Model depicting mesodermal specification based on the literature and highly enriched gene expression profiles via comparison across dozens of independent induced and embryonic pluripotent stem cell lines, following differentiation to multiple lineages (ectoderm, mesoderm, endoderm, embryoid body). The underlying genomic data can be obtained from:
https://www.synapse.org/#!Synapse:syn1773109.
Proteins on this pathway have targeted assays available via the [https://assays.cancer.gov/available_assays?wp_id=WP2857 CPTAC Assay Portal]Developed using Gene Ontology mesoderm process associated genes and interactions from the software NetPerspective (AltAnalyze). A preliminary layout was obtained using the exported SIF file in Cytoscape's Cerebral plugin.d3bbbdbbda6be26c87f37c8eregulatory pathwayPW:0000004Pathway Ontology15986483PubMedDll1 is a downstream target of Tbx6 in the paraxial mesoderm.Genesis2005White PHChapman DL10617567PubMedT (Brachyury) is a direct target of Wnt3a during paraxial mesoderm specification.Genes Dev1999Yamaguchi TPTakada SYoshikawa YWu NMcMahon AP21331042PubMedTbx6-dependent Sox2 regulation determines neural or mesodermal fate in axial stem cells.Nature2011Takemoto TUchikawa MYoshida MBell DMLovell-Badge RPapaioannou VEKondoh H11260262PubMedHes7: a bHLH-type repressor gene regulated by Notch and expressed in the presomitic mesoderm.Genes Cells2001Bessho YMiyoshi GSakata RKageyama R19561075PubMedSmad6 inhibits the transcriptional activity of Tbx6 by mediating its degradation.J Biol Chem2009Chen YLLiu BZhou ZNHu RYFei CXie ZHDing X22545159PubMedmiR-125b promotes early germ layer specification through Lin28/let-7d and preferential differentiation of mesoderm in embryonic stem cells.PLoS One2012Wong SSRitner CRamachandran SAurigui JPitt CChandra PLing VBYabut OBernstein HS21266536PubMedThe Nodal inhibitor Lefty is negatively modulated by the microRNA miR-302 in embryonic stem cells.FASEB J2011Barroso-delJesus ALucena-Aguilar GSanchez LLigero GGutierrez-Aranda IMenendez P