FastQCFastQC Report
Sun 7 Dec 2014
PiuraChilensis_Valdivia_trimmer_out.fastq

Summary

[OK] Basic Statistics

Measure Value
Filename PiuraChilensis_Valdivia_trimmer_out.fastq
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 683347
Filtered Sequences 0
Sequence length 32-431
%GC 44

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[OK] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
CTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGTC 1084 0.15863097372198898 No Hit
TTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGTCG 909 0.13302172980930624 No Hit
CCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGGT 793 0.1160464595586137 No Hit
TCCTTTCGTACTATTTCTCTGTTTTTTTAAAAGAAAAAATCTAAACTAGG 747 0.10931488687299426 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 1141385 2.462982 6.093003 20-24
Produced by FastQC (version 0.10.1)