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000037.png,,,,,07edeeb2,False
2137,BBF10K_000039,Sc-pCUP1,S. cerevisiae promoter CUP1 inducible,Open Yeast Collection,pOpen-Sc-pCUP1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000001095,,S. cerevisiae,yeast,S. cerevisiae CUP1 inducible promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTTCTTAGCCTTGTTGCTAGTTAGAAAAAGACATTTTTGCTGTCAGTCACTGTCAAGAGATTCTTTTGCTGGCATTTCTTCTAGGAGCAAAAAGAGCGAAGCGTCTTTTCCGCTGAACCGTTCCAGCAAAAAAGACTACCAACGCAATATGGATTGTCAGAATCATATAAAAGAGAAGCAAATAACTCCTTGTCTTGTATCAATTGCATTATAATATCTTCTTGTTAGTGCAATATCATATAGAAGTCATCGAAATAGATATTAAGAAAAACAAACTGTACAATCAATCAATCAATCATCACATAAAAATGTGAGACCACCACAGGTCAAGATTCACGGAATCGACTTTCATTTTCGCCTAGGGATAACAGGGTAATAGTTAACTGTAGCTACTGTAG,2429,AACGATGATGCTCACTCTCGGGTCTCTGGAGTTCTTAGCCTTGTTGCTAGTTAGAAAAAGACATTTTTGCTGTCAGTCACTGTCAAGAGATTCTTTTGCTGGCATTTCTTCTAGGAGCAAAAAGAGCGAAGCGTCTTTTCCGCTGAACCGTTCCAGCAAAAAAGACTACCAACGCAATATGGATTGTCAGAATCATATAAAAGAGAAGCAAATAACTCCTTGTCTTGTATCAATTGCATTATAATATCTTCTTGTTAGTGCAATATCATATAGAAGTCATCGAAATAGATATTAAGAAAAACAAACTGTACAATCAATCAATCAATCATCACATAAAAATGTGAGACCACCACAGGTCAAGATTCACGGAATCGACTTTCATTTTCGCCTAGGGATAACAGGGTAATAGTTAACTGTAGCTACTGTAG,428,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000039.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000039.png,,,,,ec98d354,False
2138,BBF10K_000046,Sc-pCYC1,S. cerevisiae promoter CYC1,Open Yeast Collection,pOpen-Sc-pCYC1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003809,,S. cerevisiae,yeast,S. cerevisiae CYC1 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGTGCAGATCCGCCAGGCGTGTATATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACATTAGGACCTTTGCAGCATAAATTACTATACTTCTATAGACACACAAACACAAATACACACACTAAATTAATAAATGTGAGACCACCCGTATCGCATAAGGATGTAGTATGCTGGCTTCCATTAGTAGGGATAACAGGGTAATCACCATACCTTCTTATATGGA,2411,AACGATGATGCTCACTCTCGGGTCTCTGGAGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGTGCAGATCCGCCAGGCGTGTATATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACATTAGGACCTTTGCAGCATAAATTACTATACTTCTATAGACACACAAACACAAATACACACACTAAATTAATAAATGTGAGACCACCCGTATCGCATAAGGATGTAGTATGCTGGCTTCCATTAGTAGGGATAACAGGGTAATCACCATACCTTCTTATATGGA,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000046.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/7737504,,we are unaware,freegenes.github.io/genes/images/BBF10K_000046.png,,,,,ecae18bd,False
2140,BBF10K_000068,Sc-pHis3,S. cerevisiae promoter His3,Open Yeast Collection,pOpen-Sc-pHis3,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000005728,,S. cerevisiae,yeast,S. cerevisiae His3 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATTACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTTTTATTTTTCCACCTAGCGGATGACTCTTATTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATAAACGAAGGCAAAGAATGTGAGACCCTCGAGACGATGGGGACATAGAACACTCCCACTGAGATGGGGTGGCTTAGGGATAACAGGGTAATTCTGCATTGCCAAGGGAGATA,2366,AACGATGATGCTCACTCTCGGGTCTCTGGAGAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATTACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTTTTATTTTTCCACCTAGCGGATGACTCTTATTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATAAACGAAGGCAAAGAATGTGAGACCCTCGAGACGATGGGGACATAGAACACTCCCACTGAGATGGGGTGGCTTAGGGATAACAGGGTAATTCTGCATTGCCAAGGGAGATA,365,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000068.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000068.png,,,,,a9248c4b,False
2141,BBF10K_000078,Sc-pLeu2,S. cerevisiae promoter Leu2,Open Yeast Collection,pOpen-Sc-pLeu2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000523,,S. cerevisiae,yeast,S. cerevisiae Leu2 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATTTCAAGGATATACCATTCTAAATGTGAGACCACGGAGCCCTTATTGTAACCTATACACTTGGGACACCGCGATAGGGATAACAGGGTAATTCAGGAATGTATACCTACGAA,2529,AACGATGATGCTCACTCTCGGGTCTCTGGAGAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATTTCAAGGATATACCATTCTAAATGTGAGACCACGGAGCCCTTATTGTAACCTATACACTTGGGACACCGCGATAGGGATAACAGGGTAATTCAGGAATGTATACCTACGAA,528,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000078.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000078.png,,,,,b552b21b,False
2142,BBF10K_000079,Sc-pMF2,S. cerevisiae promoter MF2,Open Yeast Collection,pOpen-Sc-pMF2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003057,,S. cerevisiae,yeast,S. cerevisiae MF2 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTTGATCTGCCTTTTATAGCTAAGAAAAAAAAAGAGAAAAAAATAATAAGTGCGGGTAACCTCATGCATTGTAAAACGGTAGGGCTACAAGGCACTACTTACTCTACGCTATAGAAGATGCTTCAAGACATGGTGTTGTTCTTATTTGAGCGAACTCAGCTGTCACCGTTTCGGGAAACTCTATAGTTTTCTGCGTTTCAGTACGCAGTTGGGCGTGCTAAAGTTGTTTTCCTAATTTGCTAGTGTTCACTTGCTCATTGATGTCCCTAAAAGGTAATGTCGCATAGTAAGATGTATGTCCCATATCAAACGCATTCCTAGAAAAACATGCACTGAATGAATAAGCAGTTACTTCGATTACTAATCTTCACAGCATAATTTTTCTCTTATCTTGTAAACCTCTTTCTATCTTTTCATAAAGTTTGTCACTTATATTTCTTCATTGGTACATCAATGCCAGCAACGATGTGCGCATCTGGGCGACGCCTGTAGTGATTGTTTTCAAGGTATCGAGCCAAACTATTCATCGTTACTGTTTCAAATATTCAGTTGTTTCAGTACAGAGTCGCCGTGGACCTAGTGAAACTTGGTGTCTTTACAGCGCAGAGATGAGGGCTTATATGTATAAAAGCTGTCCTTGATTCTGGTGTAGTTTGAGGTGTCCTTCCTATATCTGTTTTTATATTCTATATAATGGATAATTACTACCATCACCTGCATCAAATTCCAGTAAATTCACATATTGGAGAAAAATGTGAGACCACGTATGGGGAACACTACACAGTGCCTGACGCCCTAAGACATAGGGATAACAGGGTAATGCCTCTTGTGGTAGAACCCAA,2873,AACGATGATGCTCACTCTCGGGTCTCTGGAGTTGATCTGCCTTTTATAGCTAAGAAAAAAAAAGAGAAAAAAATAATAAGTGCGGGTAACCTCATGCATTGTAAAACGGTAGGGCTACAAGGCACTACTTACTCTACGCTATAGAAGATGCTTCAAGACATGGTGTTGTTCTTATTTGAGCGAACTCAGCTGTCACCGTTTCGGGAAACTCTATAGTTTTCTGCGTTTCAGTACGCAGTTGGGCGTGCTAAAGTTGTTTTCCTAATTTGCTAGTGTTCACTTGCTCATTGATGTCCCTAAAAGGTAATGTCGCATAGTAAGATGTATGTCCCATATCAAACGCATTCCTAGAAAAACATGCACTGAATGAATAAGCAGTTACTTCGATTACTAATCTTCACAGCATAATTTTTCTCTTATCTTGTAAACCTCTTTCTATCTTTTCATAAAGTTTGTCACTTATATTTCTTCATTGGTACATCAATGCCAGCAACGATGTGCGCATCTGGGCGACGCCTGTAGTGATTGTTTTCAAGGTATCGAGCCAAACTATTCATCGTTACTGTTTCAAATATTCAGTTGTTTCAGTACAGAGTCGCCGTGGACCTAGTGAAACTTGGTGTCTTTACAGCGCAGAGATGAGGGCTTATATGTATAAAAGCTGTCCTTGATTCTGGTGTAGTTTGAGGTGTCCTTCCTATATCTGTTTTTATATTCTATATAATGGATAATTACTACCATCACCTGCATCAAATTCCAGTAAATTCACATATTGGAGAAAAATGTGAGACCACGTATGGGGAACACTACACAGTGCCTGACGCCCTAAGACATAGGGATAACAGGGTAATGCCTCTTGTGGTAGAACCCAA,872,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000079.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000079.png,,,,,36a2166e,False
2143,BBF10K_000084,Sc-pMFA1,S. cerevisiae promoter MFA1,Open Yeast Collection,pOpen-Sc-pMFA1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002869,,S. cerevisiae,yeast,S. cerevisiae MFA1 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGCGCTAGAAGGAAATTGGAAGAACTAAATAGAGTTCTGAAAAACAATCCATATTTCAATTCAAATGTGAACGTACACAATTCGAGACTCAAAGATGCTGTACCGTTCACGCCGTTTAACGGTGATAGAGAAGCACACCCAAGGTTTACGTTGAAAGGTTCAGTATACAATGACCCATTCATCAAACATCTTGAGCACAGGAAAGAATTTATTGCGTCTGGGTTCAACACTAATTATGCGTACGAAAGGGTGTTGACAGAGGCATTTATGGGCTTAGGATGTGTTATATCCGAGGAGCTTTAAAACATCAGGATAGTGTGCAACGTGGCATAAGCTATGTAATCAACTACTTTTTATTTTCTATGTACGCATATACATGCATTCACGATCTGTTTCAGTGTTCAGAAAAAAGGCACCTACTGCTACGGTTGGCCCATACCTTTATTCTTTGTTCTTGTTACAAACGAGTGTGTAATTACCCAAAAAGGAAATTTACATGTTAAATGAAACCCAGTAATCAGAAAAAACAGTTAAGAAACCTAAAATGGTAGAGATAAAGATACAGATTCAGTGGTTGCTGAAAATCAAGTAAAAAAATGAAATAGAGTCATCATATATAAACCGCCAGAAATGAATTAATGAGAGGGATCTGTAACTGTTTCTCGGATAAAACCAAAATAAGTACAAAGCCATCGAATAGAAAAATGTGAGACCACGTGAAACTGTATCGAGCCCCGTGGAAGATGTTTCACCAATAGGGATAACAGGGTAATAACCCAACTGATACAGAATAG,2824,AACGATGATGCTCACTCTCGGGTCTCTGGAGCGCTAGAAGGAAATTGGAAGAACTAAATAGAGTTCTGAAAAACAATCCATATTTCAATTCAAATGTGAACGTACACAATTCGAGACTCAAAGATGCTGTACCGTTCACGCCGTTTAACGGTGATAGAGAAGCACACCCAAGGTTTACGTTGAAAGGTTCAGTATACAATGACCCATTCATCAAACATCTTGAGCACAGGAAAGAATTTATTGCGTCTGGGTTCAACACTAATTATGCGTACGAAAGGGTGTTGACAGAGGCATTTATGGGCTTAGGATGTGTTATATCCGAGGAGCTTTAAAACATCAGGATAGTGTGCAACGTGGCATAAGCTATGTAATCAACTACTTTTTATTTTCTATGTACGCATATACATGCATTCACGATCTGTTTCAGTGTTCAGAAAAAAGGCACCTACTGCTACGGTTGGCCCATACCTTTATTCTTTGTTCTTGTTACAAACGAGTGTGTAATTACCCAAAAAGGAAATTTACATGTTAAATGAAACCCAGTAATCAGAAAAAACAGTTAAGAAACCTAAAATGGTAGAGATAAAGATACAGATTCAGTGGTTGCTGAAAATCAAGTAAAAAAATGAAATAGAGTCATCATATATAAACCGCCAGAAATGAATTAATGAGAGGGATCTGTAACTGTTTCTCGGATAAAACCAAAATAAGTACAAAGCCATCGAATAGAAAAATGTGAGACCACGTGAAACTGTATCGAGCCCCGTGGAAGATGTTTCACCAATAGGGATAACAGGGTAATAACCCAACTGATACAGAATAG,823,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000084.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000084.png,,,,,c65d2f49,False
2144,BBF10K_000094,Sc-pMFalpha2,S. cerevisiae promoter MFalpha2,Open Yeast Collection,pOpen-Sc-pMFalpha2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/MF%28ALPHA%292,,S. cerevisiae,yeast,S. cerevisiae MFalpha2 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTTGATCTGCCTTTTATAGCTAAGAAAAAAAAAGAGAAAAAAATAATAAGTGCGGGTAACCTCATGCATTGTAAAACGGTAGGGCTACAAGGCACTACTTACTCTACGCTATAGAAGAAGCATCAAGACATGGTGTTGTTCTTATTTGAGCGAACTCAGCTGTCACCGTTTCGGGAAACTCTATAGTTTTCTGCGTTTCAGTACGCAGTTGGGCGTGCTAAAGTTGTTTTCCTAATTTGCTAGTGTTCACTTGCTCATTGATGTCCCTAAAAGGTAATGTCGCATAGTAAGATGTATGTCCCATATCAAACGCATTCCTAGAAAAACATGCACTGAATGAATAAGCAGTTACTTCGATTACTAATCTTCACAGCATAATTTTTCTCTTATCTTGTAAACCTCTTTCTATCTTTTCATAAAGTTTGTCACTTATATTTCTTCATTGGTACATCAATGCCAGCAACGATGTGCGCATCTGGGCGACGCCTGTAGTGATTGTTTTCAAGGTATCGAGCCAAACTATTCATCGTTACTGTTTCAAATATTCAGTTGTTTCAGTACAGAGTCGCCGTGGACCTAGTGAAACTTGGTGTCTTTACAGCGCAGAGATGAGGGCTTATATGTATAAAAGCTGTCCTTGATTCTGGTGTAGTTTGAGGTGTCCTTCCTATATCTGTTTTTATATTCTATATAATGGATAATTACTACCATCACCTGCATCAAATTCCAGTAAATTCACATATTGGAGAAAAAATGTGAGACCACGTTCAGTTTTCCAATGGCAGCCTCTTTATGCCAAGAATGTAGGGATAACAGGGTAATAATACTGGTGTATATTCAAGA,2874,AACGATGATGCTCACTCTCGGGTCTCTGGAGTTGATCTGCCTTTTATAGCTAAGAAAAAAAAAGAGAAAAAAATAATAAGTGCGGGTAACCTCATGCATTGTAAAACGGTAGGGCTACAAGGCACTACTTACTCTACGCTATAGAAGAAGCATCAAGACATGGTGTTGTTCTTATTTGAGCGAACTCAGCTGTCACCGTTTCGGGAAACTCTATAGTTTTCTGCGTTTCAGTACGCAGTTGGGCGTGCTAAAGTTGTTTTCCTAATTTGCTAGTGTTCACTTGCTCATTGATGTCCCTAAAAGGTAATGTCGCATAGTAAGATGTATGTCCCATATCAAACGCATTCCTAGAAAAACATGCACTGAATGAATAAGCAGTTACTTCGATTACTAATCTTCACAGCATAATTTTTCTCTTATCTTGTAAACCTCTTTCTATCTTTTCATAAAGTTTGTCACTTATATTTCTTCATTGGTACATCAATGCCAGCAACGATGTGCGCATCTGGGCGACGCCTGTAGTGATTGTTTTCAAGGTATCGAGCCAAACTATTCATCGTTACTGTTTCAAATATTCAGTTGTTTCAGTACAGAGTCGCCGTGGACCTAGTGAAACTTGGTGTCTTTACAGCGCAGAGATGAGGGCTTATATGTATAAAAGCTGTCCTTGATTCTGGTGTAGTTTGAGGTGTCCTTCCTATATCTGTTTTTATATTCTATATAATGGATAATTACTACCATCACCTGCATCAAATTCCAGTAAATTCACATATTGGAGAAAAAATGTGAGACCACGTTCAGTTTTCCAATGGCAGCCTCTTTATGCCAAGAATGTAGGGATAACAGGGTAATAATACTGGTGTATATTCAAGA,873,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000094.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000094.png,,,,,457e6d15,False
2145,BBF10K_000106,Sc-pRPL18B,S. cerevisiae promoter RPL18B,Open Yeast Collection,pOpen-Sc-pRPL18B,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000005245,,S. cerevisiae,yeast,S. cerevisiae RPL18B promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAAGAGGATGTCCAATATTTTTTTTAAGGAATAAGGATACTTCAAGACTAGATTCCCCCCTGCATTCCCATCAGAACCGTAAACCTTGGCGCTTTCCTTGGGAAGTATTCAAGAAGTGCCTTGTCCGGTTTCTGTGGCTCACAAACCAGCGCGCCCGATATGGCTTTCTTTTCACTTATGAATGTACCAGTACGGGACAATTAGAACGCTCCTGTAACAATCTCTTTGCAAATGTGGGGTTACATTCTAACCATGTCACACTGCTGACGAAATTCAAAGTAAAAAAAAATGGGACCACGTCTTGAGAACGATAGATTTTCTTTATTTTACATTGAACAGTCGTTGTCTCAGCGCGCTTTATGTTTTCATTCATACTTCATATTATAAAATAACAAAAGAAGAATTTCATATTCACGCCCAAGAAATCAGGCTGCTTTCCAAATGCAATTGACACTTCATTAGCCATCACACAAAACTCTTTCTTGCTGGAGCTTCTTTTAAAAAAGACCTCAGTACACCAAACACGTTACCCGACCTCGTTATTTTACGACAACTATGATAAAATTCTGAAGAAAAAATAAAAAAATTTTCATACTTCTTGCTTTTATTTAAACCATTGAATGATTTCTTTTGAACAAAACTACCTGTTTCACCAAAGGAAATAGAAAGAAAAAATCAATTAGAAGAAAACAAAAAACAAAAATGTGAGACCACTAGATTAGCAAGGCACCCCTCCACTGTGCCCGGAACCTGTAGGGATAACAGGGTAATGGGATTGACTGTACTCTACCA,2823,AACGATGATGCTCACTCTCGGGTCTCTGGAGAAGAGGATGTCCAATATTTTTTTTAAGGAATAAGGATACTTCAAGACTAGATTCCCCCCTGCATTCCCATCAGAACCGTAAACCTTGGCGCTTTCCTTGGGAAGTATTCAAGAAGTGCCTTGTCCGGTTTCTGTGGCTCACAAACCAGCGCGCCCGATATGGCTTTCTTTTCACTTATGAATGTACCAGTACGGGACAATTAGAACGCTCCTGTAACAATCTCTTTGCAAATGTGGGGTTACATTCTAACCATGTCACACTGCTGACGAAATTCAAAGTAAAAAAAAATGGGACCACGTCTTGAGAACGATAGATTTTCTTTATTTTACATTGAACAGTCGTTGTCTCAGCGCGCTTTATGTTTTCATTCATACTTCATATTATAAAATAACAAAAGAAGAATTTCATATTCACGCCCAAGAAATCAGGCTGCTTTCCAAATGCAATTGACACTTCATTAGCCATCACACAAAACTCTTTCTTGCTGGAGCTTCTTTTAAAAAAGACCTCAGTACACCAAACACGTTACCCGACCTCGTTATTTTACGACAACTATGATAAAATTCTGAAGAAAAAATAAAAAAATTTTCATACTTCTTGCTTTTATTTAAACCATTGAATGATTTCTTTTGAACAAAACTACCTGTTTCACCAAAGGAAATAGAAAGAAAAAATCAATTAGAAGAAAACAAAAAACAAAAATGTGAGACCACTAGATTAGCAAGGCACCCCTCCACTGTGCCCGGAACCTGTAGGGATAACAGGGTAATGGGATTGACTGTACTCTACCA,822,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000106.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000106.png,,,,,b1039796,False
2146,BBF10K_000107,Sc-pSAC6,S. cerevisiae promoter SAC6,Open Yeast Collection,pOpen-Sc-pSAC6,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002536,,S. cerevisiae,yeast,S. cerevisiae SAC6 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTTTGAGAATGACCTTCCACGAGCTAAATTGAAAGGGAAGAATTTATTAGTTGAACTCAAGAAAGAAGAGGATGACGTGGGAAATGGCATAGAATCCCTTACTAAATCGAACACTAAACTGAACTCCATGCTGGCGAACGAAGGTAAGATACACAAAGCTAGTTTCCAGAAAAGTGTAAAATTTAAACTACCTGATAATATAGTGACTGAAGAAACCGTGGAACTTAAAGAAATAAAGGACTTGCTACTACAAATGTTGAGATGACAGCGAGAGATTGAATCAAGATTATCCAATATCGAACTTCAACTCACGGAAATACCGAAACATAAGTAATCATATCCCTTCTCACATTTTTTACACAGGAAGTAAGCAAGTTATGTTATATTTCCGACACTATAATTAATTCTTAGCAGTTAAAGGTGCTTTGTCTATATTACATTTACATACAGCTTGAGTGATCCTGACCGGATATAGGGTCCTATTTTCTTACGTGAACGGCTTTTCTTCTTGTTCCCGATGGCCTTCATGTGAAAAAGCACTCCTCGGGAGGCGGAAAAATATCAAAAGTACGGGGCGAAGTTTATAATGAAGATTTATCGATATAAATTTTGGTTATTTCAGGAGAACAAGAAAGCTCTTTACACTAAAATTATCAGAGAAGAAGCTGATATATTAGCCCTAAGGAGTACACCAAAACACAAATGTGAGACCACTGGACCCAATAAAAGGCCTACGATAATTCAGGCTCCCGATAGGGATAACAGGGTAATAGAAGGCCGCGGTACTGGATC,2823,AACGATGATGCTCACTCTCGGGTCTCTGGAGTTTGAGAATGACCTTCCACGAGCTAAATTGAAAGGGAAGAATTTATTAGTTGAACTCAAGAAAGAAGAGGATGACGTGGGAAATGGCATAGAATCCCTTACTAAATCGAACACTAAACTGAACTCCATGCTGGCGAACGAAGGTAAGATACACAAAGCTAGTTTCCAGAAAAGTGTAAAATTTAAACTACCTGATAATATAGTGACTGAAGAAACCGTGGAACTTAAAGAAATAAAGGACTTGCTACTACAAATGTTGAGATGACAGCGAGAGATTGAATCAAGATTATCCAATATCGAACTTCAACTCACGGAAATACCGAAACATAAGTAATCATATCCCTTCTCACATTTTTTACACAGGAAGTAAGCAAGTTATGTTATATTTCCGACACTATAATTAATTCTTAGCAGTTAAAGGTGCTTTGTCTATATTACATTTACATACAGCTTGAGTGATCCTGACCGGATATAGGGTCCTATTTTCTTACGTGAACGGCTTTTCTTCTTGTTCCCGATGGCCTTCATGTGAAAAAGCACTCCTCGGGAGGCGGAAAAATATCAAAAGTACGGGGCGAAGTTTATAATGAAGATTTATCGATATAAATTTTGGTTATTTCAGGAGAACAAGAAAGCTCTTTACACTAAAATTATCAGAGAAGAAGCTGATATATTAGCCCTAAGGAGTACACCAAAACACAAATGTGAGACCACTGGACCCAATAAAAGGCCTACGATAATTCAGGCTCCCGATAGGGATAACAGGGTAATAGAAGGCCGCGGTACTGGATC,822,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000107.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000107.png,,,,,eb0c56ab,False
2147,BBF10K_000129,Sc-pSte5,S. cerevisiae promoter Ste5,Open Yeast Collection,pOpen-Sc-pSte5,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002510,,S. cerevisiae,yeast,S. cerevisiae Ste5 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGCACGAAGTGACTGACAGAATACTGACATCAGCTGATTTCTCATAGAGCTGTTTCTCTGATAACACGTTGTTTGAACATCGACAAGATGAAAAACTAGAAGTATCAAGTTTCCTTTAAAGGGATATATAACAGATTCTAAAACTGACAGAAATATTTCGAGTGAAGAAGAAGCGTTAAATATTGGATCTTTCCGCAGTTCTACTCTGATACATTTTTGAAGTAGGAGAGTCATTTAGAAGGCGTATTGCTCAATAGTAGAAAGCAGGCCTGTGCACATGAATTAATTAAAAAATATAAAGGTAGTGATTAGACGACACATGTCCATAGGTAACCTGTCATAATTTTGAACAATTTCCCTTCTTTTCTTTTTTATTTTTGGGTGCGGCGATATGTAGCTTGTTAATTTACACATCATGTACTTTTCTGCATCAAAATATGAAAGGCGATAGTAGCTAAAGAAAATACCGAGAATTTCCTCGAAAAGTTGACGACAAAAGAAAGGCATAAAAAAGTAATTTGAAAATATTTTAAAACTGTTTTAACCCATCTAGCATCCGCGCTAAAAAAGGAAGATACAGGATACAGCGGAAACAACTTTTAAAATGTGAGACCACTTCGATTGGCAAGGACTGTCACAAACGACTACGGTGTCGTAGGGATAACAGGGTAATTTTCACGAATGACGCATGGCG,2724,AACGATGATGCTCACTCTCGGGTCTCTGGAGCACGAAGTGACTGACAGAATACTGACATCAGCTGATTTCTCATAGAGCTGTTTCTCTGATAACACGTTGTTTGAACATCGACAAGATGAAAAACTAGAAGTATCAAGTTTCCTTTAAAGGGATATATAACAGATTCTAAAACTGACAGAAATATTTCGAGTGAAGAAGAAGCGTTAAATATTGGATCTTTCCGCAGTTCTACTCTGATACATTTTTGAAGTAGGAGAGTCATTTAGAAGGCGTATTGCTCAATAGTAGAAAGCAGGCCTGTGCACATGAATTAATTAAAAAATATAAAGGTAGTGATTAGACGACACATGTCCATAGGTAACCTGTCATAATTTTGAACAATTTCCCTTCTTTTCTTTTTTATTTTTGGGTGCGGCGATATGTAGCTTGTTAATTTACACATCATGTACTTTTCTGCATCAAAATATGAAAGGCGATAGTAGCTAAAGAAAATACCGAGAATTTCCTCGAAAAGTTGACGACAAAAGAAAGGCATAAAAAAGTAATTTGAAAATATTTTAAAACTGTTTTAACCCATCTAGCATCCGCGCTAAAAAAGGAAGATACAGGATACAGCGGAAACAACTTTTAAAATGTGAGACCACTTCGATTGGCAAGGACTGTCACAAACGACTACGGTGTCGTAGGGATAACAGGGTAATTTTCACGAATGACGCATGGCG,723,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000129.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000129.png,,,,,b06e4d39,False
2148,BBF10K_000134,Sc-pTDH3,S. cerevisiae promoter TDH3,Open Yeast Collection,pOpen-Sc-pTDH3,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003424,,S. cerevisiae,yeast,S. cerevisiae TDH3 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTCATTATCAATACTCGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAGAACTTAGTTTCGAAATGTGAGACCAGAACATAGCATTCACGGGGCCCTATGAGTGACGACGGCGTTAGGGATAACAGGGTAATTCCGCATCCCCGCCATGTTTA,2767,AACGATGATGCTCACTCTCGGGTCTCTGGAGTCATTATCAATACTCGCCATTTCAAAGAATACGTAAATAATTAATAGTAGTGATTTTCCTAACTTTATTTAGTCAAAAAATTAGCCTTTTAATTCTGCTGTAACCCGTACATGCCCAAAATAGGGGGCGGGTTACACAGAATATATAACATCGTAGGTGTCTGGGTGAACAGTTTATTCCTGGCATCCACTAAATATAATGGAGCCCGCTTTTTAAGCTGGCATCCAGAAAAAAAAAGAATCCCAGCACCAAAATATTGTTTTCTTCACCAACCATCAGTTCATAGGTCCATTCTCTTAGCGCAACTACAGAGAACAGGGGCACAAACAGGCAAAAAACGGGCACAACCTCAATGGAGTGATGCAACCTGCCTGGAGTAAATGATGACACAAGGCAATTGACCCACGCATGTATCTATCTCATTTTCTTACACCTTCTATTACCTTCTGCTCTCTCTGATTTGGAAAAAGCTGAAAAAAAAGGTTGAAACCAGTTCCCTGAAATTATTCCCCTACTTGACTAATAAGTATATAAAGACGGTAGGTATTGATTGTAATTCTGTAAATCTATTTCTTAAACTTCTTAAATTCTACTTTTATAGTTAGTCTTTTTTTTAGTTTTAAAACACCAGAACTTAGTTTCGAAATGTGAGACCAGAACATAGCATTCACGGGGCCCTATGAGTGACGACGGCGTTAGGGATAACAGGGTAATTCCGCATCCCCGCCATGTTTA,766,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000134.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000134.png,,,,,a2f3a97e,False
2149,BBF10K_000151,Sc-pTrp1,S. cerevisiae promoter Trp1,Open Yeast Collection,pOpen-Sc-pTrp1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002414,,S. cerevisiae,yeast,S. cerevisiae Trp1 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAACGACATTACTATATATATAATATAGGAAGCATTTAATAGAACAGCATCGTAATATATGTGTACTTTGCAGTTATGACGCCAGATGGCAGTAGTGGAAGATATTCTTTATTGAAAAATAGCTTGTCACCTTACGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTAATGTGAGACCGATATCAGACAACAATCTGAGGCTGGGAATTAAGTTTTGCAGTACTCTAGGGATAACAGGGTAATGCGTCTTGAAGTCCGTGAGGT,2411,AACGATGATGCTCACTCTCGGGTCTCTGGAGAACGACATTACTATATATATAATATAGGAAGCATTTAATAGAACAGCATCGTAATATATGTGTACTTTGCAGTTATGACGCCAGATGGCAGTAGTGGAAGATATTCTTTATTGAAAAATAGCTTGTCACCTTACGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTAATGTGAGACCGATATCAGACAACAATCTGAGGCTGGGAATTAAGTTTTGCAGTACTCTAGGGATAACAGGGTAATGCGTCTTGAAGTCCGTGAGGT,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000151.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000151.png,,,,,c19b9d34,False
2150,BBF10K_000152,Sc-pUra3,S. cerevisiae promoter Ura3,Open Yeast Collection,pOpen-Sc-pUra3,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000747,,S. cerevisiae,yeast,S. cerevisiae Ura3 promoter using standard Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,AATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTCAATTCATCATTTTATTTTTATTCTTTTTTTTGATTTCGGTTTCTTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCTGGAAACGAAGATAAATCAATGTGAGACCCTCGAGAGACAAGCCTTAACCGTAGGGGGGCAAATCCACTTTCCATGTAGGGATAACAGGGTAATACTTGCAACTGCCGGATTGCG,2344,AACGATGATGCTCACTCTCGGGTCTCTGGAGTCAATTCATCATTTTATTTTTATTCTTTTTTTTGATTTCGGTTTCTTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACCTGCTGGAAACGAAGATAAATCAATGTGAGACCCTCGAGAGACAAGCCTTAACCGTAGGGGGGCAAATCCACTTTCCATGTAGGGATAACAGGGTAATACTTGCAACTGCCGGATTGCG,343,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000152.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000152.png,,,,,b710eb41,False
2151,BBF10K_000163,AB_Sc-pCCW12,S. cerevisiae promoter CCW12 strong (AB enhanced),Open Yeast Collection,pOpen-AB_Sc-pCCW12,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000004100,,S. cerevisiae,yeast,S. cerevisiae CCW12 strong promoter using enhanced (AB) Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,TACT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGCACCCATGAACCACACGGTTAGTCCAAAAGGGGCAGTTCAGATTCCAGATGCGGGAATTAGCTTGCTGCCACCCTCACCTCACTAACGCTGCGGTGTGCGGATACTTCATGCTATTTATAGACGCGCGTGTCGGAATCAGCACGCGCAAGAACCAAATGGGAAAATCGGAATGGGTCCAGAACTGCTTTGAGTGCTGGCTATTGGCGTCTGATTTCCGTTTTGGGAATCCTTTGCCGCGCGCCCCTCTCAAAACTCCGCACAAGTCCCAGAAAGCGGGAAAGAAATAAAACGCCACCAAATAAAATAAAATAAAAGCCAATCCTCGAAGCGTGGGTGGTAGGCCCTGGATTATCCCGTACAAGTATTTCTCAGGAGTAAAAAAACCGTTTGTTTTGGAATTTCCCATTTCGCGGCCACCTACGCCGCTATCTTTGCAACAACTATCTGCGATAACTCAGCAAATTTTGCATATTCGTGTTGCAGTATTGCGATAATGGGAGTCTTACTTCCAACATAACGGCAGAAAGAAATGTGAGAAAATTTTGCATCCTTTGCCTCCGTTCAAGTATATAAAGTCGGCATGCTTGATAATCTTTCTTTCCATCCTACATTGTTCTAATTATTCTTATTCTCCTTTATTCTTTCCTAACATACCAAGAAATTAATCTTCTGTCATTCGCTTAAACACTATATCAATAATACTTGAGACCAGACACAAGGCTGATTCCAGTCCGTAGATCCTATCTCCGATTAGGGATAACAGGGTAATGGCAATTTCCTTAAACCCTGG,2823,AACGATGATGCTCACTCTCGGGTCTCTGGAGCACCCATGAACCACACGGTTAGTCCAAAAGGGGCAGTTCAGATTCCAGATGCGGGAATTAGCTTGCTGCCACCCTCACCTCACTAACGCTGCGGTGTGCGGATACTTCATGCTATTTATAGACGCGCGTGTCGGAATCAGCACGCGCAAGAACCAAATGGGAAAATCGGAATGGGTCCAGAACTGCTTTGAGTGCTGGCTATTGGCGTCTGATTTCCGTTTTGGGAATCCTTTGCCGCGCGCCCCTCTCAAAACTCCGCACAAGTCCCAGAAAGCGGGAAAGAAATAAAACGCCACCAAATAAAATAAAATAAAAGCCAATCCTCGAAGCGTGGGTGGTAGGCCCTGGATTATCCCGTACAAGTATTTCTCAGGAGTAAAAAAACCGTTTGTTTTGGAATTTCCCATTTCGCGGCCACCTACGCCGCTATCTTTGCAACAACTATCTGCGATAACTCAGCAAATTTTGCATATTCGTGTTGCAGTATTGCGATAATGGGAGTCTTACTTCCAACATAACGGCAGAAAGAAATGTGAGAAAATTTTGCATCCTTTGCCTCCGTTCAAGTATATAAAGTCGGCATGCTTGATAATCTTTCTTTCCATCCTACATTGTTCTAATTATTCTTATTCTCCTTTATTCTTTCCTAACATACCAAGAAATTAATCTTCTGTCATTCGCTTAAACACTATATCAATAATACTTGAGACCAGACACAAGGCTGATTCCAGTCCGTAGATCCTATCTCCGATTAGGGATAACAGGGTAATGGCAATTTCCTTAAACCCTGG,822,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000163.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000163.png,,,,,7fd2f5d3,False
2152,BBF10K_000167,AB_Sc-pCYC1,S. cerevisiae promoter CYC1 (AB enhanced),Open Yeast Collection,pOpen-AB_Sc-pCYC1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003809,,S. cerevisiae,yeast,S. cerevisiae CYC1 promoter using enhanced (AB) Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,TACT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGTGCAGATCCGCCAGGCGTGTATATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACATTAGGACCTTTGCAGCATAAATTACTATACTTCTATAGACACACAAACACAAATACACACACTAAATTAATATACTTGAGACCAGACATGGGATTGACCACACCTACCTTTGCCTTAGTTTTGCTAGGGATAACAGGGTAATAGGGATCGGACATTAACCCTC,2411,AACGATGATGCTCACTCTCGGGTCTCTGGAGAGCGTTGGTTGGTGGATCAAGCCCACGCGTAGGCAATCCTCGTGCAGATCCGCCAGGCGTGTATATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACATTAGGACCTTTGCAGCATAAATTACTATACTTCTATAGACACACAAACACAAATACACACACTAAATTAATATACTTGAGACCAGACATGGGATTGACCACACCTACCTTTGCCTTAGTTTTGCTAGGGATAACAGGGTAATAGGGATCGGACATTAACCCTC,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000167.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/7737504/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000167.png,,,,,345af437,False
2154,BBF10K_000180,AB_Pp-pGAP,Pichia GAP Promoter (enhanced),Open Yeast Collection,pOpen-AB_Pp-pGAP,pBuild,M13 For,M13 Rev,,,,,,,P. pastoris,P. pastoris,Pichia GAP Promoter using enhanced (AB) Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,TACT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTATTACTTGAGACCAGAGTTGCACCTAGAATCCGCAGGGTAAACTCGAATAGGGGTAGGGATAACAGGGTAATGGGCGCGCGCACTGTAAGATT,2600,AACGATGATGCTCACTCTCGGGTCTCTGGAGTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTATTACTTGAGACCAGAGTTGCACCTAGAATCCGCAGGGTAAACTCGAATAGGGGTAGGGATAACAGGGTAATGGGCGCGCGCACTGTAAGATT,599,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000180.gb,Scott Pownall & Isaac Larkin,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000180.png,,,,,055f0f69,False
2155,BBF10K_000181,AB_Pp-pAOX1,Pichia AOX1 Promoter (enhanced),Open Yeast Collection,pOpen-AB_Pp-pAOX1,pBuild,M13 For,M13 Rev,,,,,,,P. pastoris,P. pastoris,Pichia AOX1 Promoter using enhanced (AB) Freegenes syntax,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,promoter,GGAG,TACT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGGAGGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGTACTTGAGACCAGATAGATGCTCCGTCAAGCCCAGGCTGAGATTTGTCAGATTAGGGATAACAGGGTAATTAGACGAAAGCCCGTTGTAGT,3062,AACGATGATGCTCACTCTCGGGTCTCTGGAGGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGTACTTGAGACCAGATAGATGCTCCGTCAAGCCCAGGCTGAGATTTGTCAGATTAGGGATAACAGGGTAATTAGACGAAAGCCCGTTGTAGT,1061,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000181.gb,Scott Pownall & Isaac Larkin,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000181.png,,,,,4e9ce1ec,False
2156,BBF10K_000195,ScLYS2,S. cerevisiae cds LYS2,Open Yeast Collection,pOpen-ScLYS2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000319,,S. cerevisiae,S. cerevisiae,Complements lysine auxotrophy,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGACTAACGAAAAGGTCTGGATAGAGAAGTTGGATAATCCAACTCTTTCAGTGTTACCACATGACTTTTTACGCCCACAACAAGAACCTTATACGAAACAAGCTACATATTCGTTACAGCTACCTCAGCTCGATGTGCCTCATGATAGTTTTTCTAACAAATACGCTGTCGCTTTGAGTGTATGGGCTGCATTGATATATAGAGTAACCGGTGACGATGATATTGTTCTTTATATTGCGAATAACAAAATCTTAAGATTCAATATTCAACCAACGTGGTCATTTAATGAGCTGTATTCTACAATTAACAATGAGTTGAACAAGCTCAATTCTATTGAGGCCAATTTTTCCTTTGACGAACTAGCTGAAAAAATTCAAAGTTGCCAAGACCTGGAAAGGACCCCTCAGTTGTTCCGTTTGGCCTTTTTGGAAAACCAAGATTTCAAATTAGACGAGTTCAAGCATCATTTAGTGGACTTTGCTTTGAATTTGGATACCAGTAATAATGCGCATGTTTTGAACTTAATTTATAACAGCTTACTGTATTCGAATGAAAGAGTAACCATTGTTGCGGACCAATTTACTCAATATTTGACTGCTGCGCTAAGCGATCCATCCAATTGCATAACTAAAATCTCTCTGATCACCGCATCATCCAAGGATAGTTTACCTGATCCAACTAAGAACTTGGGCTGGTGCGATTTCGTGGGGTGTATTCACGACATTTTCCAGGACAATGCTGAAGCCTTCCCAGAGAGAACCTGTGTTGTGGAGACTCCAACACTAAATTCCGACAAGTCCCGTTCTTTCACTTATCGTGACATCAACCGCACTTCTAACATAGTTGCCCATTATTTGATTAAAACAGGTATCAAAAGAGGTGATGTAGTGATGATCTATTCTTCTAGGGGTGTGGATTTGATGGTATGTGTGATGGGTGTCTTGAAAGCCGGCGCAACCTTTTCAGTTATCGACCCTGCATATCCCCCAGCCAGACAAACCATTTACTTAGGTGTTGCTAAACCACGTGGGTTGATTGTTATTAGAGCTGCTGGACAATTGGATCAACTGGTAGAAGATTACATCAATGATGAATTGGAGATTGTTTCAAGAATCAATTCCATTGCTATTCAAGAAAATGGTACCATTGAAGGTGGCAAATTGGACAATGGCGAGGATGTTTTGGCTCCATATGATCACTACAAAGACACCAGAACAGGTGTTGTAGTTGGACCAGATTCCAACCCAACCCTATCTTTCACATCTGGTTCCGAAGGTATTCCTAAGGGTGTTCTTGGTAGACATTTTTCCTTGGCTTATTATTTCAATTGGATGTCCAAAAGGTTCAACTTAACAGAAAATGATAAATTCACAATGCTGAGCGGTATTGCACATGATCCAATTCAAAGAGATATGTTTACACCATTATTTTTAGGTGCCCAATTGTATGTCCCTACTCAAGATGATATTGGTACACCGGGCCGTTTAGCGGAATGGATGAGTAAGTATGGTTGCACAGTTACCCATTTAACACCAGCCATGGGTCAATTACTTACTGCCCAAGCTACTACACCATTCCCTAAGTTACATCATGCGTTCTTTGTGGGTGACATTTTAACAAAACGTGATTGTCTGAGGTTACAAACCTTGGCAGAAAATTGCCGTATTGTTAATATGTACGGTACCACTGAAACACAGCGTGCAGTTTCTTATTTCGAAGTTAAATCAAAAAATGACGATCCAAACTTTTTGAAAAAATTGAAAGATGTCATGCCTGCTGGTAAAGGTATGTTGAACGTTCAGCTGCTAGTTGTTAACAGGAACGATCGTACTCAAATATGTGGTATTGGCGAAATAGGTGAGATTTATGTTCGTGCAGGCGGTTTGGCCGAAGGTTATAGAGGATTACCAGAATTGAATAAAGAAAAATTTGTGAACAACTGGTTTGTTGAAAAAGATCACTGGAATTATTTGGATAAGGATAATGGTGAACCTTGGAGACAATTCTGGTTAGGTCCAAGAGATAGATTGTACAGAACGGGTGATTTAGGTCGTTATCTACCAAACGGTGACTGTGAATGTTGCGGTAGGGCTGATGATCAAGTTAAAATTCGTGGGTTCAGAATCGAATTAGGAGAAATAGATACGCACATTTCCCAACATCCATTGGTAAGAGAAAACATTACTTTAGTTCGCAAAAATGCCGACAATGAGCCAACATTGATCACATTTATGGTCCCAAGATTTGACAAGCCAGATGACTTGTCTAAGTTCCAAAGTGATGTTCCAAAGGAGGTTGAAACTGACCCTATAGTTAAGGGCTTAATCGGTTACCATCTTTTATCCAAGGACATCAGGACTTTCTTAAAGAAAAGATTGGCTAGTTATGCTATGCCTTCCTTGATTGTGGTTATGGATAAACTACCATTGAATCCAAATGGTAAAGTTGATAAGCCTAAACTTCAATTCCCAACTCCCAAGCAATTAAATTTGGTAGCTGAAAATACAGTTTCTGAAACTGACGACTCTCAGTTTACCAATGTTGAGCGCGAGGTTAGAGACTTATGGTTAAGTATATTACCTACCAAGCCAGCATCTGTATCACCAGATGATTCGTTTTTCGATTTAGGTGGTCATTCTATCTTGGCTACCAAAATGATTTTTACCTTAAAGAAAAAGCTGCAAGTTGATTTACCATTGGGCACAATTTTCAAGTATCCAACGATAAAGGCCTTTGCCGCGGAAATTGACAGAATTAAATCATCGGGTGGATCATCTCAAGGTGAGGTCGTCGAAAATGTCACTGCAAATTATGCGGAGGACGCCAAGAAATTGGTTGAAACGCTACCAAGTTCGTACCCCAGTAGGGAATATTTTGTTGAACCTAATAGTGCCGAAGGAAAAACAACAATTAATGTGTTTGTTACCGGTGTCACAGGATTTCTGGGCTCCTACATCCTTGCAGATTTGTTAGGACGTTCTCCAAAGAACTACAGTTTCAAAGTGTTTGCCCACGTCAGGGCCAAGGATGAAGAAGCTGCATTTGCAAGATTACAAAAGGCAGGTATCACCTATGGTACTTGGAACGAAAAATTTGCCTCAAATATTAAAGTTGTATTAGGCGATTTATCTAAAAGCCAATTTGGTCTTTCAGATGAGAAGTGGATGGATTTGGCAAACACAGTTGATATAATTATCCATAATGGTGCGTTAGTTCACTGGGTTTATCCATATGCCAAATTGAGGGACCCAAATGTTATTTCAACTATCAATGTTATGAGCTTAGCCGCCGTCGGCAAGCCAAAGTTCTTTGACTTTGTTTCCTCCACTTCTACTCTTGACACTGAATACTACTTTAATTTGTCAGATAAACTTGTTAGCGAAGGGAAGCCAGGCATTTTAGAATCAGACGATTTAATGAACTCTGCAAGCGGGCTCACTGGTGGATATGGTCAGTCCAAATGGGCTGCTGAGTACATCATTAGACGTGCCGGTGAAAGGGGCCTACGTGGGTGTATTGTCAGACCAGGTTACGTAACAGGTGCCTCTGCCAATGGTTCTTCAAACACAGATGATTTCTTATTGAGATTTTTGAAAGGTTCAGTCCAATTAGGTAAGATTCCAGATATCGAAAATTCCGTGAATATGGTTCCAGTAGATCATGTTGCTCGTGTTGTTGTTGCTACGTCTTTGAATCCTCCCAAAGAAAATGAATTGGCCGTTGCTCAAGTAACGGGTCACCCAAGAATATTATTCAAAGACTACTTGTATACTTTACACGATTATGGTTACGATGTCGAAATCGAAAGCTATTCTAAATGGAAGAAATCATTGGAGGCGTCTGTTATTGACAGGAATGAAGAAAATGCGTTGTATCCTTTGCTACACATGGTCTTAGACAACTTACCTGAAAGTACCAAAGCTCCGGAACTAGACGATAGGAACGCCGTGGCATCTTTAAAGAAAGACACCGCATGGACAGGTGTTGATTGGTCTAATGGAATAGGTGTTACTCCAGAAGAGGTTGGTATATATATTGCATTTTTAAACAAGGTTGGATTTTTACCTCCACCAACTCATAATGACAAACTTCCACTGCCAAGTATAGAACTAACTCAAGCGCAAATAAGTCTAGTTGCTTCAGGTGCTGGTGCTCGTGGAAGCTCCGCAGCAGCTTGAAGAGCAAGCTTTGAGACCAGATAGTCACGCACAAGACCATCTCTAAGTGATAAAAACTTTAGGGATAACAGGGTAATGTGAATGGCCAATGTGAAAGA,6306,AACGATGATGCTCACTCTCGGGTCTCTAATGACTAACGAAAAGGTCTGGATAGAGAAGTTGGATAATCCAACTCTTTCAGTGTTACCACATGACTTTTTACGCCCACAACAAGAACCTTATACGAAACAAGCTACATATTCGTTACAGCTACCTCAGCTCGATGTGCCTCATGATAGTTTTTCTAACAAATACGCTGTCGCTTTGAGTGTATGGGCTGCATTGATATATAGAGTAACCGGTGACGATGATATTGTTCTTTATATTGCGAATAACAAAATCTTAAGATTCAATATTCAACCAACGTGGTCATTTAATGAGCTGTATTCTACAATTAACAATGAGTTGAACAAGCTCAATTCTATTGAGGCCAATTTTTCCTTTGACGAACTAGCTGAAAAAATTCAAAGTTGCCAAGACCTGGAAAGGACCCCTCAGTTGTTCCGTTTGGCCTTTTTGGAAAACCAAGATTTCAAATTAGACGAGTTCAAGCATCATTTAGTGGACTTTGCTTTGAATTTGGATACCAGTAATAATGCGCATGTTTTGAACTTAATTTATAACAGCTTACTGTATTCGAATGAAAGAGTAACCATTGTTGCGGACCAATTTACTCAATATTTGACTGCTGCGCTAAGCGATCCATCCAATTGCATAACTAAAATCTCTCTGATCACCGCATCATCCAAGGATAGTTTACCTGATCCAACTAAGAACTTGGGCTGGTGCGATTTCGTGGGGTGTATTCACGACATTTTCCAGGACAATGCTGAAGCCTTCCCAGAGAGAACCTGTGTTGTGGAGACTCCAACACTAAATTCCGACAAGTCCCGTTCTTTCACTTATCGTGACATCAACCGCACTTCTAACATAGTTGCCCATTATTTGATTAAAACAGGTATCAAAAGAGGTGATGTAGTGATGATCTATTCTTCTAGGGGTGTGGATTTGATGGTATGTGTGATGGGTGTCTTGAAAGCCGGCGCAACCTTTTCAGTTATCGACCCTGCATATCCCCCAGCCAGACAAACCATTTACTTAGGTGTTGCTAAACCACGTGGGTTGATTGTTATTAGAGCTGCTGGACAATTGGATCAACTGGTAGAAGATTACATCAATGATGAATTGGAGATTGTTTCAAGAATCAATTCCATTGCTATTCAAGAAAATGGTACCATTGAAGGTGGCAAATTGGACAATGGCGAGGATGTTTTGGCTCCATATGATCACTACAAAGACACCAGAACAGGTGTTGTAGTTGGACCAGATTCCAACCCAACCCTATCTTTCACATCTGGTTCCGAAGGTATTCCTAAGGGTGTTCTTGGTAGACATTTTTCCTTGGCTTATTATTTCAATTGGATGTCCAAAAGGTTCAACTTAACAGAAAATGATAAATTCACAATGCTGAGCGGTATTGCACATGATCCAATTCAAAGAGATATGTTTACACCATTATTTTTAGGTGCCCAATTGTATGTCCCTACTCAAGATGATATTGGTACACCGGGCCGTTTAGCGGAATGGATGAGTAAGTATGGTTGCACAGTTACCCATTTAACACCAGCCATGGGTCAATTACTTACTGCCCAAGCTACTACACCATTCCCTAAGTTACATCATGCGTTCTTTGTGGGTGACATTTTAACAAAACGTGATTGTCTGAGGTTACAAACCTTGGCAGAAAATTGCCGTATTGTTAATATGTACGGTACCACTGAAACACAGCGTGCAGTTTCTTATTTCGAAGTTAAATCAAAAAATGACGATCCAAACTTTTTGAAAAAATTGAAAGATGTCATGCCTGCTGGTAAAGGTATGTTGAACGTTCAGCTGCTAGTTGTTAACAGGAACGATCGTACTCAAATATGTGGTATTGGCGAAATAGGTGAGATTTATGTTCGTGCAGGCGGTTTGGCCGAAGGTTATAGAGGATTACCAGAATTGAATAAAGAAAAATTTGTGAACAACTGGTTTGTTGAAAAAGATCACTGGAATTATTTGGATAAGGATAATGGTGAACCTTGGAGACAATTCTGGTTAGGTCCAAGAGATAGATTGTACAGAACGGGTGATTTAGGTCGTTATCTACCAAACGGTGACTGTGAATGTTGCGGTAGGGCTGATGATCAAGTTAAAATTCGTGGGTTCAGAATCGAATTAGGAGAAATAGATACGCACATTTCCCAACATCCATTGGTAAGAGAAAACATTACTTTAGTTCGCAAAAATGCCGACAATGAGCCAACATTGATCACATTTATGGTCCCAAGATTTGACAAGCCAGATGACTTGTCTAAGTTCCAAAGTGATGTTCCAAAGGAGGTTGAAACTGACCCTATAGTTAAGGGCTTAATCGGTTACCATCTTTTATCCAAGGACATCAGGACTTTCTTAAAGAAAAGATTGGCTAGTTATGCTATGCCTTCCTTGATTGTGGTTATGGATAAACTACCATTGAATCCAAATGGTAAAGTTGATAAGCCTAAACTTCAATTCCCAACTCCCAAGCAATTAAATTTGGTAGCTGAAAATACAGTTTCTGAAACTGACGACTCTCAGTTTACCAATGTTGAGCGCGAGGTTAGAGACTTATGGTTAAGTATATTACCTACCAAGCCAGCATCTGTATCACCAGATGATTCGTTTTTCGATTTAGGTGGTCATTCTATCTTGGCTACCAAAATGATTTTTACCTTAAAGAAAAAGCTGCAAGTTGATTTACCATTGGGCACAATTTTCAAGTATCCAACGATAAAGGCCTTTGCCGCGGAAATTGACAGAATTAAATCATCGGGTGGATCATCTCAAGGTGAGGTCGTCGAAAATGTCACTGCAAATTATGCGGAGGACGCCAAGAAATTGGTTGAAACGCTACCAAGTTCGTACCCCAGTAGGGAATATTTTGTTGAACCTAATAGTGCCGAAGGAAAAACAACAATTAATGTGTTTGTTACCGGTGTCACAGGATTTCTGGGCTCCTACATCCTTGCAGATTTGTTAGGACGTTCTCCAAAGAACTACAGTTTCAAAGTGTTTGCCCACGTCAGGGCCAAGGATGAAGAAGCTGCATTTGCAAGATTACAAAAGGCAGGTATCACCTATGGTACTTGGAACGAAAAATTTGCCTCAAATATTAAAGTTGTATTAGGCGATTTATCTAAAAGCCAATTTGGTCTTTCAGATGAGAAGTGGATGGATTTGGCAAACACAGTTGATATAATTATCCATAATGGTGCGTTAGTTCACTGGGTTTATCCATATGCCAAATTGAGGGACCCAAATGTTATTTCAACTATCAATGTTATGAGCTTAGCCGCCGTCGGCAAGCCAAAGTTCTTTGACTTTGTTTCCTCCACTTCTACTCTTGACACTGAATACTACTTTAATTTGTCAGATAAACTTGTTAGCGAAGGGAAGCCAGGCATTTTAGAATCAGACGATTTAATGAACTCTGCAAGCGGGCTCACTGGTGGATATGGTCAGTCCAAATGGGCTGCTGAGTACATCATTAGACGTGCCGGTGAAAGGGGCCTACGTGGGTGTATTGTCAGACCAGGTTACGTAACAGGTGCCTCTGCCAATGGTTCTTCAAACACAGATGATTTCTTATTGAGATTTTTGAAAGGTTCAGTCCAATTAGGTAAGATTCCAGATATCGAAAATTCCGTGAATATGGTTCCAGTAGATCATGTTGCTCGTGTTGTTGTTGCTACGTCTTTGAATCCTCCCAAAGAAAATGAATTGGCCGTTGCTCAAGTAACGGGTCACCCAAGAATATTATTCAAAGACTACTTGTATACTTTACACGATTATGGTTACGATGTCGAAATCGAAAGCTATTCTAAATGGAAGAAATCATTGGAGGCGTCTGTTATTGACAGGAATGAAGAAAATGCGTTGTATCCTTTGCTACACATGGTCTTAGACAACTTACCTGAAAGTACCAAAGCTCCGGAACTAGACGATAGGAACGCCGTGGCATCTTTAAAGAAAGACACCGCATGGACAGGTGTTGATTGGTCTAATGGAATAGGTGTTACTCCAGAAGAGGTTGGTATATATATTGCATTTTTAAACAAGGTTGGATTTTTACCTCCACCAACTCATAATGACAAACTTCCACTGCCAAGTATAGAACTAACTCAAGCGCAAATAAGTCTAGTTGCTTCAGGTGCTGGTGCTCGTGGAAGCTCCGCAGCAGCTTGAAGAGCAAGCTTTGAGACCAGATAGTCACGCACAAGACCATCTCTAAGTGATAAAAACTTTAGGGATAACAGGGTAATGTGAATGGCCAATGTGAAAGA,4305,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000195.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/6350829,,we are unaware,freegenes.github.io/genes/images/BBF10K_000195.png,,,,,91953fbe,False
2157,BBF10K_000196,ScMET17,S. cerevisiae cds MET17,Open Yeast Collection,pOpen-ScMET17,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000004294,,S. cerevisiae,S. cerevisiae,Complements methionine auxotrophy,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGCCATCTCATTTCGATACTGTTCAACTACACGCCGGCCAAGAGAACCCTGGTGACAATGCTCACAGATCCAGAGCTGTACCAATTTACGCCACCACTTCTTATGTTTTCGAAAACTCTAAGCATGGTTCGCAATTGTTTGGCCTAGAAGTTCCAGGTTACGTCTATTCCCGTTTCCAAAACCCAACCAGTAATGTTTTGGAAGAAAGAATTGCTGCTTTAGAAGGTGGTGCTGCTGCTTTGGCTGTTTCCTCCGGTCAAGCCGCTCAAACCCTTGCCATCCAAGGTTTGGCACACACTGGTGACAACATCGTTTCCACTTCTTACTTATACGGTGGTACTTATAACCAGTTCAAAATCTCGTTCAAAAGATTTGGTATCGAGGCTAGATTTGTTGAAGGTGACAATCCAGAAGAATTTGAAAAGGTCTTTGATGAAAGAACCAAGGCTGTTTATTTGGAAACCATTGGTAATCCAAAGTACAATGTTCCGGATTTTGAAAAAATTGTTGCAATTGCTCACAAACACGGTATTCCAGTTGTCGTTGACAACACATTTGGTGCCGGTGGTTACTTCTGTCAGCCAATTAAATACGGTGCTGATATTGTAACACATTCTGCTACCAAATGGATTGGTGGTCATGGTACTACTATCGGTGGTATTATTGTTGACTCTGGTAAGTTCCCATGGAAGGACTACCCAGAAAAGTTCCCTCAATTCTCTCAACCTGCCGAAGGATATCACGGTACTATCTACAATGAAGCCTACGGTAACTTGGCATACATCGTTCATGTTAGAACTGAACTATTAAGAGATTTGGGTCCATTGATGAACCCATTTGCCTCTTTCTTGCTACTACAAGGTGTTGAAACATTATCTTTGAGAGCTGAAAGACACGGTGAAAATGCATTGAAGTTAGCCAAATGGTTAGAACAATCCCCATACGTATCTTGGGTTTCATACCCTGGTTTAGCATCTCATTCTCATCATGAAAATGCTAAGAAGTATCTATCTAACGGTTTCGGTGGTGTCTTATCTTTCGGTGTAAAAGACTTACCAAATGCCGACAAGGAAACTGACCCATTCAAACTTTCTGGTGCTCAAGTTGTTGACAATTTAAAACTTGCCTCTAACTTGGCCAATGTTGGTGATGCCAAGACCTTAGTCATTGCTCCATACTTCACTACCCACAAACAATTAAATGACAAAGAAAAGTTGGCATCTGGTGTTACCAAGGACTTAATTCGTGTCTCTGTTGGTATCGAATTTATTGATGACATTATTGCAGACTTCCAGCAATCTTTTGAAACTGTTTTCGCTGGCCAAAAACCATGAAGAGCAAGCTTTGAGACCAGATTAGCCGACTTTCCTGGGCACTGCGAGAAATAGCATCTTAGGGATAACAGGGTAATTGTGATAGTCGAATAGTGTGA,3462,AACGATGATGCTCACTCTCGGGTCTCTAATGCCATCTCATTTCGATACTGTTCAACTACACGCCGGCCAAGAGAACCCTGGTGACAATGCTCACAGATCCAGAGCTGTACCAATTTACGCCACCACTTCTTATGTTTTCGAAAACTCTAAGCATGGTTCGCAATTGTTTGGCCTAGAAGTTCCAGGTTACGTCTATTCCCGTTTCCAAAACCCAACCAGTAATGTTTTGGAAGAAAGAATTGCTGCTTTAGAAGGTGGTGCTGCTGCTTTGGCTGTTTCCTCCGGTCAAGCCGCTCAAACCCTTGCCATCCAAGGTTTGGCACACACTGGTGACAACATCGTTTCCACTTCTTACTTATACGGTGGTACTTATAACCAGTTCAAAATCTCGTTCAAAAGATTTGGTATCGAGGCTAGATTTGTTGAAGGTGACAATCCAGAAGAATTTGAAAAGGTCTTTGATGAAAGAACCAAGGCTGTTTATTTGGAAACCATTGGTAATCCAAAGTACAATGTTCCGGATTTTGAAAAAATTGTTGCAATTGCTCACAAACACGGTATTCCAGTTGTCGTTGACAACACATTTGGTGCCGGTGGTTACTTCTGTCAGCCAATTAAATACGGTGCTGATATTGTAACACATTCTGCTACCAAATGGATTGGTGGTCATGGTACTACTATCGGTGGTATTATTGTTGACTCTGGTAAGTTCCCATGGAAGGACTACCCAGAAAAGTTCCCTCAATTCTCTCAACCTGCCGAAGGATATCACGGTACTATCTACAATGAAGCCTACGGTAACTTGGCATACATCGTTCATGTTAGAACTGAACTATTAAGAGATTTGGGTCCATTGATGAACCCATTTGCCTCTTTCTTGCTACTACAAGGTGTTGAAACATTATCTTTGAGAGCTGAAAGACACGGTGAAAATGCATTGAAGTTAGCCAAATGGTTAGAACAATCCCCATACGTATCTTGGGTTTCATACCCTGGTTTAGCATCTCATTCTCATCATGAAAATGCTAAGAAGTATCTATCTAACGGTTTCGGTGGTGTCTTATCTTTCGGTGTAAAAGACTTACCAAATGCCGACAAGGAAACTGACCCATTCAAACTTTCTGGTGCTCAAGTTGTTGACAATTTAAAACTTGCCTCTAACTTGGCCAATGTTGGTGATGCCAAGACCTTAGTCATTGCTCCATACTTCACTACCCACAAACAATTAAATGACAAAGAAAAGTTGGCATCTGGTGTTACCAAGGACTTAATTCGTGTCTCTGTTGGTATCGAATTTATTGATGACATTATTGCAGACTTCCAGCAATCTTTTGAAACTGTTTTCGCTGGCCAAAAACCATGAAGAGCAAGCTTTGAGACCAGATTAGCCGACTTTCCTGGGCACTGCGAGAAATAGCATCTTAGGGATAACAGGGTAATTGTGATAGTCGAATAGTGTGA,1461,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000196.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/3325778,,we are unaware,freegenes.github.io/genes/images/BBF10K_000196.png,,,,,5e0b8ce5,False
2158,BBF10K_000199,ScAUR1,S. cerevisiae cds AUR1,Open Yeast Collection,pOpen-ScAUR1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000001487,,S. cerevisiae,S. cerevisiae,Confers aureobasidin resistance,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGCAAACCCTTTTTCGAGATGGTTTCTATCAGAGAAACCTCCAAACTGCCATGTAGCCGATTTAGAAACAAGTTTAGATCCCCATCAAACGTTGTTGAAGGTGCAAAAATACAAACCCGCTTTAAGCGACTGGGTGCATTACATCTTCTTGGGATCAATCATGCTGTTTGTGTTCATTACTAATCCCGCACCTTGGATCTTCAAGATCCTTTTTTATTGTTTCTTGGGCACTTTATTCATCATTCCAGCTACGTCACAGTTTTTCTTCAATGCCTTGCCCATCCTAACATGGGTGGCGCTGTATTTCACTTCATCGTACTTTCCAGATGACCGCAGGCCTCCTATTACTGTCAAAGTGTTACCAGCGGTGGAAACAATTTTATACGGCGACAATTTAAGTGATATTCTTGCAACATCGACGAACTCCTTTTTGGACATTTTAGCATGGTTACCGTACGGACTATTTCATTTTGGGGCCCCATTTGTCGTTGCTGCCATCTTATTCGTATTTGGTCCACCAACTGTTTTGCAAGGTTATGCTTTTGCATTTGGTTATATGAACCTGTTTGGTGTTATCATGCAAAATGTCTTTCCAGCCGCTCCCCCATGGTATAAAATTCTCTATGGATTGCAATCAGCCAACTATGATATGCATGGCTCGCCTGGTGGATTAGCTAGAATTGATAAGCTACTCGGTATTAATATGTATACTACAGCTTTTTCAAATTCCTCCGTCATTTTCGGTGCTTTTCCTTCACTGCATTCCGGGTGTGCTACTATGGAAGCCCTGTTTTTCTGTTATTGTTTTCCAAAATTGAAGCCCTTGTTTATTGCTTATGTTTGCTGGTTATGGTGGTCAACTATGTATCTGACACACCATTATTTTGTAGACCTTATGGCAGGTTCTGTGCTGTCATACGTTATTTTCCAGTACACAAAGTACACACATTTACCAATTGTAGATACATCTCTTTTTTGCAGATGGTCATACACTTCAATTGAGAAATACGATATATCAAAGAGTGATCCATTGGCAGCTGATTCAAACGATATCGAAAGTGTCCCTTTGTCCAACTTGGAACTTGACTTTGATCTTAATATGACTGATGAACCCAGTGTAAGCCCTTCGTTATTTGATGGATCTACTTCTGTTTCTCGTTCGTCCGCCACGTCTATAACGTCACTAGGTGTAAAGAGGGCTTGAAGAGCAAGCTTTGAGACCAGATTAGCTGCCGATACTGGTTCTAAGCCTATTAGGTCGCATAGGGATAACAGGGTAATGACTGCGCACCCCTGATTACC,3333,AACGATGATGCTCACTCTCGGGTCTCTAATGGCAAACCCTTTTTCGAGATGGTTTCTATCAGAGAAACCTCCAAACTGCCATGTAGCCGATTTAGAAACAAGTTTAGATCCCCATCAAACGTTGTTGAAGGTGCAAAAATACAAACCCGCTTTAAGCGACTGGGTGCATTACATCTTCTTGGGATCAATCATGCTGTTTGTGTTCATTACTAATCCCGCACCTTGGATCTTCAAGATCCTTTTTTATTGTTTCTTGGGCACTTTATTCATCATTCCAGCTACGTCACAGTTTTTCTTCAATGCCTTGCCCATCCTAACATGGGTGGCGCTGTATTTCACTTCATCGTACTTTCCAGATGACCGCAGGCCTCCTATTACTGTCAAAGTGTTACCAGCGGTGGAAACAATTTTATACGGCGACAATTTAAGTGATATTCTTGCAACATCGACGAACTCCTTTTTGGACATTTTAGCATGGTTACCGTACGGACTATTTCATTTTGGGGCCCCATTTGTCGTTGCTGCCATCTTATTCGTATTTGGTCCACCAACTGTTTTGCAAGGTTATGCTTTTGCATTTGGTTATATGAACCTGTTTGGTGTTATCATGCAAAATGTCTTTCCAGCCGCTCCCCCATGGTATAAAATTCTCTATGGATTGCAATCAGCCAACTATGATATGCATGGCTCGCCTGGTGGATTAGCTAGAATTGATAAGCTACTCGGTATTAATATGTATACTACAGCTTTTTCAAATTCCTCCGTCATTTTCGGTGCTTTTCCTTCACTGCATTCCGGGTGTGCTACTATGGAAGCCCTGTTTTTCTGTTATTGTTTTCCAAAATTGAAGCCCTTGTTTATTGCTTATGTTTGCTGGTTATGGTGGTCAACTATGTATCTGACACACCATTATTTTGTAGACCTTATGGCAGGTTCTGTGCTGTCATACGTTATTTTCCAGTACACAAAGTACACACATTTACCAATTGTAGATACATCTCTTTTTTGCAGATGGTCATACACTTCAATTGAGAAATACGATATATCAAAGAGTGATCCATTGGCAGCTGATTCAAACGATATCGAAAGTGTCCCTTTGTCCAACTTGGAACTTGACTTTGATCTTAATATGACTGATGAACCCAGTGTAAGCCCTTCGTTATTTGATGGATCTACTTCTGTTTCTCGTTCGTCCGCCACGTCTATAACGTCACTAGGTGTAAAGAGGGCTTGAAGAGCAAGCTTTGAGACCAGATTAGCTGCCGATACTGGTTCTAAGCCTATTAGGTCGCATAGGGATAACAGGGTAATGACTGCGCACCCCTGATTACC,1332,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000199.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/8593016,,we are unaware,freegenes.github.io/genes/images/BBF10K_000199.png,,,,,4dc3fc1d,False
2159,BBF10K_000207,Cre,S. cerevisiae cds Cre recombinase,Open Yeast Collection,pOpen-Cre,pBuild,M13 For,M13 Rev,,,,,,https://www.uniprot.org/uniprot/P06956,P1 bacteriophage,yeast,"For Cre-LoxP recombination, Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage.","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGAGCAACTTGCTTACTGTTCATCAAAACCTGCCCGCACTTCCTGTCGATGCAACCTCCGACGAGGTAAGGAAGAACTTGATGGACATGTTCCGCGATCGACAAGCATTTTCCGAGCACACATGGAAGATGCTCCTGAGCGTGTGTCGGAGTTGGGCAGCATGGTGTAAGCTGAATAACAGAAAGTGGTTTCCCGCTGAACCCGAGGATGTTCGCGACTATCTTCTTTATCTCCAGGCACGGGGACTGGCTGTTAAGACTATTCAACAACATCTGGGGCAGCTGAACATGTTGCATCGAAGGTCTGGCCTGCCAAGACCATCAGATTCCAATGCTGTTTCACTTGTTATGAGGCGCATTCGGAAGGAAAACGTAGATGCAGGCGAACGCGCCAAGCAAGCCCTCGCATTCGAGCGGACCGATTTTGATCAGGTGAGATCCCTTATGGAAAACTCTGATCGGTGCCAGGATATAAGAAACCTGGCTTTCCTCGGCATAGCATACAACACCCTGCTTCGCATAGCAGAAATTGCTAGAATAAGGGTGAAGGACATCTCAAGAACCGATGGAGGCCGGATGCTGATACATATTGGAAGGACCAAGACTTTGGTGTCTACAGCAGGAGTTGAAAAAGCCCTTTCCCTGGGTGTTACCAAGCTGGTTGAACGGTGGATAAGTGTATCTGGGGTAGCAGACGACCCAAATAACTATCTTTTTTGTAGAGTACGGAAGAATGGTGTTGCAGCCCCATCTGCTACCAGCCAACTCTCCACAAGGGCACTTGAGGGGATATTTGAGGCCACCCACCGGCTCATATATGGGGCCAAAGACGACAGCGGCCAGAGATACCTTGCTTGGTCCGGCCACTCTGCCCGAGTTGGTGCAGCAAGGGATATGGCTCGCGCTGGCGTTTCTATACCCGAGATAATGCAAGCAGGGGGCTGGACAAATGTTAATATCGTAATGAATTATATCCGGAACCTTGACTCCGAGACTGGCGCTATGGTTCGACTTCTCGAAGATGGTGACTGAAGAGCAAGCTTTGAGACCAGATTGTTACTCCGACGGACGCAGTGGCTGACGGGCAGCTGTAGGGATAACAGGGTAATTCCTAATAAACTTACGCCCAA,3159,AACGATGATGCTCACTCTCGGGTCTCTAATGAGCAACTTGCTTACTGTTCATCAAAACCTGCCCGCACTTCCTGTCGATGCAACCTCCGACGAGGTAAGGAAGAACTTGATGGACATGTTCCGCGATCGACAAGCATTTTCCGAGCACACATGGAAGATGCTCCTGAGCGTGTGTCGGAGTTGGGCAGCATGGTGTAAGCTGAATAACAGAAAGTGGTTTCCCGCTGAACCCGAGGATGTTCGCGACTATCTTCTTTATCTCCAGGCACGGGGACTGGCTGTTAAGACTATTCAACAACATCTGGGGCAGCTGAACATGTTGCATCGAAGGTCTGGCCTGCCAAGACCATCAGATTCCAATGCTGTTTCACTTGTTATGAGGCGCATTCGGAAGGAAAACGTAGATGCAGGCGAACGCGCCAAGCAAGCCCTCGCATTCGAGCGGACCGATTTTGATCAGGTGAGATCCCTTATGGAAAACTCTGATCGGTGCCAGGATATAAGAAACCTGGCTTTCCTCGGCATAGCATACAACACCCTGCTTCGCATAGCAGAAATTGCTAGAATAAGGGTGAAGGACATCTCAAGAACCGATGGAGGCCGGATGCTGATACATATTGGAAGGACCAAGACTTTGGTGTCTACAGCAGGAGTTGAAAAAGCCCTTTCCCTGGGTGTTACCAAGCTGGTTGAACGGTGGATAAGTGTATCTGGGGTAGCAGACGACCCAAATAACTATCTTTTTTGTAGAGTACGGAAGAATGGTGTTGCAGCCCCATCTGCTACCAGCCAACTCTCCACAAGGGCACTTGAGGGGATATTTGAGGCCACCCACCGGCTCATATATGGGGCCAAAGACGACAGCGGCCAGAGATACCTTGCTTGGTCCGGCCACTCTGCCCGAGTTGGTGCAGCAAGGGATATGGCTCGCGCTGGCGTTTCTATACCCGAGATAATGCAAGCAGGGGGCTGGACAAATGTTAATATCGTAATGAATTATATCCGGAACCTTGACTCCGAGACTGGCGCTATGGTTCGACTTCTCGAAGATGGTGACTGAAGAGCAAGCTTTGAGACCAGATTGTTACTCCGACGGACGCAGTGGCTGACGGGCAGCTGTAGGGATAACAGGGTAATTCCTAATAAACTTACGCCCAA,1158,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000207.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/protein/YP_006472.1,,we are unaware,freegenes.github.io/genes/images/BBF10K_000207.png,,,,,7b9b6a13,False
2160,BBF10K_000210,FLP,S. cerevisiae cds FLP recombinase,Open Yeast Collection,pOpen-FLP,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000029654,,S. cerevisiae,yeast,For FLP-FRT recombinase,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGCCTCAGTTCGGCATACTCTGCAAGACTCCTCCCAAGGTTCTCGTCCGCCAATTTGTCGAGAGGTTTGAGAGGCCCAGTGGGGAAAAAATAGCCCTTTGTGCAGCCGAGCTCACATATCTTTGCTGGATGATCACACATAACGGAACAGCTATAAAAAGGGCCACTTTCATGTCCTATAACACTATTATTTCAAACTCACTCAGTTTCGACATTGTCAACAAAAGCCTCCAATTTAAGTATAAAACCCAGAAGGCCACCATTCTTGAAGCATCTCTGAAAAAACTGATACCCGCCTGGGAATTTACAATCATTCCATACTATGGGCAAAAGCATCAGAGTGACATTACTGACATCGTATCATCCTTGCAATTGCAATTCGAAAGTTCTGAGGAAGCTGATAAAGGCAACTCACATTCTAAAAAAATGCTTAAAGCCCTCCTCTCTGAAGGAGAGTCTATTTGGGAGATCACAGAAAAAATTTTGAACAGCTTTGAGTACACATCAAGATTCACTAAAACAAAGACCTTGTATCAGTTTCTGTTTCTCGCAACCTTCATTAACTGCGGGAGATTTTCCGATATAAAAAACGTTGACCCTAAATCCTTCAAGCTCGTCCAAAATAAGTACCTTGGAGTGATAATTCAATGCCTCGTAACAGAAACTAAGACAAGCGTATCTCGGCATATCTATTTTTTCTCCGCTAGAGGGCGAATCGATCCTTTGGTGTACTTGGATGAGTTCCTCAGAAATAGCGAACCAGTGCTGAAGAGAGTGAATCGAACCGGCAATTCCAGCAGCAATAAGCAAGAGTACCAGCTTCTGAAGGATAATTTGGTTAGGTCCTACAATAAAGCACTTAAGAAGAACGCCCCCTATTCTATATTCGCTATCAAAAATGGGCCCAAAAGTCATATCGGTCGACATCTTATGACCTCTTTTCTCAGTATGAAGGGATTGACCGAACTTACTAATGTAGTTGGTAACTGGAGTGATAAACGAGCATCAGCCGTCGCACGGACTACCTATACACACCAAATCACCGCCATCCCTGACCATTACTTTGCATTGGTGTCCAGATACTACGCATATGACCCAATTTCTAAAGAAATGATTGCCCTTAAAGACGAAACAAATCCAATAGAAGAATGGCAACACATAGAGCAGCTGAAGGGCTCCGCAGAGGGTAGCATCAGATACCCTGCTTGGAACGGTATAATCTCACAAGAAGTACTCGACTACCTCAGTTCCTACATAAACCGCAGAATCTGAAGAGCAAGCTTTGAGACCAGATTTCCGACGAGATTCCCTGCACATGCTTTAGACTCGTTTAGGGATAACAGGGTAATCAGGGGCAGGGGAATTTAACT,3399,AACGATGATGCTCACTCTCGGGTCTCTAATGCCTCAGTTCGGCATACTCTGCAAGACTCCTCCCAAGGTTCTCGTCCGCCAATTTGTCGAGAGGTTTGAGAGGCCCAGTGGGGAAAAAATAGCCCTTTGTGCAGCCGAGCTCACATATCTTTGCTGGATGATCACACATAACGGAACAGCTATAAAAAGGGCCACTTTCATGTCCTATAACACTATTATTTCAAACTCACTCAGTTTCGACATTGTCAACAAAAGCCTCCAATTTAAGTATAAAACCCAGAAGGCCACCATTCTTGAAGCATCTCTGAAAAAACTGATACCCGCCTGGGAATTTACAATCATTCCATACTATGGGCAAAAGCATCAGAGTGACATTACTGACATCGTATCATCCTTGCAATTGCAATTCGAAAGTTCTGAGGAAGCTGATAAAGGCAACTCACATTCTAAAAAAATGCTTAAAGCCCTCCTCTCTGAAGGAGAGTCTATTTGGGAGATCACAGAAAAAATTTTGAACAGCTTTGAGTACACATCAAGATTCACTAAAACAAAGACCTTGTATCAGTTTCTGTTTCTCGCAACCTTCATTAACTGCGGGAGATTTTCCGATATAAAAAACGTTGACCCTAAATCCTTCAAGCTCGTCCAAAATAAGTACCTTGGAGTGATAATTCAATGCCTCGTAACAGAAACTAAGACAAGCGTATCTCGGCATATCTATTTTTTCTCCGCTAGAGGGCGAATCGATCCTTTGGTGTACTTGGATGAGTTCCTCAGAAATAGCGAACCAGTGCTGAAGAGAGTGAATCGAACCGGCAATTCCAGCAGCAATAAGCAAGAGTACCAGCTTCTGAAGGATAATTTGGTTAGGTCCTACAATAAAGCACTTAAGAAGAACGCCCCCTATTCTATATTCGCTATCAAAAATGGGCCCAAAAGTCATATCGGTCGACATCTTATGACCTCTTTTCTCAGTATGAAGGGATTGACCGAACTTACTAATGTAGTTGGTAACTGGAGTGATAAACGAGCATCAGCCGTCGCACGGACTACCTATACACACCAAATCACCGCCATCCCTGACCATTACTTTGCATTGGTGTCCAGATACTACGCATATGACCCAATTTCTAAAGAAATGATTGCCCTTAAAGACGAAACAAATCCAATAGAAGAATGGCAACACATAGAGCAGCTGAAGGGCTCCGCAGAGGGTAGCATCAGATACCCTGCTTGGAACGGTATAATCTCACAAGAAGTACTCGACTACCTCAGTTCCTACATAAACCGCAGAATCTGAAGAGCAAGCTTTGAGACCAGATTTCCGACGAGATTCCCTGCACATGCTTTAGACTCGTTTAGGGATAACAGGGTAATCAGGGGCAGGGGAATTTAACT,1398,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000210.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/7559444-cleavage-dependent-ligation-by-the-flp-recombinase-characterization-of-a-mutant-flp-protein-with-an-alteration-in-a-catalytic-amino-acid/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000210.png,,,,,faa7c37d,False
2161,BBF10K_000211,GPP synthase,S. cerevisiae cds GPP synthase,Open Yeast Collection,pOpen-GPP-synthase,pBuild,M13 For,M13 Rev,,,,,,,Mentha x piperita,yeast,Geranyl pyrophosphate (GPP) synthase produces geranyl pyrophosphate (GPP) from DMAPP and IPP. GPP is the universal C10 precursor of the monoterpenes.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGCTATCAACTTGTCTCACATCAACTCTAAGACTTGTTTCCCATTAAAGACTAGATCAGACTTGTCAAGATCATCTTCTGCTAGATGTATGCCAACTGCTGCTGCTGCTGCTTTCCCAACTATCGCTACTGCTGCTCAATCTCAACCATACTGGGCTGCTATCGAAGCTGACATCGAAAGATACTTGAAGAAGTCTATCACTATCAGACCACCAGAAACTGTTTTCGGTCCAATGCACCACTTGACTTTCGCTGCTCCAGCTACTGCTGCTTCTACTTTGTGTTTGGCTGCTTGTGAATTGGTTGGTGGTGACAGATCACAAGCTATGGCTGCTGCTGCTGCTATCCACTTGGTTCACGCTGCTGCTTACGTTCACGAACACTTGCCATTGACTGACGGTTCAAGACCAGTTTCTAAGCCAGCTATCCAACACAAGTACGGTCCAAACGTTGAATTGTTGACTGGTGACGGTATCGTTCCATTCGGTTTCGAATTGTTGGCTGGTTCTGTTGACCCAGCTAGAACTGACGACCCAGACAGAATCTTGAGAGTTATCATCGAAATCTCAAGAGCTGGTGGTCCAGAAGGTATGATCTCTGGTTTGCACAGAGAAGAAGAAATCGTTGACGGTAACACTTCTTTGGACTTCATCGAATACGTTTGTAAGAAGAAGTACGGTGAAATGCACGCTTGTGGTGCTGCTTGTGGTGCTATCTTGGGTGGTGCTGCTGAAGAAGAAATCCAAAAGTTGAGAAACTTCGGTTTGTACCAAGGTACTTTGAGAGGTATGATGGAAATGAAGAACTCTCACCAATTGATCGACGAAAACATCATCGGTAAGTTGAAGGAATTGGCTTTGGAAGAATTGGGTGGTTTCCACGGTAAGAACGCTGAATTGATGTCATCTTTGGTTGCTGAACCATCTTTGTACGCTGCTTGAAGAGCAAGCTTTGAGACCAGCATCCGTCTAAATCTCGGCTTGGAGCGTATCGTTCGGATTAGGGATAACAGGGTAATCGCTGTCCTCGCACGTCCCAC,3069,AACGATGATGCTCACTCTCGGGTCTCTAATGGCTATCAACTTGTCTCACATCAACTCTAAGACTTGTTTCCCATTAAAGACTAGATCAGACTTGTCAAGATCATCTTCTGCTAGATGTATGCCAACTGCTGCTGCTGCTGCTTTCCCAACTATCGCTACTGCTGCTCAATCTCAACCATACTGGGCTGCTATCGAAGCTGACATCGAAAGATACTTGAAGAAGTCTATCACTATCAGACCACCAGAAACTGTTTTCGGTCCAATGCACCACTTGACTTTCGCTGCTCCAGCTACTGCTGCTTCTACTTTGTGTTTGGCTGCTTGTGAATTGGTTGGTGGTGACAGATCACAAGCTATGGCTGCTGCTGCTGCTATCCACTTGGTTCACGCTGCTGCTTACGTTCACGAACACTTGCCATTGACTGACGGTTCAAGACCAGTTTCTAAGCCAGCTATCCAACACAAGTACGGTCCAAACGTTGAATTGTTGACTGGTGACGGTATCGTTCCATTCGGTTTCGAATTGTTGGCTGGTTCTGTTGACCCAGCTAGAACTGACGACCCAGACAGAATCTTGAGAGTTATCATCGAAATCTCAAGAGCTGGTGGTCCAGAAGGTATGATCTCTGGTTTGCACAGAGAAGAAGAAATCGTTGACGGTAACACTTCTTTGGACTTCATCGAATACGTTTGTAAGAAGAAGTACGGTGAAATGCACGCTTGTGGTGCTGCTTGTGGTGCTATCTTGGGTGGTGCTGCTGAAGAAGAAATCCAAAAGTTGAGAAACTTCGGTTTGTACCAAGGTACTTTGAGAGGTATGATGGAAATGAAGAACTCTCACCAATTGATCGACGAAAACATCATCGGTAAGTTGAAGGAATTGGCTTTGGAAGAATTGGGTGGTTTCCACGGTAAGAACGCTGAATTGATGTCATCTTTGGTTGCTGAACCATCTTTGTACGCTGCTTGAAGAGCAAGCTTTGAGACCAGCATCCGTCTAAATCTCGGCTTGGAGCGTATCGTTCGGATTAGGGATAACAGGGTAATCGCTGTCCTCGCACGTCCCAC,1068,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000211.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC23900/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000211.png,,,,,75d5f6a0,False
2162,BBF10K_000217,GGPP synthase,S. cerevisiae cds GGPP synthase,Open Yeast Collection,pOpen-GGPP-synthase,pBuild,M13 For,M13 Rev,,,,,,,Pantoea ananatis,yeast,Geranylgeranyl diphosphate (GGPP) synthase catalyzes the synthesis of Geranylgeranyl diphosphate (GGPP) from farnesyl diphosphate and isopentenyl diphosphate.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGACTGTTTGTGCTAAGAAGCACGTTCACTTGACTAGAGACGCTGCTGAACAATTGTTGGCTGACATCGACAGAAGATTGGACCAATTGTTGCCAGTTGAAGGTGAAAGAGACGTTGTTGGTGCTGCTATGAGAGAAGGTGCTTTGGCTCCAGGTAAGAGAATCAGACCAATGTTGTTGTTGTTGACTGCTAGAGACTTGGGTTGTGCTGTTTCTCACGACGGTTTGTTGGACTTGGCTTGTGCTGTTGAAATGGTTCACGCTGCTTCTTTGATCTTGGACGACATGCCATGTATGGACGACGCTAAGTTGAGAAGAGGTAGACCAACTATCCACTCTCACTACGGTGAACACGTTGCTATCTTGGCTGCTGTTGCTTTGTTGTCTAAGGCTTTCGGTGTTATCGCTGACGCTGACGGTTTGACTCCATTGGCTAAGAACAGAGCTGTTTCTGAATTGTCTAACGCTATCGGTATGCAAGGTTTGGTTCAAGGTCAATTCAAGGACTTGTCTGAAGGTGACAAGCCAAGATCAGCTGAAGCTATCTTGATGACTAACCACTTCAAGACTTCTACTTTGTTCTGTGCTTCTATGCAAATGGCTTCTATCGTTGCTAACGCTTCTTCTGAAGCTAGAGACTGTTTGCACAGATTCTCTTTGGACTTGGGTCAAGCATTCCAATTGTTGGACGACTTGACTGACGGTATGACTGACACTGGTAAGGACTCTAACCAAGACGCTGGTAAGTCTACTTTGGTTAACTTGTTGGGTCCAAGAGCTGTTGAAGAAAGATTGAGACAACACTTGCAATTGGCTTCTGAACACTTGTCTGCTGCTTGTCAACACGGTCACGCTACTCAACACTTCATCCAAGCATGGTTCGACAAGAAGTTGGCTGCTGTTTCTTGAAGAGCAAGCTTTGAGACCAGCTATAAGAATTGCCGGGCACTCCCAGTGCCAAGGTCGGTTAGGGATAACAGGGTAATGGTGGACATACTAACTTTCTC,3036,AACGATGATGCTCACTCTCGGGTCTCTAATGACTGTTTGTGCTAAGAAGCACGTTCACTTGACTAGAGACGCTGCTGAACAATTGTTGGCTGACATCGACAGAAGATTGGACCAATTGTTGCCAGTTGAAGGTGAAAGAGACGTTGTTGGTGCTGCTATGAGAGAAGGTGCTTTGGCTCCAGGTAAGAGAATCAGACCAATGTTGTTGTTGTTGACTGCTAGAGACTTGGGTTGTGCTGTTTCTCACGACGGTTTGTTGGACTTGGCTTGTGCTGTTGAAATGGTTCACGCTGCTTCTTTGATCTTGGACGACATGCCATGTATGGACGACGCTAAGTTGAGAAGAGGTAGACCAACTATCCACTCTCACTACGGTGAACACGTTGCTATCTTGGCTGCTGTTGCTTTGTTGTCTAAGGCTTTCGGTGTTATCGCTGACGCTGACGGTTTGACTCCATTGGCTAAGAACAGAGCTGTTTCTGAATTGTCTAACGCTATCGGTATGCAAGGTTTGGTTCAAGGTCAATTCAAGGACTTGTCTGAAGGTGACAAGCCAAGATCAGCTGAAGCTATCTTGATGACTAACCACTTCAAGACTTCTACTTTGTTCTGTGCTTCTATGCAAATGGCTTCTATCGTTGCTAACGCTTCTTCTGAAGCTAGAGACTGTTTGCACAGATTCTCTTTGGACTTGGGTCAAGCATTCCAATTGTTGGACGACTTGACTGACGGTATGACTGACACTGGTAAGGACTCTAACCAAGACGCTGGTAAGTCTACTTTGGTTAACTTGTTGGGTCCAAGAGCTGTTGAAGAAAGATTGAGACAACACTTGCAATTGGCTTCTGAACACTTGTCTGCTGCTTGTCAACACGGTCACGCTACTCAACACTTCATCCAAGCATGGTTCGACAAGAAGTTGGCTGCTGTTTCTTGAAGAGCAAGCTTTGAGACCAGCTATAAGAATTGCCGGGCACTCCCAGTGCCAAGGTCGGTTAGGGATAACAGGGTAATGGTGGACATACTAACTTTCTC,1035,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000217.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC49583/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000217.png,,,,,52d8d369,False
2163,BBF10K_000227,FPP synthase,S. cerevisiae cds FPP synthase,Open Yeast Collection,pOpen-FPP-synthase,pBuild,M13 For,M13 Rev,,,,,,https://www.uniprot.org/uniprot/O64905,Helianthus annuus,yeast,Farnesyl pyrophosphate synthase,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGCTTCTGACTTGAAGTCTAAGTTCTTGCACGTTTACCAAACTTTGAAGTCTGAATTGTTGAACGACCCAGCTTTCGAGTTCCACCACGACTCAAGACAATGGATCGACAAGATGTTGGACTACAACGTTCCAGGTGGTAAGTTGAACAGAGGTTTGTCTGTTGTAGACTCTTACCAGTTGTTGAAGGGTGCTGAATTGACTGACGACGAAATCTTCTTGGCTTCTGCTTTGGGTTGGTGTATCGAATGGTTGCAAGCATACTTCTTGGTTTTGGACGACATCATGGACGGTTCTCACACTAGAAGAGGACAACCATGTTGGTTCAGATTGCCAAAGGTTGGTATGATCGCTGCTAACGACGGTTTGATCTTGAGAAACCACGTTCCAAGAATCTTGAAGAAGCACTTCAGAGGTAAGCCATACTACGTAGACTTGGTTGACTTGTTCAACGAAGTTGAGTTCCAAACTGCTTCTGGTCAAATGATCGACTTGATCACTACTTTGGTTGGTGAAAAGGACTTGTCTAAGTACTCTTTGTCTATCCACAGAAGAATCGTTCAATACAAGACTGCTTACTACTCTTTCTACTTGCCAGTTGCTTGTGCTTTGTTGATGTTCGGTGAGGACTTGGACAACCACGTTGAAGTTAAGAACGTTTTGGTTGAAATGGGTACTTACTTCCAAGTTCAAGACGACTACTTGGACTGTTTCGGTGCTCCAGAAGTTATCGGTAAGATCGGTACTGACATCGAGGACTTCTCTTCTTGGTTGGTTGTTAAGGCTTTGGAATTGGCTAACGAAGAACAAAAGAAGGTTTTGCACGAAAACTACGGTAAGAAGGACCCATCTTCTGTTGCTAAGGTTAAGGAATTGTACAACACTTTGAACTTGCAAGGTGTTTTCGAGGACTACGAATCTACTTCTTACAAGAAGTTGATCACTTCTATCGAAGGTCACCCATCTAAGGCTGTTCAAGCTGTTTTGAAGTCTTTCTTGGGTAAAATCTACAAGAGACAAAAGTGAAGAGCAAGCTTTGAGACCAGCTATGATCCCGGTGTAACGAGATGCTACTCAGACGTCCATAGGGATAACAGGGTAATTAGAGGAACATACGGCAGATC,3153,AACGATGATGCTCACTCTCGGGTCTCTAATGGCTTCTGACTTGAAGTCTAAGTTCTTGCACGTTTACCAAACTTTGAAGTCTGAATTGTTGAACGACCCAGCTTTCGAGTTCCACCACGACTCAAGACAATGGATCGACAAGATGTTGGACTACAACGTTCCAGGTGGTAAGTTGAACAGAGGTTTGTCTGTTGTAGACTCTTACCAGTTGTTGAAGGGTGCTGAATTGACTGACGACGAAATCTTCTTGGCTTCTGCTTTGGGTTGGTGTATCGAATGGTTGCAAGCATACTTCTTGGTTTTGGACGACATCATGGACGGTTCTCACACTAGAAGAGGACAACCATGTTGGTTCAGATTGCCAAAGGTTGGTATGATCGCTGCTAACGACGGTTTGATCTTGAGAAACCACGTTCCAAGAATCTTGAAGAAGCACTTCAGAGGTAAGCCATACTACGTAGACTTGGTTGACTTGTTCAACGAAGTTGAGTTCCAAACTGCTTCTGGTCAAATGATCGACTTGATCACTACTTTGGTTGGTGAAAAGGACTTGTCTAAGTACTCTTTGTCTATCCACAGAAGAATCGTTCAATACAAGACTGCTTACTACTCTTTCTACTTGCCAGTTGCTTGTGCTTTGTTGATGTTCGGTGAGGACTTGGACAACCACGTTGAAGTTAAGAACGTTTTGGTTGAAATGGGTACTTACTTCCAAGTTCAAGACGACTACTTGGACTGTTTCGGTGCTCCAGAAGTTATCGGTAAGATCGGTACTGACATCGAGGACTTCTCTTCTTGGTTGGTTGTTAAGGCTTTGGAATTGGCTAACGAAGAACAAAAGAAGGTTTTGCACGAAAACTACGGTAAGAAGGACCCATCTTCTGTTGCTAAGGTTAAGGAATTGTACAACACTTTGAACTTGCAAGGTGTTTTCGAGGACTACGAATCTACTTCTTACAAGAAGTTGATCACTTCTATCGAAGGTCACCCATCTAAGGCTGTTCAAGCTGTTTTGAAGTCTTTCTTGGGTAAAATCTACAAGAGACAAAAGTGAAGAGCAAGCTTTGAGACCAGCTATGATCCCGGTGTAACGAGATGCTACTCAGACGTCCATAGGGATAACAGGGTAATTAGAGGAACATACGGCAGATC,1152,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000227.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/7858223,,we are unaware,freegenes.github.io/genes/images/BBF10K_000227.png,,,,,5b2530bb,False
2164,BBF10K_000238,4S-limonene-synthase,S. cerevisiae CDS 4S-limonene-synthase,Open Yeast Collection,pOpen-4S-limonene-synthase,pBuild,M13 For,M13 Rev,,,,,,,Mentha spicata,yeast,"4S-limonene-synthase (EC 4.2.3.16) catalyzes the cyclization of geranyl diphosphate to (‚àí)-(4S)-limonene. Limonene is a relatively stable colorless liquid aliphatic hydrocarbon classified as a cyclic monoterpene, and is the is a major component of the aromatic scents such as in the oil of citrus fruit peels.","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGCTTTAAAAGTTTTGTCTGTTGCTACACAAATGGCTATACCATCAAATCTTACTACTTGTTTGCAACCATCTCATTTTAAATCTTCTCCAAAGCTATTGTCTAGTACTAACTCTTCTAGTAGATCGAGATTGAGAGTTTATTGTTCATCATCTCAATTAACTACTGAAAGGAGATCGGGTAATTATAATCCATCTAGGTGGGACGTTAATTTTATCCAATCTTTATTGAGTGACTATAAAGAGGATAAGCATGTTATTAGAGCTAGTGAGTTGGTTACTTTGGTTAAGATGGAATTAGAAAAAGAAACTGACCAAATTAGACAGTTGGAACTAATCGATGACTTGCAACGTATGGGTTTGTCAGATCATTTTCAAAATGAATTTAAGGAAATCTTGTCATCTATTTACTTAGACCATCATTATTATAAGAATCCATTCCCAAAGGAAGAAAGGGACTTGTATTCTACGTCTTTGGCATTTCGCTTGTTGAGAGAACATGGTTTTCAAGTTGCTCAAGAAGTCTTTGACTCTTTTAAGAACGAAGAAGGCGAATTTAAGGAGTCTTTATCTGACGATACTAGAGGTTTGTTGCAATTATATGAAGCTAGTTTCTTGTTAACTGAAGGTGAAACTACTTTAGAATCTGCTAGGGAATTTGCTACTAAATTTTTAGAAGAAAAGGTAAACGAAGGTGGTGTCGACGGTGATTTGCTTACTAGGATAGCTTATTCTTTAGACATTCCATTGCACTGGAGAATTAAAGGTCCAAATGCTCCAGTCTGGATTGAATGGTACAGAAAGAGGCCAGATATGAATCCAGTTGTTTTGGAGTTGGCTATTTTGGACTTGAATATAGTTCAAGCACAATTCCAAGAAGAATTGAAAGAATCTTTTCGATGGTGGAGAAATACTGGTTTTGTTGAAAAATTGCCTTTTGCTAGAGACAGATTGGTTGAGTGTTATTTCTGGAACACTGGTATTATTGAACCAAGACAACACGCTAGTGCTAGAATTATGATGGGTAAAGTTAATGCTTTGATTACTGTTATAGACGATATTTACGATGTTTATGGTACCTTGGAGGAATTGGAACAATTCACTGACTTGATAAGACGTTGGGATATTAACTCTATTGACCAATTGCCAGATTACATGCAATTGTGTTTTTTGGCTTTGAATAATTTCGTTGATGATACTTCTTATGACGTTATGAAGGAAAAAGGTGTTAATGTTATCCCATATTTGAGACAATCTTGGGTTGACCTAGCTGATAAATATATGGTTGAAGCTCGTTGGTTTTACGGTGGTCACAAGCCATCTCTCGAAGAATATTTGGAAAATTCTTGGCAATCTATCTCTGGTCCATGTATGTTGACACATATTTTTTTTAGGGTTACTGATTCATTCACTAAGGAAACTGTTGATTCCTTGTATAAATACCATGATTTAGTTAGATGGTCATCTTTCGTTTTGAGATTGGCTGATGATTTGGGTACTTCTGTTGAAGAAGTTTCAAGAGGTGACGTTCCAAAGTCTTTGCAATGTTACATGTCTGATTACAATGCTTCTGAAGCAGAAGCTAGAAAACATGTTAAGTGGTTGATTGCTGAAGTTTGGAAGAAGATGAATGCTGAAAGAGTTTCTAAGGATAGTCCATTTGGTAAGGATTTCATAGGTTGTGCTGTTGACTTGGGTAGAATGGCTCAATTGATGTATCACAATGGTGACGGTCACGGTACTCAACACCCAATTATCCACCAACAAATGACTAGAACTTTGTTCGAACCATTTGCTTGAAGAGCAAGCTTTGAGACCAGCTCAATCTAACAGTGGGGTTTAGAGGTCCAGACGGGGCCTAGGGATAACAGGGTAATCCCAGACCGCGCAAC,3921,AACGATGATGCTCACTCTCGGGTCTCTAATGGCTTTAAAAGTTTTGTCTGTTGCTACACAAATGGCTATACCATCAAATCTTACTACTTGTTTGCAACCATCTCATTTTAAATCTTCTCCAAAGCTATTGTCTAGTACTAACTCTTCTAGTAGATCGAGATTGAGAGTTTATTGTTCATCATCTCAATTAACTACTGAAAGGAGATCGGGTAATTATAATCCATCTAGGTGGGACGTTAATTTTATCCAATCTTTATTGAGTGACTATAAAGAGGATAAGCATGTTATTAGAGCTAGTGAGTTGGTTACTTTGGTTAAGATGGAATTAGAAAAAGAAACTGACCAAATTAGACAGTTGGAACTAATCGATGACTTGCAACGTATGGGTTTGTCAGATCATTTTCAAAATGAATTTAAGGAAATCTTGTCATCTATTTACTTAGACCATCATTATTATAAGAATCCATTCCCAAAGGAAGAAAGGGACTTGTATTCTACGTCTTTGGCATTTCGCTTGTTGAGAGAACATGGTTTTCAAGTTGCTCAAGAAGTCTTTGACTCTTTTAAGAACGAAGAAGGCGAATTTAAGGAGTCTTTATCTGACGATACTAGAGGTTTGTTGCAATTATATGAAGCTAGTTTCTTGTTAACTGAAGGTGAAACTACTTTAGAATCTGCTAGGGAATTTGCTACTAAATTTTTAGAAGAAAAGGTAAACGAAGGTGGTGTCGACGGTGATTTGCTTACTAGGATAGCTTATTCTTTAGACATTCCATTGCACTGGAGAATTAAAGGTCCAAATGCTCCAGTCTGGATTGAATGGTACAGAAAGAGGCCAGATATGAATCCAGTTGTTTTGGAGTTGGCTATTTTGGACTTGAATATAGTTCAAGCACAATTCCAAGAAGAATTGAAAGAATCTTTTCGATGGTGGAGAAATACTGGTTTTGTTGAAAAATTGCCTTTTGCTAGAGACAGATTGGTTGAGTGTTATTTCTGGAACACTGGTATTATTGAACCAAGACAACACGCTAGTGCTAGAATTATGATGGGTAAAGTTAATGCTTTGATTACTGTTATAGACGATATTTACGATGTTTATGGTACCTTGGAGGAATTGGAACAATTCACTGACTTGATAAGACGTTGGGATATTAACTCTATTGACCAATTGCCAGATTACATGCAATTGTGTTTTTTGGCTTTGAATAATTTCGTTGATGATACTTCTTATGACGTTATGAAGGAAAAAGGTGTTAATGTTATCCCATATTTGAGACAATCTTGGGTTGACCTAGCTGATAAATATATGGTTGAAGCTCGTTGGTTTTACGGTGGTCACAAGCCATCTCTCGAAGAATATTTGGAAAATTCTTGGCAATCTATCTCTGGTCCATGTATGTTGACACATATTTTTTTTAGGGTTACTGATTCATTCACTAAGGAAACTGTTGATTCCTTGTATAAATACCATGATTTAGTTAGATGGTCATCTTTCGTTTTGAGATTGGCTGATGATTTGGGTACTTCTGTTGAAGAAGTTTCAAGAGGTGACGTTCCAAAGTCTTTGCAATGTTACATGTCTGATTACAATGCTTCTGAAGCAGAAGCTAGAAAACATGTTAAGTGGTTGATTGCTGAAGTTTGGAAGAAGATGAATGCTGAAAGAGTTTCTAAGGATAGTCCATTTGGTAAGGATTTCATAGGTTGTGCTGTTGACTTGGGTAGAATGGCTCAATTGATGTATCACAATGGTGACGGTCACGGTACTCAACACCCAATTATCCACCAACAAATGACTAGAACTTTGTTCGAACCATTTGCTTGAAGAGCAAGCTTTGAGACCAGCTCAATCTAACAGTGGGGTTTAGAGGTCCAGACGGGGCCTAGGGATAACAGGGTAATCCCAGACCGCGCAAC,1920,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000238.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/8226816-4s-limonene-synthase-from-the-oil-glands-of-spearmint-mentha-spicata-cdna-isolation-characterization-and-bacterial-expression-of-the-catalytically-active-monoterpene-cyclase/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000238.png,,,,,2585680b,False
2165,BBF10K_000243,limonene-3-hydroxylase,S. cerevisiae cds limonene-3-hydroxylase,Open Yeast Collection,pOpen-limonene-3-hydroxylase,pBuild,M13 For,M13 Rev,,,,,,,Mentha spicata,yeast,"(‚àí)-Limonene-3-hydroxylase (L3OH), using O2 and NADPH, catalyzes the allylic hydroxylation of (‚àí)-limonene at the 3 position to (‚àí)-trans-isopiperitenol.","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGAATTGCAAATATCTTCCGCTATTATTATTTTGGTTGCTACATTTGTTGCTTCTTTGTTGATTAAGCAATGGAGAAAGAGTGAATCTAGGCAAAATTTGCCACCAGGTCCACCAAAGTTACCATTGGTTGGTCATTTGCATTTATTGTGGGGTAAATTGCCACAACATGCTATGGCCGATATGGCAAAAAAATATGGTCCAGTTACTCATGTTCAATTGGGTGAAGTATTTTCTGTAGTTTTGTCATCTAGGGAAGCTACTAAGGAAGCCATGAAATTGTTAGATCCAGCATGTGCTGATAGGTTCGAATCTATTGGTACTAGAATTATGTGGTATGATAATGATGACATTATATTCTCTCCATATTCTGATCATTGGAGACAAATGAGGAAAATCTGTGTTTCTGAATTATTGTCTGCTAGAAATGTTAGGTCATTCGGATTTATTAGACAAGACGAAATGTCAAGATTGTTGAGACACTTGCAATCTTCTGCCGGTGAAACTGTTGATATGACTGAAAGAATAGCTACTTTAACTTGTAGTATTATCTGTAGAGCAGCTTTTGGTGCTATCATTAACGATCACGAAGAATTAGTTGAATTGGTTAAAGACTCTTTGTCAATGGCTAGTGGTTTCGAATTGGCTGATTTGTTTCCATCTTCTAAATTATTAAACTTGCTATGTTGGAATAAGTCTAAGTTGTGGAGAATGAGAAGAAGAGTTGATACTATATTGGAAGCTATAGTCGACGAACATAAATTAAAAAAGTCTGGTGAGTTCGGTGGTGAAGATATAATAGATGTTTTGTTTAGGATGCAAAAGGATTCTCAAATTAAGGTTCCTATTACTACTAACGCTATAAAAGCGTTCATTTTTGACACTTTTTCTGCTGGAACTGAAACTAGCTCTACTACGACTTTGTGGGTCATGGCTGAATTGATGAGAAATCCAGCTGTTATGGCTAAAGCTCAAGCTGAGGTTAGAGCTGCTTTGAAAGGTAAAACTTCTGTTGACGTTGACGACGTTCAAGAATTGAAATATATGAAGAGTGTTGTTAAGGAAACTATGAGAATGCACCCACCAATCCCACTAATACCAAGATCATGTAGAGAGGAATGTGAAGTTAATGGTTATAAAATTCCAAATAAAGCTAGAATCATGATCAATGTCTGGTCTATGGGTAGAAACCCATTGTATTGGGAAAAACCAGAAACTTTTTGGCCAGAACGTTTTGATCAAGTTTCAAGAGATTTTATGGGTTCTGATTTTGAATTTATACCATTCGGTGCTGGTAGAAGAATCTGTCCAGGTTTAAATTTCGGTTTGGCTAATGTTGAAGTTCCATTGGCTCAATTGTTGTATCATTTCGACTGGAAATTGGCCGAAGGTATGAAACCATCTGATATGGATATGTCTGAAGCTGAAGGTTTGACTGGTATCAGAAAGAACAACTTGTTGTTGGTTCCAACTTTGTACAAGTCTCCATGAAGAGCAAGCTTTGAGACCAGGACACCAGACCAATGAAGACTCGTATCGAAACAGGGTTTTAGGGATAACAGGGTAATCCCGTTAAGGCAGGGCATGGG,3618,AACGATGATGCTCACTCTCGGGTCTCTAATGGAATTGCAAATATCTTCCGCTATTATTATTTTGGTTGCTACATTTGTTGCTTCTTTGTTGATTAAGCAATGGAGAAAGAGTGAATCTAGGCAAAATTTGCCACCAGGTCCACCAAAGTTACCATTGGTTGGTCATTTGCATTTATTGTGGGGTAAATTGCCACAACATGCTATGGCCGATATGGCAAAAAAATATGGTCCAGTTACTCATGTTCAATTGGGTGAAGTATTTTCTGTAGTTTTGTCATCTAGGGAAGCTACTAAGGAAGCCATGAAATTGTTAGATCCAGCATGTGCTGATAGGTTCGAATCTATTGGTACTAGAATTATGTGGTATGATAATGATGACATTATATTCTCTCCATATTCTGATCATTGGAGACAAATGAGGAAAATCTGTGTTTCTGAATTATTGTCTGCTAGAAATGTTAGGTCATTCGGATTTATTAGACAAGACGAAATGTCAAGATTGTTGAGACACTTGCAATCTTCTGCCGGTGAAACTGTTGATATGACTGAAAGAATAGCTACTTTAACTTGTAGTATTATCTGTAGAGCAGCTTTTGGTGCTATCATTAACGATCACGAAGAATTAGTTGAATTGGTTAAAGACTCTTTGTCAATGGCTAGTGGTTTCGAATTGGCTGATTTGTTTCCATCTTCTAAATTATTAAACTTGCTATGTTGGAATAAGTCTAAGTTGTGGAGAATGAGAAGAAGAGTTGATACTATATTGGAAGCTATAGTCGACGAACATAAATTAAAAAAGTCTGGTGAGTTCGGTGGTGAAGATATAATAGATGTTTTGTTTAGGATGCAAAAGGATTCTCAAATTAAGGTTCCTATTACTACTAACGCTATAAAAGCGTTCATTTTTGACACTTTTTCTGCTGGAACTGAAACTAGCTCTACTACGACTTTGTGGGTCATGGCTGAATTGATGAGAAATCCAGCTGTTATGGCTAAAGCTCAAGCTGAGGTTAGAGCTGCTTTGAAAGGTAAAACTTCTGTTGACGTTGACGACGTTCAAGAATTGAAATATATGAAGAGTGTTGTTAAGGAAACTATGAGAATGCACCCACCAATCCCACTAATACCAAGATCATGTAGAGAGGAATGTGAAGTTAATGGTTATAAAATTCCAAATAAAGCTAGAATCATGATCAATGTCTGGTCTATGGGTAGAAACCCATTGTATTGGGAAAAACCAGAAACTTTTTGGCCAGAACGTTTTGATCAAGTTTCAAGAGATTTTATGGGTTCTGATTTTGAATTTATACCATTCGGTGCTGGTAGAAGAATCTGTCCAGGTTTAAATTTCGGTTTGGCTAATGTTGAAGTTCCATTGGCTCAATTGTTGTATCATTTCGACTGGAAATTGGCCGAAGGTATGAAACCATCTGATATGGATATGTCTGAAGCTGAAGGTTTGACTGGTATCAGAAAGAACAACTTGTTGTTGGTTCCAACTTTGTACAAGTCTCCATGAAGAGCAAGCTTTGAGACCAGGACACCAGACCAATGAAGACTCGTATCGAAACAGGGTTTTAGGGATAACAGGGTAATCCCGTTAAGGCAGGGCATGGG,1617,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000243.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/10415126,,we are unaware,freegenes.github.io/genes/images/BBF10K_000243.png,,,,,c74a4fb6,False
2166,BBF10K_000246,S-linalool synthase,S. cerevisiae cds LIS (S-linalool synthase),Open Yeast Collection,pOpen-LIS,pBuild,M13 For,M13 Rev,,,,,,https://www.uniprot.org/uniprot/Q96376,Clarkia breweri,yeast,Linalool synthase (LIS) produces (S)-linalool from geranyl diphosphate and water. Linalool is a naturally occurring terpene alcohol found in many flowers and spice plants.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGCAATTGATAACCAATTTTTCATCCTCCTCTAGTGAATTGCAGTTTTTAGTTGACAAAGTTAAAAGAGAATCTCTAAGTTCTTCCAGTTCTAATACTCAAAATTTATTCTTGAGTACTTCTCCCTATGATACTGCTTGGCTCGCTTTGATTCCACATCCTCACCATCATCACCACCATGGTAGACCAATGTTCGAAAAATGTTTGCAATGGATTTTGCATAATCAAACTCCACAGGGTTTTTGGGCTGCCGCTGGTGACAATATTTCAGATACAGACGATGACGTTACTTTAGACTGTTTGCTATCTACTCTAGCGTGTTTGGTTGCTTTGAAAAGATGGCAACTAGCTCCAGATATGATTCACAAGGGTCTGGAATTTGTTAATAGAAATACTGAAAGGTTGGTAATGAAACAAAAGCCCTCTGATGTTCCAAGATGGTTCACTATAATGTTCCCAGCTATGTTGGAGTTGGCAGGCGCTTCTTCACTAAGGGTAGATTTTTCTGAAAATTTGAATAGAATATTGGTTGAACTATCTCAGAACAGGGATGATATTCTTACTAGAGAGGAAGTAGACGAAAAGAAACAATACTCTCCATTGTTATTATTTTTGGAAGCATTGCCTGCTCAATCATACGATAACGATGTTTTGAAGCAAATTATTGACAAGAATCTATCTAATGACGGTTCTTTATTGCAATCTCCATCTGCTACTGCTCGTGCTTATATGATAACTGGCAACACTAGATGTTTATCTTATTTGCATTCATTGACGAACTCTTGTAGCAACGGTGGTGTTCCATCATTCTATCCAGTCGATGATGACTTGCATGACTTAGTTATGGTCAATCAATTAACTAGATCGGGTTTAACTGAACATTTGATCCCAGAAATAGACCACTTGTTACTTAAGGTTCAAAAAAACTATAAGTATAAGAAGGCTTCTCCAAAATCTTTGTACTCTATAGCTGCTGAATTGTATAGAGACTCTTTAGCTTTCTGGTTATTGAGAGTTAATAATCATTGGGTTAGTCCATCTATTTTTTGTTGGTTCTTGGATGACGACGAAATCAGAGATCACATTGAAACTAACTACGAAGAATTTGCAGCTGTTTTGCTAAATGTCTATAGGGCTACTGACCTTATGTTCTCTGGTGAAGTTCAGCTTGTTGAGGCTAGGTCATTTGCTACTAAGAACCTAGAAAATATATTGGCCACTGGTAATATACACAAGACTAATGCTGACATTTCATCTTCATTGCACAAAATGATAGAACACGAATTAAGAGTCCCATGGACTGCTAGAATGGATCACGTTGAGAACAGAATATGGATCGAAGAGATCGCTTCATCAGCTTTATGGTTTGGTAAATCTTCCTATTTGAGATTGTCATGTTTTCATAAGATGTCTCTTCAGCAATTAGCTGTTAAAAATTACACTTTGAGACAATTAGTTTATAGGGACGAATTGGCTGAAGTTGAGAGATGGTCTAAGGAAAGAGGTTTGTGTGATATGGGTTTCTGTAGAGAAAAGACTGGTTACTGTTACTATGCTTTTGCTGCATCTACTTGTCTGCCATGGTCTAGTGACGTTCGATTGGTATTGACTAAGGCTGCTGTTGTTATAACTGTTGCTGACGATTTCTTTGACGTCGAAGGTTCTATGGTTGATTTGGAAAAGTTGACTGATGCTGTTAGGAGATGGGACGCTGAAGGTTTGGGTAGTCACTCTAAGACTATCTTTGAGGCATTGGACGACTTGGTTAATGAAGTTAGATTGAAGTGTTTCCAACAGAACGGTCAAGATATTAAGAACAACTTACAACAATTGTGGTATGAAACTTTCCATTCATGGCTAATGGAAGCTAAGTGGGGTAAGGGTTTGACTTCCAAGCCATCTGTTGACGTTTACTTGGGTAATGCTATGACTTCTATTGCTGCTCATACTATGGTCCTAACTGCTTCCTGTTTATTGGGTCCAGGTTTCCCAGTTCACCAACTGTGGTCACAAAGACGTCACCAAGACATTACTTCTTTGTTGATGGTTTTGACTAGATTGTTGAATGACATACAATCTTACTTGAAGGAAGAAGATGAAGGTAAGATCAACTACGTTTGGATGTACATGATCGAAAACAATCAAGCTAGTATAGATGACTCTGTTAGACACGTTCAAACTATTATCAACGTAAAAAAGCAAGAATTTATCCAAAGAGTTTTGTCTGATCAACATTGTAACTTGCCAAAGTCTTTCAAGCAATTGCATTTCTCTTGTTTGAAGGTCTTTAACATGTTCTTCAACTCTTCTAACATATTTGATACCGATACTGACTTGTTGTTGGACATTCACGAAGCGTTCGTTTCTCCACCACAAGTTCCAAAGTTTAAACCTCATATCAAACCACCCCACCAATTGCCAGCTACTTTACAACCTCCACACCAACCACAACAAATAATGGTTAACAAGAAGAAGGTTGAAATGGTTTATAAGTCTTACCATCACCCATTCAAGGTTTTTACTTTGCAAAAGAAGCAATCTTCTGGTCATGGTACTATGAACCCAAGAGCTTCTATCTTGGCTGGTCCAAATATAAAATTGTGTTTTTCTTGAAGAGCAAGCTTTGAGACCAGGGCTAATTACCATCAGCGCTCCTCGTCAACATTATCTAGTAGGGATAACAGGGTAATGTACTAACCCGACCCATGGTG,4740,AACGATGATGCTCACTCTCGGGTCTCTAATGCAATTGATAACCAATTTTTCATCCTCCTCTAGTGAATTGCAGTTTTTAGTTGACAAAGTTAAAAGAGAATCTCTAAGTTCTTCCAGTTCTAATACTCAAAATTTATTCTTGAGTACTTCTCCCTATGATACTGCTTGGCTCGCTTTGATTCCACATCCTCACCATCATCACCACCATGGTAGACCAATGTTCGAAAAATGTTTGCAATGGATTTTGCATAATCAAACTCCACAGGGTTTTTGGGCTGCCGCTGGTGACAATATTTCAGATACAGACGATGACGTTACTTTAGACTGTTTGCTATCTACTCTAGCGTGTTTGGTTGCTTTGAAAAGATGGCAACTAGCTCCAGATATGATTCACAAGGGTCTGGAATTTGTTAATAGAAATACTGAAAGGTTGGTAATGAAACAAAAGCCCTCTGATGTTCCAAGATGGTTCACTATAATGTTCCCAGCTATGTTGGAGTTGGCAGGCGCTTCTTCACTAAGGGTAGATTTTTCTGAAAATTTGAATAGAATATTGGTTGAACTATCTCAGAACAGGGATGATATTCTTACTAGAGAGGAAGTAGACGAAAAGAAACAATACTCTCCATTGTTATTATTTTTGGAAGCATTGCCTGCTCAATCATACGATAACGATGTTTTGAAGCAAATTATTGACAAGAATCTATCTAATGACGGTTCTTTATTGCAATCTCCATCTGCTACTGCTCGTGCTTATATGATAACTGGCAACACTAGATGTTTATCTTATTTGCATTCATTGACGAACTCTTGTAGCAACGGTGGTGTTCCATCATTCTATCCAGTCGATGATGACTTGCATGACTTAGTTATGGTCAATCAATTAACTAGATCGGGTTTAACTGAACATTTGATCCCAGAAATAGACCACTTGTTACTTAAGGTTCAAAAAAACTATAAGTATAAGAAGGCTTCTCCAAAATCTTTGTACTCTATAGCTGCTGAATTGTATAGAGACTCTTTAGCTTTCTGGTTATTGAGAGTTAATAATCATTGGGTTAGTCCATCTATTTTTTGTTGGTTCTTGGATGACGACGAAATCAGAGATCACATTGAAACTAACTACGAAGAATTTGCAGCTGTTTTGCTAAATGTCTATAGGGCTACTGACCTTATGTTCTCTGGTGAAGTTCAGCTTGTTGAGGCTAGGTCATTTGCTACTAAGAACCTAGAAAATATATTGGCCACTGGTAATATACACAAGACTAATGCTGACATTTCATCTTCATTGCACAAAATGATAGAACACGAATTAAGAGTCCCATGGACTGCTAGAATGGATCACGTTGAGAACAGAATATGGATCGAAGAGATCGCTTCATCAGCTTTATGGTTTGGTAAATCTTCCTATTTGAGATTGTCATGTTTTCATAAGATGTCTCTTCAGCAATTAGCTGTTAAAAATTACACTTTGAGACAATTAGTTTATAGGGACGAATTGGCTGAAGTTGAGAGATGGTCTAAGGAAAGAGGTTTGTGTGATATGGGTTTCTGTAGAGAAAAGACTGGTTACTGTTACTATGCTTTTGCTGCATCTACTTGTCTGCCATGGTCTAGTGACGTTCGATTGGTATTGACTAAGGCTGCTGTTGTTATAACTGTTGCTGACGATTTCTTTGACGTCGAAGGTTCTATGGTTGATTTGGAAAAGTTGACTGATGCTGTTAGGAGATGGGACGCTGAAGGTTTGGGTAGTCACTCTAAGACTATCTTTGAGGCATTGGACGACTTGGTTAATGAAGTTAGATTGAAGTGTTTCCAACAGAACGGTCAAGATATTAAGAACAACTTACAACAATTGTGGTATGAAACTTTCCATTCATGGCTAATGGAAGCTAAGTGGGGTAAGGGTTTGACTTCCAAGCCATCTGTTGACGTTTACTTGGGTAATGCTATGACTTCTATTGCTGCTCATACTATGGTCCTAACTGCTTCCTGTTTATTGGGTCCAGGTTTCCCAGTTCACCAACTGTGGTCACAAAGACGTCACCAAGACATTACTTCTTTGTTGATGGTTTTGACTAGATTGTTGAATGACATACAATCTTACTTGAAGGAAGAAGATGAAGGTAAGATCAACTACGTTTGGATGTACATGATCGAAAACAATCAAGCTAGTATAGATGACTCTGTTAGACACGTTCAAACTATTATCAACGTAAAAAAGCAAGAATTTATCCAAAGAGTTTTGTCTGATCAACATTGTAACTTGCCAAAGTCTTTCAAGCAATTGCATTTCTCTTGTTTGAAGGTCTTTAACATGTTCTTCAACTCTTCTAACATATTTGATACCGATACTGACTTGTTGTTGGACATTCACGAAGCGTTCGTTTCTCCACCACAAGTTCCAAAGTTTAAACCTCATATCAAACCACCCCACCAATTGCCAGCTACTTTACAACCTCCACACCAACCACAACAAATAATGGTTAACAAGAAGAAGGTTGAAATGGTTTATAAGTCTTACCATCACCCATTCAAGGTTTTTACTTTGCAAAAGAAGCAATCTTCTGGTCATGGTACTATGAACCCAAGAGCTTCTATCTTGGCTGGTCCAAATATAAAATTGTGTTTTTCTTGAAGAGCAAGCTTTGAGACCAGGGCTAATTACCATCAGCGCTCCTCGTCAACATTATCTAGTAGGGATAACAGGGTAATGTACTAACCCGACCCATGGTG,2739,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000246.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/8768373,,we are unaware,freegenes.github.io/genes/images/BBF10K_000246.png,,,,,1aa9572e,False
2167,BBF10K_000256,carveol dehydrogenase,S. cerevisiae cds carveol dehydrogenase,Open Yeast Collection,pOpen-carveol-dehydrogenase,pBuild,M13 For,M13 Rev,,,,,,https://www.uniprot.org/uniprot/Q5C9I9,Mentha piperita,yeast,"(-)-isopiperitenol/(-)-carveol dehydrogenase is capable of utilizing (‚àí)-trans-isopiperitenol in peppermint and (‚àí)-trans-carveol in spearmint. Carveol is a natural unsaturated, monocyclic monoterpenoid alcohol that is a constituent of spearmint essential oil in the form of cis-(‚àí)-carveol.","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,AATG,GCTT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAATGGAATTGGACTTGTTGTCTGCTATCATCATCTTGGTTGCTACTTACATCGTTTCTTTGTTGATCAACCAATGGAGAAAGTCTAAGTCTCAACAAAACTTGCCACCATCTCCACCAAAGTTGCCAGTTATCGGTCACTTGCACTTCTTGTGGGGTGGTTTGCCACAACACGTTTTCAGATCAAACGCTCAAAAGTACGGTCCAGTTGCTCACGTTCAATTGGGTGAAGTTTACTCTGTTGTTTTGTCATCTGCTGAAGCTGCTAAGCAAGCTATGAAGGTTTTGGACCCAAACTTCGCTGACAGATTCGACGGTATCGGTTCAAGAACTATGTGGTACGACAAGGACGACATCATCTTCTCTCCATACAACGACCACTGGAGACAAATGAGAAGAATCTGTGTTACTGAATTGTTGTCTCCAAAGAACGTTAGATCATTCGGTTACATCAGACAAGAAGAAATCGAAAGATTGATCAGATTGTTGGGTTCTTCTGGTGGTGCTCCAGTTGACGTTACTGAAGAAGTTTCTAAGATGTCTTGTGTTGTTGTTTGTAGAGCTGCTTTCGGTTCTGTTTTGAAGGACCAAGGTTCTTTGGCTGAATTGGTTAAGGAATCTTTGGCTTTGGCTTCTGGTTTCGAATTGGCTGACTTGTACCCATCTTCTTGGTTGTTGAACTTGTTGTCTTTGAACAAGTACAGATTGCAAAGAATGAGAAGAAGATTGGACCACATCTTGGACGGTTTCTTGGAAGAACACAGAGAAAAGAAGTCTGGTGAGTTCGGTGGTGAAGATATCGTTGACGTTTTGTTCAGAATGCAAAAGGGTTCTGACATCAAGATCCCAATCACTTCTAACTGTATCAAGGGTTTCATCTTCGACACTTTCTCTGCTGGTGCTGAAACTTCTTCTACTACTATCTCTTGGGCTTTGTCTGAATTGATGAGAAACCCAGCTAAGATGGCTAAGGTTCAAGCTGAAGTTAGAGAAGCATTGAAGGGTAAGACTGTTGTTGACTTGTCTGAAGTTCAAGAATTGAAGTACTTGAGATCAGTTTTGAAGGAAACTTTGAGATTGCACCCACCATTCCCATTGATCCCAAGACAATCAAGAGAAGAATGTGAAGTTAACGGTTACACTATCCCAGCTAAGACTAGAATCTTCATCAACGTTTGGGCTATCGGTAGAGATCCACAATACTGGGAAGATCCAGACACTTTCAGACCAGAAAGATTCGACGAAGTTTCAAGAGACTTCATGGGTAACGACTTCGAGTTCATCCCATTCGGTGCTGGTAGAAGAATCTGTCCAGGTTTGCACTTCGGTTTGGCTAACGTTGAAATCCCATTGGCTCAATTGTTGTACCACTTCGACTGGAAGTTGCCACAAGGTATGACTGACGCTGACTTGGACATGACTGAAACTCCAGGTTTGTCTGGTCCAAAGAAGAAGAACGTTTGTTTGGTTCCAACTTTGTACAAGTCTCCATGAAGAGCAAGCTTTGAGACCAGGTGATCTGACGAATGTCCATATTCCCATCATCACCAGAATAGGGATAACAGGGTAATACCCTAGGCCGTAAGTTGATA,3618,AACGATGATGCTCACTCTCGGGTCTCTAATGGAATTGGACTTGTTGTCTGCTATCATCATCTTGGTTGCTACTTACATCGTTTCTTTGTTGATCAACCAATGGAGAAAGTCTAAGTCTCAACAAAACTTGCCACCATCTCCACCAAAGTTGCCAGTTATCGGTCACTTGCACTTCTTGTGGGGTGGTTTGCCACAACACGTTTTCAGATCAAACGCTCAAAAGTACGGTCCAGTTGCTCACGTTCAATTGGGTGAAGTTTACTCTGTTGTTTTGTCATCTGCTGAAGCTGCTAAGCAAGCTATGAAGGTTTTGGACCCAAACTTCGCTGACAGATTCGACGGTATCGGTTCAAGAACTATGTGGTACGACAAGGACGACATCATCTTCTCTCCATACAACGACCACTGGAGACAAATGAGAAGAATCTGTGTTACTGAATTGTTGTCTCCAAAGAACGTTAGATCATTCGGTTACATCAGACAAGAAGAAATCGAAAGATTGATCAGATTGTTGGGTTCTTCTGGTGGTGCTCCAGTTGACGTTACTGAAGAAGTTTCTAAGATGTCTTGTGTTGTTGTTTGTAGAGCTGCTTTCGGTTCTGTTTTGAAGGACCAAGGTTCTTTGGCTGAATTGGTTAAGGAATCTTTGGCTTTGGCTTCTGGTTTCGAATTGGCTGACTTGTACCCATCTTCTTGGTTGTTGAACTTGTTGTCTTTGAACAAGTACAGATTGCAAAGAATGAGAAGAAGATTGGACCACATCTTGGACGGTTTCTTGGAAGAACACAGAGAAAAGAAGTCTGGTGAGTTCGGTGGTGAAGATATCGTTGACGTTTTGTTCAGAATGCAAAAGGGTTCTGACATCAAGATCCCAATCACTTCTAACTGTATCAAGGGTTTCATCTTCGACACTTTCTCTGCTGGTGCTGAAACTTCTTCTACTACTATCTCTTGGGCTTTGTCTGAATTGATGAGAAACCCAGCTAAGATGGCTAAGGTTCAAGCTGAAGTTAGAGAAGCATTGAAGGGTAAGACTGTTGTTGACTTGTCTGAAGTTCAAGAATTGAAGTACTTGAGATCAGTTTTGAAGGAAACTTTGAGATTGCACCCACCATTCCCATTGATCCCAAGACAATCAAGAGAAGAATGTGAAGTTAACGGTTACACTATCCCAGCTAAGACTAGAATCTTCATCAACGTTTGGGCTATCGGTAGAGATCCACAATACTGGGAAGATCCAGACACTTTCAGACCAGAAAGATTCGACGAAGTTTCAAGAGACTTCATGGGTAACGACTTCGAGTTCATCCCATTCGGTGCTGGTAGAAGAATCTGTCCAGGTTTGCACTTCGGTTTGGCTAACGTTGAAATCCCATTGGCTCAATTGTTGTACCACTTCGACTGGAAGTTGCCACAAGGTATGACTGACGCTGACTTGGACATGACTGAAACTCCAGGTTTGTCTGGTCCAAAGAAGAAGAACGTTTGTTTGGTTCCAACTTTGTACAAGTCTCCATGAAGAGCAAGCTTTGAGACCAGGTGATCTGACGAATGTCCATATTCCCATCATCACCAGAATAGGGATAACAGGGTAATACCCTAGGCCGTAAGTTGATA,1617,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000256.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/2297225,,we are unaware,freegenes.github.io/genes/images/BBF10K_000256.png,,,,,2a3a0690,False
2169,BBF10K_000327,ScGal4-DNA-AD,S. cerevisiae cds-Gal4-DNA-Activation-Domain,Open Yeast Collection,pOpen-ScGal4-DNA-AD,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000006169,,S. cerevisiae,S. cerevisiae,Yeast-2-hybrid bait fusion - Gal4-DNA-Activation-Domain,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,TCCAtg,ggAATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTTCCATGGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAGGGAATGTGAGACCAGTAGTATCCGAATCGCTGCGAGTAATACCATGGCACAACGTAGGGATAACAGGGTAATCATGGCTAGGGCATCCAACTG,2469,AACGATGATGCTCACTCTCGGGTCTCTTCCATGGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAGGGAATGTGAGACCAGTAGTATCCGAATCGCTGCGAGTAATACCATGGCACAACGTAGGGATAACAGGGTAATCATGGCTAGGGCATCCAACTG,468,ColE1 High Copy,500-701,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000327.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/2547163,,we are unaware,freegenes.github.io/genes/images/BBF10K_000327.png,,,,,42ecdf0a,False
2170,BBF10K_000329,ScGal4-DNA-BD,S. cerevisiae cds Gal4 DNA-Binding-Domain,Open Yeast Collection,pOpen-ScGal4-DNA-BD,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000006169,,S. cerevisiae,S. cerevisiae,Yeast-2-hybrid prey fusion Gal4 DNA-Binding-Domain,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,TCCAtg,ggAATG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTTCCATGAAGCTACTGTCATCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCACCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCGCGAGAGGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGGGAATGTGAGACCAGTATCTCAGCAAGGGCAACCGCTGATGTACTATTTGCCGTTAGGGATAACAGGGTAATAGTTGAAACCGTCCCTCGGTG,2565,AACGATGATGCTCACTCTCGGGTCTCTTCCATGAAGCTACTGTCATCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCACCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCGCGAGAGGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGGGAATGTGAGACCAGTATCTCAGCAAGGGCAACCGCTGATGTACTATTTGCCGTTAGGGATAACAGGGTAATAGTTGAAACCGTCCCTCGGTG,564,ColE1 High Copy,500-702,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000329.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/2547163,,we are unaware,freegenes.github.io/genes/images/BBF10K_000329.png,,,,,d71d20f0,False
2171,BBF10K_000335,N1N2_SV40_NLS,N1N2 SV40 Nuclear Localization Signal (NLS),Open Yeast Collection,pOpen-N1N2_SV40_NLS,pBuild,M13 For,M13 Rev,,,,,https://www.ncbi.nlm.nih.gov/nuccore/9628421,,SV40,yeast,"SV40 Nuclear Localization Signal (NLS) for Yeast-2-Hybrid or other fusions, targets the protein to the yeast nucleus.","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,CCATg,ggGTCA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGATATCTGGGGATCCCGGGAAAAGACCTATATTTGCGGTCCAACTTAGGCATAAACCTCGATGCTACCTACTCAGACCCACTCTGCACGAAGTAAATATGGCATTCATCCCAGCTGGTTCTTGGCGTTCTACGCAGGGTCTCTCCATGATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTGGGTCACGAGACCCCATATCGTCCTAATTCTTTGGTTATGTTTCCGATGTAGGAGTGAACCTACTTCCGTTTGCGTCTTATTACCAATGAAAAAGCTATGCACTTTGTATAGGGGATATCAGTATTAGGCGTCAAGGTCCAAGGTCTAAGCAGCACTTTTATAGGGATAACAGGGTAATGTACACTTTTCCGAAATTAAT,2411,AACGATGATGCTCACTCTCGGATATCTGGGGATCCCGGGAAAAGACCTATATTTGCGGTCCAACTTAGGCATAAACCTCGATGCTACCTACTCAGACCCACTCTGCACGAAGTAAATATGGCATTCATCCCAGCTGGTTCTTGGCGTTCTACGCAGGGTCTCTCCATGATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTGGGTCACGAGACCCCATATCGTCCTAATTCTTTGGTTATGTTTCCGATGTAGGAGTGAACCTACTTCCGTTTGCGTCTTATTACCAATGAAAAAGCTATGCACTTTGTATAGGGGATATCAGTATTAGGCGTCAAGGTCCAAGGTCTAAGCAGCACTTTTATAGGGATAACAGGGTAATGTACACTTTTCCGAAATTAAT,410,ColE1 High Copy,500-703,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000335.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/3023047/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000335.png,,,,,6e587fbb,False
2172,BBF10K_000355,Pp_aMF_sec_tag,Pichia aMF Secretion Tag,Open Yeast Collection,pOpen-Pp_aMF_sec_tag,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),Pichia aMF Secretion tag,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,TACT,tCCAT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGATATCGGTCTCTTACTATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTTTAGAGAAAAGAGAGGCTGAGGCTTCCATTGAGACCCTGATAGTGATATCAGTATTCTTACAGCCAGCCGGAGAGGCAAGCGGAAGAAAAATAGGGATAACAGGGTAATCACTCGCAAGGGCGGGTTTAG,2411,AACGATGATGCTCACTCTCGGATATCGGTCTCTTACTATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTTTAGAGAAAAGAGAGGCTGAGGCTTCCATTGAGACCCTGATAGTGATATCAGTATTCTTACAGCCAGCCGGAGAGGCAAGCGGAAGAAAAATAGGGATAACAGGGTAATCACTCGCAAGGGCGGGTTTAG,410,ColE1 High Copy,500-704,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000355.gb,Scott Pownall & Isaac Larkin,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000355.png,,,,,dffa59ae,False
2173,BBF10K_000357,Pp_aMF_noEAEA_sec_tag,Pichia aMF_noEAEA secretion tag,Open Yeast Collection,pOpen-Pp_aMF_noEAEA_sec_tag,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),Pichia aMF_noEAEA secretion tag,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,cds,TACT,tCCAT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGATATCGGTCTCTTACTATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTTTAGAGAAAAGATCCATTGAGACCCTGAGAGGCTGAGGCTTAGTGATATCAGTATTGCCGGACTAAACCCCACCCTCCTAGTGCCGAAAAGTAGGGATAACAGGGTAATGTCAAGCGTACGTCCGCTTAT,2411,AACGATGATGCTCACTCTCGGATATCGGTCTCTTACTATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTTTAGAGAAAAGATCCATTGAGACCCTGAGAGGCTGAGGCTTAGTGATATCAGTATTGCCGGACTAAACCCCACCCTCCTAGTGCCGAAAAGTAGGGATAACAGGGTAATGTCAAGCGTACGTCCGCTTAT,410,ColE1 High Copy,500-705,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000357.gb,Scott Pownall & Isaac Larkin,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000357.png,,,,,4e7b78b6,False
2175,BBF10K_000385,Sc-tUBX6,S. cerevisiae terminator UBX6,Open Yeast Collection,pOpen-ENO1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003486,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator ENO1,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTATGTGCTTTTGTTTATTTCCTATTTGGTTATTCACACATATTTTCTTTCTTCCGTCTATTAAAATATTCATATTTATTTTTTATTTATACATATATATTTATTTATTAGTCACTCAGTTCGTCAACGTGGTTGTTCTTCCATCATGATTTTTCTTATCTCATTATCGTGGTTTTCATTATCTTCGCTTGAGACCAGTCCGACACAATGTGACACCGTCGATGTCATGCTTCCCACTAGGGATAACAGGGTAATCCTCGGGCTCTGCCCGAATATGAATTC,2312,AACGATGATGCTCACTCTCGGGTCTCTGCTTATGTGCTTTTGTTTATTTCCTATTTGGTTATTCACACATATTTTCTTTCTTCCGTCTATTAAAATATTCATATTTATTTTTTATTTATACATATATATTTATTTATTAGTCACTCAGTTCGTCAACGTGGTTGTTCTTCCATCATGATTTTTCTTATCTCATTATCGTGGTTTTCATTATCTTCGCTTGAGACCAGTCCGACACAATGTGACACCGTCGATGTCATGCTTCCCACTAGGGATAACAGGGTAATCCTCGGGCTCTGCCCGAATATGAATTC,311,ColE1 High Copy,500-705,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000385.gb,,,,,freegenes.github.io/genes/images/BBF10K_000385.png,,,,,89c5d619,False
2176,BBF10K_000392,Sc-tENO1,S. cerevisiae terminator ENO1,Open Yeast Collection,pOpen-ENO1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003486,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator ENO1,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTAGCTTTTGATTAAGCCTTCTAGTCCAAAAAACACGTTTTTTTGTCATTTATTTCATTTTCTTAGAATAGTTTAGTTTATTCATTTTATAGTCACGAATGTTTTATGATTCTATATAGGGTTGCAAACAAGCATTTTTCATTTTATGTTAAAACAATTTCAGGTTTACCTTTTATTCTGCTTGTGGTGACGCGTGTATCCGCCCGCTCTTTTGGTCACCCATGTATCGCTTGAGACCTCATCAAGGCAATTAAGTATTTCGCTTTCAGCAGACTCTAGTCGTAAAGTTTCTATGGATATCAGTGAACTGACCGAATCCTCCGAGTGGGGACCTTATCCTAGTAGGGATAACAGGGTAATGACAACGTTCTTCTCTTTAAT,2411,AACGATGATGCTCACTCTCGGGTCTCTGCTTAGCTTTTGATTAAGCCTTCTAGTCCAAAAAACACGTTTTTTTGTCATTTATTTCATTTTCTTAGAATAGTTTAGTTTATTCATTTTATAGTCACGAATGTTTTATGATTCTATATAGGGTTGCAAACAAGCATTTTTCATTTTATGTTAAAACAATTTCAGGTTTACCTTTTATTCTGCTTGTGGTGACGCGTGTATCCGCCCGCTCTTTTGGTCACCCATGTATCGCTTGAGACCTCATCAAGGCAATTAAGTATTTCGCTTTCAGCAGACTCTAGTCGTAAAGTTTCTATGGATATCAGTGAACTGACCGAATCCTCCGAGTGGGGACCTTATCCTAGTAGGGATAACAGGGTAATGACAACGTTCTTCTCTTTAAT,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000392.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000392.png,,,,,47b01b59,False
2177,BBF10K_000401,Sc-tENO2,S. cerevisiae terminator ENO2,Open Yeast Collection,pOpen-ENO2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000001217,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator ENO2,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATCGCTTGAGACCACGTAATCTTAAGCAGTGCTTATCGGAAAATCTATTAGCAAAGAACCATTCAGCGACGATATCAGTGGTCTGTAAACCGTACCCATGACCGCACGACTGAGATATAGGGATAACAGGGTAATAATATGTCGTGTGCAGGCAGC,2411,AACGATGATGCTCACTCTCGGGTCTCTGCTTAGTGCTTTTAACTAAGAATTATTAGTCTTTTCTGCTTATTTTTTCATCATAGTTTAGAACACTTTATATTAACGAATAGTTTATGAATCTATTTAGGTTTAAAAATTGATACAGTTTTATAAGTTACTTTTTCAAAGACTCGTGCTGTCTATTGCATAATGCACTGGAAGGGGAAAAAAAAGGTGCACACGCGTGGCTTTTTCTTGAATTTGCAGTTTGAAAAATCGCTTGAGACCACGTAATCTTAAGCAGTGCTTATCGGAAAATCTATTAGCAAAGAACCATTCAGCGACGATATCAGTGGTCTGTAAACCGTACCCATGACCGCACGACTGAGATATAGGGATAACAGGGTAATAATATGTCGTGTGCAGGCAGC,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000401.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000401.png,,,,,7beb929a,False
2178,BBF10K_000406,Sc-tPRM9,S. cerevisiae terminator PRM9,Open Yeast Collection,pOpen-PRM9,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000078,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator PRM9,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATCGCTTGAGACCAGTGTTTTCCATTTTCCGCGAAACAACTAGAAGCTGATGCGTAGGGATAACAGGGTAATATGTGCTAGGCGAAAACTTGA,2373,AACGATGATGCTCACTCTCGGGTCTCTGCTTACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATCGCTTGAGACCAGTGTTTTCCATTTTCCGCGAAACAACTAGAAGCTGATGCGTAGGGATAACAGGGTAATATGTGCTAGGCGAAAACTTGA,372,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000406.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000406.png,,,,,3e0b0d96,False
2179,BBF10K_000412,Sc-tSSA1,S. cerevisiae terminator SSA1,Open Yeast Collection,pOpen-SSA1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000004,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator SSA1,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTGCCAATTGGTGCGGCAATTGATAATAACGAAAATGTCTTTTAATGATCTGGGTATAATGAGGAATTTTCCGAACGTTTTTACTTTATATATATATATACATGTAACATATATTCTATACGCTATAGAGAAAGGAAATTTTTCAATTAAAAAAAAATAGAGAAAGAGTTTCACTTCTTGATTATCGCTAACACTAATGGTTGAAGTACTGCTACTTTAATTTTATCGCTTGAGACCACTCGAGAGTTATAAGGGTCCGATGCCTTGGTTTTCATCTTCGGCCAATAGGGATAACAGGGTAATTGCGTCCTTATGCGCTTCTGC,2354,AACGATGATGCTCACTCTCGGGTCTCTGCTTGCCAATTGGTGCGGCAATTGATAATAACGAAAATGTCTTTTAATGATCTGGGTATAATGAGGAATTTTCCGAACGTTTTTACTTTATATATATATATACATGTAACATATATTCTATACGCTATAGAGAAAGGAAATTTTTCAATTAAAAAAAAATAGAGAAAGAGTTTCACTTCTTGATTATCGCTAACACTAATGGTTGAAGTACTGCTACTTTAATTTTATCGCTTGAGACCACTCGAGAGTTATAAGGGTCCGATGCCTTGGTTTTCATCTTCGGCCAATAGGGATAACAGGGTAATTGCGTCCTTATGCGCTTCTGC,353,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000412.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000412.png,,,,,2e5c5151,False
2180,BBF10K_000416,Sc-tTDH1,S. cerevisiae terminator TDH1,Open Yeast Collection,pOpen-TDH1,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000003588,,S. cerevisiae,S. cerevisiae,S. cerevisiae terminator TDH1,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTGCTTATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGACGCTTGAGACCAATTAAGTATTTCGCTTTCAGCAGACTCTAGTATTCAGCGACTAGACATGGGAACTAGATATCAGTTGCAGTATCTAACCCGCGGTTTTGGTTGGCCTTGCGCATAGGGATAACAGGGTAATTAGTTAAATATGGACATTCCG,2411,AACGATGATGCTCACTCTCGGGTCTCTGCTTATAAAGCAATCTTGATGAGGATAATGATTTTTTTTTGAATATACATAAATACTACCGTTTTTCTGCTAGATTTTGTGATGACGTAAATAAGTACATATTACTTTTTAAGCCAAGACAAGATTAAGCATTAACTTTACCCTTTTCTTTCTAAGTTTCAATATTAGTTATCACTGTTTAAAAGTTATGGCGAGAACGTCGGCGGTTAAAATATATTACCCTGAACGACGCTTGAGACCAATTAAGTATTTCGCTTTCAGCAGACTCTAGTATTCAGCGACTAGACATGGGAACTAGATATCAGTTGCAGTATCTAACCCGCGGTTTTGGTTGGCCTTGCGCATAGGGATAACAGGGTAATTAGTTAAATATGGACATTCCG,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000416.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000416.png,,,,,f4e27c75,False
2181,BBF10K_000418,Pp-tAOX1,Pichia terminator AOX1,Open Yeast Collection,pOpen-TDH2,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),Pichia (Komagataella pastoris) terminator,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,terminator,GCTT,CGCT,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGATATCGGTCTCTGCTTTCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGACGCTTGAGACCCGTAATGATCAGAGTGTTCATAGCATTCAGATATCAGTTGTAATATCACCCGCGCTGGGCAATAGGAAACTGTGAGTAGGGATAACAGGGTAATATAATTTCGTCACGAATCGGT,2411,AACGATGATGCTCACTCTCGGATATCGGTCTCTGCTTTCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGACGCTTGAGACCCGTAATGATCAGAGTGTTCATAGCATTCAGATATCAGTTGTAATATCACCCGCGCTGGGCAATAGGAAACTGTGAGTAGGGATAACAGGGTAATATAATTTCGTCACGAATCGGT,410,ColE1 High Copy,500-701,BSL2,,,https://freegenes.github.io/genbank/BBF10K_000418.gb,Scott Pownall & Isaac Larkin,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000418.png,,,,,2d051309,False
2182,BBF10K_000420,AConL-start-rev,assembly connector left start-rev,Open Yeast Collection,pOpen-AConL-start-rev,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCAATGAGAATTCGCATCTAGAGGAGGTGTCTTCGCCTAGCACAGGTCAGTGCTTTGACGGGAGTCGTTAGTTGCACCCATAGGGCCAGAGCACTGCGCGTATTACACTACATCCATGACTTATAATGAGTGGCCCTCAGCGCACGGCGTGGCACCGCATACAAGACGAATACCTCTGGAGTGAGACCAGAAGCTTCCATACGTGGCTAGCATGAGACAAAAAGTCATTGATCACTGTGTAGGGATAACAGGGTAATCGTCGCACTTGGAGAGAACCC,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCAATGAGAATTCGCATCTAGAGGAGGTGTCTTCGCCTAGCACAGGTCAGTGCTTTGACGGGAGTCGTTAGTTGCACCCATAGGGCCAGAGCACTGCGCGTATTACACTACATCCATGACTTATAATGAGTGGCCCTCAGCGCACGGCGTGGCACCGCATACAAGACGAATACCTCTGGAGTGAGACCAGAAGCTTCCATACGTGGCTAGCATGAGACAAAAAGTCATTGATCACTGTGTAGGGATAACAGGGTAATCGTCGCACTTGGAGAGAACCC,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000420.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871411,,we are unaware,freegenes.github.io/genes/images/BBF10K_000420.png,,,,,cf62172f,False
2183,BBF10K_000424,AConL-start,assembly connector left start,Open Yeast Collection,pOpen-AConL-start,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAGCCTAGCACAGGTCAGTGCTTTGACGGGAGTCGTTAGTTGCACCCATAGGGCCAGAGCACTGCGCGTATTACACTACATCCATGACTTATAATGAGTGGCCCTCAGCGCACGGCGTGGCACCGCATACAAGACGAATACCTCTGAAGACACGGAGACTGGCCGATAATTGCAGACGGGAGTGAGACCAGATACTGTAAGAACCACGCGGGAGCGTTCGCCAGAAGTGCGATAGGGATAACAGGGTAATCTCCGGGAATATAAACGGTGA,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAGCCTAGCACAGGTCAGTGCTTTGACGGGAGTCGTTAGTTGCACCCATAGGGCCAGAGCACTGCGCGTATTACACTACATCCATGACTTATAATGAGTGGCCCTCAGCGCACGGCGTGGCACCGCATACAAGACGAATACCTCTGAAGACACGGAGACTGGCCGATAATTGCAGACGGGAGTGAGACCAGATACTGTAAGAACCACGCGGGAGCGTTCGCCAGAAGTGCGATAGGGATAACAGGGTAATCTCCGGGAATATAAACGGTGA,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000424.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871405,,we are unaware,freegenes.github.io/genes/images/BBF10K_000424.png,,,,,597457b2,False
2184,BBF10K_000429,AConL1,assembly connector left 1,Open Yeast Collection,pOpen-AConL1,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGACTATACACGGGCCGTGACCGTGAAGTAGCTCTATGACCTGGATGAAGAATAGACCCCCGTGAGCCGACTTGTGCAGGCCACTATATGTTCGAGCACCTCGGAGAGAGGGCACTAGCTCACAAGTTGCTACACAGCATCTTCGGGAAGACACTGACACCAGATGTCAACACAGCTACGGAGTGAGACCAGATAGATCATGTCGGCAGTCGGCGCAATCGCGCGCACGGTTCTAGGGATAACAGGGTAATATTGGTGCATATGGGCGTACT,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGACTATACACGGGCCGTGACCGTGAAGTAGCTCTATGACCTGGATGAAGAATAGACCCCCGTGAGCCGACTTGTGCAGGCCACTATATGTTCGAGCACCTCGGAGAGAGGGCACTAGCTCACAAGTTGCTACACAGCATCTTCGGGAAGACACTGACACCAGATGTCAACACAGCTACGGAGTGAGACCAGATAGATCATGTCGGCAGTCGGCGCAATCGCGCGCACGGTTCTAGGGATAACAGGGTAATATTGGTGCATATGGGCGTACT,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000429.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871406,,we are unaware,freegenes.github.io/genes/images/BBF10K_000429.png,,,,,a76ba533,False
2185,BBF10K_000432,AConL2,assembly connector left 2,Open Yeast Collection,pOpen-AConL2,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGACTAAAAAGTCTCTATCGCCGGTTCTTGAGTTATTATGGATAGGCTGATTAGCGTGGGACTTCTCTAAAGTGGCGCGTCCACTCTTCGGCAGGACTCTTCGCCCGCCCCAAGTAGTCACTCTGTGTGTCAAAGTGACCTTGGCAGAAGACACTCCCCACACACTGGCTTAAGATGACGGAGTGAGACCAGATAGATGGTGCCTACATGCGACGACAGAATATGTTATATAATAGGGATAACAGGGTAATTACAACACCCAAGTGCACTGT,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGACTAAAAAGTCTCTATCGCCGGTTCTTGAGTTATTATGGATAGGCTGATTAGCGTGGGACTTCTCTAAAGTGGCGCGTCCACTCTTCGGCAGGACTCTTCGCCCGCCCCAAGTAGTCACTCTGTGTGTCAAAGTGACCTTGGCAGAAGACACTCCCCACACACTGGCTTAAGATGACGGAGTGAGACCAGATAGATGGTGCCTACATGCGACGACAGAATATGTTATATAATAGGGATAACAGGGTAATTACAACACCCAAGTGCACTGT,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000432.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871407,,we are unaware,freegenes.github.io/genes/images/BBF10K_000432.png,,,,,0c1e2db5,False
2186,BBF10K_000439,AConL3,assembly connector left 3,Open Yeast Collection,pOpen-AConL3,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGATCCTGAAAAGATGAGCATTAGCGTCATCCACGGATCAGCGATGACGTGGACACCCCGCTGGTGCCCTATGGTGTCCGCAGGAACATATCACTTCGCGTTCGCCTAGTATACATGCGAACGCGGAGACAGAAATCGTCAGTCATGAAGACAGTACTTGTATGGGCACAGACAACCTAGGAGTGAGACCAGATCACAACAAAGGACGGGTCCCCTCTGTAATGGACAGAAGGTAGGGATAACAGGGTAATGATAAAGCCGACTCACACAAG,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGATCCTGAAAAGATGAGCATTAGCGTCATCCACGGATCAGCGATGACGTGGACACCCCGCTGGTGCCCTATGGTGTCCGCAGGAACATATCACTTCGCGTTCGCCTAGTATACATGCGAACGCGGAGACAGAAATCGTCAGTCATGAAGACAGTACTTGTATGGGCACAGACAACCTAGGAGTGAGACCAGATCACAACAAAGGACGGGTCCCCTCTGTAATGGACAGAAGGTAGGGATAACAGGGTAATGATAAAGCCGACTCACACAAG,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000439.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871408,,we are unaware,freegenes.github.io/genes/images/BBF10K_000439.png,,,,,2694d30b,False
2187,BBF10K_000440,AConL4,assembly connector left 4,Open Yeast Collection,pOpen-AConL4,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAATCATCCGCAGTAGTTCTTTAGAGGCGGCGATTGAGATATATCTTCCAGAGCGACGTCGGCCAAGAAACTCGTTCTTCACGGAACCCGAGTAACGAACCTATCGTAGCAAGGACGGCACTAGCCACACGGCTGGAGCGACGACGAAGACACCCATGACTACCCATGAGTCACAATGGGAGTGAGACCAGATCAGACAACACAGAGGCTGGCCTACAAGATCCCATATGTCTAGGGATAACAGGGTAATACCTCAATAGCTCCGAAAACC,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAATCATCCGCAGTAGTTCTTTAGAGGCGGCGATTGAGATATATCTTCCAGAGCGACGTCGGCCAAGAAACTCGTTCTTCACGGAACCCGAGTAACGAACCTATCGTAGCAAGGACGGCACTAGCCACACGGCTGGAGCGACGACGAAGACACCCATGACTACCCATGAGTCACAATGGGAGTGAGACCAGATCAGACAACACAGAGGCTGGCCTACAAGATCCCATATGTCTAGGGATAACAGGGTAATACCTCAATAGCTCCGAAAACC,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000440.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871409,,we are unaware,freegenes.github.io/genes/images/BBF10K_000440.png,,,,,9ebbba2a,False
2188,BBF10K_000441,AConL5,assembly connector left 5,Open Yeast Collection,pOpen-AConL5,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,left-connector,ATGA,GGAG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAATGCTCGGGCACATGCGGTGAATTGAGATAACGCTTGGCGCTTCGGTGGATTCTTCGCTCAACCAACCAGTGATGCTGTGTTAGATGCATCCATATTCAGCCCTAGTAGTGCCAGAAACATACGTGACCTCACATTGCGCTCCGAAGACACAATGGAATAAAGCTCCACACAGTCGGGAGTGAGACCAGATCCAGGAGGTCTAGGAACCTTGAAAGATGGTTTAGCTACCTAGGGATAACAGGGTAATATACTGGTATTGTGGGTGAAC,2318,AACGATGATGCTCACTCTCGTCGGTCTCAATGAGAATTCGCATCTAGAATGCTCGGGCACATGCGGTGAATTGAGATAACGCTTGGCGCTTCGGTGGATTCTTCGCTCAACCAACCAGTGATGCTGTGTTAGATGCATCCATATTCAGCCCTAGTAGTGCCAGAAACATACGTGACCTCACATTGCGCTCCGAAGACACAATGGAATAAAGCTCCACACAGTCGGGAGTGAGACCAGATCCAGGAGGTCTAGGAACCTTGAAAGATGGTTTAGCTACCTAGGGATAACAGGGTAATATACTGGTATTGTGGGTGAAC,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000441.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871410,,we are unaware,freegenes.github.io/genes/images/BBF10K_000441.png,,,,,07de1653,False
2189,BBF10K_000442,AConR1,assembly connector right 1,Open Yeast Collection,pOpen-AConR1,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTGACGTGTCTTCCAAGACTGTAGCTTCCGCCGCCGACTAACGTTCGACGTACCATGTTAATTACATGGGGTTCTCTACCTCCATCTCGCGTGAATATTGGCTGTTCGGCCAGTACGGAAGGTTGCATGGGATAGATGTTCATCTAGTGACCATCCACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATCCTAGAAAAGGCGAAGGCGCTAACCGCTGGGGCTTAAATTAGGGATAACAGGGTAATCAAGGCGTTTTCCGCGGGGAG,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTGACGTGTCTTCCAAGACTGTAGCTTCCGCCGCCGACTAACGTTCGACGTACCATGTTAATTACATGGGGTTCTCTACCTCCATCTCGCGTGAATATTGGCTGTTCGGCCAGTACGGAAGGTTGCATGGGATAGATGTTCATCTAGTGACCATCCACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATCCTAGAAAAGGCGAAGGCGCTAACCGCTGGGGCTTAAATTAGGGATAACAGGGTAATCAAGGCGTTTTCCGCGGGGAG,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000442.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871412,,we are unaware,freegenes.github.io/genes/images/BBF10K_000442.png,,,,,258671fd,False
2190,BBF10K_000443,AConR2,assembly connector right 2,Open Yeast Collection,pOpen-AConR2,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTCCCGTGTCTTCCGCGTTTACAATCACGAGACTGTTTCTAGGATGACAGGTACGCACGGCGTCACTATAGTACGCTCACTTCTCGGCTCGATAAGTATAGTGTAACCTACATTAACGCATCTGCCCAGGAGTAATGGTAACGCACCCCATCCCCGACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATGCCATGACGACAACTAGCGCTCACAACTAGAGGGTGCCATAGGGATAACAGGGTAATCGTACTAACGCAATGTGACTC,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTCCCGTGTCTTCCGCGTTTACAATCACGAGACTGTTTCTAGGATGACAGGTACGCACGGCGTCACTATAGTACGCTCACTTCTCGGCTCGATAAGTATAGTGTAACCTACATTAACGCATCTGCCCAGGAGTAATGGTAACGCACCCCATCCCCGACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATGCCATGACGACAACTAGCGCTCACAACTAGAGGGTGCCATAGGGATAACAGGGTAATCGTACTAACGCAATGTGACTC,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000443.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871413,,we are unaware,freegenes.github.io/genes/images/BBF10K_000443.png,,,,,dccce758,False
2191,BBF10K_000444,AConR3,assembly connector right 3,Open Yeast Collection,pOpen-AConR3,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTACTGTGTCTTCTGAAGTTCGCATGGTCCTCCAGCATGTTCTTCCGAAAGTCAGACAATAGGTTCACAAGCATGTTCCGTGACGGGCAGTAACTATCACTCCGAAAGTAGGTTCTAAGTCGGCGTGAGTGGATGACGGAAAGTTTGTCCTTAGGTACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATGCTAGCTGGAACTATCGGGACACCAGCGCGTTGTGACTATAGGGATAACAGGGTAATCTGCGCCGATTGTTTGTGCGT,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTTACTGTGTCTTCTGAAGTTCGCATGGTCCTCCAGCATGTTCTTCCGAAAGTCAGACAATAGGTTCACAAGCATGTTCCGTGACGGGCAGTAACTATCACTCCGAAAGTAGGTTCTAAGTCGGCGTGAGTGGATGACGGAAAGTTTGTCCTTAGGTACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATGCTAGCTGGAACTATCGGGACACCAGCGCGTTGTGACTATAGGGATAACAGGGTAATCTGCGCCGATTGTTTGTGCGT,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000444.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871414,,we are unaware,freegenes.github.io/genes/images/BBF10K_000444.png,,,,,cde9effa,False
2192,BBF10K_000445,AConR4,assembly connector right 4,Open Yeast Collection,pOpen-AConR4,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTCCATGTGTCTTCGGATGTGCGTGCGAAAAGTTGCGTTACAGTTCATCAAACGCTATACTGCCATGTGGACCTTGGACGACGGCCGTAGTTGCCAGCTCTCGCATACTGCGGTTCAACTTATTACTCCTAACTCGTGTTAACGTTACTTTTCTAAGACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTAGGATTGCGAGCGACACGTATACTGCCGACAAGCGTGGTAGGGATAACAGGGTAATAAACCCGGGTTCAGCCGGTGT,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTCCATGTGTCTTCGGATGTGCGTGCGAAAAGTTGCGTTACAGTTCATCAAACGCTATACTGCCATGTGGACCTTGGACGACGGCCGTAGTTGCCAGCTCTCGCATACTGCGGTTCAACTTATTACTCCTAACTCGTGTTAACGTTACTTTTCTAAGACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTAGGATTGCGAGCGACACGTATACTGCCGACAAGCGTGGTAGGGATAACAGGGTAATAAACCCGGGTTCAGCCGGTGT,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000445.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871415,,we are unaware,freegenes.github.io/genes/images/BBF10K_000445.png,,,,,7bb613a1,False
2193,BBF10K_000446,AConR5,assembly connector right 5,Open Yeast Collection,pOpen-AConR5,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTAAGTGTGTCTTCCCTGATAATATAGAAGTGACTTGCCACCACTGCCACGCCGAACAGAGGCGAAAAAACACGCGGCTAGGCGACCTGTAGACCTCATGATACATAGCGATCCTTGTTCGATGGGAGCAAAAGAACACTAAGCGATACCCCGAAGAACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTAGTACACTCCGTGAGCGCCAAGTTGTTACGGGAGCGAGTAGGGATAACAGGGTAATACTGTATGGCAGACTTCATGC,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTAAGTGTGTCTTCCCTGATAATATAGAAGTGACTTGCCACCACTGCCACGCCGAACAGAGGCGAAAAAACACGCGGCTAGGCGACCTGTAGACCTCATGATACATAGCGATCCTTGTTCGATGGGAGCAAAAGAACACTAAGCGATACCCCGAAGAACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTAGTACACTCCGTGAGCGCCAAGTTGTTACGGGAGCGAGTAGGGATAACAGGGTAATACTGTATGGCAGACTTCATGC,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000446.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871416,,we are unaware,freegenes.github.io/genes/images/BBF10K_000446.png,,,,,d27e0ca2,False
2194,BBF10K_000447,AConR-end,assembly connector right end,Open Yeast Collection,pOpen-AConR-end,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTAGCCGTGTCTTCGCGGACCCAACATCCTCCTAGTGAGAAAGGAGCATATTTTGCTTTGGATCGGTGAAACATATAGACATGCGTACAACTGACTCTCTGTGAACGACTGGCCGTCTTGCAGAACTGACCTACCCACGCTGGTGTGACTGACTTGCACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTCAAGGGTTGGACGACTCCCTAAGCACTCTAGTGGAACATAGGGATAACAGGGTAATGCCGGACTGCTTTCTGCAACC,2311,AACGATGATGCTCACTCTCGCTCGAGTCGGTCTCACGCTAGCCGTGTCTTCGCGGACCCAACATCCTCCTAGTGAGAAAGGAGCATATTTTGCTTTGGATCGGTGAAACATATAGACATGCGTACAACTGACTCTCTGTGAACGACTGGCCGTCTTGCAGAACTGACCTACCCACGCTGGTGTGACTGACTTGCACTAGTGCACTGCAGAGACTGAGACCAGAAGCTTCCATTCAAGGGTTGGACGACTCCCTAAGCACTCTAGTGGAACATAGGGATAACAGGGTAATGCCGGACTGCTTTCTGCAACC,310,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000447.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871417,,we are unaware,freegenes.github.io/genes/images/BBF10K_000447.png,,,,,aa11c198,False
2195,BBF10K_000448,AConR-end-rev,assembly connector right end-rev,Open Yeast Collection,pOpen-AConR-end-rev,pBuild,M13 For,M13 Rev,,,,,,,synthetic,Species agnostic,"For multi-gene assembly, uses BbsI sites for level 2 assembly, fully custom sequence, based on Dueber's design","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI/BbsI,right-connector,CGCT,AGAC,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGTCGGTCTCACGCTGCGGACCCAACATCCTCCTAGTGAGAAAGGAGCATATTTTGCTTTGGATCGGTGAAACATATAGACATGCGTACAACTGACTCTCTGTGAACGACTGGCCGTCTTGCAGAACTGACCTACCCACGCTGGTGTGACTGACTTGCGAAGACACAGCCGTTACAGAGATGTTACGAACCACTAGTGCACTGCAGAGACTGAGACCAGATTCTCACGACGCAAGATGGTCCAGGCACTTCTGGCAGATGTAGGGATAACAGGGTAATGCTCACGTTGGCACCAAGCCG,2318,AACGATGATGCTCACTCTCGTCGGTCTCACGCTGCGGACCCAACATCCTCCTAGTGAGAAAGGAGCATATTTTGCTTTGGATCGGTGAAACATATAGACATGCGTACAACTGACTCTCTGTGAACGACTGGCCGTCTTGCAGAACTGACCTACCCACGCTGGTGTGACTGACTTGCGAAGACACAGCCGTTACAGAGATGTTACGAACCACTAGTGCACTGCAGAGACTGAGACCAGATTCTCACGACGCAAGATGGTCCAGGCACTTCTGGCAGATGTAGGGATAACAGGGTAATGCTCACGTTGGCACCAAGCCG,317,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000448.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871418,,we are unaware,freegenes.github.io/genes/images/BBF10K_000448.png,,,,,12512be6,False
2196,BBF10K_000449,ScHR5'-LEU2,S. cerevisiae target 5'-Homology Region LEU2,Open Yeast Collection,pOpen-ScHR5'-LEU2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000523,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae LEU2 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,CAGG,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCACCCAGAAGAACAGTAAAATAAAGCAAGGTACGTGAAATTAATATTTTTAAATGGTTCTAACCGATGCCGAAGAACTGCGCAGTCCGGTTATAACGTCTGACATGTCCTTTTTTGATTTGGAATCCAACCACTCAAGTGACTCTGTTCATTTACTTTGCGAAAAATATACCCACAAATTGCCCATCGAAAGTGAATCGCAAACCACCTTCAGACTGGCACCGACAAAGCAAAGATTATACAGACAGAGTACTTTATACGTACCGTTAAGTCTCAAGCAAAGGGTTTTCTTATTTACTGAACGGGTAAAGAGTATCTGGGCCGGCTTGCCAAGATGCAAACCGAATAAGTATTTCAAAGTTGCATTTGCCTTAGCCGTCCTGACACCATTGGCTATTTGGATATTTTATATTGACTTTCGTGTACATTGATCACATCGACTGTTCTATTGGCAAATGAACCACGGGCATTGACTATTTTTCAGGTTACTACTATATATTATCATCACGGGCAAGGATTGTAAAGGTGAGACCATTGACGGGAACTACACTCGATGGTTTTTCCGCTTGTGGTATAGGGATAACAGGGTAATTGCTGTAACCCGTTTTGACGC,2650,AACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCACCCAGAAGAACAGTAAAATAAAGCAAGGTACGTGAAATTAATATTTTTAAATGGTTCTAACCGATGCCGAAGAACTGCGCAGTCCGGTTATAACGTCTGACATGTCCTTTTTTGATTTGGAATCCAACCACTCAAGTGACTCTGTTCATTTACTTTGCGAAAAATATACCCACAAATTGCCCATCGAAAGTGAATCGCAAACCACCTTCAGACTGGCACCGACAAAGCAAAGATTATACAGACAGAGTACTTTATACGTACCGTTAAGTCTCAAGCAAAGGGTTTTCTTATTTACTGAACGGGTAAAGAGTATCTGGGCCGGCTTGCCAAGATGCAAACCGAATAAGTATTTCAAAGTTGCATTTGCCTTAGCCGTCCTGACACCATTGGCTATTTGGATATTTTATATTGACTTTCGTGTACATTGATCACATCGACTGTTCTATTGGCAAATGAACCACGGGCATTGACTATTTTTCAGGTTACTACTATATATTATCATCACGGGCAAGGATTGTAAAGGTGAGACCATTGACGGGAACTACACTCGATGGTTTTTCCGCTTGTGGTATAGGGATAACAGGGTAATTGCTGTAACCCGTTTTGACGC,649,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000449.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000449.png,,,,,3196c520,False
2197,BBF10K_000450,ScHR3'-LEU2,S. cerevisiae target LEU2 3'-Homology Region,Open Yeast Collection,pOpen-ScHR3'-LEU2,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000523,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae LEU2 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAGACACTCGTATCGCATGTCGGTGGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCAAGAAGGAGAAAAAGGAGGATGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTATGATTCCTAATTTATATATTGGAGGATTTTCTCTGCGGCCGCACGAATGAGACCCACTCGATAGGTACAACCGGTAACGGTTTGTCAGAGATCCCTAGGGATAACAGGGTAATAACCGCTGTGAGAGTATGCAT,2652,AACGATGATGCTCACTCTCGGGTCTCTAGACACTCGTATCGCATGTCGGTGGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAAAAAGATTCTCTTTTTTTATGATATTTGTACATAAACTTTATAAATGAAATTCATAATAGAAACGACACGAAATTACAAAATGGAATATGTTCATAGGGTAGACGAAACTATATACGCAATCTACATACATTTATCAAGAAGGAGAAAAAGGAGGATGTAAAGGAATACAGGTAAGCAAATTGATACTAATGGCTCAACGTGATAAGGAAAAAGAATTGCACTTTAACATTAATATTGACAAGGAGGAGGGCACCACACAAAAAGTTAGGTGTAACAGAAAATCATGAAACTATGATTCCTAATTTATATATTGGAGGATTTTCTCTGCGGCCGCACGAATGAGACCCACTCGATAGGTACAACCGGTAACGGTTTGTCAGAGATCCCTAGGGATAACAGGGTAATAACCGCTGTGAGAGTATGCAT,651,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000450.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000450.png,,,,,50a5a7ae,False
2198,BBF10K_000451,ScHR5'-URA3,S. cerevisiae target 5'-Homology Region URA3,Open Yeast Collection,pOpen-ScHR3'-URA3,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000747,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae URA3 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCGCTAAATTCGAGTGAAACACAGGAAGATCAGAAAATCCTCATTTCATCCATATTAACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGCAAGACAAGCAACGAAACGTTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACTCAGATATTGAAAAAAAGCTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCATGGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCAATTGATGACAATACAGACGATGATAACAAACCGAAGTTATCTGATGTAGAAAAGGATTAAAGATGCTAAGAGATAGTGATGATATTTCATAAATAATGTAATTCTATATATGTTAATTACCTTTTTTGCGAGGCATATTTATGGTGAAGGATAAGTTTTGACCATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATAAAGCCCACATGGATAACATTACAAGGTGAGACCCAGACCTACGGATCTTAGCGCTAGGGCCCTAGTATACTCACTAGGGATAACAGGGTAATCTGAGGTGAAAGAGTTGACGC,2650,AACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCGCTAAATTCGAGTGAAACACAGGAAGATCAGAAAATCCTCATTTCATCCATATTAACAATAATTTCAAATGTTTATTTGCATTATTTGAAACTAGGCAAGACAAGCAACGAAACGTTTTTGAAAATTTTGAGTATTTTCAATAAATTTGTAGAGGACTCAGATATTGAAAAAAAGCTACAGCAATTAATACTTGATAAGAAGAGTATTGAGAAGGGCAACGGTTCATCATCTCATGGATCTGCACATGAACAAACACCAGAGTCAAACGACGTTGAAATTGAGGCTACTGCGCCAATTGATGACAATACAGACGATGATAACAAACCGAAGTTATCTGATGTAGAAAAGGATTAAAGATGCTAAGAGATAGTGATGATATTTCATAAATAATGTAATTCTATATATGTTAATTACCTTTTTTGCGAGGCATATTTATGGTGAAGGATAAGTTTTGACCATCAAAGAAGGTTAATGTGGCTGTGGTTTCAGGGTCCATAAAGCCCACATGGATAACATTACAAGGTGAGACCCAGACCTACGGATCTTAGCGCTAGGGCCCTAGTATACTCACTAGGGATAACAGGGTAATCTGAGGTGAAAGAGTTGACGC,649,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000451.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000451.png,,,,,2c3e03fa,False
2199,BBF10K_000452,ScHR3'-URA3,S. cerevisiae target URA3 3'-Homology Region,Open Yeast Collection,pOpen-ScHR3'-URA3,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000747,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae URA3 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAGACAGAGCACTTGAATCCACTGCCAGCTTCATGGCCTTTATAAAAAGGAACTATCCAATACCTCGCCAGAACCAAGTAACAGTATTTTACGGGGCACAAATCAAGAACAATAAGACAGGACTGTAAAGATGGACGCATTGAACTCCAAAGAACAACAAGAGTTCCAAAAAGTAGTGGAACAAAAGCAAATGAAGGATTTCATGCGTTTGTACTCTAATCTGGTAGAAAGATGTTTCACAGACTGTGTCAATGACTTCACAACATCAAAGCTAACCAATAAGGAACAAACATGCATCATGAAGTGCTCAGAAAAGTTCTTGAAGCATAGCGAACGTGTAGGGCAGCGTTTCCAAGAACAAAACGCTGCCTTGGGACAAGGCTTGGGCCGATAAGGTGTACTGGCGTATATATATCTAATTATGTATCTCTGGTGTAGCCCATTTTTAGCATGTAAATATAAAGAGAAACCATATCTAATCTAACCAAATCCAAACAAAATTCAATAGTTACTATCGCTTTTTTCTGCGGCCGCACGAATGAGACCCCACGAGATAAGAGGATGGCAGATTAAGGTCGGCGTCATTCTAGGGATAACAGGGTAATCTCAGACTGGAGATGCAATCT,2653,AACGATGATGCTCACTCTCGGGTCTCTAGACAGAGCACTTGAATCCACTGCCAGCTTCATGGCCTTTATAAAAAGGAACTATCCAATACCTCGCCAGAACCAAGTAACAGTATTTTACGGGGCACAAATCAAGAACAATAAGACAGGACTGTAAAGATGGACGCATTGAACTCCAAAGAACAACAAGAGTTCCAAAAAGTAGTGGAACAAAAGCAAATGAAGGATTTCATGCGTTTGTACTCTAATCTGGTAGAAAGATGTTTCACAGACTGTGTCAATGACTTCACAACATCAAAGCTAACCAATAAGGAACAAACATGCATCATGAAGTGCTCAGAAAAGTTCTTGAAGCATAGCGAACGTGTAGGGCAGCGTTTCCAAGAACAAAACGCTGCCTTGGGACAAGGCTTGGGCCGATAAGGTGTACTGGCGTATATATATCTAATTATGTATCTCTGGTGTAGCCCATTTTTAGCATGTAAATATAAAGAGAAACCATATCTAATCTAACCAAATCCAAACAAAATTCAATAGTTACTATCGCTTTTTTCTGCGGCCGCACGAATGAGACCCCACGAGATAAGAGGATGGCAGATTAAGGTCGGCGTCATTCTAGGGATAACAGGGTAATCTCAGACTGGAGATGCAATCT,652,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000452.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000452.png,,,,,6e383489,False
2200,BBF10K_000453,ScHR5'-HO,S. cerevisiae target 5'-Homology Region HO,Open Yeast Collection,pOpen-ScHR5'-HO,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002386,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae HO locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCTCTAATTGTATCGAGATCACTTTTCGTGATCCGCTAATCAGCGACGGTCACATTAGGTTTGCCAAGTCAGGGTATGAACCATACGATCAGTTTTCGTGAACCTGGTACGTATATTGTGGCGTTTGTGTATATTTTCATTCTTTGACAACAATCAATACCAACCTCAAATAGGAAAAGTAATAAGTTTGGCGTTACACCCCAAAAGACGCCAAACGGATCGAACTTACTCAATAGCAATTAGCGAGACAAAACCTACGTTAAGACCTGTAACCGATTTATCAAAGCACTCTGCGGTTCTTTCTTGGGAATATTACCTGGACATTTTGTGCCCTCAAGAAACGAGGCTCTACGAGCCTGTTGGAGCCCCTCAGACATTAGCCGCCACGAATCAAACTTTTTACGCGATTCGGCCCAAATCAGTTTCTCACAGATCATTCGTAGAGTGAAAAAGCACATCGATTATTTGATACCCCTTTGGGTTAATTACTGTTGAGGTCTTTGCCGTAAGTTCCATCGCTGTAAGGTGAGACCCCAGAGCTTAGGGGACATACTTTCAAGTTCATTAGGGGTTATAGGGATAACAGGGTAATCACGGGTAGGGAGCACCCGGA,2650,AACGATGATGCTCACTCTCGGGTCTCTAAAACGGCCGCTCTAATTGTATCGAGATCACTTTTCGTGATCCGCTAATCAGCGACGGTCACATTAGGTTTGCCAAGTCAGGGTATGAACCATACGATCAGTTTTCGTGAACCTGGTACGTATATTGTGGCGTTTGTGTATATTTTCATTCTTTGACAACAATCAATACCAACCTCAAATAGGAAAAGTAATAAGTTTGGCGTTACACCCCAAAAGACGCCAAACGGATCGAACTTACTCAATAGCAATTAGCGAGACAAAACCTACGTTAAGACCTGTAACCGATTTATCAAAGCACTCTGCGGTTCTTTCTTGGGAATATTACCTGGACATTTTGTGCCCTCAAGAAACGAGGCTCTACGAGCCTGTTGGAGCCCCTCAGACATTAGCCGCCACGAATCAAACTTTTTACGCGATTCGGCCCAAATCAGTTTCTCACAGATCATTCGTAGAGTGAAAAAGCACATCGATTATTTGATACCCCTTTGGGTTAATTACTGTTGAGGTCTTTGCCGTAAGTTCCATCGCTGTAAGGTGAGACCCCAGAGCTTAGGGGACATACTTTCAAGTTCATTAGGGGTTATAGGGATAACAGGGTAATCACGGGTAGGGAGCACCCGGA,649,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000453.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000453.png,,,,,bf1566fb,False
2201,BBF10K_000454,ScHR3'-HO,S. cerevisiae target HO 3'-Homology Region,Open Yeast Collection,pOpen-ScHR3'-HO,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002386,,S. cerevisiae,S. cerevisiae,for stable genome integration adjacent to S. cerevisiae HO locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAGACTATCGTGTTGCATCTGCGGCTTTAAATTGATGTATCTCATCGCAGGCACGGGCAGTACAGTGCCCTGAGCGTAGGGAAAAATGAAAAAAAGGATGTAACTTTTAACATAATTCCAGCACGCAGCGATTGGGTATAATGAAGATTGTTAAGTTCAACAACATGTGAAATTCTTAGCTCCGCAACTTGCTTGAATTATGAGCTCTAAGATTCAAGAAGTAAATAGGTCATATAATGGAATGATAGTAGTACTTGATAAAGGAACCATGTGATCTTACGTTGATATGAAAATAATTCTCTCACAGAAGTCAGTTTGTATGACTAACATAGAGAGTAATTGCTTTTCGGATAAAATCGCACCTAACAAAGATTACCTTTTGTAAAGCCTCCAGAACAGCTATGAATGTTCGTTATTCTACGCACTTTCTTTCATACATCTTGATCCGCCTTTTTTGATGTAGGTTTTAACGCGGCAAAACATATTAATTTTTCTGATTTGGAAAAATAAAGAGTTAGTTCTGCCGCGGCCGCACGAATGAGACCCCCGAGGGGAGAAATATACCGGATTTGCGGGGGTGGGCATTTAGGGATAACAGGGTAATCCCGAAGAGTCCCAGCCGCTG,2652,AACGATGATGCTCACTCTCGGGTCTCTAGACTATCGTGTTGCATCTGCGGCTTTAAATTGATGTATCTCATCGCAGGCACGGGCAGTACAGTGCCCTGAGCGTAGGGAAAAATGAAAAAAAGGATGTAACTTTTAACATAATTCCAGCACGCAGCGATTGGGTATAATGAAGATTGTTAAGTTCAACAACATGTGAAATTCTTAGCTCCGCAACTTGCTTGAATTATGAGCTCTAAGATTCAAGAAGTAAATAGGTCATATAATGGAATGATAGTAGTACTTGATAAAGGAACCATGTGATCTTACGTTGATATGAAAATAATTCTCTCACAGAAGTCAGTTTGTATGACTAACATAGAGAGTAATTGCTTTTCGGATAAAATCGCACCTAACAAAGATTACCTTTTGTAAAGCCTCCAGAACAGCTATGAATGTTCGTTATTCTACGCACTTTCTTTCATACATCTTGATCCGCCTTTTTTGATGTAGGTTTTAACGCGGCAAAACATATTAATTTTTCTGATTTGGAAAAATAAAGAGTTAGTTCTGCCGCGGCCGCACGAATGAGACCCCCGAGGGGAGAAATATACCGGATTTGCGGGGGTGGGCATTTAGGGATAACAGGGTAATCCCGAAGAGTCCCAGCCGCTG,651,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000454.gb,Scott Pownall,https://pubmed.ncbi.nlm.nih.gov/25871405/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000454.png,,,,,391550d3,False
2202,BBF10K_000455,PpHR5'-NTS,Pichia NTS 3'-Homology Region,Open Yeast Collection,pOpen-PpHR3'-NTS,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia NTS locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAAAAAGCGGCCGCAAGACCAGCTTCGTAAGCAATCTGGACAATTATGTAAGCGGGTTACGTAAACAGTTATGTAAGCAGAAAAATTTCAAACGACAAAACTTGGGGTCTACAGACACAGTAGCCAGAAGATTGCACTACCATTCGACTCCTCATGACCCACTCTTTCGATCCATGTAGTTAGGTTACCGTTTTTCCTAATATTTAAGGATGTTGAAAATTCATTTTCATTTTTTTCGTTTTTAAGATTTTCTCACAACTCTTCCAAAGATTACTAGTGCCCACATGGATAACATTACAAGGTGAGACCCCGAGGGAACCATGATACAGGAAAACCAGGTTTTGCTAGTGTAGGGATAACAGGGTAATGCCCCTCTTGGGGCGTTACAC,2416,AACGATGATGCTCACTCTCGGGTCTCTAAAAAGCGGCCGCAAGACCAGCTTCGTAAGCAATCTGGACAATTATGTAAGCGGGTTACGTAAACAGTTATGTAAGCAGAAAAATTTCAAACGACAAAACTTGGGGTCTACAGACACAGTAGCCAGAAGATTGCACTACCATTCGACTCCTCATGACCCACTCTTTCGATCCATGTAGTTAGGTTACCGTTTTTCCTAATATTTAAGGATGTTGAAAATTCATTTTCATTTTTTTCGTTTTTAAGATTTTCTCACAACTCTTCCAAAGATTACTAGTGCCCACATGGATAACATTACAAGGTGAGACCCCGAGGGAACCATGATACAGGAAAACCAGGTTTTGCTAGTGTAGGGATAACAGGGTAATGCCCCTCTTGGGGCGTTACAC,415,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000455.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000455.png,,,,,681a12a5,False
2203,BBF10K_000456,PpHR3'-NTS,Pichia NTS 5'-Homology Region,Open Yeast Collection,pOpen-PpHR5'-NTS,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia NTS locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAGACAGAGCACTTGAATCCACTGCTGACTTTTCAAATATTTAGGGTATTTTTCTCACTTTTTCCTAGCAAACTCCAATTGGTGGGTTCAGTGCAATGGAGTATCACCTTGCAACCACAACGTAATAGCTAACTTGTGGCCACCATGTCTGGTTGTAGAGATAATTGGATTCTAATGTGGATCACATGACTACTCACGTGTCAAAAACCCAACCTGACTTGGCCCAGCTTAGCAAGAATATTTCGAATCCACTCTTGTGGCCTAGTGGACGCGGCCGCACGAATGAGACCCTGACACAGATATCCCGGGAGGAAGATATAGCACGGACAAAGTCATTCCTTCGGATAGGGATAACAGGGTAATCTAGCTGTATAGGGCGCCGTC,2411,AACGATGATGCTCACTCTCGGGTCTCTAGACAGAGCACTTGAATCCACTGCTGACTTTTCAAATATTTAGGGTATTTTTCTCACTTTTTCCTAGCAAACTCCAATTGGTGGGTTCAGTGCAATGGAGTATCACCTTGCAACCACAACGTAATAGCTAACTTGTGGCCACCATGTCTGGTTGTAGAGATAATTGGATTCTAATGTGGATCACATGACTACTCACGTGTCAAAAACCCAACCTGACTTGGCCCAGCTTAGCAAGAATATTTCGAATCCACTCTTGTGGCCTAGTGGACGCGGCCGCACGAATGAGACCCTGACACAGATATCCCGGGAGGAAGATATAGCACGGACAAAGTCATTCCTTCGGATAGGGATAACAGGGTAATCTAGCTGTATAGGGCGCCGTC,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000456.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000456.png,,,,,5e6f8218,False
2204,BBF10K_000457,PpHR5'-RGI2,Pichia RGI2 3'-Homology Region,Open Yeast Collection,pOpen-PpHR3'-RGI2,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia RGI2 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAAAAAGCGGCCGCATACCCAGCATTGACCTTTGGTATGAGCATCTGAAAAACAACCAGGTGTTGCAAAGTTAAACATCCTTCTTTGTTCATATAGAACCCACTATTCATGGTACTCCCCAATCGAATTTCACATTCTGGTTTTGAAATTACACACCACGTTAGCTTATAAGATTTCATATAACTTATTGATATACGGTTTCCATTGTTCGAATAGTTGAGGTTGTATGTAATTCGATTGAAGGGGCCATTTTTGTTTCCTACTTTTCCTGGGAGCTTATCCGATGCGCTTCAAAGCTGGAATTGTAAATATAGAGAAAAAGAAGGATGTTGTTTTATTCTTGAAAGAGTATAATTTTACTTCTAGCAACTCTCCCACTTCGCTTGACTTCATTTATTTCTTGGGCACATAGGCGTAGTAATCTAGACCAACAGATAATTTGCCGGAATGATATAGCGATTGGAAAATGAACTGAAATTTTTTGCTGTCTTTCAATTTGACGGGCAGTTCATCAGGACATAATAGTAGCCAGCTCAATGAAGGTGAGACCCCGGTTGTACCTATCGAGTGCTTGATTACCGCACCTACCCATAGGGATAACAGGGTAATGCGATTCTCATAATCCGAAGA,2657,AACGATGATGCTCACTCTCGGGTCTCTAAAAAGCGGCCGCATACCCAGCATTGACCTTTGGTATGAGCATCTGAAAAACAACCAGGTGTTGCAAAGTTAAACATCCTTCTTTGTTCATATAGAACCCACTATTCATGGTACTCCCCAATCGAATTTCACATTCTGGTTTTGAAATTACACACCACGTTAGCTTATAAGATTTCATATAACTTATTGATATACGGTTTCCATTGTTCGAATAGTTGAGGTTGTATGTAATTCGATTGAAGGGGCCATTTTTGTTTCCTACTTTTCCTGGGAGCTTATCCGATGCGCTTCAAAGCTGGAATTGTAAATATAGAGAAAAAGAAGGATGTTGTTTTATTCTTGAAAGAGTATAATTTTACTTCTAGCAACTCTCCCACTTCGCTTGACTTCATTTATTTCTTGGGCACATAGGCGTAGTAATCTAGACCAACAGATAATTTGCCGGAATGATATAGCGATTGGAAAATGAACTGAAATTTTTTGCTGTCTTTCAATTTGACGGGCAGTTCATCAGGACATAATAGTAGCCAGCTCAATGAAGGTGAGACCCCGGTTGTACCTATCGAGTGCTTGATTACCGCACCTACCCATAGGGATAACAGGGTAATGCGATTCTCATAATCCGAAGA,656,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000457.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000457.png,,,,,e5d4dd17,False
2205,BBF10K_000458,PpHR3'-RGI2,Pichia RGI2 5'-Homology Region,Open Yeast Collection,pOpen-PpHR5'-RGI2,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia RGI2 locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAACGATGATGCTCACTCTCGGGTCTCTAGACCAATATCTCTTGTCGCCATCGGGTGACCGACCATATAAATACGTTGAGAATGTTATTCTTCCTCGTAGTTGAAGTGGCTTCATAATTTCAGAACTCAATAGATAAACTAGGATGTTTTAAAGCAATTAATGCTCACAAGTAAGGAGCGACTCTCTTGCTTTTCGAATACTAAAAGTATCGTCCCAACCCAGAAAAAAAGACCTCTTAACTGCAAAATAAACTCTATATATTTCTTCTAAAACAGTTTCAGGTTGGATAGTATCGCATTCTCATCACTTCTAACTAGTAGGCCATGAGATATATTAACGTTTACTTGAGTTCTAAGTTCTCCGAATTAGATGCACAGCACAAACAAGATTAGGTTTCACTTGGTACAAAATACGAACAGAGTTTAAGGTCGTAATTTCATTTCGTTATTGATCCCCACAATCTATTCTTATCACAGTCATCAGATAGTCGCGAAAAAGCATGCAGAAAAGGGGGTCGTCCCTATCTAAGTTGTAGCATTACAACAAATATGACGCGGCCGCACGAATGAGACCCCGTGCGACAAGATTTCAAGTCGGGAAACCAGCCACCTTGATAGGGATAACAGGGTAATAGCTGTCAACTTCAGACCTTG,2654,AACGATGATGCTCACTCTCGGGTCTCTAGACCAATATCTCTTGTCGCCATCGGGTGACCGACCATATAAATACGTTGAGAATGTTATTCTTCCTCGTAGTTGAAGTGGCTTCATAATTTCAGAACTCAATAGATAAACTAGGATGTTTTAAAGCAATTAATGCTCACAAGTAAGGAGCGACTCTCTTGCTTTTCGAATACTAAAAGTATCGTCCCAACCCAGAAAAAAAGACCTCTTAACTGCAAAATAAACTCTATATATTTCTTCTAAAACAGTTTCAGGTTGGATAGTATCGCATTCTCATCACTTCTAACTAGTAGGCCATGAGATATATTAACGTTTACTTGAGTTCTAAGTTCTCCGAATTAGATGCACAGCACAAACAAGATTAGGTTTCACTTGGTACAAAATACGAACAGAGTTTAAGGTCGTAATTTCATTTCGTTATTGATCCCCACAATCTATTCTTATCACAGTCATCAGATAGTCGCGAAAAAGCATGCAGAAAAGGGGGTCGTCCCTATCTAAGTTGTAGCATTACAACAAATATGACGCGGCCGCACGAATGAGACCCCGTGCGACAAGATTTCAAGTCGGGAAACCAGCCACCTTGATAGGGATAACAGGGTAATAGCTGTCAACTTCAGACCTTG,653,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000458.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000458.png,,,,,3dfa75ed,False
2206,BBF10K_000459,PpHR5'-ENO,Pichia ENO 3'-Homology Region,Open Yeast Collection,pOpen-PpHR3'-ENO,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia ENO locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTAAAAAGCGGCCGCAAGACCAGCTTCGCTCTTAATTAAGCGGCCCAAAGAGTGAGAGGAAAGTACCTGGGCAAAATCACACAATTCCAAACCATGCTAAATGAGATTTAAAGAGCAAACGATGGCAAAAGGCAACCGTTATAAATGTGATCTTTCTTGGCAGCTATCTGTCAATTTTTCTAAGGAACAGTGAATTCATCATAGGAGAGATGTTATACGTTACATAATCATACATACTGCATGTATCTCACCTACTTTACCTCATCAACTCTAAAACAGTTCTAGTCCCAACCCCAGATTCCTAGTCATGACACAAGTCCGCACCGGACAGGACTCACAACCAGCAAGAGAAGCTAACAAATTTACGCCCCGGTAAAACATTCTTTAGGGGCCGTTCAATGGTAATTTTCCTCTCACCCGTTTAAACTTACCTCCGGGCGGTATCTTCAATAACCTCTGTTGTCCCCGGGTATCATTGGAAACAGTGAGGGACGTTGAACAGAAGAGAGGATCATCACGGGCAAGGATTGTAAAGGTGAGACCAAGACGATCCGAGCCATTACGGGGCTGAGCACCGTGAAGGTTAGGGATAACAGGGTAATACACTTTTGGTCTTATGATTT,2649,AAGAATTACTGACCCCTCGGGGTCTCTAAAAAGCGGCCGCAAGACCAGCTTCGCTCTTAATTAAGCGGCCCAAAGAGTGAGAGGAAAGTACCTGGGCAAAATCACACAATTCCAAACCATGCTAAATGAGATTTAAAGAGCAAACGATGGCAAAAGGCAACCGTTATAAATGTGATCTTTCTTGGCAGCTATCTGTCAATTTTTCTAAGGAACAGTGAATTCATCATAGGAGAGATGTTATACGTTACATAATCATACATACTGCATGTATCTCACCTACTTTACCTCATCAACTCTAAAACAGTTCTAGTCCCAACCCCAGATTCCTAGTCATGACACAAGTCCGCACCGGACAGGACTCACAACCAGCAAGAGAAGCTAACAAATTTACGCCCCGGTAAAACATTCTTTAGGGGCCGTTCAATGGTAATTTTCCTCTCACCCGTTTAAACTTACCTCCGGGCGGTATCTTCAATAACCTCTGTTGTCCCCGGGTATCATTGGAAACAGTGAGGGACGTTGAACAGAAGAGAGGATCATCACGGGCAAGGATTGTAAAGGTGAGACCAAGACGATCCGAGCCATTACGGGGCTGAGCACCGTGAAGGTTAGGGATAACAGGGTAATACACTTTTGGTCTTATGATTT,648,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000459.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000459.png,,,,,995d1d4d,False
2207,BBF10K_000460,PpHR3'-ENO,Pichia ENO 5'-Homology Region,Open Yeast Collection,pOpen-PpHR5'-ENO,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia ENO locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTAGACACTCGTATCGCATGTCGGTGCACCGTAAATTTGCCTTGCAATTGGCCCTAACCACGGATGGTTAACTTCAAGCCATCACGACAGCAATTGAGTCGGCGCATAGCTACCCTCCTCTTCTTGACCCCATGCATAGGACCAACCTTAACCGATGGAACAGGTTCCTCCGCTCCGTCCCCTGGTAGTGTCTCTGCGCAAGAAATAGTTAAGGTATTAACACTGATCTCTCGCACCCCCCTCACAGTACTGTTATGGTGAATTGACAAAGCCATTGGCTAGATTGAAACATGTAATTCATATGTAATCTTGTTCAATTAACGAGCTTCGTACAGTCTCAATCTAGACGTCTGATAATGGCGTTTGTGCTCCTAATCGATGAGCCATCTCATGTGACGTCTATACGCTTCGATGGCTTCCGTCGCGAATATAGAACCACTTGAAATATGCTGCAAACCACGATCCACCCTGGTCCTGAAAAGATATAAATACAGCACATCTAGCAGGCTTTTGCGCTGCGCGGCCGCACGAATGAGACCAAGGAACTATGGCATCGAGCGTTATGGGCGCCGGACGCTACTAGGGATAACAGGGTAATATTGCGACACCCATCTCCAGC,2645,AAGAATTACTGACCCCTCGGGGTCTCTAGACACTCGTATCGCATGTCGGTGCACCGTAAATTTGCCTTGCAATTGGCCCTAACCACGGATGGTTAACTTCAAGCCATCACGACAGCAATTGAGTCGGCGCATAGCTACCCTCCTCTTCTTGACCCCATGCATAGGACCAACCTTAACCGATGGAACAGGTTCCTCCGCTCCGTCCCCTGGTAGTGTCTCTGCGCAAGAAATAGTTAAGGTATTAACACTGATCTCTCGCACCCCCCTCACAGTACTGTTATGGTGAATTGACAAAGCCATTGGCTAGATTGAAACATGTAATTCATATGTAATCTTGTTCAATTAACGAGCTTCGTACAGTCTCAATCTAGACGTCTGATAATGGCGTTTGTGCTCCTAATCGATGAGCCATCTCATGTGACGTCTATACGCTTCGATGGCTTCCGTCGCGAATATAGAACCACTTGAAATATGCTGCAAACCACGATCCACCCTGGTCCTGAAAAGATATAAATACAGCACATCTAGCAGGCTTTTGCGCTGCGCGGCCGCACGAATGAGACCAAGGAACTATGGCATCGAGCGTTATGGGCGCCGGACGCTACTAGGGATAACAGGGTAATATTGCGACACCCATCTCCAGC,644,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000460.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000460.png,,,,,6668a2c6,False
2208,BBF10K_000461,PpHR5'-AOXTT,Pichia AOXTT 3'-Homology Region,Open Yeast Collection,pOpen-PpHR3'-AOXTT,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia AOXTT locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AAAA,AAGG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTAAAAAGCGGCCGCAGCAACGTTGTCACTGAAGTTGGCATCAGTATCTACAAACCCTACATACCTCTTGAAGGTCCAGAAGGTAAGCATTTGCCCGCTCCATTCCTACCCGACATATCCACTCTACATTTTATAGTTCAAGGCACCGAAAAAGTTCGAAACAAGAAGTTTGTTCCTGATAACAAGGATTTCTTTATTGGTGGGACTTCATTTACTGTCTCCAAGAAGGACATTTCTGCTGTCATAACCGAGATAGTCTCACAGTTCGAGTCTACTGATGACCAAAAGTCAGAGAAATTTACCATGAAATCTCCTCCTCCGGTTGCTTTAGTTGGGCATAATCTGATAGGAGGCCTCAAGACACTGAAGAATGCGGGTATCACCATTCCCATACTTCCAATCATAGGCCGTAAGTTCCATCGGTGTAAGGTGAGACCAAGGACTGCATACCAGGTTGGTATGGGTCGTACGGAAATTATAGGGATAACAGGGTAATGGATACAGGGGAGGCAACAGG,2543,AAGAATTACTGACCCCTCGGGGTCTCTAAAAAGCGGCCGCAGCAACGTTGTCACTGAAGTTGGCATCAGTATCTACAAACCCTACATACCTCTTGAAGGTCCAGAAGGTAAGCATTTGCCCGCTCCATTCCTACCCGACATATCCACTCTACATTTTATAGTTCAAGGCACCGAAAAAGTTCGAAACAAGAAGTTTGTTCCTGATAACAAGGATTTCTTTATTGGTGGGACTTCATTTACTGTCTCCAAGAAGGACATTTCTGCTGTCATAACCGAGATAGTCTCACAGTTCGAGTCTACTGATGACCAAAAGTCAGAGAAATTTACCATGAAATCTCCTCCTCCGGTTGCTTTAGTTGGGCATAATCTGATAGGAGGCCTCAAGACACTGAAGAATGCGGGTATCACCATTCCCATACTTCCAATCATAGGCCGTAAGTTCCATCGGTGTAAGGTGAGACCAAGGACTGCATACCAGGTTGGTATGGGTCGTACGGAAATTATAGGGATAACAGGGTAATGGATACAGGGGAGGCAACAGG,542,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000461.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000461.png,,,,,9e31bf82,False
2209,BBF10K_000462,PpHR3'-AOXTT,Pichia AOXTT 5'-Homology Region,Open Yeast Collection,pOpen-PpHR5'-AOXTT,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),for stable genome integration adjacent to Pichia AOXTT locus.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,homology-region,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTAGACTATCGTGTTGCATCTGCGGCTCGAGAATTATGGCTTAATCAAGTGAATACATCAAAGTCAAACTTAAAATACATTCTTCGCAGGCTTGGCTTGCCACATAGTTTTCTTCACAATGCTGCAAATGACGCTTATTATACCCTTTTGGCATCGTTGAAGCTTGCACAAACGAACGTCCCACTTAATCTTCTGTACTCTGAAGAGGAGTGGGAAATACCAAGAAAAACATCAAACTCGAATGATTTTCCCGAACCCCTACCACAAGATATTCATCAGCTGCGAGATAGGCTGATCAGGAGCAAGCTCGTACGAGAAGAAACAAAATGACAAAAAAAATCCTATACTATATAGGTTACAAATAAAAAAGTATCAAAAATGAAGCCTGCATCTCTCAGGCAAATGGCATTCTGACATCCTCTGCGGCCGCACGAATGAGACCAAGGATATGTAGACACCGCCCCCGGTGTGCTAGGATGGCTGTAGGGATAACAGGGTAATGGCTTCGCGGGATTTTAACTT,2548,AAGAATTACTGACCCCTCGGGGTCTCTAGACTATCGTGTTGCATCTGCGGCTCGAGAATTATGGCTTAATCAAGTGAATACATCAAAGTCAAACTTAAAATACATTCTTCGCAGGCTTGGCTTGCCACATAGTTTTCTTCACAATGCTGCAAATGACGCTTATTATACCCTTTTGGCATCGTTGAAGCTTGCACAAACGAACGTCCCACTTAATCTTCTGTACTCTGAAGAGGAGTGGGAAATACCAAGAAAAACATCAAACTCGAATGATTTTCCCGAACCCCTACCACAAGATATTCATCAGCTGCGAGATAGGCTGATCAGGAGCAAGCTCGTACGAGAAGAAACAAAATGACAAAAAAAATCCTATACTATATAGGTTACAAATAAAAAAGTATCAAAAATGAAGCCTGCATCTCTCAGGCAAATGGCATTCTGACATCCTCTGCGGCCGCACGAATGAGACCAAGGATATGTAGACACCGCCCCCGGTGTGCTAGGATGGCTGTAGGGATAACAGGGTAATGGCTTCGCGGGATTTTAACTT,547,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000462.gb,Scott Pownall & Isaac Larkin,https://pubmed.ncbi.nlm.nih.gov/29221460/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000462.png,,,,,30f20aab,False
2210,BBF10K_000463,Sc2micron,S. cerevisiae target ori 2micron origin,Open Yeast Collection,pOpen-Sc2micron,pBuild,M13 For,M13 Rev,,,,,,,S. cerevisiae,yeast,high copy yeast episome,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,origin,AAAA,AACG,taactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaggcctactattagcaacaacgatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaaccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacctgcaccagtcagtaaaacgacggccagtagtcaaaagcctccgaccggaggcttttgacttggttcaggtggagtgggagtagtcttcaagaattactgacccctcggggtctctaaaacctaggaacgaagcatctgtgcttcattttgtagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtccggtgcgtttttggttttttgaaagtgcgtcatcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagctagagaataggaacttcaccggtaaggtgagaccaaggcccagaaggatacaactagcactgcacattaaaagactagggataacagggtaattgtagcgtcgtctaattctcagaagaccacgctgaggtgtcaatcgtcggagccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgcatggtcatagctgtttcctgagagcttggcaggtgatgacacacattaacaaatttcgtgaggagtctccagaagaatgccattaatttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccgg,3152,AAGAATTACTGACCCCTCGGGGTCTCtAAAACCTAGGaacgaagcatctgtgcttcattttgtagaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgaaagcgctattttaccaacgaagaatctgtgcttcatttttgtaaaacaaaaatgcaacgcgagagcgctaatttttcaaacaaagaatctgagctgcatttttacagaacagaaatgcaacgcgagagcgctattttaccaacaaagaatctatacttcttttttgttctacaaaaatgcatcccgagagcgctatttttctaacaaagcatcttagattactttttttctcctttgtgcgctctataatgcagtctcttgataactttttgcactgtaggtccgttaaggttagaagaaggctactttggtgtctattttctcttccataaaaaaagcctgactccacttcccgcgtttactgattactagcgaagctgcgggtgcattttttcaagataaaggcatccccgattatattctataccgatgtggattgcgcatactttgtgaacagaaagtgatagcgttgatgattcttcattggtcagaaaattatgaacggtttcttctattttgtctctatatactacgtataggaaatgtttacattttcgtattgttttcgattcactctatgaatagttcttactacaatttttttgtctaaagagtaatactagagataaacataaaaaatgtagaggtcgagtttagatgcaagttcaaggagcgaaaggtggatgggtaggttatatagggatatagcacagagatatatagcaaagagatacttttgagcaatgtttgtggaagcggtattcgcaatattttagtagctcgttacagtccggtgcgtttttggttttttgaaagtgcgtcatcagagcgcttttggttttcaaaagcgctctgaagttcctatactttctagctagagaataggaacttcACCGGTAAGGtGAGACCAAGGCCCAGAAGGATACAACTAGCACTGCACATTAAAAGACTAGGGATAACAGGGTAATTGTAGCGTCGTCTAATTCTCA,1135,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000463.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC216781/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000463.png,,,,,585abb46,False
2211,BBF10K_000464,ScARS_CEN,S. cerevisiae target ori ARS_CEN,Open Yeast Collection,pOpen-ScARS_CEN,pBuild,M13 For,M13 Rev,,,,,,,S. cerevisiae,yeast,low copy yeast episome,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,origin,AAAA,AACG,AAGAATTACTGACCCCTCGGGGTCTCTAAAACCTAGGATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTCCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAACACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCAAGGTGAGACCACTAGTAAGGCGCTCGGATAATACTCAAGTTCAGTCTTGTTAAAGTCTAGGGATAACAGGGTAATCCGCTCCAGCACGCTGATGACCGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAG,2639,AAGAATTACTGACCCCTCGGGGTCTCtAAAACCTAGGatcacgtgctataaaaataattataatttaaattttttaatataaatatataaattaaaaatagaaagtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactctagggggatcgccaacaaatactaccttttatcttCctcttcctgctctcaggtattaatgccgaattgtttcatcttgtctgtgtagaaCaccacacacgaaaatcctgtgattttacattttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatatatatgtaaagtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcattccgtaactcttctaccttctttatttactttctaaaatccaaatacaaaacataaaaataaataaacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgtaagttacaggcaagcgatcACCGGTAAGGtGAGACCAAGGCGCTCGGATAATACTCAAGTTCAGTCTTGTTAAAGTCTAGGGATAACAGGGTAATCCGCTCCAGCACGCTGATGAC,638,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000464.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000464.png,,,,,3971ff07,False
2212,BBF10K_000465,Pichia PARS,Pichia (Komagataella pastoris) origin,Open Yeast Collection,pOpen-PpPARS,pBuild,M13 For,M13 Rev,,,,,,,Pichia (Komagataella pastoris),Pichia (Komagataella pastoris),Pichia (Komagataella pastoris) episome,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,origin,AAAA,AACG,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTAAAACCTAGGCCTTCGTTTGTGCGGATCCAATTAATATTTACTTATTTTGGTCAACCCCAAATAGGTTGATTTCATACTTGGTTCATTCAAAAATAAGTAGTCTTTTGAGATCTTTCAATATTATAATAAATATACTATAACAGCCGACTTGTTTCATTTTCGCGAATGTTCCCCCAGCTTATCGACCGGTAAGGTGAGACCGTCGATCAACCAGTTTCGATCTGAACTAATAACAGCCCATTCACGTGGTAGCGGTGGAAAATAGACGACTAATTGAAACGGATATGATACCGACAGATATCAAGGTATGTATAGCGACCGCGGCGGTCGTATTTGATTCAAGTAGGGATAACAGGGTAATTCTTTAGCTAGGAGAAATCTA,2411,AAGAATTACTGACCCCTCGGGGTCTCTAAAACCTAGGCCTTCGTTTGTGCGGATCCAATTAATATTTACTTATTTTGGTCAACCCCAAATAGGTTGATTTCATACTTGGTTCATTCAAAAATAAGTAGTCTTTTGAGATCTTTCAATATTATAATAAATATACTATAACAGCCGACTTGTTTCATTTTCGCGAATGTTCCCCCAGCTTATCGACCGGTAAGGTGAGACCGTCGATCAACCAGTTTCGATCTGAACTAATAACAGCCCATTCACGTGGTAGCGGTGGAAAATAGACGACTAATTGAAACGGATATGATACCGACAGATATCAAGGTATGTATAGCGACCGCGGCGGTCGTATTTGATTCAAGTAGGGATAACAGGGTAATTCTTTAGCTAGGAGAAATCTA,410,ColE1 High Copy,500-701,BSL2,,,https://freegenes.github.io/genbank/BBF10K_000465.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000465.png,,,,,ae4cb0c5,False
2213,BBF10K_000466,oriT,S. cerevisiae target oriT,Open Yeast Collection,pOpen-oriT,pBuild,M13 For,M13 Rev,,,,,,,Pseudomonas aeruginosa,yeast,Trans-kingdom DNA transfer. The origin of transfer (oriT) is necessary for transfer of the DNA that contains it from a bacterial host to recipient during bacterial conjugation. The oriT is cis-acting.,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,transfer-origin,CGAA,GCAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCTCGAACCTAGGGCGATCGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAGATACCGGTGCAATGAGACCAATAGGAACCTCTTACGCGGAGAAGGGTAAGGGCCACTTATTAGGGATAACAGGGTAATTCTCGTGGACTCGAGGGGGGG,2913,AAGAATTACTGACCCCTCGGGGTCTCTCGAACCTAGGGCGATCGTCTTGCCTTGCTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGCGCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAGCTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAGATACCGGTGCAATGAGACCAATAGGAACCTCTTACGCGGAGAAGGGTAAGGGCCACTTATTAGGGATAACAGGGTAATTCTCGTGGACTCGAGGGGGGG,912,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000466.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC207219/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000466.png,,,,,f8f4128c,False
2214,BBF10K_000467,ScADE2-marker,S. cerevisiae marker-cassette-ADE2,Open Yeast Collection,pOpen-ScADE2-marker,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000005654,,,,"Yeast selection marker, complements ADE2 auxotrophy","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACGTGCAGGAAACGAAGATAAATCATGGATTCTAGAACAGTTGGTATATTAGGAGGGGGACAATTGGGACGTATGATTGTTGAGGCAGCAAACAGGCTCAACATTAAGACGGTAATACTAGATGCTGAAAATTCTCCTGCCAAACAAATAAGCAACTCCAATGACCACGTTAATGGCTCCTTTTCCAATCCTCTTGATATCGAAAAACTAGCTGAAAAATGTGATGTGCTAACGATTGAGATTGAGCATGTTGATGTTCCTACACTAAAGAATCTTCAAGTAAAACATCCCAAATTAAAAATTTACCCTTCTCCAGAAACAATCAGATTGATACAAGACAAATATATTCAAAAAGAGCATTTAATCAAAAATGGTATAGCAGTTACCCAAAGTGTTCCTGTGGAACAAGCCAGTGAGACGTCCCTATTGAATGTTGGAAGAGATTTGGGTTTTCCATTCGTCTTGAAGTCGAGGACTTTGGCATACGATGGAAGAGGTAACTTCGTTGTAAAGAATAAGGAAATGATTCCGGAAGCTTTGGAAGTACTGAAGGATCGTCCTTTGTACGCCGAAAAATGGGCACCATTTACTAAAGAATTAGCAGTCATGATTGTGAGATCTGTTAACGGTTTAGTGTTTTCTTACCCAATTGTAGAGACTATCCACAAGGACAATATTTGTGACTTATGTTATGCGCCTGCTAGAGTTCCGGACTCCGTTCAACTTAAGGCGAAGTTGTTGGCAGAAAATGCAATCAAATCTTTTCCCGGTTGTGGTATATTTGGTGTGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCCCCAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTCTCAATTTGAAGCTCATTTGAGATCAATATTGGATTTGCCAATGCCAAAGAATTTCACATCTTTCTCCACCATTACAACGAACGCCATTATGCTAAATGTTCTTGGAGACAAACATACAAAAGATAAAGAGCTAGAAACTTGCGAAAGAGCATTGGCGACTCCAGGTTCCTCAGTGTACTTATATGGAAAAGAGTCTAGACCTAACAGAAAAGTAGGTCACATAAATATTATTGCCTCCAGTATGGCGGAATGTGAACAAAGGCTGAACTACATTACAGGTAGAACTGATATTCCAATCAAAATCTCTGTCGCTCAAAAGTTGGACTTGGAAGCAATGGTCAAACCATTGGTTGGAATCATCATGGGATCAGACTCTGACTTGCCGGTAATGTCTGCCGCATGTGCGGTTTTAAAAGATTTTGGCGTTCCATTTGAAGTGACAATAGTCTCTGCTCATAGAACTCCACATAGGATGTCAGCATATGCTATTTCCGCAAGCAAGCGTGGAATTAAAACAATTATCGCTGGAGCTGGTGGGGCTGCTCACTTGCCAGGTATGGTGGCTGCAATGACACCACTTCCTGTCATCGGTGTGCCCGTAAAAGGTTCTTGTCTAGATGGAGTAGATTCTTTACATTCAATTGTGCAAATGCCTAGAGGTGTTCCAGTAGCTACCGTCGCTATTAATAATAGTACGAACGCTGCGCTGTTGGCTGTCAGACTGCTTGGCGCTTATGATTCAAGTTATACAACGAAAATGGAACAGTTTTTATTAAAGCAAGAAGAAGAAGTTCTTGTCAAAGCACAAAAGTTAGAAACTGTCGGTTACGAAGCTTATCTAGAAAACAAGTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCATGATGAGACCAATATCACGCAAAAGCACCGGCAGTCGAAATTCTCGACGATTAGGGATAACAGGGTAATAGCTGTGATCCGGAATTTGTT,4094,AAGAATTACTGACCCCTCGGGGTCTCAAAGGCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACGTGCAGGAAACGAAGATAAATCATGGATTCTAGAACAGTTGGTATATTAGGAGGGGGACAATTGGGACGTATGATTGTTGAGGCAGCAAACAGGCTCAACATTAAGACGGTAATACTAGATGCTGAAAATTCTCCTGCCAAACAAATAAGCAACTCCAATGACCACGTTAATGGCTCCTTTTCCAATCCTCTTGATATCGAAAAACTAGCTGAAAAATGTGATGTGCTAACGATTGAGATTGAGCATGTTGATGTTCCTACACTAAAGAATCTTCAAGTAAAACATCCCAAATTAAAAATTTACCCTTCTCCAGAAACAATCAGATTGATACAAGACAAATATATTCAAAAAGAGCATTTAATCAAAAATGGTATAGCAGTTACCCAAAGTGTTCCTGTGGAACAAGCCAGTGAGACGTCCCTATTGAATGTTGGAAGAGATTTGGGTTTTCCATTCGTCTTGAAGTCGAGGACTTTGGCATACGATGGAAGAGGTAACTTCGTTGTAAAGAATAAGGAAATGATTCCGGAAGCTTTGGAAGTACTGAAGGATCGTCCTTTGTACGCCGAAAAATGGGCACCATTTACTAAAGAATTAGCAGTCATGATTGTGAGATCTGTTAACGGTTTAGTGTTTTCTTACCCAATTGTAGAGACTATCCACAAGGACAATATTTGTGACTTATGTTATGCGCCTGCTAGAGTTCCGGACTCCGTTCAACTTAAGGCGAAGTTGTTGGCAGAAAATGCAATCAAATCTTTTCCCGGTTGTGGTATATTTGGTGTGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCCCCAAGGCCTCACAACTCTGGACATTATACCATTGATGCTTGCGTCACTTCTCAATTTGAAGCTCATTTGAGATCAATATTGGATTTGCCAATGCCAAAGAATTTCACATCTTTCTCCACCATTACAACGAACGCCATTATGCTAAATGTTCTTGGAGACAAACATACAAAAGATAAAGAGCTAGAAACTTGCGAAAGAGCATTGGCGACTCCAGGTTCCTCAGTGTACTTATATGGAAAAGAGTCTAGACCTAACAGAAAAGTAGGTCACATAAATATTATTGCCTCCAGTATGGCGGAATGTGAACAAAGGCTGAACTACATTACAGGTAGAACTGATATTCCAATCAAAATCTCTGTCGCTCAAAAGTTGGACTTGGAAGCAATGGTCAAACCATTGGTTGGAATCATCATGGGATCAGACTCTGACTTGCCGGTAATGTCTGCCGCATGTGCGGTTTTAAAAGATTTTGGCGTTCCATTTGAAGTGACAATAGTCTCTGCTCATAGAACTCCACATAGGATGTCAGCATATGCTATTTCCGCAAGCAAGCGTGGAATTAAAACAATTATCGCTGGAGCTGGTGGGGCTGCTCACTTGCCAGGTATGGTGGCTGCAATGACACCACTTCCTGTCATCGGTGTGCCCGTAAAAGGTTCTTGTCTAGATGGAGTAGATTCTTTACATTCAATTGTGCAAATGCCTAGAGGTGTTCCAGTAGCTACCGTCGCTATTAATAATAGTACGAACGCTGCGCTGTTGGCTGTCAGACTGCTTGGCGCTTATGATTCAAGTTATACAACGAAAATGGAACAGTTTTTATTAAAGCAAGAAGAAGAAGTTCTTGTCAAAGCACAAAAGTTAGAAACTGTCGGTTACGAAGCTTATCTAGAAAACAAGTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCATGATGAGACCAATATCACGCAAAAGCACCGGCAGTCGAAATTCTCGACGATTAGGGATAACAGGGTAATAGCTGTGATCCGGAATTTGTT,2093,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000467.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000467.png,,,,,8ad68696,False
2215,BBF10K_000468,ScHIS3-marker,S. cerevisiae marker-cassette-HIS3,Open Yeast Collection,pOpen-ScHIS3-marker,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000005728,,,,"Yeast selection marker, complements HIS3 auxotrophy","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATTACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTTTTATTTTTCCACCTAGCGGATGACTCTTATTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATAAACGAAGGCAAAGATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGATCTTCCCAGAAAAAGAGGCAGAAGCAGTAGCAGAACAGGCCACACAATCGCAAGTGATTAACGTCCACACAGGTATAGGGTTTCTGGACCATATGATACATGCTCTGGCCAAGCATTCCGGCTGGTCGCTAATCGTTGAGTGCATTGGTGACTTACACATAGACGACCATCACACCACTGAGGACTGCGGGATTGCTCTCGGTCAAGCTTTTAAAGAGGCCCTAGGGGCCGTGCGTGGAGTAAAAAGGTTTGGATCAGGATTTGCGCCTTTGGATGAGGCACTTTCCAGAGCGGTGGTTGATCTTTCGAACAGGCCGTACGCAGTTGTCGAACTTGGTTTGCAAAGGGAGAAAGTAGGTGATCTCTCTTGCGAGATGATCCCGCATTTTCTTGAAAGCTTTGCAGAGGCAAGCAGAATTACCCTCCACGTTGATTGTCTGCGAGGCAAGAATGATCATCACCGTAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAGTGACACCGATTATTTAAAGCTGCTGCATACGATATATATACATGTGTATATATGTATACCTATGAATGTCAGTAAGTATGTATACGAACAGTATGATACTGAAGATGACAAGGTAATGCATCATTCTATACGTGTCATTCTGAACGAGGCGCGCTTTCCTTTTTTCTTTTTGCTTTTTCTTTTTTTTTCTCTTGAACTCGACGGATCATAATGATGAGACCAATCAGTTTCTTTGGCAGCCGGTACCCCCGTCGACCATGGTTAGGGATAACAGGGTAATGCTTATACGCTGCTTTGGATG,3233,AAGAATTACTGACCCCTCGGGGTCTCAAAGGAACACAGTCCTTTCCCGCAATTTTCTTTTTCTATTACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATGCCTCGGTAATGATTTTCATTTTTATTTTTCCACCTAGCGGATGACTCTTATTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAAAGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATAAACGAAGGCAAAGATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGATCTTCCCAGAAAAAGAGGCAGAAGCAGTAGCAGAACAGGCCACACAATCGCAAGTGATTAACGTCCACACAGGTATAGGGTTTCTGGACCATATGATACATGCTCTGGCCAAGCATTCCGGCTGGTCGCTAATCGTTGAGTGCATTGGTGACTTACACATAGACGACCATCACACCACTGAGGACTGCGGGATTGCTCTCGGTCAAGCTTTTAAAGAGGCCCTAGGGGCCGTGCGTGGAGTAAAAAGGTTTGGATCAGGATTTGCGCCTTTGGATGAGGCACTTTCCAGAGCGGTGGTTGATCTTTCGAACAGGCCGTACGCAGTTGTCGAACTTGGTTTGCAAAGGGAGAAAGTAGGTGATCTCTCTTGCGAGATGATCCCGCATTTTCTTGAAAGCTTTGCAGAGGCAAGCAGAATTACCCTCCACGTTGATTGTCTGCGAGGCAAGAATGATCATCACCGTAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAGTGACACCGATTATTTAAAGCTGCTGCATACGATATATATACATGTGTATATATGTATACCTATGAATGTCAGTAAGTATGTATACGAACAGTATGATACTGAAGATGACAAGGTAATGCATCATTCTATACGTGTCATTCTGAACGAGGCGCGCTTTCCTTTTTTCTTTTTGCTTTTTCTTTTTTTTTCTCTTGAACTCGACGGATCATAATGATGAGACCAATCAGTTTCTTTGGCAGCCGGTACCCCCGTCGACCATGGTTAGGGATAACAGGGTAATGCTTATACGCTGCTTTGGATG,1232,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000468.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000468.png,,,,,ad052e22,False
2216,BBF10K_000469,ScHygro-marker,S. cerevisiae marker-cassette-Hygro,Open Yeast Collection,pOpen-ScHygro-marker,pBuild,M13 For,M13 Rev,,,,,,,,,Yeast antibiotic selection marker,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTGTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTTAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAACCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCCATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCAATGCAAAGCTATTAGCGCGACCACATGGAGATTTAGTCCTTAGGGATAACAGGGTAATGGGGCCCAATGTCACTGGCGG,4102,AAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTGTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAATTTAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTGCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTGCAACCGGTCGCGGAGGCCATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCCATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCGCGGCTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGGAATAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCAATGCAAAGCTATTAGCGCGACCACATGGAGATTTAGTCCTTAGGGATAACAGGGTAATGGGGCCCAATGTCACTGGCGG,2101,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000469.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871405,,we are unaware,freegenes.github.io/genes/images/BBF10K_000469.png,,,,,8aa15c8f,False
2217,BBF10K_000470,ScKanR-marker,marker-cassette-KanR,Open Yeast Collection,pOpen-ScKanR-marker,pBuild,M13 For,M13 Rev,,,,,,,,,Yeast antibiotic selection marker,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGAGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTGGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCAATGCGTCATTTTACACGGCGTTCCTCAGGCCTACCGGACTTAGGGATAACAGGGTAATGGATTTTATCGCGTCAATGGC,3883,AAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGAGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTGGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCAATGCGTCATTTTACACGGCGTTCCTCAGGCCTACCGGACTTAGGGATAACAGGGTAATGGATTTTATCGCGTCAATGGC,1882,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000470.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871406,,we are unaware,freegenes.github.io/genes/images/BBF10K_000470.png,,,,,cd960580,False
2218,BBF10K_000471,ScLEU2-marker,marker-cassette-LEU2,Open Yeast Collection,pOpen-ScLEU2-marker,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000523,,,,"Yeast selection marker, complements LEU2 auxotrophy","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGATCGAGGAGAACTTCTAGTATATCTACATACCTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCAAAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATATTTCAAGGATATACCATTCTAATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGATTTCGAAAATCATTTAATTGGTGGTGCTGCTATCGATGCTACAGGTGTTCCACTTCCAGATGAGGCGCTGGAAGCCTCCAAGAAGGCTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCTAAATGGGGAACCGGTAGTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACTTAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACTTATCTCCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTAAGAGAAAGGAGGACGATGGTGATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAATGGCCGCTTTCATGGCCCTACAACATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACGAATTTCCTACATTGAAGGTTCAACATCAATTGATTGATTCTGCCGCCATGATCCTAGTTAAGAACCCAACCCACCTAAATGGTATTATAATCACCAGCAACATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAGATTTGCCAAAGAATAAGGTCAACCCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAACCTGGAGGACCCTTCTCTTTAGACTATTCTACTCTTATGCACGTAAAAAATTCTAGGAAATATGTATTAACTAGGAGTAAAATAACCGGCTAGTGGCATTCATATAGCCGTCTGTTTACATCTACATCACACATTTCGAGTGTATATCTCGCAACGTTGGCGTTAAATAGGCAGATGATGAGACCAATGTCCTTAGGCAGTCGTCTGGATGTATTCAGAACGGCGCTAGGGATAACAGGGTAATTTTGTAAAAATTAAATTTCGG,4041,AAGAATTACTGACCCCTCGGGGTCTCAAAGGATCGAGGAGAACTTCTAGTATATCTACATACCTAATATTATTGCCTTATTAAAAATGGAATCCCAACAATTACATCAAAATCCACATTCTCTTCAAAATCAATTGTCCTGTACTTCCTTGTTCATGTGTGTTCAAAAACGTTATATTTATAGGATAATTATACTCTATTTCTCAACAAGTAATTGGTTGTTTGGCCGAGCGGTCTAAGGCGCCTGATTCAAGAAATATCTTGACCGCAGTTAACTGTGGGAATACTCAGGTATCGTAAGATGCAAGAGTTCGAATCTCTTAGCAACCATTATTTTTTTCCTCAACATAACGAGAACACACAGGGGCGCTATCGCACAGAATCAAATTCGATGACTGGAAATTTTTTGTTAATTTCAGAGGTCGCCTGACGCATATACCTTTTTCAACTGAAAAATTGGGAGAAAAAGGAAAGGTGAGAGCGCCGGAACCGGCTTTTCATATAGAATAGAGAAGCGTTCATGACTAAATGCTTGCATCACAATACTTGAAGTTGACAATATTATTTAAGGACCTATTGTTTTTTCCAATAGGTGGTTAGCAATCGTCTTACTTTCTAACTTTTCTTACCTTTTACATTTCAGCAATATATATATATATATTTCAAGGATATACCATTCTAATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTCGTTCCAATGTCAAGTTCGATTTCGAAAATCATTTAATTGGTGGTGCTGCTATCGATGCTACAGGTGTTCCACTTCCAGATGAGGCGCTGGAAGCCTCCAAGAAGGCTGATGCCGTTTTGTTAGGTGCTGTGGGTGGTCCTAAATGGGGAACCGGTAGTGTTAGACCTGAACAAGGTTTACTAAAAATCCGTAAAGAACTTCAATTGTACGCCAACTTAAGACCATGTAACTTTGCATCCGACTCTCTTTTAGACTTATCTCCAATCAAGCCACAATTTGCTAAAGGTACTGACTTCGTTGTTGTCAGAGAATTAGTGGGAGGTATTTACTTTGGTAAGAGAAAGGAGGACGATGGTGATGGTGTCGCTTGGGATAGTGAACAATACACCGTTCCAGAAGTGCAAAGAATCACAAGAATGGCCGCTTTCATGGCCCTACAACATGAGCCACCATTGCCTATTTGGTCCTTGGATAAAGCTAATGTTTTGGCCTCTTCAAGATTATGGAGAAAAACTGTGGAGGAAACCATCAAGAACGAATTTCCTACATTGAAGGTTCAACATCAATTGATTGATTCTGCCGCCATGATCCTAGTTAAGAACCCAACCCACCTAAATGGTATTATAATCACCAGCAACATGTTTGGTGATATCATCTCCGATGAAGCCTCCGTTATCCCAGGTTCCTTGGGTTTGTTGCCATCTGCGTCCTTGGCCTCTTTGCCAGACAAGAACACCGCATTTGGTTTGTACGAACCATGCCACGGTTCTGCTCCAGATTTGCCAAAGAATAAGGTCAACCCTATCGCCACTATCTTGTCTGCTGCAATGATGTTGAAATTGTCATTGAACTTGCCTGAAGAAGGTAAGGCCATTGAAGATGCAGTTAAAAAGGTTTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAACCTGGAGGACCCTTCTCTTTAGACTATTCTACTCTTATGCACGTAAAAAATTCTAGGAAATATGTATTAACTAGGAGTAAAATAACCGGCTAGTGGCATTCATATAGCCGTCTGTTTACATCTACATCACACATTTCGAGTGTATATCTCGCAACGTTGGCGTTAAATAGGCAGATGATGAGACCAATGTCCTTAGGCAGTCGTCTGGATGTATTCAGAACGGCGCTAGGGATAACAGGGTAATTTTGTAAAAATTAAATTTCGG,2040,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000471.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000471.png,,,,,4a2870d3,False
2219,BBF10K_000472,ScNAT-marker,marker-cassette-NAT,Open Yeast Collection,pOpen-ScNAT-marker,pBuild,M13 For,M13 Rev,,,,,,,,,Yeast antibiotic selection marker - Nourseothricin Acetyltransferase,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTACTACCTTAGATGATACAGCCTACAGATACAGAACATCAGTCCCTGGTGATGCTGAAGCAATTGAGGCTTTAGACGGTTCATTCACCACCGACACCGTCTTTAGAGTAACCGCCACCGGTGATGGATTTACCTTAAGAGAAGTCCCAGTAGACCCTCCATTAACTAAAGTCTTTCCAGATGATGAATCTGATGACGAAAGCGACGACGGAGAAGATGGTGACCCAGATTCAAGAACTTTCGTAGCATACGGTGATGACGGTGATTTGGCTGGTTTTGTAGTCGTTTCTTATTCAGGTTGGAATAGAAGGTTGACCGTTGAAGATATAGAAGTCGCCCCAGAGCATAGAGGTCATGGTGTAGGTAGAGCTTTGATGGGTTTGGCTACAGAATTTGCAAGAGAGAGAGGAGCCGGTCATTTATGGTTAGAAGTTACTAATGTTAACGCCCCTGCTATCCATGCTTATAGAAGAATGGGTTTCACATTATGTGGTTTAGATACTGCTTTATATGATGGAACAGCATCTGACGGTGAACAGGCCTTGTATATGTCTATGCCTTGCCCTTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAACGAGCAGACCGAATAGCTTTCCCTAGACGTTGCCACATAGGGATAACAGGGTAATGGTTCCTTAGTCCTCAGACGC,3646,AAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGGTACTACCTTAGATGATACAGCCTACAGATACAGAACATCAGTCCCTGGTGATGCTGAAGCAATTGAGGCTTTAGACGGTTCATTCACCACCGACACCGTCTTTAGAGTAACCGCCACCGGTGATGGATTTACCTTAAGAGAAGTCCCAGTAGACCCTCCATTAACTAAAGTCTTTCCAGATGATGAATCTGATGACGAAAGCGACGACGGAGAAGATGGTGACCCAGATTCAAGAACTTTCGTAGCATACGGTGATGACGGTGATTTGGCTGGTTTTGTAGTCGTTTCTTATTCAGGTTGGAATAGAAGGTTGACCGTTGAAGATATAGAAGTCGCCCCAGAGCATAGAGGTCATGGTGTAGGTAGAGCTTTGATGGGTTTGGCTACAGAATTTGCAAGAGAGAGAGGAGCCGGTCATTTATGGTTAGAAGTTACTAATGTTAACGCCCCTGCTATCCATGCTTATAGAAGAATGGGTTTCACATTATGTGGTTTAGATACTGCTTTATATGATGGAACAGCATCTGACGGTGAACAGGCCTTGTATATGTCTATGCCTTGCCCTTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAACGAGCAGACCGAATAGCTTTCCCTAGACGTTGCCACATAGGGATAACAGGGTAATGGTTCCTTAGTCCTCAGACGC,1645,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000472.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871407,,we are unaware,freegenes.github.io/genes/images/BBF10K_000472.png,,,,,d181552b,False
2220,BBF10K_000473,ScTRP1-marker,marker-cassette-TRP1,Open Yeast Collection,pOpen-ScTRP1-marker,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000002414,,,,"Yeast selection marker, complements TRP1 auxotrophy","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTGGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGAGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAGACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAAGGAGTCGGCATATCACTACACGCATTTGTATGAGGGATAGGGATAACAGGGTAATTCGCTTGAATGCCGTGAAGAA,3748,AAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTGGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGAGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAGACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAAGGAGTCGGCATATCACTACACGCATTTGTATGAGGGATAGGGATAACAGGGTAATTCGCTTGAATGCCGTGAAGAA,1747,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000473.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000473.png,,,,,e181ccc2,False
2221,BBF10K_000474,ScURA3-marker,marker-cassette-URA3,Open Yeast Collection,pOpen-ScURA3-marker,pBuild,M13 For,M13 Rev,,,,,https://www.yeastgenome.org/locus/S000000747,,,,"Yeast selection marker, complements URA3 auxotrophy","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACGTGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAGGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCTAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGATGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTTTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCATGATGAGACCACAGAACGAACAGGCACTACACAGTCTTATTTTCATCGTCTTAGGGATAACAGGGTAATCACTAATCATGCCTTGCGCGC,3182,AAGAATTACTGACCCCTCGGGGTCTCAAAGGCGGTTTCCTTGAAATTTTTTTGATTCGGTAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTGGTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATTCTTAACCCAACTGCACAGAACAAAAACGTGCAGGAAACGAAGATAAATCATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAGGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCTAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGATGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTTTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAGAGCTTCAATTTAATTATATCAGTTATTACCCATGATGAGACCACAGAACGAACAGGCACTACACAGTCTTATTTTCATCGTCTTAGGGATAACAGGGTAATCACTAATCATGCCTTGCGCGC,1181,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000474.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1203683/,,we are unaware,freegenes.github.io/genes/images/BBF10K_000474.png,,,,,a1fbc251,False
2222,BBF10K_000475,ScZeo-marker,marker-cassette-Zeo,Open Yeast Collection,pOpen-ScZeo-marker,pBuild,M13 For,M13 Rev,,,,,,https://www.uniprot.org/uniprot/P17493,,,Yeast antibiotic selection marker - Bleomycin resistance protein,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,AAGG,ATGA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGCTAAATTAACATCTGCCGTTCCTGTTTTAACAGCTAGGGATGTTGCAGGAGCTGTAGAGTTTTGGACAGATAGGTTAGGATTCTCAAGAGACTTTGTTGAGGACGATTTTGCTGGTGTTGTCAGGGATGACGTTACTTTATTTATCTCAGCAGTCCAAGATCAAGTTGTCCCTGATAATACATTGGCTTGGGTCTGGGTCAGGGGTTTAGATGAATTATATGCTGAATGGTCAGAAGTTGTATCTACAAACTTCAGAGATGCTTCTGGTCCAGCTATGACCGAGATTGGTGAACAGCCATGGGGTAGAGAATTTGCTTTGAGAGATCCAGCTGGAAATTGTGTTCATTTTGTTGCTGAAGAACAAGATTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAGGAAGCAAGGTATACGCCGCTGTGTAATTTTCCTGCATTAGGGATAACAGGGTAATTTCAGGCAAGGCAGCATCAGA,3448,AAGAATTACTGACCCCTCGGGGTCTCAAAGGGTGAGTAAGGAAAGAGTGAGGAACTATCGCATACCTGCATTTAAAGATGCCGATTTGGGCGCGAATCCTTTATTTTGGCTTCACCCTCATACTATTATCAGGGCCAGAAAAAGGAAGTGTTTCCCTCCTTCTTGAATTGATGTTACCCTCATAAAGCACGTGGCCTCTTATCGAGAAAGAAATTACCGTCGCTCGTGATTTGTTTGCAAAAAGAACAAAACTGAAAAAACCCAGACACGCTCGACTTCCTGTCATCCTATTGATTGCAGCTTCCAATTTCGTCACACAACAAGGTCCTAGCGACGGCTCACAGGTTTTGTAACAAGCAATCGAAGGTTCTGGAATGGCGGGAAAGGGTTTAGTACCACATGCTATGATGCCCACTGTGATCTCCAGAGCAAAGTTCGTTCGATCGTACTGTTACTCTCTCTCTTTCAAACAGAATTGTCCGAATCGTGTGACAACAACAGCCTGTTCTCACACACTCTTTTCTTCTAACCAAGGGGGTGGTTTAGTTTAGTAGAACCTCGTGAAACTTACATTTACATATATATAAACTTGCATAAATTGGTCAATGCAAGAAATACATATTTGGTCTTTTCTAATTCGTAGTTTTTCAAGTTCTTAGATGCTTTCTTTTTCTCTTTTTTACAGATCATCAAGGAAGTAATTATCTACTTTTTACAACAAATATAAAACAATGGCTAAATTAACATCTGCCGTTCCTGTTTTAACAGCTAGGGATGTTGCAGGAGCTGTAGAGTTTTGGACAGATAGGTTAGGATTCTCAAGAGACTTTGTTGAGGACGATTTTGCTGGTGTTGTCAGGGATGACGTTACTTTATTTATCTCAGCAGTCCAAGATCAAGTTGTCCCTGATAATACATTGGCTTGGGTCTGGGTCAGGGGTTTAGATGAATTATATGCTGAATGGTCAGAAGTTGTATCTACAAACTTCAGAGATGCTTCTGGTCCAGCTATGACCGAGATTGGTGAACAGCCATGGGGTAGAGAATTTGCTTTGAGAGATCCAGCTGGAAATTGTGTTCATTTTGTTGCTGAAGAACAAGATTAAACAGATGACGGGAGACACTAGCACACAACTTTACCAGGCAAGGTATTTGACGCTAGCATGTGTCCAATTCAGTGTCATTTATGATTTTTTGTAGTAGGATATAAATATATACAGCGCTCCAAATAGTGCGGTTGCCCCAAAAACACCACGGAACCTCATCTGTTCTCGTACTTTGTTGTGACAAAGTAGCTCACTGCCTTATTATCACATTTTCATTATGCAACGCTTCGGAAAATACGATGTTGAAAATATGATGAGACCACAGGAAGCAAGGTATACGCCGCTGTGTAATTTTCCTGCATTAGGGATAACAGGGTAATTTCAGGCAAGGCAGCATCAGA,1447,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000475.gb,Scott Pownall,https://www.ncbi.nlm.nih.gov/pubmed/25871408,,we are unaware,freegenes.github.io/genes/images/BBF10K_000475.png,,,,,f9a76825,False
2223,BBF10K_000476,OYC-bridge-AGAC-CGAA,OYC-bridge-AGAC-CGAA,Open Yeast Collection,pOpen-OYC-bridge-AGAC-CGAA,pBuild,M13 For,M13 Rev,,,,,,,,,"Bridge for AGAC/CGAA, See design: https://docs.google.com/spreadsheets/d/1hhiKwaTJyWajH1fEUxZ_79DP4TRtlCBLvO6EtcqtxeY/edit?usp=sharing","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,bridge,AGAC,CGAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAGACAGATCCATCCGTTCACTGCCTAGGTGTACATAAGTCGAATACACTGGTTCCATTAGTATCTCAGGTCATTGTCACTGCTCATCGGCACAGCCACAGTATGAATCAGCGCACATAAACTCTTTAGCATCTACATATAACTCTACAGACAGATCTGGATGTACAGCGAACGAGACCGGGAGGAGCACAAAGTCCGCTCAGCTTCGCCTCGTGGGGAGAGATGCGGGATAATAAGCAACTCTAGGGCAGGAAAGTGCCTGCTGACGAATTGGTCTGGCATGGATTCCACACTATAGGATAGTACAGGGTATATTGAGTGCCCCACTGAACCTGTTGGCGGGGTTAGGGATAACAGGGTAATAACAGAATCCCCTTCGGTGTA,2411,AAGAATTACTGACCCCTCGGGGTCTCAAGACAGATCCATCCGTTCACTGCCTAGGTGTACATAAGTCGAATACACTGGTTCCATTAGTATCTCAGGTCATTGTCACTGCTCATCGGCACAGCCACAGTATGAATCAGCGCACATAAACTCTTTAGCATCTACATATAACTCTACAGACAGATCTGGATGTACAGCGAACGAGACCGGGAGGAGCACAAAGTCCGCTCAGCTTCGCCTCGTGGGGAGAGATGCGGGATAATAAGCAACTCTAGGGCAGGAAAGTGCCTGCTGACGAATTGGTCTGGCATGGATTCCACACTATAGGATAGTACAGGGTATATTGAGTGCCCCACTGAACCTGTTGGCGGGGTTAGGGATAACAGGGTAATAACAGAATCCCCTTCGGTGTA,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000476.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000476.png,,,,,12f622ea,False
2224,BBF10K_000477,OYC-bridge-AGAC-GCAA,OYC-bridge-AGAC-GCAA,Open Yeast Collection,pOpen-OYC-bridge-AGAC-GCAA,pBuild,M13 For,M13 Rev,,,,,,,,,"Bridge for AGAC/GCAA, See design: https://docs.google.com/spreadsheets/d/1hhiKwaTJyWajH1fEUxZ_79DP4TRtlCBLvO6EtcqtxeY/edit?usp=sharing","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,bridge,AGAC,GCAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAAGACAGATCCATCCACCTAGGGTTCACTCAGTTCCTGTATCTAACCGCATTATGTGACGATCAGTTTGTAGATCTGGATGTACAGGCAACGAGACCGAGTGTCGTGTATATGCTGCTATTTACGAGTTCTTCTTCTATTGCATTTGCCTGTGATCTCTATCATTTTAGTGTCTGTTACATCTTTCTTCACAACACCAGCGTGAGTGACTAGCCCTGAGTCCAGATGCCTAACTGTATGCGAGTCATCTTGTATGGAACGATGTCCTGATGTCAGCTGCTCAGCCTCAATCTCTACTGATGTCTACATAAGCGATCCCAAGGTCATACGACTGACCCAATGTAGCTAGGGATAACAGGGTAATCATACCAACTTTTCCAGTCCC,2411,AAGAATTACTGACCCCTCGGGGTCTCAAGACAGATCCATCCACCTAGGGTTCACTCAGTTCCTGTATCTAACCGCATTATGTGACGATCAGTTTGTAGATCTGGATGTACAGGCAACGAGACCGAGTGTCGTGTATATGCTGCTATTTACGAGTTCTTCTTCTATTGCATTTGCCTGTGATCTCTATCATTTTAGTGTCTGTTACATCTTTCTTCACAACACCAGCGTGAGTGACTAGCCCTGAGTCCAGATGCCTAACTGTATGCGAGTCATCTTGTATGGAACGATGTCCTGATGTCAGCTGCTCAGCCTCAATCTCTACTGATGTCTACATAAGCGATCCCAAGGTCATACGACTGACCCAATGTAGCTAGGGATAACAGGGTAATCATACCAACTTTTCCAGTCCC,410,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000477.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000477.png,,,,,fdaea13c,False
2226,BBF10K_000479,OYC-CamR,OYC-resistance-CamR,Open Yeast Collection,pOpen-OYC-CamR,pBuild,M13 For,M13 Rev,,,,,,,,,E coli Resistance Marker,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,GCAA,ACTA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAGCAACATGGTCATAGCTGTTTCCTGACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCGTCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAAACTATGAGACCACCACAGGTCAAGATTCACGGATTGGTACGGCGACCAATCCTAGGGATAACAGGGTAATCAGCGCGAGCTCGACACTCAC,3018,AAGAATTACTGACCCCTCGGGGTCTCAGCAACATGGTCATAGCTGTTTCCTGACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCGTCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAAACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAAACTATGAGACCACCACAGGTCAAGATTCACGGATTGGTACGGCGACCAATCCTAGGGATAACAGGGTAATCAGCGCGAGCTCGACACTCAC,1017,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000479.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000479.png,,,,,aa60847c,False
2227,BBF10K_000480,OYC-SpecR,OYC-resistance-SpecR,Open Yeast Collection,pOpen-OYC-SpecR,pBuild,M13 For,M13 Rev,,,,,,,,,E coli Resistance Marker,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,marker,GCAA,ACTA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAGCAACATGGTCATAGCTGTTTCCTGCAATTGATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACCTAGGTTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCGTCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCCGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATAATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGTAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGACTATGAGACCACCCGTATCGCATAAGGATGTGCAAGAGGCGATTTGGTACCTAGGGATAACAGGGTAATTGTAGGGCGCATTTAGCTTAG,3336,AAGAATTACTGACCCCTCGGGGTCTCAGCAACATGGTCATAGCTGTTTCCTGCAATTGATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACCTAGGTTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCGTCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCCGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATAATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGTAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGACTATGAGACCACCCGTATCGCATAAGGATGTGCAAGAGGCGATTTGGTACCTAGGGATAACAGGGTAATTGTAGGGCGCATTTAGCTTAG,1335,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000480.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000480.png,,,,,628cae46,False
2228,BBF10K_000481,OYC-high-copy-origin,OYC-high-copy-origin,Open Yeast Collection,pOpen-OYC-high-copy-origin,pBuild,M13 For,M13 Rev,,,,,,,,,E coli high copy origin,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,origin,ACTA,AAAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCAACTATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAACGCCAGGGTTTTCCCAGTCACGACAAAATGAGACCACGAGATGATGCACCGATAGCTTCGGCTGAGATACGATAATTAGGGATAACAGGGTAATATAGCTACACTGATCTAGGCT,2736,AAGAATTACTGACCCCTCGGGGTCTCAACTATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAACGCCAGGGTTTTCCCAGTCACGACAAAATGAGACCACGAGATGATGCACCGATAGCTTCGGCTGAGATACGATAATTAGGGATAACAGGGTAATATAGCTACACTGATCTAGGCT,735,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000481.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000481.png,,,,,3db84468,False
2229,BBF10K_000482,OYC-eforRed-dropout,OYC-eforRed-dropout-cassette,Open Yeast Collection,pOpen-OYC-eforRed-cassette,pBuild,M13 For,M13 Rev,,,,,,,,,"eforRed selection cassette for level 1 vector, clones into backbone and used for colony selection following Level 1 assembly","Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,reporter cassette,GCAA,AAAA,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGCTCGAGGCAACGAGACCGGAGTAGTCTTCGAAAGTGAAACGTGATTTCATGCGTCATTTTGAACATTTTGTAAATCTTATTTAATAATGTGTGCGGCAATTCACATTTAATTTATGAATGTTTTCTTAACATCGCGGCAACTCAAGAAACGGCAGGTTCGGATCTTAGCTACTAGAGAAAGAGGAGAAATACTAGCATATGTCCGTGATTAAACAGGTGATGAAAACCAAACTGCACTTGGAAGGCACCGTGAACGGTCACGATTTCACCATCGAGGGTAAAGGTGAAGGTAAACCGTATGAAGGTCTGCAACACATGAAAATGACTGTAACTAAAGGTGCCCCGCTCCCGTTTTCCGTCCACATCCTGACTCCGTCCCACATGTACGGTAGCAAGCCGTTCAATAAATATCCAGCGGATATCCCGGACTATCACAAGCAGTCTTTCCCGGAAGGCATGAGCTGGGAACGTTCTATGATCTTCGAAGATGGCGGTGTGTGCACCGCTTCCAACCACTCTTCTATCAACCTCCAGGAAAATTGTTTTATCTACGATGTGAAATTTCATGGTGTTAACCTGCCGCCGGATGGCCCGGTAATGCAGAAAACCATTGCAGGTTGGGAACCGAGCGTGGAAACGCTGTACGTGCGTGACGGTATGCTGAAATCTGACACTGCAATGGTATTCAAACTTAAGGGGGGTGGTCACCACCGTGTTGATTTCAAGACTACCTACAAAGCTAAAAAACCGGTAAAACTGCCGGAATTTCACTTCGTTGAACACCGTTTGGAACTGACCAAGCACGATAAAGACTTCACGACTTGGGACCAGCAAGAAGCTGCGGAAGGCCATTTCTCCCCTCTGCCGAAAGCACTGCCATGAAAGCTTCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATAGAAGACCACGCTGGTCTCTAAAACAATTGACGATGGGGACATAGAACACTCATATCTTCAACCTAAGGCGTAGGGATAACAGGGTAATGCTAACGGGAACTCGTCCCTT,3147,AAGAATTACTGACCCCTCGGCTCGAGGCAACGAGACCGGAGTAGTCTTCGAAAGTGAAACGTGATTTCATGCGTCATTTTGAACATTTTGTAAATCTTATTTAATAATGTGTGCGGCAATTCACATTTAATTTATGAATGTTTTCTTAACATCGCGGCAACTCAAGAAACGGCAGGTTCGGATCTTAGCTACTAGAGAAAGAGGAGAAATACTAGCATATGTCCGTGATTAAACAGGTGATGAAAACCAAACTGCACTTGGAAGGCACCGTGAACGGTCACGATTTCACCATCGAGGGTAAAGGTGAAGGTAAACCGTATGAAGGTCTGCAACACATGAAAATGACTGTAACTAAAGGTGCCCCGCTCCCGTTTTCCGTCCACATCCTGACTCCGTCCCACATGTACGGTAGCAAGCCGTTCAATAAATATCCAGCGGATATCCCGGACTATCACAAGCAGTCTTTCCCGGAAGGCATGAGCTGGGAACGTTCTATGATCTTCGAAGATGGCGGTGTGTGCACCGCTTCCAACCACTCTTCTATCAACCTCCAGGAAAATTGTTTTATCTACGATGTGAAATTTCATGGTGTTAACCTGCCGCCGGATGGCCCGGTAATGCAGAAAACCATTGCAGGTTGGGAACCGAGCGTGGAAACGCTGTACGTGCGTGACGGTATGCTGAAATCTGACACTGCAATGGTATTCAAACTTAAGGGGGGTGGTCACCACCGTGTTGATTTCAAGACTACCTACAAAGCTAAAAAACCGGTAAAACTGCCGGAATTTCACTTCGTTGAACACCGTTTGGAACTGACCAAGCACGATAAAGACTTCACGACTTGGGACCAGCAAGAAGCTGCGGAAGGCCATTTCTCCCCTCTGCCGAAAGCACTGCCATGAAAGCTTCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATAGAAGACCACGCTGGTCTCTAAAACAATTGACGATGGGGACATAGAACACTCATATCTTCAACCTAAGGCGTAGGGATAACAGGGTAATGCTAACGGGAACTCGTCCCTT,1146,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000482.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000482.png,,,,,17e04ad9,False
2230,BBF10K_000484,pWV01-ori,pWV01-ori,Open Yeast Collection,pOpen-pWV01-ori,pBuild,M13 For,M13 Rev,,,,,,,,,temperature sensitive E. coli origin of replication,"Purified Plasmid DNA, Stained with Cresol Red","37 C, shaking 300 rpm",LB or 2YT,ampicillin,,,,Golden Gate Assembly,BsaI,origin,,,CGCTGAGGTGTCAATCGTCGGAGCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCATGGTCATAGCTGTTTCCTGAGAGCTTGGCAGGTGATGACACACATTAACAAATTTCGTGAGGAGTCTCCAGAAGAATGCCATTAATTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGGCCTACTATTAGCAACAACGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGAACCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACCTGCACCAGTCAGTAAAACGACGGCCAGTAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTTGGTTCAGGTGGAGTGGGAGAAGAATTACTGACCCCTCGGGGTCTCCACTATTTTTATTAAAATTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGCCCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAAATAATAAGCGCAGCGCCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTATGAGGGAATGAAATTCCCTCATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAATTTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTGCCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTAAGGGGTTTTTCAATCGCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTGTTGTTTTTATGATTACAAAGTGATACACTAACTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAGAATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACAAAAAGGTTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAAAAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATTATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAATGATTGGAAAGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAAAAAAAAGATAAAGATACATGGAATAATAGTAATATTATACAAAATGGAAAGCACTATAAAAAACCACACTATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATTGGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCATGAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATTTTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTTTTAGATATAGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTTGGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTGAGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAAATGACAAACAAAGAAAAAGAGTTATTTGCTGAAAATGAGGAATTAAAAAAAGAAATTAAGGACTTAAAAGAGCGTATTGAAAGATACAGAGAAATGGAAGTTGAATTAAGTACAACAATAGATTTATTGAGAGGAGGGATTATTGAATAAATAAAAGCCCCCTGACGAAAGTCGAAGGGGGTTTTTATTTTGGTTTGATGTTGCGATTAATAGCAATACAATTGCAATAAACAACGCCAGGGTTTTCCCAGTCACGACAAAAGGAGACCAACGGAGCCCTTATTGTAACCCCCCATAACCCACTGTCGTTATAGGGATAACAGGGTAATATAGTATCTTCAGGCCGATGA,3861,AAGAATTACTGACCCCTCGGGGTCTCCACTATTTTTATTAAAATTTTAGCGTTTATTTCGTTTAGTTATCGGCATAATCGTTAAAACAGGCGTTATCGTAGCGTAAAAGCCCTTGAGCGTAGCGTGGCTTTGCAGCGAAGATGTTGTCTGTTAGATTATGAAAGCCGATGACTGAATGAAATAATAAGCGCAGCGCCCTTCTATTTCGGTTGGAGGAGGCTCAAGGGAGTATGAGGGAATGAAATTCCCTCATGGGTTTGATTTTAAAAATTGCTTGCAATTTTGCCGAGCGGTAGCGCTGGAAAATTTTTGAAAAAAATTTGGAATTTGGAAAAAAATGGGGGGAAAGGAAGCGAATTTTGCTTCCGTACTACGACCCCCCATTAAGTGCCGAGTGCCAATTTTTGTGCCAAAAACGCTCTATCCCAACTGGCTCAAGGGTTTAAGGGGTTTTTCAATCGCCAACGAATCGCCAACGTTTTCGCCAACGTTTTTTATAAATCTATATTTAAGTAGCTTTATTGTTGTTTTTATGATTACAAAGTGATACACTAACTTTATAAAATTATTTGATTGGAGTTTTTTAAATGGTGATTTCAGAATCGAAAAAAAGAGTTATGATTTCTCTGACAAAAGAGCAAGATAAAAAATTAACAGATATGGCGAAACAAAAAGGTTTTTCAAAATCTGCGGTTGCGGCGTTAGCTATAGAAGAATATGCAAGAAAGGAATCAGAACAAAAAAAATAAGCGAAAGCTCGCGTTTTTAGAAGGATACGAGTTTTCGCTACTTGTTTTTGATAAGGTAATTATATCATGGCTATTAAAAATACTAAAGCTAGAAATTTTGGATTTTTATTATATCCTGACTCAATTCCTAATGATTGGAAAGAAAAATTAGAGAGTTTGGGCGTATCTATGGCTGTCAGTCCTTTACACGATATGGACGAAAAAAAAGATAAAGATACATGGAATAATAGTAATATTATACAAAATGGAAAGCACTATAAAAAACCACACTATCACGTTATATATATTGCACGAAATCCTGTAACAATAGAAAGCGTTAGGAACAAGATTAAGCGAAAATTGGGGAATAGTTCAGTTGCTCATGTTGAGATACTTGATTATATCAAAGGTTCATATGAATATTTGACTCATGAATCAAAGGACGCTATTGCTAAGAATAAACATATATACGACAAAAAAGATATTTTGAACATTAATGATTTTGATATTGACCGCTATATAACACTTGATGAAAGCCAAAAAAGAGAATTGAAGAATTTACTTTTAGATATAGTGGATGACTATAATTTGGTAAATACAAAAGATTTAATGGCTTTTATTCGCCTTAGGGGAGCGGAGTTTGGAATTTTAAATACGAATGATGTAAAAGATATTGTTTCAACAAACTCTAGCGCCTTTAGATTATGGTTTGAGGGCAATTATCAGTGTGGATATAGAGCAAGTTATGCAAAGGTTCTTGATGCTGAAACGGGGGAAATAAAATGACAAACAAAGAAAAAGAGTTATTTGCTGAAAATGAGGAATTAAAAAAAGAAATTAAGGACTTAAAAGAGCGTATTGAAAGATACAGAGAAATGGAAGTTGAATTAAGTACAACAATAGATTTATTGAGAGGAGGGATTATTGAATAAATAAAAGCCCCCTGACGAAAGTCGAAGGGGGTTTTTATTTTGGTTTGATGTTGCGATTAATAGCAATACAATTGCAATAAACAACGCCAGGGTTTTCCCAGTCACGACAAAAGGAGACCAACGGAGCCCTTATTGTAACCCCCCATAACCCACTGTCGTTATAGGGATAACAGGGTAATATAGTATCTTCAGGCCGATGA,1860,ColE1 High Copy,500-700,BSL1,,,https://freegenes.github.io/genbank/BBF10K_000484.gb,Scott Pownall,,,we are unaware,freegenes.github.io/genes/images/BBF10K_000484.png,,,,,6e5f9794,False