#!/usr/bin/env perl use strict; use warnings; while (<>) { if (/\tXA:Z:(\S+)/) { my $l = $1; print; my @t = split("\t"); while ($l =~ /([^,;]+),([-+]\d+),([^,]+),(\d+);/g) { my $mchr = ($t[6] eq $1)? '=' : $t[6]; # FIXME: TLEN/ISIZE is not calculated! my $seq = $t[9]; my $phred = $t[10]; # if alternative alignment has other orientation than primary, # then print the reverse (complement) of sequence and phred string if ((($t[1]&0x10)>0) xor ($2<0)) { $seq = reverse $seq; $seq =~ tr/ACGTacgt/TGCAtgca/; $phred = reverse $phred; } print(join("\t", $t[0], 0x100|($t[1]&0x6e9)|($2<0?0x10:0), $1, abs($2), 0, $3, @t[6..7], 0, $seq, $phred, "NM:i:$4"), "\n"); } } else { print; } }