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            "annotation": "This workflow is deliberately a simple/crude assessment, and there is no need to run BLASTX on all the sequences - a sample of 1000 should be enough.", 
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            "inputs": [], 
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                "top": 119
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            "annotation": "We only want one line per query, so limit this to the best scoring target sequence. Assumes current NCBI nr database is available locally as \"nr\".", 
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            "annotation": "Having a tabular file of all 1000 sequences is used in the \"join\" step to count the sequences giving no BLAST hit.", 
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                    "output_name": "output_file"
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            }, 
            "inputs": [], 
            "label": null, 
            "name": "FASTA-to-Tabular", 
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                    "type": "tabular"
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            ], 
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        "4": {
            "annotation": "Some BLAST matches will give multiple HSPs, and thus multiple lines in the tabular output. We only want one line per query.", 
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                "input": {
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                    "output_name": "output1"
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            "inputs": [], 
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            "name": "Unique", 
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                    "name": "outfile", 
                    "type": "input"
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                "top": 376
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                    "action_type": "HideDatasetAction", 
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                        "newname": "One HSP per BLAST hit"
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                    "action_type": "RenameDatasetAction", 
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        "5": {
            "annotation": "We don't need all the columns in this join, but the key is to assign \"None\" to the sequences with no BLAST hits.", 
            "id": 5, 
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                "input2": {
                    "id": 4, 
                    "output_name": "outfile"
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            "inputs": [], 
            "label": null, 
            "name": "Join two Datasets", 
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                    "type": "input"
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                    "action_type": "HideDatasetAction", 
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            "annotation": "Here we make a tally table of the BLAST species name column", 
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                    "output_name": "out_file1"
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                    "type": "tabular"
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            "position": {
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        "7": {
            "annotation": "Sorting the counts makes the results easier to interpret directly.", 
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                        "newname": "Top BLAST hit species counts"
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