# splitcode config file for demultiplexing barcodes into wells # A hamming distance error of 1 is permitted # For R1: ## If there is a hamming distance collision (e.g. CATTCCTA vs. CATTCCAT), the read is considered unassigned ### (Putting $ after the barcode filenames AND giving each filename a different ID ensures that every single barcode gets a unique ID) ### (Each barcode having a unique ID allows the "hamming distance collision = unassigned" to happen since multiple IDs being mapped is not allowed) ### (Note: Barcodes are complex enough so that each barcode is at least two hamming distances away from other barcodes [i.e. cases like CATTCCTA vs. CATTCCTT won't happen]; but there are some collisions). tags distances ids groups locations A1_A8.txt$ 1 exp1 A1_A8 1,78,86 B1_B8.txt$ 1 exp2 B1_B8 1,78,86 C1_C8.txt$ 1 exp3 C1_C8 1,78,86 D1_D8.txt$ 1 exp4 D1_D8 1,78,86