library(gplots) library(RColorBrewer) ######################################################### ### reading in data and transform it to matrix format ######################################################### data <- read.csv("dataset.csv", comment.char="#") rnames <- data[,1] # assign labels in column 1 to "rnames" mat_data <- data.matrix(data[,2:ncol(data)]) # transform column 2-5 into a matrix rownames(mat_data) <- rnames # assign row names ######################################################### ### customizing and plotting heatmap ######################################################### # creates a own color palette from red to green my_palette <- colorRampPalette(c("red", "yellow", "green"))(n = 299) # (optional) defines the color breaks manually for a "skewed" color transition col_breaks = c(seq(-1,0,length=100), # for red seq(0.01,0.7,length=100), # for yellow seq(0.71,1,length=100)) # for green # creates a 5 x 5 inch image png("h1_simple.png", width = 5*300, # 5 x 300 pixels height = 5*300, res = 300, # 300 pixels per inch pointsize = 8) # smaller font size heatmap.2(mat_data, cellnote = mat_data, # same data set for cell labels main = "Correlation", # heat map title notecol="black", # change font color of cell labels to black density.info="none", # turns off density plot inside color legend trace="none", # turns off trace lines inside the heat map margins =c(12,9), # widens margins around plot col=my_palette, # use on color palette defined earlier breaks=col_breaks, # enable color transition at specified limits dendrogram="row", # only draw a row dendrogram Colv="NA") # turn off column clustering ############################################################################## # NOTE ############################################################################## # The color breaks above will yield a warning # "...unsorted 'breaks' will be sorted before use" since they contain # (due to the negative numbers). To avoid this warning, you can change the # manual breaks to: # # col_breaks = c(seq(0,1,length=100), # for red # seq(1.01,1.7,length=100), # for yellow # seq(1.71,2,length=100)) # for green # # However, the problem is then that our heatmap contains negative values # which will then not be colored correctly. Remember that you don't need to # provide manual color breaks at all, this is entirely optional. ############################################################################## dev.off()