FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | PiuraChilensis_Coquimbo.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 758154 |
| Filtered Sequences | 0 |
| Sequence length | 55-1201 |
| %GC | 43 |
![[FAIL]](Icons/error.png)
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per base GC content
Per sequence GC content
![[WARN]](Icons/warning.png)
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GACTCGCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAAAG | 2750 | 0.36272314068118083 | No Hit |
| GACTGCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGA | 2136 | 0.2817369558163645 | No Hit |
| GACTCCGCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAAA | 1642 | 0.216578689817636 | No Hit |
| GACTCACCGCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAA | 1211 | 0.15973008122360366 | No Hit |
| GACTCAAAGTTGTACGTTGTTAAGTGCTATCGATAATGCTTGGATGAGTA | 1094 | 0.14429786032916794 | No Hit |
| GACTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAA | 1079 | 0.14231937047090695 | No Hit |
| GACTGTCAAAGTTGTACGTTGTTAAGTGCTATCGATAATGCTTGGATGAG | 911 | 0.12016028405838393 | No Hit |
| GACTCAAAATGTGGCTTGTTTGTTTAATAAAAACTTTGTCTATTGTGGCT | 904 | 0.11923698879119547 | No Hit |
| GACTCCCGTCTGTCCCTCTTAATCATTACCTCGCGCTCCGAAAACCAACA | 885 | 0.11673090163739822 | No Hit |
| GACTGTTCTAAACCCAACTCACGTATATTTTTGATTGGTGAACAAACAAA | 865 | 0.1140929151597169 | No Hit |
| GACTACCGCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAA | 860 | 0.11343341854029657 | No Hit |
| GACTCTTGAAAAAATTCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAA | 848 | 0.11185062665368777 | No Hit |
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TTTTT | 1924105 | 2.8012276 | 11.527032 | 1000-1099 |
| AAAAA | 1953400 | 2.608366 | 31.012491 | 1000-1099 |
| GGAGG | 584015 | 2.2087417 | 5.855697 | 500-599 |
| TTTTC | 1131030 | 2.1693327 | 5.237959 | 6 |
| GAAAA | 1148120 | 1.9888434 | 6.567923 | 5 |
| GGGAG | 513765 | 1.9430567 | 6.0033593 | 500-599 |
| GACTG | 633690 | 1.9421166 | 531.1185 | 1 |
| AGGCA | 626470 | 1.8870802 | 6.890259 | 500-599 |
| TCTTC | 745095 | 1.8827648 | 9.999619 | 4 |
| ATTTT | 1315375 | 1.8821787 | 7.121207 | 5 |
| GTCGT | 581885 | 1.8144462 | 5.316891 | 500-599 |
| AAAAT | 1330660 | 1.8078105 | 5.595341 | 7 |
| CGTGG | 439300 | 1.7466086 | 5.856552 | 500-599 |
| GTGGG | 447780 | 1.7230351 | 5.6570425 | 500-599 |
| GGGGA | 428670 | 1.6212277 | 5.185089 | 600-699 |
| TGAAA | 908880 | 1.6018738 | 12.989435 | 4 |
| TGGGA | 527645 | 1.5650756 | 12.191784 | 4 |
| TCTTT | 803975 | 1.5420364 | 11.678703 | 4 |
| GGCAC | 381855 | 1.5418046 | 5.0076427 | 500-599 |
| TTCCT | 607820 | 1.5358877 | 5.0160327 | 15-19 |
| GACTC | 475290 | 1.5050887 | 354.62384 | 1 |
| CTGCC | 351735 | 1.4930001 | 36.45636 | 3 |
| TGCTG | 477055 | 1.487563 | 6.703566 | 4 |
| CTTTT | 773505 | 1.4835944 | 15.314418 | 3 |
| CTTTC | 585600 | 1.4797403 | 9.055892 | 3 |
| TTTTG | 795950 | 1.4775183 | 6.082245 | 1100-1196 |
| CTTTG | 601515 | 1.471045 | 5.6696434 | 3 |
| CTGCT | 455195 | 1.4665922 | 31.273066 | 3 |
| ACTTT | 777570 | 1.4658283 | 29.741703 | 2 |
| AGGGG | 384965 | 1.4559357 | 6.158252 | 900-999 |
| CTTGG | 466060 | 1.4532782 | 5.1913543 | 3 |
| GTACG | 470420 | 1.4417311 | 11.47786 | 700-799 |
| GTTTT | 771370 | 1.4318907 | 11.28905 | 5 |
| GTGAA | 624020 | 1.426777 | 7.2103868 | 5 |
| AAAAC | 796640 | 1.4258714 | 13.646637 | 1000-1099 |
| TCTTG | 580015 | 1.4184653 | 13.685964 | 4 |
| CGTAC | 444545 | 1.4077293 | 10.359745 | 700-799 |
| CTGCA | 443860 | 1.4055601 | 24.249657 | 3 |
| CTGGG | 351015 | 1.3955971 | 35.70472 | 3 |
| TCAAA | 765740 | 1.3944664 | 8.934954 | 4 |
| CTGGA | 453550 | 1.3900282 | 43.46554 | 3 |
| TGGAA | 607575 | 1.3891767 | 15.161173 | 4 |
| TCTGG | 444810 | 1.3870159 | 9.679335 | 4 |
| TGAAG | 604115 | 1.3812656 | 5.2533436 | 4 |
| TCTCC | 411840 | 1.3710248 | 6.5351744 | 4 |
| TGCCC | 322805 | 1.3702017 | 7.253943 | 4 |
| TGCAG | 446055 | 1.3670577 | 6.278704 | 4 |
| CTCCA | 417615 | 1.3664207 | 14.763739 | 3 |
| GGAAA | 604430 | 1.3582983 | 6.4340672 | 5 |
| TATTT | 944475 | 1.3514555 | 13.967072 | 4 |
| ACTTC | 543225 | 1.3491361 | 7.4579167 | 2 |
| CTCTC | 403215 | 1.3423121 | 34.583305 | 3 |
| CTCTG | 414540 | 1.335606 | 30.053223 | 3 |
| ACTGG | 435330 | 1.3341882 | 120.26183 | 2 |
| TTTTA | 931610 | 1.3330466 | 7.7208138 | 1000-1099 |
| GTTTG | 556355 | 1.3168205 | 6.856384 | 5 |
| GTTGG | 435425 | 1.3140606 | 5.3533483 | 5 |
| ACTGC | 413845 | 1.3105125 | 96.16796 | 2 |
| TGTTT | 705860 | 1.3102849 | 25.503721 | 4 |
| TGTTG | 551250 | 1.3047376 | 20.623308 | 4 |
| TCGAC | 410875 | 1.3011074 | 6.561372 | 4 |
| TGGTT | 542120 | 1.2831279 | 6.070372 | 4 |
| TCTGC | 396915 | 1.2788198 | 6.458562 | 4 |
| CCCCG | 228670 | 1.2787504 | 32.136642 | 1100-1196 |
| TAAAA | 937765 | 1.2740307 | 9.402254 | 1000-1099 |
| CTCCC | 289500 | 1.2696902 | 28.501585 | 3 |
| GTTGT | 532470 | 1.2602878 | 8.765368 | 5 |
| ACGTA | 526975 | 1.2449518 | 11.07957 | 700-799 |
| TGGAG | 418450 | 1.2411865 | 7.5535216 | 4 |
| CTGTG | 397695 | 1.240101 | 42.75994 | 3 |
| TGCAA | 523985 | 1.2378882 | 7.8785815 | 4 |
| AAACC | 513785 | 1.232652 | 22.444633 | 1100-1196 |
| CCCGG | 226715 | 1.2270207 | 42.90304 | 1100-1196 |
| TGTGA | 526585 | 1.2249957 | 15.5462265 | 4 |
| GGGAA | 419990 | 1.224402 | 5.274472 | 900-999 |
| TGTGG | 402350 | 1.2142441 | 10.573087 | 4 |
| CCGGG | 231105 | 1.2105314 | 37.55953 | 1100-1196 |
| GTTTC | 494650 | 1.2096995 | 6.1203213 | 5 |
| GGGGG | 245850 | 1.2062215 | 45.406796 | 1100-1196 |
| CTTGA | 500455 | 1.202918 | 9.019414 | 3 |
| ACTGT | 497215 | 1.1951303 | 184.52469 | 2 |
| GCTTG | 382260 | 1.1919712 | 7.4238863 | 6 |
| CCTTG | 369375 | 1.1900887 | 6.463293 | 1000-1099 |
| CTGTT | 486620 | 1.1900616 | 85.729576 | 3 |
| TACGT | 493700 | 1.1866815 | 9.159507 | 700-799 |
| CGGCC | 219250 | 1.186619 | 7.6272078 | 1100-1196 |
| TGTCG | 379950 | 1.1847682 | 15.250816 | 4 |
| TTTGG | 496390 | 1.174891 | 5.8163786 | 1100-1196 |
| AACCC | 363220 | 1.1680721 | 33.853794 | 1100-1196 |
| GGTTT | 490770 | 1.1615891 | 17.286097 | 1100-1196 |
| CTCCG | 273340 | 1.160239 | 29.862955 | 3 |
| TGTTC | 473295 | 1.1574744 | 15.569529 | 4 |
| CGGGG | 227880 | 1.1552287 | 31.38963 | 1100-1196 |
| CTCTT | 456955 | 1.1546701 | 46.2034 | 3 |
| TCGCC | 271895 | 1.1541054 | 6.010095 | 4 |
| CTGTC | 356775 | 1.1494929 | 56.0041 | 3 |
| TCGAA | 484940 | 1.1456465 | 7.933719 | 4 |
| CTGGT | 366695 | 1.1434362 | 22.164335 | 3 |
| CTTGC | 354790 | 1.1430974 | 5.631324 | 3 |
| CTCCT | 341845 | 1.1380099 | 11.79251 | 3 |
| ACTTG | 473220 | 1.1374549 | 20.4508 | 2 |
| TGCTC | 351515 | 1.1325456 | 5.0381794 | 4 |
| CTCGA | 356345 | 1.1284286 | 29.226051 | 3 |
| GGACG | 287765 | 1.1245115 | 5.422355 | 600-699 |
| CCCTT | 334205 | 1.1125761 | 5.4668717 | 1000-1099 |
| TGGAT | 476270 | 1.1079478 | 7.0461745 | 4 |
| GTTCT | 452920 | 1.107646 | 5.352903 | 5 |
| CGGAA | 366365 | 1.1035804 | 5.2674513 | 700-799 |
| TCCGG | 268335 | 1.1023427 | 5.880616 | 4 |
| TGCTT | 449695 | 1.0997591 | 10.35009 | 4 |
| CTGAA | 463200 | 1.0942868 | 32.157177 | 3 |
| GACTA | 462270 | 1.0920897 | 182.98051 | 1 |
| CCGGA | 269175 | 1.0868398 | 10.942369 | 1100-1196 |
| GGAAC | 358765 | 1.0806873 | 5.599867 | 1100-1196 |
| GTCAA | 456915 | 1.0794388 | 6.2370286 | 5 |
| CTGGC | 262250 | 1.077345 | 36.838593 | 3 |
| TGTCC | 333145 | 1.0733595 | 8.697753 | 4 |
| TGTCA | 446380 | 1.072941 | 16.150389 | 4 |
| AACGT | 452230 | 1.0683706 | 11.200063 | 700-799 |
| CCGAA | 342655 | 1.0664783 | 6.174324 | 1100-1196 |
| TCGAG | 347735 | 1.0657291 | 5.0785975 | 4 |
| TGGGG | 276960 | 1.0657283 | 9.858954 | 4 |
| GACTT | 442380 | 1.0633262 | 73.18712 | 1 |
| ACCGG | 263335 | 1.0632598 | 20.262005 | 1100-1196 |
| TCGAT | 442155 | 1.0627854 | 5.148676 | 4 |
| GGGTT | 352140 | 1.0627162 | 18.0537 | 1000-1099 |
| ACTAC | 434305 | 1.060138 | 15.40078 | 2 |
| TGGCA | 344860 | 1.0569179 | 11.055287 | 4 |
| GAACG | 350610 | 1.0561227 | 6.035622 | 700-799 |
| AAGGG | 360395 | 1.0506638 | 6.270761 | 900-999 |
| TGCCA | 331675 | 1.0503068 | 10.232127 | 4 |
| AACGG | 347605 | 1.0470706 | 19.433308 | 1000-1099 |
| ACGGA | 345010 | 1.0392538 | 6.3941007 | 700-799 |
| CCCGT | 244685 | 1.0386078 | 14.091068 | 1100-1196 |
| GTAAC | 439000 | 1.0371155 | 8.945132 | 700-799 |
| TCCGA | 327315 | 1.0365001 | 6.8159432 | 4 |
| TGGCT | 331985 | 1.0352027 | 5.8625884 | 4 |
| TCGGA | 335655 | 1.0287067 | 6.9939995 | 4 |
| TTTAC | 543305 | 1.0242059 | 5.9703727 | 1000-1099 |
| CCGGT | 249225 | 1.0238372 | 16.715155 | 1100-1196 |
| ACGTT | 425615 | 1.023029 | 10.1293125 | 800-899 |
| CCCCC | 177005 | 1.0227443 | 37.616005 | 1100-1196 |
| AACCG | 327310 | 1.0187185 | 18.598038 | 1100-1196 |
| TGGCC | 246655 | 1.0132794 | 5.422525 | 4 |
| TCCCA | 309180 | 1.0116255 | 6.40619 | 4 |
| GCCGA | 249790 | 1.0085696 | 39.688885 | 1100-1196 |
| CCGTT | 312930 | 1.0082287 | 13.4163475 | 1000-1099 |
| TGGAC | 328005 | 1.0052613 | 6.056168 | 4 |
| CTGAC | 317255 | 1.0046433 | 12.744824 | 3 |
| TTTAA | 712020 | 1.0013708 | 14.990125 | 1100-1196 |
| ACTGA | 422555 | 0.99826497 | 63.608986 | 2 |
| CTCGT | 309705 | 0.9978382 | 13.727397 | 3 |
| AAACG | 429190 | 0.9965606 | 12.380208 | 1100-1196 |
| TCCCG | 233210 | 0.98990023 | 9.55451 | 4 |
| TTAAA | 715725 | 0.9893284 | 14.187515 | 1100-1196 |
| TGGCG | 248605 | 0.9884262 | 8.58863 | 4 |
| GTAAA | 560810 | 0.9884108 | 7.339123 | 1000-1099 |
| ACGGG | 252155 | 0.98535675 | 16.73065 | 1000-1099 |
| ACTCC | 300425 | 0.9829795 | 70.635925 | 2 |
| CTCGC | 231120 | 0.98102885 | 32.658825 | 3 |
| GGTAC | 319830 | 0.9802068 | 6.650653 | 1000-1099 |
| GTTAC | 407760 | 0.9801119 | 7.5969844 | 800-899 |
| TGTCT | 398430 | 0.9743871 | 7.5152307 | 4 |
| TCGGG | 244790 | 0.9732582 | 5.81511 | 4 |
| TTACC | 391370 | 0.9719937 | 7.7989326 | 800-899 |
| ACTCT | 389880 | 0.96829337 | 103.97933 | 2 |
| TCTCG | 300205 | 0.96723014 | 11.012141 | 4 |
| TCTGA | 402275 | 0.9669279 | 6.9313893 | 4 |
| GTACC | 304575 | 0.96448994 | 5.5144305 | 700-799 |
| GGTAA | 420880 | 0.962312 | 11.934706 | 1000-1099 |
| CGTTT | 391305 | 0.9569624 | 9.976052 | 1100-1196 |
| CTATT | 505285 | 0.952533 | 37.94024 | 3 |
| TCTCA | 381780 | 0.9481765 | 7.5418406 | 4 |
| CGTAA | 398655 | 0.94180244 | 8.703203 | 800-899 |
| CTCGG | 226650 | 0.93109727 | 22.840353 | 3 |
| TTAAC | 501795 | 0.9297399 | 13.039793 | 1100-1196 |
| CTCAC | 282625 | 0.9247385 | 14.381918 | 3 |
| GGGGT | 239575 | 0.92187256 | 25.19886 | 1100-1196 |
| GGGTA | 310635 | 0.92139083 | 11.816108 | 1100-1196 |
| ACCCG | 220255 | 0.9188858 | 23.821215 | 1100-1196 |
| TACCC | 280420 | 0.91752386 | 6.140764 | 1000-1099 |
| TATTC | 485455 | 0.91515064 | 5.763707 | 4 |
| GTTAA | 509975 | 0.9144903 | 12.462035 | 1100-1196 |
| CGGTT | 292500 | 0.91207975 | 16.891024 | 1100-1196 |
| ACTAG | 385975 | 0.9118466 | 30.723207 | 2 |
| ACGGT | 296590 | 0.9089813 | 12.941811 | 1000-1099 |
| CTCAA | 371855 | 0.90769756 | 23.205915 | 3 |
| GGCCC | 167265 | 0.9052672 | 6.6917953 | 1100-1196 |
| ACCCC | 209725 | 0.90404683 | 27.271254 | 1100-1196 |
| ACCGT | 284655 | 0.9014097 | 11.39483 | 900-999 |
| TTACG | 374235 | 0.8995295 | 7.8093734 | 800-899 |
| ACTCG | 283725 | 0.89846474 | 84.68791 | 2 |
| TAAAC | 488145 | 0.88894635 | 12.65568 | 1100-1196 |
| CTGAT | 368020 | 0.88459086 | 14.093224 | 3 |
| GCCCG | 162400 | 0.8789369 | 5.9607587 | 1100-1196 |
| TATTA | 622390 | 0.87531704 | 6.513715 | 4 |
| CTAGT | 362900 | 0.8722843 | 5.198474 | 3 |
| TGCCT | 270660 | 0.87203914 | 5.4058084 | 4 |
| CGGGT | 217985 | 0.86668444 | 16.32287 | 1000-1099 |
| TAACC | 353915 | 0.8639061 | 15.802609 | 1100-1196 |
| CGGTA | 280555 | 0.85983765 | 11.863327 | 1000-1099 |
| TACCG | 269280 | 0.8527221 | 8.2006 | 800-899 |
| ACTCA | 348575 | 0.8508711 | 53.177937 | 2 |
| TGTGC | 270210 | 0.8425746 | 5.5714808 | 4 |
| CTACC | 256160 | 0.83814603 | 5.574584 | 3 |
| CTAGG | 273475 | 0.8381392 | 5.595126 | 3 |
| TCGCT | 260130 | 0.83811253 | 10.686289 | 4 |
| CTCAG | 264370 | 0.83717376 | 14.378987 | 3 |
| TCCGC | 196850 | 0.83556396 | 9.015143 | 4 |
| TACGG | 271730 | 0.83279115 | 9.346864 | 1000-1099 |
| CTCAT | 333620 | 0.82856786 | 8.301711 | 3 |
| TATCA | 443630 | 0.8219702 | 6.159815 | 4 |
| GTTTA | 449465 | 0.82003903 | 12.534504 | 1100-1196 |
| TAACG | 344960 | 0.8149507 | 11.527114 | 700-799 |
| CTGTA | 334210 | 0.8033235 | 25.524878 | 3 |
| TAGAA | 452190 | 0.7969713 | 6.3895955 | 4 |
| GGTTA | 342195 | 0.79604894 | 10.95975 | 1100-1196 |
| CTGCG | 191685 | 0.78745806 | 18.142315 | 3 |
| CCGTA | 248395 | 0.78658605 | 7.7701216 | 800-899 |
| ACTAT | 423940 | 0.785488 | 65.83429 | 2 |
| CTGAG | 253475 | 0.77684355 | 10.498808 | 3 |
| TCGCA | 245010 | 0.7758669 | 5.904413 | 4 |
| TGCCG | 188835 | 0.77575004 | 8.085847 | 4 |
| TGCGA | 251290 | 0.770147 | 5.7621017 | 4 |
| ACTAA | 421790 | 0.76810926 | 41.16487 | 2 |
| CTAAA | 421095 | 0.7668436 | 21.439827 | 3 |
| ACGGC | 189680 | 0.76586527 | 6.6148148 | 1100-1196 |
| CGTTA | 315390 | 0.7580868 | 11.171172 | 1100-1196 |
| TGTAA | 409335 | 0.734022 | 8.244863 | 4 |
| TGTTA | 396170 | 0.7228035 | 11.378845 | 4 |
| CCGCT | 164075 | 0.6964447 | 6.2746058 | 5 |
| ACTTA | 369340 | 0.6843236 | 5.856955 | 2 |
| CTATC | 268330 | 0.66641575 | 18.149124 | 3 |
| CTAAC | 271010 | 0.6615351 | 6.538929 | 3 |
| CGCTT | 204000 | 0.6572673 | 7.7038913 | 5 |
| CTATG | 246145 | 0.5916462 | 9.611567 | 3 |
| CTCTA | 228935 | 0.56857556 | 9.602677 | 3 |
| CTAGA | 237960 | 0.56216854 | 14.519434 | 3 |
| CTAAT | 293945 | 0.5446296 | 9.234796 | 3 |
| CTAAG | 198575 | 0.46912348 | 7.4863915 | 3 |
| CTAGC | 122630 | 0.38832933 | 9.090543 | 3 |
| CTATA | 202805 | 0.37576282 | 7.596364 | 3 |