{ "cells": [ { "cell_type": "markdown", "metadata": {}, "source": [ "PyImageJ Tutorial\n", "===\n", "\n", "This notebook covers how to use ImageJ as a library from Python. A major advantage of this approach is the ability to combine ImageJ with other tools available from the Python software ecosystem, including NumPy, SciPy, scikit-image, CellProfiler, OpenCV, ITK and more.\n", "\n", "This notebook assumes familiarity with the ImageJ API. Detailed tutorials in that regard can be found in the other notebooks." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "## 5 Convenience methods of PyImageJ\n", "\n", "PyImageJ is built to provide easy access to key ImageJ resources. We call these collective methods \"conveience methods\". These methods are attached to`ij.py` after initializing ImageJ. Here's a quick list of some of the more useful methods and additional information." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "| `ij.py.` | function | more information |\n", "| :---: | :---: | :---: |\n", "| `show` | Show an image | [2-Opening-and-Displaying-Images](../doc/2-Opening-and-Displaying-Images.ipynb)\n", "| `to_java` | Convert data from Python to Java | [3-Sending-Data-to-Java](../doc/3-Sending-Data-to-Java.ipynb) |\n", "| `from_java` | Convert data from Java to Python | [4-Retrieving-Data-from-Java](../doc/4-Retrieving-Data-from-Java.ipynb) |\n", "| `run_macro` | Run an original ImageJ macro | [7-Running-Macros-Scripts-and-Plugins](../doc/7-Running-Macros-Scripts-and-Plugins.ipynb) |\n", "| `run_script` | Run an ImageJ script (supported languages) | [7-Running-Macros-Scripts-and-Plugins](../doc/7-Running-Macros-Scripts-and-Plugins.ipynb) |\n", "| `run_plugin` | Run a plugin | [7-Running-Macros-Scripts-and-Plugins](../doc/7-Running-Macros-Scripts-and-Plugins.ipynb) |\n", "| `initialize_numpy_image` | Create a new numpy image in the same shape as input image | [6-Working-with-Images](../doc/6-Working-with-Images.ipynb) |\n", "| `sync_image` | Syncronize data between ImageJ and ImageJ2 data structures | -- |\n", "| `active_image_plus` | Get the `ImagePlus` from the current window | -- |\n", "| `active_xarray` | Convert current window into an `xarray.DataArray` | -- |\n", "| `active_dataset` | Get the active `Dataset` from the Dataset service | -- |" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "There are other convenience methods that are attached to `ij.py`. After initializing ImageJ you can explore `ij.py`'s methods with `dir`." ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "### 5.1 Other convenient access to ImageJ functions\n", "\n", "When the original ImageJ is available (_i.e._ the legacy layer is active) `IJ`, `WindowManager`, `ResultsTable` and `RoiManager` are accessible directly from the initialized `ij` object." ] }, { "cell_type": "code", "execution_count": 1, "metadata": {}, "outputs": [ { "name": "stderr", "output_type": "stream", "text": [ "log4j:WARN No appenders could be found for logger (org.bushe.swing.event.EventService).\n", "log4j:WARN Please initialize the log4j system properly.\n", "log4j:WARN See http://logging.apache.org/log4j/1.2/faq.html#noconfig for more info.\n", "WARNING:root:Operating in headless mode - the original ImageJ will have limited functionality.\n" ] }, { "name": "stdout", "output_type": "stream", "text": [ "ImageJ version: 2.3.0/1.53f\n", "Legacy layer active: True\n" ] } ], "source": [ "import imagej\n", "\n", "# initialize imagej\n", "ij = imagej.init()\n", "print(f\"ImageJ version: {ij.getVersion()}\")\n", "\n", "# first check if the legacy layer is active\n", "print(f\"Legacy layer active: {ij.legacy.isActive()}\")" ] }, { "cell_type": "code", "execution_count": 2, "metadata": {}, "outputs": [ { "name": "stderr", "output_type": "stream", "text": [ "WARNING:root:Operating in headless mode - the IJ class will not be fully functional.\n", "WARNING:root:Operating in headless mode - the ResultsTable class will not be fully functional.\n", "WARNING:root:Operating in headless mode - the RoiManager class will not be fully functional.\n", "WARNING:root:Operating in headless mode - the WindowManager class will not be fully functional.\n" ] }, { "name": "stdout", "output_type": "stream", "text": [ "<java class 'ij.IJ'>\n", "<java class 'ij.measure.ResultsTable'>\n", "<java class 'ij.plugin.frame.RoiManager'>\n", "<java class 'ij.WindowManager'>\n" ] } ], "source": [ "# demonstrate access to classes\n", "print(ij.IJ)\n", "print(ij.ResultsTable)\n", "print(ij.RoiManager)\n", "print(ij.WindowManager)" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "Note the warnings! We're currently in headless mode. The many legacy ImageJ functions operate limitedly or not at all in headless mode. For example the `RoiManager` is not functional in a true headless enviornment." ] } ], "metadata": { "interpreter": { "hash": "ffb7cfab4b775ee54ab00d6ea209daa40cc054b7bb58fbad3b74bb1492b78b3c" }, "kernelspec": { "display_name": "Python 3.8.10 ('pyijdev')", "language": "python", "name": "python3" }, "language_info": { "codemirror_mode": { "name": "ipython", "version": 3 }, "file_extension": ".py", "mimetype": "text/x-python", "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", "version": "3.8.10" }, "orig_nbformat": 4 }, "nbformat": 4, "nbformat_minor": 2 }