Emily Greenfest-Allen Jie Zheng Chris Stoeckert Elisabetta Manduchi has_genetic_background_of strain a has genetic backgroud as strain b is that strain a is derived from strain b and genetic make-up (all its alleles at all loci) of strain a is same to strain b except the mutated gene of interest and a very small amount of other genetic material, generally from one or two other strains. For example, the genetic background of the mutant strains NOD.129S7(B6)-Rag1tm1Mom/J (003729) and NOD.Cg-Rag1tm1Mom Prf1tm1Sdz/SzJ (004848) is NOD. Person: Jie Zheng produces_low_amount Person: Jie Zheng a produces b if some process that occurs_in a has_output b and amount of b is low relative to normal cases, where a and b are material entities. Examples: defective beta ccell line produces less amount of insulin produces_high_amount a produces b if some process that occurs_in a has_output b and amount of b is high relative to generally cases, where a and b are material entities. Person: Jie Zheng not_produce Person: Jie Zheng a produces_no b if some process that occurs_in a and has_no_output b, where a and b are material entities. has contact relates an entity to a person or an organization that can be contacted for information regarding the entity. has external resource relates an entity to the information about it in an external database. has external identifier relates an entity to an identifier assigned by an external resource which denotes the entity. has email address Person: Jie Zheng a instance-level relation between a person or an organization to an email address the person or organization has. executes shortcut relation of a planned process realizes or concretizes some plan specification has experimental factor relates an investigation to an experimental factor which is a study independent variable specified in a study design. An experimental factor can be a material entity (such as treated and untreated specimen), a quality (such as sex of an organism), a process (such as developmental stage of an organism), or an information about a process (such as duration of a treatment), etc. has experimental factor value relates experimental factor (could be anything that a study independent variable specification is about) to its values For example, biological sex can be a study independent variable, potential values can be male or female. value of experimental factor relates experimental factor values to its factor type (could be anything that a study independent variable specification is about) For example, male/female could be values for biological sex, 0, 0.01, 0.02 mg/L for glucose concentation, 1,5,20 minutes for treatment duration, etc. examines relates an invesitigation to any biologically-related entity that is examined as part of that process, such as a protein, a biological process, etc. associated PubMed ID Person: Jie Zheng a instance-level relation between an entity to a PubMed ID that denotes a publication associated with the entity. is about organism species relates an information artifact to the species of an organism that it is about. Person: Jie Zheng is denoted by is denoted by is an instance-level, relation obtaining between an entity to an information content entity that is created to specifically refer to something. It is the inverse relation of 'denotes'. Person: Jie Zheng is about genotype relates an information artifact to the genotype of an organism that it is about. Person: Jie Zheng is about cell type relates an information artifact to the type of a cell that it is about. Person: Jie Zheng is about cell line relates an information artifact to the cell line that it is about. Person: Jie Zheng is about tissue Person: Jie Zheng relates an information artifact to the tissue of an organism that it is about. has gene member relates the member (a data item) of a gene list (a data set) to the gene that it is about. is about strain relates an information artifact to the strain of an organism that it is about. is about phenotype relates an information artifact to the phenotype born by an organism that it is about. is about development stage relates an information artifact to the development stage of an organism that it is about. is about treatment relates an information artifact to the treatment that it is about. For specimens in an assay, the treatment referred to was performed prior to specimen collection. has duration relates a process to a time-measurement-datum that represents the duration of the process has concentration relates a molecular entity to its concentration measurement datum that is the measurement of its concentration quality binding context relates a gene that has protein binding to the biological context in which that binding was identified. differential expression context relates a gene that is differentially expressed to the biological context in which that differential expression was identified. context of differential expression relates a biological context (e.g., cell, developmental stage, etc.) to a gene whose protein binding was identified in this specific context. context of binding relates the context to a gene that protein binding is identified in this specific context has NCBI gene id differential expression pattern mouse strain Mus musculus that is bred to have some uniform behavioral, morphological, physiological, or genetic characteristics with similarly bred organism. Person: Jie Zheng rat strain Person: Jie Zheng Rattus norvegicus that is bred to have some uniform behavioral, morphological, physiological, or genetic characteristics with similarly bred organism. insulin expressing cell a cell that expresses a gene for insulin. sequence feature quantification UPenn Group Person: Chris Stoeckert, Jie Zheng, Elisabetta Manduchi A data transformation that attaches quantified values to specific sequence features, e.g. transcript, exon, etc. high throughput sequence alignment EFO_0004917 high throughput sequence alignment protocol a data transformation that aligns sequencing reads to reference sequence. Person: Chris Stoeckert, Jie Zheng Tg(Ins1-EGFP/GH1)14Hara mouse strain A mouse strain containing a transgene in which EGFP (transgene) is driven by the Ins1 promoter (NCBI Gene ID: 16333). Hemizygous mice show expression of EGFP in beta lineage cells of the pancreas. MIP-GFP mouse strain Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler RES188 mouse strain STOCK Tg(Ins1-EGFP/GH1)14Hara/Mmmh mouse strain Tg(Ins1-EGFP/GH1)14Hara Vanderbilt University Medical Center C57BL/6 mouse strain A mouse strain that is a laboratory mouse generated from long inbreeding with nearly identical genotype. C57BL/6 mouse has a dark brown, nearly black coat, and an easily irritable temperament. It has a good reproductive performance and is the most widely used "genetic background" for genetically modified mice for use as models of human disease. black 6 C57 http://en.wikipedia.org/wiki/C57BL/6 Person:Jie Zheng C57 black 6 functional beta cell like cell A cell that has all features and genetic signatures as functional beta cell. It may not be part of pancreas. Sox17{(GFPCre)Mgn} mouse strain STOCK Sox17tm2(EGFP/cre)Mgn/Mmnc mouse strain RES1681 mouse strain Sox17tm1.3(Cre.GFP)Mgn mouse strain A mouse strain in which part or all of the targeted gene, SRY-box containing gene 17 (NCBI Gene ID: 20671) is replaced to create Sox17{GFPCre} using the Loxed Cassette Acceptor allele Sox17{tm1(LCA)}). Using an RMCE strategy, we inserted a GFP-Cre (Green fluorescent protein-Cre-recombinase) fusion protein into a Sox17 LCA allele thereby replacing Sox17 coding sequences. The Sox17{GFPCre} mice may be used to track Sox17-expressing cells and their early progeny, or to conditionally inactivate genes in Sox17-expressing cells. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Sox17{(GFPCre)Mgn} Vanderbilt University Medical Center Pdx1{(CFP)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{tm1.2(CFP)} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). This mouse contains a cyan fluorescent protein (CFP, Cerulean), tagged with 3 copies of an SV40 NLS knocked into the endogenous Pdx1 gene. This mouse strain enables pre-pancreatic endoderm to be easily identified in the developing mouse embryo and the isolation of Pdx1(+) cells by FACS. RES1301 mouse strain B6.129S6-Pdx1tm2Mgn/Mmnc mouse strain Pdx1CFP mouse strain Pdx1{(CFP)Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{tm1Mgn} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create ptf1a{tm1.1(YFP)} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). <p>Ptf1a{YFP} mice may be used to visualize yellow fluorescent protein (YFP) expression from the ptf1a allele. Ptf1a is expressedin pancreatic buds beginning at E9.5 andplays a vital role in the growth and lineage specification of pancreatic multipotent progenitor cells (MPCs). Ptf1a is also expressed in the neuronal precursors of the cerebellum, spinal cord, and retina where it also performs fate determining roles.</p> Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm1Mgn} STOCK Ptf1atm1.1Mgn/Mmmh mouse strain ptf1aYFP mouse strain RES236 mouse strain Vanderbilt University Medical Center B6.129S-Neurog3{tm1(EGFP)Khk}/Mmcd mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Gene Replacement) to targeted mutation 1, Klaus H Kaestner (MGI ID: Neurog3{tm1(EGFP)Khk}). A targeted mutation at the Neurogenin 3 locus where EGFP replaces entire coding region of Neurogenin3. The basic phenotype is EGFP expression in developing endocrine cells of pancreas and in glandular regions of the stomach. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler ngn3-EGFP mouse strain RES176 mouse strain B6.129S-Neurog3tm1(EGFP)Khk/Mmcd mouse strain B6.129S-Neurog3{tm1(EGFP)Khk}/Mmcd Vanderbilt University Medical Center Insm1tm2Mgn mouse strain RES661 mouse strain http://www.betacell.org/resource/view/resource_id/661/ Person: Jie Zheng, Chris Stoeckert http://www.betacell.org/resource/view/resource_id/661/ provide the definition of 'Insm1tm1.1Mgn mouse strain' which might be the early version of 'Insm1tm2Mgn mouse strain'. However, we are not clear the difference between two strains. We borrowed the definition of 'Insm1tm1.1Mgn mouse strain'. In the webpage, it has strain name 'Mouse Strain RES661' and common name 'lnsm1GFP.Cre mouse strain'. We added 'lnsm1GFP.Cre mouse strain' as alternative term which is true to 'Insm1tm2Mgn mouse strain' too . A mouse strain contains a GFP-Cre fusion protein which replaces the Insm1 coding sequence. These mice express green fluorescent protein (GFP) under control of the Insm1 gene locus. Insm1 is expressed in pancreatic primordium starting at E9.5. Insm1 is also expressed in neural precursor cells and tumors of may be used for lineage tracing of Insm1-positive cells in both wild-type and Insm1-null mice. Insm1GFP.Cre mouse strain obsolete_STOCK Tg(Tnpo1-EGFP)6729Hara/Mmmh mouse strain true http://purl.obolibrary.org/obo/BCGO_0000012 http://www.betacell.org/resource/view/resource_id/188/ A mouse strain which is hemizygous and shows expression of EGFP in beta lineage cells of the pancreas. RES188 mouse strain Person: Jie Zheng MIP-GFP mouse strain 129X1/SvJ mouse strain A mouse strain that is a laboratory mouse generated from inbreeding. It has white-bellied, pink-eyed, light chinchilla (off-white) or albino or white-bellied, pink-eyed, light chinchilla (light tan). It has a good reproductive performance and is widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. Person:Jie Zheng http://www.informatics.jax.org/mgihome/nomen/strain_129.shtml http://jaxmice.jax.org/strain/000691.html 129X1 129/SvJ C3H mouse strain A mouse strain that Inbred from F130 to F170 depending on substrain original generated from a cross of a Bagg albino female and a DBA male by Strong in 1920. It has Agouti coat color. Most substrains have a good reproductive performance. It is widely used in a wide variety of research areas including cancer, immunology and inflammation, sensorineural, and cardiovascular biology. Person:Jie Zheng http://www.informatics.jax.org/external/festing/mouse/docs/C3H.shtml http://www.criver.com/EN-US/PRODSERV/BYTYPE/RESMODOVER/RESMOD/Pages/C3HMouse.aspx black swiss mouse strain Person: Jie Zheng Sox17-expressing definitive endodermal cell Person: Chris Stoeckert, Jie Zheng Chris Stoeckert Sox17 expressing cell from the embryo body proper an endodermal cell that is part of the definitive endoderm and expresses a gene for SOX-17 Sox17-expressing Epcam+ pancreatic epithelial cell an epithelial cell that is part of the ventral pancreatic bud, expresses a gene for SOX-17 and has Epcam in the plasma membrane. Person: Chris Stoeckert, Jie Zheng Sox17 expressing cell in ventral pancreatic bud Chris Stoeckert Pdx1-expressing foregut endodermal cell Pdx1-expressing endoderm cell an endodermal cell that is part of the foregut endoderm and expresses the gene for pancreas/duodenum homeobox protein 1 Person: Chris Stoeckert, Jie Zheng Chris Stoeckert Ptf1a-expressing multipotent pancreatic cell Chris Stoeckert Person: Chris Stoeckert, Jie Zheng a non-terminally differentiated cell that is part of the pancreatic bud, participates in pancreas development and expresses the gene for pancreas transcription factor 1 subunit alpha. Ptf1a-expressing cell Ptf1a-lacking pancreatic progenitor cell Ptf1a-null cell Chris Stoeckert a non-terminally differentiated cell that is part of the pancreatic bud but lacks pancreas transcription factor 1 subunit alpha. Person: Chris Stoeckert, Jie Zheng neurogenin-3-expressing endocrine progenitor cell Chris Stoeckert Ngn3-expressing cell a non-terminally differentiated cell that participates in endocrine pancreas developement and expresses the gene for neurogenin-3. Person: Chris Stoeckert, Jie Zheng neurogenin-3-lacking pancreatic progenitor cell Chris Stoeckert a non-terminally differentiated cell that is part of the pancreas, participates in pancreas development and lacks neurogenin-3. Ngn3-null cell Person: Chris Stoeckert, Jie Zheng Insm1-expressing endocrine progenitor cell Chris Stoeckert a non-terminally differentiated cell that participates in endocrine pancreas development and expresses a gene for insulinoma-associated protein 1 Person: Chris Stoeckert, Jie Zheng Insm1 expressing pancreatic endocrine cell Insm1-lacking endocrine progenitor cell Chris Stoeckert Person: Chris Stoeckert, Jie Zheng a non-terminally differentiated cell that is part of the endocrine pancreas and lacks insulinoma-associated protein 1 Non-functional Insm1 expressing pancreatic endocrine cell insulin-expressing immature beta cell Chris Stoeckert a type B pancreatic cell that is immature and expresses a gene for insulin Person: Chris Stoeckert, Jie Zheng insulin-expressing nascent beta cells insulin-expressing mature beta cell a type B pancreatic cell that is mature and expresses a gene for insulin Chris Stoeckert Person: Chris Stoeckert, Jie Zheng insulin-expressing mature beta cells Insm1-expressing Pdx1 high-positive pancreatic endocrine progenitor cell A Insm1-expressing endocrine progenitor cell that expresses the gene for pancreas/duodenum homeobox protein 1 at a high level. Person: Jie Zheng, Chris Stoeckert Insm1-expressing Pdx1 low-positive pancreatic endocrine progenitor cell Person: Jie Zheng, Chris Stoeckert A Insm1-expressing endocrine progenitor cell that expresses the gene for pancreas/duodenum homeobox protein 1 at a low level. Insm1tm2(GFP.Cre)Mgn/Pdx1tm2(CFP)Mgn mouse strain A mouse strain that is the result of mating between a Insm1GFPCre/+ mouse and a Pdx1CFP/+ mouse both on a CD-1 background Person: Chris Stoeckert, Anna Osipovich differential expression software GBCO A software implementing a higher level analysis protocol of type differential expresssion. B6.Cg-Tg(Ins1-EGFP)1Hara/J mouse strain A mouse strain that is genetically modified expressing Enhanced Green Fluorescent Protein (EGFP) under the control of the mouse insulin 1 promoter. Fluorescence is detected in tissues where insulin I is normally expressed; fluorescent protein expression in pancreatic beta-cells is evident from embryonic day (E)13.5 through adulthood. The fluorescence expression pattern is similar to the patterns seen in two other strains STOCK Tg(Ins1-Cerulean)24Hara/J and STOCK Tg(Ins1-DsRed*T4)32Hara/J. This mutant mouse strain exhits normal glucose tolerance and pancreatic insulin levels and may be useful in studies of diabetes and pancreatic beta islet cell biology. Person: Jie Zheng http://jaxmice.jax.org/strain/006864.html ArrayExpress ID A CRID symbol assigned by ArrayExpress to uniquely identify a study in ArrayExpress GEO ID A CRID symbol assigned by Gene Expression Omnibus (GEO) to uniquely identify a study in GEO. cellular marker expression Person: Chris Stoeckert, Jie Zheng Penn Group Beta Cell Biology Consoritum A gene expression that produces cellular surface proteins that can be used to identify and isolate specific cells. pancreatic material Beta Cell Biology Consoritum Person: Chris Stoeckert, Jie Zheng Penn Group A material entity that could be whole pancreas, part of pancreas (including a single cell type (e.g., beta cell), or precursor to pancreas. characterization of cell differentiation objective A cellular feature identification objective that aims to examine or characterize cell development or differentiation. Person: Chris Stoeckert, Jie Zheng Beta Cell Biology Consoritum Penn group characterization of differentiation of insulin-producing cells objective A characterization of cell differentiation objective that aims to examine the influence of specific protocols with regard to generating insulin-producing cells from putative progenitors or other mechanisms (e.g., transdifferentiation). Beta Cell Biology Consoritum Penn group Person: Chris Stoeckert, Jie Zheng characterization of islet/beta-cell stimulation/injury objective Penn group Beta Cell Biology Consoritum Person: Chris Stoeckert, Jie Zheng A characterization of cell stimulation or injury objective that aims to address the effects of compounds, hormones, or cell manipulations on the ability of islets and/or beta-cells to secrete insulin or respond to glucose levels. characterization of pancreas development and growth objective Penn group A biological feature identification objective that aims to examine changes in gene expression and/or epigenome during pancreas development or regeneration. Person: Chris Stoeckert, Jie Zheng Beta Cell Biology Consoritum characterization of cell stimulation or injury objective A cellular feature identification objective that aims to address the effects of compounds, hormones, or cell manipulations on the ability of tissues or specific cell types to perform their normal functions or respond to insult. Beta Cell Biology Consoritum Penn group Person: Chris Stoeckert, Jie Zheng targets and roles of transcriptional regulators objective A molecular feature identification objective that aims to identify genes and processes regulated by transcription factors, co-activators, and other types of transcriptional regulators. Person: Chris Stoeckert, Jie Zheng Penn group Beta Cell Biology Consoritum tissue expression survey objective Penn group Person: Chris Stoeckert, Jie Zheng A biological feature identification objective that aims to address the question of what genes are expressed in a given tissue or cell type or the expression of genes across multiple cells and tissues. Beta Cell Biology Consoritum epigenetic modification process Beta Cell Biology Consoritum A biological process of generating epigenetic modifications, such as DNA methylation and histone modifications. Penn Group Person: Chris Stoeckert, Jie Zheng islet expansion in vitro Penn Group Beta Cell Biology Consoritum A planned process that aims to expand islets in culture and redifferentiate them to insulin producing cells. Person: Chris Stoeckert, Jie Zheng islets in diabetes Person: Chris Stoeckert, Jie Zheng islet of Langerhans that is part of a person who has diabetes. Will add following axiom after import 'has disease' from CLO 'is part of' some ('Homo sapiens' and 'has disease' some 'diabetes mellitus') Penn Group Beta Cell Biology Consoritum long non-coding RNA http://en.wikipedia.org/wiki/Long_non-coding_RNA Beta Cell Biology Consoritum Person: Chris Stoeckert, Jie Zheng lncRNA A ncRNA which is transcribed, non-protein coding, and longer than 200 nucleotides. stress and apoptosis in islets or beta cells Beta Cell Biology Consoritum Penn Group Person: Chris Stoeckert, Jie Zheng transcription factor binding site identification by SACO assay Person: Chris Stoeckert, Jie Zheng Beta Cell Biology Consoritum An assay that uses chromatin immunoprecipitation combined with serial analysis of chromatin occupancy (SACO) to identify binding sites for transcription factors. TF Binding SACO Penn group transcriptional profiling by MARIS assay MARIS Person: Chris Stoeckert, Jie Zheng An assay that aims to measure abundance of transcripts using high-quality RNA isolated from cells following intracellular antibody staining and fluorescence-activated cell sorting (FACS). Beta Cell Biology Consoritum http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0089459 age datum A data item of age. It could be measurement datum like 10 weeks, 2 years, or categorical datum like young, adult. concentration datum A data item of how much of a given substance there is mixed with another substance.Annotations. It could be measurement datum like 10 mM, or categorical datum like low, high concentration. Black and tan, brachyury mouse strain Person: Jie Zheng, Chris Stoeckert BTBR PMID: 25034792, Nephron Exp Nephrol. 2014;126(4):191-5. doi: 10.1159/000363300. "Recent advances in animal models of diabetic nephropathy." A mouse strain that is deficient for the leptin receptor (ob/ob) which is naturally insulin resistant and develops many of the pathological features of human Diabetic nephropathy. large pancreatic ductal cell http://www.sciencedirect.com/science/article/pii/S1357272504002717 large duct cell Person: Chris Stoeckert A pancreatic ductal cell of the large pancreatic duct. small pancreatic ductal cell' http://www.sciencedirect.com/science/article/pii/S1357272504002717 Person: Chris Stoeckert small duct cell A pancreatic ductal cell of a small interlobular pancreatic duct. Tet-On/Ngn3 embryonic stem cell ES (Tet-On/Ngn3) Person: Jie Zheng, Chris Stoeckert Penn Group An embryonic stem cell line that contains a ‘tet-on’ operon system and expresses Ngn3 in the presence of Tetracycline. GKP2 cell An immortal animal cell line cell which was derived from an insulinoma and expresses insulin and pdx1. GKP2 Person: Jie Zheng, Chris Stoeckert BCBC (http://www.betacell.org/resource/view/resource_id/1038) GKP4 cell An immortal animal cell line cell which was derived from a periductal tumor that did not express insulin or pdx1 but expressed genes of the endocrine lineage, such as Isl1 and Nkx2.2. GKP4 BCBC (http://www.betacell.org/resource/view/resource_id/1038) Person: Jie Zheng, Chris Stoeckert Sox17 expressiong H9 cell A H9 cell that expresses Sox17. BCBC (http://www.betacell.org/resource/view/resource_id/4216) Person: Jie Zheng, Chris Stoeckert INS-1 (832/1 and 832/2) PMID:17185391 Person: Jie Zheng, Chris Stoeckert An INS-1 cell which is poorly responsive to glucose. It is a mixture of INS-1 (832/1) and INS-1 (832/2). INS-1 (832/1) cell Person: Jie Zheng, Chris Stoeckert PMID:17185391 An INS-1 cell which is poorly responsive to glucose. INS-1 (832/13 and 833/15) Person: Jie Zheng, Chris Stoeckert An INS-1 cell which is highly responsive to glucose. It is a mixture of INS-1 (832/13) and INS-1 (832/15). PMID:17185391 INS-1 (832/13) cell Person: Jie Zheng, Chris Stoeckert An INS-1 cell which is highly responsive to glucose. PMID:17185391 INS-1 (832/2) cell PMID:17185391 An INS-1 cellwhich is poorly responsive to glucose. Person: Jie Zheng, Chris Stoeckert INS-1 (833/117) cell An INS-1 cell which is cytokine resistant. Person: Jie Zheng, Chris Stoeckert BCBC INS-1 (833/15) cell An INS-1 cell which is cytokine resistant and highly glucose-responsive. BCBC Person: Jie Zheng, Chris Stoeckert INS-1 (834/40) cell An INS-1 cell which is cytokine sensitive. BCBC Person: Jie Zheng, Chris Stoeckert INS-1E An INS-1 cell which has high insulin secretion in responses to glucose. PMID: 10037448 Person: Jie Zheng, Chris Stoeckert mPAC L20 cell An immortal animal cell line cell which is a pancreatic duct cell and contains an Ngn3-expressing adenovirus. Person: Jie Zheng, Chris Stoeckert mPAC L20 PMID: 15340143 PANC-1 cell Person: Jie Zheng, Chris Stoeckert PANC-1 An immortal animal cell line cell which was established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. The cell possesses the type B phenotype for G6PD. http://www.sigmaaldrich.com/catalog/product/sigma/87092802 R7T1 cell Person: Jie Zheng, Chris Stoeckert PMID: 19164755 R7T1 An immortal animal cell line cell that is a rat beta-cell line. B6.CBA-{Tg(Gcg-cre)1Herr}/Mmnc mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Gcg promoter (NCBI Gene ID: 14526). <p>A transgenic alteration consisting of a SacI fragment containing the glucagon promoter, inserted at SacI of pBS-beta globin-Cre, resulting in Cre expression in pancreatic lineage cells.</p> B6.CBA-{Tg(Gcg-cre)1Herr}/Mmnc Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center B6.CBA-{Tg(Ipf1-EGFP)1Herr}/Mmnc mouse strain A mouse strain containing a transgene in which EGFP (transgene) is driven by the Ipf1 promoter (NCBI Gene ID: 18609). This strain allows Expression of EGFP in pancreatic precursor populations. B6.CBA-{Tg(Ipf1-EGFP)1Herr}/Mmnc Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center B6;D2-Tg(Ins-cre)23Herr mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Ins1 promoter (NCBI Gene ID: 24505). This strain relies on the recombination of floxed DNA in insulin-producing beta cells. B6;D2-Tg(Ins-cre)23Herr Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center B6;D2-Tg(NEUROG3-cre)1Herr mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). <p>All islet endocrine cells inherit a recombination event, if a segment of DNA is loxP-flanked. Transgene encoding Cre recombinase under the control of a human Neurogenin3 promoter.</p> B6;D2-Tg(NEUROG3-cre)1Herr Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center BX129-Neurod1{tmAK71(lacZ)Jle/Mmcd} mouse strain A mouse strain containing a transgene in which LacZ-Neo (transgene) is driven by the Neurod1 promoter (NCBI Gene ID: 18012). Homozygous phenotype: Postnatal lethal due to neonatal diabetes Pancreas--5% insulin production, no islet formation Gut--no CCK or secretin production Inner ear defects resulting in deafness--no cochlear ganglia and poor vestibular ganglia formation Cerebellar defect resulting in ataxia-- >90% depletion of cerebellar granule cells Hippocampal defects--no dentate gyrus. BX129-Neurod1{tmAK71(lacZ)Jle/Mmcd} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center STOCK Ipf1{tm1Cvw} mouse strain A mouse strain in which the targeted gene, insulin promoter factor 1, homeodomain transcription factor (NCBI Gene ID: 18609), has been modified (allele type: Global Null) to targeted mutation 1, Christopher V E Wright (MGI ID: Ipf1{tm1Cvw}). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Ipf1{tm1Cvw} Vanderbilt University Medical Center STOCK Ipf1{tm2Cvw}/Mmnc mouse strain A mouse strain in which the targeted gene, insulin promoter factor 1, homeodomain transcription factor (NCBI Gene ID: 18609), has been modified (allele type: Gene Replacement) to insulin promoter factor 1, homeodomain transcription factor / targeted mutation 2, Christopher V E Wright (MGI ID: Ipf1{tm2Cvw}). The homozygous phenotype of these animals show growth retardation and dehydration with lack of gastric emptying; ie. a-pancreatic. Heterozygous phenotype includes expression of beta-galactosidase in developing stomach, bile duct and cystic ducts and early pancreatic buds. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Ipf1{tm2Cvw}/Mmnc Vanderbilt University Medical Center STOCK Ptf1a{tm1(cre)Cvw} mouse strain A mouse strain in which the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213), has been modified (allele type: Gene Replacement) to targeted mutation 1.1, Christopher VE Wright (MGI ID: Ptf1a{tm1.1(cre)Cvw}). <p>Homozygous phenotype is altered pancreatic development with loss of exocrine acini.</p> Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Ptf1a{tm1(cre)Cvw} Vanderbilt University Medical Center STOCK Tg(Foxa3-cre)1Khk mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Foxa3 promoter (NCBI Gene ID: 15377). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Tg(Foxa3-cre)1Khk Vanderbilt University Medical Center STOCK Tg(Ipf1-cre/Esr1)1Dam/Mmcd mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Ipf1 promoter (NCBI Gene ID: 18609). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Tg(Ipf1-cre/Esr1)1Dam/Mmcd Vanderbilt University Medical Center STOCK-Tg(Ipf1-cre)89.1Dam/Mmcd mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Ipf1 promoter (NCBI Gene ID: 18609). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK-Tg(Ipf1-cre)89.1Dam/Mmcd Vanderbilt University Medical Center STOCK Tg(Neurog3-cre)1Dam/Mmcd mouse strain A mouse strain containing a transgene in which Cre (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Tg(Neurog3-cre)1Dam/Mmcd Vanderbilt University Medical Center Gck{tm3Mgn} mouse strain A mouse strain in which the targeted gene, Glucokinase (NCBI Gene ID: 103988), has been modified (allele type: Global Mutation) to targeted mutation 3 (MGI ID: Gck{tm3Mgn}). This line of mice provides a model for Persistant Hyperinsulinemic Hypoglycemia of Infancy, or PHHI-GK, a rare genetic disease of humans. A gk{A456V} mutation, originally identified in a human pedigree with PHHI-GK, was introduced into these mice by gene knock-in. These mice may be useful for studies of sustained hypoglycemia. The mutation has been bred into a C57BL/6J strain thereby facilitating direct comparisons to both wild type C57BL/6J animals and to animals with a gk{K414E} mutation. Gck{tm3Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gck{tm2Mgn} mouse strain A mouse strain in which the targeted gene, Glucokinase (NCBI Gene ID: 103988), has been modified (allele type: Global Mutation) to targeted mutation 2 (MGI ID: Gck{tm2Mgn}). This line of mice contains a gk{K414E} point mutation that was introduced by gene knock-in. This point mutation wasidentified in aMODY-GK pedigree. These mice will be useful for studies of sustained hyperglycemia since they contain only a single mutation and are congenic with the C57BL/6J strain. Gck{tm2Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gck{tm1.1Mgn} mouse strain A mouse strain in which the targeted gene, Glucokinase (NCBI Gene ID: 103988), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: Gck{tm1.1Mgn}). Gk{lox} mice may be used to generate cell specific knock-outs of glucokinase, depending which cre-expressing transgenic mouse is used. In humans, glucokinase gene mutations cause maturity onset diabetes of the young (MODY-GK), a disease that is characterized by early onset and persistent hyperglycemia. Thus, these mice are useful in determining how diminished experssion of glucokinase in specific cells causes hyperglycemia. Gck{tm1.1Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center pck{tm1.1Mgn} mouse strain A mouse strain in which the targeted gene, phosphoenolpyruvate carboxykinase 1 (NCBI Gene ID: 18534), has been modified (allele type: Conditional Null) to targeted mutation 1.1, Mark A Magnuson (MGI ID: Pck1{tm1.1Mgn} ). These mice may be used to study tissue-specific functions of phosphoenolpyruvate carboxykinase (Pck1). This enzyme is essential for gluconeogenesis and also is important for regulating anapleurosis/catapleurosis of TCA cycle intermediates. By generating mice that are homozygous for the pck{lox} allele and that contain a tissue-specific Cre transgene, Pck1 can be deleted from various sites. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center pck{tm1.1Mgn} Rik{tm1.3Mgn} mouse strain A mouse strain in which the targeted gene, Rictor (NCBI Gene ID: 78757), has been modified (allele type: Global Null) to targeted mutation 1.3 (MGI ID: 4921505C17Rik{tm2Mgn}). Rictor is an essential part of mTOR complex 2 (mTORC2). mTORC2 phosphorylates Ser473 of Akt/PKB. Eliminating this gene disrupts the function of mTORC2, thereby preventing growth factor mediated activation of Akt/PKB. Thus, these mice have utility for studying both the sites of expression and function of rictor. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rik{tm1.3Mgn} Vanderbilt University Medical Center Rik{tm1.1Mgn} mouse strain A mouse strain in which the targeted gene, Rictor (NCBI Gene ID: 78757), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: 4921505C17Rik{tm1.1Mgn}). These mice carrya conditional allele for Rictor, a component mTOR complex 2 (mTORC2). Disruption of the Rictor gene attenuates phosphorylation ofSer473 of Akt/PKB, which is important for a normal response to growth factor stimulation acting through the PI3-kinase signaling pathway. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rik{tm1.1Mgn} Vanderbilt University Medical Center Pparg{tm1.1Mgn} mouse strain A mouse strain in which the targeted gene, Peroxisome proliferator-activated receptor gamma (NCBI Gene ID: 19016), has been modified (allele type: Conditional Null) to targeted mutation 1.1Mgn (MGI ID: Pparg{tm1.1Mgn}). These mice carry a conditional allele for PPARgamma that can be used in combination with various cre-expressing transgenes. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Pparg{tm1.1Mgn} Vanderbilt University Medical Center B6.129X1-Abcc8{tm1.1Mgn} mouse strain A mouse strain in which the targeted gene, ATP-binding cassette, sub-family C (CFTR/MRP), member 8 (NCBI Gene ID: 20927), has been modified (allele type: Global Null) to targeted mutation 1.1Mgn (MGI ID: Sur1{neotm1.1Mgn}). These mice contain a global knock-out of the sulfonylurea receptor (Sur1), which is part of the ATP-dependent potassium channel. In humans, mutations in Sur1 cause persistent hyperinsulinemic hypoglycemia of infancy (PHHI). B6.129X1-Abcc8{tm1.1Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Tg(Pdx1-Neurog3-ER)1Agb mouse strain A mouse strain containing a transgene in which Neurog3-ER (transgene) is driven by the Pdx1 promoter (NCBI Gene ID: 18609). The transgene is driven by the Pdx1 promoter whcih targets it in pancreas progenitor during development. These mice express a fusion protein between the pro-endocrine transcription factor Ngn3 and the tamoxifen responsive estrogen receptor. An IRES site allows GFP to be co-expressed with Ngn3-ER. The protein is active only upon tamoxifen injection. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Tg(Pdx1-Neurog3-ER)1Agb Vanderbilt University Medical Center Glucagon:rtta mouse strain A mouse strain containing a transgene in which rtTA (transgene) is driven by the Gcg promoter (NCBI Gene ID: 14562). These mice expresses the rtTA (tetracycline transactivator) in islet alpha cells upon treatment with doxicycline. Glucagon:rtta Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center C57/CBAJ-Hprt{tm3(Ins2-HBEGF)Ugfm} mouse strain A mouse strain in which the targeted gene, hypoxanthine guanine phosphoribosyl transferase 1 (NCBI Gene ID: 15452), has been modified (allele type: Gene Replacement) to (MGI ID: ). C57/CBAJ-Hprt{tm3(Ins2-HBEGF)Ugfm} carries a transgene encoding the human diphtheria toxin receptor under the control of rat insulin II promoter, was inserted by homologous recombination at the Hprt locus of the X chromosome. It provides a model for depletion of 50% of beta cells using Diphtheria toxin. C57/CBAJ-Hprt{tm3(Ins2-HBEGF)Ugfm} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center B6-Nkx6-1{tm1Msan} mouse strain A mouse strain in which the targeted gene, Nkx6.1 (NCBI Gene ID: 18096), has been modified (allele type: Global Null) to targeted mutation (MGI ID: Nkx6.1{tm1(DsRed2)}). An IRES-DsRed2 cassette was introduced into exon 1 of the Nkx6.1 genomic sequence. The insertion generates a null allele. DsRed2 expression is not detected in these mice. B6-Nkx6-1{tm1Msan} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Tg(RIP-Cre/ESR1){Ydor} mouse strain A mouse strain containing a transgene in which CreER (transgene) is driven by the Ins promoter (NCBI Gene ID: 16333). A transgenic mouse expression tamoxifen-inducible Cre recombinase under rat insulin promoter. Upon the injection of tamoxifen, loxP-flanked DNA sequences in beta cells will be deleted. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Tg(RIP-Cre/ESR1){Ydor} Vanderbilt University Medical Center Ptf1a{tm1(tTAoff)Macd} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{tTA} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). Mice heterozygous for the tTA replacement of the Ptf1a gene are viable, fertile, of normal size, and do not display any behavioral abnormalities. The replacement places the expression of tTAoff (Gossen &amp; Bujard, PNAS 89:5547, 1992) under the control of the regulatory sequences of the endogenous Ptf1a gene. This mouse was initially designed to be mated to a mouse (see BCBC #M461) bearing a bicistronic Ptf1a-lacZ transgene driven by the tetracycline-regulatory element (TRE: 7 copies of tetO with the CMV minimal promoter). For offspring homozygous for the tTA allele of Ptf1a and hemi- or homozygous for the transgene, the production of PTF1a is due solely to tTA-activation of the transgene, which is repressed by administration of tetracycline/doxycycline. This strategy allows embryonic developmental arrest at desired stages or cessation of gene function in adult mice for the pancreas (similar to that for Pdx1 described in Holland et al., PNAS 99:12236, 2002) and also for the cerebellum, retina, dorsal spinal cord and possibly hypothalamus. This transgenic mouse may be useful in studies of pancreatic endocrine/exocrine development and function, diabetes, and certain defects of the CNS. This tissue-specific expresser of tTA can also be bred with strains bearing other TRE-transgenes for organ-specific conditional expression analyses. Nearly all of the Ptf1a transcription unit has been replaced by a tTAoff coding sequence with accessory translational and RNA-processing signals, as follows, from 5' to 3': the Ptf1a gene transcriptional start and 49-bp of its 5'UTR; the Xenopus laevis beta-globin 5'UTR with Kozak initiator codon; the tTA coding sequence; and a 990-bp rabbit beta-globin gene fragment encoding the last 14-bp of its second exon, the 573-bp second intron, and the 364-bp last exon including a 98-bp 3'UTR with the polyA addition signal and addition site, and 39-bp of 3' beta-globin gene flanking sequence. The Ptf1a locus was modified by recombination-mediated cassette exchange using the cassette exchange allele in Ptf1a-LCA ES cells (Burlison et al., submitted). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm1(tTAoff)Macd} Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(Ins2-HBEGF){6832Ugfm}/Sz mouse strain A mouse strain containing a transgene in which DTR (transgene) is driven by the RIP promoter (NCBI Gene ID: ). NOD-Prkdc{scid} IL2rg{null}Tg(Ins2-HBEGF){6832Ugfm} mice are deficient in mature lymphocytes and NK cells, survive beyond 16 months of age, and even after sublethal irradiation resist lymphoma development. They can be induced to become hypoglycemic when Diphtheria toxin is used. 100% of beta cells will be depleted upon administration of diphtheria toxin. NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(Ins2-HBEGF){6832Ugfm}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl}Hprt{tm3(Ins2-HBEGF)Ugfm}/Sz mouse strain A mouse strain containing a transgene in which DTR (transgene) is driven by the RIP promoter (NCBI Gene ID: ). NOD-scid IL2rg{null}Hprt{tm3(Ins2-HBEGF)Ugfm} mice are deficient in mature lymphocytes and NK cells, survive beyond 16 months of age, and even after sublethal irradiation resist lymphoma development. They can be induced to become hypoglycemic when Diphtheria toxin is used. 50% of beta cells will be depleted after diphtheria toxin administration. NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl}Hprt{tm3(Ins2-HBEGF)Ugfm}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Rag1{tm1Mom} Ins2{Akita} Prf1{tm1Sdz}/Sz mouse strain A mouse strain in which the targeted gene, recombination activating gene 1 (NCBI Gene ID: 19373), has been modified (allele type: Global Null) to (MGI ID: ). The NOD-Rag1{null} Prf1{null} Ins2{Akita} mouse is the first immunodeficient, spontaneously hyperglycemic mouse strain described that is based on the Ins2{Akita} mutation. This strain is suitable as hosts for human islet and human beta stem and progenitor cell transplantation in the absence of the need for pharmacological induction of diabetes. This strain of mice also has low levels of innate immunity and can be engrafted with a human immune system for the study of human islet allograft rejection. NOD.Cg-Rag1{tm1Mom} Ins2{Akita} Prf1{tm1Sdz}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Rag1{tm1Mom} Il2rg{tm1Wjl}/Sz mouse strain A mouse strain in which the targeted gene, interleukin 2 receptor, gamma chain (NCBI Gene ID: 16186), has been modified (allele type: Global Null) to (MGI ID: Il2rg{tm1Wjl}). Backcrossing of the Rag1 null allele onto the NOD/Lt strain background (NOD-Rag1{null} mice) provided a radio-resistant and longer-lived model for human-cell engraftment. Mutations in X-chromosome-linked Il2rg gene cause X-linked severe combined immunodeficiency (XSCID). Immunodeficient NOD-Rag1{null} IL2rg {null}mice tolerated much higher levels of irradiation conditioning than did NOD-Prkdc{scid} IL2rg {null} mice. NOD.Cg-Rag1{tm1Mom} Il2rg{tm1Wjl}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(Ins1-EGFP/GH1){14Hara}/Sz mouse strain A mouse strain in which the targeted gene, interleukin 2 receptor, gamma chain (NCBI Gene ID: 16186), has been modified (allele type: Global Null) to targeted mutation 1 (MGI ID: Il2rg{tm1Wjl}). NOD.Cg-Prkdc{scid}Il2rg{tm1Wjl}Tg(Ins1-EGFP/GH1){14Hara}/Sz is a immunodeficient mouse carries a GFP transgene under the control of mouse insulin promoter 1, which leads to expression of eGFP in the beta cells. NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(Ins1-EGFP/GH1){14Hara}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Neurog3{tm1Ggr} mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Other) to targeted mutation 1 (MGI ID: Ngn3{EYFP/+}). A targeting construct was designed to insert an IRES-EYFP and a floxed puro downstream of the coding sequence. Crossing with mice expressing Cre in the germ line excised the puromycin resistance gene. Coding region for Ngn3 is kept intact consequentely homozygous mice express Ngn3 protein , do not develop diabetes and behave like wild-types. in these mice, Ngn3-positive progenitors express EYFP and can be purified by FACS. Neurog3{tm1Ggr} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Tg(tetO-Ptf1a,lacZ)Macd mouse strain A mouse strain containing a transgene in which bicistronic Ptf1a-lacZ (transgene) is driven by the TRE promoter (NCBI Gene ID: ). Mice hemi- or homozygous for the transgene are viable, fertile, normal size, and do not display any behavioral abnormalities. Expression of the bicistronic transgene is directed by a heptameric tetO repeat linked to the CMV minimal promoter (collectively the tetracycline-response element). The mice do not express lacZ until a tetracycline-gransactivator (tTA) protein is produced; thereafter Ptf1a and lacZ genes are highly expressed. This mouse was designed to be mated to an apancreatic targeted mutant with tTAoff in place of the Ptf1a coding sequence (see BCBC strain M321). The combined genetic alterations provide normal pancreatic development and function until doxycycline-administration render the mice conditionally null of Ptf1a. This approach allows embryonic developmental arrest at desired stages or cessation of gene function in adult mice for the pancreas, cerebellum, retina, dorsal spinal cord and possibly hypothalamus. This transgenic mouse may be useful in studies of pancreatic endocrine/exocrine development and function, diabetes, and and certain defects of the CNS. This transgenic can also be bred with other tTA strains for conditional mutation analysis. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Tg(tetO-Ptf1a,lacZ)Macd Vanderbilt University Medical Center Ptf1a{tm2(W298A)Macd} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{tm2(W298A)} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). This mouse strain has a 2-bp site-directed mutation in the endogenous Ptf1a locus that changes codon 298 from tryptophan to alanine. The mutation disrupts the ability of the PTF1a protein to bind RBPJ. Mice heterozygous for the mutation are viable, fertile, of normal size, and do not display any behavioral abnormalities. Mice homozygous for the mutation phenocopy the homozygous Ptf1a-null. Newborn homozygotes are apancreatic, devoid of the dI4 and dILA GABAergic dorsal interneurons, have an incomplete cerebellum, and die shortly after birth. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm2(W298A)Macd} Vanderbilt University Medical Center Rbpjl{tm1(lacZ)Macd} mouse strain A mouse strain in which the targeted gene, Recombination signal binding protein for immunoglobulin kappa J region-like (NCBI Gene ID: 19668), has been modified (allele type: Global Mutation) to Rbpjl{tm1Macd} (MGI ID: Rbpjl{tm1(lacZ)}). This mouse strain has the Rbpjl gene region spanning exons 7, 8, and 9 replaced with lacZ in-frame. A pgk-neo resistance cassette has been removed by Cre-loxP deletion from the founder. Mice heterozygous and homozygous for the lacZ replacement are viable, fertile, of normal size, and do not display any behavioral abnormalities. In mature mice, the pancreas is one-third smaller than normal, due to less acinar tissue. Expression of the lacZ, measured by bet-galactosidase histostaining is very low. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rbpjl{tm1(lacZ)Macd} Vanderbilt University Medical Center STOCK Pdx1{tm1Macd}/J mouse strain A mouse strain in which the targeted gene, Pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609), has been modified (allele type: Gene Replacement) to targeted mutation 1 (MGI ID: Pdx1{tmd1Macd}). Mice homozygous for the targeted mutation fail to develop a pancreas. Heterozygous mice have normal pancreatic development, but have partially impaired glucose tolerance in adulthood. The substitution of the targeted Ipf1/Pdx1 gene with tTAoff inactivates the endogenous allele and places tTAoff expression under the control of the endogenous transcriptional regulatory sequences of the Pdx1 locus. Identical to the endogenous allele, mutant locus expression is detectable in the pancreas and adjacent duodenum but not in other visceral organs or salivary glands. This mutant may be useful to direct tetracycline-regulated expression of responder transgenes in studies of pancreatic endocrine/exocrine development and function and diabetes. This mutant can also be bred with other tetO/TRE strains for pancreas-specific applications. This mutant was originally designed to be mated with mice engineered with a heptameric tetracycline operator (tetO)-controlled bicistronic transgene coding for a normal PDX1 protein and with a beta-galactosidase or EGFP reporter (see BCBC mouse M561). The combined modifications allow normal pancreatic development and function until doxycycline-administration renders the mouse conditionally null of the Pdx1 gene. This configuration for conditional expression of Pdx1is most effective when the transgene locus is homozygous. This allows embryonic developmental arrest at desired stages or cessation of function in adult mice by tetracycline administration. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Pdx1{tm1Macd}/J Vanderbilt University Medical Center STOCK Tg(tetO-Pdx1,lacZ)958.1Macd mouse strain A mouse strain containing a transgene in which bicistronic Pdx1-lacZ (transgene) is driven by the TRE promoter (NCBI Gene ID: ). Mice hemizygous and homozygous for the transgenic insert are viable, fertile, of normal size, and do not display any behavioral abnormalities. Expression of the bicistronic transgene is directed by a heptameric tetO repeat fused to a minimal promoter (collectively the tetracycline-response element, or TRE). Transgenic mice do not express lacZ until a tetracycline-transactivator (tTA) protein is introduced; thereafter Pdx1 and lacZ from the transgene are expressed. All cells expressing transgenic Pdx1 coexpress the reporter. Further, mRNA levels of the transgene and endogenous Pdx1 fluctuate in concert during development. This mouse was originally designed to be mated to an apancreatic targeted mutant with the coding sequence of the Pdx1 locus replaced with that for tTAoff (see BCBC #M541). The combined mutations allow normal pancreatic development and function until doxycycline treatments render the mouse conditionally null of the endogenous gene. For this configuration, rescue of pancreatic development is most effective when the transgene locus is homozygous. This allows embryonic developmental arrest at desired stages or cessation of function in adult mice by administration of tetracycline/doxycycline. This transgenic strain may be useful in studies of pancreatic development and endocrine/exocrine function and diabetes. This strain can also be bred with other tTA strains for regulating Pdx1 expression in other contexts. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler STOCK Tg(tetO-Pdx1,lacZ)958.1Macd Vanderbilt University Medical Center Neurog3{tm2(EGFP)Ggr} mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Other) to targeted mutation 2 (MGI ID: Neurog3{tm2(EGFP)Ggr). A targeting construct was designed to insert an IRES-EGFP and a floxed puro downstream of the coding sequence. Crossing with mice expressing Cre in the germ line excised the puromycin resistance gene. Homozygous mice express Ngn3 protein, do not develop diabetes and behave like wild-types. Ngn3-positive cells are labeled with EGFP and can be purified by FACS. Neurog3{tm2(EGFP)Ggr} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Tg(Neurog3-IRES-NLS-LacZ)1Ggr mouse strain A mouse strain containing a transgene in which LacZ (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). Transgenic mice where Ngn3-positive cell are labeled with beta-galactosidase. Due to the stability of beta-galactosidase protein, the early descendants of Ngn3-positve cells are also labeled. To generate the Ngn3 promoter::LacZ construct, a 6.86 kb XbaI-XhoI fragment (6696 bp of 5' genomic and untranslated region sequences and 176 bp of ngn3 coding region) of mouse ngn3 genomic DNA was cloned upstream of the IRES-NLS-LacZ-pA sequence in pBS-INL vector resulting in plasmid pngn3(6.8)-INL. The 10.6 kb Ngn3-IRES-NLS-LacZ insert was released by NotI digestion and microinjected into murine oocyte pronuclei, and transgenic line was generated and maintained by crossing into a CD1 outbred background. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Tg(Neurog3-IRES-NLS-LacZ)1Ggr Vanderbilt University Medical Center Tg(Ins1-mRFP)2Ggr mouse strain A mouse strain containing a transgene in which mRFP (transgene) is driven by the Ins1 promoter (NCBI Gene ID: 1633). These transgenic mice express the monomeric red fluorescent protein under the control of Ins1 gene (BAC) regulatory elements. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Tg(Ins1-mRFP)2Ggr Vanderbilt University Medical Center Arx{tm1Pgr} mouse strain A mouse strain in which the targeted gene, Arx (NCBI Gene ID: 11878), has been modified (allele type: Gene Replacement) to targeted mutation 1 (MGI ID: Arx{tm1Pgr}). These mice were created by insertion of the LacZ gene (with nuclear localization signal) into the Arx locus by homologous recombination in embryonic stem cells. The Arx gene is localized on the X chromosome. Knockout mice die after birth with due to hypoglycemia. Therefore male mice die at day 2 past birth. The colony has to be propagated with female mice. Arx-deficient mice do not produce alpha cells in the islets of Langerhans. Arx{tm1Pgr} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Mafa{tm1.1Rwst} mouse strain A mouse strain in which the targeted gene, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (NCBI Gene ID: 378435), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: Mafa{tm1.1(lox)}). Mafa is a basic leucine-zipper containing member of the large Maf transcription factor family.It is part of the RIPE3b1 activator complex and functions as a key activator of insulin and pdx-1 gene transcription. Mafa{lox} mice may be used to generate both global and cell-specific Mafa null mice, depending on which cre-expressing transgenic mouse is used. Removal of Mafa gene might have a profound effect on beta cell function; thus we can closely monitor the expression of islet hormones, transcription functions and the glucose sensing machinery immunohistochemically. The experiment will provide us with the information about the role of Mafa plays in vivo in islet beta cells and the developing pancreas, thus helping us to understand how transcription activator contribute to the pathogenesis and the treatment of diabetes. Mafa{tm1.1Rwst} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Myt1{tm1.1Ggu} mouse strain A mouse strain in which the targeted gene, Myt1 (myelin transcriptioin factor 1) (NCBI Gene ID: 1100535 ), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: Myt1{tm1.1Ggu}). Two loxP sites flank exon 6 of Myt1b. The allele behaves as wild type allele without Cre-mediated deletion. Cre-mediated deletion creates null Myt1 allele. The homozygous null is neonatal lethal. Myt1 null pancreas has abnormal islet cell differentiation, ie. single cells produce multiple hormones. Pancreatic-specific Myt1 deletion results in glucose intolerance in males. Myt1{tm1.1Ggu} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center OC-1{tm1.1Mga} mouse strain A mouse strain in which the targeted gene, Onecut1 (NCBI Gene ID: 15379), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: OC-1{tm1.1Mga}). This line of mice allows for conditional inactivation of the OC-1 (HNF6) gene using Cre recombinase. Thus, HNF6 function can be assessed in the different tissues in which it is expressed. OC-1{tm1.1Mga} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2-2{tm3Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2(TNmut) using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). These mice contain mutations in the central core of the Nkx2.2 TN domain (putative groucho-interaction domain). Nkx2-2{tm3Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2-2{tm2Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2:rtTA using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). The reverse tetracycline transactivator (rtTA) is knocked into the Nkx2.2 coding region, effectively knocking out one Nkx2.2 allele. Nkx2-2{tm2Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center C57/CBAJ-TgN(Glucagon- hHB EGF)Ugfm mouse strain A mouse strain containing a transgene in which Diphtheria Toxin Receptor (transgene) is driven by the Gcg promoter (NCBI Gene ID: 14526). Transgenic strain (generated by pronuclear microinjection) of a transgene containing the rat glucagon promoter (1.6kb fragment) upstream of a rabbit beta-globin intron and the coding sequence of the human diphtheria toxin receptor (heparin-binding epidermal growth factor, membrane form). Downstream, there is a rabbit beta-globin stop codon and the polyadenylation signal. C57/CBAJ-TgN(Glucagon- hHB EGF)Ugfm Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center C57/CBAJ-TgN(Glucagon- rtTA)Ugfm mouse strain A mouse strain containing a transgene in which rtTA (transgene) is driven by the Gcg promoter (NCBI Gene ID: 14526). Transgenic strain (generated by pronuclear microinjection) of a transgene containing the rat glucagon promoter (1.6kb fragment) upstream of the coding region of the reverse transactivator doxycycline-dependent. The rtTA is flanked by a rabbit beta-globin intron and downstream there is a stop and polyA signal from the same rabbit beta-globin gene. C57/CBAJ-TgN(Glucagon- rtTA)Ugfm Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center C57/CBAJ-Tg(Ins2- HBEGF){6832Ugfm} mouse strain A mouse strain containing a transgene in which DTR (transgene) is driven by the Ins promoter (NCBI Gene ID: 16333). Transgenic mice generated by pronuclear microinjection of a transgene encoding the human diphtheria toxin receptor (heparin binding epidermal growth factor, also termed DTR) under the control of the rat insulin II gene promoter (0.6kb-long fragment). The DTR coding sequence is flanked by the rabbit beta-globin intron and stop/polyA sequences. C57/CBAJ-Tg(Ins2- HBEGF){6832Ugfm} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center FVB/NJ-Tg(MIP-Luc-VU)3Pwrs/J mouse strain A mouse strain containing a transgene in which Luc (transgene) is driven by the MIP promoter (NCBI Gene ID: 16333). We generated a transgenic mouse expressing the luciferase optical reporter under control of the mouse insulin I promoter (MIP-Luc-VU) and characterized this model in mice with increased or decreased beta-cell mass and after islet transplantation. MIP-Luc-VU mice emitted strong and consistent bioluminescence emanating exclusively from beta-cells of the pancreatic islet. MIP-Luc-VU islets had normal islet architecture and secreted insulin normally in vivo and in vitro. By tracking changes in &beta; cell mass using bioluminescence imaging (BLI) and post-mortem metrics, streptozotocin-induced, diabetic MIP-Luc-VU mice had a progressive decline in bioluminescence that correlated with a decrease in pancreatic insulin content and beta-cell mass. MIP-Luc-VU animals fed a high fat diet displayed a progressive increase in bioluminescence that reflected an immunohistochemically verified increase in beta-cell mass. MIP-Luc-VU islets transplanted beneath the renal capsule or into the liver emitted bioluminescence proportional to the number of islets transplanted and graft insulin content and could be imaged for more than a year. Since bioluminescence in the MIP-Luc-VU mouse model is proportional to beta-cell mass in the setting of increased and decreased beta-cell mass and after transplantation, this approach should be useful for non-invasively assessing beta-cell mass in pre-clinical mouse models of glucose homeostasis, beta-cell growth and regeneration, and diabetes. FVB/NJ-Tg(MIP-Luc-VU)3Pwrs/J Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gt(ROSA)26Sor{tm1(Myt1*)Hri} mouse strain A mouse strain in which the targeted gene, ROSA26 (NCBI Gene ID: 14910), has been modified (allele type: Conditional Activating) to ROSA26-loxp-STOP-loxp-Myt1 (MGI ID: Not yet available). Targeted insertion of a LoxP-STOP-LoxP-Myt1 casette into the ROSA26 locus. The presence of Cre recombinase will lead to activation of the 7 zinc finger isoform of Myt1. Gt(ROSA)26Sor{tm1(Myt1*)Hri} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ngn3{tm1(tTA)Hri} mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Gene Replacement) to Ngn3 tTA knockin (MGI ID: Ngn3tTA). The mouse contains a Ngn3 knock-in allele. IRES-tTA-rabbit beta globin polyA signal replaced the entire Ngn3 cDNA coding sequence. This is therefore also a Ngn3 null allele. Ngn3 is a bHLH transcription factor that is required for the differentiation of endocrine islet and some neuronal cells. Ngn3{tm1(tTA)Hri} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2-2{tm1Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2:LacZ using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). The lacZ gene has been knocked in to the Nkx2.2 genomic locus using RMCE, effectively replacing the two coding exons. Nkx2-2{tm1Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2-2{tm4Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2-NK2 mutation using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). The strain carries a mutant Nkx2.2 allele in which conserved residues of the NK2 domain are mutated to alanines. Nkx2-2{tm4Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{tm6Cvw} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{AIId} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). This is a targeted deletion of Area II 5' cis-regulatory region of Pdx1. Pdx1{tm6Cvw} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{tm4Cvw} mouse strain A mouse strain in which the targeted gene, Pancreatic and Duodenal homeobox 1 (NCBI Gene ID: 18609), has been modified (allele type: Conditional Null) to targeted mutation 4 (MGI ID: Pdx1{tm4(E2)}). This is a conditional null allele of Pdx1. LoxP sites for Cre recombinase were inserted to flank exon 2 of the gene. Pdx1{tm4Cvw} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{tm2(cre/ESR1)Cvw} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{tm2(cre/ESR1)} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). CreER knock-in into Ptf1a locus replacing part of the exon1 at the translation initiator ATG position using RMCE method. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm2(cre/ESR1)Cvw} Vanderbilt University Medical Center Pdx1{tm5Cvw} mouse strain A mouse strain in which the targeted gene, Pancreatic and Duodenal homeobox 1 (NCBI Gene ID: 18609), has been modified (allele type: Other) to targeted mutation 5 (MGI ID: Pdx1{tm5(AI-II-III)}). LoxP sites for cre recombinase were inserted to flank Area I-II-III, a conserved 5' cis-regulatory region of Pdx1. This allele was shown to be a conditional hypomorph by Fujitani et al. (2006) (PMID 16418487). Pdx1{tm3Cvw} (Pdx1{deltaI-II-III}) is generated by Cre-mediated recombination in the germline of this allele. Pdx1{tm5Cvw} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Dll1{tm1Hri} mouse strain A mouse strain in which the targeted gene, Delta-like 1 (NCBI Gene ID: 13388), has been modified (allele type: Conditional Null) to targeted mutation 1 (MGI ID: Dll1{tm1Hri}). This is a targeted mutation of the Notch ligand Dll1, introducing two loxP sites flanking the exons encoding the DSL domain. Dll1{tm1Hri} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{tm3Cvw} mouse strain A mouse strain in which the targeted gene, Pancreatic and Duodenal homeobox 1 (NCBI Gene ID: 18609), has been modified (allele type: Global Mutation) to targeted mutation 3 (MGI ID: Pdx1{tm3(deltaI-II-III)). Area I-II-III, a conserved 5' cis-regulatory region of Pdx1, was removed by Cre mediated recombination in the germline of Pdx1{tm5Cvw} (Pdx1{Flox123}) by crossing to deleter Cre mice. This allele was shown to be a hypomorph. Pdx1{tm3Cvw} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{tm3Cvw} mouse strain A mouse strain in which the targeted gene, Pancreas Specific Transcription Factor, 1a (NCBI Gene ID: 19213), has been modified (allele type: Cassette Acceptor) to Targeted Mutation 3 (MGI ID: Ptf1a{tm3Cvw}). This mouse strain is a conditional null allele of Ptf1a. LoxP sites flanking exon 1 and 2 (entire coding region of Ptf1a). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm3Cvw} Vanderbilt University Medical Center Ptf1a{LCA} mouse ESC line A mouse ESC line in which the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Ptf1a{tm1(LCA)}). This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 4.1 kb region of this gene (including the proximal promoter and both exons 1 and 2) was replaced with a loxP site, an inverted loxP site, and both positive and negative selectable markers. This enables the use of Recombinase-Mediated Cassette Exchange (RMCE) to easily insert various reporter genes or to make other modifications of the Ptf1a gene locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{YFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{LCA} using the Loxed Cassette Acceptor allele Ptf1a{tm1.1(YFP)}). This ES cell line contains YFP inserted into a Ptf1a{LCA} allele by recombinase mediated cassette exchange. These cells may be useful for identifying pancreatic progenitor cells in cultures that have been stimulated to differentiate towards endodermal and pancreatic fates. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gck{LCA} mouse ESC line A mouse ESC line in which the targeted gene, glucokinase (NCBI Gene ID: 19213), has been modified (allele type: Cassette Acceptor) to targeted mutation 4 (MGI ID: gk{tm4(LCA)}). This ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the glucokinase locus by recombinase mediated cassette exchange (RMCE). This LCA uses a loxP/inverted loxP strategy for RMCE and allows sequences between - 6 kb and + 5 kb (relevative to the hepatic-specific promoter) to be manipulated. This enables a variety of different types of experiments to be performed using a range of cassette designs. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Insm1{LCA} mouse ESC line A mouse ESC line in which the targeted gene, insulinoma-associated 1 (NCBI Gene ID: 53626), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Insm1{tm1(LCA)}). This ES cell line contains a loxed cassette acceptor(LCA)allele that may be utilized for the exchange of DNAs of interest into the Insm1 gene locus by recombinase mediated cassette exchange (RMCE). This LCA uses a lox 66/71 and lox 2272 strategy for RMCE and allows a ~5.2 kb region of this single exon gene to be manipulated. This enables a variety of different types of experiments to be performed using a range of cassette designs. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{LCA} mouse ESC line A mouse ESC line in which the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Nkx2.2{tm1(LCA)}). This ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Nkx2.2 locus by recombinase mediated cassette exchange (RMCE). This LCA uses a Lox71/66 and Lox2272 strategy for RMCE and allows for manipulation of a 5.1 kb region of the gene (contains promoter, exon 1, and exon 2). This leads to a variety of experiments that can be performed using a wide range of cassette designs. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center MIP-GFP mouse ESC line A mouse ESC line containing a transgene in which Gfp (transgene) is driven by the Ins1 promoter (NCBI Gene ID: 16333). New ESC line derived from MIP-GFP mice (Hara et al. 2003,Am J Physiol Endocrinol Metab. 284: E177-83). Genetic background is C57Bl6/J. When injected into E3.5 mouse blastocysts this line contributes to chimeras and Insulin+/GFP+ double positive cells are found in E14.5 embryonic pancreas. No GFP+ cells are seen outside the pancreas. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ngn3-GFP mouse ESC line A mouse ESC line containing a transgene in which Gfp (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). Mouse ESC line derived from Ngn3-GFP transgenic mice (Gradwohl et al. unpublished). Genetic background is CD1. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{LCA} mouse ESC line A mouse ESC line in which the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Rosa26{tm1(LCA)}). This ES cell line contains a loxed cassette acceptor (LCA) allele in which a 5.17 kb region of the gene has been replaced by a Lox66 site, a puromycin-(delta)-thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter, a kanamycin resistance gene driven by the bacterial EM7 promoter, and a Lox2272 site. These features enable use of Recombinase-Mediated Cassette Exchange for the rapid insertion of various DNAs into the Rosa26 gene locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Sox17{LCA} mouse ESC line A mouse ESC line in which the targeted gene, SRY-box containing gene 17 (NCBI Gene ID: 20671), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Sox17{tm1(LCA)}). This mouse ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Sox17 gene locus by recombinase mediated cassette exchange (RMCE). A 3.79 kb region of the gene (exons 3-5) was replaced by tandemly oriented lox66 and lox2272 sites as well as both positive and negative selectable markers. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat IAPP raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "IAPP" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat C-peptide 2 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "C-peptide 2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat C-peptide 1 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "C-peptide 1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse C-peptide 1 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "C-peptide 1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Insulin raised in mouse A monoclonal antibody raised in mouse that targets the human "Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat PDX1 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat PDX1 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat Nkx6.1 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Nkx2.2 raised in goat A polyclonal antibody raised in goat that targets the human "Nkx2.2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human ghrelin raised in goat A polyclonal antibody raised in goat that targets the human "ghrelin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Pax6 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Pax6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human b-catenin raised in goat A polyclonal antibody raised in goat that targets the human "b-catenin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse smooth muscle actin raised in mouse A monoclonal antibody raised in mouse that targets the mouse "smooth muscle actin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human amylase raised in rabbit A polyclonal antibody raised in rabbit that targets the human "amylase" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human c-myc raised in rabbit A polyclonal antibody raised in rabbit that targets the human "c-myc" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat Glut-2 raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "Glut-2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal bovine Carboxypeptidase A raised in rabbit A polyclonal antibody raised in rabbit that targets the bovine "Carboxypeptidase A" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Chromogranin A raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Chromogranin A" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Chromogranin A raised in mouse A monoclonal antibody raised in mouse that targets the human "Chromogranin A" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Cytokeratin 7 raised in mouse A monoclonal antibody raised in mouse that targets the human "Cytokeratin 7" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human PECAM-1 raised in mouse A monoclonal antibody raised in mouse that targets the human "PECAM-1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal aequorea victoria Green Fluorescent Protein raised in rabbit A polyclonal antibody raised in rabbit that targets the aequorea victoria "Green Fluorescent Protein" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal chicken Nkx2.2 raised in mouse A monoclonal antibody raised in mouse that targets the chicken "Nkx2.2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat ghrelin raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "ghrelin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal chicken Islet-1 raised in mouse A monoclonal antibody raised in mouse that targets the chicken "Islet-1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human GATA-4 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "GATA-4" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Glucagon raised in mouse A monoclonal antibody raised in mouse that targets the human "Glucagon" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Somatostatin raised in mouse A monoclonal antibody raised in mouse that targets the human "Somatostatin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Pancreatic Ducts raised in mouse A monoclonal antibody raised in mouse that targets the rat "Pancreatic Ducts" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Pancreatic Ducts raised in mouse A monoclonal antibody raised in mouse that targets the rat "Pancreatic Ducts" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Islet Delta Cells raised in rat A monoclonal antibody raised in rat that targets the rat "Islet Delta Cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Pancreatic Ducts raised in rat A monoclonal antibody raised in rat that targets the rat "Pancreatic Ducts" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pro-Insulin raised in mouse A monoclonal antibody raised in mouse that targets the human "Pro-Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ngn3 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ngn3 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Ngn3 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Ngn3 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Nkx6.1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Nkx6.1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Nkx6.1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Nkx6.1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human HNF6 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "HNF6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in goat A polyclonal antibody raised in goat that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in guinea pig A polyclonal antibody raised in guinea pig that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human ghrelin raised in rabbit A polyclonal antibody raised in rabbit that targets the human "ghrelin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human ghrelin raised in rabbit A polyclonal antibody raised in rabbit that targets the human "ghrelin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat PDX1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat PDX1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat PDX1 raised in mouse A monoclonal antibody raised in mouse that targets the rat "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Sox2 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Sox2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse FoxA2 raised in goat A polyclonal antibody raised in goat that targets the mouse "FoxA2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human E-cadherin raised in mouse A monoclonal antibody raised in mouse that targets the human "E-cadherin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Sox17 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Sox17" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Sox17 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Sox17" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Sox17 raised in goat A polyclonal antibody raised in goat that targets the human "Sox17" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Shh raised in goat A polyclonal antibody raised in goat that targets the mouse "Shh" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal e. coli beta Galactosidase raised in chicken A polyclonal antibody raised in chicken that targets the e. coli "beta Galactosidase" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal aequorea victoria Green Fluorescent Protein raised in mouse A monoclonal antibody raised in mouse that targets the aequorea victoria "Green Fluorescent Protein" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ptf1a raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Ptf1a" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse IA1 (Insm-1) raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "IA1 (Insm-1)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic duct cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic duct cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic duct cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic duct cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic exocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic exocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic exocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic exocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic endocrine cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic endocrine cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Alpha cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Alpha cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Alpha cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Alpha cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Definitive Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Definitive Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Definitive Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Definitive Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human T (Brachyury) raised in goat A polyclonal antibody raised in goat that targets the human "T (Brachyury)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Laminin raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Laminin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal chicken Pax6 raised in mouse A monoclonal antibody raised in mouse that targets the chicken "Pax6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Sox17{GFPCre} mouse ESC line A mouse ESC line in which part or all of the targeted gene, SRY-box containing gene 17 (NCBI Gene ID: 20671) is replaced to create Sox17{Cre-GFP} using the Loxed Cassette Acceptor allele Sox17{tm1(LCA)}). Using an RMCE strategy, we inserted a Cre-GFP (Green fluorescent protein) fusion protein into a Sox17[LCA] allele thereby replacing Sox17 coding sequences. The Sox17-CreGFP ESCs may be used to track Sox17-expressing cells and their progeny, or to conditionally inactivate genes in Sox17-expressing cells. Mark the expression of Sox17 with both Cre and GFP. Cre will enable lineage tracing using reporter alleles that are activated by cre recombination. GFP will enable direct visualization of Sox17 gene expression. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Insm1{tm1.1Mgn} mouse strain A mouse strain in which part or all of the targeted gene, insulinoma-associated 1 (NCBI Gene ID: 53626) is replaced to create Insm1{tm1.1(GFP-Cre)} using the Loxed Cassette Acceptor allele Insm1{tm1(LCA)}). <p>Insm1{GFPCre} mice contain a GFP-Cre fusion protein which replaces the Insm1 coding sequence. These mice express green fluorescent protein (GFP) under control of theInsm1gene locus. Insm1 is expressed in pancreatic primordium starting at E9.5. Insm1 is also expressed in neural precursor cells and tumors of may be used for lineage tracing of Insm1-positive cells in both wild-type and Insm1-null mice.</p> Insm1{tm1.1Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ghrl{LCA} mouse ESC line A mouse ESC line in which the targeted gene, Ghrelin (NCBI Gene ID: 58991), has been modified (allele type: Cassette Acceptor) to targeted mutation (MGI ID: Ghrl{LCA}). ES cells were modified by homologous recombination to replace a 6.912 kb region of the Ghrelin (GHRL) gene from 2.7 kb upstream of exon 1 to 0.5 kb downstream of exon 5 with tandemly oriented lox71 and lox2272 sites as well as both positive and negative selectable markers. The gene was replaced by tandemly oriented lox71 and lox2272 sitesas well as both positive and negative selectable markers. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ghrl{Cre-eGFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, Ghrelin (NCBI Gene ID: 58991) is replaced to create Ghrl{Cre-eGFP} using the Loxed Cassette Acceptor allele Ghrl{LCA}). Recombinase mediated cassette exchange (RMCE) was used to insert the Cre-eGFP fusion protein into the ghrelin locus using ES cells containing the Ghrl{LCA} allele. The Cre-eGFP protein uses the endogenous AUG start codon and polyadenylation sequence. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse BetaTC6 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "BetaTC6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gt(ROSA)26Sor{tm1(GCK*)Ydor} mouse strain A mouse strain containing a transgene in which GCK (transgene) is driven by the ROSA26 promoter (NCBI Gene ID: 14910). Glucokinase containing the Y214C activating mutation, causing severe persistent hyperinsulinemic hypoglycemia (Diabetes 53:2164-2168, 2004) was targeted to the ROSA26 locus, preceded by a lox-stop-lox sequence and followed by IRES-EGFP. Gt(ROSA)26Sor{tm1(GCK*)Ydor} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat CART raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "CART" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Mo ES-Derived Early Endoderm raised in rat A monoclonal antibody raised in rat that targets the mouse "Mo ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Hu ES-Derived Early Endoderm raised in mouse A monoclonal antibody raised in mouse that targets the human "Hu ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Hu ES-Derived Early Endoderm raised in mouse A monoclonal antibody raised in mouse that targets the human "Hu ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Hu ES-Derived Early Endoderm raised in mouse A monoclonal antibody raised in mouse that targets the human "Hu ES-Derived Early Endoderm" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Pancreatic duct cells raised in rat A monoclonal antibody raised in rat that targets the mouse "Pancreatic duct cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Pancreatic duct and acinar cells raised in rat A monoclonal antibody raised in rat that targets the mouse "Pancreatic duct and acinar cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Endothelial cells raised in rat A monoclonal antibody raised in rat that targets the mouse "Endothelial cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse BetaTC6 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "BetaTC6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse BetaTC6 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "BetaTC6" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Disp2 raised in mouse A monoclonal antibody raised in mouse that targets the human "Disp2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Disp2 raised in mouse A monoclonal antibody raised in mouse that targets the human "Disp2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Lrp11 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Lrp11" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Sez6l2 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Sez6l2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Sez6l2 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Sez6l2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human DDR1 raised in goat A polyclonal antibody raised in goat that targets the human "DDR1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human DDR1 raised in mouse A monoclonal antibody raised in mouse that targets the human "DDR1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Gpr39-1a raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Gpr39-1a" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat CART raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "CART" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat CART raised in mouse A monoclonal antibody raised in mouse that targets the rat "CART" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-60-DR5-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{R26-60-DR5-TA-Cerulean} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -60 to +81 were replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess retinoic acid responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-228-DR5-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Roas26{R26-228-DR5-TA-Cerulean} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -228 to +81 were replaced by a retinoic acid response element (DR5) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess retinoic acid responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{tm3Macd} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{tm3Macd} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). Modified allele of Ptf1a with a biotinylation Tag sequence attached to the amino terminus of Ptf1a. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm3Macd} Vanderbilt University Medical Center Sox17{tm1.2Mgn} mouse strain A mouse strain in which part or all of the targeted gene, SRY-box containing gene 17 (NCBI Gene ID: 20671) is replaced to create Sox17{CreERT2} using the Loxed Cassette Acceptor allele Sox17{tm1(LCA)}). Sox17{CreERT2} mice may be used either to track Sox17-expressing cells or their progeny or to conditionally inactivate genes in Sox17-expressing cells at specific time points by tamoxifen injection. This line is complementary to Sox17-CreGFP and may avoid possible interferences of expression in the extra-embryonic visceral endoderm. We plan to analyze the effects of a direct activation/deletion of the Wnt pathway in the endoderm by crossing the Sox17-CreERT2 with the gain- and loss-of-function of beta-catenin. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Sox17{tm1.2Mgn} Vanderbilt University Medical Center Polyclonal mouse Carboxypeptidase A1 (CPA1) raised in goat A polyclonal antibody raised in goat that targets the mouse "Carboxypeptidase A1 (CPA1)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Muc1 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Muc1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Cleaved Notch1 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Cleaved Notch1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Jagged1 raised in goat A polyclonal antibody raised in goat that targets the human "Jagged1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{CFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{CFP} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). This ES cell line contains cyan fluorescent protein (CFP, Cerulean variant) inserted into a Pdx1{LCA} allele by recombinase mediated cassette exchange. These cells may be useful for identifying pancreatic progenitor cells in cultures that have been stimulated to differentiate towards endodermal and pancreatic fates. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Gt(ROSA)26Sor{tm1(Ccnd1T286A)Stang} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{(Ccnd1T286A)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This strain expresses both mutant cyclin D1{T286A} and a cherry reporter under the regulation of a tetracycline-responsive bi-directional minimal CMV promoter (TRE-Tight; tetO). The cyclin D1-T286A mutant cannot be phosphorylated by GSK-3beta and is resistant to polyubiquitination. Therefore, cyclin D1-T286A remains nuclear throughout the cell cycle and has an extended half-life relative to wild-type cyclin D1. This strain represents an effective tool for generating inducible tissue-specific cyclin D1{T286A} mutants with a cherry reporter. Gt(ROSA)26Sor{tm1(Ccnd1T286A)Stang} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{tm1.1(R26-60-DR5-TA-Cerulean)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{tm1.1(R26-DR5-TA-Cerulean)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus. The Rosa26 promoter in this mouse was altered by replacing DNA sequences from -60 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. This mouse will facilitate studies of retinoic acid signaling in an intact animal. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm1.1(R26-60-DR5-TA-Cerulean)Mgn} Vanderbilt University Medical Center NOD.Cg-Rag1{tm1Mom}Ins2{Akita}Il2rg{tm1Wjl}/Sz mouse strain A mouse strain in which the targeted gene, recombination activating gene 1 (NCBI Gene ID: 19373), has been modified (allele type: Global Null) to (MGI ID: Rag1{tm1Mom}). Backcrossing of the Rag1 null allele onto the NOD/Lt strain background (NOD-Rag1{null} mice) provided a radio-resistant and longer-lived model for human-cell engraftment. Mutations in X-chromosome-linked Il2rg gene cause X-linked severe combined immunodeficiency (XSCID). Immunodeficient NOD-Rag1{null} IL2rg nullmice tolerated much higher levels of irradiation conditioning than did NOD-Prkdcscid IL2rg{null} mice. This immunodeficient mouse also develops spontaneous hyperglycemia based on the Ins2{Akita} mutation. NOD.Cg-Rag1{tm1Mom}Ins2{Akita}Il2rg{tm1Wjl}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid}Il2rg{tm1Wjl}/Sz mouse strain A mouse strain in which the targeted gene, interleukin 2 receptor, gamma chain (NCBI Gene ID: 16186), has been modified (allele type: Global Null) to targeted mutation 1 (MGI ID: Il2rg{tm1Wjl}). NOD-scid IL2rg{null} mice are deficient in mature lymphocytes and NK cells, survive beyond 16-month of age, and even after sublethal irradiation resist lymphoma development. Moreover, cytokine-mobilized human peripheral blood stem cells engraft at high levels in NOD-scid IL2rg{null} mice and develop into human CD3+CD4+ and CD3+CD8+ T cells, Ig+B cells, myeloid cells, NK cells and plasmacytoid dentritic cells. NOD.Cg-Prkdc{scid}Il2rg{tm1Wjl}/Sz Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{tm1.2(R26-228-DR5-TA-Cerulean)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{tm1.2((R26-228-DR5-Cerulean) using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). These mice were generated using RMCE to insert an exchange vector containing a modified Rosa26 promoter linked to a Cerulean fluorescent protein (CFP) reporter gene into mESCs containing a Loxed Cassette Acceptor (LCA) allele within the Rosa26 gene locus. The Rosa26 promoter in this mouse was altered by replacing DNA sequences from -228 to +81 with a multimerized retinoic acid response element (DR5) fused to a TATA box. This mouse will facilitate studies of retinoic acid signaling in an intact animal. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm1.2(R26-228-DR5-TA-Cerulean)Mgn} Vanderbilt University Medical Center Nkx2-2{tm5(null)Suss} mouse strain A mouse strain in which the targeted gene, Nkx2.2 (NCBI Gene ID: 18088), has been modified (allele type: Global Null) to Null (MGI ID: Nkx2.2{tm5(null)}). These mice contain a deletion of the Nkx2.2 coding region to create a null allele. Nkx2-2{tm5(null)Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx6-1{tm2(flox)Msan} mouse strain A mouse strain in which the targeted gene, Nk6 Homeobox 1 (NCBI Gene ID: 18096), has been modified (allele type: Conditional Null) to targeted mutation 1.1 (MGI ID: Nkx6.1{tm1.1Msan}). A conditional allele for nkx6.1 is necessary to study the function of Nkx6.1 in adult mice. A homozygous nkx6.1 null allele results in neonatal lethality due to a paralytic state, which is a direct result of Nkx6.1 function in the development of motor neurons. Conditional loss of Nkx6.1 is achieved through Cre mediated recombination of a floxed exon II of nkx6.1. Our use for a conditional nkx6.1 allele is to study its function in beta cells of the adult pancreas. Nkx6-1{tm2(flox)Msan} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Rag1{tm1Mom}Prf1{tm1Sdz}/SzJ mouse strain A mouse strain in which the targeted gene, Perforin 1 (Pore forming protein) (NCBI Gene ID: 18646), has been modified (allele type: Global Null) to (MGI ID: ). Mice that are homozygous for both targeted mutations are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities when housed under specific pathogen free conditions. These double homozygote mutant mice have no mature T or B lymphocytes, no detectable NK cell cytotoxic activity, and lack serum immunoglobulin. The number of nucleated spleen cells is significantly reduced in double mutant mice, when compared to the single homozygote, NOD.129S7(B6)-Rag1tm1Mom/J (Stock No. 003729). Although an increased number of DX5+CD122+ NK cells are found in the spleens of double mutants, these NK cells have impaired cytotoxic activity. The disruption of Prf1 ablates NK cell cytotoxic activity resulting in increased engraftment levels over that observed with Stock No. 003729. All mutant mice develop thymic lymphomas. This double mutant mouse strain may be useful in studies involving engraftment of human hematolymphoid cells. NOD.Cg-Rag1{tm1Mom}Prf1{tm1Sdz}/SzJ Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Foxo1{tm1(DBD)Dac} mouse strain A mouse strain in which part or all of the targeted gene, forkhead box O1 (NCBI Gene ID: 56458) is replaced to create Foxo1{DBD} using the Loxed Cassette Acceptor allele Foxo1{tm1(LCA)}). <p>This line contains a DNA binding-deficient mutant of Foxo1. The mutation may be useful for assessing the contribution of DNA co-activation in the function of Foxo1.</p> Foxo1{tm1(DBD)Dac} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{tm1(Ccnd1)} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{Ccnd1} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). ES cells express mutant cyclin D1-T286A and a cherry reporter under the regulation of a tetracycline-responsive bi-directional minimal CMV promoter (TRE-Tight; tetO) targeted to the ROSA26 locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-60-IBRE4-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{R26-60-IBRE4-TA-Cerulean} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -60 to +81 were replaced by a BMP response element (IBRE4) fused to a TATA-Cerulean (CFP) reporter. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-228-IBRE4-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{R26-228-IBRE4-TA-Cerulean} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -228 to +81 were replaced by a BMP response element (IBRE4) fused to a TATA-Cerulean (CFP) reporter. These ESCs may be useful for assess BMP responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Foxo1{tm1(GFP)Dac} mouse strain A mouse strain in which part or all of the targeted gene, forkhead box O1 (NCBI Gene ID: 56458) is replaced to create Foxo1{GFP} using the Loxed Cassette Acceptor allele Foxo1{tm1(LCA)}). A GFP cDNA has been knocked into the Foxo1 locus at the 3' end of the endogenous Foxo1 cDNA to generate a Foxo1-gfp fusion protein that can be used to study Foxo1 translocation in vivo. Foxo1{tm1(GFP)Dac} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse DNER raised in goat A polyclonal antibody raised in goat that targets the mouse "DNER" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Shh raised in mouse A monoclonal antibody raised in mouse that targets the rat "Shh" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal chicken Chick HNF3Beta raised in mouse A monoclonal antibody raised in mouse that targets the chicken "Chick HNF3Beta" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{LCA} mouse ESC line A mouse ESC line in which the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Pdx1{tm1(LCA)}). This mouse ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Pdx1 gene locus by recombinase mediated cassette exchange (RMCE). An 8.62 kb region of the gene was replaced by tandemly oriented Lox66 and Lox2272 sites flanking both positive (puromycin) and negative (HSV-TK)selectable markers. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Pax4 raised in guinea pig A polyclonal antibody raised in guinea pig that targets the mouse "Pax4" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN-Cherry-Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN-Cherry-Neo} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line expresses mCherry, a red fluorescent protein, under control of the endogenous Rosa26gene. A cassette containing mCherry and other sequences to assure efficient expression were inserted into the Rosa26[LCA] allele by recombinase mediated cassette exchange. These cells will be used to identify an optimal combination of regulatory elements for fluorescent protein expression from a single gene copy and as a reference cell line for fluorescent cell sorting. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Neurog3{tm1.1(nCre)Ggu} mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Gene Replacement) to Ngn3{nCre} (MGI ID: Neurog3{nCre}). A nCre-iRES (n-terminal half of Cre followed by internal ribosomal entry site) was inserted after Ngn3ATG. This allele is confirmed to express both nCre and Ngn3 (no visible phenotype in homozygous animals). Neurog3{tm1.1(nCre)Ggu} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{Non-EN-CFP-Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{LCA} using the Loxed Cassette Acceptor allele Rosa26{tm1.8(Non-EN-CFP)}). This ES cell line contains CFP (Cerulean) inserted into a Rosa26{LCA} allele by recombinase mediated cassette exchange. These cells were used to identifythe optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ngn3 raised in goat A polyclonal antibody raised in goat that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN-CFP-Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN.CFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains CFP (Cerulean) inserted into a Rosa26 LCA allele by recombinase mediated cassette exchange. These cells were used to identify the optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN.CFP.SV40.Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{CFP.SV40} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains CFP (Cerulean) inserted into a Rosa26 LCA allele by recombinase mediated cassette exchange. These cells were used to identify the optimal arrangement of regulatory elements for fluorescent protein expression from a single genomic copy. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN-GFP-Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN.GFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains eGFP inserted into the Rosa26 gene locus. These cells were used to identify an optimal combination of regulatory elements for fluorescent protein expression from a single genomic copy and can serve as a reference cell line for fluorescent cell sorting. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN-CFP-bGpA-Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{CFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains CFP (Cerulean) inserted into a Rosa26{LCA} allele by recombinase mediated cassette exchange. These cells were used to identify an optimal combination of regulatory elements for fluorescent protein expression from single a genomic copy. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(Ngn3.CFP)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{Ngn3-CFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse contains a bidirectional TetO-regulated fusion gene that has been inserted into a disabled Rosa26 locus. In one direction the tetO/CMV promoter drives expression of CFP (Cerulean). In the other direction it drives Ngn3. Mice containing this allelecan be used to drive the expression of Ngn3 under control of both doxycycline and either an rtTA or tTA transgene. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(Ngn3.CFP)Mgn} Vanderbilt University Medical Center Rosa26{tm1.4(MafA-Cherry)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{MafA-Cherry} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse contains a bidirectional Tet0-regulated fusion gene that has been inserted into adisabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives MafA expression. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm1.4(MafA-Cherry)Mgn} Vanderbilt University Medical Center Rosa26{R26-60-3XCNotch-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{(R26-60-CNotch-TA-Cerulean)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). The Rosa26 gene sequences from -228 to +81 were replaced with a repeat of alternating 32-bp C-elements and 31-bp Rbpj-binding sites (i.e.,C-R-C-R-C-R). The 31 base-pair region is derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc; uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh, MCB 16:952, 1996). The 32-bp C-element is from the Ela1 enhancer (Kruse et al., MCB 15:4385, 1995). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-60-AR8-TA-mCherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{R26-60-AR8-TA-mCherry} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -60 to +81 were replaced by an Activin response element (AR8) fused to a TATA-mCherry reporter Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-228-AR8-TA-mCherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{tm2.2(R26-228-AR8-TA-mCherry)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In these cells Rosa26 gene sequences from -228 to +81 were replaced by an Activin response element (AR8) fused to a TATA-mCherry reporter. These ESCs may be usefulto assess Activin, Nodal, and TGFbeta responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{R26-60-6XNotch-TA-Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26(R26-60-6XNotch-TA-Cerulean) using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). The binding site for Rbpj, the transcriptional mediator of Notch signaling was inserted into the Rosa 26 locus replacing nucleotides -60 to -228.The complete insert contains the Rbpj cis element in a series of six repeats. Each repeat includes a 31 base-pair region derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc). Uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh et al., MCB 16:952, 1996). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{-228.3XCNotch.TA.Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26(R26-228-3XCNotch-TA-Cerulean) using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). The Rosa26 gene sequences from -60 to -228 were replaced with a repeat of alternating 32-bp C-elements and 31-bp Rbpj-binding sites (i.e.,C-R-C-R-C-R). The 31 base-pair region is derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc; uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh, MCB 16:952, 1996). The 32-bp C-element is from the Ela1 enhancer (Kruse et al., MCB 15:4385, 1995). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{-228.6XNotch.TA.Cerulean} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26(Rbpj(R26-228-6XNotch-TA-Cerulean)) using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). The binding site for Rbpj, the transcriptional mediator of Notch signaling was inserted into the Rosa 26 locus replacing nucleotides -60 to -228.The complete insert contains the Rbpj cis element in a series of six repeats. Each repeat includes a 31 base-pair region derived from the CBF1/Rbpj binding region of the C-promoter of EBV (cataaattTTTTCCCACGgcgtgtttacacc). Uppercase letters are the consensus Rbpj binding sequence within the 31-bp element) (Hsieh et al., MCB 16:952, 1996). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Irx2 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Irx2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Arx raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Arx" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center ROSA26{(Nkx2.2.CFP)Suss} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{Nkx2.2/CFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In this strain both CFP and myc-tagged Nkx2.2 were inserted into a ROSA26 LCA allele under control of a bidirectional tetO/CMV promoter element. The mouse line will allow us to explore the contribution of Nkx2.2 to progenitor cell population competency by allowing for the manipulation of Nkx2.2 gene expression within the developing pancreas. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler ROSA26{(Nkx2.2.CFP)Suss} Vanderbilt University Medical Center Polyclonal mouse mArx raised in guinea pig A polyclonal antibody raised in guinea pig that targets the mouse "mArx" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human C-peptide raised in rat A monoclonal antibody raised in rat that targets the human "C-peptide" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(-60.IBRE4.TACerulean)Hri} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{tm2.1(R26-60-IBRE4-TA-Cerulean)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse contains aBMP reporter element withina modified ROSA26 promoter followed by a CFP reporter. This mouse is identical to the -228 variant except for the amount of ROSA26 DNA. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(-60.IBRE4.TACerulean)Hri} Vanderbilt University Medical Center Ptf1a{rtTA} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{rtTA} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). These mESCs contain the reverse tetracycline transactivator (rtTA) under control of the Ptf1a regulatory sequences. rtTA is a transcriptional activator, which can inducibly (in the presence of the tetracycline analog doxycycline) activate the genes under Tet-O activator/promoter element. The rtTA sequences were introduced in to Ptf1a[LCA] by Recombinase-Mediated Cassette Exchange using standard methods.These cells were made in order togenerate a mouse line that express rtTA under control of the Ptf1a gene locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{Nepn.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Nepn-Cherry using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line was generated by RMCE usingthe Rosa26.LCA allele. To create a Nepn reporter allele, a 9kb promoter region of the Nepn gene is inserted upstream of the red fluorescent protein mCherry-pA sequence. Since Nepn is expressed during endoderm development, this cell line can be used for visualisation and sorting of endodermal cell populations. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{EN.YFP.bGsplicepA.Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{YFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). These mESCs contain yellow fluorescent protein (YFP, Citrine) under control of the ROSA26 gene locus. They were made as part of a study to quantify variables that affect fluorescent protein expression levels in mESCs. TheYFP sequences are flanked by a translational enhancer and beta-globin splice/polyA sequences. The cassette containing the YFP was inserted intoa ROSA26 LCA allel using Recombinase-Mediated Cassette Exchange. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{Apple.bGsplicepA.Neo} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN.Apple.bGspliceA.neo} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). To better quantify protein expression levels via confocal microscopy, flow sorting and western blot analysis this cell line contains a 4kb R26 promoter element, Apple (a red fluorescent protein) with an upstream enhancer element, a splicing beta-globin polyA tail and the neomycin gene as a selection agent. Through Recombinase-Mediated Cassette Exchange this construct was inserted into the Rosa26 locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{Ngn3.Mafa} mouse ESC line A mouse ESC line in which part or all of the targeted gene, Gt(ROSA)26Sor gene trap ROSA 26, Philippe Soriano [ Mus musculus ] (NCBI Gene ID: 14910) is replaced to create Rosa26 LCA using the Loxed Cassette Acceptor allele ). Recombinase mediated cassette exchange was performed to introduce a bidirectional tetO-regulated cassette containing coding sequences for transcription factors MafA and Ngn3 into Rosa26 LCA mESCs. ThismESC line can be used to generate mice that will simultaneously overexpress MafA and Ngn3 using tet-inducible activation with rtTA. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{tm2Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{EN-Cherry-Neo} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line expresses mCherry, a red fluorescent protein, under control of the endogenous ROSA26 gene locus. This mouse was generated as part of a study to identify the optimal combination of regulatory elements for fluorescent protein expression from a single gene copy. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm2Mgn} Vanderbilt University Medical Center Ptf1a{tm2(rtTA)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{tm1.2(rtTA)Mgn} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). This mouse line expresses the reverse tetracycline transactivator (rtTA) under control of the Ptf1a regulatory sequences. This mouseisuseful for activating expression of various TetO-regulated genes in sites where Ptf1a is normally expressed. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{tm2(rtTA)Mgn} Vanderbilt University Medical Center Rosa26{(Nepn.Cherry)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26[Nepn.Cherry] using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). Nephrocan (Nepn) has been shown to be expressed specifically during early endoderm development. A Nepn promoter driven reporter (Cherry) has been inserted in the Rosa26LCA locus by recombinase mediated cassette exchange. This mouse line will be useful in visualizing whether Nepn can be used as a specific DE marker for beta-cell development. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(Nepn.Cherry)Mgn} Vanderbilt University Medical Center Rosa26{mIre1.WT.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, Gt(ROSA)26Sor gene trap ROSA 26, Philippe Soriano [ Mus musculus ] (NCBI Gene ID: 14910) is replaced to create Rosa26 LCA using the Loxed Cassette Acceptor allele Rosa26{tm1.1(mlre1.WT.Cherry)FerozPapa}). This mESC line contains a bidirectional Tet0-regulated fusion gene that has been inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives the wild type form of mlre1. mlre1 is an endoplasmic reticulum (ER) membrane kinase response to unfolded protein response (UPR). Activated mlre1 endonucleases leads to the splicing of XBP-1 (a transcription factor which is upregulated in times of ER stress) which transcriptionally increases the expression of ER chaperones and alleviates UPR. This cell line should be useful for studying the role of mlre1 in the response to ER stress. By generating mice with this allele Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{mIre1.IG.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, Gt(ROSA)26Sor gene trap ROSA 26, Philippe Soriano [ Mus musculus ] (NCBI Gene ID: 14910) is replaced to create Rosa26 LCA using the Loxed Cassette Acceptor allele Rosa26{tm1.1(mlre1.IG.Cherry)FerozPapa}). This mESC line contains a bidirectional Tet0-regulated fusion gene that has been inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives a mutant version of mlre1. mlre1 is an endoplasmic reticulum (ER) membrane kinase response to unfolded protein response (UPR). Activated mlre1 endonucleases leads to the splicing of XBP-1 (a transcription factor which is upregulated in times of ER stress) which transcriptionally increases the expression of ER chaperones and alleviates UPR. This cell line should be useful for studying the role of mlre1 in the response to ER stress. By generating mice with this allele t Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(mlre1.WT.Cherry)Fpa} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{mlre1.WT-Cherry} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains a bidirectional Tet0-regulated fusion gene that has been inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives a wild type mlre1. mlre1 is an endoplasmic reticulum (ER) membrane kinase response to unfolded protein response (UPR). Activated mlre1 endonucleases leads to the splicing of XBP-1 (a transcription factor which is upregulated in times of ER stress) which transcriptionally increases the expression of ER chaperones and alleviates UPR. These mice may be useful for studying the role of mlre1 in the response to ER stress in the setting of diet induced obesity and insulin resistance. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(mlre1.WT.Cherry)Fpa} Vanderbilt University Medical Center Rosa26{(mlre1.IG.Cherry)Fpa} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{mlre1.IG-Cherry} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains a bidirectional Tet0-regulated fusion gene that has been inserted into a disabled Rosa26 loxed cassette acceptor allele by RMCE. In one direction the tetO/CMV promoter drives expression of a red fluorescent protein (Cherry) while in the other direction it drives a mutant mlre1. mlre1 is an endoplasmic reticulum (ER) membrane kinase response to unfolded protein response (UPR). Activated mlre1 endonucleases leads to the splicing of XBP-1 (a transcription factor which is upregulated in times of ER stress) which transcriptionally increases the expression of ER chaperones and alleviates UPR. These mice may be usefulfor studying the role of mlre1 in the response to ER stress in the setting of diet induced obesity and insulin resistance. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(mlre1.IG.Cherry)Fpa} Vanderbilt University Medical Center Rosa26{(R26-228-IBRE4-TA-Cerulean)Hri} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{R26-228-IBRE4-TA-Cerulean} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). These mice were generated by performing recombinase mediated cassette exchange on the Rosa26 LCA. The sequence from -228 to +81 of the Rosa26 gene was replaced with a BMP responsive element (IBRE4) fused to a TATA-Cerulean (CFP) reporter. These mice may be useful in visualizing BMP responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(R26-228-IBRE4-TA-Cerulean)Hri} Vanderbilt University Medical Center Rosa26{Sox9.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Sox9/mCherry using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES line contains bidirectional TetO-regulated genes inserted into the Rosa26.LCA allele by RMCE. In one direction the TetO/CMV promoter drives the expression of transcription factor Sox9. In the other direction it drives the expression of red fluorescent protein mCherry. This ES cell line can be used to simultaneously over-express Sox9 and mCherry upon administration of doxycyline when the effector protein rtTA is expressed. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(TetO.Sox9)Msan} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Sox9 using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. In one direction, the TetO/CMV promoter drives the expression of the transcription factor Sox9. In the other direction, it drives the expression of the red fluorescent protein mCherry. In this mouse line, when the effector protein rtTA is expressed, Sox9 and mCherry will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(TetO.Sox9)Msan} Vanderbilt University Medical Center Rosa26{TetO.NRSF} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create NRSF using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This cell line contains bidirectional TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. In one direction, the TetO/CMV promoter drives expression of the transcription factor NRSF/REST. In the other direction, it drives the expression of the red fluorescent protein mCherry. In this cell line, when the effector protein rtTA is expressed, NRSF and mCherry are simultaneously over-expressed upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{tdTOM} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{td.TOM} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). Recombinase mediated cassette exchange was performed using the Ptf1aLCA. This cell line expresses both tdTOM (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of ptf1a in the adult pancreas. These cells will be useful in a variety of applications such as FACS sorting of ptf1a expressing cells, chromatin immunoprecipitation and proteomic analyses. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human TSPAN7 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "TSPAN7" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human FXYD2 raised in mouse A monoclonal antibody raised in mouse that targets the human "FXYD2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Sez6l2 raised in rabbit A polyclonal antibody raised in rabbit that targets the mouse "Sez6l2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Lrp11 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Lrp11" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Onecut1 raised in guinea pig A polyclonal antibody raised in guinea pig that targets the mouse "Onecut1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{tm3Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create CFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line was made by RMCE in the Rosa26[LCA] allele and has a cyan fluorescent protein (CFP, Cerulean) expressed under the control of the ROSA26 promoter. The CFP sequence is preceded by a translational enhancer and followed by intron-containing rabbit beta-globin polyA sequences. This mouse has ubiquitously expressed CFP protein and can be used for transplantation and other experiments where tracing of source cells is required. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm3Mgn} Vanderbilt University Medical Center Rosa26{tm4Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create YFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line was made by RMCE in the Rosa26[LCA] allele and has yellow fluorescent protein (YFP, Citrine) expressed under control of the ROSA26 promoter. The YFP sequence is preceded by a translational enhancer and followed by intron-containing rabbit beta-globin polyA sequences. This mouse has ubiquitously expressed YFP protein and can be used for transplantation and other experiments where tracing of source cells is required. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{tm4Mgn} Vanderbilt University Medical Center Rosa26{Setd5.GFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Setd5/GFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line was generated by RMCE in the Rosa26{LCA} allele. A coding sequence for green fluorescent protein (GFP) was inserted into the first exon of the Setd5 gene. This exon is located upstream of the Rosa26 transcribed sequence and is transcribed in an opposite orientation. The GFP-polyA cassette, transcribed from the Setd5 promoter, knocks out Setd5 transcription and provides a reporter for Setd5 gene expression. The purpose of this cell line is to study the molecular function of the Setd5 gene. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{(H2B.Apple)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{H2B.Apple} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). This mouse contains a coding sequence for the mApple-H2B fusion protein in order to tag cells expressing Pdx1 with a red fluorescent protein. The generation of a Pdx-1 red fluorescent marker will enable the isolation of pancreatic lineage progenitors by FACS. The presence of the H2B sequences may also be useful in allowing the plane of cell division to be analyzed. Pdx1{(H2B.Apple)Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ngn3{HA.LCA} mouse ESC line A mouse ESC line in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Cassette Acceptor) to (MGI ID: ). This ES cell line was made by gene targeting and designed to express an HA-tagged version of the transcription factor Ngn3. The Lox71 and Lox2272 sites were inserted flanking the Ngn3 coding sequence. Within the flanked region an HA-tag was placed at the N-terminus of the Ngn3 protein. This allele can be used to study chromatin and protein-protein interactions of Ngn3. Additionally, the Lox71 and Lox2272 sites allow for manipulations of the flanked region by RMCE. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Foxm1{LCA} mouse ESC line A mouse ESC line in which the targeted gene, Forkhead box M1 (NCBI Gene ID: 14235), has been modified (allele type: Gene Replacement) to (MGI ID: ). This ES cell line was made by gene targeting and is designed to serve as a cassette acceptor allele at the Foxm1 locus. The portion of genomic DNA containing exons 2-4 of Foxm1 was flanked with Lox71 and Lox2272 sites and replaced with a selection marker. This line allows for modification of the flanked sequences through RMCE. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{Pdx1.YFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Pdx1/YFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains bidirectional TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. In one direction, the tetO/CMV promoter will drive expression of Pdx1. In the other direction, it will drive the expression of yellow fluorescent protein (YFP). In this cell line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(Pdx1.YFP)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Pdx1/YFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26[LCA] allele by RMCE. In one direction, the TetO/CMV promoter will drive expression of Pdx1. In the other direction, it will drive the expression of yellow fluorescent protein (YFP). In this mouse line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(Pdx1.YFP)Mgn} Vanderbilt University Medical Center Insm1{Pdx1.YFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, insulinoma-associated 1 (NCBI Gene ID: 53626) is replaced to create Pdx1/YFP using the Loxed Cassette Acceptor allele Insm1{tm1(LCA)}). This ES cell line contains bidirectional TetO-regulated genes inserted into the Insm1{LCA} allele by RMCE. In one direction, the TetO/CMV promoter drives the expression of the transcription factor Pdx1. In the other direction, it drives the expression of yellow fluorescent protein (YFP). In this cell line, when the effector protein rtTA is expressed, Pdx1 and YFP will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{3TF.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create mCherry/MafA/Pdx1/Ngn3 using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line contains bidirectional TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. In one direction, the TetO/CMV promoter drives the expression of a polycistronic mRNA with transcription factors MafA, Pdx1, and Ngn3 linked by 2A peptide cleavage sequences. In the other direction, it drives the expression of the red fluorescent protein mCherry. In this cell line, when the effector protein rtTA is expressed, all three transcription factors and mCherry will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(3TF.Cherry)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create mCherry/MafA/Pdx1/Ngn3 using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26[LCA] by RMCE. In one direction, the TetO/CMV promoter drives expression of a polycistronic mRNA with transcription factors MafA, Pdx1, and Ngn3 linked by 2A peptide cleavage sequences. In the other direction, it drives the expression of red fluorescent protein mCherry. In this mouse line, when the effector protein rtTA is expressed, all three transcription factors and mCherry will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(3TF.Cherry)Mgn} Vanderbilt University Medical Center Pdx1{AIVd} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{aIVd} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this ES cell line, Area IV of the Pdx1 enhancer (-2168/-1913 relative to the primary transcription start site) was deleted using RMCE in the Pdx1{LCA} allele. This cell line allows for assessment of the role of these DNA sequences in the regulation and function of Pdx1. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{(AIVd)Rwst} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{area IV deletion} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this mouse line, Area IV of the Pdx1 enhancer (-2168/-1913 relative to the primary transcription start site) was deleted using RMCE in the Pdx1[LCA] allele. This mouse line allows for assessment of the role of these DNA sequences in the regulation and function of Pdx1. Pdx1{(AIVd)Rwst} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{cCre-Knock-in} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{Cre} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this ES cell line, the sequence for one of the two bi-partite Cre molecules (cCre) and the IRES sequence were knocked into a site directly upstream of the Nkx2.2 start codon using RMCE in the Nkx2.2{LCA} allele. The presence of the IRES sequence allows for the transcription of cCre and Nkx2.2 from the native Nkx2.2 locus at the same time. This results in the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{(Cre-Knock-in)} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{Cre-knock-in)} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this mouse line, one of the two bi-partite Cre molecules, cCre, together with the IRES sequence was knocked into the site directly upstream of the Nkx2.2 start codon using RMCE in the Nkx2.2[LCA] allele. The presence of the IRES sequence allows for transcription of cCre and Nkx2.2 from the native Nkx2.2 locus at the same time. This mouse line allows the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Nkx2.2{(Cre-Knock-in)} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{3XHA.Nkx2.2} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{3XHA-Nkx2.2} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this cell line RMCE in the Nkx2.2.LCA allele was used to introduce a triple HA tag in frame at the N-terminus of the transcription factor Nkx2.2. This allows for efficient immunoprecipitation of HA-tagged Nkx2.2 protein from mouse tissues to perform ChIP and protein-protein interaction studies. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{(3XHA.Nkx2.2)Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{3XHA-Nkx2.2} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this mouse line, RMCE in the Nkx2.2{LCA} allele was used to introduce a triple HA tag in frame at the N-terminus of the transcription factor Nkx2.2. This allows for efficient immunoprecipitation of HA-tagged Nkx2.2 protein from mouse tissues to perform ChIP and protein-protein interaction studies. Nkx2.2{(3XHA.Nkx2.2)Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{Cre.EGFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{Cre-EGFP} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this ES cell line, a coding portion of the transcription factor Nkx2.2 was replaced with a Cre-EGFP cassette, in frame to the Nkx2.2 ATG. This allows for sites of Nkx2.2 gene expression to be identified in mice using direct immunofluorescence, FACS purification of Nkx2.2 expressing cells, and lineage tracing using Nkx2.2 driven Cre expression. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{(Cre-ER)} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1/cre-ER using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this mouse line, the first exon of the transcription factor Pdx1 was replaced with the coding sequence for a Cre-ER fusion protein using RMCE in the Pdx1[LCA] allele. Pdx1-promoter driven Cre-ER expressing mice enable tamoxifen-inducible Cre-LoxP manipulation of pancreatic lineage cells. Pdx1{(Cre-ER)} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Cherry using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this ES cell, 1.956 kb of the native Nkx2.2 gene, containing a portion of exon 1 (all but the first 214 bp) and all of exon 2, was replaced by the mCherry cassette in frame to the Nkx2.2 ATG. This allows for sites of Nkx2.2 gene expression in the mouse to be identified using direct immunofluorescence and FACS purification of Nkx2.2 expressing cells. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal enterobacteria phage p1 Cre raised in guinea pig A polyclonal antibody raised in guinea pig that targets the enterobacteria phage p1 "Cre" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pax4{HA.LCA} mouse ESC line A mouse ESC line in which the targeted gene, paired box 4 (NCBI Gene ID: 18506), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Pax4{tm1(LCA)Ggu}). This ES cell line is made by gene targeting and is designed to express an HA-tagged version of the transcription factor Pax4. Lox71 and Lox2272 sites are inserted, flanking the last coding exon of the Pax4 gene. In the flanked region, an HA-tag was placed at the C-terminus of the PAX4 protein. This allele can be used to study chromatin and protein-protein interactions of PAX4. Additionally, the Lox71 and Lox2272 sites allow for future manipulations of the flanked region by RMCE. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human CDCP1 raised in goat A polyclonal antibody raised in goat that targets the human "CDCP1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human CD-200 raised in goat A polyclonal antibody raised in goat that targets the human "CD-200" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Coagulation factor 3(rhF3) raised in goat A polyclonal antibody raised in goat that targets the human "Coagulation factor 3(rhF3)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Ngn3 raised in sheep A polyclonal antibody raised in sheep that targets the human "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Pax4 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "Pax4" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Pax4 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "Pax4" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal mouse Pax4 raised in mouse A monoclonal antibody raised in mouse that targets the mouse "Pax4" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human CD142 raised in mouse A monoclonal antibody raised in mouse that targets the human "CD142" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Beta cell, IC2 raised in rat A monoclonal antibody raised in rat that targets the rat "Beta cell, IC2" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human CD-200 raised in goat A polyclonal antibody raised in goat that targets the human "CD-200" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl}/SzJ mouse strain A mouse strain in which the targeted gene, protein kinase, DNA activated, catalytic polypeptide (NCBI Gene ID: 19090), has been modified (allele type: Other) to severe combined immunodeficiency (MGI ID: Prkdc{scid}). The NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice, commonly known as NOD scid gamma (NSG), do not express the Prkdc gene nor the X-linked Il2rg gene. NSG mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Histological examination of lymphoid tissues reveals absence of lymphoid cells and some cystic structures in the thymus, an absence of follicles in the spleen and markedly diminished celluarity of lymph nodes. NSG mice are deficient in mature lymphocytes, serum Ig is not detectable and natural killer (NK) cell cytotoxic activity is extremely low. These mice are resistant to lymphoma development even after sublethal irradiation treatment. These mutant mice have been shown to readily support engraftment of human CD34+ hematopoietic stem cells and represent a superior, long-lived model suitable for studies employing xenotransplantation strategies. Please note that the NSG carries the true null interleukin-2 receptor gamma chain mutation and should not be confused with other strains that express a truncated interleukin-2 receptor gamma chain as described in: "Modulation of hematopoiesis in mice with a truncated mutant of the interleukin-2 receptor gamma chain". NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl}/SzJ Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Rag1{tm1Mom}Ins2{Akita}Il2rg{tm1Wjl}/SzJ mouse strain A mouse strain in which the targeted gene, insulin II (NCBI Gene ID: 16334), has been modified (allele type: Other) to Akita (MGI ID: Ins2{Akita}). NRG-Akita mice, which are homozygous for the Rag1tm1Mom and the Il2rgtm1Wjl alleles (males are hemizygous for the X-linked Il2rgtm1Wjl allele) and heterozygous for the Ins2Akita allele, develop spontaneous hyperglycermia. No mature T, B or NK cells are detected in flow cytometric analysis of splenocytes from NRG-Akita mutant mice. Granulocyte and macrophage populations are similar to those seen in NRG mice. NRG-Akita mice develop hyperglycemia between 3 and 5 weeks of age. Histological examination at 3 weeks of age reveals normal pancreas morphology, and routine insulin and glucagon staining. By approximately 32 weeks of age, NRG-Akita mice display disorganized, condensed pancreatic islet architecture, with loss of insulin-positive cells. Euglycemia is restored by subrenal transplantation of mouse or human islets or intrapancreatic transplantation of dissociated mouse islet cells. NRG-Akita mice engrafted with human hematopoietic stem cells (HSC) develop humanized immune systems. Approximately 60% of the human HSC engrafted NRG-Akita mice reject human islet allografts. NOD.Cg-Rag1{tm1Mom}Ins2{Akita}Il2rg{tm1Wjl}/SzJ Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{Cre.ER} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1/cre-ER using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this cell line, the first exon of the transcription factor Pdx1 was replaced with the coding sequence for a Cre-ER fusion protein using RMCE in the Pdx1{LCA} allele. Pdx1-promoter driven Cre-ER expressing mice enable tamoxifen-inducible Cre-LoxP manipulation of pancreatic lineage cells. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ngn3{(HA.LCA)} mouse strain A mouse strain in which the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925), has been modified (allele type: Cassette Acceptor) to (MGI ID: ). This mouse line was made by gene targeting and designed to express an HA-tagged version of the transcription factor Ngn3. The Lox71 and Lox2272 sites were inserted flanking the Ngn3 coding sequence. Within the flanked region an HA-tag was placed at the N-terminus of the Ngn3 protein. This allele can be used to study chromatin and protein-protein interactions of Ngn3. Additionally, the Lox71 and Lox2272 sites allow for manipulations of the flanked region by RMCE. Ngn3{(HA.LCA)} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pax4{(HA.LCA)Ggu} mouse strain A mouse strain in which the targeted gene, paired box 4 (NCBI Gene ID: 18506), has been modified (allele type: Cassette Acceptor) to targeted mutation 1 (MGI ID: Pax4{tm1(LCA)Ggu}). This mouse line was made by gene targeting and designed to express an HA-tagged version of the transcription factor Pax4. The Lox71 and Lox2272 sites were inserted flanking the last coding exon of the Pax4 gene. Within the flanked region an HA-tag was placed at the C-terminus of the PAX4 protein. This allele can be used to study chromatin and protein-protein interactions of PAX4. Additionally, the Lox71 and Lox2272 sites allow for manipulations of the flanked region by RMCE in the future. Pax4{(HA.LCA)Ggu} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(Setd5.GFP)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Setd5/GFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line was generated by RMCE in the Rosa26[LCA] allele. A coding sequence for green fluorescent protein (GFP) was inserted into the first exon of the Setd5 gene. This exon is located upstream of the Rosa26 transcribed sequence and is transcribed in an opposite orientation. The GFP-polyA cassette, transcribed from the Setd5 promoter, knocks out Setd5 transcription and provides a reporter for Setd5 gene expression. The purpose of this mouse line is to study the molecular function of the Setd5 gene. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(Setd5.GFP)Mgn} Vanderbilt University Medical Center Rosa26{228.TOP.CFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create 7xTcf/Lef binding site using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line was generated by RMCE in the Rosa26{LCA} allele. The Rosa26 gene sequence (from -228 to +81) was replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. This ES cell line may be useful for assessing canonical Wnt responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ptf1a{(tdTomato)} mouse strain A mouse strain in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{td.TOM} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). This mouse cell line was generated by RMCE in the Ptf1a[LCA] allele. Pancreata of this mouse line express both td.Tomato (a red fluorescent protein) and an N-terminal epitope tagged Strep/Flag (NSF) fusion of the transcription factor Ptf1a. This line may be used in a variety of applications such as FACS sorting of Ptf1a expressing cells, chromatin immunoprecipitation, and proteomic analyses. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Ptf1a{(tdTomato)} Vanderbilt University Medical Center Nkx2.2{(Cre.EGFP)Suss} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{Cre-EGFP} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this mouse, a coding portion of the transcription factor Nkx2.2 was replaced with a Cre-EGFP cassette, in frame to the Nkx2.2 ATG. This allows for sites of Nkx2.2 gene expression to be identified in mice using direct immunofluorescence, FACS purification of Nkx2.2 expressing cells, and lineage tracing using Nkx2.2 driven Cre expression. Nkx2.2{(Cre.EGFP)Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{(TetO.NRSF)Msan} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create NRSF using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line contains bidirectional TetO-regulated genes inserted into the Rosa26[LCA] allele by RMCE. In one direction, the TetO/CMV promoter drives the expression of the transcription factor NRSF/REST. In the other direction, it drives the expression of red fluorescent protein mCherry. In this mouse line, when the effector protein rtTA is expressed, NRSF and mCherry will be simultaneously over-expressed upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{(TetO.NRSF)Msan} Vanderbilt University Medical Center Gt(ROSA)26Sor{tm2.1(mix1b-mCherry)Mgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{(R26-228-AR8-TA-mCherry)} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse line was generated by RMCE in the Rosa26[LCA] allele. In this mouse line, the Rosa26 gene sequence (-228 to +81) was replaced by the Foxh1/Smad2-dependent, Mix2-derived Activin response element (AR8) fused to a TATA-mCherry reporter. These mice may be useful to assess Activin, Nodal, and TGFbeta responsiveness. Gt(ROSA)26Sor{tm2.1(mix1b-mCherry)Mgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nepn{Cherry} mouse ESC line A mouse ESC line in which the targeted gene, Nephrocan (NCBI Gene ID: 66650), has been modified (allele type: Global Null) to (MGI ID: Neph(mCherry)). This ES cell line was made by gene targeting and was designed to express red fluorescent protein (mCherry) from the Nepn promoter thereby disrupting the expression of Nepn. The FP coding sequence was inserted dowstream of the Nepn start codon, in front of the Nepn first exon. This cell line is used to study the function and expression of Nepn. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ins2{Apple.LCA} mouse ESC line A mouse ESC line in which the targeted gene, Insulin 2 (NCBI Gene ID: 20671), has been modified (allele type: Cassette Acceptor) to (MGI ID: Ins2{(apple.LCA)}). This ES cell line was made by gene targeting and designed to express a H2B-Apple fusion protein (nuclear red fluorescent protein) from the Ins2 promoter. The FP coding sequence is downsteam of the Ins2 start codon (replacing exon 2 of Ins2) and is flanked by Lox66/Lox2272 sites to allow for future manipulations of the flanked region by RMCE. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{rTTA} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{rTTA} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this cell line, thefirst exon of Pdx1 was replaced with the coding sequence for rTTA protein, using RMCE in the Pdx1{LCA} allele. Generation of Pdx1-promoter driven rTTA expressing mice will enable tetracycline-inducible manipulation of pancreatic lineage cells. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{Cre} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1/cre using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this cell line, thefirst exon of Pdx1 was replaced with the coding sequence for Cre-recombinase using RMCE in the Pdx1{LCA} allele. Generation of Pdx1-promoter driven Cre-expressing cells will enable Cre-LoxP manipulation of pancreatic lineage cells. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{228.3TF.GFP.Cre} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create 3TF.GFP-Cre using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This cell line contains TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. The TetO/miniCMV promoter was placed (at -228) upstream of the putative ROSA26 transcription start site and drives the expression of a polycistronic mRNA with transcription factors MafA, Pdx1, and Ngn3 together with a GFP-Cre fusion protein. The sequences are linked by 2A peptide cleavage sequences. In this cell line, when the effector protein rtTA is expressed, all three transcription factors and GFP-Cre will be over-expressed simultaneously upon administration of doxycyline. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{mIre1.N906A.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{mIre1.N906A.Cherry} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES line contains bidirectional TetO-regulated genes inserted into the Rosa26{LCA} allele by RMCE. In one direction the TetO/CMV promoter drives the expression of point mutant(N906A) of IRE1, an ER transmenbrane kinase response to unfolded protein response (UPR). In the other direction it drives the expression of red fluorescent protein mCherry. This ES cell line can be used to simultaneously over-express Ire1(N906A) and mCherry upon administration of doxycyline when the effector protein rtTA is expressed. This line can be used to study the roles of IRE1 in the response to ER stress, diet induced obesity and insulin resistance. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human HIP-14 ZDHHC17 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "HIP-14 ZDHHC17" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ghrl{tm1(Cre-eGFP)Suss} mouse strain A mouse strain in which part or all of the targeted gene, Ghrelin (NCBI Gene ID: 58991) is replaced to create Ghrl Cre-eGFP CEV using the Loxed Cassette Acceptor allele Ghrl{LCA}). Ghrl{Cre-eGGP} mice contain a Cre-eGFP fusion protein which replaces the Ghrelin coding sequence. These mice expressc Cre-eGFP phusion protein under control of the Ghrelin gene locus. The Ghrl{Cre-eGGP} mice may be used to track ghrelin-expressing cells progeny,or to conditionally inactivate genes in ghrelin-expressing cells. Ghrl{tm1(Cre-eGFP)Suss} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Cd31 (PECAM1) raised in mouse A monoclonal antibody raised in mouse that targets the human "Cd31 (PECAM1)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal cow GFAP raised in rabbit A polyclonal antibody raised in rabbit that targets the cow "GFAP" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Glucagon (GCG) raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Glucagon (GCG)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Glucagon (GCG) raised in guinea pig A polyclonal antibody raised in guinea pig that targets the human "Glucagon (GCG)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal pig Insulin raised in guinea pig A polyclonal antibody raised in guinea pig that targets the pig "Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Ki67 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Ki67" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human MafA raised in rabbit A polyclonal antibody raised in rabbit that targets the human "MafA" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human MafB raised in rabbit A polyclonal antibody raised in rabbit that targets the human "MafB" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Pancreatic Polypeptide (PPY) raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Pancreatic Polypeptide (PPY)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Somatostatin (Sst) raised in sheep A polyclonal antibody raised in sheep that targets the human "Somatostatin (Sst)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal rat Tuj1 (TUBB3) raised in rabbit A monoclonal antibody raised in rabbit that targets the rat "Tuj1 (TUBB3)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat VEGFA raised in goat A polyclonal antibody raised in goat that targets the rat "VEGFA" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Foxa2 (HNF3b) raised in goat A polyclonal antibody raised in goat that targets the mouse "Foxa2 (HNF3b)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Muc1 raised in hamster (armenian) A monoclonal antibody raised in hamster (armenian) that targets the human "Muc1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human HNF-1β raised in goat A polyclonal antibody raised in goat that targets the human "HNF-1β" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Sox9 raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Sox9" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human CDCP1 raised in goat A polyclonal antibody raised in goat that targets the human "CDCP1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human α-Amylase (AMY2A) raised in rabbit A polyclonal antibody raised in rabbit that targets the human "α-Amylase (AMY2A)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal cow Insulin raised in guinea pig A polyclonal antibody raised in guinea pig that targets the cow "Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Insulin raised in guinea pig A polyclonal antibody raised in guinea pig that targets the human "Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Insulin raised in goat A polyclonal antibody raised in goat that targets the human "Insulin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Glucagon raised in rabbit A polyclonal antibody raised in rabbit that targets the human "Glucagon" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal human Somatostatin (Sst) raised in goat A polyclonal antibody raised in goat that targets the human "Somatostatin (Sst)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal rat Synaptophysin (SYP) raised in rabbit A polyclonal antibody raised in rabbit that targets the rat "Synaptophysin (SYP)" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Ngn3{T2A.nCre} mouse ESC line A mouse ESC line in which part or all of the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925) is replaced to create Ngn3{T2A.nCre} using the Loxed Cassette Acceptor allele ). In this mouse ES cell line, one of the two bi-partite Cre molecules, nCre, was knocked in upstream of Ngn3 coding sequence by RMCE in Ngn3.HA.LCA ES cell line. nCre sequence is expressed from policistronic mRNA linked by 2a peptide to Ngn3 coding sequence allowing for simultanious expression of both proteins from Ngn3 promoter. This mouse line, in combination with Nkx2.2-cCre mouse line, allows to label pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{T2A.cCre} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{T2A.cCre} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this line, one of the two bi-partite Cre molecules, cCre, is inserted downstream of Nkx2.2 coding sequence fused with 2a peptide using RMCE in the Nkx2.2{LCA}allele. The presence of the T2A sequence allows for transcription of Nkx2.2 and cCre from the native Nkx2.2 locus at the same time. Mouse lines obtained from these ES cells allow the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Neurog3{Cre-ER.BAC} mouse ESC line A mouse ESC line containing a transgene in which Cre (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). This line has a single copy of Neurog3.Cre-ER.LCA BAC transgene inserted in a genome. The transgene is designed to express Cre-ER under control of Neurog 3 promoter and will allow inducible tracking or gene inactivation in the Ngn3-LOW and Ngn3-HIGH populations of pre-endocrine cells. Additionally, the lox66 and lox2272 sites will allow the BAC transgene to serve as a loxed cassette acceptor (LCA) in ES cells for future recombinase-mediated cassette exchange (RMCE). Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{CreMgn} mouse strain A mouse strain in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{Cre} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this mouse strain, the first exon of Pdx1 is replaced with coding sequence for Cre recombinase using RMCE in the Pdx1{LCA} allele. Generation of Pdx-promoter driven Cre expressing mice will enable Cre-LoxP manipulation of pancreatic lineage cells. Pdx1{CreMgn} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{228.TOP.CFPHri} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26{228.TOP.CFP} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). In this mouse strain the Rosa26 gene sequence from -228 to +81 was replaced by a Wnt response element (7xTcf/Lef binding site) fused to a TATA-Cerulean (CFP) reporter. These mice may be useful in assessing Canonical Wnt responsiveness. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{228.TOP.CFPHri} Vanderbilt University Medical Center Neurog3{RG.BAC} mouse ESC line A mouse ESC line containing a transgene in which RG (transgene) is driven by the Neurog3 promoter (NCBI Gene ID: 11925). This line has a single copy of Neurog3.RG.LCA BAC transgene inserted in the genome. The transgene is designed to express an H2B-mCherry fusion protein in the nucleus and a membrane anchored EGFP (via GPI), both under the control of the Neurog3 promoter. This will allow for live-cell imaging of mitotic nuclear/chromosome dynamics and membrane protrusive behavior in endocrine progenitors. Additionally, Lox71 and Lox2272 sites flanking the transgene will allow for manipulations by RMCE in the future. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{228.3TF.GFP-CreMgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create ROSA26{228.3TF.GFP-Cre} using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse strain contains TetO-regulated genes inserted into the Rosa 26{LCA} allele by RMCE. TetO/miniCMV promoter is placed at -228 position upstream of putative ROSA26 transcription start site and drives the expression of a polycistronic mRNA with transcription factors MafA, Pdx1, and Ngn3 together with GFP-Cre fusion protein linked by 2A peptide cleavage sequences. These mice will be able to simultaneously over-express all three transcription factors and GFP-Cre upon administration of doxycyline when the effector protein rtTA is expressed. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{228.3TF.GFP-CreMgn} Vanderbilt University Medical Center Ngn3{T2A.nCreGgu} mouse strain A mouse strain in which part or all of the targeted gene, Neurogenin 3 (NCBI Gene ID: 11925) is replaced to create Ngn3{T2A.nCre} using the Loxed Cassette Acceptor allele ). In this mouse line, one of the two bi-partite Cre molecules, nCre, was knocked in upstream of Ngn3 coding sequence by RMCE in Ngn3{HA.LCA} ES cell line. nCre sequence is expressed from policistronic mRNA linked by 2a peptide to Ngn3 coding sequence allowing for simultanious expression of both proteins from the Ngn3 promoter. This mouse line, in combination with Nkx2.2{cCre} mouse line, allows for the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Ngn3{T2A.nCreGgu} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Pdx1{H2B.Neptune} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreatic and duodenal homeobox 1 (NCBI Gene ID: 18609) is replaced to create Pdx1{H2B.Neptune} using the Loxed Cassette Acceptor allele Pdx1{tm1(LCA)}). In this line, the first exon of Pdx1 is replaced with coding sequence for mNeptune (a far red fluorescent protein) using RMCE in the Pdx1 LCA allele. Generation of a Pdx-promoter driven mNeptune fluorescent allele will enable the isolation of pancreatic lineage cells by FACS and broaden the possibilities for combinatorial sorting with other fluorescent alleles. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Sst.rTTA.LCA mouse ESC line A mouse ESC line in which the targeted gene, Somatostatin (NCBI Gene ID: 20604), has been modified (allele type: Cassette Acceptor) to Sst.rTTA loxed cassette acceptor (MGI ID: Sst.rTTA{LCA}). This allelle is designed to express a tet-inducible reverse-transactivator under control of the somatostatin promoter. Lox66 and Lox2272 sites are inserted flanking 500bp of Sst promoter region, Sst exons I and II and Sst poly A site. The rTTA-beta-globin poly A cassete replaces Sst coding exons (I and II). The allele can be used to drive tet-inducible expression in somatostatin expressing cells (Delta-cells). Additionally, the Lox66 and Lox2272 sites allow for manipulations of the flanked region by RMCE in the future. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Foxa2{LCA} mouse ESC line A mouse ESC line in which the targeted gene, forkhead box A2 (NCBI Gene ID: 15376), has been modified (allele type: Cassette Acceptor) to Forkhead box A2 loxed cassette acceptor (MGI ID: Foxa2{LCA}). This ES cell line contains a loxed cassette acceptor (LCA) allele that may be utilized for the exchange of DNAs of interest into the Foxa2 locus by recombinase mediated cassette exchange (RMCE). This LCA uses a Lox66/71 and Lox2272 strategy for RMCE and allows for manipulation of a 4.7 kb region of the gene (contains a promoter and all exons: exon 1, 2 and 3). This leads to a variety of experiments that can be performed using a wide range of cassette designs. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ngn3 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Ngn3 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Ngn3" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Nkx6.1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Nkx6.1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Nkx6.1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse PDX1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "PDX1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Prox1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Prox1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Polyclonal mouse Prox1 raised in chicken A polyclonal antibody raised in chicken that targets the mouse "Prox1" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Nkx2.2{T2A.cCreGgu} mouse strain A mouse strain in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{T2A.cCre} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this line, one of the two bi-partite Cre molecules, cCre, is placed downstream of Nkx2.2 coding sequence fused with 2a peptide using RMCE in the Nkx2.2[LCA] allele. The presence of the T2A sequence allows for transcription of Nkx2.2 and cCre from the native Nkx2.2 locus at the same time. This mouse line allows the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Nkx2.2{T2A.cCreGgu} Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Rosa26{mIre1.N906A.CherryMgn} mouse strain A mouse strain in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Rosa26.mIre1.N906A.Cherry using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This mouse contains bidirectional TetO-regulated genes inserted into the Rosa26.LCA allele by RMCE. In one direction the TetO/CMV promoter drives the expression of point mutant (N906A) of IRE1, an ER transmembrane kinase response to unfolded protein response (UPR). In the other direction it drives the expression of red fluorescent protein mCherry. This mouse can be used to simultaneously over-express Ire1 (N906A) and mCherry upon administration of doxycyline when the effector protein rtTA is expressed. This line can be used to study the roles of IRE1 in the response to ER stress, diet induced obesity and insulin resistance. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Rosa26{mIre1.N906A.CherryMgn} Vanderbilt University Medical Center Sst{rTTA.LCA.Mgn} mouse strain A mouse strain in which the targeted gene, Somatostatin (NCBI Gene ID: 20604), has been modified (allele type: Gene Replacement) to (MGI ID: Sst{rTTA.LCA}). TheSst.rTTA.LCA mice are designed to express a tet-inducible reverse-transactivator under control of somatostatin promoter. Lox66 and Lox2272 sites are inserted flanking 500bp of Sst promoter region, Sst exons I and II and Sst poly A site. The rTTA-beta-globin poly A cassete is put in place of Sst coding exons (I and II). The mice can be used to drive tet-inducible expression in somatostatin expressing cells (Delta-cells). Additionally, the Lox66 and Lox2272 sites allow for manipulations of the flanked region by RMCE in the future. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Sst{rTTA.LCA.Mgn} Vanderbilt University Medical Center Monoclonal human ghrelin raised in mouse A monoclonal antibody raised in mouse that targets the human "ghrelin" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Gallbladder mucosal epithelium raised in mouse A monoclonal antibody raised in mouse that targets the human "Gallbladder mucosal epithelium" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Gallbladder mucosal epithelium raised in mouse A monoclonal antibody raised in mouse that targets the human "Gallbladder mucosal epithelium" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Gallbladder mucosal epithelium raised in mouse A monoclonal antibody raised in mouse that targets the human "Gallbladder mucosal epithelium" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Gallbladder mucosal epithelium raised in mouse A monoclonal antibody raised in mouse that targets the human "Gallbladder mucosal epithelium" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Alpha cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Alpha cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center Monoclonal human Pancreatic duct cells raised in mouse A monoclonal antibody raised in mouse that targets the human "Pancreatic duct cells" antigen. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(HLA-A/H2-D/B2M)1Dvs/SzJ mouse strain A mouse strain that is immunodeficient and express human HLA class 1 heavy and light chains. This strain may be useful as a human hematopoietic engraftment host that supports the maturation of human T cells with transplantation http://jaxmice.jax.org/strain/014570.html. NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} Tg(HLA-A/H2-D/B2M)1Dvs/SzJ Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} H2-Ab1{tm1Gru} Tg(HLA-DRB1)31Dmz/SzJ mouse strain A mouse strain that lacks expression of the murine Prkdc gene, the X-linked Il2rg gene, and MHC class II, but express the human leukocyte antigen DR4 gene. These mice may be useful for targeting human CD4{+} T cells in transplantation studies in the absence of xeno-GVHD.More details are available at http://jaxmice.jax.org/strain/017637.html. NOD.Cg-Prkdc{scid} Il2rg{tm1Wjl} H2-Ab1{tm1Gru} Tg(HLA-DRB1)31Dmz/SzJ Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center embryonic day 15.5 15.5 days after fertilization Person: Chris Stoeckert, Jie Zheng embryonic day 16.5 16.5 days after fertilization Person: Chris Stoeckert, Jie Zheng embryonic day 8.5 Person: Chris Stoeckert, Jie Zheng 8.5 days after fertilization embryonic day 17.5 Person: Chris Stoeckert, Jie Zheng 17.5 days after fertilization embryonic day 8.25 8.25 days after fertilization Person: Chris Stoeckert, Jie Zheng embryonic day 18.5 Person: Chris Stoeckert, Jie Zheng 18.5 days after fertilization embryonic day 8 8 days after fertilization Person: Chris Stoeckert, Jie Zheng embryonic day 18 18 days after fertilization Person: Chris Stoeckert, Jie Zheng embryonic day 11.5 Person: Chris Stoeckert, Jie Zheng 11.5 days after fertilization postnatal day 0 Person: Jie Zheng 0 day after birth embryonic day 12.5 12.5 days after fertilization Person: Chris Stoeckert, Jie Zheng postnatal day 4 4 days after birth Person: Jie Zheng embryonic day 9.5 Person: Chris Stoeckert, Jie Zheng 9.5 days after fertilization postnatal day 12 12 days after birth Person: Jie Zheng embryonic day 10.5 Person: Chris Stoeckert, Jie Zheng 10.5 days after fertilization postnatal day 60 Person: Jie Zheng 60 days after birth embryonic day 13.5 Person: Chris Stoeckert, Jie Zheng 13.5 days after fertilization embryonic day 14.5 Person: Chris Stoeckert, Jie Zheng 14.5 days after fertilization postnatal day 1 Person: Jie Zheng 1 day after birth postnatal day 8 Person: Jie Zheng 8 days after birth embryonic day 11.25 11.25 days after fertilization Person: Chris Stoeckert, Jie Zheng age 4 weeks Person: Jie Zheng 4 weeks after birth age 8 weeks Person: Jie Zheng age 8 weeks age 10 weeks age 10 weeks Person: Jie Zheng adult Person: Jie Zheng age of an organism that fully grown or developed.