FastQCFastQC Report
Sun 7 Dec 2014
PiuraChilensis_Coquimbo_trimmer_out.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename PiuraChilensis_Coquimbo_trimmer_out.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 758154
Filtered Sequences 0
Sequence length 36-431
%GC 43

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGTC 2549 0.33621137658048367 No Hit
TTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGTCG 1935 0.25522519171566727 No Hit
CCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGT 1554 0.2049715493158382 No Hit
TCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGG 1540 0.20312495878146128 No Hit
TTCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAG 1476 0.19468340205288107 No Hit
TAATCATTACCTCGCGCTCCGAAAACCAACAAAATAGAACCGAGGTCCTA 987 0.13018463267357291 No Hit
ACTCACGTATATTTTTGATTGGTGAACAAACAAACTTCTCTATCTTCTAC 979 0.12912943808250038 No Hit
CACGTATATTTTTGATTGGTGAACAAACAAACTTCTCTATCTTCTACGAC 944 0.12451296174655808 No Hit
TTAAGTGCTATCGATAATGCTTGGATGAGTATCAAAAAATTAGGGAATTT 921 0.12147927729722457 No Hit
TTGTTTAATAAAAACTTTGTCTATTGTGGCTTATAATAGGTCTAGTCTGC 903 0.1191050894673114 No Hit
GTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGTCGCCCC 861 0.11356531786418063 No Hit
AACTCACGTATATTTTTGATTGGTGAACAAACAAACTTCTCTATCTTCTA 858 0.11316961989252844 No Hit
CTCACGTATATTTTTGATTGGTGAACAAACAAACTTCTCTATCTTCTACG 826 0.10894884152823833 No Hit
TGTTAAGTGCTATCGATAATGCTTGGATGAGTATCAAAAAATTAGGGAAT 783 0.1032771706012235 No Hit
AAAATCTAAACTAGGTCGCCCCGTTCTAAACCCAACTCACGTATATTTTT 771 0.1016943787146147 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1511905 2.669551 8.158263 20-24
CTTTC 470270 1.4407078 5.9173465 1
GGTCG 266635 1.3453084 6.1114 45-49
AGGTC 316990 1.224315 5.570803 45-49
CCCCG 175820 1.2098264 5.1289434 50-59
TTTCG 384780 1.158497 5.965849 2
CGTAC 291225 1.1445179 6.58576 5
TTCGT 367510 1.1065003 5.4523787 3
GTCGC 193515 0.9934948 5.3548956 45-49
TCGTA 296025 0.88321173 5.049443 4
GTACT 288820 0.8617151 5.2666264 6
Produced by FastQC (version 0.10.1)