LOCUS pUCwInsert 7564 bp circular 07-SEP-2015 UNIMARK pUCwInsert annotations pUCwInsert FEATURES Location/Qualifiers misc_feature 1..7564 /comment="Molecule is created with Unipro UGENE v1.17" /ugene_name="source" -10_signal complement(114..119) /note="Plac promoter (drives lacZalpha; counter-clockwise); TATGTT" -35_signal complement(138..143) /note="Plac promoter (drives lacZalpha; counter-clockwise); TTTACA" protein_bind complement(163..175) /note="CAP protein binding site" /bound_moiety="catabolite activator protein (CAP)" rep_origin complement(467..1055) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" -35_signal 873..878 /note="RNAI promoter (clockwise); TTGAAG" -10_signal 895..900 /note="RNAI promoter (clockwise); GCTACA" -10_signal complement(1029..1034) /note="RNAII promoter (counter-clockwise); CGTAAT" -35_signal complement(1050..1055) /note="RNAII promoter (counter-clockwise); TTGAGA" gene complement(1226..2086) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(2018..2086) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." -10_signal complement(2130..2135) /note="bla promoter (counter-clockwise); GAGACA" -35_signal complement(2151..2156) /note="bla promoter (counter-clockwise); TTCAAA" variation 3193..3193 /note="b2 substitution terminating at the att site" CDS 3429..4013 /codon_start=1 /transl_table=11 /product="orf-194" /protein_id="AAA96558.1" /db_xref="GI:215129" /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENFTWLSP GGEYQKWNGTAWVKDTEAEKLFRIREAEETKKSLMQVASEHIAPLQDAADLEIATKEE TSLLEAWKKYRVLLNRVDTSTAPDIEWPAVPVME" CDS complement(4142..5374) /codon_start=1 /transl_table=11 /product="orf206b" /protein_id="AAA96559.1" /db_xref="GI:508992" /translation="MTKKPWERRLKDLSHLLKCCIDTYFDPELFRLNLNQFLQTARTV TFIIQKNKNQIIGYDIWYNNNVIEKWKNDPLMAWAKNSRNTIEKQGDLEMYSEAKATL ISSYIEENDIEFITNESMLNIGIKKLVRLAQKKLPSYLTESSIIKSERRWVANTLKDY ELLHALAIIYGRMYNCCNSLGIQINNPMGDDVISPTSFDSLFDEARRITYLKLKDYSI SKLSFSMIQYDNKIIPEDIKERLKLVDKPKNITSTEELVDYTAKLAETTFLKDGYHIQ TLIFYDKQFHPIDLINTTFEDQADKYIFWRYAADRAKITNAYGFIWISELWLRKASIY SNKPIHTMPIIDERLQVIGIDSNNNQKCISWKIVRENEEKKPTLEISTADSKHDEKPY FMRSVLKAIGGDVNTMNN" mRNA complement(4687..4687) /note="mRNA-pbl" misc_recomb 5845..5846 CDS complement(5965..6855) /codon_start=1 /transl_table=11 /product="orf206b" /protein_id="AAA96560.1" /db_xref="GI:215131" /translation="MKKLPLPARTYSEMLNKCSEGMMQINVRNNFITHFPTFLQKEQQ YRILSSTGQLFTYDRTHPLEPTTLVVGNLTKVKLEKLYENNLRDKNKPARTYYDDMLV SSGEKCPFCGDIGQTKNIDHFLPIAHYPEFSVMPINLVPSCRDCNMGEKGQVFAVDEV HQAIHPYIDKDIFFREQWVYANFVSGTPGAISFYVECPANWRQEDKHRALHHFKLLNI ANRYRLEAGKHLSEVITQRNSFVKVIRKYSSTATFQQLQSEFIEANLKPIIDLNDFPN YWKRVMYQCLANSEDFFRGI" ORIGIN 1 CGAGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCGTAAT 61 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 121 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 181 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 241 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 301 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 361 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 421 GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC 481 GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG 541 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 601 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC 661 ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG 721 TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT 781 CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA 841 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 901 CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG 961 TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA 1021 AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG 1081 GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA 1141 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA 1201 TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG 1261 CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA 1321 TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC 1381 CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC 1441 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA 1501 GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC 1561 GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT 1621 GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA 1681 GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG 1741 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG 1801 AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC 1861 CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT 1921 CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT 1981 CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG 2041 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC 2101 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 2161 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 2221 TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT 2281 TTCGTCTCGC GCGTTTCGGT GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA 2341 CGGTCACAGC TTGTCTGTAA GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG 2401 CGGGTGTTGG CGGGTGTCGG GGCTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA 2461 GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA 2521 GGCGCCATTC GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT 2581 CGCTATTACG CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC 2641 CAGGGTTTTC CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTCGGC CTTTCCGGCA 2701 GGTGCGCCGA TCCCGTGGCC ATCAGATATC GTTCCGTCTG GCTACGTCCT GATGCAGGGG 2761 CAGGCGTTTG ACAAATCAGC CTACCCAAAA CTTGCTGTCG CGTATCCATC GGGTGTGCTT 2821 CCTGATATGC GAGGCTGGAC AATCAAGGGG AAACCCGCCA GCGGTCGTGC TGTATTGTCT 2881 CAGGAACAGG ATGGAATTAA GTCGCACACC CACAGTGCCA GTGCATCCGG TACGGATTTG 2941 GGGACGAAAA CCACATCGTC GTTTGATTAC GGGACGAAAA CAACAGGCAG TTTCGATTAC 3001 GGCACCAAAT CGACGAATAA CACGGGGGCT CATGCTCACA GTCTGAGCGG TTCAACAGGG 3061 GCCGCGGGTG CTCATGCCCA CACAAGTGGT TTAAGGATGA ACAGTTCTGG CTGGAGTCAG 3121 TATGGAACAG CAACCATTAC AGGAAGTTTA TCCACAGTTA AAGGAACCAG CACACAGGGT 3181 ATTGCTTATT TATCGAAAAC GGACAGTCAG GGCAGCCACA GTCACTCATT GTCCGGTACA 3241 GCCGTGAGTG CCGGTGCACA TGCGCATACA GTTGGTATTG GTGCGCACCA GCATCCGGTT 3301 GTTATCGGTG CTCATGCCCA TTCTTTCAGT ATTGGTTCAC ACGGACACAC CATCACCGTT 3361 AACGCTGCGG GTAACGCGGA AAACACCGTC AAAAACATTG CATTTAACTA TATTGTGAGG 3421 CTTGCATAAT GGCATTCAGA ATGAGTGAAC AACCACGGAC CATAAAAATT TATAATCTGC 3481 TGGCCGGAAC TAATGAATTT ATTGGTGAAG GTGACGCATA TATTCCGCCT CATACCGGTC 3541 TGCCTGCAAA CAGTACCGAT ATTGCACCGC CAGATATTCC GGCTGGCTTT GTGGCTGTTT 3601 TCAACAGTGA TGAGGCATCG TGGCATCTCG TTGAAGACCA TCGGGGTAAA ACCGTCTATG 3661 ACGTGGCTTC CGGCGACGCG TTATTTATTT CTGAACTCGG TCCGTTACCG GAAAATTTTA 3721 CCTGGTTATC GCCGGGAGGG GAATATCAGA AGTGGAACGG CACAGCCTGG GTGAAGGATA 3781 CGGAAGCAGA AAAACTGTTC CGGATCCGGG AGGCGGAAGA AACAAAAAAA AGCCTGATGC 3841 AGGTAGCCAG TGAGCATATT GCGCCGCTTC AGGATGCTGC AGATCTGGAA ATTGCAACGA 3901 AGGAAGAAAC CTCGTTGCTG GAAGCCTGGA AGAAGTATCG GGTGTTGCTG AACCGTGTTG 3961 ATACATCAAC TGCACCTGAT ATTGAGTGGC CTGCTGTCCC TGTTATGGAG TAATCGTTTT 4021 GTGATATGCC GCAGAAACGT TGTATGAAAT AACGTTCTGC GGTTAGTTAG TATATTGTAA 4081 AGCTGAGTAT TGGTTTATTT GGCGATTATT ATCTTCAGGA GAATAATGGA AGTTCTATGA 4141 CTCAATTGTT CATAGTGTTT ACATCACCGC CAATTGCTTT TAAGACTGAA CGCATGAAAT 4201 ATGGTTTTTC GTCATGTTTT GAGTCTGCTG TTGATATTTC TAAAGTCGGT TTTTTTTCTT 4261 CGTTTTCTCT AACTATTTTC CATGAAATAC ATTTTTGATT ATTATTTGAA TCAATTCCAA 4321 TTACCTGAAG TCTTTCATCT ATAATTGGCA TTGTATGTAT TGGTTTATTG GAGTAGATGC 4381 TTGCTTTTCT GAGCCATAGC TCTGATATCC AAATGAAGCC ATAGGCATTT GTTATTTTGG 4441 CTCTGTCAGC TGCATAACGC CAAAAAATAT ATTTATCTGC TTGATCTTCA AATGTTGTAT 4501 TGATTAAATC AATTGGATGG AATTGTTTAT CATAAAAAAT TAATGTTTGA ATGTGATAAC 4561 CGTCCTTTAA AAAAGTCGTT TCTGCAAGCT TGGCTGTATA GTCAACTAAC TCTTCTGTCG 4621 AAGTGATATT TTTAGGCTTA TCTACCAGTT TTAGACGCTC TTTAATATCT TCAGGAATTA 4681 TTTTATTGTC ATATTGTATC ATGCTAAATG ACAATTTGCT TATGGAGTAA TCTTTTAATT 4741 TTAAATAAGT TATTCTCCTG GCTTCATCAA ATAAAGAGTC GAATGATGTT GGCGAAATCA 4801 CATCGTCACC CATTGGATTG TTTATTTGTA TGCCAAGAGA GTTACAGCAG TTATACATTC 4861 TGCCATAGAT TATAGCTAAG GCATGTAATA ATTCGTAATC TTTTAGCGTA TTAGCGACCC 4921 ATCGTCTTTC TGATTTAATA ATAGATGATT CAGTTAAATA TGAAGGTAAT TTCTTTTGTG 4981 CAAGTCTGAC TAACTTTTTT ATACCAATGT TTAACATACT TTCATTTGTA ATAAACTCAA 5041 TGTCATTTTC TTCAATGTAA GATGAAATAA GAGTAGCCTT TGCCTCGCTA TACATTTCTA 5101 AATCGCCTTG TTTTTCTATC GTATTGCGAG AATTTTTAGC CCAAGCCATT AATGGATCAT 5161 TTTTCCATTT TTCAATAACA TTATTGTTAT ACCAAATGTC ATATCCTATA ATCTGGTTTT 5221 TGTTTTTTTG AATAATAAAT GTTACTGTTC TTGCGGTTTG GAGGAATTGA TTCAAATTCA 5281 AGCGAAATAA TTCAGGGTCA AAATATGTAT CAATGCAGCA TTTGAGCAAG TGCGATAAAT 5341 CTTTAAGTCT TCTTTCCCAT GGTTTTTTAG TCATAAAACT CTCCATTTTG ATAGGTTGCA 5401 TGCTAGATGC TGATATATTT TAGAGGTGAT AAAATTAACT GCTTAACTGT CAATGTAATA 5461 CAAGTTGTTT GATCTTTGCA ATGATTCTTA TCAGAAACCA TATAGTAAAT TAGTTACACA 5521 GGAAATTTTT AATATTATTA TTATCATTCA TTATGTATTA AAATTAGAGT TGTGGCTTGG 5581 CTCTGCTAAC ACGTTGCTCA TAGGAGATAT GGTAGAGCCG CAGACACGTC GTATGCAGGA 5641 ACGTGCTGCG GCTGGCTGGT GAACTTCCGA TAGTGCGGGT GTTGAATGAT TTCCAGTTGC 5701 TACCGATTTT ACATATTTTT TGCATGAGAG AATTTGTACC ACCTCCCACC GACCATCTAT 5761 GACTGTACGC CACTGTCCCT AGGACTGCTA TGTGCCGGAG CGGACATTAC AAACGTCCTT 5821 CTCGGTGCAT GCCACTGTTG CCAATGACCT GCCTAGGAAT TGGTTAGCAA GTTACTACCG 5881 GATTTTGTAA AAACAGCCCT CCTCATATAA AAAGTATTCG TTCACTTCCG ATAAGCGTCG 5941 TAATTTTCTA TCTTTCATCA TATTCTAGAT CCCTCTGAAA AAATCTTCCG AGTTTGCTAG 6001 GCACTGATAC ATAACTCTTT TCCAATAATT GGGGAAGTCA TTCAAATCTA TAATAGGTTT 6061 CAGATTTGCT TCAATAAATT CTGACTGTAG CTGCTGAAAC GTTGCGGTTG AACTATATTT 6121 CCTTATAACT TTTACGAAAG AGTTTCTTTG AGTAATCACT TCACTCAAGT GCTTCCCTGC 6181 CTCCAAACGA TACCTGTTAG CAATATTTAA TAGCTTGAAA TGATGAAGAG CTCTGTGTTT 6241 GTCTTCCTGC CTCCAGTTCG CCGGGCATTC AACATAAAAA CTGATAGCAC CCGGAGTTCC 6301 GGAAACGAAA TTTGCATATA CCCATTGCTC ACGAAAAAAA ATGTCCTTGT CGATATAGGG 6361 ATGAATCGCT TGGTGTACCT CATCTACTGC GAAAACTTGA CCTTTCTCTC CCATATTGCA 6421 GTCGCGGCAC GATGGAACTA AATTAATAGG CATCACCGAA AATTCAGGAT AATGTGCAAT 6481 AGGAAGAAAA TGATCTATAT TTTTTGTCTG TCCTATATCA CCACAAAATG GACATTTTTC 6541 ACCTGATGAA ACAAGCATGT CATCGTAATA TGTTCTAGCG GGTTTGTTTT TATCTCGGAG 6601 ATTATTTTCA TAAAGCTTTT CTAATTTAAC CTTTGTCAGG TTACCAACTA CTAAGGTTGT 6661 AGGCTCAAGA GGGTGTGTCC TGTCGTAGGT AAATAACTGA CCTGTCGAGC TTAATATTCT 6721 ATATTGTTGT TCTTTCTGCA AAAAAGTGGG GAAGTGAGTA ATGAAATTAT TTCTAACATT 6781 TATCTGCATC ATACCTTCCG AGCATTTATT AAGCATTTCG CTATAAGTTC TCGCTGGAAG 6841 AGGTAGTTTT TTCATTGTAC TTTACCTTCA TCTCTGTTCA TTATCATCGC TTTTAAAACG 6901 GTTCGACCTT CTAATCCTAT CTGACCATTA TAATTTTTTA GAATGGTTTC ATAAGAAAGC 6961 TCTGAATCAA CGGACTGCGA TAATAAGTGG TGGTATCCAG AATTTGTCAC TTCAAGTAAA 7021 AACACCTCAC GAGTTAAAAC ACCTAAGTTC TCACCGAATG TCTCAATATC CGGACGGATA 7081 ATATTTATTG CTTCTCTTGA CCGTAGGACT TTCCACATGC AGGATTTTGG AACCTCTTGC 7141 AGTACTACTG GGGAATGAGT TGCAATTATT GCTACACCAT TGCGTGCATC GAGTAAGTCG 7201 CTTAATGTTC GTAAAAAAGC AGAGAGCAAA GGTGGATGCA GATGAACCTC TGGTTCATCG 7261 AATAAAACTA ATGACTTTTC GCCAACGACA TCTACTAATC TTGTGATAGT AAATAAAACA 7321 ATTGCATGTC CAGAGCTCAT TCGAAGCAGA TATTTCTGGA TATTGTCATA AAACAATTTA 7381 GTGAATTTAT CATCGTCCAC TTGAATCTGT GGTTCATTAC GTCTTAACTC TTCATATTTA 7441 GAAATGAGGC TGATGAGTTC CATATTTGAA AAGTTTTCAT CACTACTTAG TTTTTTGATA 7501 GCTTCAAGCC AGAGTTGTCT TTTTCTATCT ACTCTCATAC AACCAATAAA TGCTGAAATG 7561 AATT //