{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# 3.0 Ligand-Receptor Sym-vs-Asym Differential Regulation"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      ">> clustergrammer2 backend version 0.5.1\n"
     ]
    }
   ],
   "source": [
    "from clustergrammer2 import net\n",
    "df = {}\n",
    "from glob import glob"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {},
   "outputs": [],
   "source": [
    "all_files = glob('../data/lig-rec_*')\n",
    "\n",
    "for inst_file in all_files:\n",
    "    inst_comp = inst_file.split('_')[-1].replace('.txt','')\n",
    "    net.load_file(inst_file)\n",
    "    df[inst_comp] = net.export_df()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD4-CD4"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "18c72c2d81a04ce7a41076ad11b0f786",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 39, \"clust\": 30, \"rank\": 1, \"rankvar\": 35, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD4-CD4'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD4-CD8"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "da52c893a1cc45eca962c573b74b0651",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 43, \"clust\": 41, \"rank\": 23, \"rankvar\": 3, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD4-CD8'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD4-Mac"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "a36007ccc7404699b5002c6c694b6521",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 57, \"clust\": 20, \"rank\": 22, \"rankvar\": 19, \"g…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD4-Mac'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD8-CD4"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 6,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "b28f9e94e31845069a5fea5f5695584e",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 43, \"clust\": 31, \"rank\": 3, \"rankvar\": 38, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD8-CD4'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD8-CD8"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "d8a332363ba34c10a3015325afe0d118",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"TGFB1\", \"ini\": 48, \"clust\": 29, \"rank\": 16, \"rankvar\": 26, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD8-CD8'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: CD8-Mac"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "57b1d642493040f88ac3cd25ef74814b",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 55, \"clust\": 16, \"rank\": 29, \"rankvar\": 4, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['CD8-Mac'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: Mac-CD4"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 9,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "7a4293874d4247efba0312c0252263d6",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 60, \"clust\": 58, \"rank\": 6, \"rankvar\": 51, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['Mac-CD4'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: Mac-CD8"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 10,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "ab1077e2cd4c45fc9d40b8ca68e06108",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"SEMA4A\", \"ini\": 60, \"clust\": 30, \"rank\": 38, \"rankvar\": 1, \"gr…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['Mac-CD8'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# Ligand Receptor Sym/Asym Fold Change: Mac-Mac"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "application/vnd.jupyter.widget-view+json": {
       "model_id": "1808268a03764a48a736b9e70f8d8d0c",
       "version_major": 2,
       "version_minor": 0
      },
      "text/plain": [
       "ExampleWidget(network='{\"row_nodes\": [{\"name\": \"NUCB2\", \"ini\": 60, \"clust\": 58, \"rank\": 32, \"rankvar\": 1, \"gro…"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "net.load_df(df['Mac-Mac'])\n",
    "net.widget()"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
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  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": []
  }
 ],
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13.0, 10.0, 10.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"TNFSF13B\", \"ini\": 47, \"clust\": 54, \"rank\": 41, \"rankvar\": 33, \"group\": [47.0, 40.0, 36.0, 30.0, 26.0, 21.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"BGN\", \"ini\": 46, \"clust\": 53, \"rank\": 15, \"rankvar\": 45, \"group\": [48.0, 41.0, 37.0, 31.0, 27.0, 22.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"ADAM15\", \"ini\": 45, \"clust\": 17, \"rank\": 25, \"rankvar\": 31, \"group\": [12.0, 11.0, 11.0, 9.0, 9.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"LPL\", \"ini\": 44, \"clust\": 33, \"rank\": 10, \"rankvar\": 51, \"group\": [29.0, 23.0, 19.0, 15.0, 13.0, 10.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"CXCL10\", \"ini\": 43, \"clust\": 21, \"rank\": 30, \"rankvar\": 19, \"group\": [20.0, 17.0, 15.0, 12.0, 11.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"OSM\", \"ini\": 42, \"clust\": 52, \"rank\": 26, \"rankvar\": 38, \"group\": [49.0, 42.0, 38.0, 32.0, 28.0, 23.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"CXCL9\", \"ini\": 41, \"clust\": 22, \"rank\": 57, \"rankvar\": 41, \"group\": [21.0, 17.0, 15.0, 12.0, 11.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"ANXA1\", \"ini\": 40, \"clust\": 40, \"rank\": 36, \"rankvar\": 25, \"group\": [37.0, 31.0, 27.0, 21.0, 18.0, 14.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"COL18A1\", \"ini\": 39, \"clust\": 16, \"rank\": 24, \"rankvar\": 43, \"group\": [14.0, 11.0, 11.0, 9.0, 9.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"ADAM28\", \"ini\": 38, \"clust\": 45, \"rank\": 39, \"rankvar\": 7, \"group\": [39.0, 32.0, 28.0, 22.0, 19.0, 15.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"LRPAP1\", \"ini\": 37, \"clust\": 27, \"rank\": 28, \"rankvar\": 36, \"group\": [32.0, 26.0, 22.0, 17.0, 14.0, 10.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"TNFSF10\", \"ini\": 36, \"clust\": 51, \"rank\": 31, \"rankvar\": 0, \"group\": [50.0, 43.0, 39.0, 33.0, 29.0, 24.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"VWF\", \"ini\": 35, \"clust\": 50, \"rank\": 33, \"rankvar\": 2, \"group\": [51.0, 44.0, 40.0, 34.0, 30.0, 25.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"TFPI\", \"ini\": 34, \"clust\": 24, \"rank\": 14, \"rankvar\": 47, \"group\": [22.0, 18.0, 16.0, 13.0, 12.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"HGF\", \"ini\": 33, \"clust\": 49, \"rank\": 40, \"rankvar\": 8, \"group\": [52.0, 45.0, 41.0, 35.0, 31.0, 26.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"COL1A2\", \"ini\": 32, \"clust\": 11, \"rank\": 5, \"rankvar\": 55, \"group\": [11.0, 10.0, 10.0, 8.0, 8.0, 8.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"TNC\", \"ini\": 31, \"clust\": 14, \"rank\": 2, \"rankvar\": 59, \"group\": [16.0, 13.0, 11.0, 9.0, 9.0, 9.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"CTGF\", \"ini\": 30, \"clust\": 29, \"rank\": 12, \"rankvar\": 50, \"group\": [27.0, 21.0, 18.0, 14.0, 13.0, 10.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, {\"name\": \"ICAM1\", \"ini\": 29, \"clust\": 28, \"rank\": 23, \"rankvar\": 27, \"group\": [28.0, 22.0, 18.0, 14.0, 13.0, 10.0, 1.0, 1.0, 1.0, 1.0, 1.0]}, 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       "value": "Hello World!"
      }
     },
     "18c72c2d81a04ce7a41076ad11b0f786": {
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      "model_module_version": "^0.5.1",
      "model_name": "ExampleModel",
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       "_view_module_version": "^0.5.1",
       "layout": "IPY_MODEL_13e220ec3a08401f90d9929f7fc525c5",
       "mat_string": "ini_mat_string",
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       "value": "row-label -> ICAM3"
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       "value": "row-label -> CCL5"
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