{ "metadata": { "name": "", "signature": "sha256:e992bbd994a6cfb3adc4bb6828290d83d64e46ed9514a3a547212cff36f559fb" }, "nbformat": 3, "nbformat_minor": 0, "worksheets": [ { "cells": [ { "cell_type": "heading", "level": 1, "metadata": {}, "source": [ "Mitochondria Methylation - Sperm" ] }, { "cell_type": "code", "collapsed": true, "input": [ "! /Volumes/Bay3/Software/BSMAP/bsmap-2.74/bsmap -a /Volumes/web/trilobite/Crassostrea_gigas_HTSdata/filtered_174gm_A_NoIndex_L006_R1.fastq -b /Volumes/web/trilobite/Crassostrea_gigas_HTSdata/filtered_174gm_A_NoIndex_L006_R2.fastq -d /Volumes/web/cnidarian/Cgigas_mito_genome.fasta -o /Volumes/web/cnidarian/BiGo_mito.sam -p 3" ], "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": [ "\r\n", "BSMAP v2.74\r\n", "Start at: Mon Dec 2 12:26:22 2013\r\n", "\r\n", "Input reference file: /Volumes/web/cnidarian/Cgigas_mito_genome.fasta \t(format: FASTA)\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Load in 1 db seqs, total size 18224 bp. 0 secs passed\r\n", "total_kmers: 43046721\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Create seed table. 3 secs passed\r\n", "max number of mismatches: read_length * 8% \tmax gap size: 0\r\n", "kmer cut-off ratio: 5e-07\r\n", "max multi-hits: 100\tmax Ns: 5\tseed size: 16\tindex interval: 4\r\n", "quality cutoff: 0\tbase quality char: '!'\r\n", "min fragment size:28\tmax fragemt size:500\r\n", "start from read #1\tend at read #4294967295\r\n", "additional alignment: T in reads => C in reference\r\n", "mapping strand (read_1): ++,-+\r\n", "mapping strand (read_2): +-,--\r\n", "Pair-end alignment(3 threads)\r\n", "Input read file #1: /Volumes/web/trilobite/Crassostrea_gigas_HTSdata/filtered_174gm_A_NoIndex_L006_R1.fastq" ] }, { "output_type": "stream", "stream": "stdout", "text": [ " \t(format: FASTQ)\r\n", "Input read file #2: 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"Thread #2: \t171300000 read pairs finished. 2967 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #1: \t171350000 read pairs finished. 2968 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #0: \t171400000 read pairs finished. 2969 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #2: \t171450000 read pairs finished. 2970 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #1: \t171500000 read pairs finished. 2971 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #0: \t171550000 read pairs finished. 2972 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Thread #2: \t171582441 read pairs finished. 2972 secs passed\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "Total number of aligned reads: \r\n", "pairs: 11907 (0.0069%)\r\n", "single a: 405 (0.00024%)\r\n", "single b: 357 (0.00021%)\r\n", "Done.\r\n", "Finished at Mon Dec 2 13:15:54 2013\r\n", "Total time consumed: 2972 secs\r\n" ] } ], "prompt_number": 1 }, { "cell_type": "code", "collapsed": false, "input": [ "!python /Volumes/Bay3/Software/BSMAP/bsmap-2.74/methratio.py -d /Volumes/web/cnidarian/Cgigas_mito_genome.fasta -u -z -g -o /Volumes/web/cnidarian/BiGo_mito_methratio_A.txt -s /Volumes/Bay3/Software/BSMAP/bsmap-2.74/samtools /Volumes/web/cnidarian/BiGo_mito.sam" ], "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": [ "@ Mon Dec 2 13:26:03 2013: reading reference /Volumes/web/cnidarian/Cgigas_mito_genome.fasta ...\r\n", "@ Mon Dec 2 13:26:03 2013: reading /Volumes/web/cnidarian/BiGo_mito.sam ...\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "[samopen] SAM header is present: 1 sequences.\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "@ Mon Dec 2 13:26:05 2013: combining CpG methylation from both strands ...\r\n", "@ Mon Dec 2 13:26:05 2013: writing /Volumes/web/cnidarian/BiGo_mito_methratio_A.txt ...\r\n" ] }, { "output_type": "stream", "stream": "stdout", "text": [ "@ Mon Dec 2 13:26:05 2013: done.\r\n", "total 22760 valid mappings, 6030 covered cytosines, average coverage: 39.76 fold.\r\n" ] } ], "prompt_number": 2 }, { "cell_type": "code", "collapsed": false, "input": [ "!head /Volumes/web/cnidarian/BiGo_mito_methratio_A.txt" ], "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": [ "chr\tpos\tstrand\tcontext\tratio\teff_CT_count\tC_count\tCT_count\trev_G_count\trev_GA_count\tCI_lower\tCI_upper\r\n", "gi|7212445|ref|NC_001276.1|\t9\t-\tATGGG\t0.000\t5.00\t0\t5\t9\t9\t-0.000\t0.434\r\n", "gi|7212445|ref|NC_001276.1|\t10\t-\tTGGGT\t0.000\t5.00\t0\t5\t10\t10\t-0.000\t0.434\r\n", "gi|7212445|ref|NC_001276.1|\t11\t-\tGGGTG\t0.000\t5.00\t0\t5\t10\t10\t-0.000\t0.434\r\n", "gi|7212445|ref|NC_001276.1|\t13\t-\tGTGCA\t0.000\t7.00\t0\t7\t11\t11\t0.000\t0.354\r\n", "gi|7212445|ref|NC_001276.1|\t14\t+\tTGCAA\t0.000\t11.00\t0\t11\t7\t7\t0.000\t0.259\r\n", "gi|7212445|ref|NC_001276.1|\t18\t+\tAACTT\t0.000\t14.00\t0\t14\t9\t9\t0.000\t0.215\r\n", "gi|7212445|ref|NC_001276.1|\t23\t-\tATGGG\t0.000\t11.00\t0\t11\t16\t16\t0.000\t0.259\r\n", "gi|7212445|ref|NC_001276.1|\t24\t-\tTGGGG\t0.000\t12.00\t0\t12\t16\t16\t0.000\t0.243\r\n", "gi|7212445|ref|NC_001276.1|\t25\t-\tGGGGA\t0.000\t12.00\t0\t12\t16\t16\t0.000\t0.243\r\n" ] } ], "prompt_number": 3 }, { "cell_type": "code", "collapsed": false, "input": [ "!fgrep -c \"C\" /Volumes/web/cnidarian/Cgigas_mito_genome.fasta" ], "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": [ "260\r\n" ] } ], "prompt_number": 2 }, { "cell_type": "code", "collapsed": false, "input": [ "!head /Volumes/web/cnidarian/Cgigas_mito_genome.fasta" ], "language": "python", "metadata": {}, "outputs": [ { "output_type": "stream", "stream": "stdout", "text": [ "\r\n", "ATAATTATGGGTGCAAACTTATGGGGAGTTGCAGCGATGTTCATTTGCTGAGTTAATGAGATTAGGCTTG\r\n", "ATAGCTTATATTGAGGTATTCCTCTTTTTGTACTAACTTTTTATAGTTGAGTACGTGACATTATTAATGA\r\n", "AGCAACATTTCAGGGGTTTCATACTGAAAAAGTTCAGTCAGGGCTTACTTTGGGTTTTATTCTGTTCCTA\r\n", "ATTTCTGAGTTAATATTATTTTTTTCATTTTTTTGAGCATTTTTCCATAGGGCTTTGTCATCTTCTGTTG\r\n", "AGATTGGGTGCTGCTGACCACCAGTCGGGCTAGAGTGTTTAGACTGAAGAAAAGTGCCATTACATAATAC\r\n", "AGCATTATTAGTAGCATCTTCTGCAAGTATTACTTTAAGACATAACTACCTGCAATGGGGGGACATTTGG\r\n", "GTAGCAACAGCAACGTATATTGCTACTTTAGGCTTGTCGGTAATATTTATTAAGAATCAATACGAAGAGT\r\n", "ATGCCTGATCTAGGTTTTCTATTTCTGATGGTGTATATGGCAGATGTTTTTTTATGTTAACAGGCCTTCA\r\n", "TGGTTTACATGTAATTGGAGGAACTTGTGGTCTTCTGTTTTGTTTTGTTCGTATAATGCTTTTGCAATTT\r\n" ] } ], "prompt_number": 3 }, { "cell_type": "heading", "level": 2, "metadata": {}, "source": [ "Upload and analysis in SQLShare" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "```\n", "SELECT [chr]\n", " , CAST([pos] as INTEGER) as [pos]\n", " , [strand]\n", " , [context]\n", " , CAST([ratio] as FLOAT) as [ratio]\n", " , CAST([eff_CT_count] as INTEGER) as [eff_CT_count]\n", " , CAST([C_count] as INTEGER) as [C_count]\n", " , CAST([CT_count] as INTEGER) as [CT_count]\n", " , CAST([rev_G_count] as INTEGER) as [rev_G_count]\n", " , CAST([CI_lower] as FLOAT) as [CI_lower]\n", " , CAST([CI_upper] as FLOAT) as [CI_upper]\n", " FROM [sr320@washington.edu].[BiGo_mito_methratio_A.txt]\n", " WHERE [ratio] <> 'NA'\n", "```" ] }, { "cell_type": "markdown", "metadata": {}, "source": [ "new file\n", "\n", "" ] }, { "cell_type": "code", "collapsed": false, "input": [], "language": "python", "metadata": {}, "outputs": [] } ], "metadata": {} } ] }